##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931011_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2765987 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.337994357891052 31.0 31.0 34.0 30.0 34.0 2 31.49667442399404 31.0 31.0 34.0 30.0 34.0 3 31.56763137353863 31.0 31.0 34.0 30.0 34.0 4 35.33868018902475 37.0 35.0 37.0 33.0 37.0 5 35.211181758988744 37.0 35.0 37.0 33.0 37.0 6 35.23494217434862 37.0 35.0 37.0 32.0 37.0 7 35.12016614684017 37.0 35.0 37.0 32.0 37.0 8 35.12244273020806 37.0 35.0 37.0 32.0 37.0 9 36.62831748666932 39.0 35.0 39.0 32.0 39.0 10-11 36.568972124597835 38.5 35.0 39.0 32.0 39.0 12-13 36.45050121349088 38.0 35.0 39.0 32.0 39.0 14-15 37.51395125862848 39.0 36.0 41.0 32.0 41.0 16-17 37.47405211955081 39.0 36.0 41.0 32.0 41.0 18-19 37.494935261807086 39.0 36.0 41.0 32.0 41.0 20-21 37.48270960781811 39.0 36.0 41.0 32.0 41.0 22-23 37.377533227741125 39.0 36.0 41.0 32.0 41.0 24-25 37.25424034892427 39.0 36.0 40.5 31.0 41.0 26-27 37.004278581208084 39.0 36.0 40.0 31.0 41.0 28-29 36.81736519369035 39.0 36.0 40.0 30.5 41.0 30-31 36.58909640573148 38.5 35.0 40.0 30.0 41.0 32-33 36.43000635939359 38.0 35.0 40.0 30.0 41.0 34-35 36.506001835872695 38.0 35.0 40.0 30.0 41.0 36-37 36.57741901896141 38.5 35.0 40.0 30.0 41.0 38-39 36.56690920817777 39.0 35.0 40.0 30.0 41.0 40-41 36.45600702389419 39.0 35.0 40.0 30.0 41.0 42-43 36.321384012289286 39.0 35.0 40.0 30.0 41.0 44-45 36.1525383163406 38.0 35.0 40.0 30.0 41.0 46-47 35.908455281966255 38.0 35.0 40.0 29.0 41.0 48-49 35.69043202299939 38.0 35.0 40.0 28.0 41.0 50-51 35.433649182009894 38.0 34.5 40.0 27.5 41.0 52-53 35.23108785399208 38.0 34.0 40.0 27.0 41.0 54-55 35.08293603693727 38.0 34.0 40.0 26.0 41.0 56-57 34.91036743845868 38.0 34.0 40.0 26.0 41.0 58-59 34.70827285160776 38.0 33.5 40.0 26.0 41.0 60-61 34.40605848834431 37.5 33.0 40.0 26.0 41.0 62-63 34.150448104058334 37.0 33.0 40.0 25.0 41.0 64-65 33.845483366335415 37.0 32.5 39.5 24.5 41.0 66-67 33.52921506861746 36.0 32.0 39.0 23.5 41.0 68-69 33.1823461209326 36.0 32.0 39.0 23.0 40.0 70-71 32.80441647050402 35.5 31.0 39.0 22.0 40.0 72-73 32.37633004059672 35.0 31.0 38.0 22.0 40.0 74-75 31.865508044687125 35.0 31.0 37.0 21.0 39.5 76-77 30.476312976163662 33.5 29.5 36.0 20.0 38.5 78-79 31.212142175650136 34.0 30.0 36.0 20.0 39.0 80-81 31.176479137465215 34.0 31.0 36.0 20.0 38.5 82-83 30.905455629401004 34.0 30.0 36.0 20.0 37.0 84-85 30.5632020324029 34.0 30.0 35.0 19.0 37.0 86-87 30.21472317115012 34.0 30.0 35.0 18.0 36.5 88-89 29.863323471874594 34.0 30.0 35.0 15.5 36.0 90-91 29.524459261739118 34.0 29.0 35.0 9.0 36.0 92-93 29.20566257180529 34.0 29.0 35.0 4.5 35.0 94-95 28.82129145943202 34.0 29.0 35.0 2.0 35.0 96-97 28.393086265409057 34.0 29.0 35.0 2.0 35.0 98-99 27.902324920543734 33.5 29.0 35.0 2.0 35.0 100-101 26.766549336638242 32.5 26.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3243106423725237 1101 2 0.32771242603818607 1101 3 0.3148386100605869 1101 4 0.21305429176997848 1101 5 0.24929703235407885 1101 6 0.2876222431396229 1101 7 0.4535413860917856 1101 8 0.45117777053707186 1101 9 0.501002277531569 1101 10-11 0.4652805733662646 1101 12-13 0.4425238556785871 1101 14-15 0.5751028436719992 1101 16-17 0.6309870368670332 1101 18-19 0.46075419507644 1101 20-21 0.48602618766287975 1101 22-23 0.5278493790627365 1101 24-25 0.47965474506786876 1101 26-27 0.4286178677045811 1101 28-29 0.5298162231783934 1101 30-31 0.535365811922901 1101 32-33 0.5384302121833642 1101 34-35 0.5202423361491668 1101 36-37 0.5449695286673943 1101 38-39 0.582781224327789 1101 40-41 0.6215967948603733 1101 42-43 0.5475445371246792 1101 44-45 0.4808575506785786 1101 46-47 0.5354679891361158 1101 48-49 0.5558476360763223 1101 50-51 0.6367979805205835 1101 52-53 0.4995958670526832 1101 54-55 0.5004929978645691 1101 56-57 0.5676789542819449 1101 58-59 0.527699985908697 1101 60-61 0.47900422599511217 1101 62-63 0.30082530560109433 1101 64-65 0.4065706844431034 1101 66-67 0.37314379823131816 1101 68-69 0.40320734254332535 1101 70-71 0.2370482841442012 1101 72-73 0.2873837467289917 1101 74-75 0.19721830331103973 1101 76-77 0.1533322413051259 1101 78-79 0.4992547652027639 1101 80-81 0.26390164995614995 1101 82-83 0.3854774599667188 1101 84-85 0.5203122119057859 1101 86-87 0.3883855895631356 1101 88-89 0.4280959689333912 1101 90-91 0.20359329628898948 1101 92-93 0.15664665705415004 1101 94-95 0.4017440440511315 1101 96-97 0.4118320740855381 1101 98-99 0.3711063223214133 1101 100-101 0.6681361035346356 1102 1 0.3343238692819881 1102 2 0.4578003596558524 1102 3 0.4498250646419244 1102 4 0.19984995382520054 1102 5 0.3217365569878865 1102 6 0.32730171755430604 1102 7 0.4113261270434947 1102 8 0.39649375423140754 1102 9 0.477868478088034 1102 10-11 0.4925748051396397 1102 12-13 0.5461424211031343 1102 14-15 0.6172019848006656 1102 16-17 0.6118027514587183 1102 18-19 0.6737863123399919 1102 20-21 0.5860983914405509 1102 22-23 0.6653731230455477 1102 24-25 0.721178638524286 1102 26-27 0.6406915732405807 1102 28-29 0.6126572048287571 1102 30-31 0.6663962318083065 1102 32-33 0.7753936558546357 1102 34-35 0.6861534003561118 1102 36-37 0.6756819409457009 1102 38-39 0.5676139167136114 1102 40-41 0.7385508638279035 1102 42-43 0.7783579945645798 1102 44-45 0.7725119075081963 1102 46-47 0.7758623035212437 1102 48-49 0.6967156012238931 1102 50-51 0.7049442770611734 1102 52-53 0.5718390194811462 1102 54-55 0.6666853282634406 1102 56-57 0.6112631758881051 1102 58-59 0.6507379567192118 1102 60-61 0.5549585121507974 1102 62-63 0.23737739589920892 1102 64-65 0.36342898998420736 1102 66-67 0.2519544347330722 1102 68-69 0.38241069837628316 1102 70-71 0.4673098679725598 1102 72-73 0.34172756010165983 1102 74-75 0.3584915475855155 1102 76-77 0.47090735304667675 1102 78-79 0.5282436163777113 1102 80-81 0.519295490339406 1102 82-83 0.39462807870217986 1102 84-85 0.578089780130707 1102 86-87 0.4618050942637808 1102 88-89 0.5735220013169986 1102 90-91 0.5148407337947241 1102 92-93 0.539553773251825 1102 94-95 0.6838566678997964 1102 96-97 0.8920393608737243 1102 98-99 1.0815418372504695 1102 100-101 1.109977829041906 1103 1 0.33074534943082057 1103 2 0.4168184063493534 1103 3 0.288331106215967 1103 4 0.2375907751573365 1103 5 0.3163214202218825 1103 6 0.3617513508198158 1103 7 0.2877803301357815 1103 8 0.24645184452278812 1103 9 0.4537735854779825 1103 10-11 0.3679164870439635 1103 12-13 0.5036195253483768 1103 14-15 0.43561381786457787 1103 16-17 0.4348345900778767 1103 18-19 0.46958837288431 1103 20-21 0.4756639781715606 1103 22-23 0.379354820182634 1103 24-25 0.455794487460885 1103 26-27 0.4258888580410911 1103 28-29 0.44319868415313834 1103 30-31 0.4666001613096995 1103 32-33 0.5160656785231694 1103 34-35 0.4053654683892134 1103 36-37 0.45534115710655954 1103 38-39 0.527491627717211 1103 40-41 0.4659886936705604 1103 42-43 0.4878945144098523 1103 44-45 0.6114040434998032 1103 46-47 0.5642863659755406 1103 48-49 0.525847044596226 1103 50-51 0.4284128305480692 1103 52-53 0.5165989318142792 1103 54-55 0.5616443362498416 1103 56-57 0.5048662526692382 1103 58-59 0.625336575563658 1103 60-61 0.43263479547613315 1103 62-63 0.3010889864364472 1103 64-65 0.2699507099115408 1103 66-67 0.25059371875428127 1103 68-69 0.08395222971973482 1103 70-71 0.3257765124575016 1103 72-73 0.27859517253402544 1103 74-75 0.42163902559273936 1103 76-77 0.4110709383191953 1103 78-79 0.4110782536231774 1103 80-81 0.2509078510534195 1103 82-83 0.2012547691994726 1103 84-85 0.20560043668015382 1103 86-87 0.2595870695557032 1103 88-89 0.28489366583910325 1103 90-91 0.24689721964111655 1103 92-93 0.154678246067931 1103 94-95 0.23177826042946492 1103 96-97 0.25766390386650784 1103 98-99 0.3366221625561785 1103 100-101 0.507709551943158 1104 1 0.3614311276697002 1104 2 0.3945483323580419 1104 3 0.3518702433260117 1104 4 0.1406724164191786 1104 5 0.27408043731930576 1104 6 0.29176600087168936 1104 7 0.34586607569231376 1104 8 0.25577248874504477 1104 9 0.3962583558844983 1104 10-11 0.442783287480232 1104 12-13 0.4064857386424947 1104 14-15 0.5143053554169725 1104 16-17 0.5386105411911686 1104 18-19 0.5963113215920259 1104 20-21 0.5042073525858441 1104 22-23 0.544496389847339 1104 24-25 0.472430419420796 1104 26-27 0.5108040549522315 1104 28-29 0.5583360260142811 1104 30-31 0.6206717736514662 1104 32-33 0.6314281865512541 1104 34-35 0.4894417550665864 1104 36-37 0.5212317130484792 1104 38-39 0.5796050069578484 1104 40-41 0.42478566029276266 1104 42-43 0.5294108327815152 1104 44-45 0.5634966162465247 1104 46-47 0.4985988871145537 1104 48-49 0.4731016324756254 1104 50-51 0.38540086728910694 1104 52-53 0.30366560570146817 1104 54-55 0.42737214550653846 1104 56-57 0.5589712124053463 1104 58-59 0.677474894094928 1104 60-61 0.5530001252068075 1104 62-63 0.30282666120149315 1104 64-65 0.25940210252152696 1104 66-67 0.38344104469228313 1104 68-69 0.31019031111398476 1104 70-71 0.3049453167776548 1104 72-73 0.3729580852911596 1104 74-75 0.40890098600149827 1104 76-77 0.5445892499339671 1104 78-79 0.6076053267670254 1104 80-81 0.6077444469210072 1104 82-83 0.5815661255548612 1104 84-85 0.48029650985820993 1104 86-87 0.42014242243003963 1104 88-89 0.6128626765140694 1104 90-91 0.5667241457144101 1104 92-93 0.4693724286102352 1104 94-95 0.639778879873937 1104 96-97 0.7158112613467793 1104 98-99 0.6663067589101992 1104 100-101 0.8915394859324195 1105 1 0.3495299517149597 1105 2 0.23654634080693526 1105 3 0.2886571491998886 1105 4 0.19261947542694458 1105 5 0.2299964126542875 1105 6 0.23320200870524843 1105 7 0.23875364977672575 1105 8 0.2259634731534632 1105 9 0.3810504405281847 1105 10-11 0.3767698624729192 1105 12-13 0.4859424790573499 1105 14-15 0.5181589192986067 1105 16-17 0.5225118626894201 1105 18-19 0.48921248280817764 1105 20-21 0.5563589094408528 1105 22-23 0.5062887996610286 1105 24-25 0.5850793712311599 1105 26-27 0.5126127555065878 1105 28-29 0.43217121733030694 1105 30-31 0.4389328326555457 1105 32-33 0.5456086383993792 1105 34-35 0.5463009076348797 1105 36-37 0.6031701776196812 1105 38-39 0.6129251446530617 1105 40-41 0.5729742883701334 1105 42-43 0.38682896916130716 1105 44-45 0.47210553806386457 1105 46-47 0.47137154169926987 1105 48-49 0.5389697442604202 1105 50-51 0.4962573540160946 1105 52-53 0.44079004816848766 1105 54-55 0.6890252987343857 1105 56-57 0.7386624114443947 1105 58-59 0.7278205138800047 1105 60-61 0.7752914729213103 1105 62-63 0.6038032765963166 1105 64-65 0.5450602631831458 1105 66-67 0.7489808314986774 1105 68-69 0.639216869120439 1105 70-71 0.5972514997977001 1105 72-73 0.7002157829770113 1105 74-75 0.7844343384949894 1105 76-77 0.7173335097162408 1105 78-79 0.8243310168551439 1105 80-81 0.7111825900068602 1105 82-83 0.8249346841029599 1105 84-85 0.8571669282313259 1105 86-87 0.7908539551874618 1105 88-89 0.8817970354685905 1105 90-91 0.8917639168610556 1105 92-93 0.8752604697235142 1105 94-95 0.8675818821234174 1105 96-97 0.8228254684201914 1105 98-99 0.8951914350206103 1105 100-101 1.057988067620098 1106 1 0.20232064535758099 1106 2 0.1824748094534563 1106 3 0.20622653946704617 1106 4 0.15987593769354902 1106 5 0.14013181252599338 1106 6 0.1377886143466185 1106 7 0.14071027327493368 1106 8 0.17853745765442142 1106 9 0.3005292983843191 1106 10-11 0.2641111013557378 1106 12-13 0.23357410818999114 1106 14-15 0.208168801235459 1106 16-17 0.2668361635857295 1106 18-19 0.22448198229674432 1106 20-21 0.25176900982052786 1106 22-23 0.2021063132270129 1106 24-25 0.25393202635157053 1106 26-27 0.33869292055226907 1106 28-29 0.34683098397790246 1106 30-31 0.2523100279191439 1106 32-33 0.20371095978770626 1106 34-35 0.3346230587765078 1106 36-37 0.28321906720204026 1106 38-39 0.34156039716255293 1106 40-41 0.23190669245466466 1106 42-43 0.21270265373682662 1106 44-45 0.29278461783016496 1106 46-47 0.4626225602322407 1106 48-49 0.5240695171492362 1106 50-51 0.3726806129748468 1106 52-53 0.39176575940788183 1106 54-55 0.38908667076330516 1106 56-57 0.47896667107928437 1106 58-59 0.48227449352668117 1106 60-61 0.4553868249436448 1106 62-63 0.2174781435369013 1106 64-65 0.28128640350664824 1106 66-67 0.2604894738592449 1106 68-69 0.20971842075790192 1106 70-71 0.32680143825231056 1106 72-73 0.42867974858094726 1106 74-75 0.5384176258859839 1106 76-77 0.48339651139790263 1106 78-79 0.439131037659223 1106 80-81 0.35757863029742154 1106 82-83 0.3925260920189153 1106 84-85 0.45229946754236394 1106 86-87 0.38978947988363544 1106 88-89 0.46995535449911685 1106 90-91 0.4724561725431684 1106 92-93 0.42601912508663276 1106 94-95 0.6901478917279675 1106 96-97 0.528794505018034 1106 98-99 0.5615134474683856 1106 100-101 0.8555813825764957 1107 1 0.16524931943669685 1107 2 0.1989183462509736 1107 3 0.16064602362104452 1107 4 0.105294589837861 1107 5 0.1689253984877226 1107 6 0.1933925777381944 1107 7 0.2141797536470662 1107 8 0.21467541998707418 1107 9 0.27100354390281467 1107 10-11 0.1929606588778583 1107 12-13 0.19846027413810674 1107 14-15 0.23656226617760012 1107 16-17 0.2615476075007095 1107 18-19 0.35085063822292284 1107 20-21 0.38067756439280487 1107 22-23 0.31872381568522457 1107 24-25 0.20356216022948814 1107 26-27 0.25109894127687227 1107 28-29 0.21466700243339432 1107 30-31 0.46006208903403945 1107 32-33 0.3682976022504576 1107 34-35 0.3037925618398063 1107 36-37 0.29943191040603523 1107 38-39 0.25583995241556323 1107 40-41 0.28125394304085916 1107 42-43 0.19851915778341578 1107 44-45 0.19219162183894412 1107 46-47 0.18996164199609922 1107 48-49 0.30945715802360496 1107 50-51 0.1081378401134998 1107 52-53 0.27251002988666784 1107 54-55 0.2103868749838611 1107 56-57 0.2385468843763121 1107 58-59 0.1348491853049154 1107 60-61 0.11821263237122537 1107 62-63 0.0368692637244763 1107 64-65 0.10362132522210032 1107 66-67 0.14967864260593444 1107 68-69 0.08608178207700945 1107 70-71 0.1985229419207073 1107 72-73 0.1840704149450616 1107 74-75 0.21811961514549694 1107 76-77 0.3223285135157461 1107 78-79 0.471089285900657 1107 80-81 0.341746242429636 1107 82-83 0.4667194208140124 1107 84-85 0.3671175599710743 1107 86-87 0.4018574021285488 1107 88-89 0.457345869588746 1107 90-91 0.5164725101878211 1107 92-93 0.36307305444144333 1107 94-95 0.5132036363766304 1107 96-97 0.5539060699526033 1107 98-99 0.59514025330758 1107 100-101 0.6491159109782814 1108 1 0.14323618932397153 1108 2 0.19850552073840433 1108 3 0.08281353063031105 1108 4 0.045899405750908784 1108 5 -7.055045825055117E-4 1108 6 0.15781758725695028 1108 7 0.16103187257957785 1108 8 0.20994119515985687 1108 9 0.13727853784542532 1108 10-11 0.12656220956955622 1108 12-13 0.10911227999934425 1108 14-15 0.30665641531629007 1108 16-17 0.20909315330035128 1108 18-19 0.27287253712797366 1108 20-21 0.1721688359830651 1108 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-0.9683052479142873 2316 86-87 -0.8846298492053961 2316 88-89 -0.9527162184230242 2316 90-91 -0.8473475772378016 2316 92-93 -0.6901029861607668 2316 94-95 -1.1694132709767047 2316 96-97 -0.9715481540945596 2316 98-99 -0.9576999941140762 2316 100-101 -1.3712889326309465 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 16.0 4 25.0 5 63.0 6 119.0 7 366.0 8 964.0 9 1708.0 10 3031.0 11 5084.0 12 7234.0 13 9545.0 14 11545.0 15 14248.0 16 17373.0 17 20504.0 18 17394.0 19 13692.0 20 13092.0 21 13849.0 22 15565.0 23 18177.0 24 22375.0 25 28860.0 26 36878.0 27 47777.0 28 60357.0 29 76589.0 30 93443.0 31 113020.0 32 136223.0 33 166462.0 34 205791.0 35 257135.0 36 338257.0 37 466760.0 38 450665.0 39 81783.0 40 14.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.07643937564266 21.71728639131812 15.042561930284048 23.163712302755172 2 14.769052549981488 22.023057143187707 40.90415731442059 22.303732992410218 3 18.04963178736692 26.91876761840235 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28.00287782268852 30.351069060513815 20.924084772847237 24-25 20.68697418988637 28.16729754841882 30.473450543570614 20.672277718124203 26-27 20.65602516359955 28.350211504392785 30.290592573845764 20.7031707581619 28-29 20.63678627092068 28.4335080873599 30.33051902343579 20.59918661828363 30-31 20.97678006458549 28.207766203634655 30.29366096468226 20.52179276709759 32-33 20.799702901680742 28.36173383773029 30.106372842118244 20.732190418470722 34-35 20.8920466194128 28.354887803100127 30.220069286227474 20.5329962912596 36-37 20.912374320762698 28.212977725716478 30.299605563328598 20.57504239019223 38-39 20.79247457005768 28.38363163311107 30.296352307490125 20.527541489341125 40-41 20.94705804834479 28.33471532057133 30.127484481340822 20.590742149743065 42-43 20.84788526754286 28.348620171105075 29.9868675535648 20.816627007787268 44-45 20.84624860301766 28.550667207080238 29.875861555299167 20.72722263460293 46-47 20.93872781958856 28.511366288576802 29.590351522155057 20.95955436967958 48-49 20.966821967888425 28.644894846712432 29.478571522838426 20.909711662560714 50-51 21.067489340047285 28.79653231217521 29.217877752594923 20.91810059518258 52-53 21.112863749982807 28.814420638637795 29.112576362250426 20.96013924912897 54-55 21.01994594466569 28.76109722673969 29.237991183871127 20.980965644723494 56-57 21.036618380037243 28.81827948805603 29.17014741248542 20.974954719421305 58-59 20.98229661775849 28.77001658115736 29.329230622365632 20.918456178718518 60-61 21.10936594473731 28.9149231996079 29.228835956331668 20.746874899323124 62-63 21.044718838403202 28.815249400769883 29.27437527497179 20.865656485855126 64-65 20.856395339565996 28.631033752919294 29.334527628742535 21.17804327877218 66-67 20.890264858679576 28.789884386882946 29.15698149617444 21.162869258263036 68-69 20.937119325515035 28.778800782723824 29.175526796403005 21.108553095358133 70-71 20.976701533160554 28.602314861707878 29.23769959054957 21.183284014581993 72-73 21.044237103516604 28.728859529941396 29.096717469458937 21.130185897083066 74-75 20.928642354910846 28.914963707436137 28.949832695911066 21.206561241741948 76-77 20.887002442663697 28.87267973212206 28.97445436732547 21.265863457888777 78-79 20.871405847685114 28.86208278565255 29.02590344054107 21.24060792612126 80-81 20.86556192399557 28.900859314598225 28.949323088875982 21.28425567253022 82-83 20.988854972926482 28.876093777736482 28.998437085929908 21.13661416340713 84-85 21.07256830925091 28.90111197196516 28.80497992217606 21.221339796607865 86-87 20.852682966333536 28.92990820275005 28.9347166129125 21.282692218003916 88-89 20.925580525982987 28.856739177355756 28.855925723332355 21.361754573328902 90-91 20.978652215948 28.792409757127558 28.991772404073444 21.237165622850995 92-93 20.91491084683439 28.83805367830371 28.89028667035377 21.356748804508126 94-95 20.73966748677196 28.887326209685437 28.86839931234081 21.504606991201793 96-97 20.80500783536284 28.722240228663527 28.950983293296368 21.521768642677266 98-99 20.67797137152127 28.83574651652376 28.828208520141274 21.6580735918137 100-101 20.75611707502602 28.75640774884336 28.785908972095676 21.701566204034943 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 186.0 1 136.0 2 125.5 3 188.5 4 170.0 5 159.5 6 305.0 7 610.5 8 1022.0 9 1314.0 10 1323.0 11 1273.0 12 1378.5 13 1598.5 14 1897.0 15 2250.5 16 2568.0 17 2970.0 18 3456.0 19 3809.5 20 4333.0 21 5132.5 22 6284.0 23 8040.5 24 10340.5 25 13780.0 26 18515.5 27 23836.5 28 29535.0 29 36750.5 30 44700.0 31 52492.5 32 61571.5 33 69752.0 34 76925.0 35 83634.5 36 92569.5 37 101821.0 38 106304.0 39 108738.0 40 110780.0 41 112079.0 42 112762.5 43 114449.0 44 116284.0 45 118137.5 46 117569.5 47 114978.0 48 113156.5 49 109455.0 50 101696.0 51 92523.5 52 84234.5 53 75526.5 54 66487.5 55 55791.5 56 44482.5 57 34512.0 58 26481.0 59 20248.0 60 14779.5 61 10325.5 62 6994.5 63 4464.5 64 2635.0 65 1509.0 66 801.5 67 428.0 68 237.5 69 134.5 70 91.5 71 71.0 72 52.5 73 35.5 74 26.0 75 17.0 76 12.5 77 10.0 78 6.0 79 6.5 80 3.0 81 2.0 82 2.0 83 0.0 84 1.0 85 1.0 86 0.5 87 0.5 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0048807170821844065 2 0.005893013958489321 3 0.0020969006723458934 4 0.17252431049025177 5 0.0017715195335335993 6 4.699949782844244E-4 7 0.0 8 6.869157374926202E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 2.530742190762285E-4 24-25 6.146088177565548E-4 26-27 0.0031453510085188395 28-29 1.4461383947213057E-4 30-31 0.0070680014042003815 32-33 0.020697855774448687 34-35 0.012942938632755686 36-37 6.146088177565548E-4 38-39 1.9884402927417954E-4 40-41 0.0015365220443913873 42-43 0.01149680023803438 44-45 0.007375305813078659 46-47 0.01035796625219135 48-49 0.01035796625219135 50-51 0.18327996480099149 52-53 0.11457031432179543 54-55 0.01762481168566591 56-57 0.006381085666707761 58-59 0.01802249974421427 60-61 0.12422328810656015 62-63 0.23908283010729983 64-65 0.2443612352480326 66-67 0.25466497131042193 68-69 0.24960348692889736 70-71 0.23704015962475602 72-73 0.16120827755155756 74-75 0.2562738002745494 76-77 0.19797634623734675 78-79 0.005694169929215141 80-81 1.2653710953811425E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 1.4461383947213057E-4 90-91 0.006254548557169646 92-93 0.01784173244487411 94-95 0.0029645837091786765 96-97 5.423018980204896E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2765987.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.61541343778271 #Duplication Level Percentage of deduplicated Percentage of total 1 74.92475637894434 44.66670328255915 2 15.063196055900452 17.95997321133767 3 4.611139122744911 8.246848956647176 4 1.9713817579466835 4.700989541747777 5 1.0108909554176775 3.013234112387001 6 0.5795481992495037 2.073000330322898 7 0.3755666413907081 1.5672692419962582 8 0.25722481385078416 1.2267650899336955 9 0.1987128856809075 1.0661715751753937 >10 0.9238315222113282 9.699438848270674 >50 0.05837679332760163 2.384708036383789 >100 0.024524878744208906 2.529732462255159 >500 6.071389935418293E-4 0.2578520552139907 >1k 1.2142779869542585E-4 0.16910230233950066 >5k 1.2142779869542585E-4 0.4382109534298661 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6696 0.24208356727634656 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5411 0.19562637134592464 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3377 0.12209023397434624 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 1.807672993401632E-4 0.0 0.0 0.0 0.0 7 1.807672993401632E-4 0.0 0.0 0.0 0.0 8 1.807672993401632E-4 0.0 0.0 0.0 0.0 9 1.807672993401632E-4 0.0 0.0 0.0 3.615345986803264E-5 10-11 1.807672993401632E-4 0.0 0.0 0.0 3.615345986803264E-5 12-13 1.807672993401632E-4 0.0 0.0 0.0 9.03836496700816E-5 14-15 1.807672993401632E-4 0.0 0.0 0.0 1.4461383947213057E-4 16-17 2.8922767894426114E-4 0.0 0.0 0.0 1.4461383947213057E-4 18-19 2.8922767894426114E-4 0.0 0.0 0.0 1.6269056940614688E-4 20-21 2.8922767894426114E-4 0.0 0.0 0.0 1.807672993401632E-4 22-23 2.8922767894426114E-4 0.0 0.0 1.807672993401632E-5 1.807672993401632E-4 24-25 3.073044088782775E-4 0.0 0.0 1.0846037960409793E-4 1.807672993401632E-4 26-27 3.2538113881229377E-4 0.0 0.0 1.4461383947213057E-4 1.807672993401632E-4 28-29 3.2538113881229377E-4 0.0 0.0 4.3384151841639165E-4 1.807672993401632E-4 30-31 3.2538113881229377E-4 0.0 0.0 0.002042670482543844 1.807672993401632E-4 32-33 3.2538113881229377E-4 0.0 0.0 0.006869157374926202 1.807672993401632E-4 34-35 3.2538113881229377E-4 0.0 0.0 0.013629854370248306 2.1692075920819585E-4 36-37 3.2538113881229377E-4 0.0 0.0 0.025271268447754815 2.1692075920819585E-4 38-39 3.2538113881229377E-4 0.0 0.0 0.04354684241104531 2.1692075920819585E-4 40-41 3.2538113881229377E-4 0.0 0.0 0.06576314349995137 2.1692075920819585E-4 42-43 3.615345986803264E-4 0.0 0.0 0.09047403331975168 2.1692075920819585E-4 44-45 3.615345986803264E-4 0.0 0.0 0.11776989552011632 2.1692075920819585E-4 46-47 3.615345986803264E-4 0.0 0.0 0.15124799935791455 2.1692075920819585E-4 48-49 3.615345986803264E-4 0.0 0.0 0.1875099196055513 2.1692075920819585E-4 50-51 4.51918248350408E-4 0.0 0.0 0.2268448839419708 2.1692075920819585E-4 52-53 4.6999497828442433E-4 0.0 0.0 0.26796944454185795 2.1692075920819585E-4 54-55 4.6999497828442433E-4 0.0 0.0 0.31205858885092375 2.1692075920819585E-4 56-57 4.6999497828442433E-4 0.0 0.0 0.36321573456418993 2.1692075920819585E-4 58-59 4.6999497828442433E-4 0.0 0.0 0.424242774821429 2.1692075920819585E-4 60-61 5.242251680864733E-4 0.0 0.0 0.4876920968898263 2.1692075920819585E-4 62-63 5.784553578885223E-4 0.0 0.0 0.5574501977051953 2.1692075920819585E-4 64-65 5.784553578885223E-4 0.0 0.0 0.6311671023761138 2.1692075920819585E-4 66-67 5.784553578885223E-4 0.0 0.0 0.7115723971226184 2.1692075920819585E-4 68-69 5.784553578885223E-4 0.0 0.0 0.8081744418900016 2.1692075920819585E-4 70-71 5.784553578885223E-4 0.0 0.0 0.9145921510115558 2.1692075920819585E-4 72-73 6.507622776245875E-4 0.0 0.0 1.0260713445146343 2.1692075920819585E-4 74-75 6.507622776245875E-4 0.0 0.0 1.1399908965588053 2.1692075920819585E-4 76-77 6.507622776245875E-4 0.0 0.0 1.2670703079949401 2.1692075920819585E-4 78-79 6.507622776245875E-4 0.0 0.0 1.4118649147664106 2.1692075920819585E-4 80-81 6.507622776245875E-4 0.0 0.0 1.5754412439393244 2.3499748914221217E-4 82-83 6.869157374926202E-4 0.0 0.0 1.749068234955551 2.530742190762285E-4 84-85 6.869157374926202E-4 0.0 0.0 1.9254067354618805 2.530742190762285E-4 86-87 7.411459272946691E-4 0.0 0.0 2.117092379682189 2.530742190762285E-4 88-89 7.95376117096718E-4 0.0 0.0 2.3394361578705904 2.530742190762285E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3520 0.0 58.748898 1 GTATCAA 6825 0.0 42.07144 1 CAACGCA 8430 0.0 33.822983 5 ATCAACG 8420 0.0 33.70297 3 TCAACGC 8505 0.0 33.47549 4 TATCAAC 8770 0.0 32.468678 2 AACGCAG 8870 0.0 32.357956 6 CGCAGAG 10220 0.0 27.71131 8 ACGCAGA 10295 0.0 27.50943 7 GCAGAGT 11975 0.0 23.135077 9 GTACATG 7205 0.0 22.169628 1 ACATGGG 6840 0.0 21.265285 3 GAGTACT 8420 0.0 20.817854 12-13 AGAGTAC 11060 0.0 20.802725 10-11 GTGGTAT 1795 0.0 20.657763 1 TACATGG 7415 0.0 20.579332 2 CAGAGTA 11685 0.0 20.15692 10-11 GACCGTG 550 0.0 19.83804 7 CCGCGAT 120 0.004800838 19.766163 9 GCCGCGA 125 0.0060783383 18.975515 8 >>END_MODULE