FastQCFastQC Report
Sat 18 Jun 2016
SRR1931006_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931006_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2348283
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1052014.479911492780044No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19100.055.8288961
GTATCAA39550.049.6456761
ATCAACG47800.040.901923
TCAACGC48950.039.834234
CAACGCA49100.039.8062325
TATCAAC49400.039.632
AACGCAG53550.036.4400066
ACGCAGA60100.031.998637
CGCAGAG62250.031.089428
GCAGAGT67250.026.2211599
GTACATG43800.025.2818451
TACATGG45150.023.9503772
ACATGGG45250.023.7192463
GTGGTAT9700.022.1976341
GAGTACT45750.021.83783712-13
TAACGCC1403.2594663E-421.5934164
GAGTACA32800.021.2566181
CAGAGTA65000.021.18100510-11
GTACTTT47900.021.1608514-15
GTATAGG6300.021.1573811
Produced by FastQC (version 0.11.5)