##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931012_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2680188 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.350050817330725 31.0 31.0 34.0 30.0 34.0 2 31.518213274591186 31.0 31.0 34.0 30.0 34.0 3 31.58398366084767 31.0 31.0 34.0 30.0 34.0 4 35.35472735494674 37.0 35.0 37.0 33.0 37.0 5 35.231980741649465 37.0 35.0 37.0 33.0 37.0 6 35.254334397437795 37.0 35.0 37.0 32.0 37.0 7 35.13113632327285 37.0 35.0 37.0 32.0 37.0 8 35.137903012773734 37.0 35.0 37.0 32.0 37.0 9 36.641555368504 39.0 35.0 39.0 32.0 39.0 10-11 36.581514804185375 38.5 35.0 39.0 32.0 39.0 12-13 36.46690530664267 38.0 35.0 39.0 32.0 39.0 14-15 37.533687748769864 39.0 36.0 41.0 32.0 41.0 16-17 37.48938824440673 39.0 36.0 41.0 32.0 41.0 18-19 37.515812137059044 39.0 36.0 41.0 32.0 41.0 20-21 37.50057719831594 39.0 36.0 41.0 32.0 41.0 22-23 37.3979829026919 39.0 36.0 41.0 32.0 41.0 24-25 37.27533478994757 39.0 36.0 41.0 31.0 41.0 26-27 37.02960128170113 39.0 36.0 40.0 31.0 41.0 28-29 36.85487361334354 39.0 36.0 40.0 30.5 41.0 30-31 36.6349974330159 38.5 35.0 40.0 30.0 41.0 32-33 36.47921283880086 38.0 35.0 40.0 30.0 41.0 34-35 36.56969343195328 38.0 35.0 40.0 30.0 41.0 36-37 36.64404157469551 39.0 35.0 40.0 30.0 41.0 38-39 36.63131504207914 39.0 35.0 40.0 30.0 41.0 40-41 36.534676298826795 39.0 35.0 40.0 30.0 41.0 42-43 36.40134703237236 39.0 35.0 40.0 30.0 41.0 44-45 36.24180673146809 39.0 35.0 40.0 30.0 41.0 46-47 36.01008903106797 38.0 35.0 40.0 29.5 41.0 48-49 35.79529850144841 38.0 35.0 40.0 28.5 41.0 50-51 35.54122658559773 38.0 34.5 40.0 27.5 41.0 52-53 35.345611949609506 38.0 34.0 40.0 27.0 41.0 54-55 35.20586839430667 38.0 34.0 40.0 27.0 41.0 56-57 35.0408312775074 38.0 34.0 40.0 26.5 41.0 58-59 34.84450829568672 38.0 34.0 40.0 26.0 41.0 60-61 34.54218864497565 38.0 33.0 40.0 26.0 41.0 62-63 34.29713102215217 37.0 33.0 40.0 26.0 41.0 64-65 33.989112890588274 37.0 33.0 40.0 25.0 41.0 66-67 33.680259742973256 36.0 32.0 39.0 24.5 41.0 68-69 33.34006289857278 36.0 32.0 39.0 23.5 40.0 70-71 32.9688553564153 35.5 31.5 39.0 23.0 40.0 72-73 32.55284573320976 35.0 31.0 38.0 22.0 40.0 74-75 32.041981383395495 35.0 31.0 37.5 22.0 39.5 76-77 30.637239626473963 33.5 29.5 36.0 20.0 38.5 78-79 31.37679670232088 34.0 30.5 36.0 21.0 39.0 80-81 31.34725306582971 34.0 31.0 36.0 21.0 38.5 82-83 31.07667391242704 34.0 31.0 36.0 20.5 37.0 84-85 30.738007184570634 34.0 30.5 35.0 20.0 37.0 86-87 30.399634839048602 34.0 30.0 35.0 18.5 36.5 88-89 30.05531179156089 34.0 30.0 35.0 18.0 36.0 90-91 29.725979856636926 34.0 29.5 35.0 14.0 36.0 92-93 29.412991364784858 34.0 29.0 35.0 8.5 35.5 94-95 29.03651422959882 34.0 29.0 35.0 2.0 35.0 96-97 28.62717969784209 34.0 29.0 35.0 2.0 35.0 98-99 28.145896481888585 34.0 29.0 35.0 2.0 35.0 100-101 27.0190814972681 33.0 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3627828516149805 1101 2 0.3954714674721487 1101 3 0.32591525349284467 1101 4 0.23460197160747498 1101 5 0.2928960379827785 1101 6 0.3482924693778031 1101 7 0.3047733615271113 1101 8 0.38401714672910003 1101 9 0.45672342350544426 1101 10-11 0.47722183736063783 1101 12-13 0.42882249327678323 1101 14-15 0.400355049874058 1101 16-17 0.5683754782532731 1101 18-19 0.571307684392167 1101 20-21 0.4962617734724333 1101 22-23 0.4979643732609276 1101 24-25 0.4432381471959488 1101 26-27 0.3743538547935117 1101 28-29 0.4025700866664792 1101 30-31 0.5861388277407329 1101 32-33 0.5859685576536364 1101 34-35 0.5590507999431154 1101 36-37 0.5795784386076193 1101 38-39 0.5088194874991459 1101 40-41 0.6914681506958971 1101 42-43 0.6894746797508873 1101 44-45 0.6102174175413211 1101 46-47 0.6267984751690037 1101 48-49 0.7488158816792421 1101 50-51 0.5825079177296573 1101 52-53 0.46053380112725506 1101 54-55 0.446257234820564 1101 56-57 0.5911169610253779 1101 58-59 0.44629288583794136 1101 60-61 0.4709935070066962 1101 62-63 0.31606195588809527 1101 64-65 0.36309645170336324 1101 66-67 0.3534534900803763 1101 68-69 0.41477854229655264 1101 70-71 0.3636956708278589 1101 72-73 0.26571516132737116 1101 74-75 0.2584319826689523 1101 76-77 0.29355037660629435 1101 78-79 0.25043637507227245 1101 80-81 0.15121132112935953 1101 82-83 0.1619309617694462 1101 84-85 0.38027471741096974 1101 86-87 0.26771121529331765 1101 88-89 0.21387926302348603 1101 90-91 0.18808032655828555 1101 92-93 0.04968864264380457 1101 94-95 0.19419952873350965 1101 96-97 0.3735126753781195 1101 98-99 0.3594176725411842 1101 100-101 0.5560959794726337 1102 1 0.2913581106672041 1102 2 0.3439108019133421 1102 3 0.3091045501080423 1102 4 0.1620151585483427 1102 5 0.1782282013170331 1102 6 0.28825925910931005 1102 7 0.4042771227400408 1102 8 0.28458967592534634 1102 9 0.433028707717547 1102 10-11 0.49790116674559926 1102 12-13 0.46099254683328894 1102 14-15 0.546278811814787 1102 16-17 0.5050034453596624 1102 18-19 0.496022537017609 1102 20-21 0.486612712546723 1102 22-23 0.4776622664326453 1102 24-25 0.5346596737319231 1102 26-27 0.5328717587799545 1102 28-29 0.5460128940769238 1102 30-31 0.5457916553058908 1102 32-33 0.5392736401270852 1102 34-35 0.5456056527473052 1102 36-37 0.51231822830146 1102 38-39 0.566083738962547 1102 40-41 0.6168815872564295 1102 42-43 0.58660863421796 1102 44-45 0.8051837827278732 1102 46-47 0.6986717813782732 1102 48-49 0.6550828195865179 1102 50-51 0.5205560355909924 1102 52-53 0.5473696037374793 1102 54-55 0.7933469153137835 1102 56-57 0.7228650711619196 1102 58-59 0.6836299547412708 1102 60-61 0.4754563363402724 1102 62-63 0.3896621705226977 1102 64-65 0.38628929470535667 1102 66-67 0.40597823977442005 1102 68-69 0.32431950386214936 1102 70-71 0.4902637739035427 1102 72-73 0.3819281979857081 1102 74-75 0.5134033881298095 1102 76-77 0.46145926287485395 1102 78-79 0.5592357715161818 1102 80-81 0.5406349167633806 1102 82-83 0.5054392225204865 1102 84-85 0.5660035589522785 1102 86-87 0.5717994395654138 1102 88-89 0.5657821022396945 1102 90-91 0.4427894238863317 1102 92-93 0.42863435706997066 1102 94-95 0.46674877116016944 1102 96-97 0.5820792424310035 1102 98-99 0.7539504113413393 1102 100-101 0.9976742045938245 1103 1 0.3714854820543003 1103 2 0.38182707368990165 1103 3 0.4276795137417686 1103 4 0.25803014891029363 1103 5 0.26919344916856147 1103 6 0.3120527144538272 1103 7 0.41829003693504774 1103 8 0.3770066944307615 1103 9 0.4522071786424178 1103 10-11 0.4644813365619882 1103 12-13 0.47473631423952867 1103 14-15 0.4752995510490692 1103 16-17 0.6418698551734252 1103 18-19 0.5118818845894282 1103 20-21 0.4724742066229197 1103 22-23 0.5132521731324715 1103 24-25 0.5038683260849268 1103 26-27 0.5051013583477442 1103 28-29 0.49436725587690944 1103 30-31 0.4557184346458456 1103 32-33 0.5479371955751091 1103 34-35 0.5636334093531161 1103 36-37 0.6272964327929884 1103 38-39 0.44997376881350704 1103 40-41 0.586314743351565 1103 42-43 0.5337017731046814 1103 44-45 0.6870687410074652 1103 46-47 0.5635330347306677 1103 48-49 0.4173914911610339 1103 50-51 0.4290455968491429 1103 52-53 0.5165312634098171 1103 54-55 0.537752739859485 1103 56-57 0.5577161164913562 1103 58-59 0.6183302899961447 1103 60-61 0.4814689740364031 1103 62-63 0.3220415979153657 1103 64-65 0.3900944133300399 1103 66-67 0.3321611516592 1103 68-69 0.3333530129784208 1103 70-71 0.4153268956849203 1103 72-73 0.34144786912183633 1103 74-75 0.4113270241142075 1103 76-77 0.40191567094837666 1103 78-79 0.534217853661815 1103 80-81 0.4257096169349168 1103 82-83 0.46340381489991245 1103 84-85 0.49762435773083524 1103 86-87 0.4350817388475505 1103 88-89 0.3350305316230333 1103 90-91 0.39209099138025394 1103 92-93 0.2802491316900415 1103 94-95 0.40449377735410863 1103 96-97 0.45458423426640593 1103 98-99 0.41434332775560634 1103 100-101 0.6505269180118383 1104 1 0.3543277172710013 1104 2 0.355780134015788 1104 3 0.4608290957357575 1104 4 0.1972711458366092 1104 5 0.24724480933543447 1104 6 0.3406551596821359 1104 7 0.2806989674822731 1104 8 0.3686398158631121 1104 9 0.46018266553497966 1104 10-11 0.43743637750017683 1104 12-13 0.43992074654750013 1104 14-15 0.5269030889124693 1104 16-17 0.5433062214837108 1104 18-19 0.5586527490052688 1104 20-21 0.4677056632302268 1104 22-23 0.510819858989521 1104 24-25 0.4244979436508842 1104 26-27 0.6079053623551047 1104 28-29 0.5654067548485529 1104 30-31 0.7482597640053257 1104 32-33 0.536567664094747 1104 34-35 0.5716526780471725 1104 36-37 0.5758540648498425 1104 38-39 0.7906910205034805 1104 40-41 0.5772797515547765 1104 42-43 0.6334126222203977 1104 44-45 0.6971459747498372 1104 46-47 0.5894926311799935 1104 48-49 0.6865376916719939 1104 50-51 0.6402708246871427 1104 52-53 0.5244398287903635 1104 54-55 0.5202541234672537 1104 56-57 0.6787907951689363 1104 58-59 0.6822668528103222 1104 60-61 0.5253445831801002 1104 62-63 0.39158590429043016 1104 64-65 0.35303884820027776 1104 66-67 0.46108026566245286 1104 68-69 0.24404144360799052 1104 70-71 0.5084990502828717 1104 72-73 0.39936421385268517 1104 74-75 0.5073236191241719 1104 76-77 0.5952732223762034 1104 78-79 0.6761634493374622 1104 80-81 0.6518255790598388 1104 82-83 0.6041208755954486 1104 84-85 0.7195792122290676 1104 86-87 0.8285702389990952 1104 88-89 0.7246973800282532 1104 90-91 0.6136332244372475 1104 92-93 0.538179698160441 1104 94-95 0.8127494530843435 1104 96-97 0.7325319726057913 1104 98-99 0.8636586200483514 1104 100-101 1.0164988497633551 1105 1 0.2763615541534499 1105 2 0.33769107734586257 1105 3 0.332949822792731 1105 4 0.22051616376806749 1105 5 0.1677933945116834 1105 6 0.2962320312200859 1105 7 0.35344048311885246 1105 8 0.3332605134229354 1105 9 0.3753990088660686 1105 10-11 0.3877638099053513 1105 12-13 0.47080735220095704 1105 14-15 0.5770417816999327 1105 16-17 0.49352023032611214 1105 18-19 0.5380756780104008 1105 20-21 0.48756981621536966 1105 22-23 0.4887834516411971 1105 24-25 0.5445985373582758 1105 26-27 0.44780663399126297 1105 28-29 0.43515176744243433 1105 30-31 0.3986176908445316 1105 32-33 0.48289408910203946 1105 34-35 0.6069849255108366 1105 36-37 0.5460806254316495 1105 38-39 0.5420457122077025 1105 40-41 0.39904456038796354 1105 42-43 0.49044696195900883 1105 44-45 0.5644429346331492 1105 46-47 0.41926591608930863 1105 48-49 0.44674117705516636 1105 50-51 0.4792350702090502 1105 52-53 0.5638881927660861 1105 54-55 0.631083060788896 1105 56-57 0.6976795055142304 1105 58-59 0.5552728455073961 1105 60-61 0.5219326759955436 1105 62-63 0.47367569867001436 1105 64-65 0.2968995222073332 1105 66-67 0.457825606377007 1105 68-69 0.29154435539803814 1105 70-71 0.379040907299796 1105 72-73 0.37973891754679556 1105 74-75 0.5407918574364103 1105 76-77 0.6146428064888703 1105 78-79 0.6392568266374745 1105 80-81 0.5448621729425227 1105 82-83 0.6140267206943975 1105 84-85 0.6297541415623229 1105 86-87 0.6639524068766463 1105 88-89 0.6331071858920616 1105 90-91 0.5480798129654936 1105 92-93 0.5719712111844153 1105 94-95 0.6562819642806161 1105 96-97 0.6166062790154392 1105 98-99 0.6589217851880029 1105 100-101 0.7506347911690945 1106 1 0.2812777701680993 1106 2 0.2610289051271302 1106 3 0.2724001927664581 1106 4 0.13728361123104804 1106 5 0.23436519130540745 1106 6 0.15277298211506718 1106 7 0.2969023222512348 1106 8 0.32285277238469234 1106 9 0.43421479784556993 1106 10-11 0.37980465322331725 1106 12-13 0.4431622050511592 1106 14-15 0.4753283829690744 1106 16-17 0.46694884130562286 1106 18-19 0.4351069010993882 1106 20-21 0.22652446002813775 1106 22-23 0.31064858762015035 1106 24-25 0.3513708995617719 1106 26-27 0.5045434501729886 1106 28-29 0.3901637162711822 1106 30-31 0.40885092103626874 1106 32-33 0.48048312987452846 1106 34-35 0.45075251408481876 1106 36-37 0.4567035741579275 1106 38-39 0.3762749617815544 1106 40-41 0.4110378552010303 1106 42-43 0.4408115702472202 1106 44-45 0.426687881866755 1106 46-47 0.34165359200187595 1106 48-49 0.30767481142530073 1106 50-51 0.23828119740961284 1106 52-53 0.3075576474927928 1106 54-55 0.4660070674184382 1106 56-57 0.43032848626894094 1106 58-59 0.5082561225125701 1106 60-61 0.5204376639742847 1106 62-63 0.3187038296575011 1106 64-65 0.34569985067940223 1106 66-67 0.33494220430348065 1106 68-69 0.29215051567116035 1106 70-71 0.32006475881453866 1106 72-73 0.37774445038569127 1106 74-75 0.6196952398617057 1106 76-77 0.5759555224141835 1106 78-79 0.46520854789656596 1106 80-81 0.42868140921545717 1106 82-83 0.4389123966402302 1106 84-85 0.46586961060080156 1106 86-87 0.3709964479952106 1106 88-89 0.4700396098195512 1106 90-91 0.6114086724184027 1106 92-93 0.6873169787397231 1106 94-95 0.66802341888285 1106 96-97 0.7962372952131567 1106 98-99 0.7498771468962708 1106 100-101 1.07438265907847 1107 1 0.13804426523513413 1107 2 0.21486322797660407 1107 3 0.14781510064125314 1107 4 0.0778630626967427 1107 5 0.0885443834928239 1107 6 0.12952026616464707 1107 7 0.13675492818583024 1107 8 0.16387520976752512 1107 9 0.21921214661034583 1107 10-11 0.12477467808646736 1107 12-13 0.12707220814078113 1107 14-15 0.14122368631631588 1107 16-17 0.12986698493994453 1107 18-19 0.18916562648151114 1107 20-21 0.216461117425915 1107 22-23 0.20654924562751376 1107 24-25 0.18728586014813686 1107 26-27 0.23409279759336243 1107 28-29 0.22195125682255679 1107 30-31 0.23892403093518766 1107 32-33 0.2322839344304768 1107 34-35 0.25837225806317576 1107 36-37 0.23766202961508753 1107 38-39 0.27278180280347186 1107 40-41 0.2166637580823192 1107 42-43 0.22638554962603052 1107 44-45 0.3315429172304718 1107 46-47 0.2070838332016507 1107 48-49 0.2216447726170614 1107 50-51 0.2544601907543935 1107 52-53 0.16552430925363382 1107 54-55 0.24149956828478025 1107 56-57 0.4113623036092733 1107 58-59 0.3705968004981415 1107 60-61 0.3864218332817515 1107 62-63 -0.0020223713311722236 1107 64-65 0.0012050388544793122 1107 66-67 0.18101647124737497 1107 68-69 0.2532208057622185 1107 70-71 0.21298917042820165 1107 72-73 0.20238181046518378 1107 74-75 0.25389288631635765 1107 76-77 0.24044131325468854 1107 78-79 0.2450576466853498 1107 80-81 0.2494297908137959 1107 82-83 0.28290188198745625 1107 84-85 0.37346636502961417 1107 86-87 0.2898217446412943 1107 88-89 0.32645167179165213 1107 90-91 0.3918192015799029 1107 92-93 0.20155745203739883 1107 94-95 0.43616415667777275 1107 96-97 0.4258602561627569 1107 98-99 0.43269579875359554 1107 100-101 0.624506270311354 1108 1 0.042659849946314665 1108 2 0.1254789508789429 1108 3 0.04151358379005998 1108 4 -0.026904125844772864 1108 5 -0.04187453894129334 1108 6 0.04781829978574592 1108 7 0.06475631822630845 1108 8 0.10456884809687494 1108 9 0.11907639411883508 1108 10-11 0.05127247718904471 1108 12-13 0.004711557666873034 1108 14-15 0.03895409975174147 1108 16-17 0.010999217621346702 1108 18-19 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-3.0681307609509147 2316 76-77 -2.1841750717346358 2316 78-79 -1.8567636017813278 2316 80-81 -1.4910796419698116 2316 82-83 -1.2798646040876847 2316 84-85 -1.184501526024853 2316 86-87 -1.1271948890872991 2316 88-89 -1.1649854221613722 2316 90-91 -1.1396346737551575 2316 92-93 -1.0604956123395013 2316 94-95 -1.5242161645252637 2316 96-97 -1.2622809005316213 2316 98-99 -1.3678478214817424 2316 100-101 -1.7138532431137499 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 13.0 4 22.0 5 54.0 6 141.0 7 331.0 8 798.0 9 1573.0 10 2820.0 11 4498.0 12 6450.0 13 8559.0 14 10318.0 15 13170.0 16 15673.0 17 18954.0 18 16503.0 19 12552.0 20 12060.0 21 12777.0 22 14380.0 23 16849.0 24 20921.0 25 26906.0 26 34837.0 27 45274.0 28 57450.0 29 72685.0 30 89576.0 31 108693.0 32 131703.0 33 160709.0 34 199635.0 35 249374.0 36 327527.0 37 455686.0 38 445060.0 39 85633.0 40 19.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.13792207947272 22.077681727003533 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29.02385952030231 30.39331569277976 20.252870321037182 20-21 21.116783598762474 28.296298617858152 30.31600768304313 20.270910100336245 22-23 20.871937705676487 27.932172466028398 30.21968673745793 20.976203090837185 24-25 20.626470870811488 28.281948270120672 30.38145865611262 20.71012220295522 26-27 20.677964963365298 28.436772534251403 30.1912347594818 20.694027742901504 28-29 20.545186755006423 28.506267763482523 30.27566219837642 20.672883283134638 30-31 20.99940522698762 28.30210961584529 30.115626261653855 20.58285889551323 32-33 20.892961900909228 28.468781673983557 29.959837948623026 20.67841847648419 34-35 20.854919838787588 28.35318884469228 30.281453762633554 20.51043755388658 36-37 20.949107693030953 28.210839690697515 30.306293338139017 20.533759278132514 38-39 20.769432965640572 28.508815541037052 30.27046862311377 20.451282870208605 40-41 20.896685170688972 28.388612825160497 30.19536680573911 20.519335198411426 42-43 20.823736335414214 28.407244443723016 30.005136434560647 20.76388278630212 44-45 20.664944382741812 28.73604671099679 29.849343378476277 20.749665527785123 46-47 20.901491384449187 28.54340239393931 29.57141004723137 20.98369617438013 48-49 20.93533777070866 28.64916904161089 29.569618127626622 20.845875060053825 50-51 21.033950415385622 28.792774439533126 29.161397639450886 21.011877505630366 52-53 20.9298664100752 28.96492834912377 29.108047070987485 20.99715816981355 54-55 20.963655660218937 28.80012404364366 29.268888854059146 20.96733144207826 56-57 20.944583116370175 28.925686752526552 29.106508558905052 21.023221572198217 58-59 20.88941092176535 28.899426987026512 29.344944107445066 20.86621798376308 60-61 21.019458885378818 29.095197972431176 29.15864822020841 20.726694921981597 62-63 20.918142188659374 28.913748533952464 29.205181580459083 20.96292769692908 64-65 20.692110113017222 28.740260480998764 29.306440588583456 21.261188817400555 66-67 20.737149988694306 28.799784097703647 29.131735748606243 21.331330164995812 68-69 20.750132267043092 28.904888728304513 29.119003309621576 21.22597569503082 70-71 20.84957116419287 28.532058952278216 29.301938292596407 21.31643159093251 72-73 20.882902288648836 28.783658743855344 29.185299013192562 21.14813995430326 74-75 20.809223580708107 29.010595042753685 28.98564440629736 21.194536970240847 76-77 20.651229511567585 28.89144691831395 29.10047173585185 21.35685183426661 78-79 20.60312542093855 28.87803574820067 29.166316856235934 21.352521974624842 80-81 20.662065743920106 28.870560151213333 29.01092349153249 21.45645061333407 82-83 20.81740161511058 28.786450801212453 29.2593467323934 21.136800851283567 84-85 20.887004941444406 28.746919992179652 28.916665547342202 21.44940951903374 86-87 20.54445434424749 28.8801196035502 29.087306562076986 21.48811949012532 88-89 20.69595555979241 28.7435701745271 29.049388559112792 21.511085706567698 90-91 20.696157295459578 28.597776924966972 29.321892899193347 21.3841728803801 92-93 20.72490144477997 28.828558698011282 29.03102418315971 21.41551567404904 94-95 20.409737219544883 28.689608776959734 29.098454241396244 21.80219976209914 96-97 20.48233565748665 28.6086327915491 29.101340857682533 21.80769069328172 98-99 20.320757349857548 28.708676406281946 29.062494869762865 21.908071374097638 100-101 20.4013300559513 28.59510228386964 28.943547989917125 22.060019670261937 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 236.0 1 169.0 2 128.0 3 159.0 4 138.5 5 149.0 6 257.0 7 491.5 8 848.0 9 1141.5 10 1170.5 11 1102.5 12 1241.0 13 1493.5 14 1712.0 15 1980.5 16 2311.5 17 2721.5 18 3107.5 19 3467.5 20 3979.5 21 4733.0 22 5850.5 23 7416.5 24 9337.5 25 11795.5 26 15770.5 27 20883.5 28 25685.0 29 32145.5 30 40428.0 31 48676.0 32 58660.0 33 68995.5 34 78581.0 35 87178.0 36 97527.5 37 107763.0 38 110096.5 39 109569.5 40 109853.5 41 108252.0 42 107019.5 43 109278.0 44 111967.5 45 114332.5 46 113518.5 47 110139.0 48 109080.5 49 106628.0 50 100276.5 51 91249.0 52 81402.0 53 72053.0 54 62769.0 55 51947.5 56 40868.0 57 31352.5 58 23789.0 59 17950.5 60 13189.5 61 9187.5 62 6071.5 63 3728.0 64 2182.0 65 1281.5 66 716.0 67 385.5 68 235.0 69 152.0 70 101.5 71 62.0 72 45.5 73 41.0 74 31.5 75 25.5 76 16.0 77 7.5 78 6.5 79 5.0 80 3.0 81 4.0 82 3.5 83 0.5 84 0.0 85 0.0 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.004626541123234638 2 0.0070144333158718725 3 0.0026117570856969735 4 0.16875681855153443 5 0.0016789866979480545 6 5.223514171393947E-4 7 3.7310815509956765E-5 8 6.342838636692649E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 2.984865240796541E-4 24-25 5.783176404043298E-4 26-27 0.0029475544252865844 28-29 2.052094853047622E-4 30-31 0.006492081898732477 32-33 0.020297083637416478 34-35 0.013786346330929025 36-37 0.0010633582420337677 38-39 2.4252030081471898E-4 40-41 0.0018655407754978382 42-43 0.011696940662371445 44-45 0.00818972400443551 46-47 0.011025345983192224 48-49 0.011211900060742009 50-51 0.18517357737591542 52-53 0.11342487915026857 54-55 0.018133056337838988 56-57 0.007518129325256288 58-59 0.018394232046408682 60-61 0.12265930598898285 62-63 0.23625208380904625 64-65 0.24179273991227482 66-67 0.25209052499302287 68-69 0.2469789432681588 70-71 0.23365898213110423 72-73 0.1599141552756747 74-75 0.25779907976604627 76-77 0.20095605233662714 78-79 0.006156284559142865 80-81 3.7310815509956765E-5 82-83 0.0 84-85 0.0 86-87 0.0 88-89 9.327703877489192E-5 90-91 0.006025696704858017 92-93 0.017610704920699594 94-95 0.0029848652407965413 96-97 1.8655407754978382E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2680188.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.66552656238057 #Duplication Level Percentage of deduplicated Percentage of total 1 70.03587450774961 35.4840446019194 2 16.590166834923416 16.81099076898275 3 5.833784160474533 8.867152390251523 4 2.691603609628777 5.454860567161849 5 1.401776671147774 3.5510876583281474 6 0.8462118777453642 2.572426222158582 7 0.5310268823788662 1.883332963015321 8 0.3728888622677957 1.51140884448359 9 0.27503146336716133 1.254115252144738 >10 1.2534057383103525 11.504741834046888 >50 0.10150563461798547 3.592932746169633 >100 0.06362501030125206 5.751706474204771 >500 0.002213390776481413 0.723618876158379 >1k 7.377969254816768E-4 0.6109903156654978 >5k 1.4755938509633537E-4 0.4265904853089422 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6066 0.22632740688339772 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5346 0.1994636197162289 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3023 0.1127905952865993 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 3.7310815509956765E-5 0.0 0.0 0.0 0.0 8 3.7310815509956765E-5 0.0 0.0 0.0 0.0 9 3.7310815509956765E-5 0.0 0.0 0.0 0.0 10-11 3.7310815509956765E-5 0.0 0.0 0.0 0.0 12-13 3.7310815509956765E-5 0.0 0.0 0.0 0.0 14-15 3.7310815509956765E-5 0.0 0.0 0.0 1.8655407754978382E-5 16-17 3.7310815509956765E-5 0.0 0.0 3.7310815509956765E-5 3.7310815509956765E-5 18-19 5.5966223264935144E-5 0.0 0.0 3.7310815509956765E-5 3.7310815509956765E-5 20-21 7.462163101991353E-5 0.0 0.0 3.7310815509956765E-5 3.7310815509956765E-5 22-23 7.462163101991353E-5 0.0 0.0 1.1193244652987029E-4 3.7310815509956765E-5 24-25 7.462163101991353E-5 0.0 0.0 2.052094853047622E-4 3.7310815509956765E-5 26-27 7.462163101991353E-5 0.0 0.0 3.3579733958961086E-4 3.7310815509956765E-5 28-29 7.462163101991353E-5 0.0 0.0 7.462163101991353E-4 3.7310815509956765E-5 30-31 7.462163101991353E-5 0.0 0.0 0.002966209833041563 3.7310815509956765E-5 32-33 7.462163101991353E-5 0.0 0.0 0.009532913362793953 3.7310815509956765E-5 34-35 9.327703877489192E-5 0.0 0.0 0.018263644192123837 3.7310815509956765E-5 36-37 1.1193244652987029E-4 0.0 0.0 0.034288639453650266 3.7310815509956765E-5 38-39 1.1193244652987029E-4 0.0 0.0 0.058969743913486666 3.7310815509956765E-5 40-41 1.1193244652987029E-4 0.0 0.0 0.08896763958349191 3.7310815509956765E-5 42-43 1.1193244652987029E-4 0.0 0.0 0.11965578534043134 3.7310815509956765E-5 44-45 1.1193244652987029E-4 0.0 0.0 0.15623903994794397 3.7310815509956765E-5 46-47 1.1193244652987029E-4 0.0 0.0 0.20114260641417692 3.7310815509956765E-5 48-49 1.1193244652987029E-4 0.0 0.0 0.25317253864281164 3.7310815509956765E-5 50-51 1.1193244652987029E-4 0.0 0.0 0.3076090184718385 3.7310815509956765E-5 52-53 1.1193244652987029E-4 0.0 0.0 0.36441473508574773 3.7310815509956765E-5 54-55 1.1193244652987029E-4 0.0 0.0 0.425436573852282 3.7310815509956765E-5 56-57 1.1193244652987029E-4 0.0 0.0 0.498752326329347 3.7310815509956765E-5 58-59 1.8655407754978383E-4 0.0 0.0 0.5818808232855307 3.7310815509956765E-5 60-61 1.8655407754978383E-4 0.0 0.0 0.6753257607302174 3.7310815509956765E-5 62-63 1.8655407754978383E-4 0.0 0.0 0.7724271580948798 3.7310815509956765E-5 64-65 1.8655407754978383E-4 0.0 0.0 0.8740245087284921 3.7310815509956765E-5 66-67 1.8655407754978383E-4 0.0 0.0 0.9910498815754716 3.7310815509956765E-5 68-69 1.8655407754978383E-4 0.0 0.0 1.1274209122643635 3.7310815509956765E-5 70-71 1.8655407754978383E-4 0.0 0.0 1.2811414721653853 3.7310815509956765E-5 72-73 1.8655407754978383E-4 0.0 0.0 1.4460552767193944 3.7310815509956765E-5 74-75 1.8655407754978383E-4 0.0 0.0 1.6109131150501383 3.7310815509956765E-5 76-77 1.8655407754978383E-4 0.0 0.0 1.795004678776265 3.7310815509956765E-5 78-79 2.052094853047622E-4 0.0 0.0 2.0051391917283414 3.7310815509956765E-5 80-81 2.4252030081471898E-4 0.0 0.0 2.2444507624092043 3.7310815509956765E-5 82-83 2.611757085696974E-4 0.0 0.0 2.499190355303434 3.7310815509956765E-5 84-85 2.611757085696974E-4 0.0 0.0 2.7472513122213815 3.7310815509956765E-5 86-87 2.611757085696974E-4 0.0 0.0 3.016989852950614 3.7310815509956765E-5 88-89 2.611757085696974E-4 0.0 0.0 3.323237026656339 3.7310815509956765E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3615 0.0 58.367867 1 GTATCAA 6805 0.0 40.85327 1 CAACGCA 8520 0.0 31.906282 5 ATCAACG 8525 0.0 31.829117 3 TCAACGC 8675 0.0 31.333538 4 TATCAAC 8980 0.0 30.482697 2 AACGCAG 9075 0.0 30.11126 6 ACGCAGA 10490 0.0 25.778193 7 CGCAGAG 10530 0.0 25.635216 8 GTACATG 8730 0.0 23.2441 1 TACATGG 8495 0.0 22.93653 2 ACATGGG 8170 0.0 22.451645 3 GCAGAGT 11980 0.0 21.977644 9 GTGGTAT 1700 0.0 21.245342 1 GTATAGG 855 0.0 20.565285 1 AGAGTAC 11015 0.0 20.371433 10-11 GAGTACT 7805 0.0 20.24027 12-13 CAGAGTA 11705 0.0 19.677176 10-11 GAGTACA 6345 0.0 19.473356 1 AGTACAT 6440 0.0 18.300924 2 >>END_MODULE