FastQCFastQC Report
Sat 18 Jun 2016
SRR1931007_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931007_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1907119
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN867354.547959513800659No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15950.056.2960621
GTATCAA30850.044.2412221
ATCAACG35350.037.2309953
TCAACGC35900.036.7981454
CAACGCA37350.035.3569375
TATCAAC40000.033.4702072
AACGCAG40950.033.3576436
ACGCAGA45450.029.1885497
CGCAGAG47250.028.2282228
GCAGAGT51550.024.9129739
GTGGTAT8200.024.403461
TGGTATC9150.022.424192
ACATGGG35200.022.0613253
GTACATG35900.022.010351
AGAGTAC46800.021.03486410-11
GAGTACT33800.020.89404512-13
TACATGG36850.020.6016542
CAGAGTA50250.020.50187510-11
GAGTACA26750.020.3321231
CGTACAC1505.1632046E-420.1703553
Produced by FastQC (version 0.11.5)