##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931008_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2920141 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.292183151430017 31.0 31.0 34.0 30.0 34.0 2 31.449074548112574 31.0 31.0 34.0 30.0 34.0 3 31.515098414768328 31.0 31.0 34.0 30.0 34.0 4 35.292520463909106 37.0 35.0 37.0 33.0 37.0 5 35.16829187357734 37.0 35.0 37.0 32.0 37.0 6 35.19843117164548 37.0 35.0 37.0 32.0 37.0 7 35.08106868812157 37.0 35.0 37.0 32.0 37.0 8 35.08301962131281 37.0 35.0 37.0 32.0 37.0 9 36.583598531714735 39.0 35.0 39.0 32.0 39.0 10-11 36.52217050478042 38.0 35.0 39.0 32.0 39.0 12-13 36.404259931284145 38.0 35.0 39.0 32.0 39.0 14-15 37.45409690833422 39.0 36.0 41.0 32.0 41.0 16-17 37.40860561185231 39.0 36.0 41.0 32.0 41.0 18-19 37.43949264778653 39.0 36.0 41.0 32.0 41.0 20-21 37.42432831154386 39.0 36.0 41.0 32.0 41.0 22-23 37.320870293591994 39.0 36.0 40.5 31.5 41.0 24-25 37.1920362749607 39.0 36.0 40.0 31.0 41.0 26-27 36.940263843424 39.0 36.0 40.0 31.0 41.0 28-29 36.74996995008118 39.0 35.5 40.0 30.0 41.0 30-31 36.52400277931785 38.0 35.0 40.0 30.0 41.0 32-33 36.35941689117067 38.0 35.0 40.0 30.0 41.0 34-35 36.435037212244204 38.0 35.0 40.0 30.0 41.0 36-37 36.4973312932492 38.0 35.0 40.0 30.0 41.0 38-39 36.47669752933163 38.5 35.0 40.0 30.0 41.0 40-41 36.35801096590884 38.5 35.0 40.0 30.0 41.0 42-43 36.21899370612583 38.0 35.0 40.0 30.0 41.0 44-45 36.04816787956472 38.0 35.0 40.0 29.5 41.0 46-47 35.80666926699773 38.0 35.0 40.0 28.5 41.0 48-49 35.586504726997774 38.0 35.0 40.0 27.5 41.0 50-51 35.32701194908054 38.0 34.0 40.0 27.0 41.0 52-53 35.12335192033535 38.0 34.0 40.0 26.5 41.0 54-55 34.97989668992011 38.0 34.0 40.0 26.0 41.0 56-57 34.8125626468037 38.0 34.0 40.0 26.0 41.0 58-59 34.60518019506593 38.0 33.0 40.0 26.0 41.0 60-61 34.31038672447666 37.5 33.0 40.0 25.0 41.0 62-63 34.07289802102022 37.0 33.0 40.0 25.0 41.0 64-65 33.77062991136387 37.0 32.0 40.0 23.5 41.0 66-67 33.45983259027561 36.0 32.0 39.0 23.0 41.0 68-69 33.11598309807643 36.0 31.5 39.0 22.5 40.0 70-71 32.75082093638629 35.5 31.0 39.0 22.0 40.0 72-73 32.32620924811508 35.0 31.0 38.0 21.5 40.0 74-75 31.816757136042405 35.0 31.0 37.5 20.0 39.5 76-77 30.437731260237094 33.5 28.5 36.0 19.5 39.0 78-79 31.169985627406348 34.0 30.0 36.0 20.0 39.0 80-81 31.13206177372942 34.0 30.0 36.0 20.0 38.5 82-83 30.85121608853819 34.0 30.0 36.0 20.0 37.5 84-85 30.49500007020209 34.0 30.0 35.0 18.0 37.0 86-87 30.13405414327596 34.0 30.0 35.0 17.5 36.5 88-89 29.764511371197486 34.0 29.0 35.0 13.0 36.0 90-91 29.40913195629937 34.0 29.0 35.0 7.5 36.0 92-93 29.073941977459306 34.0 29.0 35.0 4.5 35.5 94-95 28.678679899361025 34.0 29.0 35.0 2.0 35.0 96-97 28.229995743356227 34.0 29.0 35.0 2.0 35.0 98-99 27.709392971092832 33.0 28.5 35.0 2.0 35.0 100-101 26.55105027462715 32.5 25.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3255903143709844 1101 2 0.36316495002893134 1101 3 0.24908138015443626 1101 4 0.3453623956947851 1101 5 0.4371660465793923 1101 6 0.35767408851353366 1101 7 0.405487598729124 1101 8 0.36932632607325644 1101 9 0.5160015942983307 1101 10-11 0.48625796139540256 1101 12-13 0.4510372242186591 1101 14-15 0.4608826304021889 1101 16-17 0.5457799220506843 1101 18-19 0.579695607811253 1101 20-21 0.5879562639637257 1101 22-23 0.5960992094501947 1101 24-25 0.5627872490309045 1101 26-27 0.5073319800400569 1101 28-29 0.6394531322064196 1101 30-31 0.545687678532957 1101 32-33 0.5922244479294321 1101 34-35 0.5451046965529045 1101 36-37 0.6031613919124794 1101 38-39 0.6842404666993573 1101 40-41 0.7227052715024769 1101 42-43 0.66932244307052 1101 44-45 0.7056112463838602 1101 46-47 0.6241523502705988 1101 48-49 0.7084407755639859 1101 50-51 0.6565402552168464 1101 52-53 0.5234163290114324 1101 54-55 0.5192007647511403 1101 56-57 0.6243503953327334 1101 58-59 0.6173282675022378 1101 60-61 0.41547458727994524 1101 62-63 0.4744276113750274 1101 64-65 0.5000092386617183 1101 66-67 0.47380188274953383 1101 68-69 0.5111700348407311 1101 70-71 0.4952954774655822 1101 72-73 0.44082362704691747 1101 74-75 0.4279285720993862 1101 76-77 0.4577194267021163 1101 78-79 0.5136546542803302 1101 80-81 0.4854319031549963 1101 82-83 0.5221735625269162 1101 84-85 0.5277625593656765 1101 86-87 0.696603547780402 1101 88-89 0.6171906760780139 1101 90-91 0.6179898247160693 1101 92-93 0.6163650148113327 1101 94-95 0.7973289314091119 1101 96-97 0.8305483702590486 1101 98-99 0.8691509386596188 1101 100-101 1.1164473256129206 1102 1 0.36139196863372547 1102 2 0.352088101551427 1102 3 0.385054664510875 1102 4 0.2013181293031323 1102 5 0.2934598259578465 1102 6 0.41374311313672507 1102 7 0.3550804507880869 1102 8 0.3978353726415662 1102 9 0.5185270212677437 1102 10-11 0.4298250417540288 1102 12-13 0.434516769218412 1102 14-15 0.5538846595681619 1102 16-17 0.536300267414461 1102 18-19 0.5674137609805285 1102 20-21 0.46301303203223654 1102 22-23 0.46696238269498025 1102 24-25 0.5726706867491487 1102 26-27 0.5073714224272052 1102 28-29 0.4607654138526911 1102 30-31 0.595557563223025 1102 32-33 0.769351963607221 1102 34-35 0.706066515876465 1102 36-37 0.7621439610514216 1102 38-39 0.7151295382166509 1102 40-41 0.7253882451821454 1102 42-43 0.6322575182272061 1102 44-45 0.7586930115666135 1102 46-47 0.6819345560938999 1102 48-49 0.6664998134638864 1102 50-51 0.7313546643008095 1102 52-53 0.6843569786921648 1102 54-55 0.7065777301296308 1102 56-57 0.6599601857800153 1102 58-59 0.8491910088863079 1102 60-61 0.6388487475693481 1102 62-63 0.3271548630339538 1102 64-65 0.5510657375415136 1102 66-67 0.4512929814718234 1102 68-69 0.5213039428593049 1102 70-71 0.6968181095964638 1102 72-73 0.5738899851864758 1102 74-75 0.49754527677484006 1102 76-77 0.5946561526452712 1102 78-79 0.6986704245500412 1102 80-81 0.6313170371187269 1102 82-83 0.47062815631712596 1102 84-85 0.5740272538832727 1102 86-87 0.5035985771482636 1102 88-89 0.641811750613428 1102 90-91 0.5487454829159191 1102 92-93 0.5281562384567984 1102 94-95 0.675454406333337 1102 96-97 0.7108301404333837 1102 98-99 0.7937370944262945 1102 100-101 1.0246629965858638 1103 1 0.35795925045482235 1103 2 0.36314438505461766 1103 3 0.3243396262089071 1103 4 0.2813398511471661 1103 5 0.3567435464076496 1103 6 0.4404334367394611 1103 7 0.38051427205662236 1103 8 0.36644678502123185 1103 9 0.49432344348303303 1103 10-11 0.4166496363380787 1103 12-13 0.3983617099442398 1103 14-15 0.4243809126154474 1103 16-17 0.5185383342923728 1103 18-19 0.5477110700040484 1103 20-21 0.5275032431468532 1103 22-23 0.5385903704019128 1103 24-25 0.5200698511293353 1103 26-27 0.5705806701623573 1103 28-29 0.5748086361383287 1103 30-31 0.5106273431215556 1103 32-33 0.5579964381676277 1103 34-35 0.5322418609297515 1103 36-37 0.538763897860207 1103 38-39 0.4914116511018207 1103 40-41 0.611231216385228 1103 42-43 0.5977727288569596 1103 44-45 0.5701809971074638 1103 46-47 0.5167552735205518 1103 48-49 0.46343750851212206 1103 50-51 0.4085296107861396 1103 52-53 0.5673493392189926 1103 54-55 0.7045737767017144 1103 56-57 0.789179498172949 1103 58-59 0.7717453238473837 1103 60-61 0.5934162512287031 1103 62-63 0.4036320759086749 1103 64-65 0.5138310690600463 1103 66-67 0.5421143365200223 1103 68-69 0.5171381015486176 1103 70-71 0.5585804619884911 1103 72-73 0.4906430554562391 1103 74-75 0.5923599928011747 1103 76-77 0.6487531979471264 1103 78-79 0.7432648713152901 1103 80-81 0.7504887436323813 1103 82-83 0.738524706906599 1103 84-85 0.7023987649700665 1103 86-87 0.7447340359884471 1103 88-89 0.8524924998688626 1103 90-91 0.8226181556027257 1103 92-93 0.6396608509709587 1103 94-95 0.673919298686215 1103 96-97 0.8307988738785959 1103 98-99 0.48925450164702156 1103 100-101 0.7951975265382778 1104 1 0.24575018448470232 1104 2 0.3851050827521121 1104 3 0.35165117330092954 1104 4 0.24076225646856386 1104 5 0.2303590189283682 1104 6 0.2833183371392707 1104 7 0.30778548611300494 1104 8 0.32784454877847224 1104 9 0.37815693846861365 1104 10-11 0.4450120830808686 1104 12-13 0.4572569048712367 1104 14-15 0.47474775117766654 1104 16-17 0.5203592428100166 1104 18-19 0.49822611646707315 1104 20-21 0.44069790766242534 1104 22-23 0.42812058656777907 1104 24-25 0.3794458414494031 1104 26-27 0.5154700584126388 1104 28-29 0.5244805300944577 1104 30-31 0.5517307077289928 1104 32-33 0.6015690808457421 1104 34-35 0.5555833248909039 1104 36-37 0.5406806773633406 1104 38-39 0.6408657146551562 1104 40-41 0.5853628557310628 1104 42-43 0.46846133541460233 1104 44-45 0.4753391442206052 1104 46-47 0.3905669694606857 1104 48-49 0.36045126335768174 1104 50-51 0.2766058530276325 1104 52-53 0.28978523049905647 1104 54-55 0.27151109084164915 1104 56-57 0.5092675906444342 1104 58-59 0.48674825279401546 1104 60-61 0.4922240259014714 1104 62-63 0.31525679301724097 1104 64-65 0.31686805061248435 1104 66-67 0.18471946649803783 1104 68-69 0.1595545305384647 1104 70-71 0.34669854175187 1104 72-73 0.3207356613864789 1104 74-75 0.29507991187901794 1104 76-77 0.32999529539301875 1104 78-79 0.5334516196723555 1104 80-81 0.46733914968361745 1104 82-83 0.39263163426551984 1104 84-85 0.5517411124167602 1104 86-87 0.4949201761489448 1104 88-89 0.5924895869273996 1104 90-91 0.44955326102879667 1104 92-93 0.4155988341271133 1104 94-95 0.5598518720939367 1104 96-97 0.6378237043037878 1104 98-99 0.7089832779875316 1104 100-101 0.9569560468920564 1105 1 0.25370960021110633 1105 2 0.3250502290079389 1105 3 0.33646143432478226 1105 4 0.18600659928571872 1105 5 0.24910612942305477 1105 6 0.2659238275117559 1105 7 0.31438105532993177 1105 8 0.3651763327122026 1105 9 0.368013369101746 1105 10-11 0.39742927142038553 1105 12-13 0.46064840316564926 1105 14-15 0.4647663064874834 1105 16-17 0.479626276834324 1105 18-19 0.5400108623780255 1105 20-21 0.5554749940414538 1105 22-23 0.49510681690352243 1105 24-25 0.4816087533210549 1105 26-27 0.4786905604680882 1105 28-29 0.5675972598616639 1105 30-31 0.4355026211125619 1105 32-33 0.566056904314145 1105 34-35 0.5851465566385983 1105 36-37 0.666838666142695 1105 38-39 0.5783405681494287 1105 40-41 0.4588853032826421 1105 42-43 0.43550379396192085 1105 44-45 0.5218586179245506 1105 46-47 0.457670966661766 1105 48-49 0.372175782606476 1105 50-51 0.3647188967231969 1105 52-53 0.33732618337980824 1105 54-55 0.352450351877998 1105 56-57 0.5059851126046837 1105 58-59 0.5070460124121681 1105 60-61 0.5720012372320866 1105 62-63 0.3729050793063067 1105 64-65 0.4017282922688423 1105 66-67 0.4584549564956646 1105 68-69 0.30880763246146614 1105 70-71 0.27451932369886123 1105 72-73 0.3209477666956744 1105 74-75 0.39584867648273914 1105 76-77 0.3795320374054896 1105 78-79 0.4920104906098679 1105 80-81 0.47504834986854405 1105 82-83 0.5269105386900605 1105 84-85 0.517319450810632 1105 86-87 0.5375586537388806 1105 88-89 0.6526394038733336 1105 90-91 0.53529066278562 1105 92-93 0.5038349551302268 1105 94-95 0.5582549908854091 1105 96-97 0.5480678596935746 1105 98-99 0.6452195489325412 1105 100-101 0.7233006508580218 1106 1 0.180068866825966 1106 2 0.18556622195204753 1106 3 0.29101240245402593 1106 4 0.06651264161285297 1106 5 0.1591243859269298 1106 6 0.19868068295231467 1106 7 0.25237123629135993 1106 8 0.27081734570145954 1106 9 0.22041734770380117 1106 10-11 0.23989147346725304 1106 12-13 0.32554996607040465 1106 14-15 0.4060240521381999 1106 16-17 0.42666165086650665 1106 18-19 0.36057399739802776 1106 20-21 0.32094440000606284 1106 22-23 0.26295779202286695 1106 24-25 0.39252523803945394 1106 26-27 0.4254553764022333 1106 28-29 0.448163026449798 1106 30-31 0.3623896404915925 1106 32-33 0.3969742319567118 1106 34-35 0.42825462362845457 1106 36-37 0.26279571401666857 1106 38-39 0.3207534282939193 1106 40-41 0.22026364876641935 1106 42-43 0.30060046297231935 1106 44-45 0.3909284408282403 1106 46-47 0.31535165054053493 1106 48-49 0.4115089884732299 1106 50-51 0.19096889158341668 1106 52-53 0.20752202961399746 1106 54-55 0.2391605216552577 1106 56-57 0.39503121027697574 1106 58-59 0.3415302456184435 1106 60-61 0.3067235451173289 1106 62-63 0.16331918122200761 1106 64-65 0.12304173259186513 1106 66-67 -0.015230904171026793 1106 68-69 0.06046233371681353 1106 70-71 0.148663036101361 1106 72-73 0.2842849857038203 1106 74-75 0.36587460154060736 1106 76-77 0.359555248658733 1106 78-79 0.3570234839774926 1106 80-81 0.22906097728372998 1106 82-83 0.31191728208338176 1106 84-85 0.37970879131498947 1106 86-87 0.23460451292531204 1106 88-89 0.3281627364254227 1106 90-91 0.2635197819093058 1106 92-93 0.34219653670266936 1106 94-95 0.4107477849117487 1106 96-97 0.4710071156999227 1106 98-99 0.5708912155786514 1106 100-101 0.6992521272088581 1107 1 0.14599243825016828 1107 2 0.23328239681253748 1107 3 0.21949441260345282 1107 4 0.04981094059485258 1107 5 0.16511943966099807 1107 6 0.19470715546547268 1107 7 0.22294462391264602 1107 8 0.1934971779443444 1107 9 0.23380549909298054 1107 10-11 0.20800498068786766 1107 12-13 0.2981736278159488 1107 14-15 0.23283144747860263 1107 16-17 0.25461233500480773 1107 18-19 0.3410990437310417 1107 20-21 0.40141541664109326 1107 22-23 0.3026242554835008 1107 24-25 0.29164506367644094 1107 26-27 0.34639840871199823 1107 28-29 0.3457118111893678 1107 30-31 0.4394775824901984 1107 32-33 0.4648364501102833 1107 34-35 0.3785435295984314 1107 36-37 0.4308344971455398 1107 38-39 0.4463071307554287 1107 40-41 0.4496460179584858 1107 42-43 0.47055291683809486 1107 44-45 0.39043931914724084 1107 46-47 0.44290700039811526 1107 48-49 0.4112622868333773 1107 50-51 0.36792746813534194 1107 52-53 0.44725740642526546 1107 54-55 0.44568690140044254 1107 56-57 0.49053247334858696 1107 58-59 0.5508083680762397 1107 60-61 0.5062227000112998 1107 62-63 0.3229239284955412 1107 64-65 0.4048246514596485 1107 66-67 0.345941825715137 1107 68-69 0.2914707929325431 1107 70-71 0.30121792721623564 1107 72-73 0.30143136133050774 1107 74-75 0.3993543587535733 1107 76-77 0.3608264757037567 1107 78-79 0.5384477679820563 1107 80-81 0.410369862008821 1107 82-83 0.47066305910179906 1107 84-85 0.5879704418509029 1107 86-87 0.5253551383445547 1107 88-89 0.5268937521225787 1107 90-91 0.5230972085053729 1107 92-93 0.4987277077606933 1107 94-95 0.7286567948226264 1107 96-97 0.7195564692804588 1107 98-99 0.7124609516078948 1107 100-101 0.9118620470614047 1108 1 0.15261106742559605 1108 2 0.16066966132397198 1108 3 0.18819397415866845 1108 4 0.09346241485571483 1108 5 0.1471405837435853 1108 6 0.19943729149346723 1108 7 0.2150617461004245 1108 8 0.16625166810440817 1108 9 0.30576840627948343 1108 10-11 0.25057037183538 1108 12-13 0.3064326896972105 1108 14-15 0.29581137382184153 1108 16-17 0.25254608567459513 1108 18-19 0.2825122637152191 1108 20-21 0.2509994037255794 1108 22-23 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-1.3342879293632492 2316 84-85 -1.4479600568075846 2316 86-87 -1.3211514388421826 2316 88-89 -1.4790792963937527 2316 90-91 -1.3754876270249312 2316 92-93 -1.361957104210223 2316 94-95 -1.777704240235117 2316 96-97 -1.5145886908662014 2316 98-99 -1.6915682757585095 2316 100-101 -2.018366707744825 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 13.0 4 38.0 5 61.0 6 140.0 7 387.0 8 989.0 9 1868.0 10 3520.0 11 5454.0 12 7752.0 13 10492.0 14 12811.0 15 16049.0 16 19022.0 17 21929.0 18 18917.0 19 14771.0 20 14282.0 21 15203.0 22 16644.0 23 19684.0 24 24352.0 25 30924.0 26 39887.0 27 51342.0 28 65344.0 29 82129.0 30 100750.0 31 121182.0 32 146350.0 33 177115.0 34 218299.0 35 271390.0 36 354103.0 37 484248.0 38 466212.0 39 86476.0 40 9.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.85687734951865 21.931580441593923 15.180274882337851 23.031267326549575 2 14.811388860730363 22.003708249656846 41.19246185585087 21.99244103376192 3 18.404426437395273 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20.424727375337827 28.1358856380465 30.703714763579182 20.73567222303649 24-25 20.336468933992805 28.353088793238364 30.872470695563642 20.43797157720519 26-27 20.336862998853277 28.62857760261392 30.627629334265276 20.406930064267527 28-29 20.319262582164423 28.6587899773829 30.714465133552878 20.3074823068998 30-31 20.630794399772874 28.439567349200484 30.63753080957125 20.292107441455393 32-33 20.462400482544165 28.5668167652586 30.468872424645717 20.501910327551514 34-35 20.535921770135126 28.55339378704614 30.632267199177193 20.27841724364155 36-37 20.53682068039362 28.43057567698023 30.714181633382342 20.318422009243804 38-39 20.39408472249642 28.687626781446806 30.668871816990556 20.249416679066215 40-41 20.657109546053608 28.630010395222204 30.489695271535155 20.22318478718903 42-43 20.580475882887626 28.58703387978741 30.348391016626515 20.484099220698447 44-45 20.538434219399154 28.914682673547244 30.18110825980655 20.365774847247057 46-47 20.62536335684789 28.77733149874693 29.88318639232351 20.714118752081674 48-49 20.66042449891586 28.901791515158987 29.866508848664935 20.57127513726022 50-51 20.719194677612883 29.074611709148733 29.608119779440333 20.59807383379805 52-53 20.766567461371817 29.132638770562874 29.449042064942045 20.65175170312327 54-55 20.748298154728776 29.025114526694352 29.573027357965493 20.653559960611382 56-57 20.736967197956123 29.172191894353993 29.440690562134836 20.650150345555048 58-59 20.687086931980094 29.13811268331178 29.605365911133518 20.569434473574606 60-61 20.703395207393275 29.192896583397804 29.58384884573509 20.51985936347383 62-63 20.671874227687372 29.147147180132137 29.564453436070366 20.616525156110125 64-65 20.53157098440438 29.02839959734412 29.654146746271014 20.785882671980488 66-67 20.59387408888835 29.17587941596162 29.42963376328357 20.80061273186647 68-69 20.624012509429704 29.17431413120998 29.48071620261225 20.720957156748064 70-71 20.658702828589842 28.89318325551701 29.513408406565915 20.934705509327234 72-73 20.73031904275512 29.04022041931255 29.475680955872917 20.75377958205941 74-75 20.623232350281484 29.196011626062653 29.31486772366884 20.865888299987024 76-77 20.57169483348671 29.149055970617844 29.354722823256207 20.924526372639242 78-79 20.604240222783528 29.214481888392825 29.296828779938934 20.884449108884713 80-81 20.579369383082767 29.26568088076584 29.217018838648347 20.937930897503048 82-83 20.655560810248545 29.18653585563163 29.380122398199266 20.77778093592056 84-85 20.775024219720898 29.13742863786372 29.200781743073367 20.886765399342018 86-87 20.59493017631683 29.105289778815475 29.278141021272603 21.021639023595093 88-89 20.703702682697177 29.142032426875495 29.20103645733363 20.95322843309369 90-91 20.663603147036955 29.128602412436088 29.358708855930217 20.84908558459674 92-93 20.645701020767277 29.17152674965128 29.219015847110107 20.963756382471335 94-95 20.44398722424168 29.147639527455162 29.306095094230034 21.102278154073126 96-97 20.517677146014034 29.014408724511714 29.354888917159975 21.11302521231427 98-99 20.39593293611507 29.10607741201538 29.25797076237072 21.24001888949883 100-101 20.455981406377294 29.12366217932627 29.1596022246871 21.260754189609337 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 163.0 1 120.0 2 95.5 3 151.5 4 151.0 5 140.5 6 270.5 7 577.5 8 992.5 9 1260.0 10 1286.5 11 1276.0 12 1451.0 13 1719.0 14 1966.0 15 2301.5 16 2675.5 17 3088.5 18 3643.0 19 4215.5 20 4921.5 21 5951.5 22 7361.5 23 9576.0 24 12673.0 25 16559.0 26 21959.5 27 28440.5 28 34989.0 29 42294.5 30 50059.5 31 58379.0 32 68406.0 33 78204.5 34 87065.0 35 95350.0 36 105498.5 37 114883.5 38 118556.5 39 120250.0 40 122299.0 41 122695.0 42 122387.5 43 123100.5 44 123855.5 45 124221.0 46 121035.0 47 116090.5 48 112791.5 49 108425.0 50 100540.5 51 90939.0 52 81543.0 53 71838.5 54 62328.5 55 51944.0 56 41163.0 57 31625.0 58 24276.5 59 19005.5 60 14152.5 61 9844.5 62 6415.5 63 3806.0 64 2190.0 65 1243.0 66 659.0 67 352.5 68 192.5 69 106.0 70 67.5 71 42.5 72 31.0 73 26.0 74 20.5 75 13.5 76 10.0 77 8.0 78 3.5 79 1.5 80 2.5 81 3.0 82 1.5 83 1.0 84 1.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.004383349982072783 2 0.005650412086265697 3 0.001712246086747181 4 0.16797134110989848 5 0.001438286712867632 6 5.136738260241543E-4 7 3.424492173494362E-5 8 5.479187477590979E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 3.082042956144926E-4 24-25 6.848984346988725E-4 26-27 0.002790961121397905 28-29 1.883470695421899E-4 30-31 0.0064380452861694006 32-33 0.0210777493278578 34-35 0.013235662250555709 36-37 7.705107390362314E-4 38-39 2.5683691301207716E-4 40-41 0.0016951236258797094 42-43 0.011882987842025437 44-45 0.007876331999037034 46-47 0.010907007572579544 48-49 0.011026864798651845 50-51 0.18093304398657464 52-53 0.11280277219490428 54-55 0.01767037961523091 56-57 0.006883229268723668 58-59 0.017978583910845402 60-61 0.12138112508950766 62-63 0.23331065178017088 64-65 0.23914941093597877 66-67 0.2494571323781968 68-69 0.24445737380489502 70-71 0.23161552815429118 72-73 0.15776635443288525 74-75 0.2525562977952092 76-77 0.19584670740214255 78-79 0.0060442286862175485 80-81 1.712246086747181E-5 82-83 0.0 84-85 0.0 86-87 0.0 88-89 5.1367382602415425E-5 90-91 0.0064380452861694006 92-93 0.017927216528242983 94-95 0.0027224712779280176 96-97 1.712246086747181E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2920141.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.618422507694625 #Duplication Level Percentage of deduplicated Percentage of total 1 73.34514265546491 42.99376561065177 2 15.847964687828641 18.579653799163285 3 5.1065561955170935 8.9801480588432 4 2.167784804089988 5.082885022076278 5 1.1327331168933177 3.3199514217255164 6 0.6316549164305836 2.221596886223429 7 0.39979218890068574 1.6404631210979521 8 0.2650476421333016 1.2429339736990497 9 0.1818069987983467 0.9591515523375673 >10 0.8375264778294254 8.614274589113355 >50 0.05433226592815381 2.1901039197131147 >100 0.028133297492476263 2.970764196005064 >500 0.001172886686996354 0.4660406335923199 >1k 2.345773373095589E-4 0.2802019033174354 >5k 1.1728866865477945E-4 0.4580653124407835 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7151 0.24488543532658183 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6174 0.2114281467915419 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3653 0.12509669909774904 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 3.424492173494362E-5 0.0 8 0.0 0.0 0.0 3.424492173494362E-5 0.0 9 0.0 0.0 0.0 3.424492173494362E-5 0.0 10-11 0.0 0.0 0.0 5.136738260241543E-5 0.0 12-13 0.0 0.0 0.0 6.848984346988724E-5 3.424492173494362E-5 14-15 0.0 0.0 0.0 6.848984346988724E-5 3.424492173494362E-5 16-17 0.0 0.0 0.0 6.848984346988724E-5 3.424492173494362E-5 18-19 0.0 0.0 0.0 6.848984346988724E-5 3.424492173494362E-5 20-21 0.0 0.0 0.0 6.848984346988724E-5 3.424492173494362E-5 22-23 0.0 0.0 0.0 6.848984346988724E-5 3.424492173494362E-5 24-25 1.712246086747181E-5 0.0 0.0 1.0273476520483086E-4 3.424492173494362E-5 26-27 3.424492173494362E-5 0.0 0.0 1.712246086747181E-4 3.424492173494362E-5 28-29 3.424492173494362E-5 0.0 0.0 4.965513651566825E-4 3.424492173494362E-5 30-31 3.424492173494362E-5 0.0 0.0 0.00198620546062673 3.424492173494362E-5 32-33 3.424492173494362E-5 0.0 0.0 0.005616167164530754 3.424492173494362E-5 34-35 3.424492173494362E-5 0.0 0.0 0.010615925737832523 3.424492173494362E-5 36-37 3.424492173494362E-5 0.0 0.0 0.019844932145399827 3.424492173494362E-5 38-39 3.424492173494362E-5 0.0 0.0 0.035546228760871476 3.424492173494362E-5 40-41 3.424492173494362E-5 0.0 0.0 0.05494597692371704 3.424492173494362E-5 42-43 3.424492173494362E-5 0.0 0.0 0.07376356141706855 3.424492173494362E-5 44-45 3.424492173494362E-5 0.0 0.0 0.09660492421427595 3.424492173494362E-5 46-47 3.424492173494362E-5 0.0 0.0 0.12521655632382134 3.424492173494362E-5 48-49 3.424492173494362E-5 0.0 0.0 0.15590000619833083 3.424492173494362E-5 50-51 3.424492173494362E-5 0.0 0.0 0.1894429070377081 3.424492173494362E-5 52-53 3.424492173494362E-5 0.0 0.0 0.22358509400744692 3.424492173494362E-5 54-55 3.424492173494362E-5 0.0 0.0 0.25995320088995705 3.424492173494362E-5 56-57 3.424492173494362E-5 0.0 0.0 0.30524210988441997 3.424492173494362E-5 58-59 3.424492173494362E-5 0.0 0.0 0.3554451651478473 3.424492173494362E-5 60-61 3.424492173494362E-5 0.0 0.0 0.40943228426298595 3.424492173494362E-5 62-63 3.424492173494362E-5 0.0 0.0 0.46607338481258265 3.424492173494362E-5 64-65 3.424492173494362E-5 0.0 0.0 0.5279710808484933 3.424492173494362E-5 66-67 3.424492173494362E-5 1.712246086747181E-5 0.0 0.6025907653089354 3.424492173494362E-5 68-69 3.424492173494362E-5 3.424492173494362E-5 0.0 0.6853778636031617 3.424492173494362E-5 70-71 3.424492173494362E-5 3.424492173494362E-5 0.0 0.7779932544353166 3.424492173494362E-5 72-73 3.424492173494362E-5 3.424492173494362E-5 0.0 0.8756768936842434 3.424492173494362E-5 74-75 3.424492173494362E-5 3.424492173494362E-5 0.0 0.9756720651502787 3.424492173494362E-5 76-77 3.424492173494362E-5 5.136738260241543E-5 0.0 1.0823963637372305 3.424492173494362E-5 78-79 3.424492173494362E-5 6.848984346988724E-5 0.0 1.212509943869149 3.424492173494362E-5 80-81 3.424492173494362E-5 6.848984346988724E-5 0.0 1.355362634886466 3.424492173494362E-5 82-83 3.424492173494362E-5 6.848984346988724E-5 0.0 1.5098757217545318 3.424492173494362E-5 84-85 3.424492173494362E-5 6.848984346988724E-5 0.0 1.6675907088048145 3.424492173494362E-5 86-87 3.424492173494362E-5 6.848984346988724E-5 0.0 1.83890092978387 3.424492173494362E-5 88-89 3.424492173494362E-5 6.848984346988724E-5 0.0 2.034302453203458 3.424492173494362E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4090 0.0 55.891983 1 GTATCAA 7630 0.0 40.17565 1 ATCAACG 8995 0.0 33.5483 3 CAACGCA 9235 0.0 32.570236 5 TCAACGC 9370 0.0 32.15438 4 AACGCAG 9695 0.0 31.17168 6 TATCAAC 9775 0.0 31.018744 2 ACGCAGA 11105 0.0 26.872044 7 CGCAGAG 11165 0.0 26.64265 8 GTGGTAT 2140 0.0 23.096561 1 GCAGAGT 12705 0.0 23.039822 9 ACATGGG 7110 0.0 22.72518 3 GTACATG 7740 0.0 22.534758 1 TACATGG 7600 0.0 22.51175 2 GAGTACT 9125 0.0 20.874748 12-13 GGCGTGC 910 0.0 20.853897 8 TGGTATC 2190 0.0 20.83279 2 AGAGTAC 11750 0.0 20.672867 10-11 CAGAGTA 12450 0.0 20.310772 10-11 GCGTGCG 935 0.0 19.28149 9 >>END_MODULE