FastQCFastQC Report
Sat 18 Jun 2016
SRR1931012_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931012_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2680188
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1287204.802648172441635No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21250.059.2588421
GTATCAA41400.047.7442131
ATCAACG52300.037.5134163
TCAACGC52750.037.479534
CAACGCA53350.036.9543765
TATCAAC54000.035.641832
AACGCAG59650.033.1157886
ACGCAGA67000.029.0279317
CGCAGAG68200.028.2536938
GTACATG50850.027.5043031
TACATGG51750.026.8217352
ACATGGG51800.025.2503453
GCAGAGT70950.024.7010129
GAGTACT43200.021.86018612-13
AGAGTAC63550.021.04825810-11
CAGAGTA69350.020.405110-11
GTACTTT49800.019.81293314-15
AGTACTT48100.019.28356212-13
GTGGTAT11850.019.1626991
GAGTACA37350.018.9300731
Produced by FastQC (version 0.11.5)