FastQCFastQC Report
Sat 18 Jun 2016
SRR1931008_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931008_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2920141
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71510.24488543532658183No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61740.2114281467915419No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36530.12509669909774904No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40900.055.8919831
GTATCAA76300.040.175651
ATCAACG89950.033.54833
CAACGCA92350.032.5702365
TCAACGC93700.032.154384
AACGCAG96950.031.171686
TATCAAC97750.031.0187442
ACGCAGA111050.026.8720447
CGCAGAG111650.026.642658
GTGGTAT21400.023.0965611
GCAGAGT127050.023.0398229
ACATGGG71100.022.725183
GTACATG77400.022.5347581
TACATGG76000.022.511752
GAGTACT91250.020.87474812-13
GGCGTGC9100.020.8538978
TGGTATC21900.020.832792
AGAGTAC117500.020.67286710-11
CAGAGTA124500.020.31077210-11
GCGTGCG9350.019.281499
Produced by FastQC (version 0.11.5)