##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931014_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1851350 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.259274583412104 31.0 31.0 34.0 30.0 34.0 2 31.42644016528479 31.0 31.0 34.0 30.0 34.0 3 31.482502498177006 31.0 31.0 34.0 30.0 34.0 4 35.265828179436625 37.0 35.0 37.0 33.0 37.0 5 35.14217084829989 37.0 35.0 37.0 32.0 37.0 6 35.17246549815 37.0 35.0 37.0 32.0 37.0 7 35.0520431036811 37.0 35.0 37.0 32.0 37.0 8 35.0548777918816 37.0 35.0 37.0 32.0 37.0 9 36.54240149080401 38.0 35.0 39.0 32.0 39.0 10-11 36.48311610446431 38.0 35.0 39.0 32.0 39.0 12-13 36.366107165041726 38.0 35.0 39.0 32.0 39.0 14-15 37.39782915170011 39.0 36.0 41.0 32.0 41.0 16-17 37.35048505144894 39.0 36.0 41.0 32.0 41.0 18-19 37.38739865503551 39.0 36.0 41.0 32.0 41.0 20-21 37.37440057255516 39.0 36.0 41.0 32.0 41.0 22-23 37.27617495341238 39.0 36.0 40.0 31.0 41.0 24-25 37.141515650741354 39.0 36.0 40.0 31.0 41.0 26-27 36.87388878386042 39.0 36.0 40.0 30.5 41.0 28-29 36.6728641261782 39.0 35.0 40.0 30.0 41.0 30-31 36.43229211116213 38.0 35.0 40.0 30.0 41.0 32-33 36.24997218246145 38.0 35.0 40.0 30.0 41.0 34-35 36.31364571798957 38.0 35.0 40.0 30.0 41.0 36-37 36.36572771221 38.0 35.0 40.0 30.0 41.0 38-39 36.344539120101544 38.0 35.0 40.0 30.0 41.0 40-41 36.216562508439786 38.0 35.0 40.0 30.0 41.0 42-43 36.06554352229455 38.0 35.0 40.0 29.5 41.0 44-45 35.879132524914255 38.0 35.0 40.0 29.0 41.0 46-47 35.61790315175412 38.0 34.5 40.0 27.5 41.0 48-49 35.387580954438654 38.0 34.0 40.0 27.0 41.0 50-51 35.115541631782214 38.0 34.0 40.0 26.5 41.0 52-53 34.90223539579226 38.0 34.0 40.0 26.0 41.0 54-55 34.7440116671618 38.0 33.0 40.0 26.0 41.0 56-57 34.566914413806145 38.0 33.0 40.0 25.0 41.0 58-59 34.35693331892943 38.0 33.0 40.0 25.0 41.0 60-61 34.04399789342912 37.0 33.0 40.0 24.0 41.0 62-63 33.79249844707916 37.0 32.5 40.0 23.0 41.0 64-65 33.47219110378913 36.5 32.0 39.5 23.0 41.0 66-67 33.14613606287304 36.0 32.0 39.0 22.0 40.5 68-69 32.80441623680017 36.0 31.0 39.0 22.0 40.0 70-71 32.42637210684096 35.0 31.0 38.5 21.5 40.0 72-73 32.004380857212304 35.0 31.0 38.0 20.0 40.0 74-75 31.483809652415808 35.0 30.0 37.0 18.5 39.5 76-77 30.132352067410267 33.5 28.5 36.0 18.0 38.5 78-79 30.855906230588488 34.0 30.0 36.0 18.0 39.0 80-81 30.815884894806494 34.0 30.0 36.0 18.0 38.5 82-83 30.532274826477973 34.0 30.0 36.0 18.0 37.0 84-85 30.18300105328544 34.0 30.0 35.0 16.0 37.0 86-87 29.818665568369028 34.0 29.0 35.0 12.0 36.5 88-89 29.448856240041053 34.0 29.0 35.0 7.0 36.0 90-91 29.088494882113054 34.0 29.0 35.0 4.5 36.0 92-93 28.741120803737815 34.0 29.0 35.0 2.0 35.5 94-95 28.335280471007643 34.0 29.0 35.0 2.0 35.0 96-97 27.870713533367542 33.0 28.0 35.0 2.0 35.0 98-99 27.348364706835554 33.0 27.0 35.0 2.0 35.0 100-101 26.186029113889866 32.0 25.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.36278222438916075 1101 2 0.3839351942730467 1101 3 0.30717762870589027 1101 4 0.15018186023358027 1101 5 0.2701654411623835 1101 6 0.3882343980013587 1101 7 0.4392919833199045 1101 8 0.4131451512354971 1101 9 0.4391349189237488 1101 10-11 0.3802654957740117 1101 12-13 0.4162309471838199 1101 14-15 0.575405170921087 1101 16-17 0.6729338515929442 1101 18-19 0.6643244874776073 1101 20-21 0.5868712435105365 1101 22-23 0.6393274384108736 1101 24-25 0.4255975972249715 1101 26-27 0.4660462055342762 1101 28-29 0.5756938454848353 1101 30-31 0.59922769428929 1101 32-33 0.5988847560687205 1101 34-35 0.6585989434779336 1101 36-37 0.6640725493505073 1101 38-39 0.5635818469699956 1101 40-41 0.5289874840302886 1101 42-43 0.6480828064072242 1101 44-45 0.4922039717644182 1101 46-47 0.6343944978801019 1101 48-49 0.5414580276068008 1101 50-51 0.5975686757976391 1101 52-53 0.40200337664651187 1101 54-55 0.3414931731613038 1101 56-57 0.5454788179338266 1101 58-59 0.5102293460664242 1101 60-61 0.23390921472782367 1101 62-63 0.07738127835702358 1101 64-65 0.3667563801520046 1101 66-67 0.420325631445138 1101 68-69 0.34949406530773786 1101 70-71 0.3677200913549541 1101 72-73 0.4195083737299363 1101 74-75 0.36977291396613765 1101 76-77 0.22345177616536205 1101 78-79 0.13113787023296908 1101 80-81 0.21058897946488386 1101 82-83 0.24940289169686025 1101 84-85 0.12644862147903524 1101 86-87 0.20977214626993046 1101 88-89 0.1903596330707984 1101 90-91 0.16796588340342922 1101 92-93 0.14696217045202786 1101 94-95 0.3506817251006993 1101 96-97 0.5095429798604698 1101 98-99 0.5738823952360583 1101 100-101 0.8457665982081899 1102 1 0.23125992813082874 1102 2 0.387146256031631 1102 3 0.34681665107791204 1102 4 0.09051540482675335 1102 5 0.3002281998294123 1102 6 0.36454369295128686 1102 7 0.3866052720031874 1102 8 0.42661632612571054 1102 9 0.49819763674428685 1102 10-11 0.4215419356459904 1102 12-13 0.5287456552170084 1102 14-15 0.6486106591091954 1102 16-17 0.4863541660055688 1102 18-19 0.5396875930763443 1102 20-21 0.5722268518005293 1102 22-23 0.6086679255909928 1102 24-25 0.5656935733179083 1102 26-27 0.5137651921722011 1102 28-29 0.5306539937971451 1102 30-31 0.585102316623896 1102 32-33 0.6604301503625933 1102 34-35 0.6190243531617128 1102 36-37 0.744801171808966 1102 38-39 0.5721377388137441 1102 40-41 0.7645919265480643 1102 42-43 0.9483659648692324 1102 44-45 1.02497735671367 1102 46-47 0.9704143888067733 1102 48-49 0.8164000189921481 1102 50-51 0.6884309190082192 1102 52-53 0.548199843029181 1102 54-55 0.7815438228182643 1102 56-57 0.6551536402845102 1102 58-59 0.7036887677063675 1102 60-61 0.5067967590495712 1102 62-63 0.4168714086573502 1102 64-65 0.5633464881844006 1102 66-67 0.5416484255036949 1102 68-69 0.6804491223017806 1102 70-71 0.6994758714637683 1102 72-73 0.548964627789875 1102 74-75 0.549696992185865 1102 76-77 0.6180782217690286 1102 78-79 0.802666675578326 1102 80-81 0.7358728296820978 1102 82-83 0.6895820101767391 1102 84-85 0.8617858488451375 1102 86-87 0.8126705346779595 1102 88-89 0.7508859731323838 1102 90-91 0.8650091240327846 1102 92-93 0.6436944506484608 1102 94-95 0.8575469672347822 1102 96-97 0.8305059294519381 1102 98-99 0.93752611363767 1102 100-101 1.1980535409005562 1103 1 0.3748729044899157 1103 2 0.3503996157373699 1103 3 0.2809332379344873 1103 4 0.26652357476350375 1103 5 0.2763869093718938 1103 6 0.24643568917617387 1103 7 0.316565623021269 1103 8 0.269004973941783 1103 9 0.35880364257737085 1103 10-11 0.41697526635413595 1103 12-13 0.3732122918446166 1103 14-15 0.4167952883182693 1103 16-17 0.4194763651748872 1103 18-19 0.4053267344066924 1103 20-21 0.40317281118066006 1103 22-23 0.5000605718732771 1103 24-25 0.4990728259649373 1103 26-27 0.6654576007671622 1103 28-29 0.5421161774993664 1103 30-31 0.4516756814422962 1103 32-33 0.6785659122982892 1103 34-35 0.7303536850163326 1103 36-37 0.6444303744267543 1103 38-39 0.6850513515447929 1103 40-41 0.67840567832814 1103 42-43 0.6122387834328649 1103 44-45 0.5099734899373516 1103 46-47 0.6401873012317125 1103 48-49 0.5385590358110122 1103 50-51 0.3591882778257016 1103 52-53 0.5111698112815901 1103 54-55 0.41500725370109137 1103 56-57 0.5427099796673929 1103 58-59 0.567540283495596 1103 60-61 0.5144418729032125 1103 62-63 0.3477263470923333 1103 64-65 0.27682288148267986 1103 66-67 0.29967330972634443 1103 68-69 0.08885029098489383 1103 70-71 0.3107277386842213 1103 72-73 0.29245262787250326 1103 74-75 0.3661855977837192 1103 76-77 0.5116673777531062 1103 78-79 0.5719529809929718 1103 80-81 0.5530870206866432 1103 82-83 0.5343381063206785 1103 84-85 0.4909924689727738 1103 86-87 0.4648678835799629 1103 88-89 0.40971106702597737 1103 90-91 0.3475842292233011 1103 92-93 0.31077301398935475 1103 94-95 0.5152294577744172 1103 96-97 0.7413606613145625 1103 98-99 0.73717003453595 1103 100-101 0.8337995963368279 1104 1 0.34765031796805346 1104 2 0.4135474404505928 1104 3 0.4357230816227755 1104 4 0.09906253984883051 1104 5 0.2985175481974309 1104 6 0.3656596348212773 1104 7 0.3224284092489853 1104 8 0.3180783502973057 1104 9 0.5379242998714346 1104 10-11 0.4103751181545334 1104 12-13 0.4285120575086978 1104 14-15 0.512665008080603 1104 16-17 0.6009174370266166 1104 18-19 0.5566704985511635 1104 20-21 0.5075152350089454 1104 22-23 0.5969125214753888 1104 24-25 0.49162911861778014 1104 26-27 0.5303720349318084 1104 28-29 0.5885645903719166 1104 30-31 0.6544167471503854 1104 32-33 0.6100681070817089 1104 34-35 0.7163854338343114 1104 36-37 0.6907109029802498 1104 38-39 0.762131944794568 1104 40-41 0.6455888703368942 1104 42-43 0.5942049482854799 1104 44-45 0.8874433892443747 1104 46-47 0.5830246339899716 1104 48-49 0.5717680336080733 1104 50-51 0.3618314214627105 1104 52-53 0.448848870126362 1104 54-55 0.5782182701221501 1104 56-57 0.7783838302114816 1104 58-59 0.733484466661281 1104 60-61 0.6368319237198818 1104 62-63 0.6045171361486368 1104 64-65 0.6336410034602267 1104 66-67 0.7492495867567825 1104 68-69 0.715046764536531 1104 70-71 0.8039531863861882 1104 72-73 0.5599120328423304 1104 74-75 0.5748533937096099 1104 76-77 0.588943439003927 1104 78-79 0.5901367710661098 1104 80-81 0.547410173551647 1104 82-83 0.6651591095391929 1104 84-85 0.7629300847145295 1104 86-87 0.6387836254618975 1104 88-89 0.8545010737087502 1104 90-91 0.7096621832162278 1104 92-93 0.7329070380849565 1104 94-95 1.0045909331503395 1104 96-97 1.097374217440457 1104 98-99 1.0835894845424114 1104 100-101 1.2947426071960244 1105 1 0.23237345071019533 1105 2 0.4237450705567909 1105 3 0.32292037476078406 1105 4 0.3882207000124396 1105 5 0.18797858271458523 1105 6 0.2801873309762186 1105 7 0.2067979487142111 1105 8 0.3415447852383764 1105 9 0.2872666163763782 1105 10-11 0.3034833742721972 1105 12-13 0.2456998669028181 1105 14-15 0.41453431305765776 1105 16-17 0.4654699963719793 1105 18-19 0.5246467341053318 1105 20-21 0.481367446418524 1105 22-23 0.38007857830737635 1105 24-25 0.35542995106320063 1105 26-27 0.4496856471067332 1105 28-29 0.5468023708086065 1105 30-31 0.32051588626862326 1105 32-33 0.4881817750375248 1105 34-35 0.5955122395793424 1105 36-37 0.6987264283305308 1105 38-39 0.5994945458912255 1105 40-41 0.5770533122525592 1105 42-43 0.6507414115686032 1105 44-45 0.7365038484838777 1105 46-47 0.7954167011930409 1105 48-49 0.7616409236487485 1105 50-51 0.746131855154168 1105 52-53 0.6091309656020059 1105 54-55 0.6641807659100465 1105 56-57 0.7788628560508428 1105 58-59 0.8033748453013629 1105 60-61 0.9349410805698852 1105 62-63 0.8712961917146416 1105 64-65 0.7813364189559024 1105 66-67 0.8577423017427321 1105 68-69 0.6726738831806003 1105 70-71 0.6956186578662198 1105 72-73 0.9056930074893224 1105 74-75 0.863536994475254 1105 76-77 0.837516907250258 1105 78-79 1.0394391873612747 1105 80-81 0.8610173857627004 1105 82-83 0.8436748316610334 1105 84-85 0.8969470234453745 1105 86-87 1.0181005031017492 1105 88-89 1.0708265869649018 1105 90-91 0.9219799337972354 1105 92-93 0.9145847525771167 1105 94-95 0.9338106160627717 1105 96-97 0.937311473864316 1105 98-99 0.9915130508271268 1105 100-101 0.9679435121963031 1106 1 0.17305141681189085 1106 2 0.1530571343653655 1106 3 0.11404699490036663 1106 4 0.03677503700147611 1106 5 0.08977319886613344 1106 6 0.19643857723742997 1106 7 0.16342805838520036 1106 8 0.21093660976479356 1106 9 0.20501337609721304 1106 10-11 0.08962494954637634 1106 12-13 0.23897005633451585 1106 14-15 0.16129403389514607 1106 16-17 0.17110309706988858 1106 18-19 0.11562380289895202 1106 20-21 0.0648669479140338 1106 22-23 0.19239163912492074 1106 24-25 0.2342933608338882 1106 26-27 0.1171831545843034 1106 28-29 0.19834773471687583 1106 30-31 0.22854180850192307 1106 32-33 0.39969722867660806 1106 34-35 0.252790405082834 1106 36-37 0.2428440753893497 1106 38-39 0.11943472535283917 1106 40-41 0.17052290347888288 1106 42-43 0.25941538963440536 1106 44-45 0.4157162113951429 1106 46-47 0.13265498633760586 1106 48-49 0.11956714498474952 1106 50-51 0.07364930281119797 1106 52-53 0.11386675822414105 1106 54-55 0.18237617966877906 1106 56-57 0.35039824986938584 1106 58-59 0.30163007959847477 1106 60-61 0.2911225362029839 1106 62-63 0.04676977097685153 1106 64-65 0.07196453460894503 1106 66-67 0.13466154401591268 1106 68-69 0.05527607660034306 1106 70-71 0.21481108059802523 1106 72-73 0.09911274826698602 1106 74-75 0.3737563364493326 1106 76-77 0.18048799399004167 1106 78-79 0.12318794110005982 1106 80-81 0.17881399593219172 1106 82-83 0.20309656645664376 1106 84-85 0.2912640722988158 1106 86-87 0.4034444711974636 1106 88-89 0.17426626792203237 1106 90-91 0.2175930943156814 1106 92-93 0.23736640761201144 1106 94-95 0.4101316529521064 1106 96-97 0.350073188719751 1106 98-99 0.48428473577229525 1106 100-101 0.6277142001324982 1107 1 0.20066101226794686 1107 2 0.22149978620781852 1107 3 0.17626438313968862 1107 4 -0.004895909754218053 1107 5 0.17537044752420172 1107 6 0.16106943214744973 1107 7 0.17248120198470218 1107 8 0.17129996414609394 1107 9 0.16809937063884206 1107 10-11 0.12875553758024694 1107 12-13 0.16817260021802127 1107 14-15 0.19605641342322144 1107 16-17 0.1993990173897302 1107 18-19 0.23479331456596952 1107 20-21 0.22828603408985515 1107 22-23 0.3319970652707269 1107 24-25 0.2826644093046369 1107 26-27 0.30615487578433687 1107 28-29 0.38907378887763144 1107 30-31 0.3056206729187707 1107 32-33 0.3704863763849957 1107 34-35 0.2809178913110202 1107 36-37 0.44943062886025587 1107 38-39 0.431531264019668 1107 40-41 0.41394113363689655 1107 42-43 0.4621261966074286 1107 44-45 0.39901631109604097 1107 46-47 0.3072158995575407 1107 48-49 0.28156020620512123 1107 50-51 0.3527899112887951 1107 52-53 0.4028472913721757 1107 54-55 0.38356881388732944 1107 56-57 0.35908531997220905 1107 58-59 0.3352189594236421 1107 60-61 0.3557211645603431 1107 62-63 0.2546189507103733 1107 64-65 0.09054893292913135 1107 66-67 0.1719693176646615 1107 68-69 0.2504002998900674 1107 70-71 0.2757119638069838 1107 72-73 0.2911346623228006 1107 74-75 0.25892406558515546 1107 76-77 0.35291868172481244 1107 78-79 0.4139109740214941 1107 80-81 0.3504886185290452 1107 82-83 0.3567371598280431 1107 84-85 0.36415927557786887 1107 86-87 0.2635950799771827 1107 88-89 0.34575030067226464 1107 90-91 0.4772333654215579 1107 92-93 0.3164793213959136 1107 94-95 0.35020134287224636 1107 96-97 0.5797833008077298 1107 98-99 0.7512739197355565 1107 100-101 1.0525354434731788 1108 1 0.052847127035189345 1108 2 0.09947499511163471 1108 3 -0.04234205064398111 1108 4 0.025723817220978162 1108 5 -0.00826344507125043 1108 6 0.015227840998818465 1108 7 0.09331737197214807 1108 8 0.18335410515680195 1108 9 0.13421055739406285 1108 10-11 0.06746464563534005 1108 12-13 0.12274944706896207 1108 14-15 -0.025627727032755843 1108 16-17 0.04329630277478458 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-1.3980045779801493 2316 98-99 -1.5454230499614177 2316 100-101 -1.9823231423854075 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 14.0 4 25.0 5 37.0 6 113.0 7 292.0 8 704.0 9 1328.0 10 2473.0 11 3811.0 12 5491.0 13 6921.0 14 8621.0 15 10942.0 16 12997.0 17 15273.0 18 13281.0 19 10344.0 20 10004.0 21 10586.0 22 11436.0 23 13448.0 24 16682.0 25 21057.0 26 26614.0 27 34570.0 28 43281.0 29 53618.0 30 65012.0 31 78331.0 32 93837.0 33 113375.0 34 138813.0 35 172148.0 36 222940.0 37 300465.0 38 281818.0 39 50628.0 40 17.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.42869520453785 22.355722095792565 15.532247844859018 22.68333485481056 2 15.15356617730468 22.18447603624354 40.732714149436006 21.929243637015773 3 18.443352606151745 26.558732401669733 31.35241385568721 23.64550113649131 4 12.474693317088002 18.697459214445935 39.47030989122049 29.357537577245573 5 11.932416964791635 38.8021777211927 36.502703785948526 12.762701528067142 6 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30.939120575620088 20.345834432791612 28-29 20.308586043408923 28.502833746770932 30.927094994321703 20.26148521549844 30-31 20.63310244017854 28.19061730742533 30.91960585928068 20.256674393115446 32-33 20.467223322266854 28.35842425584907 30.700591863031384 20.47376055885269 34-35 20.581015370968515 28.41325124102757 30.85300690891352 20.152726479090393 36-37 20.577841383409893 28.34665297567139 30.830126419344705 20.245379221574012 38-39 20.492879368005593 28.4319701535991 30.932826962876458 20.142323515518846 40-41 20.582913183119956 28.348499072554638 30.829753008930915 20.23883473539449 42-43 20.464659615156066 28.456869820759103 30.53839796017589 20.540072603908943 44-45 20.492893837003905 28.65998455064525 30.455404843318696 20.391716769032147 46-47 20.618126766631146 28.662066841962393 30.153674120111333 20.566132271295125 48-49 20.692355250857638 28.77736899494614 29.88227108469043 20.648004669505788 50-51 20.810105745723256 28.93986963330444 29.709072097782595 20.54095252318971 52-53 20.76294042180996 29.013960744446866 29.614271403793612 20.60882742994956 54-55 20.661317535171207 28.966697766687176 29.716069598496393 20.655915099645224 56-57 20.69767875263103 29.112730519718166 29.532992892002024 20.656597835648782 58-59 20.702718866262508 29.0126663246913 29.71841596792671 20.566198841119483 60-61 20.70500245384009 29.134017524338983 29.722209077991558 20.438770943829372 62-63 20.711654528562985 29.013803438515794 29.66799958420769 20.606542448713533 64-65 20.471846649297547 28.979439683457535 29.723691306127485 20.82502236111743 66-67 20.494523137436246 29.026803027341263 29.628015198435754 20.850658636786733 68-69 20.57095162394199 29.03909004669537 29.61739467551482 20.77256365384782 70-71 20.64609421884783 28.84523401229323 29.61080647680477 20.897865292054174 72-73 20.675122262615773 29.071453302172596 29.505620834415303 20.74780360079633 74-75 20.70084026969641 29.17106422428708 29.334358241785612 20.793737264230895 76-77 20.524943545364632 29.19088228887184 29.38228007723021 20.901894088533314 78-79 20.4722620904162 29.083413864984475 29.510074063910714 20.93424998068861 80-81 20.58961346283533 29.124419308412218 29.33934372701844 20.946623501734006 82-83 20.67502093067221 29.176195749047988 29.428687174224216 20.72009614605558 84-85 20.724579361006835 29.168228589947876 29.236665136251926 20.870526912793366 86-87 20.515920814540742 29.234153455586465 29.358036027763525 20.89188970210927 88-89 20.6488628681881 29.075540424226364 29.26904823057164 21.006548477013894 90-91 20.637625403245845 29.04698934977215 29.432787317391483 20.88259792959052 92-93 20.652311030206047 29.0917872386519 29.30415852806329 20.951743203078763 94-95 20.33047730990033 29.06932023689402 29.393985389663 21.206217063542653 96-97 20.381586409917087 28.950306533070464 29.40756745077916 21.26053960623329 98-99 20.25467901801388 29.025629945715288 29.304912631323088 21.41477840494774 100-101 20.38693385907581 28.979933561995303 29.24549652955951 21.38763604936938 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 304.0 1 220.5 2 194.5 3 267.5 4 221.5 5 229.0 6 411.0 7 871.5 8 1454.5 9 1787.0 10 1631.5 11 1291.5 12 1292.5 13 1456.5 14 1647.0 15 1899.0 16 2156.0 17 2476.5 18 2860.0 19 3172.5 20 3694.0 21 4435.0 22 5421.0 23 6930.5 24 8950.5 25 11577.5 26 14668.5 27 18081.5 28 21920.5 29 26895.5 30 32423.0 31 38015.5 32 44251.5 33 49576.5 34 53601.5 35 57777.5 36 63144.0 37 68949.5 38 72460.0 39 73184.5 40 72979.0 41 72745.5 42 73437.0 43 74815.0 44 75163.5 45 76523.5 46 76975.0 47 75105.5 48 72989.5 49 69845.0 50 65862.5 51 60351.5 52 53689.0 53 47522.0 54 41652.5 55 34846.0 56 27850.5 57 21787.5 58 16687.0 59 12462.5 60 9052.0 61 6467.0 62 4495.0 63 2865.0 64 1609.0 65 877.5 66 455.0 67 217.0 68 127.0 69 81.5 70 48.0 71 34.0 72 22.5 73 18.5 74 12.5 75 6.0 76 6.5 77 5.5 78 5.0 79 2.5 80 1.0 81 0.5 82 0.5 83 0.5 84 0.0 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0042671563993842325 2 0.005185405244821346 3 0.002052556242741783 4 0.16334026521187242 5 0.003078834364112675 6 6.481756556026683E-4 7 0.0 8 7.562049315364464E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 5.401463796688902E-4 24-25 5.671536986523348E-4 26-27 0.003213870959029897 28-29 1.3503659491722255E-4 30-31 0.007183946849596241 32-33 0.022416074756258945 34-35 0.014205849785291816 36-37 6.211683366192238E-4 38-39 2.4306587085100062E-4 40-41 0.0015124098630728928 42-43 0.011343073973046696 44-45 0.007562049315364464 46-47 0.010397817808626138 48-49 0.01028978853269236 50-51 0.17997677370567422 52-53 0.11113511761687417 54-55 0.017986874442974046 56-57 0.006778837064844573 58-59 0.018337969589758828 60-61 0.11923731331190753 62-63 0.2308585626704837 64-65 0.23734031922651036 66-67 0.24744105652631862 68-69 0.24230966591946415 70-71 0.22877899910875846 72-73 0.1550220109649715 74-75 0.2528425203230075 76-77 0.19593809922488994 78-79 0.005995624814324682 80-81 8.102195695033353E-5 82-83 0.0 84-85 0.0 86-87 0.0 88-89 1.890512328841116E-4 90-91 0.006589785831960461 92-93 0.01871607205552705 94-95 0.0031328490020795636 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1851350.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.20084584942642 #Duplication Level Percentage of deduplicated Percentage of total 1 76.08949442304583 46.567414189456194 2 14.531662019664482 17.787000144029015 3 4.440721592291415 8.153277528901391 4 1.8204278779058538 4.456469037428584 5 0.9380840777799745 2.8705769519006785 6 0.535275898102147 1.9655602635997673 7 0.3263979465819612 1.3983081290032349 8 0.22989427970577397 1.1255779499150396 9 0.17065458941749106 0.9399784698393325 >10 0.847515024637577 9.216282295835995 >50 0.047034743830589844 1.9710490641299543 >100 0.02177570231930095 2.3852280037364637 >500 6.193977519355978E-4 0.2697401228829724 >1k 2.6545617940097E-4 0.3008785630872903 >5k 1.7697078626731334E-4 0.5926592862540145 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5618 0.30345423609798255 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5326 0.28768196181165095 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3183 0.17192859264860777 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 5.401463796688903E-5 0.0 8 0.0 0.0 0.0 5.401463796688903E-5 0.0 9 0.0 0.0 0.0 5.401463796688903E-5 0.0 10-11 0.0 0.0 0.0 1.0802927593377806E-4 0.0 12-13 0.0 0.0 0.0 1.0802927593377806E-4 0.0 14-15 0.0 2.7007318983444514E-5 0.0 1.0802927593377806E-4 0.0 16-17 0.0 5.401463796688903E-5 0.0 1.620439139006671E-4 0.0 18-19 0.0 5.401463796688903E-5 0.0 1.620439139006671E-4 0.0 20-21 0.0 5.401463796688903E-5 0.0 1.620439139006671E-4 0.0 22-23 0.0 1.0802927593377806E-4 0.0 1.620439139006671E-4 0.0 24-25 0.0 1.0802927593377806E-4 0.0 2.160585518675561E-4 0.0 26-27 0.0 3.240878278013342E-4 0.0 3.510951467847787E-4 0.0 28-29 0.0 3.510951467847787E-4 0.0 7.291976125530018E-4 0.0 30-31 0.0 4.8613174170200125E-4 0.0 0.002322629432576228 0.0 32-33 0.0 6.211683366192239E-4 0.0 0.006751829745861129 0.0 34-35 0.0 8.102195695033354E-4 0.0 0.01245037405136792 0.0 36-37 0.0 8.102195695033354E-4 0.0 0.023712426067464282 0.0 38-39 0.0 8.102195695033354E-4 0.0 0.04024090528533233 0.0 40-41 0.0 9.182488454371134E-4 0.0 0.05949712372052827 0.0 42-43 0.0 9.182488454371134E-4 0.0 0.07907742998352554 0.0 44-45 0.0 9.182488454371134E-4 0.0 0.10381613417236071 0.0 46-47 0.0 9.182488454371134E-4 0.0 0.13465849245145434 0.0 48-49 0.0 9.182488454371134E-4 0.0 0.1685526777756772 0.0 50-51 0.0 0.0011883220352715586 0.0 0.20603883652469818 0.0 52-53 0.0 0.0014583952251060036 0.0 0.24414616361033842 0.0 54-55 0.0 0.0014583952251060036 0.0 0.2868717422421476 0.0 56-57 0.0 0.0014583952251060036 0.0 0.3369163043184703 0.0 58-59 0.0 0.0015124098630728928 0.0 0.3955221865125449 0.0 60-61 0.0 0.0016474464579901153 0.0 0.45804412995921895 0.0 62-63 0.0 0.001728468414940449 0.0 0.5261835957544494 0.0 64-65 0.0 0.0017824830529073379 0.0 0.5971048154049747 0.0 66-67 0.0 0.0018094903718907825 0.0 0.6809895481675534 0.0 68-69 0.0 0.0018364976908742268 0.0 0.7803224673886624 0.0 70-71 0.0 0.001890512328841116 0.0 0.8899181678234802 0.0 72-73 0.0 0.001890512328841116 0.0 1.008750371350636 2.7007318983444514E-5 74-75 0.0 0.001890512328841116 0.0 1.1323088556998946 5.401463796688903E-5 76-77 0.0 0.001890512328841116 0.0 1.2702082264293624 5.401463796688903E-5 78-79 0.0 0.001890512328841116 0.0 1.429848488940503 5.401463796688903E-5 80-81 0.0 0.001890512328841116 0.0 1.607799713722419 5.401463796688903E-5 82-83 0.0 0.001890512328841116 0.0 1.7961757636319442 5.401463796688903E-5 84-85 0.0 0.001890512328841116 0.0 1.9895211602344236 5.401463796688903E-5 86-87 0.0 0.001890512328841116 0.0 2.197396494449996 5.401463796688903E-5 88-89 0.0 0.001890512328841116 0.0 2.436303238177546 5.401463796688903E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2800 0.0 61.116978 1 GTATCAA 5060 0.0 40.771492 1 ATCAACG 6550 0.0 31.203085 3 CAACGCA 6745 0.0 30.313221 5 TCAACGC 6750 0.0 30.208134 4 TATCAAC 6865 0.0 29.773752 2 GTGGTAT 1170 0.0 29.658857 1 AACGCAG 6980 0.0 29.49415 6 TGGTATC 1210 0.0 26.7134 2 TAACGCA 130 9.975927E-6 25.59323 4 ACGCAGA 8020 0.0 25.432886 7 CGCAGAG 8015 0.0 25.271202 8 ACATGGG 4620 0.0 22.53059 3 GTACATG 4965 0.0 21.828959 1 GCAGAGT 9195 0.0 21.770193 9 TACATGG 4930 0.0 21.597664 2 GAGTACT 6700 0.0 20.850288 12-13 AGAGTAC 8510 0.0 20.205984 10-11 CAGAGTA 9125 0.0 20.09177 10-11 ATAAGCG 150 7.690454E-4 19.012112 3 >>END_MODULE