##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1931013_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1704270
Sequences flagged as poor quality	0
Sequence length	101
%GC	42
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	23.565498424545407	31.0	10.0	31.0	2.0	34.0
2	22.108619526248773	31.0	2.0	31.0	2.0	34.0
3	22.952945836047107	31.0	10.0	31.0	2.0	34.0
4	24.52280448520481	35.0	2.0	37.0	2.0	37.0
5	23.833839121735405	35.0	2.0	37.0	2.0	37.0
6	24.979854131094253	35.0	10.0	37.0	2.0	37.0
7	25.37283411665992	35.0	10.0	37.0	2.0	37.0
8	25.935149946898086	35.0	17.0	37.0	2.0	37.0
9	27.670247672023798	35.0	17.0	39.0	2.0	39.0
10-11	28.23804942878769	36.0	23.5	39.0	2.0	39.0
12-13	28.24484794076056	37.0	23.0	39.0	2.0	39.0
14-15	28.355859986973897	37.0	14.0	40.0	2.0	41.0
16-17	27.06952595539439	36.0	6.0	39.5	2.0	41.0
18-19	25.849823384792316	35.5	2.0	39.0	2.0	41.0
20-21	25.474037857851165	34.5	2.0	39.0	2.0	41.0
22-23	25.115947003702466	34.0	2.0	39.0	2.0	40.5
24-25	25.01454933783966	34.0	2.0	39.0	2.0	41.0
26-27	24.75793477559307	34.0	2.0	39.0	2.0	40.0
28-29	24.648834398305432	34.0	2.0	39.0	2.0	40.0
30-31	24.817147811086272	34.0	2.0	39.0	2.0	40.0
32-33	24.763228831112443	34.0	2.0	39.0	2.0	40.0
34-35	24.488250101216355	33.0	2.0	38.0	2.0	40.0
36-37	23.944201329601533	33.0	2.0	38.0	2.0	40.0
38-39	23.95857962646764	33.0	2.0	38.0	2.0	40.0
40-41	23.955636430847225	33.0	2.0	38.0	2.0	40.0
42-43	23.8766882594894	32.5	2.0	38.0	2.0	40.0
44-45	23.51526577361568	32.0	2.0	38.0	2.0	40.0
46-47	23.195693464063794	31.0	2.0	38.0	2.0	40.0
48-49	23.09176714957137	31.0	2.0	38.0	2.0	40.0
50-51	20.148541604323256	23.5	2.0	36.5	2.0	39.5
52-53	22.536753272662196	30.0	2.0	37.5	2.0	39.5
54-55	23.532779137108555	32.5	2.0	38.0	2.0	40.0
56-57	23.589521613359388	32.5	2.0	38.0	2.0	40.0
58-59	23.34053964453989	32.0	2.0	38.0	2.0	40.0
60-61	23.0976347644446	31.5	2.0	38.0	2.0	40.0
62-63	22.68646341248746	31.0	2.0	38.0	2.0	40.0
64-65	22.414681945935797	31.0	2.0	37.5	2.0	40.0
66-67	22.13681048190721	30.0	2.0	37.0	2.0	40.0
68-69	21.735029954173925	29.5	2.0	36.0	2.0	39.0
70-71	21.45034530913529	29.0	2.0	36.0	2.0	39.0
72-73	21.080121987713213	29.0	2.0	35.5	2.0	39.0
74-75	20.671073245436464	28.0	2.0	35.0	2.0	38.0
76-77	20.320257060207595	27.5	2.0	35.0	2.0	37.5
78-79	20.009731439267252	26.5	2.0	34.0	2.0	37.0
80-81	19.638318752310372	26.0	2.0	34.0	2.0	36.0
82-83	19.228408057408743	26.0	2.0	34.0	2.0	36.0
84-85	18.90128764808393	25.5	2.0	34.0	2.0	35.5
86-87	18.45082293298597	25.0	2.0	34.0	2.0	35.0
88-89	18.104236711319217	24.0	2.0	34.0	2.0	35.0
90-91	17.678900056915865	21.0	2.0	33.0	2.0	35.0
92-93	17.168335416336614	19.0	2.0	33.0	2.0	35.0
94-95	16.645709893385437	14.0	2.0	32.5	2.0	35.0
96-97	16.030743074747544	4.5	2.0	32.0	2.0	34.5
98-99	15.333297247501864	2.0	2.0	32.0	2.0	34.0
100-101	14.080161594113608	2.0	2.0	30.5	2.0	34.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	8.009877692088917
1101	2	9.924454803286611
1101	3	9.188292800797988
1101	4	11.22083255620312
1101	5	12.15688083669573
1101	6	10.988587736413006
1101	7	10.547897818658075
1101	8	9.88574140465499
1101	9	9.812350109881983
1101	10-11	9.048234702790413
1101	12-13	8.974834676180006
1101	14-15	10.027192527349168
1101	16-17	11.130489264352004
1101	18-19	12.563994005487896
1101	20-21	12.65995667697101
1101	22-23	12.878311054226927
1101	24-25	12.798287056844487
1101	26-27	12.984546457486491
1101	28-29	12.849381688699463
1101	30-31	12.248238083275734
1101	32-33	12.354162551575385
1101	34-35	12.383021130165805
1101	36-37	12.894280801615768
1101	38-39	12.390267253254546
1101	40-41	12.025687762367955
1101	42-43	11.852851723416016
1101	44-45	11.997528238190625
1101	46-47	11.998623371272227
1101	48-49	12.153978501035699
1101	50-51	13.886831336041613
1101	52-53	12.460761431647995
1101	54-55	11.995308083779687
1101	56-57	11.8704133357096
1101	58-59	12.057961696941948
1101	60-61	11.990696947488317
1101	62-63	11.93591094269522
1101	64-65	11.693205659580457
1101	66-67	11.458862953890737
1101	68-69	11.565472174943384
1101	70-71	11.450828969590255
1101	72-73	11.437625179123916
1101	74-75	11.377797149612142
1101	76-77	11.312808710341873
1101	78-79	10.884186624368215
1101	80-81	10.69467166802858
1101	82-83	10.662152181624517
1101	84-85	10.335888553513836
1101	86-87	10.133279040756655
1101	88-89	9.896712419895884
1101	90-91	9.693509775367104
1101	92-93	9.716294518641629
1101	94-95	9.67135799747979
1101	96-97	9.58043261206542
1101	98-99	9.544973929370807
1101	100-101	9.155392982884047
1102	1	8.061339431161102
1102	2	9.6045700238064
1102	3	9.054829994526923
1102	4	11.288816820794523
1102	5	11.968792231469227
1102	6	11.021000437465656
1102	7	10.59249783852368
1102	8	9.741869366882622
1102	9	9.436040654546105
1102	10-11	8.558229874985958
1102	12-13	8.638473570266289
1102	14-15	9.486502971345484
1102	16-17	10.622179203808098
1102	18-19	11.93194460249558
1102	20-21	12.405534846979027
1102	22-23	12.523405346560878
1102	24-25	12.213184876016648
1102	26-27	12.412864822316056
1102	28-29	12.260317936271125
1102	30-31	12.024987642651954
1102	32-33	11.966782929068117
1102	34-35	11.698289754749727
1102	36-37	12.084622478503984
1102	38-39	12.12400748503536
1102	40-41	11.68508586912942
1102	42-43	11.273444446136033
1102	44-45	11.56052300152426
1102	46-47	11.441716203715078
1102	48-49	11.078867161239106
1102	50-51	13.27046479018556
1102	52-53	11.967340181454539
1102	54-55	11.461200453769681
1102	56-57	11.322867546640765
1102	58-59	11.475879492598775
1102	60-61	11.520226536847744
1102	62-63	11.663586100753843
1102	64-65	11.366150493252206
1102	66-67	11.309787166600564
1102	68-69	11.156187544686222
1102	70-71	10.901342818921176
1102	72-73	10.893979747867004
1102	74-75	10.692341892271688
1102	76-77	10.455262147438358
1102	78-79	10.201696482098132
1102	80-81	9.735325708485117
1102	82-83	9.736772944879615
1102	84-85	9.571882723189216
1102	86-87	9.413841757170498
1102	88-89	9.383701602379961
1102	90-91	9.10800421877207
1102	92-93	8.882460791461092
1102	94-95	8.707104540055711
1102	96-97	8.706633788294143
1102	98-99	8.636450429050877
1102	100-101	7.9281535399526195
1103	1	7.921272435192215
1103	2	9.743276970638025
1103	3	8.976009082287515
1103	4	11.11326983329046
1103	5	11.839280455559905
1103	6	10.893341631728159
1103	7	10.34705671290882
1103	8	9.820145743319447
1103	9	9.551425468449061
1103	10-11	8.74433466328799
1103	12-13	8.58326770476405
1103	14-15	9.544421192907034
1103	16-17	10.704966710969646
1103	18-19	12.072316125595773
1103	20-21	12.462059469804107
1103	22-23	12.531095946995478
1103	24-25	12.489394855215892
1103	26-27	12.39419447035495
1103	28-29	12.314317306570953
1103	30-31	11.941174291976058
1103	32-33	12.08263614699083
1103	34-35	11.92359208825339
1103	36-37	12.045202989799872
1103	38-39	11.99789970307678
1103	40-41	11.602477723286736
1103	42-43	11.203705152329292
1103	44-45	11.076597130654093
1103	46-47	11.255252692775326
1103	48-49	11.157712590493329
1103	50-51	13.213506603309131
1103	52-53	11.569136117307707
1103	54-55	11.063245430878535
1103	56-57	11.068520292804507
1103	58-59	10.935737397142422
1103	60-61	10.902361633341556
1103	62-63	10.967033193035892
1103	64-65	10.681516725838677
1103	66-67	10.55510828787979
1103	68-69	10.547228769401435
1103	70-71	10.313524162247127
1103	72-73	10.38022570255956
1103	74-75	10.23744029582691
1103	76-77	9.87615219249805
1103	78-79	9.82325313188677
1103	80-81	9.436095542750099
1103	82-83	9.468220016515751
1103	84-85	9.26087406739262
1103	86-87	9.221335447265108
1103	88-89	9.09013486706565
1103	90-91	9.00166102185657
1103	92-93	8.80511735195618
1103	94-95	8.563339345958378
1103	96-97	8.602734550644266
1103	98-99	8.287748798540358
1103	100-101	7.9560280954204305
1104	1	8.12979539880767
1104	2	9.93121364858241
1104	3	8.928257007476422
1104	4	11.124826714589688
1104	5	12.018488579521385
1104	6	10.999492604125553
1104	7	10.48624617413638
1104	8	9.870817067884186
1104	9	9.53776180027647
1104	10-11	8.701015404859174
1104	12-13	8.607492834279864
1104	14-15	9.35191948567839
1104	16-17	10.827325094407637
1104	18-19	12.076698058779176
1104	20-21	12.27370126765489
1104	22-23	12.446929853112358
1104	24-25	12.301398813539492
1104	26-27	12.59301556502773
1104	28-29	12.256825826031143
1104	30-31	11.955458568344678
1104	32-33	11.968436314488315
1104	34-35	11.982828612269142
1104	36-37	12.273602654804915
1104	38-39	11.987712219342722
1104	40-41	11.66140727980768
1104	42-43	11.240244433046612
1104	44-45	11.399244436124562
1104	46-47	11.469381705874834
1104	48-49	11.367490255938794
1104	50-51	13.46960619201717
1104	52-53	11.96746092005884
1104	54-55	11.437043141523855
1104	56-57	11.013442149557449
1104	58-59	11.382077727332675
1104	60-61	11.286762929945567
1104	62-63	11.369681538414358
1104	64-65	11.350776350270614
1104	66-67	11.102616929682092
1104	68-69	11.107417893632071
1104	70-71	10.733923792773933
1104	72-73	10.449408640727853
1104	74-75	10.207657358548378
1104	76-77	10.060276523231057
1104	78-79	9.524048244430393
1104	80-81	9.34733925603356
1104	82-83	9.4761366637189
1104	84-85	9.144747624589787
1104	86-87	9.141603003915069
1104	88-89	8.999199361381194
1104	90-91	8.825241742073494
1104	92-93	8.348871481002647
1104	94-95	8.321983372660101
1104	96-97	8.226988680802934
1104	98-99	7.796644321708618
1104	100-101	7.028311572116481
1105	1	7.990166511283533
1105	2	9.356560924498872
1105	3	8.656291032563349
1105	4	10.92112243814745
1105	5	11.300591167301338
1105	6	10.698558250761263
1105	7	10.149500648737966
1105	8	9.482483285360207
1105	9	9.19587782842968
1105	10-11	8.509812343440817
1105	12-13	8.204467756186968
1105	14-15	9.174929718388608
1105	16-17	10.32330032780268
1105	18-19	11.120122736135105
1105	20-21	11.768990840982685
1105	22-23	12.041075978511657
1105	24-25	12.293507509575868
1105	26-27	12.195262309868294
1105	28-29	12.13031505429215
1105	30-31	11.516361640366878
1105	32-33	11.21742425482401
1105	34-35	11.474429759693518
1105	36-37	11.672670068748086
1105	38-39	11.509809637748344
1105	40-41	11.312849237012326
1105	42-43	11.025454996685149
1105	44-45	11.045816080559973
1105	46-47	10.934969468154303
1105	48-49	10.897139876188607
1105	50-51	12.896837859515944
1105	52-53	11.21694177349162
1105	54-55	11.045508875641286
1105	56-57	11.006779952705912
1105	58-59	10.855845637889242
1105	60-61	10.777224164056364
1105	62-63	10.88341986866227
1105	64-65	10.587094905231666
1105	66-67	10.623897244527914
1105	68-69	10.459806023069671
1105	70-71	10.320395661657393
1105	72-73	10.135124212473908
1105	74-75	10.11564166706724
1105	76-77	9.883715880284601
1105	78-79	9.802226857359134
1105	80-81	9.513962384344502
1105	82-83	9.47969557912231
1105	84-85	9.145970656582467
1105	86-87	8.92750723573279
1105	88-89	8.846899983915247
1105	90-91	8.646131205828226
1105	92-93	8.6556564504945
1105	94-95	8.456803527034408
1105	96-97	8.213431499569973
1105	98-99	7.7175288701394695
1105	100-101	6.955017741786834
1106	1	8.01117279542121
1106	2	9.587496457234103
1106	3	8.871411513252944
1106	4	10.983761342697775
1106	5	11.737125709505822
1106	6	10.889238751825832
1106	7	10.1144898974355
1106	8	9.50967538733288
1106	9	9.08049338396178
1106	10-11	8.636852549590305
1106	12-13	8.345156506119618
1106	14-15	9.258667255515459
1106	16-17	10.608336492284554
1106	18-19	11.970605058838604
1106	20-21	12.1373947322265
1106	22-23	12.379901984054236
1106	24-25	12.08765436831247
1106	26-27	12.396721470750112
1106	28-29	12.118792468780274
1106	30-31	11.698937281376143
1106	32-33	11.830553175466562
1106	34-35	11.592055285524136
1106	36-37	12.008863935478598
1106	38-39	11.593705486484662
1106	40-41	11.414494594566047
1106	42-43	11.13994859117524
1106	44-45	10.816997479556889
1106	46-47	10.834731972966143
1106	48-49	10.95312413834819
1106	50-51	13.012988171112074
1106	52-53	11.678466358729082
1106	54-55	11.148226586044355
1106	56-57	10.976333019590626
1106	58-59	10.918972947641738
1106	60-61	10.816345879021029
1106	62-63	10.924615326841067
1106	64-65	10.792078026992186
1106	66-67	10.450718925108411
1106	68-69	10.194759476679092
1106	70-71	10.116311860934491
1106	72-73	9.925669620965078
1106	74-75	9.751229311429377
1106	76-77	9.737844924799049
1106	78-79	9.443858672326744
1106	80-81	9.471263104745649
1106	82-83	9.185825904572095
1106	84-85	8.812100498765236
1106	86-87	8.894292487978731
1106	88-89	8.738225452031568
1106	90-91	8.452335993282393
1106	92-93	8.406781296140682
1106	94-95	8.261045944880403
1106	96-97	7.908634296741694
1106	98-99	7.454242915489916
1106	100-101	6.85299438054807
1107	1	7.440121417447568
1107	2	8.742347755285952
1107	3	8.46619898675345
1107	4	10.557672351812016
1107	5	11.516596306639027
1107	6	10.371495944222882
1107	7	9.970443563114056
1107	8	8.828071454006622
1107	9	6.0232364173325195
1107	10-11	7.701711258469533
1107	12-13	7.597633037558904
1107	14-15	8.349818456712928
1107	16-17	9.72825798718333
1107	18-19	11.271256483546178
1107	20-21	11.754231957043803
1107	22-23	11.342137666426055
1107	24-25	11.550045266032896
1107	26-27	11.634010321434072
1107	28-29	11.686923963837092
1107	30-31	11.006299653337589
1107	32-33	11.194681892450852
1107	34-35	11.656512551069948
1107	36-37	12.042946262828327
1107	38-39	11.680097785094159
1107	40-41	11.289831552977358
1107	42-43	10.918804958842586
1107	44-45	10.48577046553897
1107	46-47	10.797365571049827
1107	48-49	10.978318411522682
1107	50-51	12.935613743151197
1107	52-53	11.255396165268678
1107	54-55	11.190167748703264
1107	56-57	10.814596368675026
1107	58-59	10.851235699570356
1107	60-61	10.61857062294392
1107	62-63	10.786019732247748
1107	64-65	10.738598014213729
1107	66-67	10.808972317833721
1107	68-69	10.55795168069189
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2201	9	9.758429115542292
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2201	98-99	8.121854533541951
2201	100-101	7.3238690388492795
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2202	3	9.00143165867577
2202	4	11.432463613057955
2202	5	12.04272454512692
2202	6	10.954546633502542
2202	7	10.37549752594752
2202	8	9.882300733095857
2202	9	9.72319599490713
2202	10-11	8.956813899401322
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2202	30-31	11.79095456620848
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2202	50-51	13.69205772385445
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2202	56-57	11.581068507021328
2202	58-59	11.15720184523688
2202	60-61	11.181543598454127
2202	62-63	11.190258758001026
2202	64-65	10.830641174540553
2202	66-67	10.807384688421216
2202	68-69	10.978089917655655
2202	70-71	10.543379122172244
2202	72-73	10.39878234463653
2202	74-75	10.195294154729066
2202	76-77	9.853553860394609
2202	78-79	9.57484371879713
2202	80-81	9.486839731939991
2202	82-83	9.20124794183991
2202	84-85	8.988065257676695
2202	86-87	9.107646891747585
2202	88-89	8.714047695816362
2202	90-91	8.534791579813088
2202	92-93	8.583972621886023
2202	94-95	8.115606334176283
2202	96-97	7.886907372943158
2202	98-99	7.799079977436614
2202	100-101	6.768643646056406
2203	1	8.442802183605295
2203	2	10.103734898960173
2203	3	9.273786340872181
2203	4	11.579276205304218
2203	5	12.124334508429985
2203	6	11.076252011966805
2203	7	10.61513125822275
2203	8	10.019094052778005
2203	9	9.724732669062575
2203	10-11	9.039477152050974
2203	12-13	8.788056666285893
2203	14-15	9.722039454010332
2203	16-17	11.08005587968199
2203	18-19	12.315894896654864
2203	20-21	12.564018991129696
2203	22-23	12.781650969837273
2203	24-25	12.652537363448623
2203	26-27	12.755545573072443
2203	28-29	12.522509551881047
2203	30-31	12.171353148593848
2203	32-33	12.158543396386861
2203	34-35	11.975311969509601
2203	36-37	12.521567534751451
2203	38-39	12.164183464825552
2203	40-41	11.941166587622117
2203	42-43	11.760047880353767
2203	44-45	11.47308976487227
2203	46-47	11.431248610619509
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2312	96-97	-13.346494582795392
2312	98-99	-12.711985385404352
2312	100-101	-11.537326503197326
2313	1	-20.733698325041704
2313	2	-19.213660828405036
2313	3	-20.065989854448265
2313	4	-21.496512197544206
2313	5	-20.720528156880288
2313	6	-21.94663710298742
2313	7	-22.358419097830147
2313	8	-22.99006159320368
2313	9	-24.710669162066623
2313	10-11	-25.346840243676393
2313	12-13	-25.37420172638959
2313	14-15	-25.44149055616261
2313	16-17	-24.05473557504843
2313	18-19	-22.770320769672704
2313	20-21	-22.397323836947617
2313	22-23	-22.014890230569648
2313	24-25	-21.896038424954227
2313	26-27	-21.665614142085243
2313	28-29	-21.570318525433297
2313	30-31	-21.758453565546535
2313	32-33	-21.702850296390352
2313	34-35	-21.438981011161818
2313	36-37	-20.883824123437766
2313	38-39	-20.920760584004558
2313	40-41	-20.940318661614914
2313	42-43	-20.881355985363427
2313	44-45	-20.52334970613485
2313	46-47	-20.215241725087512
2313	48-49	-20.113419785902206
2313	50-51	-17.345770377020482
2313	52-53	-19.589024435589693
2313	54-55	-20.55929577061004
2313	56-57	-20.618880026174764
2313	58-59	-20.357723527897452
2313	60-61	-20.098435814824313
2313	62-63	-19.714784988782295
2313	64-65	-19.45963159952018
2313	66-67	-19.192100643539668
2313	68-69	-18.80497750385747
2313	70-71	-18.531505077518954
2313	72-73	-18.175329852995997
2313	74-75	-17.776913771158735
2313	76-77	-17.444953602291957
2313	78-79	-17.163190249293454
2313	80-81	-16.796227689573133
2313	82-83	-16.3983403237288
2313	84-85	-16.09505363064034
2313	86-87	-15.664097832905014
2313	88-89	-15.308855031924246
2313	90-91	-14.911682944118976
2313	92-93	-14.431724753306977
2313	94-95	-13.919648426503079
2313	96-97	-13.346494582795392
2313	98-99	-12.711985385404352
2313	100-101	-11.537326503197326
2314	1	-20.733698325041704
2314	2	-19.213660828405036
2314	3	-20.065989854448265
2314	4	-21.496512197544206
2314	5	-20.720528156880288
2314	6	-21.94663710298742
2314	7	-22.358419097830147
2314	8	-22.99006159320368
2314	9	-24.710669162066623
2314	10-11	-25.346840243676393
2314	12-13	-25.37420172638959
2314	14-15	-25.44149055616261
2314	16-17	-24.05473557504843
2314	18-19	-22.770320769672704
2314	20-21	-22.397323836947617
2314	22-23	-22.014890230569648
2314	24-25	-21.896038424954227
2314	26-27	-21.665614142085243
2314	28-29	-21.570318525433297
2314	30-31	-21.758453565546535
2314	32-33	-21.702850296390352
2314	34-35	-21.438981011161818
2314	36-37	-20.883824123437766
2314	38-39	-20.920760584004558
2314	40-41	-20.940318661614914
2314	42-43	-20.881355985363427
2314	44-45	-20.52334970613485
2314	46-47	-20.215241725087512
2314	48-49	-20.113419785902206
2314	50-51	-17.345770377020482
2314	52-53	-19.589024435589693
2314	54-55	-20.55929577061004
2314	56-57	-20.618880026174764
2314	58-59	-20.357723527897452
2314	60-61	-20.098435814824313
2314	62-63	-19.714784988782295
2314	64-65	-19.45963159952018
2314	66-67	-19.192100643539668
2314	68-69	-18.80497750385747
2314	70-71	-18.531505077518954
2314	72-73	-18.175329852995997
2314	74-75	-17.776913771158735
2314	76-77	-17.444953602291957
2314	78-79	-17.163190249293454
2314	80-81	-16.796227689573133
2314	82-83	-16.3983403237288
2314	84-85	-16.09505363064034
2314	86-87	-15.664097832905014
2314	88-89	-15.308855031924246
2314	90-91	-14.911682944118976
2314	92-93	-14.431724753306977
2314	94-95	-13.919648426503079
2314	96-97	-13.346494582795392
2314	98-99	-12.711985385404352
2314	100-101	-11.537326503197326
2315	1	-20.733698325041704
2315	2	-19.213660828405036
2315	3	-20.065989854448265
2315	4	-21.496512197544206
2315	5	-20.720528156880288
2315	6	-21.94663710298742
2315	7	-22.358419097830147
2315	8	-22.99006159320368
2315	9	-24.710669162066623
2315	10-11	-25.346840243676393
2315	12-13	-25.37420172638959
2315	14-15	-25.44149055616261
2315	16-17	-24.05473557504843
2315	18-19	-22.770320769672704
2315	20-21	-22.397323836947617
2315	22-23	-22.014890230569648
2315	24-25	-21.896038424954227
2315	26-27	-21.665614142085243
2315	28-29	-21.570318525433297
2315	30-31	-21.758453565546535
2315	32-33	-21.702850296390352
2315	34-35	-21.438981011161818
2315	36-37	-20.883824123437766
2315	38-39	-20.920760584004558
2315	40-41	-20.940318661614914
2315	42-43	-20.881355985363427
2315	44-45	-20.52334970613485
2315	46-47	-20.215241725087512
2315	48-49	-20.113419785902206
2315	50-51	-17.345770377020482
2315	52-53	-19.589024435589693
2315	54-55	-20.55929577061004
2315	56-57	-20.618880026174764
2315	58-59	-20.357723527897452
2315	60-61	-20.098435814824313
2315	62-63	-19.714784988782295
2315	64-65	-19.45963159952018
2315	66-67	-19.192100643539668
2315	68-69	-18.80497750385747
2315	70-71	-18.531505077518954
2315	72-73	-18.175329852995997
2315	74-75	-17.776913771158735
2315	76-77	-17.444953602291957
2315	78-79	-17.163190249293454
2315	80-81	-16.796227689573133
2315	82-83	-16.3983403237288
2315	84-85	-16.09505363064034
2315	86-87	-15.664097832905014
2315	88-89	-15.308855031924246
2315	90-91	-14.911682944118976
2315	92-93	-14.431724753306977
2315	94-95	-13.919648426503079
2315	96-97	-13.346494582795392
2315	98-99	-12.711985385404352
2315	100-101	-11.537326503197326
2316	1	-20.733698325041704
2316	2	-19.213660828405036
2316	3	-20.065989854448265
2316	4	-21.496512197544206
2316	5	-20.720528156880288
2316	6	-21.94663710298742
2316	7	-22.358419097830147
2316	8	-22.99006159320368
2316	9	-24.710669162066623
2316	10-11	-25.346840243676393
2316	12-13	-25.37420172638959
2316	14-15	-25.44149055616261
2316	16-17	-24.05473557504843
2316	18-19	-22.770320769672704
2316	20-21	-22.397323836947617
2316	22-23	-22.014890230569648
2316	24-25	-21.896038424954227
2316	26-27	-21.665614142085243
2316	28-29	-21.570318525433297
2316	30-31	-21.758453565546535
2316	32-33	-21.702850296390352
2316	34-35	-21.438981011161818
2316	36-37	-20.883824123437766
2316	38-39	-20.920760584004558
2316	40-41	-20.940318661614914
2316	42-43	-20.881355985363427
2316	44-45	-20.52334970613485
2316	46-47	-20.215241725087512
2316	48-49	-20.113419785902206
2316	50-51	-17.345770377020482
2316	52-53	-19.589024435589693
2316	54-55	-20.55929577061004
2316	56-57	-20.618880026174764
2316	58-59	-20.357723527897452
2316	60-61	-20.098435814824313
2316	62-63	-19.714784988782295
2316	64-65	-19.45963159952018
2316	66-67	-19.192100643539668
2316	68-69	-18.80497750385747
2316	70-71	-18.531505077518954
2316	72-73	-18.175329852995997
2316	74-75	-17.776913771158735
2316	76-77	-17.444953602291957
2316	78-79	-17.163190249293454
2316	80-81	-16.796227689573133
2316	82-83	-16.3983403237288
2316	84-85	-16.09505363064034
2316	86-87	-15.664097832905014
2316	88-89	-15.308855031924246
2316	90-91	-14.911682944118976
2316	92-93	-14.431724753306977
2316	94-95	-13.919648426503079
2316	96-97	-13.346494582795392
2316	98-99	-12.711985385404352
2316	100-101	-11.537326503197326
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
2	349620.0
3	13947.0
4	64594.0
5	32346.0
6	10828.0
7	11452.0
8	12782.0
9	10077.0
10	8866.0
11	10142.0
12	11393.0
13	12706.0
14	13877.0
15	14993.0
16	16692.0
17	17559.0
18	15846.0
19	15387.0
20	16560.0
21	17686.0
22	19558.0
23	21584.0
24	23520.0
25	26753.0
26	30184.0
27	34173.0
28	39040.0
29	44435.0
30	52193.0
31	61783.0
32	72753.0
33	87872.0
34	105217.0
35	124311.0
36	135858.0
37	108737.0
38	37586.0
39	1360.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.3423050277411	21.232524884091355	14.299100560743236	23.126069527424313
2	14.66768198290615	20.658209470410725	42.45856887295856	22.215539673724567
3	18.394372211000285	26.342564185250044	30.508838955001277	24.754224648748394
4	11.767477100311805	17.169423695227735	40.51990143987152	30.543197764588935
5	12.116993994810246	38.46562927871881	36.3605207311576	13.056855995313352
6	29.02528785304955	39.39504694301479	18.2488144523034	13.330850751632259
7	25.435908745212686	33.340759912784264	23.683512918094397	17.539818423908653
8	23.984765813286106	37.346813350231386	20.856512319416456	17.81190851706605
9	24.950670213760212	16.819712959221146	21.933753947370153	36.29586287964848
10-11	23.511404806351596	27.67779047089565	29.37848949006416	19.432315232688584
12-13	24.023659631020298	25.21363579789537	29.70136761883732	21.061336952247004
14-15	21.400168348412898	26.84614242304496	27.467686777026806	24.286002451515333
16-17	20.49706165300318	29.92329973230718	28.24002425966204	21.339614355027607
18-19	20.190745710437938	28.772145100745227	30.589493843641396	20.44761534517544
20-21	21.052962537925833	28.114916425114345	30.548699648383664	20.283421388576155
22-23	20.716361706834586	27.867434650319495	30.316384470613883	21.09981917223204
24-25	20.53068569040147	28.176506380208643	30.61394208025264	20.67886584913725
26-27	20.5187975017625	28.342082027575522	30.426418184244813	20.712702286417166
28-29	20.401798078028317	28.462792945621796	30.413616576137898	20.721792400211992
30-31	20.813105575187688	28.188917167749576	30.434844360839662	20.563132896223067
32-33	20.624495887219013	28.477082594617343	30.13704074595054	20.76138077221311
34-35	20.693977197859464	28.383575337901064	30.375225001103136	20.547222463136336
36-37	20.703936772202955	28.22795301349585	30.367292819075743	20.700817395225457
38-39	20.616200539685728	28.655753760277324	30.360763534269093	20.36728216576785
40-41	20.812628521660248	28.350749070451307	30.307460974201334	20.52916143368711
42-43	20.620017025903476	28.448582888081443	30.09076246554488	20.840637620470204
44-45	20.643321564021953	28.79916976077499	29.883868960322683	20.67363971488037
46-47	20.735994854531068	28.622461093909944	29.667594963413997	20.973949088144998
48-49	20.686488266800993	28.79831207656609	29.624576379802146	20.89062327683077
50-51	21.113872648292737	28.930401632988094	29.070914748817245	20.88481096990192
52-53	20.569879957072942	29.131886261753902	29.276083965444233	21.02214981572892
54-55	20.553649125933422	29.05789347971119	29.36597134190765	21.02248605244774
56-57	20.672066722396778	29.110950967109623	29.29366886704525	20.92331344344835
58-59	20.528982547549923	29.051635636662898	29.573165045158483	20.846216770628693
60-61	20.618163432747703	29.275153572044893	29.297481690942504	20.8092013042649
62-63	20.48814973117437	28.97561517206011	29.379731638871466	21.156503457894054
64-65	20.315492201005778	29.02267769488024	29.522190315718543	21.13963978839544
66-67	20.47454109778219	28.98933271623831	29.151282836049297	21.384843349930204
68-69	20.33051221613366	29.020280847762773	29.296286798432945	21.35292013767062
70-71	20.479213864050763	28.66299917956296	29.441524202892744	21.416262753493534
72-73	20.592349908839346	28.957769941971844	29.25755430306059	21.192325846128217
74-75	20.386637241541685	29.18743658661851	28.968004303944937	21.457921867894875
76-77	20.333241132617655	29.15802822078523	29.18498864310625	21.32374200349086
78-79	20.285674498904925	29.07080510587666	29.103467784974583	21.540052610243833
80-81	20.318276335848573	29.098638878517363	29.038537314622282	21.54454747101179
82-83	20.597563838389107	28.882674486628684	29.200217607583472	21.31954406739873
84-85	20.629461251078858	28.988367900596394	28.98714570157998	21.39502514674476
86-87	20.33234182370654	29.141120802482128	29.085108135565296	21.441429238246034
88-89	20.506967600072933	29.086674814639636	28.981786211391153	21.424571373896278
90-91	20.493175002963575	28.950258818639625	29.22451730360297	21.33204887479383
92-93	20.61063842398706	28.89072890086401	28.86892195381464	21.62971072133429
94-95	20.23328346880487	28.958827921771206	29.010550455893057	21.79733815353087
96-97	20.290268243582393	28.92511436568797	29.002245336505744	21.782372054223895
98-99	20.178456924630872	28.867618853063682	28.753383439447074	22.200540782858376
100-101	20.41131650366863	28.8947510460357	28.682903041190976	22.011029409104687
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	94142.0
1	85487.5
2	57244.0
3	29425.0
4	18224.0
5	14398.5
6	13680.5
7	14001.0
8	14461.5
9	14305.5
10	12923.0
11	10885.0
12	8716.5
13	6734.5
14	5270.0
15	4533.5
16	4435.0
17	4719.5
18	5303.0
19	6082.5
20	7088.0
21	8056.0
22	8990.0
23	10258.0
24	11652.5
25	13410.5
26	15679.5
27	18114.0
28	20595.5
29	23240.0
30	26288.5
31	29484.5
32	33132.0
33	37261.0
34	41212.0
35	44838.0
36	48522.5
37	51845.5
38	53091.0
39	52847.5
40	52174.0
41	50713.0
42	49259.0
43	49031.5
44	49338.0
45	49995.0
46	49491.0
47	47643.0
48	46151.0
49	44220.0
50	41705.0
51	38966.5
52	35584.0
53	31890.5
54	28278.5
55	24201.5
56	19837.5
57	15600.0
58	12372.0
59	10026.0
60	7796.5
61	5681.0
62	3890.5
63	2581.0
64	1633.0
65	997.0
66	629.5
67	395.0
68	242.5
69	153.0
70	88.0
71	53.5
72	40.5
73	26.5
74	20.0
75	17.0
76	13.5
77	6.5
78	4.0
79	4.5
80	2.5
81	1.5
82	1.0
83	1.0
84	1.5
85	1.5
86	0.5
87	0.5
88	1.0
89	0.5
90	0.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	fail
#Base	N-Count
1	20.724767789141392
2	27.41449418225985
3	21.395201464556674
4	27.512189969899136
5	30.489769813468527
6	23.42216902251404
7	22.30849571957495
8	18.561554213827623
9	10.849454605197534
10-11	14.30075633555716
12-13	14.434655306964272
14-15	18.406795871546176
16-17	21.44155562205519
18-19	25.212759715303328
20-21	27.21725430829622
22-23	26.795871546175192
24-25	25.457057860550265
26-27	26.800360271553213
28-29	23.93969265433294
30-31	25.637457679827726
32-33	24.707059327453983
34-35	22.873840412610676
36-37	25.699654397483968
38-39	25.829299348107988
40-41	23.02906816408198
42-43	23.802595832819918
44-45	22.682878886561404
46-47	25.740669025447843
48-49	21.25637369665605
50-51	26.943530074460032
52-53	20.52958158038339
54-55	21.63571499821038
56-57	23.667464662289426
58-59	22.92650225609792
60-61	22.476837590287925
62-63	22.312808416506773
64-65	20.933097455215428
66-67	21.396844396721175
68-69	22.319556173610987
70-71	20.90091358763576
72-73	20.74979316657572
74-75	18.255880817006695
76-77	20.344458331132977
78-79	19.520029103369772
80-81	20.481731181092197
82-83	21.264529681329837
84-85	18.38526172496142
86-87	19.862316416999654
88-89	18.745122545136628
90-91	19.813527199328746
92-93	19.816871739806487
94-95	18.944210717785328
96-97	17.878769209104192
98-99	17.277896108011053
100-101	18.90234528566483
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1704270.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.57857633380917
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.60496512481363	58.866930241561064
2	10.12698563789137	14.092424864748276
3	2.4962151267230333	5.21049284220923
4	1.0122341287787782	2.817192383876841
5	0.4920249873903212	1.7117199071639475
6	0.29042402283658847	1.2124374025264502
7	0.20159043609975402	0.981846288243283
8	0.13383533348609666	0.7449657573698546
9	0.09796763382951587	0.6134803639784672
>10	0.5033492514168532	6.282815763240129
>50	0.027774792549182	1.3147353409379055
>100	0.011921866102779174	1.3687503750103194
>500	3.5582904102016236E-4	0.1427303202027291
>1k	2.6687178076512173E-4	0.29913947855151335
>5k	0.0	0.0
>10k+	8.895726025504059E-5	4.340338670379955
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	70124	4.114606253703932	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2102	0.12333726463529839	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	5.867614873230181E-5	0.0	0.0	0.0	0.0
12-13	5.867614873230181E-5	0.0	0.0	0.0	0.0
14-15	5.867614873230181E-5	0.0	0.0	0.0	0.0
16-17	5.867614873230181E-5	0.0	0.0	0.0	0.0
18-19	5.867614873230181E-5	0.0	0.0	5.867614873230181E-5	0.0
20-21	5.867614873230181E-5	0.0	0.0	8.801422309845272E-5	0.0
22-23	5.867614873230181E-5	0.0	0.0	1.1735229746460362E-4	0.0
24-25	5.867614873230181E-5	0.0	0.0	1.1735229746460362E-4	0.0
26-27	5.867614873230181E-5	0.0	0.0	2.3470459492920724E-4	0.0
28-29	5.867614873230181E-5	0.0	0.0	7.041137847876217E-4	0.0
30-31	5.867614873230181E-5	0.0	0.0	0.002141679428729016	0.0
32-33	5.867614873230181E-5	0.0	0.0	0.006689080955482407	0.0
34-35	5.867614873230181E-5	0.0	0.0	0.012087286638854172	0.0
36-37	5.867614873230181E-5	0.0	0.0	0.02294237415433001	0.0
38-39	5.867614873230181E-5	0.0	0.0	0.038931624683882254	0.0
40-41	5.867614873230181E-5	0.0	0.0	0.05389404261061921	0.0
42-43	5.867614873230181E-5	0.0	0.0	0.07346253821284186	0.0
44-45	5.867614873230181E-5	0.0	0.0	0.09672763118519953	0.0
46-47	5.867614873230181E-5	0.0	0.0	0.12424674494064908	0.0
48-49	5.867614873230181E-5	0.0	0.0	0.157281416676935	0.0
50-51	5.867614873230181E-5	0.0	0.0	0.19324989584983598	0.0
52-53	5.867614873230181E-5	0.0	0.0	0.235438046788361	0.0
54-55	5.867614873230181E-5	0.0	0.0	0.28164551391504866	0.0
56-57	5.867614873230181E-5	0.0	0.0	0.3404976910935474	0.0
58-59	5.867614873230181E-5	0.0	0.0	0.4077112194663991	0.0
60-61	5.867614873230181E-5	0.0	0.0	0.4784159786888228	0.0
62-63	5.867614873230181E-5	0.0	0.0	0.5517318265298339	0.0
64-65	5.867614873230181E-5	0.0	0.0	0.6289789763359093	0.0
66-67	5.867614873230181E-5	0.0	0.0	0.7177266512935156	0.0
68-69	5.867614873230181E-5	0.0	0.0	0.8232850428629266	0.0
70-71	5.867614873230181E-5	0.0	0.0	0.9357085438340169	0.0
72-73	5.867614873230181E-5	0.0	0.0	1.0551438445786172	0.0
74-75	5.867614873230181E-5	0.0	0.0	1.1782757426933526	0.0
76-77	5.867614873230181E-5	0.0	0.0	1.3188637950559476	0.0
78-79	5.867614873230181E-5	0.0	0.0	1.475411759873729	0.0
80-81	5.867614873230181E-5	0.0	0.0	1.6489464697495113	0.0
82-83	5.867614873230181E-5	0.0	0.0	1.832016053794293	0.0
84-85	5.867614873230181E-5	0.0	0.0	2.0174913599370994	0.0
86-87	5.867614873230181E-5	0.0	0.0	2.2075140676066587	2.9338074366150905E-5
88-89	5.867614873230181E-5	0.0	0.0	2.4273735969065937	5.867614873230181E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1595	0.0	58.85656	1
GTATCAA	3260	0.0	46.950626	1
ATCAACG	3855	0.0	39.18866	3
CAACGCA	3995	0.0	37.554436	5
TCAACGC	4060	0.0	37.45236	4
TATCAAC	4095	0.0	36.626328	2
AACGCAG	4425	0.0	34.16676	6
TATACCG	195	0.0	33.451496	5
GTGGTAT	745	0.0	30.817257	1
ACGCAGA	4905	0.0	30.272364	7
CGCAGAG	4915	0.0	29.665285	8
TGGTATC	800	0.0	28.058384	2
GCAGAGT	5460	0.0	25.862257	9
GTACATG	3770	0.0	25.442175	1
TACATGG	3740	0.0	24.962004	2
ACATGGG	3650	0.0	24.751297	3
GTATAGG	490	0.0	23.948013	1
ATACCGA	170	3.5774356E-6	22.027014	6
GAGTACT	3680	0.0	21.771353	12-13
AGAGTAC	5040	0.0	21.769405	10-11
>>END_MODULE