FastQCFastQC Report
Sat 18 Jun 2016
SRR1931011_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931011_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2765987
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1234084.461626175394172No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20600.054.5061841
GTATCAA40650.046.0363851
TCAACGC48150.038.5183224
ATCAACG48250.037.8169063
CAACGCA50400.036.893745
TATCAAC50500.036.6468622
AACGCAG53850.033.996716
ACGCAGA60900.028.7538557
CGCAGAG62150.027.925938
GTACATG45000.026.7746771
TACATGG46600.024.8624232
ACATGGG45650.024.843713
GCAGAGT68350.024.0785089
GTGGTAT10650.021.6636561
AGAGTAC59900.020.46825610-11
CATGGGG30850.020.0939714
CAGAGTA64850.019.7186910-11
GAGTACT46600.019.69249212-13
AGTACTT51250.018.28074812-13
GTACTTT51800.017.97559414-15
Produced by FastQC (version 0.11.5)