FastQCFastQC Report
Sat 18 Jun 2016
SRR1931014_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931014_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1851350
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN792904.282820644394631No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21810.11780592540578495No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16150.056.7388951
GTATCAA30200.044.0724071
ATCAACG35850.037.7730143
TCAACGC37900.035.5942084
TATCAAC37550.035.3036352
CAACGCA38950.034.6235735
AACGCAG43250.031.3888266
ACGCAGA48200.027.6650457
CGCAGAG48950.026.430088
GTGGTAT8150.025.7529281
TAGACCG1000.001181769825.1602275
GTACATG31850.024.2698961
ACATGGG30800.023.6993163
ACGGGCG1301.990894E-423.2248275
TACATGG32300.022.818632
TGGTATC8700.022.3559932
GCAGAGT54850.022.2220029
CGCCGTA703.3988363E-521.84411294-95
GAGTACT36950.020.91121312-13
CATGGGG20200.020.4336644
Produced by FastQC (version 0.11.5)