##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931012_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2680188 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 20.33424968696226 28.0 2.0 31.0 2.0 33.0 2 18.978792905572295 26.0 2.0 31.0 2.0 33.0 3 19.636966138196275 26.0 2.0 31.0 2.0 33.0 4 21.018215513240115 28.0 2.0 35.0 2.0 37.0 5 20.31072596399954 28.0 2.0 35.0 2.0 37.0 6 21.342635665856275 28.0 2.0 35.0 2.0 37.0 7 21.558169053812644 28.0 2.0 35.0 2.0 37.0 8 22.01999747778887 30.0 2.0 35.0 2.0 37.0 9 23.31183372211203 31.0 2.0 37.0 2.0 39.0 10-11 23.675426499932094 32.0 2.0 37.0 2.0 39.0 12-13 23.585999564209676 32.0 2.0 37.0 2.0 39.0 14-15 23.488885667721817 32.0 2.0 38.0 2.0 40.0 16-17 22.2554121203438 29.5 2.0 37.5 2.0 40.0 18-19 21.1531539951675 27.0 2.0 37.0 2.0 40.0 20-21 20.792561566576673 26.5 2.0 37.0 2.0 39.5 22-23 20.397756612595835 25.0 2.0 37.0 2.0 39.0 24-25 20.22181298476077 25.0 2.0 36.5 2.0 39.0 26-27 19.957194234135812 24.0 2.0 36.0 2.0 39.0 28-29 19.755964880075577 24.0 2.0 36.0 2.0 39.0 30-31 19.740554580499577 24.0 2.0 36.0 2.0 39.0 32-33 19.597550433029326 24.0 2.0 36.0 2.0 39.0 34-35 19.280546924320234 22.5 2.0 35.5 2.0 39.0 36-37 18.81318269464679 18.5 2.0 35.0 2.0 39.0 38-39 18.69084687342828 18.5 2.0 35.0 2.0 38.0 40-41 18.553768989339552 18.0 2.0 35.0 2.0 38.0 42-43 18.415654797350037 17.0 2.0 35.0 2.0 38.0 44-45 18.024094018777788 15.0 2.0 34.0 2.0 38.0 46-47 17.68473069799581 15.0 2.0 34.0 2.0 38.0 48-49 17.438618858080105 11.5 2.0 34.0 2.0 38.0 50-51 15.374945526209356 7.0 2.0 31.5 2.0 37.5 52-53 17.16423381494134 12.5 2.0 33.5 2.0 38.0 54-55 17.858073575435753 13.5 2.0 35.0 2.0 38.5 56-57 17.815506225682675 11.0 2.0 35.0 2.0 39.0 58-59 17.54189426264128 8.5 2.0 34.5 2.0 39.0 60-61 17.25944952369013 8.0 2.0 34.0 2.0 38.0 62-63 16.828052733614207 6.5 2.0 34.0 2.0 38.0 64-65 16.480383652191563 2.0 2.0 33.5 2.0 38.0 66-67 16.131084647793365 2.0 2.0 33.0 2.0 38.0 68-69 15.702235440200464 2.0 2.0 32.0 2.0 37.0 70-71 15.359986127838795 2.0 2.0 32.0 2.0 36.5 72-73 14.969991470747575 2.0 2.0 31.0 2.0 36.0 74-75 14.52588754967935 2.0 2.0 31.0 2.0 36.0 76-77 14.12640475220395 2.0 2.0 30.5 2.0 35.0 78-79 13.762300629657322 2.0 2.0 30.0 2.0 35.0 80-81 13.346614864330412 2.0 2.0 29.5 2.0 34.0 82-83 12.898168897107219 2.0 2.0 29.0 2.0 34.0 84-85 12.509787746232726 2.0 2.0 29.0 2.0 34.0 86-87 12.036009227710892 2.0 2.0 28.5 2.0 34.0 88-89 11.629970919950392 2.0 2.0 27.0 2.0 34.0 90-91 11.166177708429409 2.0 2.0 26.0 2.0 34.0 92-93 10.637266117152976 2.0 2.0 25.5 2.0 33.0 94-95 10.092205099045291 2.0 2.0 24.5 2.0 32.5 96-97 9.483373740946531 2.0 2.0 19.5 2.0 32.0 98-99 8.84888541400827 2.0 2.0 6.5 2.0 32.0 100-101 7.995429984762263 2.0 2.0 2.0 2.0 31.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.836239947735411 1101 2 9.521546134704003 1101 3 8.903687835409826 1101 4 10.82352672443821 1101 5 11.618736712452652 1101 6 10.755211947574942 1101 7 10.496116053853669 1101 8 9.90215123336262 1101 9 9.767651767911165 1101 10-11 9.128212084228164 1101 12-13 9.09801983602706 1101 14-15 9.56919938876575 1101 16-17 10.604714615399256 1101 18-19 11.54847596862664 1101 20-21 11.567078396793956 1101 22-23 11.813825423471588 1101 24-25 11.678266556457682 1101 26-27 12.084797656338164 1101 28-29 11.813197650952265 1101 30-31 11.375125527748743 1101 32-33 11.188721850697203 1101 34-35 11.13069197465704 1101 36-37 11.406448176952196 1101 38-39 11.216686820792962 1101 40-41 10.909321253185535 1101 42-43 10.634241138087901 1101 44-45 10.647798178212753 1101 46-47 10.485539747260894 1101 48-49 10.678066366902698 1101 50-51 12.274752469279447 1101 52-53 11.554146421778185 1101 54-55 11.203187285125313 1101 56-57 11.359446035347272 1101 58-59 10.99460415200586 1101 60-61 10.606241607359717 1101 62-63 10.711742628944908 1101 64-65 10.448961411428765 1101 66-67 10.06114108478188 1101 68-69 10.016870016576188 1101 70-71 9.758488099508982 1101 72-73 9.565694465510354 1101 74-75 9.494799341916972 1101 76-77 8.964364979959592 1101 78-79 8.385683386052028 1101 80-81 8.382150747516905 1101 82-83 8.08513122378738 1101 84-85 7.925523875656735 1101 86-87 7.5778719657502105 1101 88-89 7.16218911997295 1101 90-91 7.163465603107015 1101 92-93 7.001228028824308 1101 94-95 6.5096112003229205 1101 96-97 6.4187068776843805 1101 98-99 6.172524350457044 1101 100-101 5.429900418419891 1102 1 7.6214857975690755 1102 2 8.830581664328623 1102 3 8.284997355495271 1102 4 10.30856087636657 1102 5 10.917808842321275 1102 6 10.22604938005474 1102 7 9.71284210361289 1102 8 9.239073620799154 1102 9 9.038108131393148 1102 10-11 8.21335345769813 1102 12-13 8.470704789196237 1102 14-15 8.831105720103618 1102 16-17 9.901897600020515 1102 18-19 10.747715394744006 1102 20-21 10.952632728014347 1102 22-23 10.965067887798636 1102 24-25 10.63742876327428 1102 26-27 10.850377897569079 1102 28-29 10.625215768488015 1102 30-31 10.698171040883143 1102 32-33 10.409636770611424 1102 34-35 10.380269105537543 1102 36-37 10.33121721492056 1102 38-39 10.459048602681555 1102 40-41 10.203157435013349 1102 42-43 9.635746596842242 1102 44-45 9.624535428936234 1102 46-47 9.663866264466456 1102 48-49 9.371531742229166 1102 50-51 11.105008623106448 1102 52-53 10.38876014651856 1102 54-55 10.072472925993477 1102 56-57 9.762644039922144 1102 58-59 9.950944890509792 1102 60-61 9.924061865878866 1102 62-63 9.819198308686065 1102 64-65 9.387604211433619 1102 66-67 9.437937420671638 1102 68-69 9.168262986877831 1102 70-71 8.775185245098536 1102 72-73 8.56315589416478 1102 74-75 8.047060107642851 1102 76-77 7.9675368574633705 1102 78-79 7.730959493491907 1102 80-81 7.346465669218686 1102 82-83 7.355234385019342 1102 84-85 7.078112833785108 1102 86-87 6.712628679146617 1102 88-89 6.630293983215211 1102 90-91 6.3366271957698554 1102 92-93 5.718705092224511 1102 94-95 5.440381803681101 1102 96-97 5.246985386197306 1102 98-99 4.948071023907916 1102 100-101 4.349446436220134 1103 1 7.792258866579189 1103 2 9.105134127260492 1103 3 8.466535689698727 1103 4 10.441338089221585 1103 5 11.19178193097736 1103 6 10.304299014876133 1103 7 9.983251934360414 1103 8 9.565737328442832 1103 9 9.370786750572353 1103 10-11 8.81303130013379 1103 12-13 8.772512021582013 1103 14-15 9.386496220184565 1103 16-17 10.329821666518495 1103 18-19 11.238263650769838 1103 20-21 11.548707747736113 1103 22-23 11.58698001391122 1103 24-25 11.630750875968186 1103 26-27 11.354451026062836 1103 28-29 11.102465474859553 1103 30-31 10.965293506234381 1103 32-33 11.15168901980239 1103 34-35 11.089328248403177 1103 36-37 11.38331875791765 1103 38-39 11.042800398256723 1103 40-41 10.75075192516702 1103 42-43 10.20586945390021 1103 44-45 10.383375434320683 1103 46-47 10.317778890217355 1103 48-49 9.957443988400478 1103 50-51 11.503065202900322 1103 52-53 10.744697414018482 1103 54-55 10.537894953615474 1103 56-57 10.458730853290863 1103 58-59 10.303388100613155 1103 60-61 10.251546429541214 1103 62-63 10.285145744795724 1103 64-65 9.655852454665308 1103 66-67 9.636904657981034 1103 68-69 9.482680022967603 1103 70-71 9.244130738439823 1103 72-73 8.918427608876154 1103 74-75 8.777767717364698 1103 76-77 8.509910536590072 1103 78-79 7.994737065310366 1103 80-81 7.57346002342708 1103 82-83 7.510376627316026 1103 84-85 7.193620388085909 1103 86-87 6.853868136869272 1103 88-89 6.633203284916103 1103 90-91 6.3895302671508265 1103 92-93 6.31754729868549 1103 94-95 5.815201639807013 1103 96-97 5.501162353356394 1103 98-99 5.114109883519861 1103 100-101 4.477799363985412 1104 1 7.934325229505937 1104 2 9.348928828222707 1104 3 8.823681474723323 1104 4 10.720964521734949 1104 5 11.478567569547423 1104 6 10.416650022687662 1104 7 10.169589779129247 1104 8 9.684502370172911 1104 9 9.56578442998995 1104 10-11 9.074636526094164 1104 12-13 8.829050194538059 1104 14-15 9.316812855168191 1104 16-17 10.119011851578879 1104 18-19 11.105974439942685 1104 20-21 11.280553910239174 1104 22-23 11.629589036106399 1104 24-25 11.405471763310704 1104 26-27 11.296862897924374 1104 28-29 11.192723201282359 1104 30-31 11.200828873188314 1104 32-33 11.36845014790956 1104 34-35 11.06933370202892 1104 36-37 11.15085245117265 1104 38-39 10.771599097614 1104 40-41 10.546281435937711 1104 42-43 10.031337930195082 1104 44-45 10.21859028322719 1104 46-47 10.177813539546428 1104 48-49 10.283290560219307 1104 50-51 11.769302264312818 1104 52-53 10.924679845468741 1104 54-55 10.379518988120235 1104 56-57 10.243719748433595 1104 58-59 10.224729907047347 1104 60-61 9.935565454937745 1104 62-63 9.984534019000858 1104 64-65 9.895091717949818 1104 66-67 9.526236972285595 1104 68-69 9.391820884449476 1104 70-71 9.2621188882635 1104 72-73 8.929428668013763 1104 74-75 8.70908927691948 1104 76-77 8.353533034030379 1104 78-79 8.104903317151814 1104 80-81 7.940730937721851 1104 82-83 7.49629446062813 1104 84-85 7.24488285396218 1104 86-87 7.040446781546306 1104 88-89 6.666073206980087 1104 90-91 6.43140263849808 1104 92-93 6.038362944832151 1104 94-95 5.7844437489086165 1104 96-97 5.176441987575604 1104 98-99 4.774888632479859 1104 100-101 3.9620763347137515 1105 1 7.9532042479306995 1105 2 9.023223270454086 1105 3 8.6564121297904 1105 4 10.664098512395874 1105 5 10.837186046674212 1105 6 10.602343006242862 1105 7 10.064317121525974 1105 8 9.613224053611752 1105 9 9.605251475493656 1105 10-11 8.915712767516819 1105 12-13 8.770499687271364 1105 14-15 9.520940363198445 1105 16-17 10.420579962182511 1105 18-19 11.057476982596967 1105 20-21 11.255426763478777 1105 22-23 11.735593152784755 1105 24-25 11.710322555491768 1105 26-27 11.851136739908423 1105 28-29 11.487294868961289 1105 30-31 11.293676769592079 1105 32-33 11.117424208959104 1105 34-35 11.131630557969899 1105 36-37 11.219961884473417 1105 38-39 11.211429477767641 1105 40-41 10.660839655725749 1105 42-43 10.667447539916889 1105 44-45 10.64098068446146 1105 46-47 10.537112347355922 1105 48-49 10.383846553393944 1105 50-51 11.853323981518875 1105 52-53 10.837484375444308 1105 54-55 10.583915271367033 1105 56-57 10.51922347546866 1105 58-59 10.39785085434971 1105 60-61 10.304062457184585 1105 62-63 10.167728102378344 1105 64-65 9.987774008928803 1105 66-67 9.758488888617066 1105 68-69 9.66506210989029 1105 70-71 9.495899669943578 1105 72-73 9.045441940045464 1105 74-75 8.903482093548904 1105 76-77 8.50014910699902 1105 78-79 8.19115621278865 1105 80-81 8.089464203708038 1105 82-83 7.92603744775108 1105 84-85 7.763141378778835 1105 86-87 7.686653546450655 1105 88-89 7.428161630444535 1105 90-91 6.759187885765657 1105 92-93 6.406264947435766 1105 94-95 5.963100117230814 1105 96-97 5.719440684644374 1105 98-99 5.128209702490361 1105 100-101 4.574591595977028 1106 1 8.02601081694283 1106 2 9.17823209391564 1106 3 8.867639468195502 1106 4 10.52788562043056 1106 5 11.150130539616246 1106 6 10.463587165293731 1106 7 9.77657448974681 1106 8 9.303970713224658 1106 9 8.911527937159487 1106 10-11 8.645910548264663 1106 12-13 8.485559356299003 1106 14-15 9.084476596800993 1106 16-17 10.184308439401672 1106 18-19 11.402327517611539 1106 20-21 11.455608075016993 1106 22-23 11.608784732160235 1106 24-25 11.335596125723466 1106 26-27 11.38188049413706 1106 28-29 11.483187962087573 1106 30-31 11.231189680175458 1106 32-33 11.352922773436372 1106 34-35 11.019306738808744 1106 36-37 11.477404819024205 1106 38-39 11.114576025028292 1106 40-41 10.879369085953872 1106 42-43 10.750072926161305 1106 44-45 10.336552817131555 1106 46-47 10.062303947120057 1106 48-49 10.290941123517797 1106 50-51 11.985445392242559 1106 52-53 11.353020200200543 1106 54-55 10.812534003932715 1106 56-57 10.563531725740557 1106 58-59 10.199356570418278 1106 60-61 10.10923719337945 1106 62-63 10.053193790138867 1106 64-65 9.902487432877216 1106 66-67 9.809216348012782 1106 68-69 9.553707422832101 1106 70-71 8.977328147860243 1106 72-73 8.711899935915149 1106 74-75 8.575396470828212 1106 76-77 8.468818105663564 1106 78-79 8.146361116154443 1106 80-81 7.655743517103767 1106 82-83 7.60961735869555 1106 84-85 7.273806727872964 1106 86-87 7.191867611300546 1106 88-89 6.800530420017992 1106 90-91 6.507470006894698 1106 92-93 6.226156004915161 1106 94-95 5.979627273586631 1106 96-97 5.4034767918908795 1106 98-99 4.787109541044353 1106 100-101 4.428279945091004 1107 1 7.259311949249906 1107 2 8.113957514668758 1107 3 8.05761482423343 1107 4 9.72287444662226 1107 5 10.651615727144168 1107 6 9.442160957311636 1107 7 9.379372873745876 1107 8 7.96898348925582 1107 9 5.5073396900643665 1107 10-11 7.429664086724269 1107 12-13 7.285394358657978 1107 14-15 7.957203606207663 1107 16-17 8.960230200302007 1107 18-19 10.290897223178298 1107 20-21 10.474440456410747 1107 22-23 9.976179262056561 1107 24-25 10.128232345980571 1107 26-27 10.029780212208461 1107 28-29 10.18425942905569 1107 30-31 9.989210384339358 1107 32-33 10.211819911772398 1107 34-35 10.284705366116047 1107 36-37 10.641647471240592 1107 38-39 10.3303793962722 1107 40-41 9.846878733214329 1107 42-43 9.656013506656564 1107 44-45 9.031247815482363 1107 46-47 9.312737251853456 1107 48-49 9.278738656825244 1107 50-51 10.904872501018355 1107 52-53 9.717433525684552 1107 54-55 9.539446403050082 1107 56-57 9.319439204937755 1107 58-59 9.592163098615313 1107 60-61 9.346807505912068 1107 62-63 9.454236043939321 1107 64-65 9.341239528292455 1107 66-67 9.269700613349237 1107 68-69 8.861681534668303 1107 70-71 8.601186368359508 1107 72-73 8.143124519545964 1107 74-75 8.193618598413162 1107 76-77 8.09575636476231 1107 78-79 7.7509312089685505 1107 80-81 7.211981670048727 1107 82-83 7.276097773478172 1107 84-85 7.1494838888384304 1107 86-87 6.667539788152551 1107 88-89 6.4911723033761675 1107 90-91 6.1392930233741065 1107 92-93 5.877171446272758 1107 94-95 5.567956789245619 1107 96-97 4.984239588276013 1107 98-99 4.507801779605421 1107 100-101 4.207118782346916 1108 1 6.509839311099391 1108 2 7.892260791425006 1108 3 7.689305918219674 1108 4 8.916107855554284 1108 5 10.748229129247925 1108 6 9.308264901449892 1108 7 8.538828705412786 1108 8 5.812305198274011 1108 9 6.663883346277277 1108 10-11 6.032034776379444 1108 12-13 4.9268497757514105 1108 14-15 7.147805286449579 1108 16-17 8.377953368862709 1108 18-19 8.164736315831405 1108 20-21 8.007818701870761 1108 22-23 9.142496894435563 1108 24-25 8.874457999949907 1108 26-27 7.240528588462343 1108 28-29 8.872083822640366 1108 30-31 9.268839940583163 1108 32-33 10.002928733245678 1108 34-35 9.472234071264886 1108 36-37 9.160782628290782 1108 38-39 8.864470443923764 1108 40-41 9.084153207275033 1108 42-43 8.233954082425964 1108 44-45 8.067581421133568 1108 46-47 8.97391336022784 1108 48-49 9.40587045867538 1108 50-51 10.67644302321836 1108 52-53 10.028745691383879 1108 54-55 9.362156545061822 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6.322993597881915 1109 18-19 5.471131966845764 1109 20-21 7.612645623475611 1109 22-23 8.818710152118253 1109 24-25 8.380313599175071 1109 26-27 8.602671062866381 1109 28-29 9.209271055545962 1109 30-31 6.798121959123321 1109 32-33 5.616648359751093 1109 34-35 7.403008990609386 1109 36-37 5.766117593017864 1109 38-39 6.323735209963083 1109 40-41 7.2373038913048795 1109 42-43 5.943588057346577 1109 44-45 6.620632495557011 1109 46-47 6.103936258735743 1109 48-49 6.726204750562243 1109 50-51 8.445674795708653 1109 52-53 8.909073744871751 1109 54-55 8.843276124370913 1109 56-57 7.8466106470088555 1109 58-59 7.693122451216677 1109 60-61 7.87258432633071 1109 62-63 8.403965403018827 1109 64-65 8.317654654170575 1109 66-67 7.962525583038641 1109 68-69 7.480620158686179 1109 70-71 6.916026153094856 1109 72-73 6.517148711560608 1109 74-75 5.79392289300837 1109 76-77 5.576919036076605 1109 78-79 6.483772292304854 1109 80-81 6.589258307712312 1109 82-83 6.385326049399671 1109 84-85 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-14.449228984351912 2313 68-69 -14.014036856136297 2313 70-71 -13.67359811439911 2313 72-73 -13.27102777930461 2313 74-75 -12.82362278089718 2313 76-77 -12.405500819145734 2313 78-79 -12.02708603241076 2313 80-81 -11.600159134548976 2313 82-83 -11.165173778245967 2313 84-85 -10.762154042196054 2313 86-87 -10.283250441732507 2313 88-89 -9.868921087428433 2313 90-91 -9.39590812605118 2313 92-93 -8.856844358324942 2313 94-95 -8.281360739490014 2313 96-97 -7.666083687586054 2313 98-99 -7.006206841084234 2313 100-101 -6.143096734894465 2314 1 -18.57042943006044 2314 2 -17.27145915199791 2314 3 -17.910057589559674 2314 4 -19.38021463383751 2314 5 -18.684231974005257 2314 6 -19.68643099671135 2314 7 -19.894693218208147 2314 8 -20.32843030384763 2314 9 -21.61299076970575 2314 10-11 -21.967965223620556 2314 12-13 -21.861516560882254 2314 14-15 -21.780942945516475 2314 16-17 -20.600653420387648 2314 18-19 -19.55644473084469 2314 20-21 -19.175703221750172 2314 22-23 -18.800042002311255 2314 24-25 -18.625718803444716 2314 26-27 -18.368028695837513 2314 28-29 -18.15284545600178 2314 30-31 -18.129144500718112 2314 32-33 -17.96418583535404 2314 34-35 -17.662313312044873 2314 36-37 -17.208029252897337 2314 38-39 -17.06940508648642 2314 40-41 -16.92557097942935 2314 42-43 -16.777714941619298 2314 44-45 -16.372128621299247 2314 46-47 -16.043943782629306 2314 48-49 -15.78879007880694 2314 50-51 -13.686003935763255 2314 52-53 -15.500957278913152 2314 54-55 -16.19636537855911 2314 56-57 -16.147874013453052 2314 58-59 -15.87892167149963 2314 60-61 -15.603803126271842 2314 62-63 -15.172559933072176 2314 64-65 -14.807646965960416 2314 66-67 -14.449228984351912 2314 68-69 -14.014036856136297 2314 70-71 -13.67359811439911 2314 72-73 -13.27102777930461 2314 74-75 -12.82362278089718 2314 76-77 -12.405500819145734 2314 78-79 -12.02708603241076 2314 80-81 -11.600159134548976 2314 82-83 -11.165173778245967 2314 84-85 -10.762154042196054 2314 86-87 -10.283250441732507 2314 88-89 -9.868921087428433 2314 90-91 -9.39590812605118 2314 92-93 -8.856844358324942 2314 94-95 -8.281360739490014 2314 96-97 -7.666083687586054 2314 98-99 -7.006206841084234 2314 100-101 -6.143096734894465 2315 1 -18.57042943006044 2315 2 -17.27145915199791 2315 3 -17.910057589559674 2315 4 -19.38021463383751 2315 5 -18.684231974005257 2315 6 -19.68643099671135 2315 7 -19.894693218208147 2315 8 -20.32843030384763 2315 9 -21.61299076970575 2315 10-11 -21.967965223620556 2315 12-13 -21.861516560882254 2315 14-15 -21.780942945516475 2315 16-17 -20.600653420387648 2315 18-19 -19.55644473084469 2315 20-21 -19.175703221750172 2315 22-23 -18.800042002311255 2315 24-25 -18.625718803444716 2315 26-27 -18.368028695837513 2315 28-29 -18.15284545600178 2315 30-31 -18.129144500718112 2315 32-33 -17.96418583535404 2315 34-35 -17.662313312044873 2315 36-37 -17.208029252897337 2315 38-39 -17.06940508648642 2315 40-41 -16.92557097942935 2315 42-43 -16.777714941619298 2315 44-45 -16.372128621299247 2315 46-47 -16.043943782629306 2315 48-49 -15.78879007880694 2315 50-51 -13.686003935763255 2315 52-53 -15.500957278913152 2315 54-55 -16.19636537855911 2315 56-57 -16.147874013453052 2315 58-59 -15.87892167149963 2315 60-61 -15.603803126271842 2315 62-63 -15.172559933072176 2315 64-65 -14.807646965960416 2315 66-67 -14.449228984351912 2315 68-69 -14.014036856136297 2315 70-71 -13.67359811439911 2315 72-73 -13.27102777930461 2315 74-75 -12.82362278089718 2315 76-77 -12.405500819145734 2315 78-79 -12.02708603241076 2315 80-81 -11.600159134548976 2315 82-83 -11.165173778245967 2315 84-85 -10.762154042196054 2315 86-87 -10.283250441732507 2315 88-89 -9.868921087428433 2315 90-91 -9.39590812605118 2315 92-93 -8.856844358324942 2315 94-95 -8.281360739490014 2315 96-97 -7.666083687586054 2315 98-99 -7.006206841084234 2315 100-101 -6.143096734894465 2316 1 -18.57042943006044 2316 2 -17.27145915199791 2316 3 -17.910057589559674 2316 4 -19.38021463383751 2316 5 -18.684231974005257 2316 6 -19.68643099671135 2316 7 -19.894693218208147 2316 8 -20.32843030384763 2316 9 -21.61299076970575 2316 10-11 -21.967965223620556 2316 12-13 -21.861516560882254 2316 14-15 -21.780942945516475 2316 16-17 -20.600653420387648 2316 18-19 -19.55644473084469 2316 20-21 -19.175703221750172 2316 22-23 -18.800042002311255 2316 24-25 -18.625718803444716 2316 26-27 -18.368028695837513 2316 28-29 -18.15284545600178 2316 30-31 -18.129144500718112 2316 32-33 -17.96418583535404 2316 34-35 -17.662313312044873 2316 36-37 -17.208029252897337 2316 38-39 -17.06940508648642 2316 40-41 -16.92557097942935 2316 42-43 -16.777714941619298 2316 44-45 -16.372128621299247 2316 46-47 -16.043943782629306 2316 48-49 -15.78879007880694 2316 50-51 -13.686003935763255 2316 52-53 -15.500957278913152 2316 54-55 -16.19636537855911 2316 56-57 -16.147874013453052 2316 58-59 -15.87892167149963 2316 60-61 -15.603803126271842 2316 62-63 -15.172559933072176 2316 64-65 -14.807646965960416 2316 66-67 -14.449228984351912 2316 68-69 -14.014036856136297 2316 70-71 -13.67359811439911 2316 72-73 -13.27102777930461 2316 74-75 -12.82362278089718 2316 76-77 -12.405500819145734 2316 78-79 -12.02708603241076 2316 80-81 -11.600159134548976 2316 82-83 -11.165173778245967 2316 84-85 -10.762154042196054 2316 86-87 -10.283250441732507 2316 88-89 -9.868921087428433 2316 90-91 -9.39590812605118 2316 92-93 -8.856844358324942 2316 94-95 -8.281360739490014 2316 96-97 -7.666083687586054 2316 98-99 -7.006206841084234 2316 100-101 -6.143096734894465 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 744549.0 3 30818.0 4 111773.0 5 61431.0 6 35015.0 7 34517.0 8 33731.0 9 32652.0 10 34134.0 11 35748.0 12 36668.0 13 37280.0 14 38072.0 15 38737.0 16 40500.0 17 40215.0 18 39795.0 19 41065.0 20 42917.0 21 45248.0 22 47755.0 23 50950.0 24 53941.0 25 57595.0 26 61149.0 27 65180.0 28 70167.0 29 75240.0 30 80877.0 31 87365.0 32 93767.0 33 98964.0 34 99656.0 35 90917.0 36 64132.0 37 24976.0 38 2681.0 39 11.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.04329512789867 20.96900911057709 14.089673926386695 22.89802183513754 2 14.800679787745965 21.054258907244463 42.21167266543425 21.93338863957532 3 18.477998095831225 27.031585921885302 30.390643109625003 24.099772872658466 4 11.955959970158263 17.549157276543074 40.50972175909073 29.98516099420793 5 12.266877363956407 38.50942768927585 36.13156101081872 13.092133935949018 6 28.730607815279306 39.376299674013886 18.60800757736161 13.285084933345198 7 25.646628106348235 33.261173476564096 23.71993976483684 17.372258652250828 8 24.08848287485905 37.236710046701674 21.011559797935973 17.6632472805033 9 25.489719460295778 16.871308589387603 21.817739863183363 35.82123208713326 10-11 23.685692197733275 27.463682662714124 29.55582160156456 19.29480353798804 12-13 24.393342516275794 25.06573132265779 29.631523216841575 20.90940294422484 14-15 21.85500422337798 26.651539703660237 27.30511543781404 24.18834063514774 16-17 21.009228616115113 29.626794943221324 28.058193608603105 21.30578283206046 18-19 20.792606824931287 28.574743866196663 30.188385510152365 20.444263798719685 20-21 21.668014235765256 27.879977116238365 30.12152581965041 20.330482828345968 22-23 21.38906402440591 27.72450682785085 29.778772060651253 21.107657087091987 24-25 21.070336948829645 28.032075873912245 30.136285746512687 20.76130143074543 26-27 21.11085844045429 28.131363654532354 29.870433968154853 20.887343936858503 28-29 21.039201664282928 28.207237690724885 29.961891140541514 20.791669504450677 30-31 21.40897577388034 28.04845649016965 29.78575189813691 20.756815837813107 32-33 21.20155340780668 28.15491508919387 29.761821959509106 20.881709543490345 34-35 21.28220063212974 28.138228614839612 29.9134528665215 20.66611788650915 36-37 21.384746122795836 27.95525281495645 29.76657106437221 20.893429997875504 38-39 21.359109314974404 28.210616552322687 29.80078323629403 20.629490896408882 40-41 21.49046242318444 28.062580595565912 29.765441130427746 20.681515850821903 42-43 21.335237183240654 28.088206211903344 29.534915560492227 21.041641044363775 44-45 21.32611623669853 28.450851218137757 29.354656113937132 20.86837643122658 46-47 21.4642973027487 28.243128648871014 29.03614396997698 21.25643007840331 48-49 21.487811350948636 28.32925898902472 29.04767094557641 21.135258714450238 50-51 21.647093825006333 28.57165831085237 28.529926144518736 21.251321719622563 52-53 21.099711416073816 28.894888986736134 28.740607768842263 21.264791828347786 54-55 21.091813642059588 28.780543762195997 28.83844863810076 21.289193957643658 56-57 21.175534772071313 28.776455893351425 28.842070364002254 21.205938970575005 58-59 21.15189428837569 28.76877618230776 28.934128749510073 21.145200779806473 60-61 21.20776946764609 28.943041225127736 28.781185740541922 21.068003566684258 62-63 21.14684549407628 28.759920672443506 28.807224708037527 21.286009125442686 64-65 20.914809667514092 28.60963378825358 28.933436403709646 21.54212014052268 66-67 20.92117027296623 28.656655484933502 28.641737667191443 21.780436574908826 68-69 21.002556238895444 28.73224627380187 28.65141937731504 21.613778109987646 70-71 21.177788546492582 28.331805662495256 28.818594909832036 21.67181088118012 72-73 21.134888265395535 28.572178585081986 28.729262430587205 21.56367071893527 74-75 21.024691712189 28.816639018581082 28.410891409526034 21.747777859703884 76-77 21.041192654529997 28.652460422945335 28.558261988691292 21.748084933833375 78-79 20.88292108066295 28.815201872182268 28.497604709402953 21.804272337751826 80-81 20.93782320486726 28.73964364190465 28.308298950163113 22.014234203064976 82-83 21.179892072612567 28.536367193773565 28.561999078147167 21.7217416554667 84-85 21.254824985901326 28.54922247709918 28.274488827752293 21.921463709247206 86-87 20.991775674283513 28.674698853811098 28.41269613402432 21.920829337881074 88-89 21.21716854927551 28.487729939350494 28.296346414721484 21.998755096652513 90-91 21.182193898046584 28.399825113778178 28.52367043187559 21.894310556299647 92-93 21.210960158977056 28.546460771867245 28.17687899101888 22.065700078136814 94-95 20.955834133973877 28.42599623700942 28.341572318161884 22.276597310854815 96-97 20.990393434800467 28.450017698855568 28.24933608601573 22.310252780328234 98-99 20.865401839323432 28.465470485862078 28.029621310101817 22.639506364712673 100-101 21.220799462868378 28.467088698654774 27.826870968515454 22.48524086996139 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 175721.0 1 159716.5 2 106133.5 3 53509.0 4 32916.0 5 25718.0 6 23697.5 7 23868.0 8 24379.0 9 24158.5 10 22657.0 11 19382.5 12 15357.0 13 11465.0 14 8495.0 15 6719.0 16 6141.0 17 6570.0 18 7549.5 19 8883.5 20 10545.5 21 12202.5 22 13872.5 23 15647.5 24 17542.0 25 19513.5 26 21952.5 27 25155.0 28 28516.0 29 32425.0 30 37086.5 31 42100.5 32 47865.5 33 53830.0 34 59259.0 35 64433.5 36 69567.0 37 74438.5 38 76975.0 39 77457.0 40 77148.0 41 76597.5 42 76307.5 43 76533.5 44 77103.5 45 77501.5 46 76912.0 47 75192.0 48 73267.0 49 70696.5 50 66616.5 51 61311.0 52 55565.5 53 49790.5 54 43890.0 55 37260.0 56 30904.0 57 24999.0 58 19735.0 59 15629.5 60 12110.0 61 9100.0 62 6627.5 63 4618.0 64 3100.0 65 2078.5 66 1392.0 67 881.5 68 570.0 69 387.0 70 252.5 71 160.0 72 108.5 73 78.5 74 53.5 75 42.5 76 32.0 77 23.5 78 17.0 79 9.5 80 7.0 81 5.0 82 4.0 83 3.0 84 1.5 85 1.5 86 1.5 87 1.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 24.015255646245713 2 31.458390232326988 3 24.99309749913066 4 31.63416148419439 5 35.022505883915606 6 27.046237055012558 7 25.991796097885672 8 21.74597453611463 9 12.968381322504241 10-11 16.867678685226558 12-13 16.842773715873662 14-15 21.219649517123425 16-17 24.926497693445384 18-19 29.179259066901274 20-21 31.358080104828467 22-23 31.02840173898249 24-25 29.377174287773844 26-27 30.925218678689703 28-29 27.686714514056476 30-31 29.7819033590181 32-33 28.654892865724346 34-35 26.526945124744984 36-37 29.751196557853405 38-39 29.92637083667265 40-41 26.710999377655597 42-43 27.645803204849813 44-45 26.175514553456697 46-47 29.8000923815792 48-49 24.855066137151574 50-51 30.39788626767973 52-53 23.990817062086688 54-55 25.191348517342817 56-57 27.413487412077064 58-59 26.69954495729404 60-61 26.18771519012845 62-63 26.015730986035308 64-65 24.439871382156774 66-67 24.967278414797768 68-69 26.04945996325631 70-71 24.430133259308676 72-73 24.31372351491761 74-75 21.31348621813097 76-77 23.812583296395626 78-79 22.922403204551323 80-81 23.971844512399876 82-83 24.961719103286782 84-85 21.666390566631893 86-87 23.32849412056169 88-89 22.13572331493164 90-91 23.281725013319964 92-93 23.264748592262933 94-95 22.195401964339815 96-97 21.10465758372174 98-99 20.537272012261827 100-101 22.201353039413654 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2680188.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.1142688408812 #Duplication Level Percentage of deduplicated Percentage of total 1 88.30637730837128 66.33068965505291 2 7.695389159458323 11.560670603175105 3 1.973054346224536 4.446136037999367 4 0.7798070580448833 2.3429854800800003 5 0.3588056545898058 1.3475712200243515 6 0.22837796943918284 1.0292666516273596 7 0.1339615870847978 0.7043698666647034 8 0.09101555210982959 0.5469253319903177 9 0.07188087924586384 0.48593517193734603 >10 0.3387623062545517 4.507511166274004 >50 0.015710964128031284 0.7919203030351867 >100 0.006503211241355544 0.7894288666180185 >500 2.0228789001739534E-4 0.09397926250870063 >1k 1.0114394499582624E-4 0.1329536858307664 >5k 0.0 0.0 >10k+ 5.057197249791312E-5 4.889656697181785 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 128720 4.802648172441635 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 3.7310815509956765E-5 0.0 0.0 0.0 0.0 7 3.7310815509956765E-5 0.0 0.0 3.7310815509956765E-5 0.0 8 3.7310815509956765E-5 0.0 0.0 3.7310815509956765E-5 0.0 9 3.7310815509956765E-5 0.0 0.0 3.7310815509956765E-5 0.0 10-11 3.7310815509956765E-5 0.0 0.0 3.7310815509956765E-5 0.0 12-13 3.7310815509956765E-5 0.0 0.0 3.7310815509956765E-5 0.0 14-15 3.7310815509956765E-5 0.0 0.0 7.462163101991353E-5 0.0 16-17 5.5966223264935144E-5 0.0 0.0 1.1193244652987029E-4 0.0 18-19 7.462163101991353E-5 0.0 0.0 1.6789866979480543E-4 0.0 20-21 7.462163101991353E-5 0.0 0.0 2.052094853047622E-4 0.0 22-23 7.462163101991353E-5 0.0 0.0 2.2386489305974058E-4 0.0 24-25 7.462163101991353E-5 0.0 0.0 2.4252030081471898E-4 0.0 26-27 7.462163101991353E-5 0.0 0.0 3.171419318346325E-4 0.0 28-29 7.462163101991353E-5 0.0 0.0 5.223514171393947E-4 0.0 30-31 7.462163101991353E-5 0.0 0.0 0.0016976421057030328 0.0 32-33 7.462163101991353E-5 0.0 0.0 0.005130237132619056 0.0 34-35 7.462163101991353E-5 0.0 0.0 0.00886131868361473 0.0 36-37 7.462163101991353E-5 0.0 0.0 0.015857096591731627 0.0 38-39 9.327703877489192E-5 0.0 0.0 0.026994375021453722 0.0 40-41 1.1193244652987029E-4 0.0 0.0 0.0391763562854546 0.0 42-43 1.1193244652987029E-4 0.0 0.0 0.05154489162700527 0.0 44-45 1.1193244652987029E-4 0.0 0.0 0.06684232598608754 0.0 46-47 1.1193244652987029E-4 0.0 0.0 0.08631857168228498 0.0 48-49 1.1193244652987029E-4 0.0 0.0 0.11027211523967723 0.0 50-51 1.1193244652987029E-4 0.0 0.0 0.1355688481554279 0.0 52-53 1.1193244652987029E-4 0.0 0.0 0.16562271004869808 0.0 54-55 1.1193244652987029E-4 0.0 0.0 0.19638547743665744 0.0 56-57 1.1193244652987029E-4 0.0 0.0 0.2343865430335484 0.0 58-59 1.1193244652987029E-4 0.0 0.0 0.2785625485973372 0.0 60-61 1.1193244652987029E-4 0.0 0.0 0.32626442622681695 0.0 62-63 1.4924326203982706E-4 0.0 0.0 0.37458193231221093 0.0 64-65 1.6789866979480543E-4 0.0 0.0 0.4267797632106405 0.0 66-67 1.8655407754978383E-4 0.0 0.0 0.4873538721910553 0.0 68-69 1.8655407754978383E-4 0.0 0.0 0.5571250971946744 0.0 70-71 1.8655407754978383E-4 0.0 0.0 0.6343957961157949 1.8655407754978382E-5 72-73 2.052094853047622E-4 0.0 0.0 0.7173563944021837 3.7310815509956765E-5 74-75 2.2386489305974058E-4 0.0 0.0 0.8010072427755068 3.7310815509956765E-5 76-77 2.2386489305974058E-4 0.0 0.0 0.8915979028336818 3.7310815509956765E-5 78-79 2.2386489305974058E-4 0.0 0.0 0.9953779361746266 3.7310815509956765E-5 80-81 2.2386489305974058E-4 0.0 0.0 1.1120488562742614 3.7310815509956765E-5 82-83 2.2386489305974058E-4 0.0 0.0 1.2346521960399794 3.7310815509956765E-5 84-85 2.2386489305974058E-4 0.0 0.0 1.3541214273028608 3.7310815509956765E-5 86-87 2.2386489305974058E-4 0.0 0.0 1.4836272679379208 3.7310815509956765E-5 88-89 2.2386489305974058E-4 0.0 0.0 1.6305945702316404 3.7310815509956765E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2125 0.0 59.258842 1 GTATCAA 4140 0.0 47.744213 1 ATCAACG 5230 0.0 37.513416 3 TCAACGC 5275 0.0 37.47953 4 CAACGCA 5335 0.0 36.954376 5 TATCAAC 5400 0.0 35.64183 2 AACGCAG 5965 0.0 33.115788 6 ACGCAGA 6700 0.0 29.027931 7 CGCAGAG 6820 0.0 28.253693 8 GTACATG 5085 0.0 27.504303 1 TACATGG 5175 0.0 26.821735 2 ACATGGG 5180 0.0 25.250345 3 GCAGAGT 7095 0.0 24.701012 9 GAGTACT 4320 0.0 21.860186 12-13 AGAGTAC 6355 0.0 21.048258 10-11 CAGAGTA 6935 0.0 20.4051 10-11 GTACTTT 4980 0.0 19.812933 14-15 AGTACTT 4810 0.0 19.283562 12-13 GTGGTAT 1185 0.0 19.162699 1 GAGTACA 3735 0.0 18.930073 1 >>END_MODULE