FastQCFastQC Report
Sat 18 Jun 2016
SRR1931011_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931011_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2765987
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66960.24208356727634656No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54110.19562637134592464No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33770.12209023397434624No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35200.058.7488981
GTATCAA68250.042.071441
CAACGCA84300.033.8229835
ATCAACG84200.033.702973
TCAACGC85050.033.475494
TATCAAC87700.032.4686782
AACGCAG88700.032.3579566
CGCAGAG102200.027.711318
ACGCAGA102950.027.509437
GCAGAGT119750.023.1350779
GTACATG72050.022.1696281
ACATGGG68400.021.2652853
GAGTACT84200.020.81785412-13
AGAGTAC110600.020.80272510-11
GTGGTAT17950.020.6577631
TACATGG74150.020.5793322
CAGAGTA116850.020.1569210-11
GACCGTG5500.019.838047
CCGCGAT1200.00480083819.7661639
GCCGCGA1250.006078338318.9755158
Produced by FastQC (version 0.11.5)