FastQCFastQC Report
Sat 18 Jun 2016
SRR1931014_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931014_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1851350
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56180.30345423609798255No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53260.28768196181165095No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31830.17192859264860777No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28000.061.1169781
GTATCAA50600.040.7714921
ATCAACG65500.031.2030853
CAACGCA67450.030.3132215
TCAACGC67500.030.2081344
TATCAAC68650.029.7737522
GTGGTAT11700.029.6588571
AACGCAG69800.029.494156
TGGTATC12100.026.71342
TAACGCA1309.975927E-625.593234
ACGCAGA80200.025.4328867
CGCAGAG80150.025.2712028
ACATGGG46200.022.530593
GTACATG49650.021.8289591
GCAGAGT91950.021.7701939
TACATGG49300.021.5976642
GAGTACT67000.020.85028812-13
AGAGTAC85100.020.20598410-11
CAGAGTA91250.020.0917710-11
ATAAGCG1507.690454E-419.0121123
Produced by FastQC (version 0.11.5)