FastQCFastQC Report
Sat 18 Jun 2016
SRR1931007_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931007_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1907119
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42710.22395036701957244No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38050.19951560442741118No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21700.11378419490341189No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26250.057.5848351
GTATCAA47950.040.149181
CAACGCA58900.032.6208465
ATCAACG58950.032.574193
TCAACGC59150.032.543554
AACGCAG61850.031.1408486
TATCAAC61450.031.0185412
ACGCAGA70250.027.1471237
CGCAGAG71200.026.7849088
CCGTACC950.001235933524.9683889
GTACATG53300.024.792931
ACATGGG51400.024.1353423
TACATGG54650.023.7449572
GCAGAGT82350.022.5822149
GTGGTAT14700.021.9888171
CAGAGTA81150.020.40238810-11
GAGTACT59000.020.10166712-13
TGGTATC14500.019.6689952
AGAGTAC77350.019.5340910-11
CGTATAC1759.8916345E-519.0123623
Produced by FastQC (version 0.11.5)