FastQCFastQC Report
Sat 18 Jun 2016
SRR1931012_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931012_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2680188
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60660.22632740688339772No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53460.1994636197162289No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30230.1127905952865993No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36150.058.3678671
GTATCAA68050.040.853271
CAACGCA85200.031.9062825
ATCAACG85250.031.8291173
TCAACGC86750.031.3335384
TATCAAC89800.030.4826972
AACGCAG90750.030.111266
ACGCAGA104900.025.7781937
CGCAGAG105300.025.6352168
GTACATG87300.023.24411
TACATGG84950.022.936532
ACATGGG81700.022.4516453
GCAGAGT119800.021.9776449
GTGGTAT17000.021.2453421
GTATAGG8550.020.5652851
AGAGTAC110150.020.37143310-11
GAGTACT78050.020.2402712-13
CAGAGTA117050.019.67717610-11
GAGTACA63450.019.4733561
AGTACAT64400.018.3009242
Produced by FastQC (version 0.11.5)