FastQCFastQC Report
Sat 18 Jun 2016
SRR1931013_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931013_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1704270
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN701244.114606253703932No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21020.12333726463529839No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15950.058.856561
GTATCAA32600.046.9506261
ATCAACG38550.039.188663
CAACGCA39950.037.5544365
TCAACGC40600.037.452364
TATCAAC40950.036.6263282
AACGCAG44250.034.166766
TATACCG1950.033.4514965
GTGGTAT7450.030.8172571
ACGCAGA49050.030.2723647
CGCAGAG49150.029.6652858
TGGTATC8000.028.0583842
GCAGAGT54600.025.8622579
GTACATG37700.025.4421751
TACATGG37400.024.9620042
ACATGGG36500.024.7512973
GTATAGG4900.023.9480131
ATACCGA1703.5774356E-622.0270146
GAGTACT36800.021.77135312-13
AGAGTAC50400.021.76940510-11
Produced by FastQC (version 0.11.5)