FastQCFastQC Report
Sat 18 Jun 2016
SRR1931010_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931010_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2962778
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1338184.516639451217743No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27100.061.7417031
GTATCAA53800.047.1275631
ATCAACG64150.037.5178683
TATCAAC65550.036.7156752
TCAACGC65850.036.6987954
CAACGCA65750.036.057585
AACGCAG72800.032.6442346
CGCAGAG82650.028.0826848
ACGCAGA83250.027.8847987
GTACATG64050.027.4054741
ACATGGG63750.026.672653
TACATGG64650.026.6699452
GCAGAGT89250.025.090059
GTGTAGC10050.024.0028111
GAGTACT55450.022.12933712-13
GTGGTAT13600.021.133891
CAGAGTA88500.020.91665610-11
CATGGGG36850.020.8102934
AGAGTAC80650.020.25726310-11
GAGTACA45150.020.007151
Produced by FastQC (version 0.11.5)