##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931009_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5168034 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.317956306015013 31.0 31.0 34.0 30.0 34.0 2 31.48566708345959 31.0 31.0 34.0 30.0 34.0 3 31.561617241682235 31.0 31.0 34.0 30.0 34.0 4 35.337421348234166 37.0 35.0 37.0 33.0 37.0 5 35.201298017776196 37.0 35.0 37.0 33.0 37.0 6 35.21729520355323 37.0 35.0 37.0 32.0 37.0 7 35.09927875861498 37.0 35.0 37.0 32.0 37.0 8 35.10106686604616 37.0 35.0 37.0 32.0 37.0 9 36.59908081100085 39.0 35.0 39.0 32.0 39.0 10-11 36.542038326373245 38.0 35.0 39.0 32.0 39.0 12-13 36.42916048926923 38.0 35.0 39.0 32.0 39.0 14-15 37.49083461912209 39.0 36.0 41.0 32.0 41.0 16-17 37.449456795369386 39.0 36.0 41.0 32.0 41.0 18-19 37.46905360916743 39.0 36.0 41.0 32.0 41.0 20-21 37.44763463243469 39.0 36.0 41.0 32.0 41.0 22-23 37.34448157655309 39.0 36.0 40.5 32.0 41.0 24-25 37.220648993408325 39.0 36.0 40.5 31.0 41.0 26-27 36.975085012985595 39.0 36.0 40.0 31.0 41.0 28-29 36.79747414587443 39.0 36.0 40.0 30.0 41.0 30-31 36.59201603549822 38.0 35.0 40.0 30.0 41.0 32-33 36.44480376870585 38.0 35.0 40.0 30.0 41.0 34-35 36.54240335880143 38.0 35.0 40.0 30.0 41.0 36-37 36.62585491891114 39.0 35.0 40.0 30.0 41.0 38-39 36.613800721899274 39.0 35.0 40.0 30.0 41.0 40-41 36.518220952106745 39.0 35.0 40.0 30.0 41.0 42-43 36.39732836509977 39.0 35.0 40.0 30.0 41.0 44-45 36.248309705392806 38.5 35.0 40.0 30.0 41.0 46-47 36.047412130028555 38.0 35.0 40.0 29.5 41.0 48-49 35.858213394106926 38.0 35.0 40.0 29.0 41.0 50-51 35.61764938078968 38.0 34.5 40.0 27.5 41.0 52-53 35.42841687961031 38.0 34.0 40.0 27.0 41.0 54-55 35.288520160664575 38.0 34.0 40.0 27.0 41.0 56-57 35.12682946745319 38.0 34.0 40.0 27.0 41.0 58-59 34.92765740318272 38.0 34.0 40.0 26.5 41.0 60-61 34.63906516481896 38.0 33.0 40.0 26.0 41.0 62-63 34.39476085103155 37.0 33.0 40.0 26.0 41.0 64-65 34.09337680440957 37.0 33.0 40.0 26.0 41.0 66-67 33.7807783385326 36.0 32.0 39.0 25.0 41.0 68-69 33.43917077557926 36.0 32.0 39.0 24.5 40.0 70-71 33.08512676193694 35.5 31.5 39.0 24.0 40.0 72-73 32.672673980086046 35.0 31.0 38.0 22.5 40.0 74-75 32.163344029857384 35.0 31.0 37.5 22.0 39.5 76-77 30.741889178747662 33.5 29.5 36.0 20.5 38.5 78-79 31.495879864567456 34.0 30.5 36.0 22.0 39.0 80-81 31.47193555615153 34.0 31.0 36.0 22.0 38.5 82-83 31.20489251812198 34.0 31.0 36.0 21.5 37.0 84-85 30.867031447548527 34.0 30.5 35.0 20.5 37.0 86-87 30.53134025434043 34.0 30.0 35.0 20.0 36.5 88-89 30.196427403534884 34.0 30.0 35.0 18.5 36.0 90-91 29.872873707874213 34.0 30.0 35.0 17.5 36.0 92-93 29.566597375326864 34.0 29.5 35.0 12.5 35.5 94-95 29.20219719916703 34.0 29.0 35.0 4.5 35.0 96-97 28.799433691806207 34.0 29.0 35.0 2.0 35.0 98-99 28.320285818553053 34.0 29.0 35.0 2.0 35.0 100-101 27.192802911126357 33.0 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.35255770374253004 1101 2 0.3842694341125572 1101 3 0.2694709768272858 1101 4 0.18585053645566774 1101 5 0.3270640567846357 1101 6 0.36289765788239237 1101 7 0.37074380957817255 1101 8 0.3745967957450276 1101 9 0.470844885636005 1101 10-11 0.4602760054041326 1101 12-13 0.4357270368275934 1101 14-15 0.47783953121442124 1101 16-17 0.5885298538344514 1101 18-19 0.5226478755550801 1101 20-21 0.4772771360162906 1101 22-23 0.5015567625998756 1101 24-25 0.5294924866284063 1101 26-27 0.5324719607178139 1101 28-29 0.5839444399752338 1101 30-31 0.5481572868228497 1101 32-33 0.4839383209002861 1101 34-35 0.5033491515949606 1101 36-37 0.4656500723196686 1101 38-39 0.5002477036782551 1101 40-41 0.4805611266870571 1101 42-43 0.4283914334293115 1101 44-45 0.4467705865190652 1101 46-47 0.3928631635673909 1101 48-49 0.440424246918937 1101 50-51 0.4949822945917717 1101 52-53 0.42659839167789926 1101 54-55 0.41614842027636456 1101 56-57 0.5347177117085735 1101 58-59 0.5077970352604027 1101 60-61 0.4568564260870005 1101 62-63 0.2883841643890648 1101 64-65 0.46278365996224835 1101 66-67 0.5083319223503011 1101 68-69 0.45196118360382087 1101 70-71 0.4799817827139279 1101 72-73 0.5074794825940288 1101 74-75 0.4202641326262224 1101 76-77 0.36419235969181685 1101 78-79 0.3567172456452887 1101 80-81 0.4123530784369116 1101 82-83 0.4551958208875142 1101 84-85 0.419228231236783 1101 86-87 0.4553172103216241 1101 88-89 0.4417899807297836 1101 90-91 0.3906653100422126 1101 92-93 0.3733000122176158 1101 94-95 0.543846097760504 1101 96-97 0.6933613418568036 1101 98-99 0.6969128015894306 1101 100-101 0.884646473996689 1102 1 0.31269381597175894 1102 2 0.43653623197173275 1102 3 0.3995282244680567 1102 4 0.2280632174044257 1102 5 0.34682418320650754 1102 6 0.35574542984595325 1102 7 0.4794308409003847 1102 8 0.43851661923146423 1102 9 0.4966264501066817 1102 10-11 0.46299191398681216 1102 12-13 0.5149270606807832 1102 14-15 0.5566206335296684 1102 16-17 0.5758835667315694 1102 18-19 0.6353534229111091 1102 20-21 0.5495470646058251 1102 22-23 0.5380849317189416 1102 24-25 0.5932520465371667 1102 26-27 0.5300311091591539 1102 28-29 0.5899215260106843 1102 30-31 0.6028067941055255 1102 32-33 0.7187870768076721 1102 34-35 0.7660710980147485 1102 36-37 0.7618257361090386 1102 38-39 0.6864784534136348 1102 40-41 0.769931179014975 1102 42-43 0.7933615424085403 1102 44-45 0.8659217113457558 1102 46-47 0.819106510765387 1102 48-49 0.8673984705723541 1102 50-51 0.7794989694579684 1102 52-53 0.722567203640267 1102 54-55 0.859693897861824 1102 56-57 0.8033504024967755 1102 58-59 0.8433777857411968 1102 60-61 0.7517954513862861 1102 62-63 0.5893986701063696 1102 64-65 0.6357811106532409 1102 66-67 0.6681978227334469 1102 68-69 0.6799368682647255 1102 70-71 0.6499189444832396 1102 72-73 0.6842986605536865 1102 74-75 0.6795441391858432 1102 76-77 0.7155970885854721 1102 78-79 0.939649920034423 1102 80-81 0.7856870826410365 1102 82-83 0.5618411687712843 1102 84-85 0.7310068587842267 1102 86-87 0.7439127795927583 1102 88-89 0.7661433358291454 1102 90-91 0.7446014580512177 1102 92-93 0.6631598002224415 1102 94-95 0.7141391937546615 1102 96-97 0.8119493079256515 1102 98-99 0.9265101300328631 1102 100-101 1.2922213506310065 1103 1 0.3317144746600782 1103 2 0.3734713607982201 1103 3 0.40279464310776447 1103 4 0.2649229740809247 1103 5 0.3256431483585729 1103 6 0.39827825543976303 1103 7 0.4093320391130746 1103 8 0.4158713781209258 1103 9 0.5467395083316688 1103 10-11 0.494568243626027 1103 12-13 0.44738105226422675 1103 14-15 0.5629118709329575 1103 16-17 0.6054321518239476 1103 18-19 0.6723339064906355 1103 20-21 0.6222027862430366 1103 22-23 0.47092334317784434 1103 24-25 0.5602955739537876 1103 26-27 0.560908931780439 1103 28-29 0.5114469210206423 1103 30-31 0.5035081860071102 1103 32-33 0.6516196819817637 1103 34-35 0.6165909248340853 1103 36-37 0.6702367056782705 1103 38-39 0.6310263884942557 1103 40-41 0.6073892751670513 1103 42-43 0.7264405677706876 1103 44-45 0.7043780502750323 1103 46-47 0.7000685792926902 1103 48-49 0.6242961908569598 1103 50-51 0.5722406021998623 1103 52-53 0.6426998571635565 1103 54-55 0.72610501496181 1103 56-57 0.7546205912046844 1103 58-59 0.7856123727938424 1103 60-61 0.662791094116443 1103 62-63 0.5528412419326685 1103 64-65 0.5204965588765162 1103 66-67 0.5163094290220585 1103 68-69 0.4600876271717169 1103 70-71 0.4839148516002112 1103 72-73 0.4888500209771749 1103 74-75 0.5817772217168908 1103 76-77 0.6108571151994404 1103 78-79 0.6468938263567772 1103 80-81 0.694311168425056 1103 82-83 0.6996082396138057 1103 84-85 0.6237324968547568 1103 86-87 0.641098520817966 1103 88-89 0.6355404535747589 1103 90-91 0.665553199425414 1103 92-93 0.49905348605204125 1103 94-95 0.7133194485884857 1103 96-97 0.8477363260953048 1103 98-99 0.8185597558744071 1103 100-101 0.9519533047708499 1104 1 0.3206660096361631 1104 2 0.3797942516899191 1104 3 0.3325744708929399 1104 4 0.1814477320975172 1104 5 0.21662560039278134 1104 6 0.2734953897931618 1104 7 0.27615782742899597 1104 8 0.3802072996116337 1104 9 0.4635949675355775 1104 10-11 0.3752644482615821 1104 12-13 0.4553333935478534 1104 14-15 0.5386514788483083 1104 16-17 0.5808298528859481 1104 18-19 0.5988135673310495 1104 20-21 0.5515459862338261 1104 22-23 0.4661326796744589 1104 24-25 0.4269685934765235 1104 26-27 0.5822409455497919 1104 28-29 0.7078549385156379 1104 30-31 0.5957353212476661 1104 32-33 0.6078219877539723 1104 34-35 0.4831444172523618 1104 36-37 0.578498455596538 1104 38-39 0.560814062032847 1104 40-41 0.5249902775362187 1104 42-43 0.548686731554703 1104 44-45 0.5513364786480253 1104 46-47 0.3927989937007439 1104 48-49 0.36831767313745445 1104 50-51 0.33528481957934986 1104 52-53 0.23501002513845748 1104 54-55 0.3531546242146959 1104 56-57 0.5283216650684963 1104 58-59 0.5602515235394705 1104 60-61 0.46253983699084245 1104 62-63 0.355689325484974 1104 64-65 0.30845451049935235 1104 66-67 0.28902290303659584 1104 68-69 0.1905695730386654 1104 70-71 0.3010613650224485 1104 72-73 0.28305197703252105 1104 74-75 0.5201697565915353 1104 76-77 0.48723920989622016 1104 78-79 0.65136343948571 1104 80-81 0.5337401398364143 1104 82-83 0.45000156385345136 1104 84-85 0.5246824692570051 1104 86-87 0.5100237362401963 1104 88-89 0.584800554347833 1104 90-91 0.4252354667859599 1104 92-93 0.42389921065973724 1104 94-95 0.5872374405560024 1104 96-97 0.5679350627824569 1104 98-99 0.721139607593603 1104 100-101 1.0249506771047123 1105 1 0.26076427660716917 1105 2 0.28717151534202046 1105 3 0.32954477857530406 1105 4 0.12987069327898126 1105 5 0.2165372595327142 1105 6 0.30796505617989567 1105 7 0.2807695674398687 1105 8 0.28476272362589583 1105 9 0.3341406530200359 1105 10-11 0.3775691033632498 1105 12-13 0.3717533248287239 1105 14-15 0.46443751985388104 1105 16-17 0.31155241933476674 1105 18-19 0.347681693553028 1105 20-21 0.40887016900450845 1105 22-23 0.4259398272379826 1105 24-25 0.47603109388872866 1105 26-27 0.49095213883313704 1105 28-29 0.5149966010857625 1105 30-31 0.35355772217105397 1105 32-33 0.512748373957443 1105 34-35 0.5172353113542059 1105 36-37 0.5784061923328636 1105 38-39 0.5925668044004198 1105 40-41 0.4120334016911258 1105 42-43 0.43664698656786527 1105 44-45 0.4709372015643609 1105 46-47 0.46936365264982527 1105 48-49 0.3372796413037875 1105 50-51 0.331249846456835 1105 52-53 0.39807836666914653 1105 54-55 0.42889957023235326 1105 56-57 0.5374384938947401 1105 58-59 0.57065259243263 1105 60-61 0.5372604629259286 1105 62-63 0.4652850311958545 1105 64-65 0.4499278506856612 1105 66-67 0.5100812744421148 1105 68-69 0.34741116826146623 1105 70-71 0.24192148079375642 1105 72-73 0.3891737174717349 1105 74-75 0.5448353751549249 1105 76-77 0.4953524934943978 1105 78-79 0.5643094402064612 1105 80-81 0.4449787668618619 1105 82-83 0.5038223563830861 1105 84-85 0.5760033195813463 1105 86-87 0.6063627590951732 1105 88-89 0.6323041943730949 1105 90-91 0.6169061316430877 1105 92-93 0.5863711906397846 1105 94-95 0.6140646506239023 1105 96-97 0.7154426217401735 1105 98-99 0.7215575865588271 1105 100-101 0.8551011766071923 1106 1 0.27549670154078143 1106 2 0.23884239042747524 1106 3 0.31078131726770764 1106 4 0.1253551254921632 1106 5 0.22625563006255334 1106 6 0.21386918359124252 1106 7 0.20685053250780783 1106 8 0.2419252237393863 1106 9 0.3919770717985642 1106 10-11 0.3538434909231327 1106 12-13 0.3639963498327532 1106 14-15 0.4522755762672048 1106 16-17 0.441033326093482 1106 18-19 0.3698842047499795 1106 20-21 0.29072149328612085 1106 22-23 0.34839400288726097 1106 24-25 0.4160526353435827 1106 26-27 0.44607764992147025 1106 28-29 0.4373178323988185 1106 30-31 0.495276283702232 1106 32-33 0.42209076102368925 1106 34-35 0.5145528857312414 1106 36-37 0.4625755769016351 1106 38-39 0.5163363673267938 1106 40-41 0.48339777677876583 1106 42-43 0.5227773295195348 1106 44-45 0.4796466949030318 1106 46-47 0.4655447017516323 1106 48-49 0.43547190433685046 1106 50-51 0.3694762106284841 1106 52-53 0.38847254570129763 1106 54-55 0.578903185134628 1106 56-57 0.5982471525776774 1106 58-59 0.6133760631692411 1106 60-61 0.5820036215972664 1106 62-63 0.4089062755225541 1106 64-65 0.46177785342626265 1106 66-67 0.4359658914692801 1106 68-69 0.39174222807346837 1106 70-71 0.3952946217672135 1106 72-73 0.43548488624193027 1106 74-75 0.6582153054192545 1106 76-77 0.5632476048350696 1106 78-79 0.6193287908624008 1106 80-81 0.43560643495397855 1106 82-83 0.5454464596771516 1106 84-85 0.5988020710781718 1106 86-87 0.566304127061624 1106 88-89 0.5903718323892235 1106 90-91 0.5449728550048754 1106 92-93 0.6218861931567652 1106 94-95 0.786817114558108 1106 96-97 0.8527690433803592 1106 98-99 0.8193056033225581 1106 100-101 1.0142841044493736 1107 1 0.12421381216622507 1107 2 0.19413481385053544 1107 3 0.14377238867406916 1107 4 0.0811490930655836 1107 5 0.1500872190855631 1107 6 0.184481621667274 1107 7 0.1860799979115626 1107 8 0.15015120118582104 1107 9 0.13167741558615376 1107 10-11 0.15497237984298096 1107 12-13 0.17054977272564287 1107 14-15 0.21759438863373504 1107 16-17 0.28935354952971437 1107 18-19 0.21939298367968263 1107 20-21 0.21478380522157892 1107 22-23 0.20356553433650504 1107 24-25 0.1588013971129314 1107 26-27 0.25530996598781286 1107 28-29 0.20099307775231523 1107 30-31 0.32250828700827583 1107 32-33 0.2840315299264944 1107 34-35 0.27924536490722573 1107 36-37 0.25339645993707194 1107 38-39 0.31809041202564714 1107 40-41 0.26826638013816506 1107 42-43 0.19273212520794658 1107 44-45 0.2528494383714204 1107 46-47 0.25854515596715544 1107 48-49 0.3620063990954634 1107 50-51 0.20872194089476892 1107 52-53 0.1283661839809085 1107 54-55 0.18291989973521794 1107 56-57 0.25950548711744403 1107 58-59 0.24941772496458015 1107 60-61 0.2720575785540831 1107 62-63 0.13031783607873848 1107 64-65 0.06573175063359571 1107 66-67 0.057505740133763084 1107 68-69 0.0826272551630538 1107 70-71 0.10557005812670894 1107 72-73 0.07069312308141917 1107 74-75 0.23004898459650036 1107 76-77 0.20951054205896824 1107 78-79 0.3071433110663726 1107 80-81 0.2733907819142729 1107 82-83 0.35078441244371206 1107 84-85 0.26417244080363744 1107 86-87 0.33349757760213095 1107 88-89 0.3146947668569844 1107 90-91 0.38353489058028245 1107 92-93 0.4392426986125777 1107 94-95 0.46093208663936025 1107 96-97 0.4916367084588451 1107 98-99 0.5196438686835911 1107 100-101 0.8324675176393974 1108 1 0.06550881500256267 1108 2 0.16122804824515313 1108 3 0.07578622033556925 1108 4 -0.001438698304383479 1108 5 0.07804219458115824 1108 6 0.16907538130366362 1108 7 0.15656255670735675 1108 8 0.13086491942654277 1108 9 0.2067652719766997 1108 10-11 0.19290481006837013 1108 12-13 0.12535313576666596 1108 14-15 0.14039113615061893 1108 16-17 0.14851615069075308 1108 18-19 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-0.87154790284999 2316 68-69 -0.9242626221818426 2316 70-71 -1.0364026434482199 2316 72-73 -1.6811677231517628 2316 74-75 -2.82504846921983 2316 76-77 -1.8066081113151817 2316 78-79 -1.6400360320854155 2316 80-81 -1.3459616687298244 2316 82-83 -0.9305476210532682 2316 84-85 -0.9419095113870846 2316 86-87 -0.7456120743919961 2316 88-89 -0.852083314987226 2316 90-91 -0.7576342564537555 2316 92-93 -0.7076238265366435 2316 94-95 -1.3105800077031198 2316 96-97 -1.035523993539666 2316 98-99 -1.2189299806959433 2316 100-101 -1.6221731849247156 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 29.0 4 60.0 5 108.0 6 211.0 7 595.0 8 1645.0 9 3051.0 10 5325.0 11 8524.0 12 11858.0 13 15824.0 14 19172.0 15 23861.0 16 27841.0 17 31852.0 18 27509.0 19 22316.0 20 21773.0 21 23793.0 22 26656.0 23 31781.0 24 39708.0 25 51319.0 26 67658.0 27 87592.0 28 112799.0 29 142503.0 30 175758.0 31 213091.0 32 257864.0 33 314186.0 34 389736.0 35 486187.0 36 636801.0 37 879198.0 38 851897.0 39 157909.0 40 38.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.662305761569066 22.064351811627787 14.897719750199842 23.375622676603307 2 14.554627286542152 22.156467414893676 41.032992929326355 22.25591236923782 3 17.971424098781696 27.13808899168544 30.909044468752523 23.981442440780345 4 12.127575446281472 18.38076870893338 40.18820382610044 29.303452018684705 5 11.966444728287865 39.13630373043397 36.37013973236201 12.527111808916144 6 28.667386995231436 40.442224205361754 18.244230083057083 12.646158716349728 7 25.420092050477994 33.58795627118552 23.728965405413355 17.26298627292313 8 23.876681697649687 37.49427243695423 21.205915179435262 17.423130685960814 9 24.891167511668847 17.021250247192647 21.865955216238902 36.221627024899604 10-11 23.219080989018263 27.944969015296728 29.619957995632383 19.21599200005263 12-13 23.981459874296494 25.19387449850369 29.76999570823257 21.05466991896725 14-15 21.247257661230556 26.991056947380766 27.43705827012748 24.3246271212612 16-17 20.54859739699855 30.103875090605055 28.174089024956107 21.17343848744029 18-19 20.362549859385602 29.197340806968374 30.192283951692268 20.247825381953756 20-21 21.214227692774468 28.54466514732682 30.029446400700927 20.21166075919779 22-23 20.92940456772746 28.19344590194482 29.88111400917005 20.996035521157673 24-25 20.71342844745141 28.452511391006485 30.107106178837956 20.72695398270415 26-27 20.653818058679825 28.734934571753378 29.906808891468934 20.704438478097863 28-29 20.62995480959203 28.706836842687284 29.986233609902747 20.676974737817943 30-31 21.139943376983105 28.409163365463442 29.91876538950263 20.53212786805082 32-33 20.947983541754827 28.615840446488782 29.701221827003017 20.734954184753374 34-35 20.94127853774354 28.574266471580962 29.942814445799225 20.541640544876277 36-37 20.9935541446432 28.376034158079804 29.937933100256735 20.692478597020262 38-39 20.821824240061222 28.68383702446684 29.972165328467504 20.52217340700443 40-41 21.02054653635781 28.449937470091974 29.964151970412896 20.565364023137324 42-43 21.05551401849264 28.405448652046246 29.677508436775778 20.861528892685335 44-45 20.936757758210643 28.801477739664392 29.475317660618366 20.786446841506596 46-47 21.068691077814513 28.458267178187974 29.31055219279331 21.162489551204203 48-49 21.01913712311219 28.612563654221855 29.442505659685075 20.925793562980875 50-51 21.042402801167963 28.740022050716647 29.097544131722742 21.120031016392648 52-53 21.01650087913448 28.836657074787887 29.03108076733868 21.11576127873895 54-55 21.068830168625 28.76771793320317 29.074233182085212 21.08921871608662 56-57 21.071725507054296 28.883055824132615 28.896369376777702 21.14884929203539 58-59 20.875964916813437 28.837195710042234 29.319681937059162 20.967157436085163 60-61 21.03015500080157 28.98185159192696 29.05714586000187 20.9308475472696 62-63 20.982777746691593 28.874556056134733 29.06568599394501 21.07698020322866 64-65 20.797517881540756 28.740469579074855 29.186441296020867 21.275571243363522 66-67 20.901776365785185 28.76821212607643 28.93131440592934 21.39869710220905 68-69 20.891264781138954 28.745749764853784 28.98501795141818 21.377967502589083 70-71 20.898649005749107 28.332778156784766 29.206436944793147 21.56213589267298 72-73 21.049735711834764 28.552502047800854 29.079682976299388 21.318079264065 74-75 20.90583415153503 28.843273295662105 28.796916616371753 21.45397593643111 76-77 20.737554759523423 28.767720350945662 29.015700807991678 21.47902408153923 78-79 20.771582947508318 28.812894831254205 28.902044501688756 21.51347771954872 80-81 20.777315082933875 28.77973108917842 28.798393895704617 21.644559932183096 82-83 20.95479635002401 28.61548511484251 29.049315465029835 21.38040307010364 84-85 21.03866770226357 28.564256736701036 28.742341865397947 21.65473369563745 86-87 20.55240929142494 28.618145701053827 29.021655043291123 21.80778996423011 88-89 20.781373248740163 28.525862710342505 28.924825216264725 21.767938824652607 90-91 20.80667986012381 28.353074174204334 29.163074652172057 21.6771713134998 92-93 20.88127686549768 28.513828803403563 28.848109838874507 21.756784492224252 94-95 20.464765723225565 28.469094390389856 28.995616668174378 22.0705232182102 96-97 20.495145784174966 28.396255558327944 29.046648014181798 22.061950643315296 98-99 20.354452002444255 28.456246611380653 28.971490899634173 22.217810486540916 100-101 20.434782356308027 28.411306891556826 28.835220511320163 22.318690240814977 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 106.0 1 84.5 2 88.0 3 134.5 4 146.0 5 161.0 6 275.0 7 562.5 8 968.5 9 1209.5 10 1271.0 11 1333.5 12 1515.0 13 1818.5 14 2120.5 15 2483.0 16 2920.0 17 3422.5 18 3999.0 19 4707.0 20 5577.0 21 6671.0 22 8219.0 23 10961.5 24 14409.5 25 18483.5 26 25593.5 27 35022.0 28 43593.0 29 55651.5 30 70319.0 31 84918.0 32 105281.0 33 127875.0 34 149459.0 35 170606.0 36 197835.0 37 220558.0 38 224112.0 39 224821.0 40 227358.5 41 224014.0 42 219534.0 43 221795.5 44 225511.0 45 228312.5 46 223633.0 47 213994.5 48 208285.5 49 200849.0 50 186696.0 51 169019.0 52 151707.0 53 133986.0 54 116840.5 55 97340.5 56 77326.0 57 59020.0 58 44860.5 59 35307.5 60 26566.5 61 18090.0 62 11525.0 63 7115.0 64 4205.5 65 2382.0 66 1372.0 67 742.0 68 450.5 69 291.0 70 185.5 71 130.0 72 100.0 73 73.5 74 53.0 75 36.0 76 32.0 77 19.5 78 9.0 79 4.5 80 3.5 81 6.5 82 6.5 83 2.5 84 0.5 85 1.5 86 1.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0043149870918031885 2 0.006791750983062418 3 0.0021865181227522885 4 0.16900043614264149 5 0.0016060265857384065 6 4.2569379381018007E-4 7 0.0 8 8.126881518194346E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 2.9992062745717234E-4 24-25 6.09516113864576E-4 26-27 0.003076605146173574 28-29 2.4187147375578412E-4 30-31 0.006724026970410798 32-33 0.022068353265477743 34-35 0.01417366836208895 36-37 0.0011996825098286893 38-39 3.6764464010879185E-4 40-41 0.0020704198153495123 42-43 0.012141947982540362 44-45 0.007972083774990644 46-47 0.011077713498014913 48-49 0.010922915754811212 50-51 0.18327085309423274 52-53 0.11328292344825903 54-55 0.017753366173674553 56-57 0.007111021328420053 58-59 0.018256458839086585 60-61 0.12164200158125894 62-63 0.23447020665885712 64-65 0.24067179124595542 66-67 0.2512851115143592 68-69 0.246341258590791 70-71 0.2325158851509104 72-73 0.1592578531797585 74-75 0.2600021594285177 76-77 0.20064689976884825 78-79 0.006191909728148074 80-81 1.934971790046273E-5 82-83 0.0 84-85 0.0 86-87 0.0 88-89 8.707373055208227E-5 90-91 0.006211259446048536 92-93 0.018856300094000928 94-95 0.0031249794409247307 96-97 9.674858950231365E-6 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5168034.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.972528429059174 #Duplication Level Percentage of deduplicated Percentage of total 1 64.46444108998148 27.05735586312658 2 17.402285697608207 14.608358623469405 3 7.18012678538502 9.04104226871466 4 3.6932636646842423 6.200624566478826 5 2.058170872538774 4.319331772974762 6 1.3046228972407803 3.285499298618412 7 0.8495238686161137 2.495966530865792 8 0.5984455844558608 2.009461944545484 9 0.4228418620825498 1.5972967869530525 >10 1.7780716511983221 12.807829370540711 >50 0.13117055292606047 3.8465380222616696 >100 0.10735722922921892 8.869912008271937 >500 0.007409184157146528 2.1554364122696867 >1k 0.002176445206686832 1.4054240061952776 >5k 9.261468964576711E-5 0.2999225247137062 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8962 0.17341217182394697 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6469 0.1251733250980934 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 1.934971790046273E-5 1.934971790046273E-5 4 0.0 0.0 0.0 5.8049153701388184E-5 1.934971790046273E-5 5 0.0 0.0 0.0 5.8049153701388184E-5 1.934971790046273E-5 6 0.0 0.0 0.0 7.739887160185092E-5 1.934971790046273E-5 7 0.0 0.0 0.0 7.739887160185092E-5 3.869943580092546E-5 8 0.0 0.0 0.0 7.739887160185092E-5 3.869943580092546E-5 9 0.0 0.0 0.0 1.1609830740277637E-4 3.869943580092546E-5 10-11 0.0 0.0 0.0 1.1609830740277637E-4 3.869943580092546E-5 12-13 0.0 0.0 0.0 1.2577316635300773E-4 3.869943580092546E-5 14-15 0.0 0.0 0.0 1.5479774320370184E-4 3.869943580092546E-5 16-17 0.0 0.0 0.0 2.708960506064782E-4 3.869943580092546E-5 18-19 0.0 0.0 0.0 3.1927034535763504E-4 6.772401265161956E-5 20-21 0.0 0.0 0.0 3.482949222083291E-4 9.674858950231365E-5 22-23 0.0 0.0 0.0 4.1601893485994866E-4 9.674858950231365E-5 24-25 0.0 0.0 0.0 4.8374294751156823E-4 9.674858950231365E-5 26-27 0.0 0.0 0.0 6.482155496655014E-4 9.674858950231365E-5 28-29 0.0 0.0 0.0 0.0013254556761816971 9.674858950231365E-5 30-31 0.0 0.0 0.0 0.004518159129758047 9.674858950231365E-5 32-33 0.0 0.0 0.0 0.01290626183960864 9.674858950231365E-5 34-35 0.0 0.0 0.0 0.02582219853816751 9.674858950231365E-5 36-37 0.0 0.0 0.0 0.04600395430835014 9.674858950231365E-5 38-39 0.0 0.0 0.0 0.07982726119835898 9.674858950231365E-5 40-41 0.0 0.0 0.0 0.11813002778232495 9.674858950231365E-5 42-43 0.0 0.0 0.0 0.1605252597022388 9.674858950231365E-5 44-45 0.0 0.0 0.0 0.21245022768813054 1.1609830740277637E-4 46-47 0.0 0.0 0.0 0.27324704133138444 1.1609830740277637E-4 48-49 0.0 0.0 0.0 0.33781704996522854 1.1609830740277637E-4 50-51 0.0 0.0 0.0 0.40522179227149047 1.1609830740277637E-4 52-53 0.0 0.0 0.0 0.47777355953927547 1.5479774320370184E-4 54-55 0.0 0.0 0.0 0.5629993920318637 1.5479774320370184E-4 56-57 0.0 0.0 0.0 0.6635985753963694 1.8382232005439595E-4 58-59 0.0 0.0 0.0 0.7732340770203912 1.934971790046273E-4 60-61 0.0 0.0 0.0 0.8894194581537196 1.934971790046273E-4 62-63 0.0 0.0 0.0 1.0139058682663467 1.934971790046273E-4 64-65 0.0 0.0 0.0 1.1490152735063277 1.934971790046273E-4 66-67 0.0 0.0 0.0 1.309027765684204 1.934971790046273E-4 68-69 0.0 0.0 0.0 1.4860680096144878 2.0317203795485866E-4 70-71 0.0 0.0 0.0 1.6798264098107714 2.3219661480555274E-4 72-73 0.0 0.0 0.0 1.885968629463351 2.3219661480555274E-4 74-75 0.0 0.0 9.674858950231365E-6 2.1036045815488054 2.4187147375578412E-4 76-77 0.0 0.0 1.934971790046273E-5 2.3513583695463307 2.5154633270601547E-4 78-79 0.0 0.0 1.934971790046273E-5 2.6285140538936083 2.7089605060647817E-4 80-81 0.0 0.0 1.934971790046273E-5 2.9320917780339677 3.1927034535763504E-4 82-83 0.0 0.0 1.934971790046273E-5 3.2565575226478773 3.4829492220832915E-4 84-85 0.0 0.0 1.934971790046273E-5 3.588608356678768 3.4829492220832915E-4 86-87 0.0 0.0 1.934971790046273E-5 3.9560885241854056 3.4829492220832915E-4 88-89 0.0 0.0 1.934971790046273E-5 4.36271317100468 3.579697811585605E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5320 0.0 53.780533 1 GTATCAA 11115 0.0 45.53867 1 ATCAACG 13850 0.0 35.6157 3 CAACGCA 13890 0.0 35.554092 5 TCAACGC 14345 0.0 34.4851 4 TATCAAC 14775 0.0 33.70955 2 AACGCAG 15055 0.0 33.21149 6 ACGCAGA 17080 0.0 28.968428 7 CGCAGAG 17065 0.0 28.910498 8 GCAGAGT 19865 0.0 24.405676 9 ACATGGG 15095 0.0 23.76883 3 TACATGG 15790 0.0 23.295822 2 GTACATG 16525 0.0 23.123568 1 AGAGTAC 18740 0.0 20.871305 10-11 GAGTACT 13050 0.0 19.81137 12-13 CATGGGG 8375 0.0 19.689104 4 CAGAGTA 19975 0.0 18.809053 10-11 GAGTACA 11115 0.0 18.685822 1 GGCGTGC 1690 0.0 18.526175 8 GCGTGCG 2035 0.0 17.949602 9 >>END_MODULE