##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931008_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2920141 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 20.395997316567932 28.0 2.0 31.0 2.0 33.0 2 19.198811290276737 26.0 2.0 31.0 2.0 33.0 3 19.77382907195235 26.0 2.0 31.0 2.0 33.0 4 21.324381939091296 28.0 2.0 35.0 2.0 37.0 5 20.712639218448697 28.0 2.0 35.0 2.0 37.0 6 21.569601262404795 28.0 2.0 35.0 2.0 37.0 7 21.693371655683748 28.0 2.0 35.0 2.0 37.0 8 22.0318498319088 30.0 2.0 35.0 2.0 37.0 9 23.194526223220045 30.0 2.0 37.0 2.0 39.0 10-11 23.451589666389395 32.0 2.0 37.0 2.0 39.0 12-13 23.30331549058761 32.0 2.0 37.0 2.0 39.0 14-15 23.2364022833144 31.0 2.0 38.0 2.0 40.0 16-17 22.19120891765158 29.0 2.0 37.0 2.0 39.5 18-19 21.242449422818968 27.0 2.0 37.0 2.0 39.0 20-21 20.9201978603088 27.0 2.0 37.0 2.0 39.0 22-23 20.558180409781585 25.0 2.0 36.0 2.0 39.0 24-25 20.369582153738467 25.0 2.0 36.0 2.0 39.0 26-27 20.06589544819925 24.5 2.0 36.0 2.0 39.0 28-29 19.835376784888126 24.0 2.0 36.0 2.0 39.0 30-31 19.75191677388181 24.0 2.0 36.0 2.0 39.0 32-33 19.592303762044367 24.0 2.0 36.0 2.0 39.0 34-35 19.232313782108466 22.5 2.0 35.0 2.0 39.0 36-37 18.80129230061151 19.0 2.0 35.0 2.0 38.0 38-39 18.612823147923336 18.0 2.0 35.0 2.0 38.0 40-41 18.424591141318174 18.0 2.0 34.5 2.0 38.0 42-43 18.25056135987954 17.0 2.0 34.0 2.0 38.0 44-45 17.84722039107016 15.0 2.0 34.0 2.0 38.0 46-47 17.514286296449384 15.0 2.0 33.0 2.0 38.0 48-49 17.215197485326907 11.0 2.0 33.0 2.0 38.0 50-51 15.214881404699295 7.0 2.0 31.0 2.0 37.5 52-53 16.99733557386441 12.0 2.0 33.0 2.0 37.5 54-55 17.645510781842383 12.5 2.0 34.5 2.0 38.0 56-57 17.567012859995458 9.5 2.0 34.5 2.0 38.0 58-59 17.277015904369 8.0 2.0 34.0 2.0 38.0 60-61 16.975882500194338 7.5 2.0 34.0 2.0 38.0 62-63 16.528350685805925 4.5 2.0 33.5 2.0 38.0 64-65 16.148033776451207 2.0 2.0 33.0 2.0 38.0 66-67 15.762863334338991 2.0 2.0 32.5 2.0 37.5 68-69 15.308067144702944 2.0 2.0 31.5 2.0 37.0 70-71 14.93650546326359 2.0 2.0 31.0 2.0 36.0 72-73 14.520664755571733 2.0 2.0 31.0 2.0 36.0 74-75 14.053847742283677 2.0 2.0 30.5 2.0 35.5 76-77 13.612599699809016 2.0 2.0 30.0 2.0 35.0 78-79 13.203738620840568 2.0 2.0 29.0 2.0 34.0 80-81 12.752656121742067 2.0 2.0 29.0 2.0 34.0 82-83 12.276541098529147 2.0 2.0 28.5 2.0 34.0 84-85 11.853594398352682 2.0 2.0 27.5 2.0 34.0 86-87 11.36066460489408 2.0 2.0 26.0 2.0 34.0 88-89 10.92511525984533 2.0 2.0 26.0 2.0 33.0 90-91 10.431791478562165 2.0 2.0 25.0 2.0 32.5 92-93 9.897931127298305 2.0 2.0 22.5 2.0 32.0 94-95 9.339378646442071 2.0 2.0 19.0 2.0 32.0 96-97 8.729345432292483 2.0 2.0 6.0 2.0 32.0 98-99 8.096174123098852 2.0 2.0 2.0 2.0 31.0 100-101 7.293060163875648 2.0 2.0 2.0 2.0 30.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 8.25230184009088 1101 2 9.508313180973875 1101 3 9.04790892869589 1101 4 11.03632892178662 1101 5 11.73817342903937 1101 6 11.163473438218801 1101 7 10.575288216710504 1101 8 10.201653175969817 1101 9 10.252049792720392 1101 10-11 9.845271416944012 1101 12-13 9.82378498428221 1101 14-15 10.387647907853331 1101 16-17 11.122963744202007 1101 18-19 11.994864059172553 1101 20-21 11.99284468828564 1101 22-23 12.201527623911986 1101 24-25 12.030773668884386 1101 26-27 12.258824075567698 1101 28-29 12.11144466712247 1101 30-31 11.736018263118726 1101 32-33 11.61142692752874 1101 34-35 11.364538429932555 1101 36-37 11.60211540939628 1101 38-39 11.449352669327698 1101 40-41 11.025929581097085 1101 42-43 11.006166111297691 1101 44-45 10.967847489807408 1101 46-47 10.505862143224725 1101 48-49 10.659778151062117 1101 50-51 12.260558042574289 1101 52-53 11.587150308332145 1101 54-55 11.3131349673661 1101 56-57 11.384309106196898 1101 58-59 11.216369773062205 1101 60-61 10.947935521666476 1101 62-63 10.891752003400196 1101 64-65 10.803794022006203 1101 66-67 10.488931147403468 1101 68-69 10.548050731008077 1101 70-71 10.448269476676963 1101 72-73 10.100270365547084 1101 74-75 9.833041068613083 1101 76-77 9.57740654175361 1101 78-79 9.061639239192507 1101 80-81 8.896763905395149 1101 82-83 8.51528224821613 1101 84-85 8.2884238221527 1101 86-87 8.115484106254934 1101 88-89 7.814911335553209 1101 90-91 7.431480444405844 1101 92-93 6.977737791434883 1101 94-95 6.525594743488581 1101 96-97 6.433966513138092 1101 98-99 6.168809935197543 1101 100-101 5.467372257941276 1102 1 7.844373296326097 1102 2 9.148210202917657 1102 3 8.477077532199175 1102 4 10.587407258824268 1102 5 11.150680186612199 1102 6 10.31542200356325 1102 7 10.10111696365929 1102 8 9.624906535592132 1102 9 9.5208387420777 1102 10-11 8.827096275809346 1102 12-13 8.984087313522299 1102 14-15 9.340831465592299 1102 16-17 9.973316764708926 1102 18-19 10.98048151525892 1102 20-21 10.992251938286639 1102 22-23 11.177517572119896 1102 24-25 10.942508625847616 1102 26-27 10.999175732303947 1102 28-29 10.875715011929152 1102 30-31 11.040956940234434 1102 32-33 10.662740916116743 1102 34-35 10.394408016019245 1102 36-37 10.697411336490706 1102 38-39 10.492536957976451 1102 40-41 10.251445329305799 1102 42-43 9.927451585491006 1102 44-45 9.7963347205017 1102 46-47 9.811412734437141 1102 48-49 9.50522070578787 1102 50-51 11.314673666260937 1102 52-53 10.825081383540535 1102 54-55 10.312277373993492 1102 56-57 10.258414243111005 1102 58-59 10.327735687441471 1102 60-61 10.309423105782383 1102 62-63 10.063523488025016 1102 64-65 9.76483029162099 1102 66-67 9.721297614867511 1102 68-69 9.578899468784838 1102 70-71 9.06024942211722 1102 72-73 8.923779276184002 1102 74-75 8.716096619092871 1102 76-77 8.33257059757194 1102 78-79 7.962053785366619 1102 80-81 7.801192104429008 1102 82-83 7.720203276534873 1102 84-85 7.559063555388461 1102 86-87 7.269579161962296 1102 88-89 6.948086104547841 1102 90-91 6.494079746743553 1102 92-93 6.257584425171988 1102 94-95 5.979191332100918 1102 96-97 5.615039270303571 1102 98-99 5.058203676738043 1102 100-101 4.57708493463537 1103 1 7.937447192217707 1103 2 9.224815005476163 1103 3 8.47449152574756 1103 4 10.696141671581579 1103 5 11.177509867350341 1103 6 10.46743187340434 1103 7 10.137627879387122 1103 8 9.672557850310668 1103 9 9.705565614077685 1103 10-11 9.210603516823529 1103 12-13 9.010459425620784 1103 14-15 9.49580177565812 1103 16-17 10.249300550903914 1103 18-19 11.204428595285652 1103 20-21 11.36197769564745 1103 22-23 11.447931921524734 1103 24-25 11.370841086428289 1103 26-27 11.31990977504028 1103 28-29 10.938250899932648 1103 30-31 10.972741404539722 1103 32-33 11.060283948488784 1103 34-35 10.957913084413196 1103 36-37 11.044919491443636 1103 38-39 10.613449103272227 1103 40-41 10.572948560233975 1103 42-43 10.086066427864903 1103 44-45 10.098458865372972 1103 46-47 9.935711236331592 1103 48-49 10.012697232445834 1103 50-51 11.46763304959417 1103 52-53 10.823264837675474 1103 54-55 10.521688818363188 1103 56-57 10.381425952379114 1103 58-59 10.089732495411265 1103 60-61 9.97107111776949 1103 62-63 9.802592724005788 1103 64-65 9.445614086706945 1103 66-67 9.076954239757546 1103 68-69 8.935729788016188 1103 70-71 8.896557959468744 1103 72-73 8.7099189179174 1103 74-75 8.566530729099805 1103 76-77 8.289248343076395 1103 78-79 7.708162735842864 1103 80-81 7.661296172284496 1103 82-83 7.27726762640823 1103 84-85 7.14729040526999 1103 86-87 6.7267163283904505 1103 88-89 6.410084535990119 1103 90-91 6.1455122487500145 1103 92-93 5.92490048083552 1103 94-95 5.5798264149274335 1103 96-97 5.185892839840166 1103 98-99 4.896024277143932 1103 100-101 4.363241955151488 1104 1 8.02295674890643 1104 2 9.269611563723789 1104 3 8.845548411332981 1104 4 10.47920819868314 1104 5 11.277215692272957 1104 6 10.579820517414085 1104 7 10.45720604705091 1104 8 9.67976978120673 1104 9 9.633862439611697 1104 10-11 9.184140083842 1104 12-13 9.027297827792367 1104 14-15 9.43810824911034 1104 16-17 10.335071952220826 1104 18-19 10.960038408377244 1104 20-21 11.00405065362747 1104 22-23 11.404779254582778 1104 24-25 11.227412490306023 1104 26-27 11.388973536447967 1104 28-29 11.12273148405744 1104 30-31 10.954574412968046 1104 32-33 11.012711933213449 1104 34-35 10.673307499888278 1104 36-37 10.576491455502417 1104 38-39 10.114820746587345 1104 40-41 9.969667019640056 1104 42-43 9.712381973049109 1104 44-45 9.783339898923018 1104 46-47 9.811428027998659 1104 48-49 9.864364651355626 1104 50-51 11.139465705908691 1104 52-53 10.747082615581295 1104 54-55 10.40022348076608 1104 56-57 9.991888533885461 1104 58-59 10.171111757188669 1104 60-61 9.862049962131987 1104 62-63 9.71277025338328 1104 64-65 9.438723219578689 1104 66-67 9.314419148416027 1104 68-69 8.947787445050944 1104 70-71 8.821115016309516 1104 72-73 8.54745649006072 1104 74-75 8.362488942794469 1104 76-77 8.144481394398582 1104 78-79 7.889779194282232 1104 80-81 7.587439021653411 1104 82-83 7.349323714936046 1104 84-85 7.124499999410814 1104 86-87 6.877545396345214 1104 88-89 6.395841052496255 1104 90-91 6.042131796187748 1104 92-93 5.57402243705177 1104 94-95 5.3118532459992345 1104 96-97 4.952072469096343 1104 98-99 4.568722973362552 1104 100-101 3.9264382201843455 1105 1 8.09506443217916 1105 2 9.137595844814864 1105 3 8.55447432754045 1105 4 10.705944713283731 1105 5 10.444516488214528 1105 6 10.524686881697924 1105 7 9.991560234803156 1105 8 9.69271164123597 1105 9 9.803596667598349 1105 10-11 9.318503330583884 1105 12-13 8.88243271302434 1105 14-15 9.677729146012783 1105 16-17 10.445362339695674 1105 18-19 10.723011506301798 1105 20-21 11.282126649179194 1105 22-23 11.535794423704743 1105 24-25 11.49519997265661 1105 26-27 11.585610777017248 1105 28-29 11.27535421774813 1105 30-31 10.932498904727488 1105 32-33 10.74949111877904 1105 34-35 10.883380783990692 1105 36-37 10.857016189667931 1105 38-39 10.758168324400941 1105 40-41 10.526692734675478 1105 42-43 10.05405863584227 1105 44-45 10.011773568376103 1105 46-47 10.029020898261429 1105 48-49 9.656694186160887 1105 50-51 10.921192161906994 1105 52-53 10.216086813954423 1105 54-55 9.986292941350104 1105 56-57 9.888389889610114 1105 58-59 9.856701403700644 1105 60-61 9.684783028068939 1105 62-63 9.689026953911828 1105 64-65 9.409250890106359 1105 66-67 9.233429281989265 1105 68-69 8.881751159111742 1105 70-71 8.936074531997189 1105 72-73 8.68971261581199 1105 74-75 8.230670335004993 1105 76-77 7.934565236909606 1105 78-79 7.703080290107598 1105 80-81 7.450601108844625 1105 82-83 7.345467396759338 1105 84-85 7.062076376064864 1105 86-87 6.710756589507865 1105 88-89 6.324087294577449 1105 90-91 6.078018515084173 1105 92-93 5.808903920476942 1105 94-95 5.202947946802674 1105 96-97 4.89402933581049 1105 98-99 4.508103026092118 1105 100-101 3.898489050487604 1106 1 7.746996990022261 1106 2 8.921400804498418 1106 3 8.533685967295224 1106 4 10.166107089260805 1106 5 10.793488943097525 1106 6 10.079994220072354 1106 7 9.432870097838258 1106 8 9.066793779056127 1106 9 8.728632748864463 1106 10-11 8.529330691491115 1106 12-13 8.412572391726318 1106 14-15 8.845308637873426 1106 16-17 9.810245913365193 1106 18-19 10.776080572629649 1106 20-21 10.805229350237166 1106 22-23 10.947569560320137 1106 24-25 10.691334034605369 1106 26-27 10.801858551491858 1106 28-29 10.612881612783518 1106 30-31 10.552259411468825 1106 32-33 10.72569362205822 1106 34-35 10.547398921251638 1106 36-37 10.684457997061816 1106 38-39 10.351667939945283 1106 40-41 10.198908001060076 1106 42-43 9.88654318565909 1106 44-45 9.406678091795005 1106 46-47 9.486137748109911 1106 48-49 9.514638129647963 1106 50-51 10.898255784178282 1106 52-53 10.613511475037232 1106 54-55 9.991103057262457 1106 56-57 9.932482224756765 1106 58-59 9.57317964597761 1106 60-61 9.436760199215438 1106 62-63 9.444883015358943 1106 64-65 9.26763695759786 1106 66-67 9.065294899318141 1106 68-69 8.686576385981697 1106 70-71 8.054976695228426 1106 72-73 7.855892442375202 1106 74-75 7.425345007395316 1106 76-77 7.237101616283049 1106 78-79 7.161603692560716 1106 80-81 6.8399116996727845 1106 82-83 6.566959187500329 1106 84-85 6.24580804246426 1106 86-87 6.035653588286651 1106 88-89 5.726433399239848 1106 90-91 5.427815802101907 1106 92-93 5.209334222983921 1106 94-95 4.96721166282358 1106 96-97 4.336969126354115 1106 98-99 3.923102244845329 1106 100-101 3.4850530448790744 1107 1 7.160886088460295 1107 2 8.077464261868986 1107 3 7.986281533597818 1107 4 9.707540323845521 1107 5 10.694870064509395 1107 6 9.661121748509306 1107 7 9.573067989252838 1107 8 8.116056834364187 1107 9 5.5867004022017674 1107 10-11 7.766843103030229 1107 12-13 7.737508524545589 1107 14-15 8.160377214812737 1107 16-17 9.020306141187021 1107 18-19 10.321488048300996 1107 20-21 10.586806956252609 1107 22-23 9.83425501165161 1107 24-25 9.9391488901218 1107 26-27 10.111648426938114 1107 28-29 10.195206561659191 1107 30-31 10.007774620319093 1107 32-33 10.431009940454214 1107 34-35 10.552374750607502 1107 36-37 10.556430448032412 1107 38-39 10.009241578345925 1107 40-41 9.699270305865046 1107 42-43 9.499040254802832 1107 44-45 9.090504352674852 1107 46-47 9.287377474821582 1107 48-49 9.32658277061552 1107 50-51 10.866911219463958 1107 52-53 9.919710108595588 1107 54-55 9.73618339840393 1107 56-57 9.50144950314835 1107 58-59 9.400313971962223 1107 60-61 9.203012740140384 1107 62-63 9.210254056664137 1107 64-65 9.067855319131978 1107 66-67 9.196013025364328 1107 68-69 8.866198524701419 1107 70-71 8.627555909901666 1107 72-73 8.191855017124782 1107 74-75 8.052617669795337 1107 76-77 7.8717122252691 1107 78-79 7.6111071290533925 1107 80-81 7.283055942655553 1107 82-83 6.957609908949712 1107 84-85 6.932226097762127 1107 86-87 6.534857618698238 1107 88-89 6.454561361953239 1107 90-91 6.293666586348069 1107 92-93 5.690496106153365 1107 94-95 5.439644895369437 1107 96-97 4.91210730985528 1107 98-99 4.597353423814117 1107 100-101 4.3093867975372335 1108 1 6.812941408394817 1108 2 7.883136331695955 1108 3 7.813575795910683 1108 4 8.988571723139735 1108 5 10.684192822663153 1108 6 9.179814289872155 1108 7 8.538662087870371 1108 8 5.989766439092563 1108 9 6.866685322091751 1108 10-11 6.0624956391207725 1108 12-13 5.023044001486813 1108 14-15 7.016323788488119 1108 16-17 8.272245187906297 1108 18-19 7.741468293520615 1108 20-21 7.875982188031525 1108 22-23 8.620399382828626 1108 24-25 8.259501041800526 1108 26-27 6.804057265749176 1108 28-29 8.718391821465662 1108 30-31 9.162184365410383 1108 32-33 9.758604454143178 1108 34-35 9.329861157427448 1108 36-37 9.007811113153682 1108 38-39 8.746951022612759 1108 40-41 8.985980999848927 1108 42-43 7.986223286977417 1108 44-45 7.597398263445957 1108 46-47 8.328422574747776 1108 48-49 8.760980812657266 1108 50-51 10.145754692915965 1108 52-53 9.630067102774355 1108 54-55 8.943558038849325 1108 56-57 8.846552028657438 1108 58-59 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-12.579208608285953 2313 72-73 -12.167430241330868 2313 74-75 -11.731524002189348 2313 76-77 -11.295486243141873 2313 78-79 -10.902453815426506 2313 80-81 -10.456573923330408 2313 82-83 -9.983200556908615 2313 84-85 -9.570645631657145 2313 86-87 -9.08798858423731 2313 88-89 -8.66030781481766 2313 90-91 -8.178094741029083 2313 92-93 -7.675330313757293 2313 94-95 -7.13135063749591 2313 96-97 -6.542264100482946 2313 98-99 -5.926099033109941 2313 100-101 -5.1494517474531945 2314 1 -17.970894383779655 2314 2 -16.802880345661325 2314 3 -17.35768783462171 2314 4 -18.870953613614596 2314 5 -18.22440243070439 2314 6 -19.106587249576208 2314 7 -19.222081978839057 2314 8 -19.58560238707806 2314 9 -20.725360861359178 2314 10-11 -21.004694867614315 2314 12-13 -20.874967544375917 2314 14-15 -20.79846133017769 2314 16-17 -19.73256558823225 2314 18-19 -18.772162238871946 2314 20-21 -18.453716336663792 2314 22-23 -18.084379321469 2314 24-25 -17.89572666827003 2314 26-27 -17.602774036367567 2314 28-29 -17.385520943127027 2314 30-31 -17.30157445434587 2314 32-33 -17.158970914682993 2314 34-35 -16.804038778428872 2314 36-37 -16.375126667474927 2314 38-39 -16.189386373153756 2314 40-41 -16.01722618424344 2314 42-43 -15.847003140219503 2314 44-45 -15.440446508843973 2314 46-47 -15.117536373295842 2314 48-49 -14.82673567226897 2314 50-51 -12.91021066949584 2314 52-53 -14.604859131991521 2314 54-55 -15.238449658392497 2314 56-57 -15.15665515543493 2314 58-59 -14.867075945076703 2314 60-61 -14.579373986088076 2314 62-63 -14.136906122021248 2314 64-65 -13.76127678042131 2314 66-67 -13.39205334349336 2314 68-69 -12.954477927117344 2314 70-71 -12.579208608285953 2314 72-73 -12.167430241330868 2314 74-75 -11.731524002189348 2314 76-77 -11.295486243141873 2314 78-79 -10.902453815426506 2314 80-81 -10.456573923330408 2314 82-83 -9.983200556908615 2314 84-85 -9.570645631657145 2314 86-87 -9.08798858423731 2314 88-89 -8.66030781481766 2314 90-91 -8.178094741029083 2314 92-93 -7.675330313757293 2314 94-95 -7.13135063749591 2314 96-97 -6.542264100482946 2314 98-99 -5.926099033109941 2314 100-101 -5.1494517474531945 2315 1 -17.970894383779655 2315 2 -16.802880345661325 2315 3 -17.35768783462171 2315 4 -18.870953613614596 2315 5 -18.22440243070439 2315 6 -19.106587249576208 2315 7 -19.222081978839057 2315 8 -19.58560238707806 2315 9 -20.725360861359178 2315 10-11 -21.004694867614315 2315 12-13 -20.874967544375917 2315 14-15 -20.79846133017769 2315 16-17 -19.73256558823225 2315 18-19 -18.772162238871946 2315 20-21 -18.453716336663792 2315 22-23 -18.084379321469 2315 24-25 -17.89572666827003 2315 26-27 -17.602774036367567 2315 28-29 -17.385520943127027 2315 30-31 -17.30157445434587 2315 32-33 -17.158970914682993 2315 34-35 -16.804038778428872 2315 36-37 -16.375126667474927 2315 38-39 -16.189386373153756 2315 40-41 -16.01722618424344 2315 42-43 -15.847003140219503 2315 44-45 -15.440446508843973 2315 46-47 -15.117536373295842 2315 48-49 -14.82673567226897 2315 50-51 -12.91021066949584 2315 52-53 -14.604859131991521 2315 54-55 -15.238449658392497 2315 56-57 -15.15665515543493 2315 58-59 -14.867075945076703 2315 60-61 -14.579373986088076 2315 62-63 -14.136906122021248 2315 64-65 -13.76127678042131 2315 66-67 -13.39205334349336 2315 68-69 -12.954477927117344 2315 70-71 -12.579208608285953 2315 72-73 -12.167430241330868 2315 74-75 -11.731524002189348 2315 76-77 -11.295486243141873 2315 78-79 -10.902453815426506 2315 80-81 -10.456573923330408 2315 82-83 -9.983200556908615 2315 84-85 -9.570645631657145 2315 86-87 -9.08798858423731 2315 88-89 -8.66030781481766 2315 90-91 -8.178094741029083 2315 92-93 -7.675330313757293 2315 94-95 -7.13135063749591 2315 96-97 -6.542264100482946 2315 98-99 -5.926099033109941 2315 100-101 -5.1494517474531945 2316 1 -17.970894383779655 2316 2 -16.802880345661325 2316 3 -17.35768783462171 2316 4 -18.870953613614596 2316 5 -18.22440243070439 2316 6 -19.106587249576208 2316 7 -19.222081978839057 2316 8 -19.58560238707806 2316 9 -20.725360861359178 2316 10-11 -21.004694867614315 2316 12-13 -20.874967544375917 2316 14-15 -20.79846133017769 2316 16-17 -19.73256558823225 2316 18-19 -18.772162238871946 2316 20-21 -18.453716336663792 2316 22-23 -18.084379321469 2316 24-25 -17.89572666827003 2316 26-27 -17.602774036367567 2316 28-29 -17.385520943127027 2316 30-31 -17.30157445434587 2316 32-33 -17.158970914682993 2316 34-35 -16.804038778428872 2316 36-37 -16.375126667474927 2316 38-39 -16.189386373153756 2316 40-41 -16.01722618424344 2316 42-43 -15.847003140219503 2316 44-45 -15.440446508843973 2316 46-47 -15.117536373295842 2316 48-49 -14.82673567226897 2316 50-51 -12.91021066949584 2316 52-53 -14.604859131991521 2316 54-55 -15.238449658392497 2316 56-57 -15.15665515543493 2316 58-59 -14.867075945076703 2316 60-61 -14.579373986088076 2316 62-63 -14.136906122021248 2316 64-65 -13.76127678042131 2316 66-67 -13.39205334349336 2316 68-69 -12.954477927117344 2316 70-71 -12.579208608285953 2316 72-73 -12.167430241330868 2316 74-75 -11.731524002189348 2316 76-77 -11.295486243141873 2316 78-79 -10.902453815426506 2316 80-81 -10.456573923330408 2316 82-83 -9.983200556908615 2316 84-85 -9.570645631657145 2316 86-87 -9.08798858423731 2316 88-89 -8.66030781481766 2316 90-91 -8.178094741029083 2316 92-93 -7.675330313757293 2316 94-95 -7.13135063749591 2316 96-97 -6.542264100482946 2316 98-99 -5.926099033109941 2316 100-101 -5.1494517474531945 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 799667.0 3 35956.0 4 107983.0 5 63001.0 6 41588.0 7 40347.0 8 39178.0 9 40034.0 10 42654.0 11 44349.0 12 44381.0 13 45144.0 14 45859.0 15 46718.0 16 47293.0 17 47617.0 18 47553.0 19 48763.0 20 50500.0 21 53600.0 22 55841.0 23 58826.0 24 62308.0 25 65577.0 26 69729.0 27 73670.0 28 78532.0 29 83108.0 30 88431.0 31 94200.0 32 99077.0 33 102472.0 34 98526.0 35 84399.0 36 53627.0 37 17985.0 38 1639.0 39 9.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.01008685045882 20.583449053507692 14.170906083480864 23.235558012552623 2 14.682220240534091 20.84934082090092 42.32503434479767 22.143404593767325 3 18.688248775174987 26.237374812418185 30.228477314573375 24.845899097833453 4 11.96122213987935 17.648223204435894 40.30975940056535 30.08079525511941 5 12.466738002463105 38.15544810320809 36.10405604570875 13.273757848620058 6 29.069710233132767 39.23769921244347 18.48284212789685 13.209748426526916 7 25.888482167035594 33.14525628641242 23.260253634561987 17.706007911990003 8 23.955188287502395 37.23964838254176 21.024032259923985 17.781131070031858 9 25.067763128560934 17.29238273033745 21.672530876762412 35.96732326433921 10-11 23.49092523431369 27.658658295620363 29.57962420050726 19.27079226955869 12-13 24.295270285858376 24.99001294775284 29.649200489416156 21.06551627697263 14-15 21.457920702559072 26.78948969584281 27.4959206727752 24.25666892882292 16-17 20.92552547357495 29.78399969836697 28.150435039112487 21.140039788945593 18-19 20.61163464261381 28.821343823278443 30.29359509596935 20.27342643813839 20-21 21.494565955532927 28.12799205380222 30.200311203643675 20.177130787021177 22-23 21.036052672784013 28.098398708161366 29.96477564343915 20.90077297561547 24-25 20.87685191455202 28.235257730364477 30.268641104940635 20.61924925014287 26-27 20.835895972844863 28.435693159938815 29.95363593745296 20.774774929763367 28-29 20.81858621178342 28.5378071326459 30.082935503118968 20.560671152451718 30-31 21.153636765343254 28.255801730306995 30.026439753084738 20.56412175126502 32-33 20.876023383944727 28.434954733680662 29.957982481759277 20.731039400615337 34-35 21.129956481002495 28.402227861564004 29.98978497346535 20.47803068396815 36-37 21.127569929461313 28.30761247660707 29.86528305330813 20.69953454062349 38-39 21.104012359575194 28.48543579925308 29.875581565016596 20.534970276155132 40-41 21.40834656955973 28.425388909476894 29.710201392997682 20.45606312796569 42-43 21.17201051573466 28.324904679391967 29.622373700767056 20.88071110410632 44-45 21.267870122621325 28.67389268815252 29.455449057044063 20.60278813218209 46-47 21.305215945632085 28.51543628583161 29.076434882158527 21.10291288637778 48-49 21.26232916291211 28.608395897031702 29.22547949512147 20.90379544493472 50-51 21.446311880591256 28.81044633121893 28.861298996722272 20.881942791467544 52-53 20.97439367062019 29.052645320064336 28.934698811489064 21.03826219782641 54-55 20.935253637807634 28.9958861733854 28.991617842069516 21.07724234673745 56-57 21.01893505512859 29.081554296245443 29.009152598462194 20.89035805016377 58-59 20.960702742763026 29.098831010611498 29.05733847451435 20.88312777211113 60-61 20.971697279149534 29.192828834576513 29.024579596242322 20.81089429003163 62-63 20.940000377118935 29.08956305383732 29.001052341414752 20.96938422762899 64-65 20.750065959080263 28.944870541815853 29.137396177764895 21.167667321338985 66-67 20.81985990367186 29.051644199928223 28.762265189904145 21.366230706495777 68-69 20.9216445192572 28.97346442731416 28.892511259339372 21.212379794089273 70-71 21.045621786393525 28.73563547502183 28.922100361983276 21.296642376601365 72-73 21.043588670392555 28.82438440191758 28.90222445369077 21.229802473999094 74-75 20.928251564358455 29.12275816033359 28.582580163073807 21.36641011223415 76-77 20.909084748089676 28.99473506671182 28.71287323519362 21.383306950004886 78-79 20.847470670429704 29.088241194211562 28.593931580476468 21.470356554882265 80-81 20.94450936306618 29.028779696618912 28.357687855447704 21.669023084867195 82-83 21.112600417478113 28.864063672053852 28.57032788061224 21.4530080298558 84-85 21.148048677332103 28.86647185222511 28.428000865052827 21.55747860538996 86-87 20.999862047540375 28.878511677621393 28.5047582736173 21.616868001220936 88-89 21.143486366130684 28.89292214658853 28.389262132941496 21.574329354339294 90-91 21.065649049252045 28.941289448727105 28.421412443647775 21.571649058373072 92-93 21.037739945205004 28.92709052463387 28.338760729963138 21.696408800197993 94-95 20.93259082384987 28.808047362879474 28.361713260224136 21.89764855304652 96-97 20.96278146746752 28.782409440901752 28.349609716084988 21.905199375545735 98-99 20.852017324821496 28.843138511380577 28.09995283261672 22.204891331181212 100-101 21.16753689413411 28.9631849059809 27.997694646143838 21.871583553741146 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 172830.0 1 157109.5 2 104669.5 3 53109.5 4 32988.0 5 25980.5 6 24142.0 7 24410.5 8 24902.0 9 24519.0 10 22432.0 11 18899.5 12 15022.5 13 11395.5 14 8494.0 15 6794.0 16 6330.5 17 6738.5 18 7749.5 19 9216.5 20 11005.5 21 12988.5 22 15070.5 23 17362.0 24 19898.5 25 22941.0 26 26457.5 27 30410.5 28 34636.0 29 39101.0 30 43782.5 31 49143.5 32 55231.5 33 61039.5 34 66933.0 35 72424.5 36 78033.0 37 83161.5 38 85978.5 39 87221.0 40 88464.0 41 89241.5 42 89225.5 43 89120.5 44 88575.5 45 87478.0 46 85499.5 47 82640.5 48 79324.0 49 75630.0 50 70720.5 51 65135.5 52 59066.5 53 52583.0 54 46542.0 55 39842.0 56 33143.0 57 27126.5 58 21847.0 59 17628.0 60 13725.0 61 10340.0 62 7506.5 63 5184.5 64 3502.5 65 2380.0 66 1609.5 67 1025.5 68 663.5 69 423.0 70 259.5 71 166.5 72 110.0 73 78.5 74 57.0 75 38.0 76 27.0 77 20.5 78 14.5 79 8.5 80 6.5 81 5.5 82 4.0 83 4.5 84 4.0 85 2.0 86 0.0 87 0.0 88 1.0 89 1.0 90 0.0 91 0.0 92 1.0 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 21.948803157107825 2 28.95719761477271 3 22.777153568954375 4 29.0690072842373 5 32.1525912618603 6 24.82746552306892 7 23.7452232614795 8 19.799180929961942 9 11.675326636624739 10-11 15.302480256946497 12-13 15.39110611439653 14-15 19.51535901862273 16-17 22.798419665351776 18-19 26.717442753620457 20-21 28.77095660791722 22-23 28.373578536104933 24-25 26.92453891781253 26-27 28.34013837003076 28-29 25.33343424170271 30-31 27.190005551101816 32-33 26.1881361208243 34-35 24.2691363191024 36-37 27.207556073490974 38-39 27.36280542617634 40-41 24.410293886493836 42-43 25.255869493973066 44-45 23.983071365389545 46-47 27.236989583722153 48-49 22.66246732606405 50-51 28.281356961872735 52-53 21.825196112105548 54-55 22.978976015199265 56-57 25.079165697820756 58-59 24.358755279282747 60-61 23.887391054062114 62-63 23.72238189868229 64-65 22.252144673835954 66-67 22.75991125086083 68-69 23.750394244661475 70-71 22.2594902095481 72-73 22.108692696688276 74-75 19.455858467108268 76-77 21.67797719356702 78-79 20.790674148953766 80-81 21.826634398818413 82-83 22.663939857698654 84-85 19.638212675346843 86-87 21.184713340896895 88-89 20.02764592531662 90-91 21.155468177735255 92-93 21.129493404599298 94-95 20.217653873562956 96-97 19.111354554454735 98-99 18.503164744442135 100-101 20.171817045820735 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2920141.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 80.00185795389453 #Duplication Level Percentage of deduplicated Percentage of total 1 90.2299409569639 72.18562919627317 2 6.755806350002076 10.809541199537694 3 1.576631528340795 3.7840035472785583 4 0.5663469793095837 1.812352423653702 5 0.2811222784221656 1.124515229300264 6 0.15844157161916872 0.7605372064001126 7 0.10331764409062635 0.5785922440668524 8 0.0610709293884681 0.39086302544388385 9 0.04491873057651004 0.3234223712746094 >10 0.2109351058161552 2.8476663758574294 >50 0.008035329079260372 0.42738694022071017 >100 0.0032196481946828895 0.4253465648607359 >500 8.517927868332412E-5 0.046474893772690394 >1k 8.51792786387927E-5 0.11663017691797195 >5k 0.0 0.0 >10k+ 4.258963931939635E-5 4.367038605141556 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 128169 4.389137373845989 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 3.424492173494362E-5 0.0 12-13 0.0 0.0 0.0 8.561230433735904E-5 0.0 14-15 3.424492173494362E-5 0.0 0.0 1.0273476520483086E-4 0.0 16-17 3.424492173494362E-5 1.712246086747181E-5 0.0 1.0273476520483086E-4 0.0 18-19 3.424492173494362E-5 3.424492173494362E-5 0.0 1.0273476520483086E-4 0.0 20-21 3.424492173494362E-5 3.424492173494362E-5 0.0 1.0273476520483086E-4 0.0 22-23 3.424492173494362E-5 6.848984346988724E-5 0.0 1.0273476520483086E-4 0.0 24-25 3.424492173494362E-5 6.848984346988724E-5 0.0 1.0273476520483086E-4 0.0 26-27 3.424492173494362E-5 6.848984346988724E-5 0.0 1.541021478072463E-4 0.0 28-29 3.424492173494362E-5 6.848984346988724E-5 0.0 4.1093906081932346E-4 0.0 30-31 3.424492173494362E-5 6.848984346988724E-5 0.0 0.0011129599563856677 0.0 32-33 3.424492173494362E-5 6.848984346988724E-5 0.0 0.0026882263561930743 0.0 34-35 3.424492173494362E-5 6.848984346988724E-5 0.0 0.005153860721109015 0.0 36-37 3.424492173494362E-5 6.848984346988724E-5 0.0 0.009708435311856518 0.0 38-39 3.424492173494362E-5 6.848984346988724E-5 0.0 0.01703684856313445 0.0 40-41 3.424492173494362E-5 6.848984346988724E-5 0.0 0.025598078996870357 0.0 42-43 3.424492173494362E-5 6.848984346988724E-5 0.0 0.033628513143714636 0.0 44-45 3.424492173494362E-5 6.848984346988724E-5 0.0 0.04292600939475183 0.0 46-47 3.424492173494362E-5 6.848984346988724E-5 0.0 0.05592195719316293 0.0 48-49 3.424492173494362E-5 6.848984346988724E-5 0.0 0.07035619170444167 0.0 50-51 3.424492173494362E-5 6.848984346988724E-5 0.0 0.08725606058063634 0.0 52-53 3.424492173494362E-5 6.848984346988724E-5 0.0 0.10549148140449383 0.0 54-55 3.424492173494362E-5 8.561230433735904E-5 0.0 0.12600418952372505 0.0 56-57 3.424492173494362E-5 1.0273476520483086E-4 0.0 0.1504379411816073 0.0 58-59 3.424492173494362E-5 1.0273476520483086E-4 0.0 0.1778510010304297 0.0 60-61 3.424492173494362E-5 1.0273476520483086E-4 0.0 0.2075584706354933 0.0 62-63 5.136738260241543E-5 1.0273476520483086E-4 0.0 0.2392007783185812 0.0 64-65 6.848984346988724E-5 1.0273476520483086E-4 0.0 0.2722300053319343 0.0 66-67 6.848984346988724E-5 1.0273476520483086E-4 0.0 0.31152605302278213 0.0 68-69 6.848984346988724E-5 1.0273476520483086E-4 0.0 0.35609581866081125 0.0 70-71 6.848984346988724E-5 1.0273476520483086E-4 0.0 0.4057852000982144 0.0 72-73 6.848984346988724E-5 1.0273476520483086E-4 0.0 0.45537184677041276 0.0 74-75 6.848984346988724E-5 1.0273476520483086E-4 0.0 0.5068933315206354 0.0 76-77 6.848984346988724E-5 1.0273476520483086E-4 0.0 0.562438594574714 0.0 78-79 6.848984346988724E-5 1.0273476520483086E-4 0.0 0.6291134571926493 0.0 80-81 6.848984346988724E-5 1.0273476520483086E-4 0.0 0.6992984242884162 0.0 82-83 6.848984346988724E-5 1.0273476520483086E-4 0.0 0.7763494981920394 0.0 84-85 6.848984346988724E-5 1.0273476520483086E-4 0.0 0.8547874914259277 0.0 86-87 6.848984346988724E-5 1.0273476520483086E-4 0.0 0.9394751828764434 0.0 88-89 6.848984346988724E-5 1.0273476520483086E-4 0.0 1.0345733305343816 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2045 0.0 57.096848 1 GTATCAA 3900 0.0 45.828053 1 ATCAACG 4610 0.0 38.665997 3 TCAACGC 4705 0.0 37.135185 4 CAACGCA 4875 0.0 36.03388 5 TATCAAC 5035 0.0 35.08416 2 AACGCAG 5425 0.0 33.31252 6 ACGCAGA 6195 0.0 28.635252 7 CGCAGAG 6155 0.0 28.143848 8 TACATGG 4320 0.0 25.601267 2 GTACATG 4410 0.0 25.315617 1 ACATGGG 4415 0.0 24.40675 3 GCAGAGT 6805 0.0 23.616278 9 GAGTACT 4905 0.0 20.853498 12-13 CAGAGTA 6695 0.0 20.12686 10-11 AGAGTAC 6095 0.0 19.88984 10-11 GTGTAGC 1275 0.0 19.27976 1 GTACTTT 5760 0.0 17.592024 14-15 AGTACTT 5345 0.0 17.4298 12-13 GAGTACA 2985 0.0 17.327967 1 >>END_MODULE