FastQCFastQC Report
Sat 18 Jun 2016
SRR1931009_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931009_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5168034
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89620.17341217182394697No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64690.1251733250980934No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53200.053.7805331
GTATCAA111150.045.538671
ATCAACG138500.035.61573
CAACGCA138900.035.5540925
TCAACGC143450.034.48514
TATCAAC147750.033.709552
AACGCAG150550.033.211496
ACGCAGA170800.028.9684287
CGCAGAG170650.028.9104988
GCAGAGT198650.024.4056769
ACATGGG150950.023.768833
TACATGG157900.023.2958222
GTACATG165250.023.1235681
AGAGTAC187400.020.87130510-11
GAGTACT130500.019.8113712-13
CATGGGG83750.019.6891044
CAGAGTA199750.018.80905310-11
GAGTACA111150.018.6858221
GGCGTGC16900.018.5261758
GCGTGCG20350.017.9496029
Produced by FastQC (version 0.11.5)