FastQCFastQC Report
Sat 18 Jun 2016
SRR1931010_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931010_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2962778
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96790.3266866434137151No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86880.29323830540121465No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48700.16437276096960352No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA30610.10331519945132575No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48750.062.0056841
GTATCAA93300.042.0263141
ATCAACG118100.032.957453
CAACGCA118950.032.822385
TCAACGC119950.032.528394
TATCAAC123100.031.620412
AACGCAG125100.031.4358046
ACGCAGA143300.027.2446337
CGCAGAG143850.027.1404678
ACATGGG99800.024.2385623
GCAGAGT158900.024.181799
TACATGG105450.023.75232
GTACATG108200.023.6761191
GTGATCG4100.023.140548
TGATCGC4200.022.5895759
GGCGTCG4200.022.5895758
GTGGTAT23050.022.4752521
GAGTACT113350.021.65789212-13
AGAGTAC150250.021.45370710-11
CAGAGTA156250.021.29781210-11
Produced by FastQC (version 0.11.5)