##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931010_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2962778 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 20.597159827702246 28.0 2.0 31.0 2.0 33.0 2 19.33182945195354 27.0 2.0 31.0 2.0 33.0 3 19.94092233707689 27.0 2.0 31.0 2.0 33.0 4 21.4578132414916 30.0 2.0 35.0 2.0 37.0 5 20.821683568596768 28.0 2.0 35.0 2.0 37.0 6 21.763349464590327 29.0 2.0 35.0 2.0 37.0 7 21.92174101468284 29.0 2.0 35.0 2.0 37.0 8 22.318192250651247 30.0 2.0 35.0 2.0 37.0 9 23.528281902997794 32.0 2.0 37.0 2.0 39.0 10-11 23.828666542008886 32.0 2.0 37.0 2.0 39.0 12-13 23.71126776964052 32.0 2.0 37.0 2.0 39.0 14-15 23.635133479457455 32.0 2.0 38.0 2.0 40.0 16-17 22.52439737300601 30.0 2.0 37.0 2.0 40.0 18-19 21.545347980847705 27.0 2.0 37.0 2.0 39.5 20-21 21.222628391327326 27.0 2.0 37.0 2.0 40.0 22-23 20.85421435558115 27.0 2.0 37.0 2.0 39.0 24-25 20.669743227470974 26.0 2.0 37.0 2.0 39.0 26-27 20.388703608572765 25.0 2.0 36.0 2.0 39.0 28-29 20.176845008299644 24.5 2.0 36.0 2.0 39.0 30-31 20.128994815001327 25.0 2.0 36.0 2.0 39.0 32-33 19.967271088147676 24.5 2.0 36.0 2.0 39.0 34-35 19.62908155791625 24.0 2.0 35.5 2.0 39.0 36-37 19.183505311569075 22.0 2.0 35.0 2.0 39.0 38-39 19.029209242136943 21.0 2.0 35.0 2.0 38.0 40-41 18.875411185043227 20.0 2.0 35.0 2.0 38.0 42-43 18.7159613376365 20.0 2.0 35.0 2.0 38.0 44-45 18.31384801696246 18.0 2.0 34.5 2.0 38.0 46-47 17.96341102843345 15.0 2.0 34.0 2.0 38.0 48-49 17.68779959213954 14.5 2.0 34.0 2.0 38.0 50-51 15.60578247847122 7.0 2.0 31.5 2.0 37.5 52-53 17.420249509075603 14.5 2.0 33.5 2.0 38.0 54-55 18.12423526163621 15.5 2.0 35.0 2.0 38.5 56-57 18.08447038556382 14.5 2.0 35.0 2.0 39.0 58-59 17.807550042561406 12.5 2.0 34.5 2.0 39.0 60-61 17.526067258498614 9.0 2.0 34.0 2.0 38.0 62-63 17.09799232342079 8.0 2.0 34.0 2.0 38.0 64-65 16.74268254320776 7.5 2.0 34.0 2.0 38.0 66-67 16.38432140376363 3.5 2.0 33.0 2.0 38.0 68-69 15.945130043492966 2.0 2.0 32.5 2.0 37.5 70-71 15.602856677078066 2.0 2.0 32.0 2.0 37.0 72-73 15.19687722131054 2.0 2.0 31.5 2.0 36.0 74-75 14.751318694819524 2.0 2.0 31.0 2.0 36.0 76-77 14.341583135827253 2.0 2.0 31.0 2.0 35.0 78-79 13.95939790291409 2.0 2.0 30.0 2.0 35.0 80-81 13.530134724910202 2.0 2.0 30.0 2.0 34.0 82-83 13.063635378688515 2.0 2.0 29.0 2.0 34.0 84-85 12.658593218931692 2.0 2.0 29.0 2.0 34.0 86-87 12.177383523166434 2.0 2.0 28.5 2.0 34.0 88-89 11.756660134508897 2.0 2.0 27.5 2.0 34.0 90-91 11.274975377837961 2.0 2.0 26.0 2.0 34.0 92-93 10.737687062614885 2.0 2.0 25.5 2.0 33.0 94-95 10.179702292915636 2.0 2.0 24.5 2.0 32.5 96-97 9.56321027090116 2.0 2.0 20.0 2.0 32.0 98-99 8.916202462688734 2.0 2.0 9.5 2.0 32.0 100-101 8.049643949023517 2.0 2.0 2.0 2.0 31.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.788437420095615 1101 2 9.559239308555252 1101 3 8.871242014497913 1101 4 10.925939516309747 1101 5 11.700707217628317 1101 6 10.84192031053625 1101 7 10.508826995744545 1101 8 9.85073236979052 1101 9 9.944484255802163 1101 10-11 9.296412953045955 1101 12-13 9.338575846112942 1101 14-15 9.768633663830833 1101 16-17 10.684897716350452 1101 18-19 11.699839506130349 1101 20-21 11.629249736387465 1101 22-23 11.966526393477668 1101 24-25 11.942794552377183 1101 26-27 11.896017289937195 1101 28-29 11.709999799615922 1101 30-31 11.525475674357931 1101 32-33 11.54736108608703 1101 34-35 11.370717431830244 1101 36-37 11.442896284444657 1101 38-39 11.318697471826873 1101 40-41 10.95160424100515 1101 42-43 10.41618320908049 1101 44-45 10.381307462498267 1101 46-47 10.308467789209107 1101 48-49 10.326202210049033 1101 50-51 11.943930639933042 1101 52-53 11.129897437304194 1101 54-55 10.851301205175002 1101 56-57 10.923269635564136 1101 58-59 10.903538241007627 1101 60-61 10.669452857814015 1101 62-63 10.785128028423518 1101 64-65 10.607484363336663 1101 66-67 9.99894048024461 1101 68-69 10.194639038095607 1101 70-71 9.92797839466708 1101 72-73 9.592947966238647 1101 74-75 9.335025395500928 1101 76-77 9.132005669308938 1101 78-79 8.977710531889747 1101 80-81 8.795276247561594 1101 82-83 8.507900075256464 1101 84-85 8.430647539051186 1101 86-87 8.381730364635258 1101 88-89 8.130652300551723 1101 90-91 7.74995438386633 1101 92-93 7.459829874093122 1101 94-95 7.050640774728654 1101 96-97 6.870254812077928 1101 98-99 6.150209464512384 1101 100-101 5.524187377836132 1102 1 7.703212686402562 1102 2 8.877139994837272 1102 3 8.32937614126644 1102 4 10.313073499417836 1102 5 11.127582289761964 1102 6 10.000676899662324 1102 7 9.802699977409194 1102 8 9.295668714534965 1102 9 9.22477580925256 1102 10-11 8.42971897029977 1102 12-13 8.570143671383018 1102 14-15 8.993469364619024 1102 16-17 9.791583134639549 1102 18-19 10.774319663887606 1102 20-21 10.753648696206454 1102 22-23 11.17959219988828 1102 24-25 10.975076939364858 1102 26-27 11.0251302871463 1102 28-29 10.896869643921988 1102 30-31 10.913605129403802 1102 32-33 10.834544037322587 1102 34-35 10.394713524531166 1102 36-37 10.46072788793126 1102 38-39 10.353393122446455 1102 40-41 10.151600478469895 1102 42-43 9.884661659683918 1102 44-45 9.921543198020945 1102 46-47 9.619767432882675 1102 48-49 9.120552294424712 1102 50-51 11.062110106906475 1102 52-53 10.306284152222524 1102 54-55 10.125162905854175 1102 56-57 9.843076768668954 1102 58-59 9.888406063475106 1102 60-61 9.858719249502983 1102 62-63 9.791444122430843 1102 64-65 9.504131878220743 1102 66-67 9.470600259228217 1102 68-69 9.217520934872388 1102 70-71 8.830078661707775 1102 72-73 8.688945632804757 1102 74-75 8.412739347489214 1102 76-77 8.302574577880215 1102 78-79 7.825433999451066 1102 80-81 7.452258928203557 1102 82-83 7.614386498462453 1102 84-85 7.38437999609263 1102 86-87 7.107347536272409 1102 88-89 6.829879480223703 1102 90-91 6.8406774945936615 1102 92-93 6.527662591996327 1102 94-95 6.041697360831458 1102 96-97 5.935902887259779 1102 98-99 5.477317659358308 1102 100-101 4.734634084558536 1103 1 8.042784654565295 1103 2 9.25410961606024 1103 3 8.566816538643618 1103 4 10.671348412589325 1103 5 11.526797516403494 1103 6 10.456407867019372 1103 7 10.192744834780239 1103 8 9.663742971034257 1103 9 9.603592595600386 1103 10-11 9.107953601455417 1103 12-13 8.962599595973781 1103 14-15 9.596968929268371 1103 16-17 10.460587486388814 1103 18-19 11.221020235334752 1103 20-21 11.542679024024174 1103 22-23 11.586873549308354 1103 24-25 11.498506195543452 1103 26-27 11.602057427634964 1103 28-29 11.201266784314008 1103 30-31 10.880760811410685 1103 32-33 11.209790377258077 1103 34-35 11.06459945036077 1103 36-37 11.150654330804556 1103 38-39 10.93081415089317 1103 40-41 10.657399625867939 1103 42-43 10.495294813722488 1103 44-45 10.344496017608297 1103 46-47 10.099257903491893 1103 48-49 10.153320338193488 1103 50-51 11.696572888160128 1103 52-53 10.92037100753235 1103 54-55 10.582939109701957 1103 56-57 10.374296868514055 1103 58-59 10.076896475217534 1103 60-61 10.10543220933387 1103 62-63 9.931240058953119 1103 64-65 9.596878259890154 1103 66-67 9.597769709034612 1103 68-69 9.40932007743808 1103 70-71 9.11730531553504 1103 72-73 9.042782912702275 1103 74-75 8.816597362553843 1103 76-77 8.583407321081317 1103 78-79 8.089484601166285 1103 80-81 7.84052570106474 1103 82-83 7.659859334954239 1103 84-85 7.466016864010495 1103 86-87 7.236815701201335 1103 88-89 6.7821001858695595 1103 90-91 6.446621239030966 1103 92-93 6.227432418462305 1103 94-95 6.1701504031461365 1103 96-97 5.811668869373062 1103 98-99 5.264112861363644 1103 100-101 4.659090258674194 1104 1 7.944153373758681 1104 2 9.36928139748159 1104 3 8.812488341144025 1104 4 10.710018375619235 1104 5 11.566968179344183 1104 6 10.710481825262189 1104 7 10.219067309100353 1104 8 9.733968499752969 1104 9 9.621712627502891 1104 10-11 9.119084150947753 1104 12-13 8.982359869003513 1104 14-15 9.57954353838089 1104 16-17 10.552000910393648 1104 18-19 11.23091701462668 1104 20-21 11.45385520537635 1104 22-23 11.656284254114642 1104 24-25 11.683703953678037 1104 26-27 11.66949353012565 1104 28-29 11.41093023133373 1104 30-31 11.206798083918173 1104 32-33 11.41800716634102 1104 34-35 11.166303864315626 1104 36-37 11.29883344429679 1104 38-39 10.668928143000137 1104 40-41 10.603879143475837 1104 42-43 10.23271507895727 1104 44-45 10.546362632767494 1104 46-47 10.054282667083797 1104 48-49 10.05859760954808 1104 50-51 11.591189586990284 1104 52-53 10.938352358588158 1104 54-55 10.585991755295876 1104 56-57 10.602863929172702 1104 58-59 10.486451988853965 1104 60-61 10.321091142853959 1104 62-63 10.080829909945681 1104 64-65 9.954258640534935 1104 66-67 9.775146060998107 1104 68-69 9.140719367719084 1104 70-71 9.082901926022432 1104 72-73 8.952045739309384 1104 74-75 8.895300759551667 1104 76-77 8.575094912656922 1104 78-79 8.283904823812767 1104 80-81 8.056831793945092 1104 82-83 7.872701041549233 1104 84-85 7.3764467283904125 1104 86-87 7.0328569894845625 1104 88-89 6.533798878365673 1104 90-91 6.323628589169617 1104 92-93 6.041840234577206 1104 94-95 5.7459334901699535 1104 96-97 5.335691736710071 1104 98-99 4.9697647269463605 1104 100-101 4.4816090491492115 1105 1 8.023364055620696 1105 2 9.044948803538105 1105 3 8.72093289194262 1105 4 10.699887207922316 1105 5 10.510393171468888 1105 6 10.603153349114653 1105 7 10.093229367692974 1105 8 9.639713586923385 1105 9 9.712961477626525 1105 10-11 9.181903051119118 1105 12-13 8.826436217990118 1105 14-15 9.482893268325476 1105 16-17 10.214885409473379 1105 18-19 10.663327237545609 1105 20-21 11.419097650778184 1105 22-23 11.66987961748907 1105 24-25 11.662939655769666 1105 26-27 11.65296948084504 1105 28-29 11.655496536448211 1105 30-31 11.267546869076451 1105 32-33 11.236425991690574 1105 34-35 11.17477553334566 1105 36-37 11.238923236697431 1105 38-39 10.924351659460989 1105 40-41 10.663823365708652 1105 42-43 10.433958529322751 1105 44-45 10.309517026279313 1105 46-47 10.290233974331073 1105 48-49 10.15805302228372 1105 50-51 11.407330106997692 1105 52-53 10.51826628650559 1105 54-55 10.40972740540484 1105 56-57 10.313017915235946 1105 58-59 10.270874148780074 1105 60-61 9.904724817611125 1105 62-63 10.038594017475962 1105 64-65 9.828584748329227 1105 66-67 9.511321968687685 1105 68-69 9.280656141006828 1105 70-71 9.163768367538191 1105 72-73 8.791462810542463 1105 74-75 8.47750395019699 1105 76-77 8.362386557447763 1105 78-79 7.94261107956604 1105 80-81 7.700544866646359 1105 82-83 7.676485784900775 1105 84-85 7.214132740399499 1105 86-87 6.978945093720766 1105 88-89 6.579974963658376 1105 90-91 6.094948104564432 1105 92-93 5.7634825462470065 1105 94-95 5.579524763479455 1105 96-97 5.397023496334317 1105 98-99 4.744119853637107 1105 100-101 4.276141033238879 1106 1 7.690987677595906 1106 2 9.022728411058345 1106 3 8.573106168553938 1106 4 10.413075076729783 1106 5 10.926064915670889 1106 6 10.131842796074991 1106 7 9.300143049592247 1106 8 9.139301894869043 1106 9 8.903188823127646 1106 10-11 8.450534861191844 1106 12-13 8.27080335581547 1106 14-15 8.92317687840736 1106 16-17 10.029366906202721 1106 18-19 10.8742023118789 1106 20-21 10.988016272982115 1106 22-23 10.939408916240271 1106 24-25 10.667055204006253 1106 26-27 10.861521239684986 1106 28-29 10.930563550765946 1106 30-31 10.480235675626929 1106 32-33 10.791176003126463 1106 34-35 10.608126249232395 1106 36-37 10.72261382724681 1106 38-39 10.49834141290319 1106 40-41 10.426286199064858 1106 42-43 10.06971810116302 1106 44-45 9.260118096678653 1106 46-47 8.98709367882767 1106 48-49 9.145999593803776 1106 50-51 11.045368623076554 1106 52-53 10.545251328360948 1106 54-55 9.934946288347064 1106 56-57 9.8001205180446 1106 58-59 9.411601013973797 1106 60-61 9.64662363285288 1106 62-63 9.52977054188015 1106 64-65 9.362309189114189 1106 66-67 9.164659418941564 1106 68-69 8.880846434223058 1106 70-71 8.607263633079564 1106 72-73 8.49437484567352 1106 74-75 8.179386297756569 1106 76-77 7.710945635257563 1106 78-79 7.505292567146666 1106 80-81 7.316317246683871 1106 82-83 7.101409164435102 1106 84-85 6.823385290603056 1106 86-87 6.437415886456693 1106 88-89 6.118599912196871 1106 90-91 5.937455309175382 1106 92-93 5.795730384863722 1106 94-95 5.536111836348949 1106 96-97 5.07805775826195 1106 98-99 4.824287275072541 1106 100-101 4.125646087902435 1107 1 7.219199948968075 1107 2 8.092128338542029 1107 3 8.00757075641954 1107 4 9.777529995753227 1107 5 10.490800835419499 1107 6 9.765328816540709 1107 7 9.691351251993343 1107 8 7.9738740486036335 1107 9 5.620712793798823 1107 10-11 7.646258614314078 1107 12-13 7.567123354203996 1107 14-15 8.141409998220425 1107 16-17 9.006168835942464 1107 18-19 10.261935270544598 1107 20-21 10.609702277333092 1107 22-23 9.920343772416242 1107 24-25 9.99473317113219 1107 26-27 10.184010362237558 1107 28-29 10.25233536609106 1107 30-31 9.927646540988302 1107 32-33 10.299242234695235 1107 34-35 10.48455048982542 1107 36-37 10.65142012810895 1107 38-39 10.224507515554066 1107 40-41 9.811530639480619 1107 42-43 9.819829192130705 1107 44-45 9.1884934290451 1107 46-47 9.356384026100248 1107 48-49 9.649721090862837 1107 50-51 11.024431752558465 1107 52-53 10.112657661452563 1107 54-55 9.839856952274225 1107 56-57 9.365839436745752 1107 58-59 9.397788267216889 1107 60-61 9.156151974646448 1107 62-63 9.25521692009303 1107 64-65 9.36298651165065 1107 66-67 9.316229800055094 1107 68-69 8.945946647743325 1107 70-71 8.663554423507408 1107 72-73 8.328112256035293 1107 74-75 8.098308524007475 1107 76-77 7.967192110158862 1107 78-79 7.599483305201614 1107 80-81 7.226246442558496 1107 82-83 6.986221528467011 1107 84-85 6.892011811431356 1107 86-87 6.456458570366129 1107 88-89 6.476623641146993 1107 90-91 6.310643408442166 1107 92-93 5.75024392124274 1107 94-95 5.526668600509774 1107 96-97 5.0675381587131625 1107 98-99 4.831475253478793 1107 100-101 4.13334852928508 1108 1 6.5619315149986654 1108 2 7.797863983701003 1108 3 7.826281221389049 1108 4 9.012157769296895 1108 5 10.65698806869036 1108 6 9.429953322627444 1108 7 8.77108778631602 1108 8 6.023851559871964 1108 9 7.060595181327205 1108 10-11 6.1590451359344165 1108 12-13 4.892383013174165 1108 14-15 7.171683555865876 1108 16-17 8.47385092077172 1108 18-19 8.078502864961319 1108 20-21 8.143110027197679 1108 22-23 9.15194904844046 1108 24-25 8.666851507796888 1108 26-27 7.025294340993494 1108 28-29 9.030826152501636 1108 30-31 9.526262475393107 1108 32-33 9.960827965894577 1108 34-35 9.42111154533384 1108 36-37 9.239631678768909 1108 38-39 8.722159480262334 1108 40-41 9.311609280138647 1108 42-43 8.482364353242694 1108 44-45 7.91993148756853 1108 46-47 8.824138959640653 1108 48-49 9.104720759323811 1108 50-51 10.591353604415646 1108 52-53 9.868441219992764 1108 54-55 9.009154211365189 1108 56-57 9.332925500283007 1108 58-59 9.33526799540282 1108 60-61 9.25158113993503 1108 62-63 9.443193357654241 1108 64-65 9.007043664585208 1108 66-67 8.566256163399498 1108 68-69 8.654341645338667 1108 70-71 8.729402668108117 1108 72-73 8.347914123518745 1108 74-75 8.290962807491717 1108 76-77 8.20545303217289 1108 78-79 7.69879407350936 1108 80-81 7.702706023157633 1108 82-83 7.701042691122451 1108 84-85 7.212178401804103 1108 86-87 6.920671878182938 1108 88-89 6.809857612633039 1108 90-91 6.515248825070882 1108 92-93 6.258654527136306 1108 94-95 5.892240709050061 1108 96-97 5.738230877556884 1108 98-99 5.335260790068718 1108 100-101 4.967794116141116 1109 1 6.257766524956846 1109 2 7.704037219301668 1109 3 7.656585477392312 1109 4 8.72442922927343 1109 5 9.771924423662636 1109 6 8.968006557793956 1109 7 7.393305149189018 1109 8 8.122422064797185 1109 9 7.166078907137628 1109 10-11 5.881116001377546 1109 12-13 6.29397750705353 1109 14-15 7.6005066364204446 1109 16-17 6.511046201985145 1109 18-19 5.599285694172025 1109 20-21 7.802251822310986 1109 22-23 8.637285115755194 1109 24-25 8.039603523555524 1109 26-27 8.683546367357057 1109 28-29 9.198591450315515 1109 30-31 6.813457681923911 1109 32-33 5.760042244972777 1109 34-35 7.331929044208874 1109 36-37 5.526722030899464 1109 38-39 5.979538994540505 1109 40-41 7.093748274056196 1109 42-43 5.899890791139569 1109 44-45 6.247879805601386 1109 46-47 5.659592084495955 1109 48-49 6.519146026266881 1109 50-51 8.313650310105475 1109 52-53 8.783643036831744 1109 54-55 8.913602178837817 1109 56-57 8.038194916843455 1109 58-59 7.844784254157986 1109 60-61 8.05636211223704 1109 62-63 8.532329356400371 1109 64-65 8.385005191484343 1109 66-67 8.034281097835628 1109 68-69 7.578988573288958 1109 70-71 7.178952068023298 1109 72-73 6.518946736224528 1109 74-75 5.541909459504659 1109 76-77 5.3590123511350995 1109 78-79 6.037288971740351 1109 80-81 6.025834026083599 1109 82-83 6.000525628483549 1109 84-85 5.796244944218802 1109 86-87 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-13.451139313431382 2313 52-53 -15.222367719258099 2313 54-55 -15.925847362446586 2313 56-57 -15.876863608939527 2313 58-59 -15.607287874915091 2313 60-61 -15.336709700480455 2313 62-63 -14.916737394627784 2313 64-65 -14.570230493425495 2313 66-67 -14.210419946137801 2313 68-69 -13.771217057840433 2313 70-71 -13.428450652634721 2313 72-73 -13.035466424167751 2313 74-75 -12.59918513081486 2313 76-77 -12.194609920297992 2313 78-79 -11.80010425825016 2313 80-81 -11.385270054903431 2313 82-83 -10.934305121279182 2313 84-85 -10.521217884000116 2313 86-87 -10.051314272273467 2313 88-89 -9.636003262406305 2313 90-91 -9.168100246380172 2313 92-93 -8.640936281802945 2313 94-95 -8.096427846190734 2313 96-97 -7.500831130626938 2313 98-99 -6.8474587566734915 2313 100-101 -5.985306293049945 2314 1 -18.346472639864412 2314 2 -17.10298587465594 2314 3 -17.710703355255585 2314 4 -19.205972542317838 2314 5 -18.550125560519586 2314 6 -19.494188949956754 2314 7 -19.65572996628077 2314 8 -20.074983819416673 2314 9 -21.279033399094576 2314 10-11 -21.588830218173232 2314 12-13 -21.47887918386707 2314 14-15 -21.391426296195817 2314 16-17 -20.28079182395601 2314 18-19 -19.308654830978245 2314 20-21 -18.98484086987503 2314 22-23 -18.607257750426395 2314 24-25 -18.412468605517557 2314 26-27 -18.145621617457873 2314 28-29 -17.9570554968531 2314 30-31 -17.900240514849262 2314 32-33 -17.746443044492583 2314 34-35 -17.397270576164427 2314 36-37 -16.97865601402303 2314 38-39 -16.81388080931903 2314 40-41 -16.678475705697046 2314 42-43 -16.50520253375762 2314 44-45 -16.120358093797538 2314 46-47 -15.76131901958503 2314 48-49 -15.488921041116857 2314 50-51 -13.451139313431382 2314 52-53 -15.222367719258099 2314 54-55 -15.925847362446586 2314 56-57 -15.876863608939527 2314 58-59 -15.607287874915091 2314 60-61 -15.336709700480455 2314 62-63 -14.916737394627784 2314 64-65 -14.570230493425495 2314 66-67 -14.210419946137801 2314 68-69 -13.771217057840433 2314 70-71 -13.428450652634721 2314 72-73 -13.035466424167751 2314 74-75 -12.59918513081486 2314 76-77 -12.194609920297992 2314 78-79 -11.80010425825016 2314 80-81 -11.385270054903431 2314 82-83 -10.934305121279182 2314 84-85 -10.521217884000116 2314 86-87 -10.051314272273467 2314 88-89 -9.636003262406305 2314 90-91 -9.168100246380172 2314 92-93 -8.640936281802945 2314 94-95 -8.096427846190734 2314 96-97 -7.500831130626938 2314 98-99 -6.8474587566734915 2314 100-101 -5.985306293049945 2315 1 -18.346472639864412 2315 2 -17.10298587465594 2315 3 -17.710703355255585 2315 4 -19.205972542317838 2315 5 -18.550125560519586 2315 6 -19.494188949956754 2315 7 -19.65572996628077 2315 8 -20.074983819416673 2315 9 -21.279033399094576 2315 10-11 -21.588830218173232 2315 12-13 -21.47887918386707 2315 14-15 -21.391426296195817 2315 16-17 -20.28079182395601 2315 18-19 -19.308654830978245 2315 20-21 -18.98484086987503 2315 22-23 -18.607257750426395 2315 24-25 -18.412468605517557 2315 26-27 -18.145621617457873 2315 28-29 -17.9570554968531 2315 30-31 -17.900240514849262 2315 32-33 -17.746443044492583 2315 34-35 -17.397270576164427 2315 36-37 -16.97865601402303 2315 38-39 -16.81388080931903 2315 40-41 -16.678475705697046 2315 42-43 -16.50520253375762 2315 44-45 -16.120358093797538 2315 46-47 -15.76131901958503 2315 48-49 -15.488921041116857 2315 50-51 -13.451139313431382 2315 52-53 -15.222367719258099 2315 54-55 -15.925847362446586 2315 56-57 -15.876863608939527 2315 58-59 -15.607287874915091 2315 60-61 -15.336709700480455 2315 62-63 -14.916737394627784 2315 64-65 -14.570230493425495 2315 66-67 -14.210419946137801 2315 68-69 -13.771217057840433 2315 70-71 -13.428450652634721 2315 72-73 -13.035466424167751 2315 74-75 -12.59918513081486 2315 76-77 -12.194609920297992 2315 78-79 -11.80010425825016 2315 80-81 -11.385270054903431 2315 82-83 -10.934305121279182 2315 84-85 -10.521217884000116 2315 86-87 -10.051314272273467 2315 88-89 -9.636003262406305 2315 90-91 -9.168100246380172 2315 92-93 -8.640936281802945 2315 94-95 -8.096427846190734 2315 96-97 -7.500831130626938 2315 98-99 -6.8474587566734915 2315 100-101 -5.985306293049945 2316 1 -18.346472639864412 2316 2 -17.10298587465594 2316 3 -17.710703355255585 2316 4 -19.205972542317838 2316 5 -18.550125560519586 2316 6 -19.494188949956754 2316 7 -19.65572996628077 2316 8 -20.074983819416673 2316 9 -21.279033399094576 2316 10-11 -21.588830218173232 2316 12-13 -21.47887918386707 2316 14-15 -21.391426296195817 2316 16-17 -20.28079182395601 2316 18-19 -19.308654830978245 2316 20-21 -18.98484086987503 2316 22-23 -18.607257750426395 2316 24-25 -18.412468605517557 2316 26-27 -18.145621617457873 2316 28-29 -17.9570554968531 2316 30-31 -17.900240514849262 2316 32-33 -17.746443044492583 2316 34-35 -17.397270576164427 2316 36-37 -16.97865601402303 2316 38-39 -16.81388080931903 2316 40-41 -16.678475705697046 2316 42-43 -16.50520253375762 2316 44-45 -16.120358093797538 2316 46-47 -15.76131901958503 2316 48-49 -15.488921041116857 2316 50-51 -13.451139313431382 2316 52-53 -15.222367719258099 2316 54-55 -15.925847362446586 2316 56-57 -15.876863608939527 2316 58-59 -15.607287874915091 2316 60-61 -15.336709700480455 2316 62-63 -14.916737394627784 2316 64-65 -14.570230493425495 2316 66-67 -14.210419946137801 2316 68-69 -13.771217057840433 2316 70-71 -13.428450652634721 2316 72-73 -13.035466424167751 2316 74-75 -12.59918513081486 2316 76-77 -12.194609920297992 2316 78-79 -11.80010425825016 2316 80-81 -11.385270054903431 2316 82-83 -10.934305121279182 2316 84-85 -10.521217884000116 2316 86-87 -10.051314272273467 2316 88-89 -9.636003262406305 2316 90-91 -9.168100246380172 2316 92-93 -8.640936281802945 2316 94-95 -8.096427846190734 2316 96-97 -7.500831130626938 2316 98-99 -6.8474587566734915 2316 100-101 -5.985306293049945 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 797063.0 3 33851.0 4 112874.0 5 63216.0 6 39275.0 7 38344.0 8 37467.0 9 37153.0 10 39518.0 11 40919.0 12 42449.0 13 42956.0 14 43799.0 15 45183.0 16 46247.0 17 46089.0 18 45368.0 19 46362.0 20 48601.0 21 51314.0 22 54445.0 23 57430.0 24 61325.0 25 65235.0 26 69420.0 27 74418.0 28 79026.0 29 85031.0 30 92023.0 31 97786.0 32 104875.0 33 110616.0 34 111131.0 35 100994.0 36 70231.0 37 27598.0 38 3127.0 39 19.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.68247834109213 21.57530024027936 14.137357885541629 22.604863533086885 2 14.592337262524117 21.376355206265725 42.277638724866144 21.75366880634401 3 18.014266161946217 27.79109646349261 30.579154387738743 23.61548298682243 4 11.580715636240662 17.900801641713038 41.182757785839456 29.33572493620684 5 11.788241746354005 38.91252883713091 36.38572948821483 12.913499928300261 6 27.76643237941505 40.44848153630668 18.69745275951053 13.087633324767733 7 25.126588320950354 33.67414403988725 24.26583235111206 16.93343528805033 8 23.984946868560773 37.200180224280984 21.339173119480737 17.475699787677502 9 25.5038237782036 16.86812712552005 22.42055614058307 35.207492955693276 10-11 23.38213562602069 27.960576433912294 29.708021115648958 18.949266824418057 12-13 24.10351585157604 25.706294888822683 29.79070065182272 20.399488607778554 14-15 21.6345023660711 27.172468609631473 27.6355417717756 23.557487252521824 16-17 20.392941131921614 30.14890391518932 28.536864264425315 20.921290688463756 18-19 20.10674763352922 28.94244703104078 30.91071688712525 20.040088448304743 20-21 21.116453943285194 28.404664328363744 30.738096120753056 19.74078560759801 22-23 20.792751917219146 28.244356348210196 30.515734091053993 20.447157643516665 24-25 20.511983697475234 28.42056246319446 30.951479116816127 20.11597472251418 26-27 20.349575067319243 28.576740612038105 30.74136998885898 20.33231433178367 28-29 20.388086685788288 28.584625698517524 30.82521401303117 20.20207360266302 30-31 20.777188291061137 28.39984494880012 30.699225235261192 20.123741524877552 32-33 20.54979644727681 28.794713740506428 30.386190931601075 20.26929888061569 34-35 20.74446907349725 28.59026580289577 30.641089179502227 20.024175944104748 36-37 20.826975785465287 28.296571885378796 30.571130897776243 20.30532143137967 38-39 20.662796272137264 28.69457992388146 30.643303643117275 19.999320160864006 40-41 20.811018647592803 28.512206294338764 30.663887961453902 20.012887096614527 42-43 20.75168945262031 28.507952032590843 30.278852171672018 20.461506343116824 44-45 20.671041833535046 29.00066408117134 30.029598471338375 20.298695613955235 46-47 20.84464627777601 28.690134238618555 29.802766225571066 20.662453258034375 48-49 20.8735226834258 28.81490381708592 29.67283848152028 20.638735017967996 50-51 21.02608576525774 29.069040684066653 29.171905712113972 20.732967838561635 52-53 20.442269493551912 29.491338108018887 29.233031881834144 20.833360516595057 54-55 20.56551473553692 29.28147549845997 29.406527842744413 20.7464819232587 56-57 20.61222803310234 29.430884378822565 29.37821413360625 20.57867345446884 58-59 20.480793922269047 29.318359969652718 29.558984040694497 20.641862067383734 60-61 20.555452376977428 29.633565563356157 29.28330967675753 20.52767238290889 62-63 20.43960484086222 29.38203927107442 29.446437821393474 20.731918066669895 64-65 20.281807990559308 29.169307427415713 29.518368978682773 21.03051560334221 66-67 20.368258583666893 29.239504350777977 29.15798367649215 21.234253389062978 68-69 20.367896938527604 29.279383087511725 29.26572984289176 21.086990131068916 70-71 20.663528477449074 28.804999761662053 29.44437957570597 21.0870921851829 72-73 20.636479423394476 29.111834099310347 29.257142358219674 20.9945441190755 74-75 20.357105145518545 29.502073175846576 28.973414915695628 21.16740676293925 76-77 20.37817328005156 29.314928480829305 29.254601303544437 21.052296935574695 78-79 20.242425152655542 29.36037006363687 29.077222463000073 21.319982320707513 80-81 20.344231878865305 29.32697680263433 28.779360871245903 21.549430447254466 82-83 20.677600819841572 28.97185533138929 29.125509652051147 21.225034196717996 84-85 20.66420594003147 29.128536219969597 28.781813481261604 21.42544435873733 86-87 20.41924118066549 29.23379918801591 28.994283937383162 21.352675693935435 88-89 20.650364040329176 29.1249262654715 28.85708080964337 21.367628884555952 90-91 20.639486572515015 29.035665871208145 29.05040962413561 21.27443793214123 92-93 20.649853883089232 29.154741687714974 28.66774997198348 21.527654457212314 94-95 20.329029430029617 28.945678167609746 28.84761993771195 21.877672464648686 96-97 20.454888094253214 28.961509630466413 28.82744110748789 21.756161167792477 98-99 20.294189249578135 29.066570676787013 28.539091836802754 22.1001482368321 100-101 20.634407185697555 29.109719278629647 28.414528960517355 21.841344575155443 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 180789.0 1 164407.5 2 109511.5 3 55372.5 4 34278.5 5 27230.0 6 25483.0 7 25674.0 8 26138.0 9 25590.5 10 23244.5 11 19505.5 12 15523.5 13 11999.5 14 9234.0 15 7570.5 16 7234.0 17 7979.5 18 9348.5 19 11057.0 20 12978.0 21 14805.0 22 16645.5 23 18963.0 24 21295.0 25 23907.0 26 27725.5 27 32079.0 28 36519.0 29 41584.5 30 47322.0 31 53861.5 32 61172.5 33 68627.5 34 75936.0 35 82522.5 36 89232.0 37 94847.5 38 96035.0 39 94468.5 40 92070.5 41 88870.5 42 85285.0 43 82985.0 44 82116.0 45 81536.5 46 78861.5 47 74849.5 48 71932.5 49 68012.0 50 63115.5 51 58162.5 52 52924.0 53 47350.5 54 42136.0 55 36874.0 56 30949.5 57 25257.5 58 20612.0 59 16897.5 60 13282.5 61 9845.5 62 7097.0 63 4886.0 64 3240.0 65 2180.0 66 1457.0 67 918.0 68 586.0 69 390.0 70 246.5 71 159.5 72 116.0 73 80.0 74 52.0 75 37.0 76 25.0 77 22.0 78 13.5 79 12.5 80 11.0 81 4.0 82 5.5 83 5.0 84 3.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.5 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 22.530645225528204 2 29.635700008573036 3 23.354770421543563 4 29.73729385056862 5 32.919206231449 6 25.394612758701463 7 24.34404467698896 8 20.29433187366721 9 12.017876465938386 10-11 15.707842437064134 12-13 15.770250082861423 14-15 19.95527508304706 16-17 23.344611037344006 18-19 27.365752682111182 20-21 29.43961714310016 22-23 29.061779181565413 24-25 27.553937554551844 26-27 29.018002698818474 28-29 25.941109998791674 30-31 27.859647263480426 32-33 26.8216687176697 34-35 24.847777997541495 36-37 27.886328304044376 38-39 28.01156549697615 40-41 24.99006000449578 42-43 25.873639536948094 44-45 24.54988190137769 46-47 27.898884087839182 48-49 23.241548978694997 50-51 28.797888333179202 52-53 22.3968518734782 54-55 23.54973946748626 56-57 25.664967135573434 58-59 24.94905119452082 60-61 24.48906060460824 62-63 24.31810618277846 64-65 22.833469129310398 66-67 23.342113381427836 68-69 24.353917168279228 70-71 22.820103294948186 72-73 22.68582053734704 74-75 19.928982191713317 76-77 22.23168593799468 78-79 21.343752383742558 80-81 22.377343155646493 82-83 23.260872059938343 84-85 20.166462016391375 86-87 21.740964054681115 88-89 20.580077211319917 90-91 21.707718229310466 92-93 21.69308669093668 94-95 20.729649673380862 96-97 19.6279640256543 98-99 19.03328565285688 100-101 20.69940778553101 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2962778.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.11674733041566 #Duplication Level Percentage of deduplicated Percentage of total 1 87.57819437526322 61.406981266643626 2 7.644473662454926 10.720112565287385 3 2.158026590290359 4.539414154911226 4 0.889975807945814 2.4960883542367673 5 0.46385407418563246 1.6261969458928938 6 0.26945189917759965 1.1335854439401833 7 0.18302243528588988 0.8983056495516666 8 0.132786062124563 0.7448421413591089 9 0.09400264805885698 0.5932043930099578 >10 0.5327870650425586 6.833884611164769 >50 0.037048684248505076 1.7739655202646796 >100 0.015662920927117345 1.9845716545003511 >500 4.588553539636974E-4 0.2169205152647002 >1k 2.0393571284251977E-4 0.24816450507486637 >5k 0.0 0.0 >10k+ 5.098392821062994E-5 4.783762278897792 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 133818 4.516639451217743 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 6.750421395055586E-5 0.0 5 0.0 0.0 0.0 6.750421395055586E-5 0.0 6 0.0 0.0 0.0 6.750421395055586E-5 0.0 7 0.0 0.0 0.0 6.750421395055586E-5 0.0 8 0.0 0.0 0.0 6.750421395055586E-5 0.0 9 0.0 0.0 0.0 6.750421395055586E-5 0.0 10-11 0.0 0.0 0.0 6.750421395055586E-5 0.0 12-13 0.0 0.0 0.0 6.750421395055586E-5 0.0 14-15 0.0 3.375210697527793E-5 0.0 6.750421395055586E-5 0.0 16-17 0.0 3.375210697527793E-5 0.0 6.750421395055586E-5 0.0 18-19 0.0 3.375210697527793E-5 0.0 6.750421395055586E-5 0.0 20-21 0.0 3.375210697527793E-5 0.0 8.438026743819483E-5 0.0 22-23 0.0 3.375210697527793E-5 0.0 1.3500842790111173E-4 0.0 24-25 0.0 3.375210697527793E-5 0.0 1.3500842790111173E-4 0.0 26-27 0.0 3.375210697527793E-5 0.0 2.8689290928986244E-4 0.0 28-29 0.0 3.375210697527793E-5 0.0 7.594224069437535E-4 0.0 30-31 0.0 3.375210697527793E-5 0.0 0.002058878525491954 0.0 32-33 0.0 3.375210697527793E-5 0.0 0.0051978244741928015 0.0 34-35 0.0 3.375210697527793E-5 0.0 0.010075003932120463 0.0 36-37 0.0 3.375210697527793E-5 0.0 0.019306205189858976 0.0 38-39 0.0 3.375210697527793E-5 0.0 0.034714042024073355 0.0 40-41 0.0 3.375210697527793E-5 0.0 0.05121882233498426 0.0 42-43 0.0 3.375210697527793E-5 0.0 0.06686292391802558 0.0 44-45 0.0 3.375210697527793E-5 0.0 0.08569659961023066 0.0 46-47 0.0 3.375210697527793E-5 0.0 0.11133132485795426 0.0 48-49 0.0 5.06281604629169E-5 0.0 0.14007124394740342 0.0 50-51 0.0 6.750421395055586E-5 0.0 0.17537594784354413 0.0 52-53 0.0 6.750421395055586E-5 0.0 0.21576034383946419 0.0 54-55 0.0 6.750421395055586E-5 0.0 0.26114005166772536 0.0 56-57 0.0 6.750421395055586E-5 0.0 0.3158184649676756 0.0 58-59 0.0 6.750421395055586E-5 0.0 0.377922341802187 0.0 60-61 0.0 6.750421395055586E-5 0.0 0.44562906839459454 0.0 62-63 0.0 6.750421395055586E-5 0.0 0.5139602089660447 0.0 64-65 0.0 6.750421395055586E-5 0.0 0.5847552533466902 0.0 66-67 0.0 6.750421395055586E-5 0.0 0.6666716169756897 0.0 68-69 0.0 6.750421395055586E-5 0.0 0.7626794852668678 0.0 70-71 0.0 6.750421395055586E-5 0.0 0.871209385245874 0.0 72-73 0.0 6.750421395055586E-5 0.0 0.9823719495689518 0.0 74-75 0.0 6.750421395055586E-5 0.0 1.0916781480083895 0.0 76-77 0.0 6.750421395055586E-5 0.0 1.2130169725845135 0.0 78-79 0.0 6.750421395055586E-5 0.0 1.3545733092388292 0.0 80-81 0.0 6.750421395055586E-5 0.0 1.5092760915600156 0.0 82-83 0.0 6.750421395055586E-5 0.0 1.6737669849040326 0.0 84-85 0.0 1.012563209258338E-4 0.0 1.8316930934413582 0.0 86-87 0.0 1.012563209258338E-4 0.0 1.9964033754807144 0.0 88-89 0.0 1.012563209258338E-4 0.0 2.1778884546867836 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2710 0.0 61.741703 1 GTATCAA 5380 0.0 47.127563 1 ATCAACG 6415 0.0 37.517868 3 TATCAAC 6555 0.0 36.715675 2 TCAACGC 6585 0.0 36.698795 4 CAACGCA 6575 0.0 36.05758 5 AACGCAG 7280 0.0 32.644234 6 CGCAGAG 8265 0.0 28.082684 8 ACGCAGA 8325 0.0 27.884798 7 GTACATG 6405 0.0 27.405474 1 ACATGGG 6375 0.0 26.67265 3 TACATGG 6465 0.0 26.669945 2 GCAGAGT 8925 0.0 25.09005 9 GTGTAGC 1005 0.0 24.002811 1 GAGTACT 5545 0.0 22.129337 12-13 GTGGTAT 1360 0.0 21.13389 1 CAGAGTA 8850 0.0 20.916656 10-11 CATGGGG 3685 0.0 20.810293 4 AGAGTAC 8065 0.0 20.257263 10-11 GAGTACA 4515 0.0 20.00715 1 >>END_MODULE