##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931009_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5168034 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 20.857733327605818 28.0 2.0 31.0 2.0 33.0 2 19.55504143355094 27.0 2.0 31.0 2.0 33.0 3 20.208158460257806 27.0 2.0 31.0 2.0 33.0 4 21.699935217144468 30.0 2.0 35.0 2.0 37.0 5 21.024344073587752 30.0 2.0 35.0 2.0 37.0 6 22.012303905121367 30.0 2.0 35.0 2.0 37.0 7 22.20099422720516 30.0 2.0 35.0 2.0 37.0 8 22.62360503046226 30.0 2.0 35.0 2.0 37.0 9 23.92267272235438 32.0 2.0 37.0 2.0 39.0 10-11 24.269052699730693 32.0 2.0 37.0 2.0 39.0 12-13 24.173362249551765 32.0 2.0 37.0 2.0 39.0 14-15 24.120352536380373 32.0 2.0 38.0 2.0 40.0 16-17 22.94432283146744 30.5 2.0 38.0 2.0 40.0 18-19 21.879941772828893 28.0 2.0 37.0 2.0 40.0 20-21 21.53452328293506 27.0 2.0 37.0 2.0 40.0 22-23 21.160035324844998 27.0 2.0 37.0 2.0 39.5 24-25 20.991567586436155 27.0 2.0 37.0 2.0 39.0 26-27 20.726575134761113 26.5 2.0 37.0 2.0 39.0 28-29 20.519234490330366 25.0 2.0 36.0 2.0 39.0 30-31 20.511470125777038 25.0 2.0 36.0 2.0 39.0 32-33 20.38984524869612 25.0 2.0 36.0 2.0 39.0 34-35 20.077569149119377 24.5 2.0 36.0 2.0 39.0 36-37 19.63635388234675 24.0 2.0 36.0 2.0 39.0 38-39 19.511561843439885 24.0 2.0 35.0 2.0 39.0 40-41 19.384733246723997 23.5 2.0 35.0 2.0 39.0 42-43 19.253381846946056 23.5 2.0 35.0 2.0 38.5 44-45 18.875742400301547 21.0 2.0 35.0 2.0 38.0 46-47 18.581434835761527 19.0 2.0 35.0 2.0 38.0 48-49 18.345132888057627 18.0 2.0 34.5 2.0 38.0 50-51 16.154184066900488 10.0 2.0 32.5 2.0 37.5 52-53 18.041941674532325 17.5 2.0 33.5 2.0 38.0 54-55 18.78785104741958 21.0 2.0 35.0 2.0 39.0 56-57 18.763229982620082 21.0 2.0 35.5 2.0 39.0 58-59 18.491967158110803 19.5 2.0 35.0 2.0 39.0 60-61 18.210768640453992 18.0 2.0 35.0 2.0 39.0 62-63 17.770275601902 13.5 2.0 34.0 2.0 38.0 64-65 17.425689149877883 10.5 2.0 34.0 2.0 38.0 66-67 17.069097358879603 8.0 2.0 34.0 2.0 38.0 68-69 16.627256902721616 7.5 2.0 33.0 2.0 37.5 70-71 16.28602801374759 4.5 2.0 32.5 2.0 37.0 72-73 15.87990587910219 2.0 2.0 32.0 2.0 36.0 74-75 15.423707545267696 2.0 2.0 31.5 2.0 36.0 76-77 15.011055461322428 2.0 2.0 31.0 2.0 35.5 78-79 14.627192371412416 2.0 2.0 31.0 2.0 35.0 80-81 14.192596933379308 2.0 2.0 30.5 2.0 34.5 82-83 13.731749636322052 2.0 2.0 30.0 2.0 34.0 84-85 13.325361830049879 2.0 2.0 29.5 2.0 34.0 86-87 12.844199167420339 2.0 2.0 29.0 2.0 34.0 88-89 12.425496910430542 2.0 2.0 29.0 2.0 34.0 90-91 11.938630337958303 2.0 2.0 28.5 2.0 34.0 92-93 11.393484350141659 2.0 2.0 26.5 2.0 34.0 94-95 10.822850139917811 2.0 2.0 25.5 2.0 33.0 96-97 10.186063694627396 2.0 2.0 24.5 2.0 33.0 98-99 9.51189910902289 2.0 2.0 21.0 2.0 32.0 100-101 8.590574288017454 2.0 2.0 2.0 2.0 31.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.931286179186959 1101 2 9.55943385617925 1101 3 8.930934766766288 1101 4 11.100924168121754 1101 5 11.808164984443014 1101 6 10.943006789711056 1101 7 10.596582630391811 1101 8 10.012648020744336 1101 9 9.800232231450469 1101 10-11 9.391798786959015 1101 12-13 9.229507228866108 1101 14-15 9.961404903131882 1101 16-17 10.950249026417929 1101 18-19 11.82715780895536 1101 20-21 11.94267280233689 1101 22-23 12.140831437389384 1101 24-25 12.059976683031895 1101 26-27 11.968794886900465 1101 28-29 11.943564405967063 1101 30-31 11.63871903346265 1101 32-33 11.574011520395686 1101 34-35 11.387827213616063 1101 36-37 11.998521074077356 1101 38-39 11.643295435405054 1101 40-41 11.356976687450814 1101 42-43 11.048342367747477 1101 44-45 11.233801561813717 1101 46-47 11.007489154046066 1101 48-49 11.211432738433434 1101 50-51 12.716811760174206 1101 52-53 11.915210723131967 1101 54-55 11.74418953910958 1101 56-57 11.72539825753999 1101 58-59 11.53808838598949 1101 60-61 11.162749228413862 1101 62-63 11.285476146246818 1101 64-65 11.069083983216249 1101 66-67 10.686109872100364 1101 68-69 10.779154267232371 1101 70-71 10.5726689636395 1101 72-73 10.487844489218068 1101 74-75 10.1481285429046 1101 76-77 10.049791062660399 1101 78-79 9.745616202201642 1101 80-81 9.465409227677467 1101 82-83 9.015143833049715 1101 84-85 8.799744041574153 1101 86-87 8.603833491630311 1101 88-89 8.391992246115764 1101 90-91 7.927200827002446 1101 92-93 7.719270292089982 1101 94-95 7.262263854627731 1101 96-97 6.95914371676494 1101 98-99 6.687231462070834 1101 100-101 6.020857113704594 1102 1 7.870031948973917 1102 2 9.063912291410404 1102 3 8.564081001690333 1102 4 10.479919949089894 1102 5 11.372649830217274 1102 6 10.43220800622342 1102 7 10.12146895506346 1102 8 9.55110606150346 1102 9 9.337862864901577 1102 10-11 8.517058190331255 1102 12-13 8.87927876009142 1102 14-15 9.33154593508024 1102 16-17 10.2250022950495 1102 18-19 11.230718269321802 1102 20-21 11.182002229711397 1102 22-23 11.365074098808353 1102 24-25 11.181227260726185 1102 26-27 11.347624440995009 1102 28-29 11.088110599148031 1102 30-31 11.01171760227163 1102 32-33 10.998105099416811 1102 34-35 10.843006708823065 1102 36-37 10.982884939485768 1102 38-39 11.025738746823272 1102 40-41 10.658070579544972 1102 42-43 10.287697511835614 1102 44-45 10.47880096548413 1102 46-47 10.311467045126467 1102 48-49 9.843285683556203 1102 50-51 11.731814294574967 1102 52-53 11.029580030047903 1102 54-55 10.533476105294714 1102 56-57 10.423633494655544 1102 58-59 10.553762536592526 1102 60-61 10.566159488264478 1102 62-63 10.304739828283882 1102 64-65 9.965570483805127 1102 66-67 9.947908998477512 1102 68-69 9.78435798832902 1102 70-71 9.397419250623116 1102 72-73 9.313315962301237 1102 74-75 9.07572034039107 1102 76-77 8.9703226904911 1102 78-79 8.529474678281371 1102 80-81 8.00829501565248 1102 82-83 8.045042307936784 1102 84-85 7.7222372210544545 1102 86-87 7.267544599759773 1102 88-89 7.214698986130971 1102 90-91 6.928667425091211 1102 92-93 6.673570946561709 1102 94-95 6.3137990306194975 1102 96-97 6.1180972328732945 1102 98-99 5.560404457285289 1102 100-101 4.906024160531375 1103 1 8.02190953350101 1103 2 9.344312830116777 1103 3 8.68630665655892 1103 4 10.868666127531998 1103 5 11.415405631754805 1103 6 10.630518267791075 1103 7 10.338915124595285 1103 8 9.783324734223271 1103 9 9.525660610456303 1103 10-11 9.130989439536997 1103 12-13 8.920744799533264 1103 14-15 9.602932819157584 1103 16-17 10.511108439778639 1103 18-19 11.38021031069469 1103 20-21 11.68490916993807 1103 22-23 11.90763697831662 1103 24-25 11.710790087649233 1103 26-27 11.671301484478601 1103 28-29 11.258326366534387 1103 30-31 11.056605442377506 1103 32-33 11.389438653315931 1103 34-35 11.314678429513744 1103 36-37 11.35577711202956 1103 38-39 11.101672134494667 1103 40-41 10.88693825906142 1103 42-43 10.592506941534285 1103 44-45 10.60221099290148 1103 46-47 10.494555601559906 1103 48-49 10.585109996333099 1103 50-51 12.261150785942956 1103 52-53 11.37080742753228 1103 54-55 10.824986915209362 1103 56-57 10.51367151669167 1103 58-59 10.445736644214026 1103 60-61 10.522177757246649 1103 62-63 10.394771277167397 1103 64-65 10.12756404065486 1103 66-67 9.983771644419758 1103 68-69 9.830444114156691 1103 70-71 9.50075662279356 1103 72-73 9.392619259248525 1103 74-75 9.216247331946759 1103 76-77 8.835953282235513 1103 78-79 8.492789219701727 1103 80-81 8.307135219390998 1103 82-83 7.997738724692189 1103 84-85 7.697139500185569 1103 86-87 7.542993400013351 1103 88-89 7.132337493411834 1103 90-91 6.8989042630834465 1103 92-93 6.633959193472689 1103 94-95 6.316501472764804 1103 96-97 5.969349491481568 1103 98-99 5.4254202766725825 1103 100-101 4.804241192884431 1104 1 7.842292679350027 1104 2 9.436078906708918 1104 3 8.84334454043162 1104 4 10.679100357635544 1104 5 11.35187608214537 1104 6 10.559722656923576 1104 7 10.271245054575427 1104 8 9.711794696497172 1104 9 9.500270572854586 1104 10-11 8.888098567854847 1104 12-13 8.868622939648613 1104 14-15 9.352594861988745 1104 16-17 10.468602739186451 1104 18-19 11.218505365210707 1104 20-21 11.42493612716044 1104 22-23 11.579545603133692 1104 24-25 11.473314876834376 1104 26-27 11.567900977675105 1104 28-29 11.492537797543335 1104 30-31 11.216973857511721 1104 32-33 11.24377248308231 1104 34-35 11.230611842908313 1104 36-37 11.179773933266265 1104 38-39 10.9481785652602 1104 40-41 10.587936167408582 1104 42-43 10.198347602753653 1104 44-45 10.353674520799725 1104 46-47 10.156615035697715 1104 48-49 10.08591841275695 1104 50-51 11.90816267267044 1104 52-53 11.01820974325555 1104 54-55 10.657407108884737 1104 56-57 10.589854407887909 1104 58-59 10.511849823657165 1104 60-61 10.25035165760265 1104 62-63 10.214203853693515 1104 64-65 9.897214936439735 1104 66-67 9.804673878897479 1104 68-69 9.48594580308469 1104 70-71 9.410066826297747 1104 72-73 9.209418861692223 1104 74-75 9.06733005306708 1104 76-77 8.826494592039543 1104 78-79 8.43294172327628 1104 80-81 8.262260708910608 1104 82-83 8.081282773396909 1104 84-85 7.623383359498449 1104 86-87 7.341395354081346 1104 88-89 7.1152908873489356 1104 90-91 6.826657134218143 1104 92-93 6.4380290520611165 1104 94-95 6.181148929643362 1104 96-97 5.903299935915795 1104 98-99 5.507328887907029 1104 100-101 4.78671449462553 1105 1 7.894883607434458 1105 2 8.973551051568766 1105 3 8.52249834676332 1105 4 10.607009390917167 1105 5 10.467073847999814 1105 6 10.46263181690231 1105 7 9.978754819104164 1105 8 9.594522222113767 1105 9 9.457690486571426 1105 10-11 9.019887389393332 1105 12-13 8.580262821621066 1105 14-15 9.394831599109882 1105 16-17 10.384367316660622 1105 18-19 10.86939778492621 1105 20-21 11.198986431674346 1105 22-23 11.497801618486744 1105 24-25 11.687540872401161 1105 26-27 11.694082862099162 1105 28-29 11.446989423650383 1105 30-31 11.116192105116472 1105 32-33 11.01671871775882 1105 34-35 10.926444125928409 1105 36-37 11.197499223112803 1105 38-39 10.944366266643517 1105 40-41 10.695090003314515 1105 42-43 10.48914362020189 1105 44-45 10.282829663525579 1105 46-47 10.044228609241362 1105 48-49 9.98624614519876 1105 50-51 11.698177611127232 1105 52-53 10.609467569925993 1105 54-55 10.409977394018487 1105 56-57 10.391863084661228 1105 58-59 10.355793854637074 1105 60-61 10.228396229020351 1105 62-63 10.218407889353923 1105 64-65 9.809929497991725 1105 66-67 9.542956774170069 1105 68-69 9.311639497518897 1105 70-71 9.240487555110882 1105 72-73 9.110553220816252 1105 74-75 8.758695568807964 1105 76-77 8.364748197717743 1105 78-79 8.11339481712593 1105 80-81 7.814479754950115 1105 82-83 7.6689626737442556 1105 84-85 7.474988330690307 1105 86-87 7.180175263016324 1105 88-89 6.907316769242376 1105 90-91 6.5974081071998665 1105 92-93 6.290727037466025 1105 94-95 5.947127257287358 1105 96-97 5.632848332579519 1105 98-99 5.185563827803659 1105 100-101 4.740034871762207 1106 1 7.984254651428323 1106 2 9.30519398934436 1106 3 8.76131288075534 1106 4 10.499540565666255 1106 5 11.13786066258492 1106 6 10.365531943709719 1106 7 9.604679620393828 1106 8 9.243640539042168 1106 9 8.996923071401415 1106 10-11 8.736608599050598 1106 12-13 8.465786062752532 1106 14-15 8.9912066928031 1106 16-17 10.181322829151206 1106 18-19 11.332996240236444 1106 20-21 11.463799864045914 1106 22-23 11.510579490980565 1106 24-25 11.214918158238373 1106 26-27 11.40548641600206 1106 28-29 11.385370404758397 1106 30-31 11.039903577403958 1106 32-33 11.107556016555879 1106 34-35 11.008685506572238 1106 36-37 11.20760593502796 1106 38-39 10.890444030421254 1106 40-41 10.73762312842948 1106 42-43 10.562399715683735 1106 44-45 10.186832587729317 1106 46-47 10.00675476684236 1106 48-49 10.121172557557895 1106 50-51 11.90123916433809 1106 52-53 11.034812994855233 1106 54-55 10.658472451354196 1106 56-57 10.677453428467022 1106 58-59 10.259606231457173 1106 60-61 10.229671499288937 1106 62-63 10.008519046758206 1106 64-65 9.900767065804768 1106 66-67 9.658875256946825 1106 68-69 9.427632127826033 1106 70-71 9.095373826922618 1106 72-73 8.738902158757961 1106 74-75 8.560669759414528 1106 76-77 8.33552882493523 1106 78-79 8.055920594741849 1106 80-81 7.7292722881424805 1106 82-83 7.470742298540939 1106 84-85 7.173310696578271 1106 86-87 7.195168129048717 1106 88-89 6.785232826840046 1106 90-91 6.547061883629423 1106 92-93 6.233363246143073 1106 94-95 6.094637766564878 1106 96-97 5.613121087884933 1106 98-99 5.131904504264522 1106 100-101 4.661873858298295 1107 1 7.320487004143676 1107 2 8.298748705897456 1107 3 8.22736042795006 1107 4 9.811980946769495 1107 5 10.854597501321162 1107 6 9.702634386971337 1107 7 9.57785095516559 1107 8 8.206239580496504 1107 9 5.572312185186963 1107 10-11 7.561637035197663 1107 12-13 7.617357766203359 1107 14-15 8.036091992189842 1107 16-17 9.128624425771001 1107 18-19 10.435795859551096 1107 20-21 10.551117135164152 1107 22-23 10.043633462696487 1107 24-25 10.296968287115188 1107 26-27 10.358189678277775 1107 28-29 10.537873540238497 1107 30-31 10.27270251514658 1107 32-33 10.596162055814762 1107 34-35 10.587980028109499 1107 36-37 10.9741837319981 1107 38-39 10.560819460528865 1107 40-41 10.204919925853499 1107 42-43 9.906012415609457 1107 44-45 9.415944502686376 1107 46-47 9.622600454290673 1107 48-49 9.862048472145474 1107 50-51 11.308109592851213 1107 52-53 10.280375554627529 1107 54-55 10.014068282123343 1107 56-57 9.712939418347585 1107 58-59 9.889292341831712 1107 60-61 9.527022837400573 1107 62-63 9.701836639260048 1107 64-65 9.777320158690767 1107 66-67 9.743142269341316 1107 68-69 9.395917718779664 1107 70-71 9.232043894816144 1107 72-73 8.877854730133562 1107 74-75 8.77715066868137 1107 76-77 8.565105120954405 1107 78-79 8.249786595690082 1107 80-81 7.932428364655662 1107 82-83 7.826490380230746 1107 84-85 7.538417475367682 1107 86-87 7.260351107923135 1107 88-89 7.183740316752862 1107 90-91 6.9212633871673805 1107 92-93 6.418037759013922 1107 94-95 6.071600830833139 1107 96-97 5.76113611298287 1107 98-99 5.5401825365677215 1107 100-101 5.032788277559952 1108 1 6.643440302206702 1108 2 8.022893233304007 1108 3 7.95640599993753 1108 4 9.228811147552374 1108 5 10.8983675508331 1108 6 9.381124774964995 1108 7 8.652394145500175 1108 8 5.999972926958428 1108 9 6.874876668728046 1108 10-11 5.951692205401223 1108 12-13 4.832322551256475 1108 14-15 7.105146168512142 1108 16-17 8.372528886077632 1108 18-19 8.12420918981061 1108 20-21 8.367592140174178 1108 22-23 9.058332927772053 1108 24-25 8.664609260074556 1108 26-27 7.141195268605962 1108 28-29 9.043756166210212 1108 30-31 9.457878967229629 1108 32-33 10.047429446034318 1108 34-35 9.562542505151669 1108 36-37 9.247672603063378 1108 38-39 9.134055376990428 1108 40-41 9.31593612111936 1108 42-43 8.589805616376811 1108 44-45 8.168768033439207 1108 46-47 8.910365976536259 1108 48-49 9.387881899979746 1108 50-51 10.869688826989421 1108 52-53 10.31851036753434 1108 54-55 9.813533659043852 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2313 48-49 -16.210162592305867 2313 50-51 -14.06052287017599 2313 52-53 -15.909692000603439 2313 54-55 -16.64861201731065 2313 56-57 -16.621002520670128 2313 58-59 -16.35792328716226 2313 60-61 -16.077231316333222 2313 62-63 -15.645068600834893 2313 64-65 -15.291421853359626 2313 66-67 -14.931497131790689 2313 68-69 -14.493802931211206 2313 70-71 -14.156124810679565 2313 72-73 -13.76264189210489 2313 74-75 -13.320256671103182 2313 76-77 -12.910520221386294 2313 78-79 -12.526368233596024 2313 80-81 -12.098793106952884 2313 82-83 -11.633980680568962 2313 84-85 -11.226325997336351 2313 86-87 -10.751516702922217 2313 88-89 -10.326392967892017 2313 90-91 -9.845853647705725 2313 92-93 -9.318862003629862 2313 94-95 -8.756315911909235 2313 96-97 -8.125389357165021 2313 98-99 -7.444480600185976 2313 100-101 -6.534493080847936 2314 1 -18.67805234221382 2314 2 -17.398348244988064 2314 3 -18.043773404439936 2314 4 -19.544601123707043 2314 5 -18.84669882878656 2314 6 -19.824113832857037 2314 7 -20.004584684783286 2314 8 -20.440464819722592 2314 9 -21.731868935864707 2314 10-11 -22.09127514300208 2314 12-13 -22.004150358682526 2314 14-15 -21.949392762237768 2314 16-17 -20.778350195371956 2314 18-19 -19.721772152134132 2314 20-21 -19.366082061476344 2314 22-23 -19.008584905023067 2314 24-25 -18.834673122415577 2314 26-27 -18.575076708430277 2314 28-29 -18.356046488974574 2314 30-31 -18.354374351751357 2314 32-33 -18.23513244847591 2314 34-35 -17.930460724127126 2314 36-37 -17.490486708023813 2314 38-39 -17.364778494556038 2314 40-41 -17.24039685340522 2314 42-43 -17.11498448050155 2314 44-45 -16.736237348692118 2314 46-47 -16.439203452957823 2314 48-49 -16.210162592305867 2314 50-51 -14.06052287017599 2314 52-53 -15.909692000603439 2314 54-55 -16.64861201731065 2314 56-57 -16.621002520670128 2314 58-59 -16.35792328716226 2314 60-61 -16.077231316333222 2314 62-63 -15.645068600834893 2314 64-65 -15.291421853359626 2314 66-67 -14.931497131790689 2314 68-69 -14.493802931211206 2314 70-71 -14.156124810679565 2314 72-73 -13.76264189210489 2314 74-75 -13.320256671103182 2314 76-77 -12.910520221386294 2314 78-79 -12.526368233596024 2314 80-81 -12.098793106952884 2314 82-83 -11.633980680568962 2314 84-85 -11.226325997336351 2314 86-87 -10.751516702922217 2314 88-89 -10.326392967892017 2314 90-91 -9.845853647705725 2314 92-93 -9.318862003629862 2314 94-95 -8.756315911909235 2314 96-97 -8.125389357165021 2314 98-99 -7.444480600185976 2314 100-101 -6.534493080847936 2315 1 -18.67805234221382 2315 2 -17.398348244988064 2315 3 -18.043773404439936 2315 4 -19.544601123707043 2315 5 -18.84669882878656 2315 6 -19.824113832857037 2315 7 -20.004584684783286 2315 8 -20.440464819722592 2315 9 -21.731868935864707 2315 10-11 -22.09127514300208 2315 12-13 -22.004150358682526 2315 14-15 -21.949392762237768 2315 16-17 -20.778350195371956 2315 18-19 -19.721772152134132 2315 20-21 -19.366082061476344 2315 22-23 -19.008584905023067 2315 24-25 -18.834673122415577 2315 26-27 -18.575076708430277 2315 28-29 -18.356046488974574 2315 30-31 -18.354374351751357 2315 32-33 -18.23513244847591 2315 34-35 -17.930460724127126 2315 36-37 -17.490486708023813 2315 38-39 -17.364778494556038 2315 40-41 -17.24039685340522 2315 42-43 -17.11498448050155 2315 44-45 -16.736237348692118 2315 46-47 -16.439203452957823 2315 48-49 -16.210162592305867 2315 50-51 -14.06052287017599 2315 52-53 -15.909692000603439 2315 54-55 -16.64861201731065 2315 56-57 -16.621002520670128 2315 58-59 -16.35792328716226 2315 60-61 -16.077231316333222 2315 62-63 -15.645068600834893 2315 64-65 -15.291421853359626 2315 66-67 -14.931497131790689 2315 68-69 -14.493802931211206 2315 70-71 -14.156124810679565 2315 72-73 -13.76264189210489 2315 74-75 -13.320256671103182 2315 76-77 -12.910520221386294 2315 78-79 -12.526368233596024 2315 80-81 -12.098793106952884 2315 82-83 -11.633980680568962 2315 84-85 -11.226325997336351 2315 86-87 -10.751516702922217 2315 88-89 -10.326392967892017 2315 90-91 -9.845853647705725 2315 92-93 -9.318862003629862 2315 94-95 -8.756315911909235 2315 96-97 -8.125389357165021 2315 98-99 -7.444480600185976 2315 100-101 -6.534493080847936 2316 1 -18.67805234221382 2316 2 -17.398348244988064 2316 3 -18.043773404439936 2316 4 -19.544601123707043 2316 5 -18.84669882878656 2316 6 -19.824113832857037 2316 7 -20.004584684783286 2316 8 -20.440464819722592 2316 9 -21.731868935864707 2316 10-11 -22.09127514300208 2316 12-13 -22.004150358682526 2316 14-15 -21.949392762237768 2316 16-17 -20.778350195371956 2316 18-19 -19.721772152134132 2316 20-21 -19.366082061476344 2316 22-23 -19.008584905023067 2316 24-25 -18.834673122415577 2316 26-27 -18.575076708430277 2316 28-29 -18.356046488974574 2316 30-31 -18.354374351751357 2316 32-33 -18.23513244847591 2316 34-35 -17.930460724127126 2316 36-37 -17.490486708023813 2316 38-39 -17.364778494556038 2316 40-41 -17.24039685340522 2316 42-43 -17.11498448050155 2316 44-45 -16.736237348692118 2316 46-47 -16.439203452957823 2316 48-49 -16.210162592305867 2316 50-51 -14.06052287017599 2316 52-53 -15.909692000603439 2316 54-55 -16.64861201731065 2316 56-57 -16.621002520670128 2316 58-59 -16.35792328716226 2316 60-61 -16.077231316333222 2316 62-63 -15.645068600834893 2316 64-65 -15.291421853359626 2316 66-67 -14.931497131790689 2316 68-69 -14.493802931211206 2316 70-71 -14.156124810679565 2316 72-73 -13.76264189210489 2316 74-75 -13.320256671103182 2316 76-77 -12.910520221386294 2316 78-79 -12.526368233596024 2316 80-81 -12.098793106952884 2316 82-83 -11.633980680568962 2316 84-85 -11.226325997336351 2316 86-87 -10.751516702922217 2316 88-89 -10.326392967892017 2316 90-91 -9.845853647705725 2316 92-93 -9.318862003629862 2316 94-95 -8.756315911909235 2316 96-97 -8.125389357165021 2316 98-99 -7.444480600185976 2316 100-101 -6.534493080847936 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 1356275.0 3 54360.0 4 201746.0 5 110151.0 6 63742.0 7 62921.0 8 60214.0 9 58540.0 10 62313.0 11 65114.0 12 67030.0 13 68133.0 14 69311.0 15 70895.0 16 73842.0 17 75424.0 18 75930.0 19 79032.0 20 83481.0 21 88964.0 22 94487.0 23 100721.0 24 107070.0 25 115183.0 26 123180.0 27 132456.0 28 141147.0 29 152871.0 30 164792.0 31 178687.0 32 193238.0 33 204519.0 34 210014.0 35 195581.0 36 142422.0 37 57584.0 38 6624.0 39 40.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.6298716501777 20.908374155540614 14.094567444689913 23.367186749591777 2 14.562291859456392 21.095048401764554 41.970230371835044 22.372429366944008 3 18.29489422457155 26.75692850052566 30.17035778575353 24.777819489149252 4 12.038149102954803 17.50890279214207 40.41609420458955 30.03685390031358 5 12.473873811777919 38.42360437573289 36.01166885271824 13.090852959770954 6 28.992611367494796 39.30857495142341 18.641716300215307 13.05709738086649 7 26.025984237035942 33.0654746802841 23.6694328992547 17.23910818342526 8 24.17866578728239 36.76069463575592 21.3509200460704 17.70971953089129 9 25.49359166664649 17.20359793730704 21.78652857860699 35.516281817439484 10-11 23.592728947320648 27.533988699043377 29.53111991335734 19.342162440278635 12-13 24.37758457185275 24.923046997461974 29.514912414118726 21.184456016566553 14-15 21.76459312216682 26.629899015422758 27.1981989635545 24.40730889885592 16-17 21.150802006486366 29.618500750250874 27.9118870975187 21.318810145744056 18-19 20.886186363971525 28.78217533990688 29.751042188740623 20.580596107380973 20-21 21.825334875847634 28.231780425262443 29.48139856769542 20.4614861311945 22-23 21.479301340013198 28.005219508604362 29.230999513732474 21.284479637649966 24-25 21.21222718751453 28.17121589186534 29.564825202907247 21.051731717712883 26-27 21.114096525083855 28.489653339574538 29.324998113420094 21.07125202192151 28-29 21.121580947645374 28.489262668573524 29.465723088949918 20.923433294831185 30-31 21.581592505848498 28.20819246013408 29.376089635781987 20.834125398235432 32-33 21.32998936613126 28.385906013572104 29.18812666237069 21.095977957925946 34-35 21.406762632741756 28.359541677215294 29.361732437209525 20.87196325283342 36-37 21.470737427326267 28.189497308330385 29.25413639855816 21.085628865785186 38-39 21.3841078569171 28.448397215663135 29.29401602730402 20.873478900115742 40-41 21.6124909684183 28.239641933019673 29.277375196082943 20.87049190247909 42-43 21.594307180441984 28.156652245670134 29.02138863689328 21.227651936994597 44-45 21.524685751919137 28.537625325854286 28.865632484430588 21.07205643779599 46-47 21.557318651149764 28.2613503335675 28.680303519974004 21.501027495308733 48-49 21.523819771677854 28.347821166975645 28.90870612525374 21.219652936092757 50-51 21.644853457628393 28.545575067172408 28.45401843828721 21.355553036911992 52-53 21.166150730102917 28.773506055685168 28.595137511315915 21.465205702896 54-55 21.20981636244765 28.7379970129056 28.62074835670484 21.43143826794191 56-57 21.259984364155144 28.795814115416242 28.61900035806581 21.325201162362806 58-59 21.103427613647174 28.681900619987676 28.912651119410704 21.30202064695445 60-61 21.2089704244494 28.883323522298195 28.666662984584967 21.24104306866744 62-63 21.15019627721652 28.751306336481523 28.630462203501985 21.468035182799973 64-65 20.99212103996292 28.610450863258276 28.790712242257825 21.606715854520978 66-67 21.05565024011769 28.655844297097392 28.42230309615989 21.866202366625032 68-69 21.05332449655353 28.619244609158372 28.52857360785465 21.79885728643345 70-71 21.10975997346857 28.247016156284193 28.755874412872767 21.88734945737447 72-73 21.20451901559262 28.43578923500697 28.61575425030964 21.74393749909077 74-75 21.057707818492297 28.7661253325327 28.27268873511174 21.90347811386326 76-77 21.025690766294662 28.612463240665985 28.501717721227983 21.86012827181137 78-79 20.958445738347628 28.687337478836422 28.401183126335532 21.953033656480414 80-81 20.983868910745205 28.645614804712782 28.227813584810164 22.142702699731853 82-83 21.26981439064845 28.384930100308033 28.448508016744334 21.896747492299188 84-85 21.34257019164397 28.36854020443561 28.17947642288071 22.10941318103971 86-87 20.965004249916657 28.400035530566882 28.44727334490183 22.187686874614638 88-89 21.19630661104028 28.302828895159106 28.338834213693886 22.16203028010673 90-91 21.172028652951695 28.25322340753787 28.493186677556224 22.081561261954207 92-93 21.229770017694754 28.322167090726 28.20099608041675 22.24706681116249 94-95 20.98524717305537 28.21845519040922 28.334740856073747 22.46155678046166 96-97 20.991497931766382 28.219992523003825 28.332014809276302 22.4564947359535 98-99 20.847553684569426 28.221253740548963 28.080514762747793 22.850677812133817 100-101 21.1640779444978 28.280499183891884 27.93449965373157 22.620923217878744 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 316643.0 1 287044.5 2 190537.0 3 96555.5 4 59449.5 5 46543.5 6 42940.0 7 43276.5 8 45086.0 9 44918.0 10 41298.5 11 34936.5 12 27618.0 13 20596.5 14 14969.5 15 11480.5 16 10132.0 17 10602.5 18 12340.5 19 14676.0 20 17505.0 21 20529.5 22 23447.0 23 26509.5 24 29860.0 25 33458.5 26 37983.0 27 43474.0 28 49704.0 29 57409.0 30 66037.0 31 76042.0 32 88498.5 33 101980.5 34 115153.5 35 128426.0 36 143179.0 37 155214.5 38 160533.0 39 162347.5 40 162739.5 41 161661.0 42 160083.5 43 159620.0 44 159547.0 45 158857.5 46 155345.0 47 149557.5 48 144255.5 49 138053.5 50 129200.5 51 118921.0 52 107484.5 53 95597.5 54 84400.0 55 72423.0 56 59955.0 57 48297.5 58 38629.5 59 30967.5 60 24296.0 61 18085.0 62 12898.0 63 8995.0 64 6039.0 65 3989.0 66 2603.0 67 1659.0 68 1086.0 69 714.5 70 455.5 71 303.5 72 203.5 73 151.0 74 102.0 75 62.0 76 43.5 77 44.0 78 31.5 79 20.0 80 13.0 81 7.5 82 5.5 83 4.0 84 4.5 85 4.0 86 2.5 87 1.0 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 22.64323338430049 2 29.805860410361078 3 23.50878496542399 4 29.933607247939932 5 33.18633352644352 6 25.551341186996833 7 24.496278468756206 8 20.43457918427007 9 12.086859335677746 10-11 15.796471153247058 12-13 15.843326495143028 14-15 20.05429917837228 16-17 23.497455705593268 18-19 27.572380522264368 20-21 29.66008931055794 22-23 29.289029445239716 24-25 27.795182849029242 26-27 29.230109554232808 28-29 26.140133752990014 30-31 28.085776912458392 32-33 27.032794288892063 34-35 25.021565260600063 36-37 28.094610058679955 38-39 28.24791787360532 40-41 25.174911774961235 42-43 26.061544873737287 44-45 24.72652076205381 46-47 28.12323796631369 48-49 23.3942443103122 50-51 28.978679319834193 52-53 22.560358542532807 54-55 23.730706880024396 56-57 25.88474650128076 58-59 25.146312891904348 60-61 24.67685971106227 62-63 24.49480789019577 64-65 22.99244741810909 66-67 23.50131597431441 68-69 24.52903754116169 70-71 22.984543058346752 72-73 22.85488059869575 74-75 20.052064285954774 76-77 22.397066273170804 78-79 21.513625877848327 80-81 22.54577853009481 82-83 23.44163176945043 84-85 20.319361289031768 86-87 21.90528351787159 88-89 20.731616703760075 90-91 21.863555851219246 92-93 21.840055618829133 94-95 20.86104696679627 96-97 19.7751020987865 98-99 19.185400096052 100-101 20.845006050656785 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5168034.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.15265012918472 #Duplication Level Percentage of deduplicated Percentage of total 1 84.58978977588649 57.65018347097279 2 9.449325451838456 12.879931429518932 3 2.9144069984612018 5.958736815005211 4 1.1799478412847122 3.2166628959105514 5 0.573186770682731 1.9532098720508702 6 0.322691182490471 1.3195355556027581 7 0.1968965140131432 0.9393313461835707 8 0.13856429549992125 0.755481916128248 9 0.09300876249085711 0.5704914284089041 >10 0.48798925762897377 6.046184703513814 >50 0.03709647137242512 1.7600572020926912 >100 0.016396532910523162 1.9191582433039134 >500 5.542824774620897E-4 0.2326988394686382 >1k 1.166903700756586E-4 0.12974924619966233 >5k 0.0 0.0 >10k+ 2.9172592517299766E-5 4.668587035639293 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 234816 4.543623358515056 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 1.934971790046273E-5 3 0.0 0.0 0.0 0.0 1.934971790046273E-5 4 0.0 0.0 0.0 0.0 1.934971790046273E-5 5 0.0 0.0 0.0 0.0 1.934971790046273E-5 6 0.0 0.0 0.0 1.934971790046273E-5 1.934971790046273E-5 7 0.0 0.0 0.0 1.934971790046273E-5 1.934971790046273E-5 8 0.0 0.0 0.0 1.934971790046273E-5 1.934971790046273E-5 9 0.0 0.0 0.0 7.739887160185092E-5 1.934971790046273E-5 10-11 1.934971790046273E-5 0.0 0.0 1.06423448452545E-4 1.934971790046273E-5 12-13 1.934971790046273E-5 0.0 0.0 1.1609830740277637E-4 1.934971790046273E-5 14-15 1.934971790046273E-5 0.0 0.0 1.451228842534705E-4 1.934971790046273E-5 16-17 1.934971790046273E-5 0.0 0.0 1.934971790046273E-4 1.934971790046273E-5 18-19 3.869943580092546E-5 0.0 0.0 2.2252175585532136E-4 1.934971790046273E-5 20-21 3.869943580092546E-5 0.0 0.0 3.095954864074037E-4 1.934971790046273E-5 22-23 3.869943580092546E-5 0.0 0.0 3.579697811585605E-4 3.869943580092546E-5 24-25 3.869943580092546E-5 0.0 0.0 3.7731949905902326E-4 5.8049153701388184E-5 26-27 3.869943580092546E-5 0.0 0.0 4.450435117106427E-4 5.8049153701388184E-5 28-29 3.869943580092546E-5 0.0 0.0 7.643138570682779E-4 5.8049153701388184E-5 30-31 3.869943580092546E-5 0.0 0.0 0.0022542421354039078 5.8049153701388184E-5 32-33 3.869943580092546E-5 0.0 0.0 0.006530529791406172 5.8049153701388184E-5 34-35 3.869943580092546E-5 0.0 0.0 0.013012685288061186 5.8049153701388184E-5 36-37 3.869943580092546E-5 0.0 0.0 0.02369372956911661 5.8049153701388184E-5 38-39 3.869943580092546E-5 0.0 0.0 0.04036351154036526 6.772401265161956E-5 40-41 3.869943580092546E-5 0.0 0.0 0.057062318088464584 7.739887160185092E-5 42-43 3.869943580092546E-5 0.0 0.0 0.07574447072136135 7.739887160185092E-5 44-45 3.869943580092546E-5 0.0 0.0 0.09993161809693976 7.739887160185092E-5 46-47 3.869943580092546E-5 0.0 0.0 0.12897554466553432 7.739887160185092E-5 48-49 3.869943580092546E-5 0.0 0.0 0.15995444302417516 7.739887160185092E-5 50-51 3.869943580092546E-5 0.0 0.0 0.19367132646573146 7.739887160185092E-5 52-53 3.869943580092546E-5 0.0 0.0 0.23411223687769855 7.739887160185092E-5 54-55 3.869943580092546E-5 0.0 0.0 0.28125782454217596 7.739887160185092E-5 56-57 3.869943580092546E-5 0.0 0.0 0.3368592389291557 7.739887160185092E-5 58-59 3.869943580092546E-5 0.0 0.0 0.3981010960841202 7.739887160185092E-5 60-61 3.869943580092546E-5 1.934971790046273E-5 0.0 0.461277925029131 7.739887160185092E-5 62-63 3.869943580092546E-5 1.934971790046273E-5 0.0 0.5282376238236822 7.739887160185092E-5 64-65 3.869943580092546E-5 1.934971790046273E-5 0.0 0.6020761473318481 7.739887160185092E-5 66-67 3.869943580092546E-5 1.934971790046273E-5 1.934971790046273E-5 0.6885403617700657 7.739887160185092E-5 68-69 3.869943580092546E-5 1.934971790046273E-5 1.934971790046273E-5 0.7850470797986235 7.739887160185092E-5 70-71 3.869943580092546E-5 1.934971790046273E-5 1.934971790046273E-5 0.8904449932024441 7.739887160185092E-5 72-73 3.869943580092546E-5 1.934971790046273E-5 1.934971790046273E-5 1.0022766878081684 7.739887160185092E-5 74-75 3.869943580092546E-5 1.934971790046273E-5 2.9024576850694096E-5 1.1178912522634334 7.739887160185092E-5 76-77 3.869943580092546E-5 1.934971790046273E-5 3.869943580092546E-5 1.2477762336702893 7.739887160185092E-5 78-79 3.869943580092546E-5 1.934971790046273E-5 3.869943580092546E-5 1.3928604184879587 7.739887160185092E-5 80-81 3.869943580092546E-5 2.9024576850694096E-5 3.869943580092546E-5 1.5510443623242418 8.707373055208229E-5 82-83 3.869943580092546E-5 3.869943580092546E-5 3.869943580092546E-5 1.7190095885592085 9.674858950231365E-5 84-85 3.869943580092546E-5 3.869943580092546E-5 3.869943580092546E-5 1.8881938470219044 1.06423448452545E-4 86-87 5.8049153701388184E-5 3.869943580092546E-5 3.869943580092546E-5 2.0744252069549076 1.1609830740277637E-4 88-89 5.8049153701388184E-5 3.869943580092546E-5 3.869943580092546E-5 2.2777713923708705 1.1609830740277637E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3205 0.0 52.23635 1 GTATCAA 6205 0.0 46.265076 1 ATCAACG 7340 0.0 37.68846 3 CAACGCA 7460 0.0 37.0686 5 TCAACGC 7650 0.0 36.551773 4 TATCAAC 7695 0.0 35.631287 2 AACGCAG 8390 0.0 32.992733 6 ACGCAGA 9440 0.0 28.942299 7 CGCAGAG 9570 0.0 28.014982 8 GTACATG 9880 0.0 25.469616 1 ACATGGG 9580 0.0 25.187572 3 TACATGG 9745 0.0 25.027105 2 GCAGAGT 10925 0.0 23.61849 9 GGCGTCG 685 0.0 20.021692 8 GAGTACA 6725 0.0 19.93111 1 AGAGTAC 9830 0.0 19.237583 10-11 CATGGGG 6130 0.0 19.102388 4 GAGTACT 6620 0.0 19.012037 12-13 TACGGGC 720 0.0 18.927418 4 CAGAGTA 10815 0.0 18.754072 10-11 >>END_MODULE