##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931007_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1907119 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.91831028897515 30.0 2.0 31.0 2.0 33.0 2 20.486710582821523 28.0 2.0 31.0 2.0 33.0 3 21.25086163999205 28.0 2.0 31.0 2.0 34.0 4 22.731957995279792 32.0 2.0 35.0 2.0 37.0 5 22.041149503518135 32.0 2.0 35.0 2.0 37.0 6 23.145076945906364 32.0 2.0 35.0 2.0 37.0 7 23.40390557694617 32.0 2.0 35.0 2.0 37.0 8 23.93273151806468 32.0 2.0 35.0 2.0 37.0 9 25.4278243780278 33.0 10.0 37.0 2.0 39.0 10-11 25.90136929053719 34.0 10.0 37.5 2.0 39.0 12-13 25.885003505287294 34.0 6.0 38.0 2.0 39.0 14-15 25.902165255550386 34.0 2.0 38.5 2.0 40.0 16-17 24.647321955263408 33.0 2.0 38.0 2.0 40.0 18-19 23.483873056689173 32.0 2.0 38.0 2.0 40.0 20-21 23.12237332856523 31.5 2.0 38.0 2.0 40.0 22-23 22.748805134865734 31.0 2.0 38.0 2.0 40.0 24-25 22.613291567018102 30.0 2.0 38.0 2.0 40.0 26-27 22.347881280612274 30.0 2.0 38.0 2.0 40.0 28-29 22.200157934559932 30.0 2.0 38.0 2.0 40.0 30-31 22.267209335128012 30.0 2.0 38.0 2.0 40.0 32-33 22.172321968372188 30.0 2.0 38.0 2.0 40.0 34-35 21.88687858492312 29.5 2.0 37.5 2.0 40.0 36-37 21.38671367649318 27.0 2.0 37.0 2.0 40.0 38-39 21.33395556333926 27.0 2.0 37.0 2.0 40.0 40-41 21.26219234352969 27.5 2.0 37.0 2.0 39.0 42-43 21.152283889993228 27.5 2.0 37.0 2.0 39.0 44-45 20.781848694287035 26.5 2.0 37.0 2.0 39.0 46-47 20.46116236060781 25.0 2.0 37.0 2.0 39.0 48-49 20.269447527920388 24.5 2.0 36.5 2.0 39.0 50-51 17.77009143110629 12.0 2.0 34.0 2.0 38.5 52-53 19.82476421240625 24.5 2.0 35.5 2.0 38.5 54-55 20.705901676822474 27.0 2.0 37.0 2.0 39.0 56-57 20.723022003346408 27.0 2.0 37.0 2.0 40.0 58-59 20.46086741309798 26.5 2.0 37.0 2.0 40.0 60-61 20.193531184996846 26.0 2.0 36.5 2.0 39.0 62-63 19.760896409715386 25.0 2.0 36.0 2.0 39.0 64-65 19.44423525747476 23.5 2.0 35.5 2.0 39.0 66-67 19.11493829173743 23.0 2.0 35.0 2.0 39.0 68-69 18.69500172773697 22.0 2.0 34.5 2.0 38.0 70-71 18.372111021913156 21.0 2.0 34.0 2.0 38.0 72-73 17.977044694117147 19.0 2.0 34.0 2.0 37.5 74-75 17.53546684816207 17.5 2.0 34.0 2.0 37.0 76-77 17.146442356245203 13.0 2.0 33.0 2.0 36.0 78-79 16.790396928560828 10.0 2.0 33.0 2.0 36.0 80-81 16.37661152764982 7.5 2.0 32.0 2.0 35.5 82-83 15.928161273627918 4.5 2.0 32.0 2.0 35.0 84-85 15.556203624419872 2.0 2.0 31.5 2.0 35.0 86-87 15.07878375707022 2.0 2.0 31.0 2.0 34.5 88-89 14.667415352686433 2.0 2.0 31.0 2.0 34.0 90-91 14.198509374611653 2.0 2.0 31.0 2.0 34.0 92-93 13.645818902753316 2.0 2.0 30.0 2.0 34.0 94-95 13.072583304974676 2.0 2.0 29.5 2.0 34.0 96-97 12.41149687041029 2.0 2.0 29.0 2.0 34.0 98-99 11.69526993333924 2.0 2.0 29.0 2.0 34.0 100-101 10.617734131955059 2.0 2.0 26.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.885124345988732 1101 2 9.513113018338153 1101 3 8.821927468742516 1101 4 10.935292943569408 1101 5 11.729607138694277 1101 6 10.702180375772919 1101 7 10.4184284342363 1101 8 9.570490133177323 1101 9 9.623160789714415 1101 10-11 9.031229366634552 1101 12-13 8.918480266750262 1101 14-15 9.50558214512479 1101 16-17 10.844972284326285 1101 18-19 11.765674958984505 1101 20-21 12.074947994199452 1101 22-23 12.407157543199414 1101 24-25 12.118069570812974 1101 26-27 12.258451395875653 1101 28-29 12.07421595500885 1101 30-31 11.591372945867636 1101 32-33 11.647579981229242 1101 34-35 11.450412761307614 1101 36-37 12.013574728672584 1101 38-39 11.557911731113489 1101 40-41 11.219881999068633 1101 42-43 11.11867796974321 1101 44-45 10.986040565890473 1101 46-47 10.874215172577394 1101 48-49 11.156298236543641 1101 50-51 13.092042131142158 1101 52-53 11.89749941534279 1101 54-55 11.626664986738376 1101 56-57 11.52331682383653 1101 58-59 11.356921334567144 1101 60-61 11.144809017178655 1101 62-63 11.126593136321492 1101 64-65 11.003300065545446 1101 66-67 10.96903112992955 1101 68-69 11.142907455048483 1101 70-71 10.896201285049141 1101 72-73 10.703578747968649 1101 74-75 10.509382603590332 1101 76-77 10.281387488784574 1101 78-79 9.967008524075649 1101 80-81 9.52751988843146 1101 82-83 9.472095056672963 1101 84-85 9.37557426003738 1101 86-87 9.050654548001992 1101 88-89 8.843733471094547 1101 90-91 8.383111953468143 1101 92-93 7.996551860960912 1101 94-95 7.846576364390414 1101 96-97 7.691927003666853 1101 98-99 7.674938060363052 1101 100-101 6.8045751668645735 1102 1 7.927003445456055 1102 2 9.266490068749196 1102 3 8.446154099680555 1102 4 10.70918778414535 1102 5 11.529836112770404 1102 6 10.400316306859708 1102 7 10.099946430324554 1102 8 9.378339328556287 1102 9 9.3595942343098 1102 10-11 8.402961853435233 1102 12-13 8.469597235706583 1102 14-15 9.158896572632763 1102 16-17 10.174694953949366 1102 18-19 11.395697446660165 1102 20-21 11.454112225604423 1102 22-23 11.642032023798077 1102 24-25 11.454868575613713 1102 26-27 11.701431114672644 1102 28-29 11.513248236864253 1102 30-31 11.263998304680488 1102 32-33 11.167181425706204 1102 34-35 10.893370143104566 1102 36-37 11.339848116106392 1102 38-39 11.016693621807054 1102 40-41 10.798657725278872 1102 42-43 10.366630962121405 1102 44-45 10.480480238940235 1102 46-47 10.36347120773081 1102 48-49 10.064348835003415 1102 50-51 12.129689889075692 1102 52-53 10.88504343178916 1102 54-55 10.699420561009429 1102 56-57 10.43916567874146 1102 58-59 10.390914679991536 1102 60-61 10.442598764416335 1102 62-63 10.281815857359327 1102 64-65 10.259715080133223 1102 66-67 9.928846470346752 1102 68-69 9.661994884181375 1102 70-71 9.607180065956477 1102 72-73 9.593243880651748 1102 74-75 9.072459251750331 1102 76-77 8.652472466927357 1102 78-79 8.504594425701384 1102 80-81 8.270160772120192 1102 82-83 8.207821383219809 1102 84-85 7.8244798924242875 1102 86-87 7.843596241030282 1102 88-89 7.63067206231978 1102 90-91 7.459131505348269 1102 92-93 7.504282974234352 1102 94-95 7.211796360049897 1102 96-97 6.965766860858084 1102 98-99 6.4594154842645946 1102 100-101 5.799802979152357 1103 1 8.118596942825299 1103 2 9.586141293111464 1103 3 8.875973690907287 1103 4 11.169977808592066 1103 5 11.965529644336975 1103 6 10.823952301094636 1103 7 10.696363684319028 1103 8 10.046986579300558 1103 9 9.917100971129987 1103 10-11 9.249479037260297 1103 12-13 8.907056128057594 1103 14-15 9.867852828399094 1103 16-17 10.788589928690765 1103 18-19 11.948301198194208 1103 20-21 12.291061840043486 1103 22-23 12.365808621754876 1103 24-25 12.382908104649513 1103 26-27 12.383110036484737 1103 28-29 11.963322260432594 1103 30-31 11.760004952365119 1103 32-33 11.912093480833036 1103 34-35 11.71288097565714 1103 36-37 11.934460408230496 1103 38-39 11.92469897470836 1103 40-41 11.496608720060806 1103 42-43 11.153371167607332 1103 44-45 11.32545589420576 1103 46-47 11.384430248022994 1103 48-49 11.134645000797136 1103 50-51 13.192465557002972 1103 52-53 11.793234870060871 1103 54-55 11.409826958213884 1103 56-57 11.077616770340757 1103 58-59 11.239630982314967 1103 60-61 11.052131394784038 1103 62-63 10.969628576804304 1103 64-65 10.68012643343345 1103 66-67 10.509315744539638 1103 68-69 10.331179862045826 1103 70-71 10.357460783353325 1103 72-73 10.068424065592794 1103 74-75 9.899102120680801 1103 76-77 9.5684116788631 1103 78-79 9.306971629885911 1103 80-81 9.055083565896012 1103 82-83 8.912747731822211 1103 84-85 8.63188405051182 1103 86-87 8.56266759424045 1103 88-89 8.234461475664789 1103 90-91 8.08080127255481 1103 92-93 7.63925764675721 1103 94-95 7.439660346345535 1103 96-97 7.1405419492055895 1103 98-99 6.81221374467763 1103 100-101 6.302805906983814 1104 1 7.863252651624332 1104 2 9.39913007714436 1104 3 8.760753908980014 1104 4 10.935745096534191 1104 5 11.651151693221976 1104 6 10.709632951181483 1104 7 10.133856388547745 1104 8 9.550427571885834 1104 9 9.5635696350252 1104 10-11 8.612934189191225 1104 12-13 8.496174088568633 1104 14-15 9.22241125241326 1104 16-17 10.397904150998759 1104 18-19 11.298899732171805 1104 20-21 11.62127373666198 1104 22-23 11.873690666205608 1104 24-25 11.666397816665349 1104 26-27 11.681915971710566 1104 28-29 11.52698356115469 1104 30-31 11.53805525068545 1104 32-33 11.403839172508711 1104 34-35 11.180669378312164 1104 36-37 11.467995624967454 1104 38-39 11.178054507207602 1104 40-41 10.903102459211809 1104 42-43 10.608995250821039 1104 44-45 10.731377200939441 1104 46-47 10.521765324420002 1104 48-49 10.326622694735384 1104 50-51 12.230960200764333 1104 52-53 11.278608464474221 1104 54-55 11.052524149038355 1104 56-57 10.951343193084707 1104 58-59 10.875318487488922 1104 60-61 10.649657217570763 1104 62-63 10.74296740639415 1104 64-65 10.710589787704617 1104 66-67 10.524522416928892 1104 68-69 10.303818010331415 1104 70-71 9.809497240404049 1104 72-73 9.874600459910983 1104 74-75 9.65597215841381 1104 76-77 9.511324210824128 1104 78-79 9.197126889121801 1104 80-81 8.893957811738499 1104 82-83 8.898093122448579 1104 84-85 8.463451556928836 1104 86-87 8.269769438376398 1104 88-89 8.085505525018283 1104 90-91 7.817757086360716 1104 92-93 7.34165376737854 1104 94-95 7.172550767059862 1104 96-97 6.607424435776801 1104 98-99 6.529611015647861 1104 100-101 5.977528106210666 1105 1 7.941940597728241 1105 2 9.302069038842841 1105 3 8.903013992694682 1105 4 10.896390759627515 1105 5 11.04829865040687 1105 6 10.51050437441932 1105 7 10.198732602414228 1105 8 9.652606079425034 1105 9 9.528120449131261 1105 10-11 8.928250298135058 1105 12-13 8.721055580417715 1105 14-15 9.702358078255259 1105 16-17 10.662601929620248 1105 18-19 11.22674265758971 1105 20-21 11.472684118261643 1105 22-23 12.050328287958788 1105 24-25 11.836150047232305 1105 26-27 12.070430722635535 1105 28-29 11.810000969650066 1105 30-31 11.56283762869467 1105 32-33 11.394415774413083 1105 34-35 11.381426344149606 1105 36-37 11.60842686749 1105 38-39 11.537633249374363 1105 40-41 11.168959978453827 1105 42-43 11.028077432902847 1105 44-45 10.89748400880394 1105 46-47 10.810025832294386 1105 48-49 10.788163820153954 1105 50-51 12.388598349051883 1105 52-53 11.293668914816575 1105 54-55 10.890920688825322 1105 56-57 10.839828116330256 1105 58-59 10.730398431989219 1105 60-61 10.585313604725954 1105 62-63 10.609911815941338 1105 64-65 10.449929741603153 1105 66-67 10.369856167175666 1105 68-69 10.120144089556259 1105 70-71 10.153477622890954 1105 72-73 9.781350413210443 1105 74-75 9.49915856851862 1105 76-77 9.139541524174263 1105 78-79 8.993433034356816 1105 80-81 8.674385773216308 1105 82-83 8.66552304067271 1105 84-85 8.423545028787307 1105 86-87 8.310635381410082 1105 88-89 7.776803830376371 1105 90-91 7.753416506839239 1105 92-93 7.5242162279295375 1105 94-95 7.36136351672449 1105 96-97 6.877410055296435 1105 98-99 6.153501672430609 1105 100-101 5.392607059825339 1106 1 7.912272460965003 1106 2 9.343847694910355 1106 3 8.714269356375755 1106 4 10.660376112035472 1106 5 11.51697783601746 1106 6 10.634199350846224 1106 7 9.819476031361024 1106 8 9.431189296852963 1106 9 8.680589967709029 1106 10-11 8.576475242304046 1106 12-13 8.511811973199539 1106 14-15 9.036958663343412 1106 16-17 10.332311682929454 1106 18-19 11.493669985025754 1106 20-21 11.5649429136046 1106 22-23 11.730260176854689 1106 24-25 11.503517409099327 1106 26-27 11.719621116965868 1106 28-29 11.604135070503428 1106 30-31 11.244816779148255 1106 32-33 11.58285135728093 1106 34-35 11.397404879977184 1106 36-37 11.574889843980554 1106 38-39 11.210285102029214 1106 40-41 11.199737773746563 1106 42-43 10.96590112474647 1106 44-45 10.691487824855724 1106 46-47 10.44833110549714 1106 48-49 10.552076446790036 1106 50-51 12.550132606744278 1106 52-53 11.5975166924413 1106 54-55 11.073647236442177 1106 56-57 10.935797169214446 1106 58-59 10.797911071355418 1106 60-61 10.624293872545756 1106 62-63 10.760997555772533 1106 64-65 10.685274566188593 1106 66-67 10.271724486039261 1106 68-69 10.109000636541584 1106 70-71 9.747141579600601 1106 72-73 9.62532130997267 1106 74-75 9.529958243701707 1106 76-77 9.407632836912985 1106 78-79 9.046272596579993 1106 80-81 8.633514759645301 1106 82-83 8.408765262496011 1106 84-85 7.93007953262847 1106 86-87 8.01334896021921 1106 88-89 7.664445123497783 1106 90-91 7.4265856448560985 1106 92-93 7.101508183738124 1106 94-95 6.713046950514697 1106 96-97 6.1185907190428015 1106 98-99 5.899398074133467 1106 100-101 5.376427005235012 1107 1 7.344734215467625 1107 2 8.50438229825717 1107 3 8.40930352979034 1107 4 10.236509356514524 1107 5 11.176982375946231 1107 6 10.156313518875109 1107 7 10.03047238046113 1107 8 8.590415632717281 1107 9 6.0414481989547255 1107 10-11 7.7087965457982826 1107 12-13 7.835976983569925 1107 14-15 8.254292563438732 1107 16-17 9.709464685820134 1107 18-19 11.010688277438973 1107 20-21 11.31634608698565 1107 22-23 10.713240669257114 1107 24-25 10.8472807913171 1107 26-27 10.860592102737996 1107 28-29 11.255431842341874 1107 30-31 10.923071322358016 1107 32-33 11.15390804757564 1107 34-35 11.152173384926147 1107 36-37 11.738656230134058 1107 38-39 11.155443224248568 1107 40-41 10.771185024294734 1107 42-43 10.615671746546585 1107 44-45 10.193147523655266 1107 46-47 10.165937085370771 1107 48-49 10.467324264197405 1107 50-51 12.256070629425452 1107 52-53 11.070112826891243 1107 54-55 10.818377954018267 1107 56-57 10.344664053544935 1107 58-59 10.552045979178825 1107 60-61 10.488967053572743 1107 62-63 10.518874623834037 1107 64-65 10.413803109167961 1107 66-67 10.276026117156924 1107 68-69 9.987298100062961 1107 70-71 9.781156169590105 1107 72-73 9.547304165179561 1107 74-75 9.59445974348224 1107 76-77 9.496428788501984 1107 78-79 8.945990357860428 1107 80-81 8.757727623975804 1107 82-83 8.685647206363242 1107 84-85 8.542196451834753 1107 86-87 8.341348484839086 1107 88-89 8.105678321751219 1107 90-91 8.022717178355572 1107 92-93 7.422135549398282 1107 94-95 7.224118981296778 1107 96-97 6.785746740451435 1107 98-99 6.386084271243172 1107 100-101 5.796684541686897 1108 1 7.025748082354195 1108 2 8.34091844595844 1108 3 8.264435435203271 1108 4 9.92383192137013 1108 5 11.399859242526574 1108 6 9.88422258720648 1108 7 9.170504546043961 1108 8 6.265651509639319 1108 9 7.565976987633594 1108 10-11 6.151977514575481 1108 12-13 4.877495782592902 1108 14-15 7.387658689270296 1108 16-17 8.861203773272425 1108 18-19 8.410313333801078 1108 20-21 8.765764502384624 1108 22-23 9.598260284475725 1108 24-25 9.535340705140346 1108 26-27 8.041670710696447 1108 28-29 9.74819977031412 1108 30-31 9.970522308423618 1108 32-33 10.504682529225107 1108 34-35 10.253288570987404 1108 36-37 9.99052082122849 1108 38-39 9.607360500349348 1108 40-41 9.921743340689297 1108 42-43 9.186933944270884 1108 44-45 8.688213699398943 1108 46-47 9.502681441323695 1108 48-49 9.971332785424522 1108 50-51 11.642840303115982 1108 52-53 10.809079839084749 1108 54-55 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56-57 -18.467132324330738 2313 58-59 -18.212515939568117 2313 60-61 -17.94372996062207 2313 62-63 -17.520283476998074 2313 64-65 -17.19804221633375 2313 66-67 -16.873508880395693 2313 68-69 -16.464733226417703 2313 70-71 -16.132451270449053 2313 72-73 -15.732920993900223 2313 74-75 -15.322574030379727 2313 76-77 -14.923052366661992 2313 78-79 -14.57016235873282 2313 80-81 -14.167627246312989 2313 82-83 -13.723310209202104 2313 84-85 -13.361131986735979 2313 86-87 -12.879650046732133 2313 88-89 -12.475574737475407 2313 90-91 -12.025251495163763 2313 92-93 -11.466019125100006 2313 94-95 -10.886257915423732 2313 96-97 -10.226245427928399 2313 98-99 -9.538536384655016 2313 100-101 -8.465429995758036 2314 1 -19.690508075281272 2314 2 -18.27780076586422 2314 3 -19.045909392382935 2314 4 -20.47616807862987 2314 5 -19.77994371313845 2314 6 -20.89410253594623 2314 7 -21.143781168241755 2314 8 -21.69641745587792 2314 9 -23.173431879361477 2314 10-11 -23.68792396325703 2314 12-13 -23.685804710017937 2314 14-15 -23.686725547182903 2314 16-17 -22.40825435480639 2314 18-19 -21.238455139105326 2314 20-21 -20.86206800993065 2314 22-23 -20.485976168726243 2314 24-25 -20.365151905697093 2314 26-27 -20.107836678466114 2314 28-29 -19.96140614101593 2314 30-31 -20.025044194039435 2314 32-33 -19.928568702301988 2314 34-35 -19.637351823101266 2314 36-37 -19.12942991768777 2314 38-39 -19.067023736103852 2314 40-41 -19.00830592039445 2314 42-43 -18.89680989809365 2314 44-45 -18.536416842472978 2314 46-47 -18.18722003650882 2314 48-49 -18.004775588959717 2314 50-51 -15.562578416095839 2314 52-53 -17.59165907717141 2314 54-55 -18.46135772880112 2314 56-57 -18.467132324330738 2314 58-59 -18.212515939568117 2314 60-61 -17.94372996062207 2314 62-63 -17.520283476998074 2314 64-65 -17.19804221633375 2314 66-67 -16.873508880395693 2314 68-69 -16.464733226417703 2314 70-71 -16.132451270449053 2314 72-73 -15.732920993900223 2314 74-75 -15.322574030379727 2314 76-77 -14.923052366661992 2314 78-79 -14.57016235873282 2314 80-81 -14.167627246312989 2314 82-83 -13.723310209202104 2314 84-85 -13.361131986735979 2314 86-87 -12.879650046732133 2314 88-89 -12.475574737475407 2314 90-91 -12.025251495163763 2314 92-93 -11.466019125100006 2314 94-95 -10.886257915423732 2314 96-97 -10.226245427928399 2314 98-99 -9.538536384655016 2314 100-101 -8.465429995758036 2315 1 -19.690508075281272 2315 2 -18.27780076586422 2315 3 -19.045909392382935 2315 4 -20.47616807862987 2315 5 -19.77994371313845 2315 6 -20.89410253594623 2315 7 -21.143781168241755 2315 8 -21.69641745587792 2315 9 -23.173431879361477 2315 10-11 -23.68792396325703 2315 12-13 -23.685804710017937 2315 14-15 -23.686725547182903 2315 16-17 -22.40825435480639 2315 18-19 -21.238455139105326 2315 20-21 -20.86206800993065 2315 22-23 -20.485976168726243 2315 24-25 -20.365151905697093 2315 26-27 -20.107836678466114 2315 28-29 -19.96140614101593 2315 30-31 -20.025044194039435 2315 32-33 -19.928568702301988 2315 34-35 -19.637351823101266 2315 36-37 -19.12942991768777 2315 38-39 -19.067023736103852 2315 40-41 -19.00830592039445 2315 42-43 -18.89680989809365 2315 44-45 -18.536416842472978 2315 46-47 -18.18722003650882 2315 48-49 -18.004775588959717 2315 50-51 -15.562578416095839 2315 52-53 -17.59165907717141 2315 54-55 -18.46135772880112 2315 56-57 -18.467132324330738 2315 58-59 -18.212515939568117 2315 60-61 -17.94372996062207 2315 62-63 -17.520283476998074 2315 64-65 -17.19804221633375 2315 66-67 -16.873508880395693 2315 68-69 -16.464733226417703 2315 70-71 -16.132451270449053 2315 72-73 -15.732920993900223 2315 74-75 -15.322574030379727 2315 76-77 -14.923052366661992 2315 78-79 -14.57016235873282 2315 80-81 -14.167627246312989 2315 82-83 -13.723310209202104 2315 84-85 -13.361131986735979 2315 86-87 -12.879650046732133 2315 88-89 -12.475574737475407 2315 90-91 -12.025251495163763 2315 92-93 -11.466019125100006 2315 94-95 -10.886257915423732 2315 96-97 -10.226245427928399 2315 98-99 -9.538536384655016 2315 100-101 -8.465429995758036 2316 1 -19.690508075281272 2316 2 -18.27780076586422 2316 3 -19.045909392382935 2316 4 -20.47616807862987 2316 5 -19.77994371313845 2316 6 -20.89410253594623 2316 7 -21.143781168241755 2316 8 -21.69641745587792 2316 9 -23.173431879361477 2316 10-11 -23.68792396325703 2316 12-13 -23.685804710017937 2316 14-15 -23.686725547182903 2316 16-17 -22.40825435480639 2316 18-19 -21.238455139105326 2316 20-21 -20.86206800993065 2316 22-23 -20.485976168726243 2316 24-25 -20.365151905697093 2316 26-27 -20.107836678466114 2316 28-29 -19.96140614101593 2316 30-31 -20.025044194039435 2316 32-33 -19.928568702301988 2316 34-35 -19.637351823101266 2316 36-37 -19.12942991768777 2316 38-39 -19.067023736103852 2316 40-41 -19.00830592039445 2316 42-43 -18.89680989809365 2316 44-45 -18.536416842472978 2316 46-47 -18.18722003650882 2316 48-49 -18.004775588959717 2316 50-51 -15.562578416095839 2316 52-53 -17.59165907717141 2316 54-55 -18.46135772880112 2316 56-57 -18.467132324330738 2316 58-59 -18.212515939568117 2316 60-61 -17.94372996062207 2316 62-63 -17.520283476998074 2316 64-65 -17.19804221633375 2316 66-67 -16.873508880395693 2316 68-69 -16.464733226417703 2316 70-71 -16.132451270449053 2316 72-73 -15.732920993900223 2316 74-75 -15.322574030379727 2316 76-77 -14.923052366661992 2316 78-79 -14.57016235873282 2316 80-81 -14.167627246312989 2316 82-83 -13.723310209202104 2316 84-85 -13.361131986735979 2316 86-87 -12.879650046732133 2316 88-89 -12.475574737475407 2316 90-91 -12.025251495163763 2316 92-93 -11.466019125100006 2316 94-95 -10.886257915423732 2316 96-97 -10.226245427928399 2316 98-99 -9.538536384655016 2316 100-101 -8.465429995758036 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 460289.0 3 16662.0 4 75765.0 5 37024.0 6 16955.0 7 17817.0 8 17962.0 9 15958.0 10 16438.0 11 18125.0 12 19364.0 13 20558.0 14 21139.0 15 22431.0 16 24015.0 17 24485.0 18 23393.0 19 24245.0 20 25956.0 21 27402.0 22 29926.0 23 32831.0 24 34957.0 25 38839.0 26 42214.0 27 45854.0 28 50748.0 29 56029.0 30 62657.0 31 69111.0 32 77913.0 33 87403.0 34 96824.0 35 101280.0 36 91843.0 37 52696.0 38 9889.0 39 122.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.96404056537706 20.973444308027318 14.21313568141034 22.849379445185285 2 14.56133556883225 21.02988151706508 42.405086431581125 22.003696482521548 3 18.140323359486572 27.005482933043833 30.849700510621687 24.00449319684791 4 11.838986849970114 17.735953377166766 40.82023311416617 29.604826658696954 5 12.139822237590781 38.530287751041875 36.46593747726335 12.863952534103987 6 28.38653410818664 39.61585352119021 18.7793526370916 13.218259733531552 7 25.133728063600984 33.54874169317633 24.116090116198375 17.201440127024313 8 23.69492253542471 37.548806569828805 21.36452824818182 17.391742646564662 9 24.907634199791897 17.229676688358477 22.210355834432253 35.65233327741738 10-11 23.245777319884393 27.822350382523396 29.84972283915636 19.082149458435843 12-13 23.89596004450587 25.393262000868166 30.057054330091855 20.65372362453411 14-15 21.224583677445626 27.127860807685277 27.589980237536736 24.05757527733236 16-17 20.564659898091186 30.298879770275327 28.25306567285538 20.8833946587781 18-19 20.411151938860293 29.116281019722727 30.349281454503924 20.12328558691305 20-21 21.21347876464254 28.352817030852684 30.338978383997894 20.094725820506888 22-23 20.814634987321504 28.044966300431888 30.11658888894644 21.02380982330017 24-25 20.695610105495216 28.397706068373996 30.342195877890184 20.564487948240608 26-27 20.643147887847398 28.52055942431911 30.12118099046017 20.71511169737332 28-29 20.650536443613618 28.535433628945807 30.224305192561552 20.589724734879024 30-31 20.931317964759547 28.368285555575362 30.134637204190845 20.565759275474246 32-33 20.738881461203988 28.51200253693206 29.992728561543025 20.756387440320935 34-35 20.882857214720087 28.488342034072616 30.09220315015203 20.536597601055266 36-37 20.982030906796634 28.334687872945764 30.0170224319767 20.66625878828091 38-39 20.805413907538302 28.591315025919585 30.069777599851825 20.53349346669029 40-41 20.933686765001305 28.50939237424746 30.041948428561327 20.514972432189907 42-43 20.896087910469113 28.56636982624424 29.800734003691264 20.736808259595378 44-45 20.835820480175872 28.778550010783434 29.672079254760362 20.713550254280328 46-47 20.993725783694607 28.692891017343282 29.305242297740612 21.008140901221502 48-49 20.933724793483112 28.805562062792077 29.301407785998745 20.959305357726066 50-51 21.220887360336018 28.954356277357622 28.87062425522376 20.9541321070826 52-53 20.77581622310662 29.155372992342176 29.005876995817225 21.062933788733975 54-55 20.720727217780706 29.09685018819198 29.111239319761534 21.071183274265774 56-57 20.710193501373226 29.190084815506946 29.130135295968095 20.969586387151736 58-59 20.74173983475296 29.01483304730866 29.192080877632172 21.051346240306213 60-61 20.77152779467641 29.20107904040492 29.140243966029693 20.887149198888977 62-63 20.67059243100424 29.108071615710827 29.098878194453697 21.122457758831235 64-65 20.521899213595173 29.061044206772408 29.184874893234742 21.23218168639768 66-67 20.540751819531522 29.11654662063513 28.919263705493158 21.42343785434019 68-69 20.619013606929588 29.043607005941013 29.011538437511064 21.325840949618335 70-71 20.707404753593366 28.839507533769783 29.03728472634754 21.415802986289307 72-73 20.691674206423603 28.99658061834158 28.89815622376368 21.413588951471134 74-75 20.514413783718645 29.226133190804955 28.716375625679376 21.543077399797028 76-77 20.561519739989393 29.139445779300903 28.825134628817437 21.473899851892263 78-79 20.43555885772191 29.133518446014754 28.822271465444242 21.608651230819092 80-81 20.54458267436845 29.1759372806387 28.57912230106308 21.70035774392977 82-83 20.643375781470493 29.081632129448288 28.771934625877684 21.503057463203536 84-85 20.682857264078244 29.110317452225615 28.645984247928542 21.560841035767602 86-87 20.54215074780744 29.18815948349469 28.745101315251354 21.524588453446516 88-89 20.652861267806998 29.085481852935974 28.63750332628684 21.62415355297019 90-91 20.610374188044105 29.056928885927256 28.80356209230332 21.529134833725315 92-93 20.66851261843721 28.95169029319517 28.53712234046477 21.84267474790285 94-95 20.39315530936559 29.00808166934819 28.66655602693382 21.932206994352406 96-97 20.493367126586836 28.874120130703833 28.615541629668545 22.016971113040785 98-99 20.313447422297994 28.842623246366923 28.388768447477418 22.45516088385766 100-101 20.482313930588308 29.043018611494613 28.309599684471237 22.16506777344584 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 117826.0 1 107074.0 2 71341.0 3 35989.5 4 21974.5 5 17246.5 6 16172.0 7 16550.0 8 17104.0 9 16742.0 10 15009.5 11 12498.0 12 9844.0 13 7489.0 14 5754.5 15 4838.0 16 4645.0 17 4955.0 18 5713.0 19 6666.0 20 7698.5 21 8772.0 22 9945.5 23 11378.5 24 12861.0 25 14499.0 26 16568.5 27 19131.0 28 22227.0 29 25789.0 30 29686.5 31 33857.0 32 38417.5 33 42627.5 34 46299.0 35 49432.0 36 52867.5 37 55827.0 38 56839.0 39 57112.0 40 56992.0 41 56211.0 42 55311.0 43 55092.0 44 55026.0 45 54796.5 46 53791.0 47 52213.5 48 50449.5 49 48301.5 50 45215.0 51 41106.0 52 37460.0 53 33823.0 54 29835.0 55 25657.5 56 21178.5 57 16983.0 58 13398.0 59 10359.5 60 7854.5 61 5808.5 62 4114.5 63 2842.5 64 1861.5 65 1138.5 66 718.0 67 431.0 68 262.0 69 179.5 70 106.5 71 63.0 72 45.5 73 35.0 74 30.5 75 24.5 76 15.5 77 9.0 78 6.5 79 7.5 80 5.0 81 2.0 82 1.0 83 0.0 84 1.0 85 1.0 86 0.5 87 0.5 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 22.655534342639342 2 29.72682879253995 3 23.445521752968745 4 29.820844949895626 5 32.97623273639453 6 25.507742306589154 7 24.44184133239719 8 20.41786590139367 9 12.164212091641897 10-11 15.84507836165441 12-13 15.92637900414185 14-15 20.096831922916188 16-17 23.453413237453983 18-19 27.458407157602643 20-21 29.539111088505756 22-23 29.1338925363336 24-25 27.598985695176857 26-27 29.10413560978628 28-29 26.018643828728045 30-31 27.929431776412482 32-33 26.915441563950647 34-35 24.949832705772423 36-37 27.96592661496215 38-39 28.092740935410955 40-41 25.088104098380853 42-43 25.962564475525653 44-45 24.630293128011413 46-47 27.97733125200892 48-49 23.337374332697642 50-51 28.892114230942067 52-53 22.502685988656186 54-55 23.656573082225073 56-57 25.741838867946885 58-59 25.066422179213777 60-61 24.5817906486171 62-63 24.427998462602492 64-65 22.95472909661117 66-67 23.466810408789385 68-69 24.45867300362484 70-71 22.942249539750797 72-73 22.805236589850974 74-75 20.044711420734625 76-77 22.34273267688068 78-79 21.451860109411104 80-81 22.500247755908255 82-83 23.361494484612656 84-85 20.285808069658998 86-87 21.854719081504616 88-89 20.68809025551106 90-91 21.823362884015104 92-93 21.809231621099677 94-95 20.85425712816033 96-97 19.748951166655043 98-99 19.144374315394057 100-101 20.831028373163917 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1907119.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.14719921837926 #Duplication Level Percentage of deduplicated Percentage of total 1 88.40542083105979 67.31825192007365 2 7.778039303848364 11.845518167970505 3 1.8649879786384376 4.260408334477906 4 0.7314479232589965 2.227908429210904 5 0.34097980032146097 1.2982328392260731 6 0.2115298844129016 0.9664444949417973 7 0.1402751426895042 0.7477091465034976 8 0.10459416649872193 0.6371642266766806 9 0.07381240488598084 0.5058547109676409 >10 0.331325875722127 4.212236772602675 >50 0.013030048468211737 0.6751765607531027 >100 0.004276398921317552 0.48645535060413925 >500 7.006031852142979E-5 0.035690461142437076 >1k 1.4012063704285958E-4 0.15572564435691152 >5k 0.0 0.0 >10k+ 7.006031852142979E-5 4.627222940492196 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 86735 4.547959513800659 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 7.865266928807274E-5 0.0 12-13 0.0 0.0 0.0 1.0487022571743032E-4 0.0 14-15 0.0 0.0 0.0 1.0487022571743032E-4 0.0 16-17 0.0 0.0 0.0 2.621755642935758E-4 0.0 18-19 0.0 0.0 0.0 2.621755642935758E-4 0.0 20-21 0.0 0.0 0.0 3.1461067715229094E-4 0.0 22-23 0.0 0.0 0.0 4.1948090286972133E-4 0.0 24-25 0.0 0.0 0.0 4.98133572157794E-4 0.0 26-27 0.0 0.0 0.0 6.030037978752244E-4 0.0 28-29 0.0 0.0 0.0 8.389618057394427E-4 0.0 30-31 0.0 0.0 0.0 0.0020187518450605338 0.0 32-33 0.0 0.0 0.0 0.005689209745170594 0.0 34-35 0.0 0.0 0.0 0.009988888999585239 0.0 36-37 0.0 0.0 0.0 0.01756576280766958 0.0 38-39 0.0 0.0 0.0 0.02980936166017957 0.0 40-41 0.0 0.0 0.0 0.04265596431056479 0.0 42-43 0.0 0.0 0.0 0.05626287609740137 0.0 44-45 0.0 0.0 0.0 0.07233423818859756 0.0 46-47 0.0 0.0 0.0 0.09246932152634418 0.0 48-49 0.0 0.0 0.0 0.11538346584560272 0.0 50-51 0.0 0.0 0.0 0.14207293829068873 0.0 52-53 0.0 0.0 0.0 0.17154147171728665 0.0 54-55 0.0 0.0 0.0 0.20253062341678732 0.0 56-57 0.0 0.0 0.0 0.23768836658855583 0.0 58-59 0.0 0.0 0.0 0.2792431935290876 2.621755642935758E-5 60-61 0.0 0.0 0.0 0.3256220508526212 5.243511285871516E-5 62-63 0.0 0.0 0.0 0.37166007994257305 5.243511285871516E-5 64-65 0.0 0.0 0.0 0.4210277386990534 5.243511285871516E-5 66-67 0.0 0.0 0.0 0.47582243163641075 5.243511285871516E-5 68-69 0.0 0.0 0.0 0.5398719219933312 5.243511285871516E-5 70-71 0.0 0.0 0.0 0.6130189044312389 5.243511285871516E-5 72-73 0.0 0.0 0.0 0.6859561464177117 5.243511285871516E-5 74-75 0.0 0.0 0.0 0.7594439570892011 5.243511285871516E-5 76-77 0.0 0.0 0.0 0.8417670842773839 5.243511285871516E-5 78-79 0.0 0.0 0.0 0.9339480126830051 5.243511285871516E-5 80-81 0.0 0.0 0.0 1.041204036035507 5.243511285871516E-5 82-83 0.0 0.0 0.0 1.1555650171803649 5.243511285871516E-5 84-85 0.0 0.0 0.0 1.2696113876480704 5.243511285871516E-5 86-87 0.0 0.0 0.0 1.383972368792928 5.243511285871516E-5 88-89 0.0 0.0 0.0 1.5132773571025195 5.243511285871516E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1595 0.0 56.296062 1 GTATCAA 3085 0.0 44.241222 1 ATCAACG 3535 0.0 37.230995 3 TCAACGC 3590 0.0 36.798145 4 CAACGCA 3735 0.0 35.356937 5 TATCAAC 4000 0.0 33.470207 2 AACGCAG 4095 0.0 33.357643 6 ACGCAGA 4545 0.0 29.188549 7 CGCAGAG 4725 0.0 28.228222 8 GCAGAGT 5155 0.0 24.912973 9 GTGGTAT 820 0.0 24.40346 1 TGGTATC 915 0.0 22.42419 2 ACATGGG 3520 0.0 22.061325 3 GTACATG 3590 0.0 22.01035 1 AGAGTAC 4680 0.0 21.034864 10-11 GAGTACT 3380 0.0 20.894045 12-13 TACATGG 3685 0.0 20.601654 2 CAGAGTA 5025 0.0 20.501875 10-11 GAGTACA 2675 0.0 20.332123 1 CGTACAC 150 5.1632046E-4 20.170355 3 >>END_MODULE