FastQCFastQC Report
Sat 18 Jun 2016
SRR1931016_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931016_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1352640
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42340.3130175065057961No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37340.27605275609179086No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24440.18068370002365744No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18700.054.137641
GTATCAA36000.039.7397731
TAACGCA1404.5474735E-1033.943214
ATCAACG43250.032.4128233
TCAACGC43750.031.9337754
CAACGCA43700.031.9183085
TATCAAC44700.031.791352
AACGCAG45300.030.894546
ACGCAGA52950.026.3414257
CGCAGAG53700.025.796848
ATAACGC1701.1184602E-725.1579073
GCGGTAT950.001222265325.0154171
TAGCCCG1252.2745406E-422.7727385
CCTATAC4200.022.6288073
GTACATG29000.022.4534931
GCAGAGT61000.022.243049
TATAACG1954.1990825E-721.9357852
GTATAAC5350.021.321551
TGGTATC9900.020.6432722
TACATGG29000.020.3221172
Produced by FastQC (version 0.11.5)