##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931016_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1352640 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.3012590193991 31.0 31.0 34.0 30.0 34.0 2 31.45433670451857 31.0 31.0 34.0 30.0 34.0 3 31.51594807191862 31.0 31.0 34.0 30.0 34.0 4 35.297676395788976 37.0 35.0 37.0 33.0 37.0 5 35.17835787792761 37.0 35.0 37.0 32.0 37.0 6 35.20858173645612 37.0 35.0 37.0 32.0 37.0 7 35.094904778802935 37.0 35.0 37.0 32.0 37.0 8 35.094031671398156 37.0 35.0 37.0 32.0 37.0 9 36.592906464395554 39.0 35.0 39.0 32.0 39.0 10-11 36.53826701857109 38.0 35.0 39.0 32.0 39.0 12-13 36.41890044653418 38.0 35.0 39.0 32.0 39.0 14-15 37.46415269399101 39.0 36.0 41.0 32.0 41.0 16-17 37.417571563756795 39.0 36.0 41.0 32.0 41.0 18-19 37.4349819612018 39.0 36.0 41.0 32.0 41.0 20-21 37.41806393423232 39.0 36.0 41.0 32.0 41.0 22-23 37.308201738821865 39.0 36.0 40.5 31.5 41.0 24-25 37.177601579134134 39.0 36.0 40.0 31.0 41.0 26-27 36.91118220664774 39.0 36.0 40.0 31.0 41.0 28-29 36.7167945647031 39.0 35.5 40.0 30.0 41.0 30-31 36.46707475751124 38.0 35.0 40.0 30.0 41.0 32-33 36.28277036018453 38.0 35.0 40.0 30.0 41.0 34-35 36.33721167494677 38.0 35.0 40.0 30.0 41.0 36-37 36.38481746806245 38.0 35.0 40.0 30.0 41.0 38-39 36.36157551159215 38.0 35.0 40.0 30.0 41.0 40-41 36.23186102732434 38.0 35.0 40.0 30.0 41.0 42-43 36.082684601963564 38.0 35.0 40.0 29.5 41.0 44-45 35.90203675774781 38.0 35.0 40.0 29.0 41.0 46-47 35.63140118582919 38.0 35.0 40.0 27.5 41.0 48-49 35.39700068015141 38.0 34.0 40.0 27.0 41.0 50-51 35.11678347527797 38.0 34.0 40.0 26.5 41.0 52-53 34.89510290986515 38.0 34.0 40.0 26.0 41.0 54-55 34.7295182014431 38.0 33.0 40.0 26.0 41.0 56-57 34.55253689082092 38.0 33.0 40.0 25.0 41.0 58-59 34.330942083629054 38.0 33.0 40.0 25.0 41.0 60-61 34.00850558907027 37.0 33.0 40.0 23.5 41.0 62-63 33.74167332032174 37.0 32.5 40.0 23.0 41.0 64-65 33.414680550626926 36.5 32.0 39.0 23.0 41.0 66-67 33.07761932221434 36.0 31.5 39.0 22.0 40.5 68-69 32.71153928613674 36.0 31.0 39.0 22.0 40.0 70-71 32.32266160988881 35.0 31.0 38.5 21.0 40.0 72-73 31.888622619470077 35.0 31.0 38.0 20.0 40.0 74-75 31.363416725810268 35.0 30.0 37.0 18.0 39.0 76-77 30.018852392358646 33.5 28.5 35.5 17.5 38.5 78-79 30.72906981902058 34.0 30.0 36.0 18.0 39.0 80-81 30.677479595457772 34.0 30.0 36.0 18.0 38.0 82-83 30.414233277146913 34.0 30.0 35.5 18.0 37.0 84-85 30.065330021291693 34.0 30.0 35.0 14.5 37.0 86-87 29.707009255973503 34.0 29.0 35.0 9.5 36.0 88-89 29.34383649751597 34.0 29.0 35.0 7.0 36.0 90-91 28.989960373787554 34.0 29.0 35.0 2.0 36.0 92-93 28.65139541932813 34.0 29.0 35.0 2.0 35.0 94-95 28.246069168440975 33.5 29.0 35.0 2.0 35.0 96-97 27.79533985391531 33.0 27.5 35.0 2.0 35.0 98-99 27.26895256683227 33.0 27.0 35.0 2.0 35.0 100-101 26.110209294416844 32.0 24.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3350356573438624 1101 2 0.4844223215785881 1101 3 0.41113313678843255 1101 4 0.16719499838645646 1101 5 0.39273252691518223 1101 6 0.2789133974776661 1101 7 0.4019754081310225 1101 8 0.2800113620286453 1101 9 0.44615349944418625 1101 10-11 0.48365565513004327 1101 12-13 0.5321277651136356 1101 14-15 0.5127584895991433 1101 16-17 0.5797887799102952 1101 18-19 0.48673907323544796 1101 20-21 0.43118911912621627 1101 22-23 0.5186517777736341 1101 24-25 0.6240700851722636 1101 26-27 0.48859884982509527 1101 28-29 0.4680083586821837 1101 30-31 0.48987495240319134 1101 32-33 0.5857808188532729 1101 34-35 0.6065640999584119 1101 36-37 0.5294867762631412 1101 38-39 0.38035611759959664 1101 40-41 0.5229753045142758 1101 42-43 0.5082396654536652 1101 44-45 0.646642520912124 1101 46-47 0.44295705145382414 1101 48-49 0.543528204013235 1101 50-51 0.4199570769168943 1101 52-53 0.22697587503683536 1101 54-55 0.42453896422130555 1101 56-57 0.5209687919091621 1101 58-59 0.5199616037720105 1101 60-61 0.5225703766551746 1101 62-63 0.366787715782408 1101 64-65 0.5474160652335769 1101 66-67 0.6167273159074895 1101 68-69 0.2897047808891955 1101 70-71 0.28850127622815336 1101 72-73 0.23609304136770604 1101 74-75 0.020665822436026104 1101 76-77 0.08729819288515728 1101 78-79 0.1931175457533385 1101 80-81 0.31188011150410233 1101 82-83 0.47804729083136266 1101 84-85 0.343033330432295 1101 86-87 0.45706268756746127 1101 88-89 0.4158670518681973 1101 90-91 0.20146177951162514 1101 92-93 0.22807800697084346 1101 94-95 0.4525989755459001 1101 96-97 0.5412366960705484 1101 98-99 0.6328219017457855 1101 100-101 1.024797854748062 1102 1 0.35743149067719315 1102 2 0.32010072014761093 1102 3 0.357240039145335 1102 4 0.09471893567602052 1102 5 0.21464435351450817 1102 6 0.280186545625817 1102 7 0.41689807227243136 1102 8 0.3254277656481719 1102 9 0.5106263529795427 1102 10-11 0.44037979759637125 1102 12-13 0.39959409507996213 1102 14-15 0.6014586421664845 1102 16-17 0.5307515324355521 1102 18-19 0.6518853274442051 1102 20-21 0.4291077586464169 1102 22-23 0.35669233964232205 1102 24-25 0.46297015356451965 1102 26-27 0.5522805890927671 1102 28-29 0.47180873220911934 1102 30-31 0.5539321914604258 1102 32-33 0.6657307083734736 1102 34-35 0.6759625111537062 1102 36-37 0.6626609660779579 1102 38-39 0.4531215321618802 1102 40-41 0.7106956064923864 1102 42-43 0.8355301484082176 1102 44-45 0.7367572094643151 1102 46-47 0.8088700880699875 1102 48-49 0.7740771833903395 1102 50-51 0.7201694874808666 1102 52-53 0.8097285318129295 1102 54-55 0.8446022270580045 1102 56-57 0.7630523882896298 1102 58-59 0.6906265869370003 1102 60-61 0.44472880994641883 1102 62-63 0.49295630269318735 1102 64-65 0.634537277354795 1102 66-67 0.4691309091314011 1102 68-69 0.5300483204514848 1102 70-71 0.34651303443521897 1102 72-73 0.6048765798778071 1102 74-75 0.6172725143552178 1102 76-77 0.575827364812767 1102 78-79 0.6177861063593966 1102 80-81 0.5462722095680768 1102 82-83 0.4510119636007275 1102 84-85 0.583433425129261 1102 86-87 0.4702360882408634 1102 88-89 0.42500465182610725 1102 90-91 0.4028907830890667 1102 92-93 0.27653720835973417 1102 94-95 0.29917804751559274 1102 96-97 0.3775615329813249 1102 98-99 0.5569347752727225 1102 100-101 0.622296934083078 1103 1 0.34294450452884817 1103 2 0.3179555989456446 1103 3 0.40759191582093734 1103 4 0.15441753654733503 1103 5 0.1871155889926186 1103 6 0.26204814080008276 1103 7 0.4032035068122042 1103 8 0.32936368778847935 1103 9 0.5058997555711429 1103 10-11 0.3842654167317576 1103 12-13 0.4987339520951508 1103 14-15 0.5777811469140275 1103 16-17 0.6728636302907844 1103 18-19 0.6602284351296319 1103 20-21 0.5454077125673322 1103 22-23 0.5669369078952329 1103 24-25 0.6366647539331538 1103 26-27 0.6910144265034077 1103 28-29 0.5850227835384345 1103 30-31 0.5731457750171742 1103 32-33 0.7435650959404967 1103 34-35 0.7529023709615714 1103 36-37 0.754712672400565 1103 38-39 0.7557092918803789 1103 40-41 0.6385704878038325 1103 42-43 0.5042974280663941 1103 44-45 0.49779524565734334 1103 46-47 0.581516912801888 1103 48-49 0.4403092404725655 1103 50-51 0.49020603657604056 1103 52-53 0.40744499880354823 1103 54-55 0.41855305508526186 1103 56-57 0.3661875117257338 1103 58-59 0.5230104117806107 1103 60-61 0.480247234600121 1103 62-63 0.2088481774139126 1103 64-65 0.005404122752466378 1103 66-67 0.043586163543814394 1103 68-69 -0.08049611096049603 1103 70-71 0.03972666686372861 1103 72-73 0.13911386623840016 1103 74-75 0.2394170072059829 1103 76-77 0.24572044038247043 1103 78-79 0.3919497010868369 1103 80-81 0.47560538930444807 1103 82-83 0.2769002078478202 1103 84-85 0.3780341428459728 1103 86-87 0.13472447072522087 1103 88-89 0.21025079095702992 1103 90-91 0.38536763881963054 1103 92-93 0.13564428638699155 1103 94-95 0.32077063855670573 1103 96-97 0.5700263802221635 1103 98-99 0.892843475842426 1103 100-101 1.0646221477224884 1104 1 0.33922962851031 1104 2 0.4979869764276472 1104 3 0.4033982960260687 1104 4 0.21703370877563088 1104 5 0.31662324713617807 1104 6 0.3421336295052413 1104 7 0.3544304963659215 1104 8 0.3607060326315761 1104 9 0.4912572234469934 1104 10-11 0.46086969151838986 1104 12-13 0.38203730172041617 1104 14-15 0.5311132914401853 1104 16-17 0.5861148188051502 1104 18-19 0.5676407064306588 1104 20-21 0.374690317214565 1104 22-23 0.47771356206478544 1104 24-25 0.5411681077748725 1104 26-27 0.5756767057102863 1104 28-29 0.5745901221446843 1104 30-31 0.5467784125176607 1104 32-33 0.5522177967581428 1104 34-35 0.5992906225014494 1104 36-37 0.6720648064558432 1104 38-39 0.6401018934004128 1104 40-41 0.5927832573497511 1104 42-43 0.6299720614979947 1104 44-45 0.6990872389531688 1104 46-47 0.7581118762416779 1104 48-49 0.661426496682175 1104 50-51 0.6535059778969909 1104 52-53 0.5953673035200069 1104 54-55 0.5851203128760432 1104 56-57 0.6595401763521025 1104 58-59 0.7874210386626359 1104 60-61 0.7074616661336179 1104 62-63 0.677218586305024 1104 64-65 0.7344171375557522 1104 66-67 0.6153520516881912 1104 68-69 0.7706606921203303 1104 70-71 0.7505364378045982 1104 72-73 0.6987020938850144 1104 74-75 0.7553547828130611 1104 76-77 0.7663850421554912 1104 78-79 0.7641211598762183 1104 80-81 0.7683866359005407 1104 82-83 0.8523620857883607 1104 84-85 0.8231933844455561 1104 86-87 0.8614276566113297 1104 88-89 0.9945547589962729 1104 90-91 0.6654094696061073 1104 92-93 0.6575523043350486 1104 94-95 0.8389308394006996 1104 96-97 1.0301079335656418 1104 98-99 0.9647859282052238 1104 100-101 1.3964166199448087 1105 1 0.23700416128086843 1105 2 0.46114095144682565 1105 3 0.44656090528706827 1105 4 0.15336907511171916 1105 5 0.3329801474777625 1105 6 0.3592137765969454 1105 7 0.22897771466088557 1105 8 0.13028443395526068 1105 9 0.4401088004861009 1105 10-11 0.45391069900713177 1105 12-13 0.471608530775498 1105 14-15 0.4595187430523069 1105 16-17 0.4964872607820112 1105 18-19 0.5433100321686126 1105 20-21 0.5761227069731873 1105 22-23 0.5406910683168675 1105 24-25 0.43212769539523066 1105 26-27 0.33019592291822875 1105 28-29 0.43480957292434397 1105 30-31 0.4555069946100403 1105 32-33 0.4318298922645454 1105 34-35 0.5190717221117112 1105 36-37 0.5378711448837308 1105 38-39 0.5764144701540062 1105 40-41 0.5524796657115516 1105 42-43 0.5727886990971953 1105 44-45 0.4911658380732362 1105 46-47 0.5387595647760222 1105 48-49 0.5942307677079341 1105 50-51 0.5413150132354545 1105 52-53 0.5087191609139765 1105 54-55 0.6593244834556415 1105 56-57 0.6201051688014516 1105 58-59 0.7258956158083905 1105 60-61 0.47413590066163636 1105 62-63 0.44447933008819973 1105 64-65 0.620757749795736 1105 66-67 0.8206139512901913 1105 68-69 0.5104350494541023 1105 70-71 0.35035007921868555 1105 72-73 0.45632886416258245 1105 74-75 0.580629633777793 1105 76-77 0.6181168784282534 1105 78-79 0.4606133701389261 1105 80-81 0.49203069874130634 1105 82-83 0.6072799056939004 1105 84-85 0.5458011662709943 1105 86-87 0.7515681092382387 1105 88-89 0.7220257517276849 1105 90-91 0.5448358085155753 1105 92-93 0.6703789693505442 1105 94-95 0.7720752341953876 1105 96-97 0.7863823786178052 1105 98-99 0.685843588428888 1105 100-101 0.8343494070161483 1106 1 0.24680431907889755 1106 2 0.1476237145715089 1106 3 0.14294531823074053 1106 4 -0.0371596894920998 1106 5 0.10216081563062573 1106 6 0.147841639775109 1106 7 0.3549646142473293 1106 8 0.18540739815770024 1106 9 0.3372364880389469 1106 10-11 0.2775762554393424 1106 12-13 0.26830346517635206 1106 14-15 0.35616588794938053 1106 16-17 0.25201813465478295 1106 18-19 0.373586249322706 1106 20-21 0.28586567622375725 1106 22-23 0.19110665607922073 1106 24-25 0.19939744530123704 1106 26-27 0.2204662800112871 1106 28-29 0.2139658035636387 1106 30-31 0.15780464158371643 1106 32-33 0.32108952120972134 1106 34-35 0.3101545890230142 1106 36-37 0.28523411741733895 1106 38-39 0.262722711129598 1106 40-41 0.3754045510255679 1106 42-43 0.28859669913955344 1106 44-45 0.4458873792692941 1106 46-47 0.32656379814060443 1106 48-49 0.32237291654995204 1106 50-51 0.18757384083493633 1106 52-53 0.19152769224501043 1106 54-55 0.3907353372771283 1106 56-57 0.5716588351345138 1106 58-59 0.6462559301335915 1106 60-61 0.5269135166405405 1106 62-63 0.3404363292427206 1106 64-65 0.4486204425643905 1106 66-67 0.413417372718591 1106 68-69 0.34219018186148276 1106 70-71 0.506933072333112 1106 72-73 0.5086566057268307 1106 74-75 0.7037529139293106 1106 76-77 0.6991467841554027 1106 78-79 0.4356783250323417 1106 80-81 0.40103913052190165 1106 82-83 0.4798992558978554 1106 84-85 0.5835888520234569 1106 86-87 0.5644834543022803 1106 88-89 0.3664330118490895 1106 90-91 0.38416665838328257 1106 92-93 0.3594237441000345 1106 94-95 0.48868546247300415 1106 96-97 0.5179787863297882 1106 98-99 0.8697230812158949 1106 100-101 1.0754593678843563 1107 1 0.19564980954679712 1107 2 0.2495578783029302 1107 3 0.19239524608792635 1107 4 0.14470485440098457 1107 5 0.13704435907748547 1107 6 0.20623377526533915 1107 7 0.3186090341104375 1107 8 0.14957437084399317 1107 9 0.26875872391857314 1107 10-11 0.2682948822926292 1107 12-13 0.21587691661978425 1107 14-15 0.37654634591036285 1107 16-17 0.3546104276291473 1107 18-19 0.3612860561936486 1107 20-21 0.267054447982197 1107 22-23 0.2735667485764637 1107 24-25 0.24845366282686854 1107 26-27 0.3623402693609066 1107 28-29 0.4181429938635546 1107 30-31 0.5345174162045296 1107 32-33 0.6596301263474373 1107 34-35 0.42969752589557686 1107 36-37 0.5190901981095521 1107 38-39 0.6365405700457316 1107 40-41 0.5988463873947083 1107 42-43 0.7661107663154283 1107 44-45 0.7693736146712524 1107 46-47 0.780741437369052 1107 48-49 0.6656400658447268 1107 50-51 0.6430210499808666 1107 52-53 0.7687820093663333 1107 54-55 0.5742526115985029 1107 56-57 0.783114728354164 1107 58-59 0.7639187542712236 1107 60-61 0.7456869450988393 1107 62-63 0.6346786860098206 1107 64-65 0.6639654037455358 1107 66-67 0.4547832198512438 1107 68-69 0.615671164799501 1107 70-71 0.5867339366103437 1107 72-73 0.4985851183457619 1107 74-75 0.5427808309996358 1107 76-77 0.42997012808045554 1107 78-79 0.6091530678636268 1107 80-81 0.5829191624496985 1107 82-83 0.6637283110918233 1107 84-85 0.624301283662863 1107 86-87 0.5777405386191425 1107 88-89 0.511124252265585 1107 90-91 0.5665712633126994 1107 92-93 0.5335000511779313 1107 94-95 0.550926037891653 1107 96-97 0.34677786656499165 1107 98-99 0.43573802335349754 1107 100-101 0.7193364763894863 1108 1 0.0738222967439981 1108 2 0.11998861849457754 1108 3 0.10926372694356346 1108 4 0.06486288378440719 1108 5 0.04966586851110577 1108 6 8.856765051632465E-4 1108 7 0.02784143031605879 1108 8 0.2555293228226816 1108 9 0.28094311337490296 1108 10-11 0.12609642272418142 1108 12-13 0.31962647408473543 1108 14-15 0.2085177178737041 1108 16-17 0.24956011287181923 1108 18-19 0.17578081994590633 1108 20-21 0.19217319299372804 1108 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-1.4354766925942641 2316 80-81 -1.2907659303796066 2316 82-83 -1.0620570409770025 2316 84-85 -1.083644886716776 2316 86-87 -1.0091174244646695 2316 88-89 -0.8108753346275535 2316 90-91 -0.928312797348152 2316 92-93 -0.7273529845588058 2316 94-95 -1.407680953792326 2316 96-97 -1.2173869091898268 2316 98-99 -1.165007664469428 2316 100-101 -1.6609456037532446 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 2.0 4 19.0 5 30.0 6 79.0 7 216.0 8 581.0 9 1006.0 10 1744.0 11 2912.0 12 4112.0 13 5348.0 14 6541.0 15 8075.0 16 9592.0 17 11185.0 18 9662.0 19 7751.0 20 7501.0 21 7908.0 22 8742.0 23 10147.0 24 12275.0 25 15472.0 26 19597.0 27 24775.0 28 31412.0 29 38429.0 30 47433.0 31 56756.0 32 67974.0 33 81618.0 34 100513.0 35 126012.0 36 165781.0 37 223300.0 38 203813.0 39 34313.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.61075192500342 21.55575024305132 14.806279780717805 23.027218051227454 2 15.376164261622655 22.206326603941818 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27.742562692216705 30.99723503666903 20.30684439318666 22-23 20.720120623864023 27.56661167521603 30.920326222641815 20.79294147827813 24-25 20.69608707464888 27.737430528986994 30.947541183580906 20.61894121278322 26-27 20.66980704177921 27.966687823474594 30.718206474426818 20.645298660319376 28-29 20.689039516529387 28.118308207711383 30.66204408945049 20.530608186308736 30-31 20.90992905284513 27.796150399687704 30.705826597937534 20.588093949529632 32-33 20.731844837447717 28.00333789453096 30.552863738257013 20.71195352976431 34-35 20.83287891631986 28.07498601627916 30.59970845836733 20.49242660903365 36-37 20.93277111809588 27.963542015140185 30.550991386751175 20.552695480012762 38-39 20.76471423206243 28.08900546784205 30.540773718871893 20.605506581223626 40-41 20.910977829940208 28.001774344093377 30.476799526841575 20.610448299124844 42-43 20.79510149398075 28.099570127483002 30.306603947965762 20.798724430570488 44-45 20.816032602266542 28.300003548867913 30.223600858826032 20.66036299003951 46-47 20.94505770752157 28.308034691055887 29.912939645547908 20.833967955874634 48-49 21.022802049522742 28.46422577282238 29.619189506916772 20.89378267073811 50-51 21.021719406743568 28.640620660284778 29.482548558520193 20.85511137445146 52-53 21.135927557604028 28.664297067111722 29.386981758030707 20.812793617253547 54-55 20.984593975786467 28.68997220179596 29.538410803404698 20.787023019012878 56-57 21.030231560891938 28.720833086887087 29.35688620098184 20.89204915123913 58-59 21.045848386662662 28.64773656587406 29.452223949175327 20.85419109828795 60-61 20.971272690533123 28.773866486458683 29.543389624890317 20.71147119811788 62-63 21.026116610324227 28.731976471041566 29.497564257798896 20.74434266083531 64-65 20.804574106543395 28.605432548157655 29.50070474618022 21.08928859911873 66-67 20.77075407862956 28.722201099986734 29.430240667492285 21.07680415389142 68-69 20.866792530436122 28.731585939077736 29.468241013678885 20.933380516807258 70-71 20.875516807378077 28.57267240775644 29.474220856954535 21.07758992791095 72-73 20.922716709139237 28.748307545579184 29.29603892543352 21.03293681984806 74-75 20.949180281694314 28.96391134382213 29.086694902314246 21.000213472169307 76-77 20.920930301470126 28.810343103301395 29.132889097960195 21.135837497268287 78-79 20.844823838540965 28.861623018765815 29.220200714789517 21.073352427903703 80-81 20.898813356704927 28.969665964089458 28.9924732319562 21.139047447249414 82-83 20.96474302105512 28.94724390820913 29.060947480482614 21.027065590253137 84-85 20.9351342559735 29.020951620534657 28.90399515022475 21.13991897326709 86-87 20.920459250059142 29.055402767920512 28.934121421812158 21.090016560208184 88-89 20.88359158784849 28.911346231827643 28.902141998767593 21.30292018155627 90-91 20.920687390000705 29.019075231476776 28.925105660317374 21.135131718205145 92-93 20.954599230996745 28.983288967761016 28.949275362318836 21.112836438923395 94-95 20.81244150163906 28.95208642305719 28.925544986625777 21.30992708867797 96-97 20.871259167258103 28.788110657676842 28.98062307783298 21.360007097232078 98-99 20.73489620298084 28.94776141471493 28.793581440738116 21.523760941566124 100-101 20.71249556422995 28.894753962621245 28.81753459900639 21.575215874142415 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 354.0 1 266.5 2 347.5 3 519.5 4 389.0 5 279.5 6 428.5 7 856.0 8 1387.0 9 1653.0 10 1442.0 11 1158.0 12 1171.0 13 1292.5 14 1433.5 15 1615.5 16 1785.5 17 1981.5 18 2226.0 19 2454.0 20 2846.0 21 3325.0 22 4020.5 23 4966.5 24 6132.5 25 7844.5 26 10061.5 27 12584.0 28 15321.0 29 18434.0 30 21689.0 31 25244.0 32 29395.0 33 32445.0 34 34726.0 35 37504.0 36 41300.5 37 45312.0 38 47980.5 39 49397.0 40 50624.0 41 52403.0 42 53574.5 43 54613.0 44 55621.5 45 56449.5 46 57008.5 47 56379.0 48 55147.0 49 53176.0 50 49830.5 51 46145.0 52 42709.0 53 38839.0 54 34662.0 55 29431.0 56 23839.5 57 18826.5 58 14560.5 59 11204.5 60 8256.5 61 6045.0 62 4223.5 63 2604.0 64 1462.5 65 762.0 66 367.5 67 207.5 68 107.5 69 62.0 70 35.0 71 18.0 72 14.5 73 12.0 74 8.5 75 6.0 76 5.5 77 2.5 78 2.0 79 2.5 80 0.5 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0033268275372604684 2 0.0048793470546486875 3 0.0018482375207002604 4 0.16759817837709962 5 0.001996096522356281 6 4.435770049680625E-4 7 1.4785900165602083E-4 8 6.653655074520937E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 4.066122545540573E-4 24-25 5.544712562100781E-4 26-27 0.0030680742843624317 28-29 2.5875325289803646E-4 30-31 0.006210078069552875 32-33 0.021476519990537023 34-35 0.012900697894487816 36-37 0.0010719777620061509 38-39 2.9571800331204166E-4 40-41 0.002033061272770286 42-43 0.010793707120889518 44-45 0.007097232079489 46-47 0.009610835107641352 48-49 0.009610835107641352 50-51 0.18223621954104566 52-53 0.1107094274899456 54-55 0.015857877927608233 56-57 0.005914360066240833 58-59 0.016708067187130353 60-61 0.11891560208185474 62-63 0.23317364561154486 64-65 0.23790513366453747 66-67 0.2477377572746629 68-69 0.24378252898036432 70-71 0.23128844334043058 72-73 0.1597246865389165 74-75 0.26008398391294063 76-77 0.2036757747811687 78-79 0.005470783061272771 80-81 7.392950082801041E-5 82-83 0.0 84-85 0.0 86-87 0.0 88-89 1.1089425124201562E-4 90-91 0.005507747811686775 92-93 0.017743080198722498 94-95 0.0028093210314643954 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1352640.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.64434887339628 #Duplication Level Percentage of deduplicated Percentage of total 1 80.75623084353651 54.627026528807086 2 12.200970789596642 16.50653449371185 3 3.1672071610558836 6.427309984703494 4 1.3442069305833848 3.637120102816786 5 0.7130530509016823 2.411700467021649 6 0.4478760368709686 1.8177769734080336 7 0.2958510040372129 1.4008853972146458 8 0.20527709651574147 1.1108668425942925 9 0.15082211004367324 0.9182037086654398 >10 0.6818764771680672 7.716218935523159 >50 0.025227932371324816 1.1598546713176803 >100 0.010633241656907393 1.288681232930535 >500 4.3847180684950736E-4 0.20576722037363074 >1k 3.2885385513713053E-4 0.7720534409117994 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4234 0.3130175065057961 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3734 0.27605275609179086 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2444 0.18068370002365744 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 2.2178850248403122E-4 0.0 0.0 0.0 0.0 7 2.2178850248403122E-4 0.0 0.0 0.0 0.0 8 2.2178850248403122E-4 0.0 0.0 0.0 0.0 9 2.2178850248403122E-4 0.0 0.0 0.0 0.0 10-11 2.587532528980364E-4 0.0 0.0 0.0 0.0 12-13 2.9571800331204166E-4 0.0 0.0 0.0 0.0 14-15 3.3268275372604685E-4 3.696475041400521E-5 0.0 0.0 0.0 16-17 4.4357700496806243E-4 7.392950082801041E-5 0.0 0.0 0.0 18-19 4.4357700496806243E-4 7.392950082801041E-5 0.0 0.0 0.0 20-21 4.4357700496806243E-4 7.392950082801041E-5 0.0 0.0 0.0 22-23 5.544712562100781E-4 7.392950082801041E-5 0.0 3.696475041400521E-5 0.0 24-25 5.914360066240833E-4 7.392950082801041E-5 0.0 1.4785900165602083E-4 7.392950082801041E-5 26-27 5.914360066240833E-4 7.392950082801041E-5 0.0 2.9571800331204166E-4 7.392950082801041E-5 28-29 5.914360066240833E-4 7.392950082801041E-5 0.0 5.175065057960728E-4 7.392950082801041E-5 30-31 5.914360066240833E-4 7.392950082801041E-5 0.0 0.0018852022711142655 7.392950082801041E-5 32-33 5.914360066240833E-4 7.392950082801041E-5 0.0 0.005877395315826827 7.392950082801041E-5 34-35 5.914360066240833E-4 1.4785900165602083E-4 0.0 0.01164389638041164 7.392950082801041E-5 36-37 5.914360066240833E-4 1.4785900165602083E-4 0.0 0.021365625739295006 7.392950082801041E-5 38-39 5.914360066240833E-4 1.8482375207002602E-4 0.0 0.03814762242725337 1.1089425124201562E-4 40-41 5.914360066240833E-4 2.2178850248403122E-4 0.0 0.05640820913177194 1.4785900165602083E-4 42-43 5.914360066240833E-4 2.2178850248403122E-4 0.0 0.07481665483794653 1.4785900165602083E-4 44-45 5.914360066240833E-4 2.2178850248403122E-4 0.0 0.09884374260704992 1.4785900165602083E-4 46-47 5.914360066240833E-4 2.2178850248403122E-4 0.0 0.12512568015140763 1.4785900165602083E-4 48-49 5.914360066240833E-4 2.2178850248403122E-4 0.0 0.15787644901821624 1.8482375207002602E-4 50-51 6.653655074520937E-4 2.2178850248403122E-4 0.0 0.191736160397445 2.2178850248403122E-4 52-53 6.653655074520937E-4 2.2178850248403122E-4 0.0 0.2263721315353679 2.9571800331204166E-4 54-55 6.653655074520937E-4 2.2178850248403122E-4 0.0 0.2621170451857109 3.6964750414005204E-4 56-57 6.653655074520937E-4 2.2178850248403122E-4 0.0 0.3076945824461793 3.6964750414005204E-4 58-59 6.653655074520937E-4 2.587532528980364E-4 0.0 0.3572643127513603 3.6964750414005204E-4 60-61 7.762597586941093E-4 2.9571800331204166E-4 0.0 0.41230482611781405 3.6964750414005204E-4 62-63 8.132245091081145E-4 2.9571800331204166E-4 0.0 0.4724834397918145 3.6964750414005204E-4 64-65 8.132245091081145E-4 2.9571800331204166E-4 0.0 0.5353235154956234 3.6964750414005204E-4 66-67 8.132245091081145E-4 2.9571800331204166E-4 0.0 0.6062219067896853 3.6964750414005204E-4 68-69 8.132245091081145E-4 2.9571800331204166E-4 0.0 0.6903167139815471 3.6964750414005204E-4 70-71 8.132245091081145E-4 2.9571800331204166E-4 0.0 0.7842811095339484 3.6964750414005204E-4 72-73 8.132245091081145E-4 2.9571800331204166E-4 0.0 0.8839011118996924 3.6964750414005204E-4 74-75 8.132245091081145E-4 2.9571800331204166E-4 0.0 0.9896942275845753 3.6964750414005204E-4 76-77 8.132245091081145E-4 2.9571800331204166E-4 0.0 1.1028063638514314 3.6964750414005204E-4 78-79 8.132245091081145E-4 2.9571800331204166E-4 0.0 1.2327004968062456 3.6964750414005204E-4 80-81 8.132245091081145E-4 2.9571800331204166E-4 0.0 1.3759019399101016 3.6964750414005204E-4 82-83 8.132245091081145E-4 2.9571800331204166E-4 0.0 1.526681156848829 3.6964750414005204E-4 84-85 8.132245091081145E-4 2.9571800331204166E-4 0.0 1.6861470901348474 3.6964750414005204E-4 86-87 8.132245091081145E-4 2.9571800331204166E-4 0.0 1.8647607641353205 3.6964750414005204E-4 88-89 8.132245091081145E-4 2.9571800331204166E-4 0.0 2.059823752070026 3.6964750414005204E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1870 0.0 54.13764 1 GTATCAA 3600 0.0 39.739773 1 TAACGCA 140 4.5474735E-10 33.94321 4 ATCAACG 4325 0.0 32.412823 3 TCAACGC 4375 0.0 31.933775 4 CAACGCA 4370 0.0 31.918308 5 TATCAAC 4470 0.0 31.79135 2 AACGCAG 4530 0.0 30.89454 6 ACGCAGA 5295 0.0 26.341425 7 CGCAGAG 5370 0.0 25.79684 8 ATAACGC 170 1.1184602E-7 25.157907 3 GCGGTAT 95 0.0012222653 25.015417 1 TAGCCCG 125 2.2745406E-4 22.772738 5 CCTATAC 420 0.0 22.628807 3 GTACATG 2900 0.0 22.453493 1 GCAGAGT 6100 0.0 22.24304 9 TATAACG 195 4.1990825E-7 21.935785 2 GTATAAC 535 0.0 21.32155 1 TGGTATC 990 0.0 20.643272 2 TACATGG 2900 0.0 20.322117 2 >>END_MODULE