##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931010_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2962778 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.32187359295904 31.0 31.0 34.0 30.0 34.0 2 31.475033903991456 31.0 31.0 34.0 30.0 34.0 3 31.535736730865423 31.0 31.0 34.0 30.0 34.0 4 35.31761002680592 37.0 35.0 37.0 33.0 37.0 5 35.19702927455246 37.0 35.0 37.0 33.0 37.0 6 35.22155591812819 37.0 35.0 37.0 32.0 37.0 7 35.107659433140114 37.0 35.0 37.0 32.0 37.0 8 35.1136038542206 37.0 35.0 37.0 32.0 37.0 9 36.60735633921948 39.0 35.0 39.0 32.0 39.0 10-11 36.5440569627559 38.0 35.0 39.0 32.0 39.0 12-13 36.43216805309071 38.0 35.0 39.0 32.0 39.0 14-15 37.46736964430005 39.0 36.0 41.0 32.0 41.0 16-17 37.42700144931547 39.0 36.0 41.0 32.0 41.0 18-19 37.48104903573605 39.0 36.0 41.0 32.0 41.0 20-21 37.47337330032828 39.0 36.0 41.0 32.0 41.0 22-23 37.37233333040815 39.0 36.0 41.0 32.0 41.0 24-25 37.2492132383864 39.0 36.0 40.5 31.0 41.0 26-27 36.991708626160985 39.0 36.0 40.0 31.0 41.0 28-29 36.81417406231584 39.0 36.0 40.0 30.5 41.0 30-31 36.58137784876221 38.5 35.0 40.0 30.0 41.0 32-33 36.390792357712925 38.0 35.0 40.0 30.0 41.0 34-35 36.44479032853626 38.0 35.0 40.0 30.0 41.0 36-37 36.498291637105446 38.0 35.0 40.0 30.0 41.0 38-39 36.468451061807535 38.5 35.0 40.0 30.0 41.0 40-41 36.34789292346575 39.0 35.0 40.0 30.0 41.0 42-43 36.19773182465916 38.0 35.0 40.0 30.0 41.0 44-45 35.997175286167234 38.0 35.0 40.0 29.5 41.0 46-47 35.71387495114382 38.0 35.0 40.0 28.5 41.0 48-49 35.45788867745069 38.0 34.5 40.0 27.0 41.0 50-51 35.18469271069247 38.0 34.0 40.0 26.5 41.0 52-53 34.9785088521651 38.0 34.0 40.0 26.0 41.0 54-55 34.83306393526615 38.0 34.0 40.0 26.0 41.0 56-57 34.668134939573605 38.0 33.5 40.0 26.0 41.0 58-59 34.46331567873125 38.0 33.0 40.0 25.5 41.0 60-61 34.16844866540794 37.5 33.0 40.0 24.5 41.0 62-63 33.92553981432291 37.0 33.0 40.0 23.5 41.0 64-65 33.62439018380722 37.0 32.0 40.0 23.0 41.0 66-67 33.32050848899243 36.0 32.0 39.0 22.5 41.0 68-69 32.98387965618754 36.0 31.5 39.0 22.0 40.0 70-71 32.63684774897073 35.5 31.0 39.0 22.0 40.0 72-73 32.23343159696744 35.0 31.0 38.0 20.5 40.0 74-75 31.728409789731124 35.0 31.0 37.5 20.0 39.5 76-77 30.34458352937682 33.5 28.5 36.0 18.5 39.0 78-79 31.087496599475223 34.0 30.0 36.0 19.5 39.0 80-81 31.058662005725708 34.0 30.0 36.0 19.5 39.0 82-83 30.787000240990043 34.0 30.0 36.0 18.5 37.5 84-85 30.441261208230923 34.0 30.0 35.0 18.0 37.0 86-87 30.095593729938592 34.0 30.0 35.0 15.5 36.5 88-89 29.74475002852053 34.0 29.5 35.0 10.0 36.0 90-91 29.404675780635607 34.0 29.0 35.0 7.0 36.0 92-93 29.084306856605522 34.0 29.0 35.0 2.0 35.5 94-95 28.709203997059518 34.0 29.0 35.0 2.0 35.0 96-97 28.291166601075073 34.0 29.0 35.0 2.0 35.0 98-99 27.801202958844705 33.5 29.0 35.0 2.0 35.0 100-101 26.676148702332743 32.5 26.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.26652780550423216 1101 2 0.33292264640960667 1101 3 0.2428672449192142 1101 4 0.18392217506159625 1101 5 0.20490850257292692 1101 6 0.22157034085927307 1101 7 0.34131802408935386 1101 8 0.355574072725787 1101 9 0.47951145580858423 1101 10-11 0.45692076012734617 1101 12-13 0.39759325950789304 1101 14-15 0.3630051543111392 1101 16-17 0.5457956240528645 1101 18-19 0.47316867399842266 1101 20-21 0.4724103690060062 1101 22-23 0.46453810156396713 1101 24-25 0.5297949158558097 1101 26-27 0.4967754888767857 1101 28-29 0.5589632992324098 1101 30-31 0.5043218014519226 1101 32-33 0.5806493050729671 1101 34-35 0.5803666368276623 1101 36-37 0.5953828300060806 1101 38-39 0.7027266132754946 1101 40-41 0.807260734739117 1101 42-43 0.7469765234809529 1101 44-45 0.7702667509112615 1101 46-47 0.6800264967056364 1101 48-49 0.6891556528115501 1101 50-51 0.5823448365920427 1101 52-53 0.5029713966164309 1101 54-55 0.5034216464824155 1101 56-57 0.6391514604673318 1101 58-59 0.6066170486114473 1101 60-61 0.44360235380371194 1101 62-63 0.22850438199531453 1101 64-65 0.35090192014758514 1101 66-67 0.2420134935734879 1101 68-69 0.3558339094118068 1101 70-71 0.4125817615911842 1101 72-73 0.463939850946268 1101 74-75 0.43587457983339917 1101 76-77 0.4235928200076451 1101 78-79 0.4866883184208781 1101 80-81 0.5171704241917539 1101 82-83 0.513025046135386 1101 84-85 0.36797530859204386 1101 86-87 0.4285783481991423 1101 88-89 0.4196800952688484 1101 90-91 0.35432803812711455 1101 92-93 0.2724867910450719 1101 94-95 0.45949123525068103 1101 96-97 0.5189396263068922 1101 98-99 0.4772146678565896 1101 100-101 0.8697615177535383 1102 1 0.31229248176400404 1102 2 0.42781025473696843 1102 3 0.39719044206348286 1102 4 0.25567592915597714 1102 5 0.36302998457487945 1102 6 0.4338024864157646 1102 7 0.4541420020976119 1102 8 0.3934300322837174 1102 9 0.45959528641814984 1102 10-11 0.465150450877843 1102 12-13 0.4632674296923369 1102 14-15 0.6389951148519657 1102 16-17 0.5553075007546937 1102 18-19 0.6479111830776034 1102 20-21 0.536849578021787 1102 22-23 0.5185111732867185 1102 24-25 0.5151936309445091 1102 26-27 0.5870122284777963 1102 28-29 0.5875673942389668 1102 30-31 0.5885482495811303 1102 32-33 0.6265933362024896 1102 34-35 0.6778582836683853 1102 36-37 0.7483312673843585 1102 38-39 0.6337076485530773 1102 40-41 0.6480954526339389 1102 42-43 0.6818120609684897 1102 44-45 0.8268989199477375 1102 46-47 0.726518798735043 1102 48-49 0.6462131538393479 1102 50-51 0.5588911668623098 1102 52-53 0.5752083220270876 1102 54-55 0.6459420684424657 1102 56-57 0.5975175182947368 1102 58-59 0.5578852657368714 1102 60-61 0.4874017398373951 1102 62-63 0.32524760430112565 1102 64-65 0.4119143532853471 1102 66-67 0.4107147612940949 1102 68-69 0.5559615597648104 1102 70-71 0.45629789739465565 1102 72-73 0.4548191786065914 1102 74-75 0.554708738975183 1102 76-77 0.5748321174705922 1102 78-79 0.6857131928614351 1102 80-81 0.5368284571824589 1102 82-83 0.4158429533316301 1102 84-85 0.5600917931527363 1102 86-87 0.5330568906040192 1102 88-89 0.561717235574342 1102 90-91 0.3573994695831004 1102 92-93 0.38979486147355047 1102 94-95 0.5834891706008385 1102 96-97 0.4982551163224933 1102 98-99 0.6207290170185367 1102 100-101 0.8250231529800516 1103 1 0.35568543163146416 1103 2 0.4406328908620232 1103 3 0.4514923083163822 1103 4 0.2781206492883257 1103 5 0.2995832287059912 1103 6 0.3024761737522894 1103 7 0.3767685283508797 1103 8 0.3620887393406136 1103 9 0.4215114399040729 1103 10-11 0.4352975761138609 1103 12-13 0.5553164705590703 1103 14-15 0.6092407540969589 1103 16-17 0.5791477261881326 1103 18-19 0.5488857688794653 1103 20-21 0.5913951483838531 1103 22-23 0.4800968051338259 1103 24-25 0.5484378806697237 1103 26-27 0.6573689128894316 1103 28-29 0.5602696785339205 1103 30-31 0.5641676248375731 1103 32-33 0.6944028239472146 1103 34-35 0.7230582023092111 1103 36-37 0.6142726461675423 1103 38-39 0.5117909063992627 1103 40-41 0.6867119008052498 1103 42-43 0.6631053818724411 1103 44-45 0.6753633973556248 1103 46-47 0.6639561978597754 1103 48-49 0.5920849775587982 1103 50-51 0.5470177027802734 1103 52-53 0.46334286426752413 1103 54-55 0.47350801242412643 1103 56-57 0.5647892315730658 1103 58-59 0.5365164569634402 1103 60-61 0.4636761728491905 1103 62-63 0.4325816372999114 1103 64-65 0.3008600161649184 1103 66-67 0.32703704992938043 1103 68-69 0.3489119104615028 1103 70-71 0.36734666633196156 1103 72-73 0.3112542850017306 1103 74-75 0.3971330556152388 1103 76-77 0.4116131848572486 1103 78-79 0.4850497114444501 1103 80-81 0.43263825112234144 1103 82-83 0.40824079089970766 1103 84-85 0.3291431065410677 1103 86-87 0.22679940595550718 1103 88-89 0.3415251914646653 1103 90-91 0.36577321525924944 1103 92-93 0.27536639208911495 1103 94-95 0.4909293834701707 1103 96-97 0.5363223790959246 1103 98-99 0.5937651726482649 1103 100-101 0.8138750353554229 1104 1 0.3223605155820408 1104 2 0.3564429160622531 1104 3 0.3319332865223572 1104 4 0.1891422867267991 1104 5 0.32213330437551235 1104 6 0.27163686586811764 1104 7 0.327310711848817 1104 8 0.27618727404492205 1104 9 0.46187622376628923 1104 10-11 0.3920962070075049 1104 12-13 0.5348239153383858 1104 14-15 0.5661554273586447 1104 16-17 0.5900425343730049 1104 18-19 0.5281448894439293 1104 20-21 0.5520291462380911 1104 22-23 0.5603962417637192 1104 24-25 0.4140542581517792 1104 26-27 0.5493293814812006 1104 28-29 0.46562061904709395 1104 30-31 0.5994192313629654 1104 32-33 0.5313010275812431 1104 34-35 0.6164279565273887 1104 36-37 0.5542055566865116 1104 38-39 0.5730910670302976 1104 40-41 0.5333369607084819 1104 42-43 0.5982720053772255 1104 44-45 0.6977490348594912 1104 46-47 0.7453406682720924 1104 48-49 0.5757230574372514 1104 50-51 0.6176935812823032 1104 52-53 0.41921503827558837 1104 54-55 0.6149020888645396 1104 56-57 0.6380997235238723 1104 58-59 0.7028407143998336 1104 60-61 0.6859209140542788 1104 62-63 0.6597113965426473 1104 64-65 0.5410799245183355 1104 66-67 0.5685487496672224 1104 68-69 0.5156384445222528 1104 70-71 0.48540617122359464 1104 72-73 0.5051457028996538 1104 74-75 0.5100972896151639 1104 76-77 0.5551187699473878 1104 78-79 0.6800226140996379 1104 80-81 0.554474725378789 1104 82-83 0.49265123544414635 1104 84-85 0.5470784170614102 1104 86-87 0.6331265972526943 1104 88-89 0.7476669730838985 1104 90-91 0.7788683975328787 1104 92-93 0.6317605739248187 1104 94-95 0.8746298724182928 1104 96-97 0.8669549401327039 1104 98-99 1.0545239241141218 1104 100-101 1.0861707642219436 1105 1 0.3097515246471758 1105 2 0.27197725894990654 1105 3 0.31772192073252015 1105 4 0.15298613348999623 1105 5 0.2189338412147137 1105 6 0.28953271752651233 1105 7 0.31135977019393835 1105 8 0.3363946293277138 1105 9 0.4929533430934683 1105 10-11 0.44153769711225266 1105 12-13 0.45499863319088973 1105 14-15 0.3978671195331742 1105 16-17 0.4790645217436591 1105 18-19 0.4049511573593918 1105 20-21 0.3715190430610633 1105 22-23 0.382232421265023 1105 24-25 0.504464167201327 1105 26-27 0.38262979072657544 1105 28-29 0.4604258315222367 1105 30-31 0.2897249258072705 1105 32-33 0.40710404556724455 1105 34-35 0.593421600932075 1105 36-37 0.6386464947675137 1105 38-39 0.6686519340617494 1105 40-41 0.46498203613217015 1105 42-43 0.6446802210692582 1105 44-45 0.5763106136536322 1105 46-47 0.6903501191471193 1105 48-49 0.560991709166764 1105 50-51 0.6698718732159321 1105 52-53 0.5331191922049427 1105 54-55 0.6480613711360377 1105 56-57 0.6705440233931483 1105 58-59 0.7169338338990059 1105 60-61 0.8002799812494317 1105 62-63 0.6502745947449284 1105 64-65 0.6112149828661515 1105 66-67 0.5427123765681401 1105 68-69 0.4935772283604507 1105 70-71 0.5966329320884682 1105 72-73 0.5588472272433833 1105 74-75 0.6609974726086882 1105 76-77 0.7023700915587057 1105 78-79 0.8263803115696433 1105 80-81 0.7519759428949406 1105 82-83 0.8387295663154433 1105 84-85 0.7528326232694376 1105 86-87 0.6935273426041952 1105 88-89 0.7893100761781469 1105 90-91 0.7997046714442568 1105 92-93 0.8553825891926614 1105 94-95 0.9327978556849637 1105 96-97 1.0113483055031516 1105 98-99 1.0445829267801408 1105 100-101 1.1070450514336159 1106 1 0.2666437070725003 1106 2 0.2586914989206335 1106 3 0.22793645803639606 1106 4 0.07315538466789206 1106 5 0.1392796625403605 1106 6 0.13004596028758897 1106 7 0.17773459293763239 1106 8 0.2110828869554524 1106 9 0.2538572358705551 1106 10-11 0.2527961024917005 1106 12-13 0.31028247199058256 1106 14-15 0.2899823018212686 1106 16-17 0.3163468967493728 1106 18-19 0.3233656855145526 1106 20-21 0.2892583962471065 1106 22-23 0.25029563886714357 1106 24-25 0.3318952087822211 1106 26-27 0.3371556587106568 1106 28-29 0.41848996399559013 1106 30-31 0.45698076954726474 1106 32-33 0.4065661012829054 1106 34-35 0.3998892831058072 1106 36-37 0.3381222735516545 1106 38-39 0.29495814146305577 1106 40-41 0.33251828790473326 1106 42-43 0.3812759852154599 1106 44-45 0.44267445794255167 1106 46-47 0.35913608502242056 1106 48-49 0.36958097772721743 1106 50-51 0.30948991447662877 1106 52-53 0.31490090392901493 1106 54-55 0.36202733792621444 1106 56-57 0.5760693881980501 1106 58-59 0.5757403843770277 1106 60-61 0.4444257202366657 1106 62-63 0.323052143804901 1106 64-65 0.4672714304211567 1106 66-67 0.27629455487653587 1106 68-69 0.29701851649914346 1106 70-71 0.2357450495579343 1106 72-73 0.3080906287346252 1106 74-75 0.48730368001000457 1106 76-77 0.37648792697337186 1106 78-79 0.46193952661012716 1106 80-81 0.41446164803194563 1106 82-83 0.41442326742701496 1106 84-85 0.4720920350072362 1106 86-87 0.35804177469787746 1106 88-89 0.3968490725644074 1106 90-91 0.40994619744946803 1106 92-93 0.378866876279254 1106 94-95 0.6096269119952851 1106 96-97 0.5000304334936381 1106 98-99 0.4610789699620632 1106 100-101 0.6102164525589089 1107 1 0.11177141636264665 1107 2 0.16559384361485385 1107 3 0.13481745070419038 1107 4 0.09892828773774909 1107 5 0.07625653789286702 1107 6 0.15172350440168003 1107 7 0.19088764265159597 1107 8 0.1493384666831119 1107 9 0.10199135076862831 1107 10-11 0.14792582612395933 1107 12-13 0.16007922487109028 1107 14-15 0.08789604769186354 1107 16-17 0.24386693945343296 1107 18-19 0.14186913003957358 1107 20-21 0.13221172394027292 1107 22-23 0.14695646394167028 1107 24-25 0.14070130821014715 1107 26-27 0.26842761503971957 1107 28-29 0.18008834446452937 1107 30-31 0.2626441931231298 1107 32-33 0.22681708831053982 1107 34-35 0.10637425611362517 1107 36-37 0.16739499533072433 1107 38-39 0.06208317167818933 1107 40-41 -0.002934435485805409 1107 42-43 0.030825679035459075 1107 44-45 9.303398215294578E-4 1107 46-47 -0.021142901360626354 1107 48-49 -0.1125297506589007 1107 50-51 -0.06629084452748657 1107 52-53 -0.10405697617733978 1107 54-55 0.011332087744925445 1107 56-57 -0.02560724946209092 1107 58-59 -0.06165497055005176 1107 60-61 -0.02788070148599786 1107 62-63 -0.12760624391567177 1107 64-65 -0.12049849142236724 1107 66-67 -0.06559943029790105 1107 68-69 -0.09035699715809642 1107 70-71 0.07990506889561999 1107 72-73 -0.06422707330388988 1107 74-75 0.059522477850624256 1107 76-77 0.06036342048718879 1107 78-79 0.17479324508567018 1107 80-81 0.19969954183583027 1107 82-83 0.343713111114063 1107 84-85 0.2024865439106307 1107 86-87 0.12949854691607143 1107 88-89 0.12645859234524792 1107 90-91 0.23557799585411132 1107 92-93 0.17442505047955947 1107 94-95 0.21682486139501123 1107 96-97 0.3288552663032398 1107 98-99 0.32673081107075674 1107 100-101 0.5386782002021278 1108 1 0.09553079731936265 1108 2 0.2138405613784755 1108 3 0.1928719317537393 1108 4 0.1284555330558561 1108 5 0.14627997730271147 1108 6 0.16307172583460527 1108 7 0.16599035983362853 1108 8 0.20751078389326238 1108 9 0.24325064384753148 1108 10-11 0.22306994574005046 1108 12-13 0.28197004787079294 1108 14-15 0.3098361621567136 1108 16-17 0.30228736169834036 1108 18-19 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-1.588860905233254 2316 90-91 -1.564276886356076 2316 92-93 -1.5399265756874776 2316 94-95 -2.0970311388381297 2316 96-97 -2.1142042485489796 2316 98-99 -1.9993856222770567 2316 100-101 -2.2545285519146994 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 6.0 4 32.0 5 63.0 6 157.0 7 444.0 8 1024.0 9 2008.0 10 3637.0 11 6276.0 12 8713.0 13 11584.0 14 13895.0 15 17940.0 16 21673.0 17 26105.0 18 22149.0 19 15614.0 20 14161.0 21 14921.0 22 16443.0 23 19253.0 24 23468.0 25 30367.0 26 39169.0 27 50435.0 28 64367.0 29 81229.0 30 99327.0 31 120292.0 32 144989.0 33 176400.0 34 218939.0 35 273427.0 36 356214.0 37 490082.0 38 480287.0 39 97663.0 40 24.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.655082791471344 22.78108865654231 15.030797023179451 22.533031528806895 2 14.644219130474701 22.380747604623263 41.26866361842474 21.7063696464773 3 17.659900793597217 27.943958103251344 31.560273155136244 22.8358679480152 4 11.661597220437734 18.81550100960648 40.891293851611515 28.631607918344272 5 11.197165841859864 39.58357100955408 36.88601059704041 12.333252551545645 6 27.489030498589152 41.575422916469776 18.233538997421324 12.702007587519745 7 24.45735050010497 34.238609845219585 24.29395655023765 17.010083104437797 8 23.734513136937167 37.972115799055814 21.08150763833502 17.211863425672004 9 24.840909443772027 16.63992374723992 22.46506488167524 36.054101927312814 10-11 23.015392985907145 28.364393147242218 29.73960586989643 18.88060799695421 12-13 23.641832091368304 25.91120563201158 30.111756601405844 20.335205675214276 14-15 21.05832769110612 27.503393774356365 27.886547692739722 23.5517308417978 16-17 19.720141029803788 30.54071212895465 28.873273664108485 20.865873177133082 18-19 19.5229780969077 29.27392804995852 31.492099644320298 19.710994208813485 20-21 20.41823585837346 28.591899224309074 31.440037019310928 19.549827898006534 22-23 20.100237159539873 28.27288349919425 31.37217542954262 20.254703911723254 24-25 19.90319492850918 28.59850517659559 31.638172945613512 19.860126949281717 26-27 19.79970037582127 28.728868808184664 31.44589002337892 20.025540792615143 28-29 19.7376950137076 28.690069858553098 31.615556037461534 19.95667909027777 30-31 20.23893707632532 28.46633099542601 31.425653163195697 19.869078765052972 32-33 20.07623006687221 28.896718235024643 31.10148581282195 19.925565885281195 34-35 20.115135754060198 28.733911780114607 31.409261899746692 19.7416905660785 36-37 20.226461901949204 28.403679014429162 31.46546283014092 19.90439625348072 38-39 19.972576339034077 28.866111780529707 31.456187959240843 19.705123921195366 40-41 20.086754343581696 28.68796670372141 31.478396555073214 19.74688239762368 42-43 20.087470529371725 28.67875888591447 31.074519064831925 20.15925151988188 44-45 19.896887224874657 29.14881835318286 30.86924359718036 20.08505082476212 46-47 20.202153347666933 28.841771832505852 30.61719589529599 20.33887892453123 48-49 20.253433957551163 28.995099590465173 30.41987235475353 20.33159409723013 50-51 20.28956641797833 29.236986807671588 29.947038615956924 20.526408158393156 52-53 20.243448448660697 29.458999129720624 29.79464211344584 20.502910308172837 54-55 20.37568812720532 29.27568888675091 29.962925735851986 20.385697250191786 56-57 20.37324009981783 29.44899356881817 29.799209949918936 20.378556381445062 58-59 20.18729712755146 29.383618835171404 30.097793993414236 20.3312900438629 60-61 20.29022852357589 29.68986665459085 29.79145221389607 20.228452607937193 62-63 20.210348773966423 29.384712073062108 30.00997270405768 20.394966448913788 64-65 19.97159075048162 29.152302304732515 30.09426382535823 20.78184311942763 66-67 20.125936955381466 29.280316745909545 29.80189844503477 20.79184785367422 68-69 20.05540090278566 29.375117272632373 29.86281914863918 20.706662675942788 70-71 20.294594643515378 28.812406990790805 30.092176492888207 20.800821872805606 72-73 20.27816481741261 29.24400298918744 29.84028536902283 20.63754682437712 74-75 20.046665126954355 29.537113411655625 29.67649967556062 20.739721785829403 76-77 19.97825364602313 29.412298865249547 29.892730951436807 20.716716537290516 78-79 19.902991516820794 29.404675410594145 29.795578418144032 20.896754654441033 80-81 19.964735780778604 29.368870719745484 29.62604502904623 21.040348470429677 82-83 20.1909997981624 29.16804769037707 29.878057012709018 20.76289549875151 84-85 20.20723456161751 29.163980561486554 29.583249234333454 21.045535642562488 86-87 19.817212089464682 29.395351254802083 29.784732436922372 21.00270421881086 88-89 20.040705082228655 29.234332677979257 29.618988954611808 21.10597328518028 90-91 20.04537885981058 29.05207328800623 29.95343975065255 20.94910810153064 92-93 20.061688698585858 29.275716480442682 29.54240178132278 21.120193039648676 94-95 19.571769139039006 29.068154363197635 29.74177954508403 21.618296952679326 96-97 19.728281946295652 28.97494478997238 29.804217462198473 21.492555801533495 98-99 19.52348437851233 29.228700226611647 29.652778574702527 21.5950368201735 100-101 19.57630642592864 29.159069629921646 29.527845150733533 21.73677879341618 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 295.0 1 210.5 2 165.0 3 236.5 4 215.0 5 214.0 6 384.5 7 825.0 8 1395.0 9 1800.0 10 1804.5 11 1718.0 12 1933.5 13 2318.5 14 2714.0 15 3159.0 16 3727.0 17 4194.5 18 4755.0 19 5380.5 20 6183.0 21 7289.0 22 8670.5 23 10933.0 24 13647.5 25 16867.5 26 22658.5 27 30194.0 28 36575.0 29 44322.5 30 54017.5 31 63584.5 32 76277.5 33 90214.0 34 101771.0 35 111285.0 36 124999.5 37 137392.0 38 137250.5 39 133071.0 40 128204.0 41 121180.5 42 115010.0 43 114031.5 44 115163.5 45 116240.0 46 112354.5 47 104915.5 48 101894.5 49 97514.0 50 90535.5 51 82030.0 52 73226.0 53 65711.0 54 58665.5 55 49813.0 56 39666.0 57 30646.0 58 23972.5 59 19076.0 60 14159.5 61 9622.0 62 6189.0 63 3678.0 64 2123.0 65 1192.0 66 621.5 67 343.5 68 190.5 69 104.0 70 61.0 71 38.5 72 31.0 73 24.5 74 14.5 75 11.5 76 9.0 77 4.0 78 3.5 79 2.0 80 0.5 81 1.0 82 1.5 83 1.0 84 1.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0051978244741928015 2 0.006615412967154475 3 0.0022276390603683436 4 0.17517343520169248 5 0.0021601348464177876 6 6.075379255550028E-4 7 0.0 8 8.438026743819483E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 1.518844813887507E-4 24-25 6.750421395055586E-4 26-27 0.0022445151138559822 28-29 1.8563658836402862E-4 30-31 0.005299080795118635 32-33 0.018934932013130918 34-35 0.012218262725050612 36-37 9.450589953077821E-4 38-39 2.7001685580222345E-4 40-41 0.001501968760399868 42-43 0.00993999550421935 44-45 0.006632289020642113 46-47 0.009264953364713793 48-49 0.00912994493681268 50-51 0.18661539946631167 52-53 0.11420025395085288 54-55 0.016217887401621046 56-57 0.006109131362525306 58-59 0.01618413529464577 60-61 0.12474778738062725 62-63 0.24336956734524154 64-65 0.24984997188449487 66-67 0.26083628270494785 68-69 0.2562291201028224 70-71 0.2392855624012329 72-73 0.161453203716242 74-75 0.25923305762362214 76-77 0.2011794336261441 78-79 0.005079692099779329 80-81 5.06281604629169E-5 82-83 0.0 84-85 0.0 86-87 0.0 88-89 1.012563209258338E-4 90-91 0.005569097650920859 92-93 0.016369771883009795 94-95 0.0025989122370964008 96-97 3.375210697527793E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2962778.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.8360145973783 #Duplication Level Percentage of deduplicated Percentage of total 1 69.3799184549759 29.719591997022643 2 16.079942545450145 13.776013072038134 3 5.937036818376926 7.629569874514994 4 2.7835029603611994 4.769366937675123 5 1.5007458657905652 3.21429859069799 6 0.9354142116837488 2.404165009576811 7 0.6176039430163837 1.8518984062913746 8 0.42820345382632374 1.467402351900177 9 0.3038848876124121 1.1715495733519155 >10 1.7194579844688886 13.701010071040598 >50 0.17308809098599218 5.182368740070063 >100 0.1299654406974455 10.403433041640998 >500 0.008703434527954385 2.4251970174005866 >1k 0.0023736639621405376 1.6616268461173471 >5k 1.582442641427025E-4 0.6225084706612269 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9679 0.3266866434137151 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8688 0.29323830540121465 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4870 0.16437276096960352 No Hit ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 3061 0.10331519945132575 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 3.375210697527793E-5 0.0 9 0.0 0.0 0.0 3.375210697527793E-5 0.0 10-11 0.0 0.0 0.0 3.375210697527793E-5 0.0 12-13 0.0 0.0 0.0 3.375210697527793E-5 0.0 14-15 0.0 0.0 0.0 3.375210697527793E-5 0.0 16-17 0.0 0.0 0.0 3.375210697527793E-5 0.0 18-19 0.0 0.0 0.0 3.375210697527793E-5 0.0 20-21 0.0 0.0 0.0 3.375210697527793E-5 0.0 22-23 0.0 0.0 0.0 6.750421395055586E-5 0.0 24-25 0.0 0.0 0.0 6.750421395055586E-5 0.0 26-27 0.0 0.0 0.0 2.7001685580222345E-4 0.0 28-29 0.0 0.0 0.0 9.619350487954211E-4 0.0 30-31 0.0 0.0 0.0 0.0037464838742558505 0.0 32-33 0.0 0.0 0.0 0.010564409483261993 0.0 34-35 0.0 0.0 0.0 0.02107819080606107 0.0 36-37 0.0 0.0 0.0 0.03987811439129088 0.0 38-39 0.0 0.0 0.0 0.07162197100153977 0.0 40-41 0.0 0.0 0.0 0.10879991683480841 0.0 42-43 0.0 0.0 0.0 0.1456909697587872 0.0 44-45 0.0 0.0 0.0 0.1903112551801046 0.0 46-47 0.0 0.0 0.0 0.2465591414544053 0.0 48-49 0.0 0.0 0.0 0.30930430832144695 0.0 50-51 0.0 0.0 0.0 0.3790530373858588 0.0 52-53 0.0 0.0 0.0 0.4487511382898077 0.0 54-55 0.0 0.0 0.0 0.5280854657351985 0.0 56-57 0.0 0.0 0.0 0.626692246263473 0.0 58-59 0.0 0.0 0.0 0.7423607168677504 0.0 60-61 0.0 0.0 0.0 0.8684079603669259 0.0 62-63 0.0 0.0 0.0 0.993999550421935 0.0 64-65 0.0 0.0 0.0 1.1265778266208266 0.0 66-67 0.0 0.0 0.0 1.2774834969072946 0.0 68-69 0.0 0.0 0.0 1.4563696638762673 0.0 70-71 0.0 0.0 0.0 1.6609749363604023 0.0 72-73 0.0 0.0 0.0 1.8723137541861052 0.0 74-75 0.0 0.0 0.0 2.0820324708769946 0.0 76-77 0.0 0.0 0.0 2.31465199215061 0.0 78-79 0.0 0.0 0.0 2.5869471151736647 0.0 80-81 0.0 0.0 0.0 2.8902266724000247 0.0 82-83 0.0 0.0 0.0 3.2098253733489313 0.0 84-85 0.0 0.0 0.0 3.519889104077322 0.0 86-87 0.0 0.0 0.0 3.8425761228144664 0.0 88-89 0.0 0.0 0.0 4.21008593961478 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4875 0.0 62.005684 1 GTATCAA 9330 0.0 42.026314 1 ATCAACG 11810 0.0 32.95745 3 CAACGCA 11895 0.0 32.82238 5 TCAACGC 11995 0.0 32.52839 4 TATCAAC 12310 0.0 31.62041 2 AACGCAG 12510 0.0 31.435804 6 ACGCAGA 14330 0.0 27.244633 7 CGCAGAG 14385 0.0 27.140467 8 ACATGGG 9980 0.0 24.238562 3 GCAGAGT 15890 0.0 24.18179 9 TACATGG 10545 0.0 23.7523 2 GTACATG 10820 0.0 23.676119 1 GTGATCG 410 0.0 23.14054 8 TGATCGC 420 0.0 22.589575 9 GGCGTCG 420 0.0 22.589575 8 GTGGTAT 2305 0.0 22.475252 1 GAGTACT 11335 0.0 21.657892 12-13 AGAGTAC 15025 0.0 21.453707 10-11 CAGAGTA 15625 0.0 21.297812 10-11 >>END_MODULE