FastQCFastQC Report
Sat 18 Jun 2016
SRR1931015_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931015_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1393517
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40000.2870435021603611No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35620.2556122386738016No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23060.1654805789954482No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20550.055.7188721
GTATCAA36300.038.480241
TATCACG1201.5035403E-731.6730752
TCAACGC45300.030.409084
ATCAACG45850.030.0464613
CAACGCA46250.029.7513285
TATCAAC46900.029.2756442
AACGCAG48250.028.712736
ATCACGC1405.8027945E-727.1454243
GTGGTAT10000.026.6063371
TGGTATC10300.024.9079512
CGCAGAG54950.024.8664468
ACGCAGA55550.024.6832717
CAGTGCG2151.6862032E-924.2741019
GTACATG31150.023.793791
GTATACG1801.9471554E-723.755661
GGGACGC1201.724604E-423.7224186
TACATGG31950.022.3049812
CGTATAC1302.9400608E-421.925153
ACATGGG31000.021.30043
Produced by FastQC (version 0.11.5)