FastQCFastQC Report
Sat 18 Jun 2016
SRR1931015_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931015_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1393517
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN591984.248100310222265No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15640.11223400934470122No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11150.053.5919461
GTATCAA23300.044.4967881
ATCAACG25850.039.054233
TCAACGC26800.037.8512464
CAACGCA27550.036.9991765
TATCAAC28550.035.0527152
AACGCAG29850.034.340936
ACGCAGA33650.030.560697
CGCAGAG33800.030.2824548
GCAGAGT38800.026.371659
GTACATG22500.023.1529431
GTCTTAC3750.023.1529431
ACATGGG21900.022.705113
TACATGG22100.022.6415142
CGATACA1150.002695910921.8341244
AGAGTAC34750.021.3238310-11
GAGTACT26600.021.2945812-13
GTACTAG2458.1688995E-820.8459851
CAGAGTA38050.020.62001210-11
TAACGCA1250.00437119720.0873934
Produced by FastQC (version 0.11.5)