FastQCFastQC Report
Sat 18 Jun 2016
SRR1931013_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931013_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1704270
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53450.31362401497415315No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49590.2909750215634847No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31050.18218944181379712No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27700.060.4037251
GTATCAA52600.040.7560121
ATCAACG68250.030.8533213
GTGGTAT12800.030.8225771
CAACGCA68800.030.6844165
TCAACGC70100.030.174694
TATCAAC70600.029.7598822
AACGCAG72200.029.2385856
TGGTATC13100.027.5775642
CGCAGAG82400.025.2162488
ACGCAGA82950.025.106247
ACGTGCG1252.2767638E-422.769959
CGTAGAC1503.0177895E-522.1823543
GCAGAGT91500.022.137459
GGCGTGC5000.020.8730668
GAGTACT66250.020.8366512-13
ACATGGG48400.020.4276713
AGAGTAC86600.020.10337610-11
CAGAGTA90300.020.0151110-11
TAACGCC1200.00474496419.8056744
Produced by FastQC (version 0.11.5)