FastQCFastQC Report
Sat 18 Jun 2016
SRR1931008_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931008_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2920141
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1281694.389137373845989No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20450.057.0968481
GTATCAA39000.045.8280531
ATCAACG46100.038.6659973
TCAACGC47050.037.1351854
CAACGCA48750.036.033885
TATCAAC50350.035.084162
AACGCAG54250.033.312526
ACGCAGA61950.028.6352527
CGCAGAG61550.028.1438488
TACATGG43200.025.6012672
GTACATG44100.025.3156171
ACATGGG44150.024.406753
GCAGAGT68050.023.6162789
GAGTACT49050.020.85349812-13
CAGAGTA66950.020.1268610-11
AGAGTAC60950.019.8898410-11
GTGTAGC12750.019.279761
GTACTTT57600.017.59202414-15
AGTACTT53450.017.429812-13
GAGTACA29850.017.3279671
Produced by FastQC (version 0.11.5)