##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931016_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1352640 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 23.147847172935887 31.0 10.0 31.0 2.0 33.0 2 21.802745002365743 30.0 2.0 31.0 2.0 34.0 3 22.573944286728175 30.0 10.0 31.0 2.0 34.0 4 24.212226460846935 33.0 2.0 37.0 2.0 37.0 5 23.600359297374023 33.0 2.0 37.0 2.0 37.0 6 24.634500680151408 35.0 8.0 37.0 2.0 37.0 7 24.93344274899456 33.0 10.0 37.0 2.0 37.0 8 25.4331425952212 35.0 11.0 37.0 2.0 37.0 9 27.00137952448545 35.0 17.0 39.0 2.0 39.0 10-11 27.46546605157322 35.0 17.0 39.0 2.0 39.0 12-13 27.420468860894253 35.0 16.0 39.0 2.0 39.0 14-15 27.532493494203926 36.0 10.0 39.0 2.0 41.0 16-17 26.377047847172935 35.5 2.0 39.0 2.0 41.0 18-19 25.2506967855453 34.0 2.0 39.0 2.0 40.5 20-21 24.912653773361722 34.0 2.0 39.0 2.0 40.0 22-23 24.58188764194464 33.0 2.0 39.0 2.0 40.0 24-25 24.45832963685829 33.0 2.0 39.0 2.0 40.0 26-27 24.1905089306837 33.0 2.0 38.5 2.0 40.0 28-29 24.0378212236811 32.0 2.0 38.0 2.0 40.0 30-31 24.124161269813108 32.0 2.0 38.0 2.0 40.0 32-33 24.04626175479063 32.5 2.0 38.0 2.0 40.0 34-35 23.753305757629526 31.5 2.0 38.0 2.0 40.0 36-37 23.263734992311335 31.0 2.0 38.0 2.0 40.0 38-39 23.21324742725337 31.0 2.0 38.0 2.0 40.0 40-41 23.148864073219777 30.5 2.0 38.0 2.0 40.0 42-43 23.053687603501302 30.5 2.0 38.0 2.0 40.0 44-45 22.695363141708068 30.0 2.0 38.0 2.0 40.0 46-47 22.367079562928794 30.0 2.0 38.0 2.0 40.0 48-49 22.198847069434585 30.0 2.0 38.0 2.0 40.0 50-51 19.385876877809324 20.0 2.0 36.0 2.0 39.0 52-53 21.64870438549799 28.5 2.0 37.0 2.0 39.0 54-55 22.570552031582682 31.0 2.0 38.0 2.0 40.0 56-57 22.598122560326473 31.0 2.0 38.0 2.0 40.0 58-59 22.34818835758221 30.5 2.0 38.0 2.0 40.0 60-61 22.078410367873197 30.0 2.0 38.0 2.0 40.0 62-63 21.65492703158268 29.0 2.0 37.0 2.0 40.0 64-65 21.34546516441921 28.0 2.0 37.0 2.0 39.5 66-67 21.028560444759876 28.0 2.0 36.0 2.0 39.0 68-69 20.59600632836527 27.5 2.0 36.0 2.0 39.0 70-71 20.271458037615332 26.5 2.0 35.0 2.0 39.0 72-73 19.8888214898273 26.0 2.0 35.0 2.0 38.0 74-75 19.456527235628105 26.0 2.0 34.5 2.0 37.5 76-77 19.076470088123965 25.0 2.0 34.0 2.0 37.0 78-79 18.732026259758698 24.5 2.0 34.0 2.0 36.0 80-81 18.332918219186183 23.5 2.0 34.0 2.0 36.0 82-83 17.902761266855926 21.5 2.0 34.0 2.0 35.5 84-85 17.554278669860423 20.0 2.0 33.0 2.0 35.0 86-87 17.091779039507927 18.0 2.0 33.0 2.0 35.0 88-89 16.717786328956706 14.5 2.0 32.5 2.0 35.0 90-91 16.275600677194227 7.0 2.0 32.0 2.0 35.0 92-93 15.75307694582446 2.0 2.0 32.0 2.0 34.5 94-95 15.222959545777147 2.0 2.0 32.0 2.0 34.0 96-97 14.590761769576531 2.0 2.0 31.0 2.0 34.0 98-99 13.893469067896854 2.0 2.0 31.0 2.0 34.0 100-101 12.704294934350603 2.0 2.0 30.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 8.522984367347703 1101 2 10.324983169969656 1101 3 9.608714650317275 1101 4 11.872290525239901 1101 5 12.572273708395041 1101 6 11.609575623656138 1101 7 11.190649640900588 1101 8 10.53242767699049 1101 9 10.517015508016414 1101 10-11 9.785218790945478 1101 12-13 9.69979596669916 1101 14-15 10.781179987309773 1101 16-17 12.058367575628619 1101 18-19 13.110355692246461 1101 20-21 13.246261671415681 1101 22-23 13.531722928725486 1101 24-25 13.448582129042894 1101 26-27 13.565979574429957 1101 28-29 13.393708574764133 1101 30-31 12.927411229741235 1101 32-33 13.051412789422962 1101 34-35 12.905695972822922 1101 36-37 13.57748322216348 1101 38-39 13.252707171982038 1101 40-41 12.801363004663486 1101 42-43 12.74439078779778 1101 44-45 12.697742326949157 1101 46-47 12.62050231924102 1101 48-49 12.594615072132846 1101 50-51 14.561041471494129 1101 52-53 13.182043870400197 1101 54-55 12.632550677897331 1101 56-57 12.751874137193763 1101 58-59 12.49160195931955 1101 60-61 12.501161493647945 1101 62-63 12.746007767440592 1101 64-65 12.512793710311946 1101 66-67 12.13141688294208 1101 68-69 11.99533349862153 1101 70-71 11.95264131014195 1101 72-73 11.904350984898308 1101 74-75 11.701257063776133 1101 76-77 11.655630782106368 1101 78-79 11.660735563150805 1101 80-81 11.669320953791882 1101 82-83 11.216229689586608 1101 84-85 10.947326670217354 1101 86-87 11.12869002766585 1101 88-89 11.036264612267551 1101 90-91 10.764467016432748 1101 92-93 10.664346567706021 1101 94-95 10.323184724021294 1101 96-97 10.52909282693737 1101 98-99 10.40774873024647 1101 100-101 9.943299988152887 1102 1 8.46749194310528 1102 2 9.942094386299622 1102 3 9.339226803095052 1102 4 11.270463662866167 1102 5 12.09117496049942 1102 6 11.098331673740315 1102 7 10.852639329452778 1102 8 10.251134274212713 1102 9 10.096830585878372 1102 10-11 9.28497547234278 1102 12-13 9.30828844355101 1102 14-15 10.028072085373747 1102 16-17 11.381284899913137 1102 18-19 12.321877814510774 1102 20-21 12.601744231390427 1102 22-23 12.791942967656468 1102 24-25 12.661055824329093 1102 26-27 12.738489175650496 1102 28-29 12.58205821702002 1102 30-31 12.422139107476934 1102 32-33 12.57973573239434 1102 34-35 12.46803617168656 1102 36-37 12.868126635463142 1102 38-39 12.579167741216047 1102 40-41 12.16595554464665 1102 42-43 11.849252556526732 1102 44-45 11.974909835366663 1102 46-47 12.022390997692206 1102 48-49 11.831417339599177 1102 50-51 13.739165813876284 1102 52-53 12.480449804743632 1102 54-55 12.009748436735723 1102 56-57 12.086427351629784 1102 58-59 12.099806399555238 1102 60-61 11.913897578454343 1102 62-63 11.978012712726791 1102 64-65 11.68861375976481 1102 66-67 11.690664305080123 1102 68-69 11.668865695591226 1102 70-71 11.066195471547676 1102 72-73 10.952443893147464 1102 74-75 10.755058094920916 1102 76-77 10.800870286525555 1102 78-79 10.52588313259525 1102 80-81 10.24701718695686 1102 82-83 10.12915385309671 1102 84-85 10.17253908078133 1102 86-87 9.992660461167535 1102 88-89 9.770685645516707 1102 90-91 9.812518494757663 1102 92-93 9.535458073387838 1102 94-95 9.64361296476204 1102 96-97 9.658873708671377 1102 98-99 9.035103659749838 1102 100-101 8.441794043287569 1103 1 8.029126214776415 1103 2 9.610705450866199 1103 3 9.047663116209442 1103 4 11.096757717267419 1103 5 11.941067093542312 1103 6 10.96468660839179 1103 7 10.493187530971987 1103 8 9.811977870615753 1103 9 9.762549489475333 1103 10-11 9.153948537021066 1103 12-13 8.87955219746075 1103 14-15 9.794704192724058 1103 16-17 10.970375501175422 1103 18-19 12.00470445241292 1103 20-21 12.489916043555311 1103 22-23 12.423122627229745 1103 24-25 12.328609164364831 1103 26-27 12.353024509933338 1103 28-29 12.00665258140787 1103 30-31 11.825242987894715 1103 32-33 12.029985152914897 1103 34-35 11.865629761107915 1103 36-37 12.180371804140535 1103 38-39 11.800236531709785 1103 40-41 11.735582094579627 1103 42-43 11.558707322221554 1103 44-45 11.472312650380065 1103 46-47 11.411040182231972 1103 48-49 11.428245387800708 1103 50-51 13.371464287681018 1103 52-53 11.948536545136836 1103 54-55 11.452671456731249 1103 56-57 11.279368698535503 1103 58-59 11.214120938205827 1103 60-61 11.201685049131289 1103 62-63 11.267344007409541 1103 64-65 11.162637546348812 1103 66-67 11.133671105236516 1103 68-69 11.096682105332068 1103 70-71 10.821095738247852 1103 72-73 10.75089326200359 1103 74-75 10.49777271176631 1103 76-77 10.322036122372296 1103 78-79 9.87052853306442 1103 80-81 9.859746884231214 1103 82-83 9.51855793179421 1103 84-85 8.991950071342096 1103 86-87 8.826088047181834 1103 88-89 8.76656470885451 1103 90-91 8.65685582859368 1103 92-93 8.468743756754685 1103 94-95 8.436892399525219 1103 96-97 8.326488138407745 1103 98-99 8.094434849707385 1103 100-101 7.36494494532063 1104 1 8.021459286923541 1104 2 9.830806364783676 1104 3 9.103224625561197 1104 4 11.18441438507018 1104 5 12.058028151030218 1104 6 11.165917990090247 1104 7 10.585973345398607 1104 8 10.037917701746576 1104 9 9.850174533835997 1104 10-11 9.300659347670432 1104 12-13 9.086619421871244 1104 14-15 9.752234349189823 1104 16-17 10.936840597679623 1104 18-19 12.198036122587443 1104 20-21 12.517917283896978 1104 22-23 12.70053048881745 1104 24-25 12.445681343114583 1104 26-27 12.413677832678669 1104 28-29 12.454465996072017 1104 30-31 12.188332809011438 1104 32-33 12.101237599339512 1104 34-35 12.237756420020983 1104 36-37 12.225339460386692 1104 38-39 11.95402396595938 1104 40-41 11.533580769908774 1104 42-43 11.212175008334295 1104 44-45 11.390170320294576 1104 46-47 11.470556817919373 1104 48-49 11.30546825225209 1104 50-51 13.418435640356186 1104 52-53 12.175386867871634 1104 54-55 11.924296444872457 1104 56-57 11.480426590516853 1104 58-59 11.539526596693737 1104 60-61 11.192504588202045 1104 62-63 11.071853158882316 1104 64-65 11.054475711626978 1104 66-67 10.657432169049105 1104 68-69 10.81593797060215 1104 70-71 10.661038078171746 1104 72-73 10.597637277267172 1104 74-75 10.415210931858443 1104 76-77 10.074592350609219 1104 78-79 9.728508972208619 1104 80-81 9.513473267668964 1104 82-83 9.436134504445555 1104 84-85 9.293348266697667 1104 86-87 9.032943131001524 1104 88-89 8.914041948760481 1104 90-91 8.584136405826909 1104 92-93 8.509046516075694 1104 94-95 8.475611790668546 1104 96-97 7.982310014099509 1104 98-99 7.618614045698939 1104 100-101 6.681866297820115 1105 1 7.992836431294254 1105 2 9.417559855459725 1105 3 8.842266706310276 1105 4 11.022580829860917 1105 5 11.395047376838004 1105 6 10.805788407664199 1105 7 10.292456422628625 1105 8 9.851369059317701 1105 9 9.718565124920367 1105 10-11 9.098833945111807 1105 12-13 8.93734335660876 1105 14-15 9.674125850137525 1105 16-17 10.870414554604732 1105 18-19 11.533571445580325 1105 20-21 12.064668177420614 1105 22-23 12.312025296232576 1105 24-25 12.396558613042995 1105 26-27 12.291379903176743 1105 28-29 12.049875228174884 1105 30-31 11.68280440029189 1105 32-33 11.51613613839828 1105 34-35 11.791862758792856 1105 36-37 11.919194958953959 1105 38-39 11.839428055356464 1105 40-41 11.600794988544287 1105 42-43 11.29687958654642 1105 44-45 11.05734093772887 1105 46-47 10.917588668295878 1105 48-49 10.958111585826217 1105 50-51 12.981212340287044 1105 52-53 11.650048722066469 1105 54-55 11.554906122103173 1105 56-57 11.147904652318761 1105 58-59 11.285184106248156 1105 60-61 11.172942956571138 1105 62-63 11.268787401046996 1105 64-65 11.19062583735667 1105 66-67 11.006573302783536 1105 68-69 10.629639153596475 1105 70-71 10.331402677092292 1105 72-73 10.177560773068727 1105 74-75 9.823740427598082 1105 76-77 9.714151023894324 1105 78-79 9.516794180823599 1105 80-81 9.29966441464715 1105 82-83 9.230231916580966 1105 84-85 9.017634737769516 1105 86-87 8.64375339095756 1105 88-89 8.520957340070513 1105 90-91 8.483375921758979 1105 92-93 8.345848595861595 1105 94-95 8.053820477885397 1105 96-97 7.733005311361664 1105 98-99 7.429017189113354 1105 100-101 7.040101334954237 1106 1 7.556352344360604 1106 2 9.251546267884468 1106 3 8.556723318030045 1106 4 10.421220307256437 1106 5 11.226177009507492 1106 6 10.401741170630029 1106 7 9.475861771169882 1106 8 9.049113024072614 1106 9 8.6523477590178 1106 10-11 8.21468267434345 1106 12-13 8.136427016040368 1106 14-15 8.746532684874982 1106 16-17 10.11115186324815 1106 18-19 11.305930358433098 1106 20-21 11.481497010685992 1106 22-23 11.653390399216093 1106 24-25 11.26623998987493 1106 26-27 11.53913185491243 1106 28-29 11.48363315117156 1106 30-31 11.316414012557072 1106 32-33 11.362975853373385 1106 34-35 11.175168777534463 1106 36-37 11.27510254473237 1106 38-39 10.97197200140154 1106 40-41 10.807099363997693 1106 42-43 10.581543303766704 1106 44-45 10.188742215998808 1106 46-47 10.081109964852033 1106 48-49 10.092794807165312 1106 50-51 12.087297757736962 1106 52-53 10.918494455606666 1106 54-55 10.251941628424802 1106 56-57 10.063387613132253 1106 58-59 9.786971027721574 1106 60-61 9.762220412810429 1106 62-63 10.178101853334276 1106 64-65 10.102296448974066 1106 66-67 9.705365062337467 1106 68-69 9.403914941836465 1106 70-71 9.041263465341107 1106 72-73 8.882550826850672 1106 74-75 8.672897249538714 1106 76-77 8.441542352416054 1106 78-79 8.341830982712938 1106 80-81 7.7708898822606045 1106 82-83 7.661243377236477 1106 84-85 7.499363967648062 1106 86-87 7.173366335691185 1106 88-89 6.7627720085799226 1106 90-91 6.841601055772573 1106 92-93 6.629984113303218 1106 94-95 6.5125692666001935 1106 96-97 6.099938019504149 1106 98-99 5.789300404804196 1106 100-101 5.2385433883055015 1107 1 7.588417065490681 1107 2 8.886195201275143 1107 3 8.596016237618862 1107 4 10.45685715279718 1107 5 11.452982891907961 1107 6 10.27558102704472 1107 7 10.15708344939047 1107 8 8.844191510249654 1107 9 6.0637804426318205 1107 10-11 7.895020636728624 1107 12-13 8.212818268039218 1107 14-15 8.795276448135201 1107 16-17 9.84721038736863 1107 18-19 11.254098640195021 1107 20-21 11.600011452268262 1107 22-23 11.380005262521841 1107 24-25 11.263717011467495 1107 26-27 11.453498511014313 1107 28-29 11.569053029547838 1107 30-31 11.063530320065933 1107 32-33 11.119779140966884 1107 34-35 11.23836579521237 1107 36-37 11.613591074159189 1107 38-39 11.403277404795261 1107 40-41 11.084370201689367 1107 42-43 10.691318453417036 1107 44-45 10.366306697849975 1107 46-47 10.601979754924066 1107 48-49 10.520954343799225 1107 50-51 12.643004178223187 1107 52-53 11.039462516136599 1107 54-55 10.672085545936877 1107 56-57 10.408538691054577 1107 58-59 10.567129149332725 1107 60-61 10.276371762783448 1107 62-63 10.212232992544322 1107 64-65 10.208280172620839 1107 66-67 10.408617235376074 1107 68-69 10.057011695813745 1107 70-71 9.534468707389909 1107 72-73 9.285052301580933 1107 74-75 9.408458809762926 1107 76-77 9.156116952218706 1107 78-79 8.847236082361608 1107 80-81 8.40878322222611 1107 82-83 8.653999915207304 1107 84-85 8.534587350001399 1107 86-87 8.207007020468822 1107 88-89 8.213205359301753 1107 90-91 7.697737415123706 1107 92-93 7.295434482906087 1107 94-95 7.139270369078906 1107 96-97 6.822319935585037 1107 98-99 6.673862021593406 1107 100-101 6.473123141807818 1108 1 6.7734522362204785 1108 2 8.498675099489805 1108 3 8.191400403555175 1108 4 9.842074252078842 1108 5 11.410591446099286 1108 6 10.093862252728048 1108 7 9.198152068034915 1108 8 6.341016634045708 1108 9 7.19175885147142 1108 10-11 6.12501759700195 1108 12-13 5.30856670062327 1108 14-15 7.492621601818872 1108 16-17 9.114664698183415 1108 18-19 8.733139770245263 1108 20-21 8.993259254609583 1108 22-23 9.89349814510193 1108 24-25 9.75279811301348 1108 26-27 8.715129561003256 1108 28-29 10.198779736277011 1108 30-31 10.327531037223007 1108 32-33 10.800118662055219 1108 34-35 10.408180945246546 1108 36-37 10.331239599846242 1108 38-39 9.992365514094367 1108 40-41 10.442624288256056 1108 42-43 9.43313508145005 1108 44-45 8.97650748362842 1108 46-47 9.807746953724923 1108 48-49 10.382522779058963 1108 50-51 12.152460777023862 1108 52-53 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2313 44-45 -19.493840164633337 2313 46-47 -19.167713168974466 2313 48-49 -19.00875193123415 2313 50-51 -16.417409706957052 2313 52-53 -18.516946028589704 2313 54-55 -19.350277604930948 2313 56-57 -19.38785650253688 2313 58-59 -19.15149568377809 2313 60-61 -18.901532109045657 2313 62-63 -18.492039370606545 2313 64-65 -18.19192009440186 2313 66-67 -17.88811173658654 2313 68-69 -17.47436347107544 2313 70-71 -17.15678629928566 2313 72-73 -16.8040665235192 2313 74-75 -16.384264821745752 2313 76-77 -16.01911669264111 2313 78-79 -15.69481999240475 2313 80-81 -15.314180729709804 2313 82-83 -14.916093542736622 2313 84-85 -14.612942690052005 2313 86-87 -14.154474955499133 2313 88-89 -13.805486229483293 2313 90-91 -13.386374734409001 2313 92-93 -12.884138280778826 2313 94-95 -12.369407868571296 2313 96-97 -11.797506499661958 2313 98-99 -11.158581236083498 2313 100-101 -10.043231998379099 2314 1 -20.167924723561388 2314 2 -18.775353530367045 2314 3 -19.54684090523828 2314 4 -20.99841654546717 2314 5 -20.25062191450058 2314 6 -21.415340201259685 2314 7 -21.754131503880558 2314 8 -22.31201676745395 2314 9 -23.863455872454963 2314 10-11 -24.40838390265722 2314 12-13 -24.415018848115654 2314 14-15 -24.47908937295959 2314 16-17 -23.196514579253535 2314 18-19 -21.979879997989226 2314 20-21 -21.606263581109573 2314 22-23 -21.258512761510204 2314 24-25 -21.12279834233758 2314 26-27 -20.895299886849504 2314 28-29 -20.74736886631331 2314 30-31 -20.849019746689738 2314 32-33 -20.80144916343899 2314 34-35 -20.491273724146776 2314 36-37 -19.992550447870187 2314 38-39 -19.97389215461729 2314 40-41 -19.93365383035335 2314 42-43 -19.832713589306596 2314 44-45 -19.493840164633337 2314 46-47 -19.167713168974466 2314 48-49 -19.00875193123415 2314 50-51 -16.417409706957052 2314 52-53 -18.516946028589704 2314 54-55 -19.350277604930948 2314 56-57 -19.38785650253688 2314 58-59 -19.15149568377809 2314 60-61 -18.901532109045657 2314 62-63 -18.492039370606545 2314 64-65 -18.19192009440186 2314 66-67 -17.88811173658654 2314 68-69 -17.47436347107544 2314 70-71 -17.15678629928566 2314 72-73 -16.8040665235192 2314 74-75 -16.384264821745752 2314 76-77 -16.01911669264111 2314 78-79 -15.69481999240475 2314 80-81 -15.314180729709804 2314 82-83 -14.916093542736622 2314 84-85 -14.612942690052005 2314 86-87 -14.154474955499133 2314 88-89 -13.805486229483293 2314 90-91 -13.386374734409001 2314 92-93 -12.884138280778826 2314 94-95 -12.369407868571296 2314 96-97 -11.797506499661958 2314 98-99 -11.158581236083498 2314 100-101 -10.043231998379099 2315 1 -20.167924723561388 2315 2 -18.775353530367045 2315 3 -19.54684090523828 2315 4 -20.99841654546717 2315 5 -20.25062191450058 2315 6 -21.415340201259685 2315 7 -21.754131503880558 2315 8 -22.31201676745395 2315 9 -23.863455872454963 2315 10-11 -24.40838390265722 2315 12-13 -24.415018848115654 2315 14-15 -24.47908937295959 2315 16-17 -23.196514579253535 2315 18-19 -21.979879997989226 2315 20-21 -21.606263581109573 2315 22-23 -21.258512761510204 2315 24-25 -21.12279834233758 2315 26-27 -20.895299886849504 2315 28-29 -20.74736886631331 2315 30-31 -20.849019746689738 2315 32-33 -20.80144916343899 2315 34-35 -20.491273724146776 2315 36-37 -19.992550447870187 2315 38-39 -19.97389215461729 2315 40-41 -19.93365383035335 2315 42-43 -19.832713589306596 2315 44-45 -19.493840164633337 2315 46-47 -19.167713168974466 2315 48-49 -19.00875193123415 2315 50-51 -16.417409706957052 2315 52-53 -18.516946028589704 2315 54-55 -19.350277604930948 2315 56-57 -19.38785650253688 2315 58-59 -19.15149568377809 2315 60-61 -18.901532109045657 2315 62-63 -18.492039370606545 2315 64-65 -18.19192009440186 2315 66-67 -17.88811173658654 2315 68-69 -17.47436347107544 2315 70-71 -17.15678629928566 2315 72-73 -16.8040665235192 2315 74-75 -16.384264821745752 2315 76-77 -16.01911669264111 2315 78-79 -15.69481999240475 2315 80-81 -15.314180729709804 2315 82-83 -14.916093542736622 2315 84-85 -14.612942690052005 2315 86-87 -14.154474955499133 2315 88-89 -13.805486229483293 2315 90-91 -13.386374734409001 2315 92-93 -12.884138280778826 2315 94-95 -12.369407868571296 2315 96-97 -11.797506499661958 2315 98-99 -11.158581236083498 2315 100-101 -10.043231998379099 2316 1 -20.167924723561388 2316 2 -18.775353530367045 2316 3 -19.54684090523828 2316 4 -20.99841654546717 2316 5 -20.25062191450058 2316 6 -21.415340201259685 2316 7 -21.754131503880558 2316 8 -22.31201676745395 2316 9 -23.863455872454963 2316 10-11 -24.40838390265722 2316 12-13 -24.415018848115654 2316 14-15 -24.47908937295959 2316 16-17 -23.196514579253535 2316 18-19 -21.979879997989226 2316 20-21 -21.606263581109573 2316 22-23 -21.258512761510204 2316 24-25 -21.12279834233758 2316 26-27 -20.895299886849504 2316 28-29 -20.74736886631331 2316 30-31 -20.849019746689738 2316 32-33 -20.80144916343899 2316 34-35 -20.491273724146776 2316 36-37 -19.992550447870187 2316 38-39 -19.97389215461729 2316 40-41 -19.93365383035335 2316 42-43 -19.832713589306596 2316 44-45 -19.493840164633337 2316 46-47 -19.167713168974466 2316 48-49 -19.00875193123415 2316 50-51 -16.417409706957052 2316 52-53 -18.516946028589704 2316 54-55 -19.350277604930948 2316 56-57 -19.38785650253688 2316 58-59 -19.15149568377809 2316 60-61 -18.901532109045657 2316 62-63 -18.492039370606545 2316 64-65 -18.19192009440186 2316 66-67 -17.88811173658654 2316 68-69 -17.47436347107544 2316 70-71 -17.15678629928566 2316 72-73 -16.8040665235192 2316 74-75 -16.384264821745752 2316 76-77 -16.01911669264111 2316 78-79 -15.69481999240475 2316 80-81 -15.314180729709804 2316 82-83 -14.916093542736622 2316 84-85 -14.612942690052005 2316 86-87 -14.154474955499133 2316 88-89 -13.805486229483293 2316 90-91 -13.386374734409001 2316 92-93 -12.884138280778826 2316 94-95 -12.369407868571296 2316 96-97 -11.797506499661958 2316 98-99 -11.158581236083498 2316 100-101 -10.043231998379099 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 289306.0 3 11460.0 4 48750.0 5 23863.0 6 10679.0 7 10923.0 8 10808.0 9 9870.0 10 9987.0 11 11134.0 12 12049.0 13 12700.0 14 13273.0 15 14055.0 16 15232.0 17 15815.0 18 14879.0 19 14449.0 20 15618.0 21 16617.0 22 18139.0 23 19805.0 24 21822.0 25 23875.0 26 26414.0 27 29265.0 28 32484.0 29 36484.0 30 41858.0 31 47769.0 32 55843.0 33 65418.0 34 76748.0 35 88137.0 36 93158.0 37 70655.0 38 22485.0 39 814.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.54020715744467 20.140647341498017 13.77625771180378 23.542887789253534 2 15.00068786365733 20.726040090312452 41.56830890742974 22.70496313860048 3 18.33866328091206 26.238009953595352 30.578203550744743 24.84512321474784 4 12.132190724466376 17.306921635220636 39.62695871468647 30.933928925626525 5 12.372545138289315 37.96196464746438 36.06672506812333 13.598765146122972 6 29.38791537536915 38.77512029708484 18.275364155501023 13.561600172044994 7 25.46135406175974 33.19328406549502 23.695466129543206 17.64989574320203 8 24.08164886582247 36.95964409746515 21.160872026208814 17.797835010503565 9 25.192454779335076 16.843973392402464 21.52993685827713 36.433634969985334 10-11 23.583937984656085 27.133690492465178 29.704952509184768 19.577419013693966 12-13 24.204968312486965 24.84772413507826 29.850852382897536 21.096455169537244 14-15 21.49275755614988 26.46780330823836 27.332227667268867 24.707211468342898 16-17 21.034299053924038 29.479899101445326 28.032417379135254 21.45338446549538 18-19 20.924505202519192 28.357348420352757 30.10189135677677 20.616255020351286 20-21 21.461439621072785 27.631452974225894 30.302738656118517 20.6043687485828 22-23 21.10780877064053 27.479902887510697 30.10722295400664 21.305065387842127 24-25 21.04268012398695 27.66511723944673 30.20193354751645 21.09026908904987 26-27 20.98888706388536 27.87012406908128 29.938526314102692 21.20246255293067 28-29 20.99171257928608 28.08656264950538 29.986149050110345 20.9355757210982 30-31 21.181271957929432 27.775804892176193 30.031576567635472 21.011346582258906 32-33 20.93284768721507 27.95450549760951 29.980271235207688 21.13237557996773 34-35 21.14663497704216 28.023464414875075 29.917033744498596 20.912866863584163 36-37 21.20739657119392 27.89483191585132 29.778720791578817 21.119050721375945 38-39 21.075733299616136 28.0957917383651 29.811861999205803 21.01661296281296 40-41 21.26961956223796 28.006616723434785 29.803524310976464 20.920239403350788 42-43 21.20568089425513 28.03679112176144 29.585255053435894 21.17227293054754 44-45 21.166623460852207 28.28062698758616 29.493048611763946 21.059700939797683 46-47 21.33511107428037 28.281076431022274 29.197767267862744 21.186045226834615 48-49 21.234168800817272 28.446385005434504 29.047585727547105 21.27186046620112 50-51 21.530303837444105 28.66298055999204 28.728112275820756 21.078603326743103 52-53 21.199607222233514 28.69596303528903 28.846395441856515 21.258034300620942 54-55 21.084236122325276 28.690084182294335 28.96076875729896 21.26491093808143 56-57 21.159587371353773 28.79095963780908 28.90557336887992 21.143879621957222 58-59 21.074691149106688 28.689324062451355 28.960081798539676 21.275902989902278 60-61 21.05186924293058 28.821321392394278 29.03532107051907 21.091488294156072 62-63 21.081457109816316 28.642904503172733 28.927167666116727 21.348470720894216 64-65 20.943695847998292 28.648858222746394 28.96975381443682 21.437692114818496 66-67 20.86823298612733 28.773272922602644 28.80391424167406 21.554579849595964 68-69 21.051201782476287 28.6391987927843 28.898576305651595 21.411023119087822 70-71 20.981419838675166 28.527211091187016 28.923351438318107 21.568017631819718 72-73 20.940877004079045 28.73239162544687 28.75110964496921 21.575621725504877 74-75 20.932276183118383 28.941039473050722 28.456349808072435 21.670334535758464 76-77 20.961409265225363 28.824571766095687 28.600800559589402 21.613218409089548 78-79 20.882247596016747 28.884300820148972 28.58676582978772 21.646685754046562 80-81 20.96786558782236 28.879221163082835 28.423344786817413 21.729568462277395 82-83 21.098067794698935 28.795995707556553 28.51669798709005 21.589238510654457 84-85 21.000305704243075 29.00466930589829 28.35192996626448 21.643095023594157 86-87 21.06941163802485 28.962135982386485 28.355651881058368 21.612800498530298 88-89 21.024669098154327 28.899778200955268 28.36894307127718 21.706609629613226 90-91 21.00141592255531 28.90378460386921 28.342408644136196 21.752390829439282 92-93 21.01500754614139 28.874079061576058 28.276433824118797 21.834479568163758 94-95 20.959855548718526 28.84640760418825 28.34647811578503 21.84725873130819 96-97 20.967972848549056 28.67254602119037 28.335076678868592 22.024404451391977 98-99 20.77414626020323 28.720084402243323 28.08489088788939 22.420878449664055 100-101 20.967476628060467 28.777019167460043 28.091868228073697 22.163635976405793 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 74054.0 1 66725.0 2 44144.0 3 22660.0 4 14053.0 5 10955.5 6 10264.5 7 10648.5 8 11239.5 9 11280.5 10 10314.0 11 8688.0 12 7056.0 13 5545.0 14 4300.5 15 3603.0 16 3394.5 17 3599.0 18 4090.0 19 4626.5 20 5325.5 21 6197.0 22 7026.0 23 8067.5 24 9198.5 25 10409.5 26 12045.5 27 13715.0 28 15604.5 29 17791.0 30 19902.5 31 22133.0 32 24652.5 33 26646.5 34 28321.5 35 30060.0 36 32198.0 37 34643.5 38 36227.0 39 37286.0 40 38110.5 41 38774.0 42 39436.0 43 40087.5 44 40716.5 45 41630.0 46 41724.5 47 40769.5 48 39747.5 49 38687.0 50 36805.5 51 34056.5 52 31585.0 53 29173.5 54 26145.0 55 22488.5 56 18847.0 57 15539.0 58 12543.0 59 9883.5 60 7549.0 61 5589.0 62 4019.5 63 2729.0 64 1731.0 65 1039.5 66 597.5 67 352.0 68 230.0 69 145.5 70 77.5 71 44.5 72 31.0 73 21.5 74 18.5 75 13.0 76 7.5 77 6.5 78 7.0 79 7.0 80 3.0 81 1.0 82 1.5 83 1.5 84 1.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 20.323885143127512 2 26.915661225455406 3 20.86830198722498 4 26.94671161580317 5 29.785604447598768 6 22.995919091554292 7 21.820661816891413 8 18.106887272297136 9 10.687544357700498 10-11 14.015037260468418 12-13 14.212650816181688 14-15 18.135904601372133 16-17 20.969770227111425 18-19 24.667502070026025 20-21 26.641604565885974 22-23 26.110458067187132 24-25 24.810555654128226 26-27 26.183167731251476 28-29 23.35344215755855 30-31 24.95035634019399 32-33 24.098540631653655 34-35 22.36027324343506 36-37 25.104684173172465 38-39 25.158061272770286 40-41 22.45848858528507 42-43 23.2113126922167 44-45 22.144916607523065 46-47 25.06165720369056 48-49 20.758294889992904 50-51 26.44702211970665 52-53 19.967914596640643 54-55 21.02721344925479 56-57 23.047706706884316 58-59 22.345524308019872 60-61 21.907528980364326 62-63 21.782699018216228 64-65 20.39049562337355 66-67 20.862165838656257 68-69 21.7601874852141 70-71 20.36731872486397 72-73 20.217204873432696 74-75 17.847579548142892 76-77 19.83332594038325 78-79 18.985280636385145 80-81 19.96096522356281 82-83 20.670060030754673 84-85 17.897555890702627 86-87 19.327574225218832 88-89 18.220553879820205 90-91 19.304877868464633 92-93 19.297263129879347 94-95 18.48122929973977 96-97 17.377646676129643 98-99 16.787726224272532 100-101 18.425708244617933 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1352640.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.98225026115126 #Duplication Level Percentage of deduplicated Percentage of total 1 89.05917693209562 70.34094200502928 2 7.4725044013135875 11.80390425404208 3 1.744637135736326 4.133861006088738 4 0.7063924699138555 2.231698673653156 5 0.34918419252292365 1.3789676640541788 6 0.19449502303908908 0.921699275053303 7 0.11633394836885542 0.6431821916755753 8 0.08303248017701004 0.524647370331575 9 0.05975879941292328 0.4247896005483701 >10 0.20567826831510996 2.5416482067824075 >50 0.005691428453879856 0.31023586900849026 >100 0.0027373630455300485 0.33779632173151664 >500 9.438940131491063E-5 0.0556895022554092 >1k 1.8877880262982126E-4 0.2233544160069692 >5k 0.0 0.0 >10k+ 9.438940131491063E-5 4.127583643738937 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 55366 4.093180742843624 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1744 0.12893304944405015 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 7.392950082801041E-5 0.0 0.0 0.0 0.0 2 7.392950082801041E-5 0.0 0.0 0.0 0.0 3 7.392950082801041E-5 0.0 0.0 0.0 0.0 4 7.392950082801041E-5 0.0 0.0 0.0 0.0 5 7.392950082801041E-5 0.0 0.0 0.0 0.0 6 7.392950082801041E-5 0.0 0.0 0.0 0.0 7 7.392950082801041E-5 0.0 0.0 0.0 0.0 8 7.392950082801041E-5 0.0 0.0 0.0 0.0 9 7.392950082801041E-5 0.0 0.0 0.0 0.0 10-11 7.392950082801041E-5 0.0 0.0 0.0 0.0 12-13 7.392950082801041E-5 0.0 0.0 0.0 0.0 14-15 7.392950082801041E-5 0.0 0.0 0.0 0.0 16-17 1.4785900165602083E-4 0.0 0.0 0.0 0.0 18-19 1.4785900165602083E-4 0.0 0.0 0.0 0.0 20-21 1.4785900165602083E-4 0.0 0.0 0.0 0.0 22-23 1.4785900165602083E-4 0.0 0.0 0.0 0.0 24-25 1.4785900165602083E-4 0.0 0.0 0.0 0.0 26-27 1.4785900165602083E-4 0.0 0.0 3.696475041400521E-5 0.0 28-29 1.4785900165602083E-4 0.0 0.0 1.1089425124201562E-4 0.0 30-31 1.4785900165602083E-4 0.0 0.0 7.023302578660988E-4 0.0 32-33 1.4785900165602083E-4 0.0 0.0 0.0029941447835344216 0.0 34-35 1.8482375207002602E-4 0.0 0.0 0.006468831322450911 0.0 36-37 2.2178850248403122E-4 0.0 0.0 0.01264194464158978 0.0 38-39 2.2178850248403122E-4 0.0 0.0 0.02236367400047315 0.0 40-41 2.2178850248403122E-4 0.0 0.0 0.03149396735273243 0.0 42-43 2.2178850248403122E-4 0.0 0.0 0.040883013957889756 0.0 44-45 2.2178850248403122E-4 0.0 0.0 0.054818724863969714 0.0 46-47 2.2178850248403122E-4 0.0 0.0 0.06978944878164184 0.0 48-49 2.2178850248403122E-4 0.0 0.0 0.08893718949609652 0.0 50-51 2.2178850248403122E-4 0.0 0.0 0.10875029571800332 0.0 52-53 2.2178850248403122E-4 0.0 0.0 0.1304486042110244 0.0 54-55 2.2178850248403122E-4 0.0 0.0 0.15373639697184766 0.0 56-57 3.6964750414005204E-4 0.0 0.0 0.1839735628105039 0.0 58-59 3.6964750414005204E-4 0.0 0.0 0.21583717766737637 0.0 60-61 3.6964750414005204E-4 0.0 0.0 0.2499556422995032 0.0 62-63 4.0661225455405724E-4 0.0 0.0 0.28943399574166073 0.0 64-65 4.4357700496806243E-4 0.0 0.0 0.33020611544830847 0.0 66-67 4.4357700496806243E-4 0.0 0.0 0.3747856044475988 0.0 68-69 4.4357700496806243E-4 0.0 0.0 0.4303805890702626 0.0 70-71 4.4357700496806243E-4 0.0 0.0 0.490448308493021 0.0 72-73 4.4357700496806243E-4 0.0 0.0 0.5548409037142181 0.0 74-75 4.4357700496806243E-4 0.0 0.0 0.6218949609652236 0.0 76-77 4.4357700496806243E-4 0.0 0.0 0.6944937307783299 0.0 78-79 4.4357700496806243E-4 0.0 0.0 0.7805476697421339 0.0 80-81 4.4357700496806243E-4 0.0 0.0 0.8717766737638988 0.0 82-83 4.4357700496806243E-4 0.0 0.0 0.9676632363378282 0.0 84-85 4.4357700496806243E-4 0.0 0.0 1.0694271942275846 0.0 86-87 4.4357700496806243E-4 0.0 0.0 1.1826502247456825 0.0 88-89 4.4357700496806243E-4 0.0 0.0 1.3047447953631417 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1285 0.0 61.45593 1 GTATCAA 2500 0.0 45.038868 1 TCAACGC 2925 0.0 37.50811 4 ATCAACG 2960 0.0 37.247746 3 TATCAAC 3010 0.0 36.38422 2 CAACGCA 3035 0.0 36.1429 5 AACGCAG 3245 0.0 33.22863 6 ACGCAGA 3630 0.0 29.434227 7 CGCAGAG 3755 0.0 28.837528 8 GTACATG 2000 0.0 25.72922 1 TAACGCA 160 1.1696266E-6 25.048223 4 GCAGAGT 4305 0.0 24.760616 9 TACATGG 2115 0.0 23.602436 2 GTGGTAT 700 0.0 23.290949 1 ACATGGG 2050 0.0 22.979626 3 CCGTGCG 100 0.0025032724 22.115032 9 CAGAGTA 4090 0.0 22.008425 10-11 TGGTATC 635 0.0 21.658636 2 GAGTACT 3035 0.0 21.436687 12-13 TATCACG 120 0.0031775648 21.224129 2 >>END_MODULE