FastQCFastQC Report
Sat 18 Jun 2016
SRR1931009_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931009_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5168034
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2348164.543623358515056No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32050.052.236351
GTATCAA62050.046.2650761
ATCAACG73400.037.688463
CAACGCA74600.037.06865
TCAACGC76500.036.5517734
TATCAAC76950.035.6312872
AACGCAG83900.032.9927336
ACGCAGA94400.028.9422997
CGCAGAG95700.028.0149828
GTACATG98800.025.4696161
ACATGGG95800.025.1875723
TACATGG97450.025.0271052
GCAGAGT109250.023.618499
GGCGTCG6850.020.0216928
GAGTACA67250.019.931111
AGAGTAC98300.019.23758310-11
CATGGGG61300.019.1023884
GAGTACT66200.019.01203712-13
TACGGGC7200.018.9274184
CAGAGTA108150.018.75407210-11
Produced by FastQC (version 0.11.5)