##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931015_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1393517 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.279698058940077 31.0 31.0 34.0 30.0 34.0 2 31.43626880762847 31.0 31.0 34.0 30.0 34.0 3 31.49874095543865 31.0 31.0 34.0 30.0 34.0 4 35.28358462795933 37.0 35.0 37.0 33.0 37.0 5 35.1535230643042 37.0 35.0 37.0 32.0 37.0 6 35.18598266113725 37.0 35.0 37.0 32.0 37.0 7 35.06696007296646 37.0 35.0 37.0 32.0 37.0 8 35.07061772479274 37.0 35.0 37.0 32.0 37.0 9 36.569835890053724 39.0 35.0 39.0 32.0 39.0 10-11 36.50380332640363 38.0 35.0 39.0 32.0 39.0 12-13 36.38665764393258 38.0 35.0 39.0 32.0 39.0 14-15 37.4310517919767 39.0 36.0 41.0 32.0 41.0 16-17 37.381482608393014 39.0 36.0 41.0 32.0 41.0 18-19 37.40230546164848 39.0 36.0 41.0 32.0 41.0 20-21 37.38901606510721 39.0 36.0 41.0 32.0 41.0 22-23 37.28390791070363 39.0 36.0 40.0 31.0 41.0 24-25 37.15570925937753 39.0 36.0 40.0 31.0 41.0 26-27 36.88527050620839 39.0 36.0 40.0 31.0 41.0 28-29 36.688421454492484 39.0 35.5 40.0 30.0 41.0 30-31 36.44827224928006 38.0 35.0 40.0 30.0 41.0 32-33 36.27309534078164 38.0 35.0 40.0 30.0 41.0 34-35 36.33005051248029 38.0 35.0 40.0 30.0 41.0 36-37 36.39445051621186 38.0 35.0 40.0 30.0 41.0 38-39 36.38182239613869 38.0 35.0 40.0 30.0 41.0 40-41 36.256450764504486 38.0 35.0 40.0 30.0 41.0 42-43 36.10896278983321 38.0 35.0 40.0 30.0 41.0 44-45 35.93531151754877 38.0 35.0 40.0 29.0 41.0 46-47 35.685819046341024 38.0 35.0 40.0 28.0 41.0 48-49 35.464497383239674 38.0 34.0 40.0 27.0 41.0 50-51 35.19896563873996 38.0 34.0 40.0 26.5 41.0 52-53 34.9834221613371 38.0 34.0 40.0 26.0 41.0 54-55 34.823034092874366 38.0 33.5 40.0 26.0 41.0 56-57 34.651212364111814 38.0 33.0 40.0 26.0 41.0 58-59 34.43495486599733 38.0 33.0 40.0 25.5 41.0 60-61 34.120611373955256 37.0 33.0 40.0 24.5 41.0 62-63 33.85968129559955 37.0 32.5 40.0 23.5 41.0 64-65 33.53906949107905 36.5 32.0 39.5 23.0 41.0 66-67 33.2179933936938 36.0 32.0 39.0 22.5 40.5 68-69 32.8639959182414 36.0 31.0 39.0 22.0 40.0 70-71 32.474066695992946 35.0 31.0 38.5 21.5 40.0 72-73 32.03089054528937 35.0 31.0 38.0 20.5 40.0 74-75 31.50542691621272 35.0 30.0 37.0 19.0 39.0 76-77 30.155961498855056 33.5 28.5 36.0 18.0 38.5 78-79 30.878380026939034 34.0 30.0 36.0 19.0 39.0 80-81 30.833150582303624 34.0 30.0 36.0 18.5 38.5 82-83 30.553615061746644 34.0 30.0 36.0 18.0 37.0 84-85 30.194942006448432 34.0 30.0 35.0 17.5 37.0 86-87 29.827243585833543 34.0 29.0 35.0 12.0 36.5 88-89 29.458757230805222 34.0 29.0 35.0 7.5 36.0 90-91 29.104926240584078 34.0 29.0 35.0 4.5 36.0 92-93 28.754709128055133 34.0 29.0 35.0 2.0 35.0 94-95 28.345247671897795 34.0 29.0 35.0 2.0 35.0 96-97 27.882040190395955 33.0 28.0 35.0 2.0 35.0 98-99 27.348023741368063 33.0 27.0 35.0 2.0 35.0 100-101 26.185764508075607 32.0 25.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.19594759985638888 1101 2 0.31168703008109233 1101 3 0.2527116927639703 1101 4 0.07424432246315149 1101 5 0.2550198277760103 1101 6 0.20472821429501664 1101 7 0.28720358312045136 1101 8 0.267090074189845 1101 9 0.31570327954247546 1101 10-11 0.30002292596719826 1101 12-13 0.37045967203300023 1101 14-15 0.22797090658290387 1101 16-17 0.4199172071405002 1101 18-19 0.4948855786182662 1101 20-21 0.4108637609974224 1101 22-23 0.5069426274470743 1101 24-25 0.3538703440402813 1101 26-27 0.2964641068039171 1101 28-29 0.4998991350293238 1101 30-31 0.5545537040617816 1101 32-33 0.4575152399142297 1101 34-35 0.4419260805640093 1101 36-37 0.48696556713663597 1101 38-39 0.5291924485178754 1101 40-41 0.6563668736871335 1101 42-43 0.63612941303208 1101 44-45 0.5603597555391815 1101 46-47 0.5288362359301075 1101 48-49 0.4636035303509587 1101 50-51 0.4081059864032568 1101 52-53 0.24267541828617567 1101 54-55 0.4151723649738983 1101 56-57 0.545898379784667 1101 58-59 0.46585676181395996 1101 60-61 0.44891246661510564 1101 62-63 -0.06239733136017378 1101 64-65 0.16258468452902264 1101 66-67 0.03020462776882482 1101 68-69 0.26181561371577544 1101 70-71 0.2810150125254083 1101 72-73 0.3484826579912301 1101 74-75 0.17314677312540283 1101 76-77 0.10674175838412481 1101 78-79 0.39969389934181265 1101 80-81 0.329038871101492 1101 82-83 0.3354424183448401 1101 84-85 0.20662732814204787 1101 86-87 0.27215277794303105 1101 88-89 0.2574703391723254 1101 90-91 0.19059394591215906 1101 92-93 0.24282681968222164 1101 94-95 0.33874296241954127 1101 96-97 0.4122362733340559 1101 98-99 0.31994611489139047 1101 100-101 0.5543748053757547 1102 1 0.304672432071154 1102 2 0.4841702515576003 1102 3 0.4916378672606143 1102 4 0.08968056407388758 1102 5 0.236899022406881 1102 6 0.3611040532211902 1102 7 0.44492170392582153 1102 8 0.39863369835091333 1102 9 0.48684421913979037 1102 10-11 0.5178081608665366 1102 12-13 0.3832113498853502 1102 14-15 0.37427963141511356 1102 16-17 0.48971586485861707 1102 18-19 0.5116641021081918 1102 20-21 0.37428657978937707 1102 22-23 0.41835202342022626 1102 24-25 0.34682336417450443 1102 26-27 0.4350547108307623 1102 28-29 0.4646642357004538 1102 30-31 0.4263657845986941 1102 32-33 0.54845483722967 1102 34-35 0.49964420136937804 1102 36-37 0.53126086915006 1102 38-39 0.3775145961688793 1102 40-41 0.5328769408012306 1102 42-43 0.5109616277971867 1102 44-45 0.5952590843982435 1102 46-47 0.6607154305609768 1102 48-49 0.5169592350489367 1102 50-51 0.3319314897589649 1102 52-53 0.2728767605680531 1102 54-55 0.5175213582624849 1102 56-57 0.3331467019323142 1102 58-59 0.3416956879884552 1102 60-61 0.14455005050772485 1102 62-63 -0.03186041860849542 1102 64-65 0.050839718086074015 1102 66-67 0.044634157970165234 1102 68-69 0.079936419084909 1102 70-71 0.01927004608246108 1102 72-73 -0.02030928831750245 1102 74-75 0.05670382010526964 1102 76-77 0.20137263086734336 1102 78-79 0.36949255705993167 1102 80-81 0.2112536362021622 1102 82-83 0.16899946532470622 1102 84-85 0.09387565028970002 1102 86-87 0.1224883484128263 1102 88-89 0.2765978559508504 1102 90-91 0.36576173114706023 1102 92-93 0.3347731284070541 1102 94-95 0.2766624255067889 1102 96-97 0.35820942769647246 1102 98-99 0.5256508128779664 1102 100-101 0.8422942684630037 1103 1 0.28811388119687464 1103 2 0.31529862129116637 1103 3 0.2937388302019599 1103 4 0.2677641087754665 1103 5 0.3225985577708457 1103 6 0.3212396281802299 1103 7 0.44997209561174856 1103 8 0.36637628549823376 1103 9 0.5449843282282458 1103 10-11 0.4800839795911429 1103 12-13 0.43898675335552895 1103 14-15 0.46399478932402616 1103 16-17 0.43340960910678206 1103 18-19 0.4043702142044481 1103 20-21 0.5353918299521112 1103 22-23 0.32685014916376076 1103 24-25 0.5162034005373286 1103 26-27 0.4816226891028563 1103 28-29 0.40980014735227144 1103 30-31 0.4209360523816841 1103 32-33 0.4819905603335286 1103 34-35 0.5635440015983306 1103 36-37 0.41931837627672763 1103 38-39 0.5212790459006413 1103 40-41 0.587591762017361 1103 42-43 0.4659413139995152 1103 44-45 0.4124416392230543 1103 46-47 0.3901322786676431 1103 48-49 0.3787737846662296 1103 50-51 0.45642701174500644 1103 52-53 0.373166316358045 1103 54-55 0.4274807306671491 1103 56-57 0.4841803487640348 1103 58-59 0.5566583150899831 1103 60-61 0.4892897869895094 1103 62-63 0.3731866061496447 1103 64-65 0.30647932213536677 1103 66-67 0.22715980273683556 1103 68-69 0.23341445549261408 1103 70-71 0.5076945483383 1103 72-73 0.41659448488625017 1103 74-75 0.4740513543918219 1103 76-77 0.38830782873046843 1103 78-79 0.5283576779326431 1103 80-81 0.5359915769891224 1103 82-83 0.6432559354376117 1103 84-85 0.6227032860330048 1103 86-87 0.6809427040950773 1103 88-89 0.46121324056623436 1103 90-91 0.557302886227756 1103 92-93 0.6202583027016821 1103 94-95 0.8098434988833674 1103 96-97 1.0565933023569762 1103 98-99 0.8908917726768486 1103 100-101 1.0163588236918422 1104 1 0.3122183522407056 1104 2 0.4487896439984347 1104 3 0.3921942077763312 1104 4 0.25244990352990726 1104 5 0.2867179909692297 1104 6 0.09427254492023707 1104 7 0.35433092328293725 1104 8 0.3089304132926358 1104 9 0.4431052576420811 1104 10-11 0.46722532794530025 1104 12-13 0.43176751378502587 1104 14-15 0.6109539514433706 1104 16-17 0.5403296055862796 1104 18-19 0.5038179346761922 1104 20-21 0.5184405889734123 1104 22-23 0.44383109441987756 1104 24-25 0.47138844718404727 1104 26-27 0.6095381089233101 1104 28-29 0.6323412932772854 1104 30-31 0.621954799081216 1104 32-33 0.6680702558096812 1104 34-35 0.6040397329836509 1104 36-37 0.6295344473909594 1104 38-39 0.5680192764938568 1104 40-41 0.6590558528464356 1104 42-43 0.6531772759780381 1104 44-45 0.8144219243841135 1104 46-47 0.5882609091904314 1104 48-49 0.6297727285141477 1104 50-51 0.6074083530581476 1104 52-53 0.4571513101971547 1104 54-55 0.443728970413666 1104 56-57 0.6540492098800357 1104 58-59 0.461573822923107 1104 60-61 0.4914681006666797 1104 62-63 0.4966078788129096 1104 64-65 0.38609448671906677 1104 66-67 0.3434861537313765 1104 68-69 0.45111930499094655 1104 70-71 0.28385411531593974 1104 72-73 0.1810241627598579 1104 74-75 0.49460341388838813 1104 76-77 0.6124310024674848 1104 78-79 0.6820058032626868 1104 80-81 0.4496742472936468 1104 82-83 0.4239866606620417 1104 84-85 0.5419571585906517 1104 86-87 0.6184328026692718 1104 88-89 0.6223215401613729 1104 90-91 0.5138697318535215 1104 92-93 0.4376034003957514 1104 94-95 0.6218165229988415 1104 96-97 0.685843214344299 1104 98-99 0.9965135822174211 1104 100-101 1.2093068082016138 1105 1 0.3541930772866735 1105 2 0.37797336880154475 1105 3 0.38732743471315345 1105 4 0.2943400606960651 1105 5 0.2209809927386459 1105 6 0.2800061604356259 1105 7 0.19098090767040787 1105 8 0.4052163654835894 1105 9 0.4535977462796339 1105 10-11 0.5244348481201655 1105 12-13 0.4464775543776156 1105 14-15 0.5496721625941845 1105 16-17 0.5203286514871266 1105 18-19 0.5615760618662833 1105 20-21 0.5017108939381885 1105 22-23 0.5229699331944602 1105 24-25 0.6973210791099405 1105 26-27 0.45419992414032606 1105 28-29 0.44929325004792275 1105 30-31 0.4891645794649335 1105 32-33 0.6144626767929253 1105 34-35 0.6054867110914088 1105 36-37 0.6532043464299235 1105 38-39 0.6940672676814472 1105 40-41 0.6248431581234684 1105 42-43 0.5579959104019423 1105 44-45 0.5853808807731085 1105 46-47 0.5761948074661163 1105 48-49 0.42152670970971684 1105 50-51 0.346534224757832 1105 52-53 0.31357391540827706 1105 54-55 0.4722359353375154 1105 56-57 0.5912842305232786 1105 58-59 0.3620604134931469 1105 60-61 0.36668841083564274 1105 62-63 0.4112144863832583 1105 64-65 0.3793028186067886 1105 66-67 0.25322316930921573 1105 68-69 0.2703966616158695 1105 70-71 0.20552647766545107 1105 72-73 0.2874851487779644 1105 74-75 0.2970773496341437 1105 76-77 0.4058735223300971 1105 78-79 0.4691089847496954 1105 80-81 0.5193679380961314 1105 82-83 0.3652239104278543 1105 84-85 0.5466940440640577 1105 86-87 0.5507032550857467 1105 88-89 0.7127217847577043 1105 90-91 0.7299092228849915 1105 92-93 0.6336668740105722 1105 94-95 0.5531098911423378 1105 96-97 0.6509273051745375 1105 98-99 0.7745567248465726 1105 100-101 0.9040761764144989 1106 1 0.2546558196716582 1106 2 0.2153151781307976 1106 3 0.22411621576660323 1106 4 0.104977261528866 1106 5 0.1468686770904739 1106 6 0.1663934412203929 1106 7 0.19855317445541942 1106 8 0.2368355722295803 1106 9 0.3842681897247644 1106 10-11 0.22936706843513832 1106 12-13 0.3524662283933324 1106 14-15 0.3430062356191641 1106 16-17 0.47196717723817727 1106 18-19 0.3116497046952986 1106 20-21 0.32693459626887744 1106 22-23 0.2612718187554677 1106 24-25 0.3614212333573974 1106 26-27 0.3859429204570972 1106 28-29 0.4137671661277267 1106 30-31 0.18076364049166926 1106 32-33 0.2726657482536581 1106 34-35 0.37334123550232334 1106 36-37 0.20897035150769483 1106 38-39 0.19476823428436774 1106 40-41 0.1487140949864454 1106 42-43 0.15318813232683226 1106 44-45 0.10181987470546261 1106 46-47 0.1137787570278519 1106 48-49 0.1262422438867361 1106 50-51 0.012528207148825743 1106 52-53 0.06440443682561181 1106 54-55 -0.1250883389488422 1106 56-57 0.13610149405582206 1106 58-59 0.0069403332583561905 1106 60-61 0.1956586513006897 1106 62-63 -0.010918827805120657 1106 64-65 -0.3086282421327766 1106 66-67 -0.23663370035929177 1106 68-69 -0.41692937487703574 1106 70-71 -0.24609420846380914 1106 72-73 -0.22073456574456785 1106 74-75 -0.08245603547760183 1106 76-77 -0.04295700343836728 1106 78-79 -0.057889107280995944 1106 80-81 -0.17296015430440903 1106 82-83 -0.18607174649445923 1106 84-85 -0.2526678631220456 1106 86-87 -0.2293622728909135 1106 88-89 -0.21680148398314714 1106 90-91 -0.3647530317386263 1106 92-93 -0.4015293944626812 1106 94-95 -0.3150679220246069 1106 96-97 -0.01907145475418659 1106 98-99 0.004975020466513769 1106 100-101 0.03281080333796993 1107 1 0.050551075201187956 1107 2 0.06670190726096692 1107 3 0.12287416313191812 1107 4 0.05276380956736659 1107 5 0.10956904255445465 1107 6 0.12129636690063705 1107 7 0.17964090549492795 1107 8 0.09945917057351039 1107 9 0.21692636607917848 1107 10-11 0.2029565546203429 1107 12-13 0.2597919112598319 1107 14-15 0.16571224379452332 1107 16-17 0.2538803188505554 1107 18-19 0.25438340152422256 1107 20-21 0.13779812795007018 1107 22-23 0.10422203823069509 1107 24-25 0.03691480657158053 1107 26-27 0.17080864360993075 1107 28-29 0.2105337612763094 1107 30-31 0.23707068272670284 1107 32-33 0.23388654230125638 1107 34-35 0.025432647426811172 1107 36-37 0.13726133169915045 1107 38-39 0.21444358154076326 1107 40-41 0.2087781331994023 1107 42-43 0.2553881336390944 1107 44-45 0.2242942692622094 1107 46-47 0.25761212644829357 1107 48-49 0.12701122734571868 1107 50-51 0.07975061527432814 1107 52-53 0.2913956250100327 1107 54-55 0.374962839131193 1107 56-57 0.3577471973935573 1107 58-59 0.26959673806384643 1107 60-61 0.18150594378341367 1107 62-63 -0.047783448660844385 1107 64-65 -0.19648394619223808 1107 66-67 -0.09769308661600462 1107 68-69 -0.04252300779898377 1107 70-71 -0.059100002084917946 1107 72-73 0.009280065937286253 1107 74-75 -0.04882875624868532 1107 76-77 -0.06027159772085966 1107 78-79 0.012443353400460211 1107 80-81 0.1581961422397633 1107 82-83 0.19865553339058195 1107 84-85 0.2662877103677097 1107 86-87 0.24474096248232513 1107 88-89 0.33736657695813577 1107 90-91 0.4226505992805407 1107 92-93 0.16627828093706398 1107 94-95 0.45824074084962874 1107 96-97 0.5238945118225864 1107 98-99 0.465633511762956 1107 100-101 0.3319302779096809 1108 1 0.0565535648524893 1108 2 0.01599873876355673 1108 3 -0.013418001053331352 1108 4 0.10469266624596685 1108 5 0.027614830953673675 1108 6 0.04311846765185123 1108 7 0.08863556080017077 1108 8 0.11887880677466 1108 9 0.09666027513881659 1108 10-11 0.10140757671699419 1108 12-13 0.11338136624694073 1108 14-15 0.06083112086120934 1108 16-17 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-2.5084354802689326 2316 76-77 -1.5604586635142859 2316 78-79 -1.2542607264248922 2316 80-81 -0.8166265930203487 2316 82-83 -0.45424189684282723 2316 84-85 -0.707682283482999 2316 86-87 -0.5504100533446348 2316 88-89 -0.7041829248299329 2316 90-91 -0.4022717385642629 2316 92-93 -0.5314860838253104 2316 94-95 -1.024919357618618 2316 96-97 -0.7734604972620396 2316 98-99 -0.5580929980470515 2316 100-101 -1.1369282426386995 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 3.0 4 21.0 5 42.0 6 72.0 7 229.0 8 555.0 9 1035.0 10 1735.0 11 2780.0 12 3958.0 13 5236.0 14 6247.0 15 7891.0 16 9404.0 17 10766.0 18 9506.0 19 7654.0 20 7584.0 21 8008.0 22 8830.0 23 10450.0 24 12752.0 25 15729.0 26 20261.0 27 25521.0 28 32740.0 29 40435.0 30 48850.0 31 58179.0 32 70463.0 33 85544.0 34 104359.0 35 129856.0 36 168895.0 37 228893.0 38 212774.0 39 36250.0 40 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.904080421454815 21.97000414077486 15.180425114768356 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30.55682133766578 20.19365389873249 20-21 20.827732995004723 28.075079098425064 30.849533948993805 20.247653957576407 22-23 20.54752250337008 27.896213420250326 30.8360844728092 20.7201796035704 24-25 20.545471898638617 28.03770912136197 30.81386629006343 20.602952689935982 26-27 20.551188787200957 28.284818070455316 30.56152981135704 20.60246333098669 28-29 20.546259312787672 28.424364591959606 30.59431049849517 20.43506559675755 30-31 20.763909670835496 28.168370726484227 30.63504126846065 20.43267833421963 32-33 20.625807470356865 28.340702247997474 30.42702621228216 20.606464069363497 34-35 20.740159136116176 28.35942958461907 30.499474103688595 20.40093717557616 36-37 20.74821376736583 28.212662239223313 30.557432317274674 20.48169167613619 38-39 20.61601213911762 28.3865256942623 30.515715155562045 20.481747011058037 40-41 20.820220288390708 28.2345461023623 30.506788164836312 20.43844544441068 42-43 20.739346302407263 28.389023415240505 30.16375453398461 20.70787574836762 44-45 20.63079692151352 28.550780386561907 30.287273254428698 20.531149437495873 46-47 20.838043113959827 28.548052680645654 29.798575690570463 20.815328514824056 48-49 20.772028084448557 28.663943291403786 29.703868824410474 20.860159799737183 50-51 20.949064713238517 28.83797552894862 29.495995838936356 20.716963918876512 52-53 20.988800206898038 28.887994885020724 29.392344772591755 20.73086013548948 54-55 20.836664795784344 28.789577415053092 29.600432647518595 20.773325141643976 56-57 20.83067500776865 28.924244718573927 29.45574237793172 20.789337895725698 58-59 20.901480648644615 28.85920900727028 29.5188421233576 20.720468220727504 60-61 20.918175087588338 28.899787014761685 29.60721125125325 20.574826646396726 62-63 20.912775060443263 28.895960631594768 29.54993100675476 20.64133330120721 64-65 20.68825133408666 28.714853227090714 29.625319270907884 20.97157616791474 66-67 20.68889861608111 28.84710352470045 29.507357677769935 20.956640181448503 68-69 20.73856627106611 28.93756109701649 29.49567942040286 20.828193211514545 70-71 20.756606651855357 28.699369020964788 29.52364067639111 21.020383650788744 72-73 20.78466533583209 28.89106502103162 29.335307424583547 20.988962218552746 74-75 20.728429769606972 29.027219437264712 29.297093511618577 20.94725728150974 76-77 20.76714773538656 28.930527502601166 29.245759220204164 21.05656554180811 78-79 20.67155553912934 29.014454551750298 29.237428472700405 21.076561436419958 80-81 20.78131862138648 29.070853868918622 29.10576555992181 21.042061949773093 82-83 20.907423447291997 29.014141915742687 29.20032550733145 20.878109129633867 84-85 20.867380878740622 29.10796926051135 28.960715943903086 21.06393391684493 86-87 20.804087786514266 29.194907561228174 29.028458210412932 20.972546441844628 88-89 20.817744761360746 29.01704358509109 28.97409465484955 21.191116998698615 90-91 20.824640835685422 29.093439774716295 29.048909786765588 21.033009602832696 92-93 20.838315617217052 29.04282730554738 29.043329720728934 21.075527356506633 94-95 20.705722617376143 28.974663782280256 29.048220659148555 21.271392941195046 96-97 20.74452625981599 28.861004207340134 29.075784507831624 21.318685025012254 98-99 20.635736772497214 28.980234902049993 28.906177678492618 21.47785064696017 100-101 20.622999217088847 28.84916366287602 29.021389764172234 21.5064473558629 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 304.0 1 230.0 2 233.0 3 326.5 4 263.5 5 223.0 6 364.0 7 740.0 8 1210.0 9 1425.0 10 1255.0 11 966.0 12 951.0 13 1124.0 14 1214.5 15 1330.5 16 1564.5 17 1765.0 18 1994.0 19 2262.0 20 2596.0 21 3093.5 22 3730.5 23 4652.0 24 5926.5 25 7707.0 26 10072.5 27 12740.5 28 15647.0 29 19075.0 30 22798.5 31 26610.5 32 31430.0 33 35550.5 34 38241.5 35 41206.0 36 45200.0 37 49352.0 38 51973.5 39 53886.0 40 55177.0 41 56417.0 42 57526.5 43 58261.5 44 58816.0 45 59521.5 46 59799.0 47 58694.0 48 56780.0 49 53814.0 50 49805.5 51 45322.0 52 41282.0 53 37250.0 54 32747.5 55 27450.5 56 22063.0 57 17336.5 58 13319.5 59 9997.0 60 7200.0 61 5211.5 62 3719.5 63 2295.5 64 1220.5 65 639.5 66 318.0 67 175.0 68 98.0 69 57.0 70 30.0 71 21.5 72 19.0 73 16.5 74 9.0 75 5.0 76 7.5 77 4.5 78 0.5 79 1.5 80 1.5 81 1.0 82 0.5 83 0.5 84 0.5 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.004162130781325236 2 0.007176087554009029 3 0.0012916957597216253 4 0.16232310047168422 5 0.0021528262662027084 6 7.176087554009029E-4 7 0.0 8 7.893696309409932E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 2.51163064390316E-4 24-25 5.740870043207223E-4 26-27 0.002726913270523431 28-29 2.1528262662027088E-4 30-31 0.005740870043207223 32-33 0.019877762524605012 34-35 0.013024598910526387 36-37 8.252500687110383E-4 38-39 3.946848154704966E-4 40-41 0.0018299023262723026 42-43 0.010835892206553633 44-45 0.0075348919317094806 46-47 0.010046522575612641 48-49 0.010620609579933363 50-51 0.1813038520520381 52-53 0.10957885694971786 54-55 0.01686380575192122 56-57 0.006889044051848668 58-59 0.017402012318471896 60-61 0.11743667282135775 62-63 0.22701552977107564 64-65 0.23250523674989257 66-67 0.24187003100787433 68-69 0.23752849803769885 70-71 0.22518562744480333 72-73 0.15496761072882498 74-75 0.2590208802619559 76-77 0.20049988625901227 78-79 0.005776750480977268 80-81 7.176087554009029E-5 82-83 0.0 84-85 0.0 86-87 0.0 88-89 1.0764131331013544E-4 90-91 0.0053103047899666815 92-93 0.0177249362584023 94-95 0.002691032832753386 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1393517.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.15885254216192 #Duplication Level Percentage of deduplicated Percentage of total 1 80.57047331349911 54.91591009826977 2 12.657493005403477 17.25440398617483 3 3.184882673859985 6.512338455951274 4 1.2841652157974333 3.501089103332432 5 0.6451074233199744 2.198489086996008 6 0.3908181301349663 1.5982629181603571 7 0.26949179708726956 1.2857776161295398 8 0.18138677319230234 0.9890491461690163 9 0.13372465159920258 0.8203066928641823 >10 0.645849363423701 7.508254748630259 >50 0.024557751662393518 1.1424353254250337 >100 0.011521412938855955 1.4524008023815174 >500 2.1139523253888414E-4 0.11036877665774117 >1k 3.170928488083262E-4 0.7109132428580873 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4000 0.2870435021603611 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3562 0.2556122386738016 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2306 0.1654805789954482 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 7.176087554009029E-5 0.0 6 0.0 0.0 0.0 7.176087554009029E-5 0.0 7 0.0 0.0 0.0 7.176087554009029E-5 0.0 8 0.0 0.0 0.0 7.176087554009029E-5 0.0 9 0.0 0.0 0.0 2.1528262662027088E-4 0.0 10-11 0.0 0.0 0.0 2.1528262662027088E-4 0.0 12-13 0.0 0.0 0.0 2.1528262662027088E-4 0.0 14-15 0.0 0.0 0.0 2.1528262662027088E-4 0.0 16-17 0.0 0.0 0.0 2.51163064390316E-4 0.0 18-19 0.0 0.0 0.0 2.8704350216036115E-4 0.0 20-21 0.0 0.0 0.0 2.8704350216036115E-4 0.0 22-23 0.0 0.0 0.0 2.8704350216036115E-4 0.0 24-25 0.0 0.0 0.0 2.8704350216036115E-4 0.0 26-27 0.0 0.0 0.0 4.6644569101058687E-4 7.176087554009029E-5 28-29 0.0 0.0 0.0 0.0011122935708713995 7.176087554009029E-5 30-31 0.0 0.0 0.0 0.0021528262662027084 7.176087554009029E-5 32-33 0.0 0.0 0.0 0.0063149570475279455 7.176087554009029E-5 34-35 0.0 0.0 0.0 0.011732903150804762 7.176087554009029E-5 36-37 0.0 0.0 0.0 0.022353512730738125 7.176087554009029E-5 38-39 0.0 0.0 0.0 0.038176785787328034 7.176087554009029E-5 40-41 0.0 0.0 0.0 0.05593760248350038 7.176087554009029E-5 42-43 0.0 0.0 0.0 0.07488247362608422 7.176087554009029E-5 44-45 0.0 0.0 0.0 0.09741538854567257 7.176087554009029E-5 46-47 0.0 0.0 0.0 0.12217289060700373 7.176087554009029E-5 48-49 0.0 0.0 0.0 0.15281478446262226 7.176087554009029E-5 50-51 0.0 0.0 0.0 0.18586066764883385 7.176087554009029E-5 52-53 0.0 0.0 0.0 0.2187271486461952 7.176087554009029E-5 54-55 0.0 0.0 0.0 0.2559710430515021 7.176087554009029E-5 56-57 0.0 0.0 0.0 0.2987764053111659 7.176087554009029E-5 58-59 0.0 0.0 0.0 0.3459591809787753 7.176087554009029E-5 60-61 0.0 7.176087554009029E-5 0.0 0.40379844666408804 7.176087554009029E-5 62-63 0.0 7.176087554009029E-5 0.0 0.4616377123494008 7.176087554009029E-5 64-65 0.0 7.176087554009029E-5 0.0 0.5239979131937393 7.176087554009029E-5 66-67 0.0 7.176087554009029E-5 0.0 0.597624571497872 7.176087554009029E-5 68-69 0.0 7.176087554009029E-5 0.0 0.6780326325405431 7.176087554009029E-5 70-71 0.0 7.176087554009029E-5 0.0 0.7668725964591749 7.176087554009029E-5 72-73 0.0 7.176087554009029E-5 0.0 0.8623863218030351 1.4352175108018058E-4 74-75 0.0 7.176087554009029E-5 0.0 0.9604475582285684 1.4352175108018058E-4 76-77 0.0 7.176087554009029E-5 0.0 1.070421099993757 1.4352175108018058E-4 78-79 0.0 7.176087554009029E-5 0.0 1.1965767191932355 1.4352175108018058E-4 80-81 0.0 7.176087554009029E-5 0.0 1.3362951438697914 1.4352175108018058E-4 82-83 0.0 7.176087554009029E-5 0.0 1.487746471697152 1.4352175108018058E-4 84-85 0.0 7.176087554009029E-5 0.0 1.639771886528833 1.4352175108018058E-4 86-87 0.0 7.176087554009029E-5 0.0 1.7983275410346626 2.1528262662027088E-4 88-89 0.0 7.176087554009029E-5 0.0 1.9761868710607766 2.8704350216036115E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2055 0.0 55.718872 1 GTATCAA 3630 0.0 38.48024 1 TATCACG 120 1.5035403E-7 31.673075 2 TCAACGC 4530 0.0 30.40908 4 ATCAACG 4585 0.0 30.046461 3 CAACGCA 4625 0.0 29.751328 5 TATCAAC 4690 0.0 29.275644 2 AACGCAG 4825 0.0 28.71273 6 ATCACGC 140 5.8027945E-7 27.145424 3 GTGGTAT 1000 0.0 26.606337 1 TGGTATC 1030 0.0 24.907951 2 CGCAGAG 5495 0.0 24.866446 8 ACGCAGA 5555 0.0 24.683271 7 CAGTGCG 215 1.6862032E-9 24.274101 9 GTACATG 3115 0.0 23.79379 1 GTATACG 180 1.9471554E-7 23.75566 1 GGGACGC 120 1.724604E-4 23.722418 6 TACATGG 3195 0.0 22.304981 2 CGTATAC 130 2.9400608E-4 21.92515 3 ACATGGG 3100 0.0 21.3004 3 >>END_MODULE