##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931006_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2348283 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.854801572042213 30.0 2.0 31.0 2.0 33.0 2 20.46165687866411 28.0 2.0 31.0 2.0 33.0 3 21.196045791755083 28.0 2.0 31.0 2.0 34.0 4 22.705493332788254 32.0 2.0 35.0 2.0 37.0 5 22.02836029558618 32.0 2.0 35.0 2.0 37.0 6 23.087701099058332 32.0 2.0 35.0 2.0 37.0 7 23.32052738106949 32.0 2.0 35.0 2.0 37.0 8 23.81605794531579 32.0 2.0 35.0 2.0 37.0 9 25.26133902941 33.0 10.0 37.0 2.0 39.0 10-11 25.699632029018648 33.5 9.0 37.0 2.0 39.0 12-13 25.668620647511396 34.0 5.0 37.0 2.0 39.0 14-15 25.67631456685587 33.5 2.0 38.0 2.0 40.0 16-17 24.46764699995699 32.5 2.0 38.0 2.0 40.0 18-19 23.343210124163058 31.5 2.0 38.0 2.0 40.0 20-21 22.99905548011036 31.5 2.0 38.0 2.0 40.0 22-23 22.624446457262604 30.0 2.0 38.0 2.0 40.0 24-25 22.479298491706494 30.0 2.0 38.0 2.0 40.0 26-27 22.201492324391907 30.0 2.0 38.0 2.0 40.0 28-29 22.03352917855301 29.0 2.0 38.0 2.0 40.0 30-31 22.075453001192784 30.0 2.0 37.0 2.0 40.0 32-33 21.976059103608893 29.5 2.0 37.0 2.0 40.0 34-35 21.67790274851881 29.0 2.0 37.0 2.0 40.0 36-37 21.196427772972847 27.0 2.0 37.0 2.0 39.5 38-39 21.11785674895232 27.0 2.0 37.0 2.0 39.0 40-41 21.025682168631292 27.0 2.0 37.0 2.0 39.0 42-43 20.90790888491719 26.5 2.0 37.0 2.0 39.0 44-45 20.518587836304228 25.0 2.0 36.0 2.0 39.0 46-47 20.179427479566986 24.0 2.0 36.0 2.0 39.0 48-49 19.961647723038492 24.0 2.0 36.0 2.0 39.0 50-51 17.472716022728093 12.0 2.0 33.5 2.0 38.5 52-53 19.502485220052268 23.0 2.0 35.0 2.0 38.5 54-55 20.368178792760496 26.0 2.0 37.0 2.0 39.0 56-57 20.37530208241511 26.0 2.0 37.0 2.0 39.0 58-59 20.103701300056255 26.0 2.0 36.5 2.0 39.0 60-61 19.820242279146083 25.0 2.0 36.0 2.0 39.0 62-63 19.387632580911244 23.5 2.0 36.0 2.0 39.0 64-65 19.054456596585673 22.5 2.0 35.0 2.0 39.0 66-67 18.70763361996829 22.0 2.0 35.0 2.0 38.5 68-69 18.26919881462328 20.0 2.0 34.0 2.0 38.0 70-71 17.92926619151099 18.5 2.0 34.0 2.0 38.0 72-73 17.536467282691227 16.5 2.0 34.0 2.0 37.0 74-75 17.091986357691983 12.5 2.0 33.0 2.0 36.5 76-77 16.690503018588473 8.0 2.0 32.5 2.0 36.0 78-79 16.326009897444216 7.0 2.0 32.0 2.0 35.5 80-81 15.905151976997661 4.5 2.0 32.0 2.0 35.0 82-83 15.450224696086458 2.0 2.0 31.5 2.0 35.0 84-85 15.06824283955554 2.0 2.0 31.0 2.0 34.5 86-87 14.588452499123829 2.0 2.0 31.0 2.0 34.0 88-89 14.170040834090269 2.0 2.0 31.0 2.0 34.0 90-91 13.686842684633836 2.0 2.0 30.0 2.0 34.0 92-93 13.14091487269635 2.0 2.0 29.5 2.0 34.0 94-95 12.564334026179978 2.0 2.0 29.0 2.0 34.0 96-97 11.902412741564795 2.0 2.0 29.0 2.0 34.0 98-99 11.18542803401464 2.0 2.0 28.0 2.0 34.0 100-101 10.13222426768835 2.0 2.0 23.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 8.014257532651126 1101 2 9.730844421423399 1101 3 8.795490336876089 1101 4 11.021450455829118 1101 5 11.99710555553774 1101 6 10.93608611514674 1101 7 10.528626680812042 1101 8 9.876853079600767 1101 9 9.833941440650797 1101 10-11 9.069520152603438 1101 12-13 8.86153100084908 1101 14-15 9.685454769578598 1101 16-17 10.820800652510414 1101 18-19 11.697411260652519 1101 20-21 11.889433792239153 1101 22-23 12.04568649416316 1101 24-25 12.033852158733875 1101 26-27 11.97703408509749 1101 28-29 11.861491826347969 1101 30-31 11.585795018448625 1101 32-33 11.457685622619913 1101 34-35 11.305949813698277 1101 36-37 11.786941918711722 1101 38-39 11.52794897341747 1101 40-41 11.20351491152951 1101 42-43 11.086918690551045 1101 44-45 10.952123508702268 1101 46-47 10.684399001679392 1101 48-49 10.955020578075686 1101 50-51 12.943170640851587 1101 52-53 11.976543606786077 1101 54-55 11.427278515337512 1101 56-57 11.546472206241962 1101 58-59 11.273102576361321 1101 60-61 10.759484561172563 1101 62-63 11.06104537367207 1101 64-65 10.873723414081066 1101 66-67 10.680352742665718 1101 68-69 10.734390536771446 1101 70-71 10.320865108863085 1101 72-73 10.142994027347857 1101 74-75 9.941941909027484 1101 76-77 9.708664811737325 1101 78-79 9.50444972430438 1101 80-81 9.228099931147554 1101 82-83 8.726284025866876 1101 84-85 8.643893707777814 1101 86-87 8.574700708988694 1101 88-89 8.454429065268458 1101 90-91 8.151484611578363 1101 92-93 7.945721996511866 1101 94-95 7.668806024379267 1101 96-97 7.481966290933384 1101 98-99 7.28309770816751 1101 100-101 6.461183133139054 1102 1 8.034381462110947 1102 2 9.429419538702849 1102 3 8.842525935293441 1102 4 10.90197705864827 1102 5 11.526078309977105 1102 6 10.75064267917882 1102 7 10.442329595129873 1102 8 9.80112992588261 1102 9 9.504139243643579 1102 10-11 8.610000692283677 1102 12-13 9.005654994304802 1102 14-15 9.35806528772283 1102 16-17 10.49144538342155 1102 18-19 11.667033030946175 1102 20-21 11.829651879151157 1102 22-23 11.961172250900812 1102 24-25 11.594652441428831 1102 26-27 11.777417643158476 1102 28-29 11.74782153602916 1102 30-31 11.612056989856367 1102 32-33 11.649279621473553 1102 34-35 11.549198245798621 1102 36-37 11.76022049844784 1102 38-39 11.485867144915364 1102 40-41 11.144818841991004 1102 42-43 10.870446631719329 1102 44-45 10.978335048555024 1102 46-47 10.833428420318338 1102 48-49 10.470893996959482 1102 50-51 12.419430247193304 1102 52-53 11.443076879091432 1102 54-55 11.215200610062581 1102 56-57 10.975314325563716 1102 58-59 11.009417539160225 1102 60-61 10.828613354321106 1102 62-63 11.00516790635172 1102 64-65 10.925604963227137 1102 66-67 10.669227541635106 1102 68-69 10.465178896751187 1102 70-71 10.165960587969817 1102 72-73 10.1453990908151 1102 74-75 9.634110132000345 1102 76-77 9.648558745482081 1102 78-79 9.237321081407089 1102 80-81 8.871938725464716 1102 82-83 8.775654431378316 1102 84-85 8.863345401999815 1102 86-87 8.659172867987913 1102 88-89 8.415097669434315 1102 90-91 8.130181974947108 1102 92-93 7.780025470654737 1102 94-95 7.5286536028298965 1102 96-97 7.309864609788802 1102 98-99 6.870485854456586 1102 100-101 6.1846648303104335 1103 1 8.044483061597884 1103 2 9.554566096168092 1103 3 8.889788621711457 1103 4 11.123179928402578 1103 5 11.59320351129043 1103 6 10.71668275635719 1103 7 10.338277388160957 1103 8 9.793296929868152 1103 9 9.620965477994524 1103 10-11 8.912856004730855 1103 12-13 8.741741104045115 1103 14-15 9.435858298274965 1103 16-17 10.491048403480669 1103 18-19 11.527277526100622 1103 20-21 11.923904756057944 1103 22-23 12.1578071101201 1103 24-25 12.128476843512015 1103 26-27 11.825613574661634 1103 28-29 11.728889613982329 1103 30-31 11.336128125239537 1103 32-33 11.5200257760726 1103 34-35 11.273607156355617 1103 36-37 11.652779246345734 1103 38-39 11.014616877125018 1103 40-41 10.868481019590245 1103 42-43 10.575330374774747 1103 44-45 10.582590707743638 1103 46-47 10.66968976590238 1103 48-49 10.91070507899676 1103 50-51 12.67240400339697 1103 52-53 11.480512943076384 1103 54-55 11.177241627672746 1103 56-57 10.743528347032118 1103 58-59 10.661709653427476 1103 60-61 10.564854063792701 1103 62-63 10.45930919425047 1103 64-65 10.2903628807525 1103 66-67 10.162079934910814 1103 68-69 9.86803901744678 1103 70-71 9.771494881903344 1103 72-73 9.703531156459153 1103 74-75 9.580016909474608 1103 76-77 9.328838116693234 1103 78-79 8.827073711570307 1103 80-81 8.525842629625544 1103 82-83 8.50059746817904 1103 84-85 8.265994113765686 1103 86-87 8.020188565122332 1103 88-89 7.805808663930671 1103 90-91 7.864591357773344 1103 92-93 7.6562991431503935 1103 94-95 7.440757538338451 1103 96-97 6.959236782590066 1103 98-99 6.574907081658473 1103 100-101 5.906447427668054 1104 1 7.930049752528941 1104 2 9.476273495417534 1104 3 8.808055340446494 1104 4 10.851220779908978 1104 5 11.676967425892347 1104 6 10.735926220147594 1104 7 10.383789245210455 1104 8 9.807302957427002 1104 9 9.515477905182749 1104 10-11 8.868858232357283 1104 12-13 8.687024822904373 1104 14-15 9.44206548308145 1104 16-17 10.600968982676775 1104 18-19 11.564896178882837 1104 20-21 11.733741079766403 1104 22-23 12.113208847860587 1104 24-25 11.785345543061478 1104 26-27 11.802245081946655 1104 28-29 11.665840408876786 1104 30-31 11.472948517647804 1104 32-33 11.359298592000492 1104 34-35 11.345273527780734 1104 36-37 11.461746302847022 1104 38-39 11.223360178573916 1104 40-41 10.671766206221776 1104 42-43 10.719394605442258 1104 44-45 10.49463428043434 1104 46-47 10.486430854941776 1104 48-49 10.52090220938122 1104 50-51 12.255031279382653 1104 52-53 11.305667341174797 1104 54-55 10.956937676083761 1104 56-57 10.708346057290829 1104 58-59 10.702472339262187 1104 60-61 10.497208518392242 1104 62-63 10.51198036942927 1104 64-65 10.405244262291088 1104 66-67 10.28151969711649 1104 68-69 9.805441523642305 1104 70-71 9.4872605490988 1104 72-73 9.603246285578951 1104 74-75 9.438335782588062 1104 76-77 9.122482550265293 1104 78-79 8.762848750410697 1104 80-81 8.584416237478937 1104 82-83 8.319928081982086 1104 84-85 7.978010378680551 1104 86-87 7.625556883504203 1104 88-89 7.262815870636308 1104 90-91 6.977945670623859 1104 92-93 6.649656704484963 1104 94-95 6.535562825295871 1104 96-97 5.995768665286709 1104 98-99 5.825089560784399 1104 100-101 5.149537576243196 1105 1 8.075503689739104 1105 2 9.335295985873561 1105 3 8.767426948413739 1105 4 10.94006987111215 1105 5 10.965422805822751 1105 6 10.568702375054567 1105 7 10.151725486199954 1105 8 9.770928511438722 1105 9 9.57953458635457 1105 10-11 9.065160912595012 1105 12-13 8.669212164771903 1105 14-15 9.55164612633591 1105 16-17 10.64536023723278 1105 18-19 11.308394762470638 1105 20-21 11.566126024288906 1105 22-23 11.996128462814728 1105 24-25 11.967271850652548 1105 26-27 12.108235423970743 1105 28-29 11.855496816055176 1105 30-31 11.30325723769317 1105 32-33 11.370708164101757 1105 34-35 11.500572033158939 1105 36-37 11.491437855625836 1105 38-39 11.282733702806222 1105 40-41 11.140010283825962 1105 42-43 10.686962387635024 1105 44-45 10.762134733517353 1105 46-47 10.706962433366058 1105 48-49 10.575827481539577 1105 50-51 12.122086899138498 1105 52-53 10.95647975636383 1105 54-55 10.809408163977835 1105 56-57 10.742173007512758 1105 58-59 10.813170506078578 1105 60-61 10.533232271634468 1105 62-63 10.505186722594974 1105 64-65 10.269548417728114 1105 66-67 10.10130795957075 1105 68-69 10.041824511625702 1105 70-71 9.811077840045652 1105 72-73 9.571900492625222 1105 74-75 9.587057763707328 1105 76-77 9.147289657075309 1105 78-79 8.888641645679343 1105 80-81 8.466193319988115 1105 82-83 8.579395974148978 1105 84-85 8.092978162692148 1105 86-87 7.852769162800811 1105 88-89 7.805249449316529 1105 90-91 7.241355060192751 1105 92-93 7.124905705266409 1105 94-95 6.811784639147936 1105 96-97 6.184055511328777 1105 98-99 6.145604867195182 1105 100-101 5.433214635562317 1106 1 7.9574240605768125 1106 2 9.264889520481788 1106 3 8.808946430366575 1106 4 10.782944689504554 1106 5 11.401027705145498 1106 6 10.731049884376173 1106 7 9.746569831305695 1106 8 9.386099675992792 1106 9 9.090508977666573 1106 10-11 8.562345067053926 1106 12-13 8.356790786096756 1106 14-15 8.924753647714134 1106 16-17 10.22361802417278 1106 18-19 11.472772549513909 1106 20-21 11.475332553190896 1106 22-23 11.5806572383003 1106 24-25 11.4094078464237 1106 26-27 11.637892726968051 1106 28-29 11.560678304246732 1106 30-31 11.168041306180935 1106 32-33 11.435352723102682 1106 34-35 11.20306942401205 1106 36-37 11.424145585348551 1106 38-39 11.080925952881575 1106 40-41 11.22005526377571 1106 42-43 10.855370879028552 1106 44-45 10.446626509783247 1106 46-47 10.214499753686685 1106 48-49 10.252554423748503 1106 50-51 12.120691562234224 1106 52-53 11.29872713705143 1106 54-55 10.863614360483872 1106 56-57 10.836988469668995 1106 58-59 10.47338656667662 1106 60-61 10.466423024314452 1106 62-63 10.516779596283865 1106 64-65 10.537203241632486 1106 66-67 10.315434206781688 1106 68-69 10.008577816383738 1106 70-71 9.387240725681433 1106 72-73 9.257199539261986 1106 74-75 8.894796393345302 1106 76-77 8.861749671778181 1106 78-79 8.589441880873686 1106 80-81 8.24749396599804 1106 82-83 8.277812075150882 1106 84-85 8.057076817811659 1106 86-87 7.778818727233023 1106 88-89 7.664731802318451 1106 90-91 7.391713644431244 1106 92-93 7.058748906026468 1106 94-95 6.630873783870554 1106 96-97 6.133324627484706 1106 98-99 5.835984327835952 1106 100-101 5.273783013282882 1107 1 7.434294323292491 1107 2 8.46572236598482 1107 3 8.359945175624777 1107 4 10.192176231180461 1107 5 11.06443844231313 1107 6 9.821944426835227 1107 7 9.822270553315942 1107 8 8.457550455748127 1107 9 5.836957554360481 1107 10-11 7.757181733122941 1107 12-13 7.549993093588906 1107 14-15 8.333546423020962 1107 16-17 9.445308604556274 1107 18-19 10.851327203174197 1107 20-21 11.032566082678287 1107 22-23 10.529340995373172 1107 24-25 10.726362414561859 1107 26-27 11.002808865368142 1107 28-29 11.09024675942474 1107 30-31 10.740792895394815 1107 32-33 11.019622190340627 1107 34-35 11.141630655556298 1107 36-37 11.236097719284412 1107 38-39 10.997566041023298 1107 40-41 10.56541664369955 1107 42-43 10.346521393951019 1107 44-45 9.567823138914672 1107 46-47 9.885127944815142 1107 48-49 10.06779983355565 1107 50-51 11.879189961984974 1107 52-53 10.700410645166322 1107 54-55 10.167685553798378 1107 56-57 9.833496316339769 1107 58-59 9.923072897278374 1107 60-61 10.11514965480864 1107 62-63 10.06898179896006 1107 64-65 10.24040865186981 1107 66-67 10.192798725726973 1107 68-69 9.991055733200955 1107 70-71 9.554865530114586 1107 72-73 9.353062727622376 1107 74-75 9.30535169805237 1107 76-77 9.115621642528591 1107 78-79 8.887344662702226 1107 80-81 8.491240441701684 1107 82-83 8.578268963606307 1107 84-85 8.203773903045015 1107 86-87 7.976531893289923 1107 88-89 7.966361249151797 1107 90-91 7.757232595013166 1107 92-93 7.270938232229961 1107 94-95 6.970755841030728 1107 96-97 6.608303949205029 1107 98-99 6.311367664537414 1107 100-101 5.716933434464888 1108 1 6.989200186173338 1108 2 8.160334519306396 1108 3 8.152675719215125 1108 4 9.524488505873766 1108 5 11.25899291129603 1108 6 9.792229630179396 1108 7 8.915500903789155 1108 8 6.161740312786431 1108 9 7.260815926283534 1108 10-11 6.23350809671032 1108 12-13 5.252125084093407 1108 14-15 7.426643767810049 1108 16-17 8.7730903529079 1108 18-19 8.755853231270105 1108 20-21 9.180961835104547 1108 22-23 9.924322085751182 1108 24-25 9.558449857870617 1108 26-27 8.123869522594209 1108 28-29 9.892705164000528 1108 30-31 9.977044646353157 1108 32-33 10.411618015062437 1108 34-35 10.116008210798555 1108 36-37 9.950380474806213 1108 38-39 9.411239041532827 1108 40-41 9.69871269121397 1108 42-43 9.08716055260431 1108 44-45 8.667878687395646 1108 46-47 9.368140837467099 1108 48-49 9.848749018426592 1108 50-51 11.501281008744566 1108 52-53 10.677784873486598 1108 54-55 10.338893803645835 1108 56-57 10.199486617280503 1108 58-59 10.118642464995336 1108 60-61 9.988907681871574 1108 62-63 10.049333121662755 1108 64-65 9.811426972189242 1108 66-67 9.166800806893772 1108 68-69 9.557963660097016 1108 70-71 9.486037192072388 1108 72-73 9.304379491985653 1108 74-75 9.093992155048252 1108 76-77 9.115899223126068 1108 78-79 8.825176317310735 1108 80-81 8.619587043511629 1108 82-83 8.446580958049195 1108 84-85 8.477466349568076 1108 86-87 8.045954145399769 1108 88-89 7.988853367917779 1108 90-91 7.972593149637161 1108 92-93 7.5555208023042955 1108 94-95 7.400689352751327 1108 96-97 7.009380971631698 1108 98-99 6.627871517899777 1108 100-101 5.995290060243363 1109 1 6.7798783852285 1109 2 8.329123075136835 1109 3 8.096859981723995 1109 4 9.250041792029148 1109 5 10.486234663145108 1109 6 9.149076506398004 1109 7 7.9083009920080904 1109 8 8.564124141871833 1109 9 7.66237749734772 1109 10-11 6.050550173307371 1109 12-13 6.591315467590075 1109 14-15 8.102198412409194 1109 16-17 7.0342077767871025 1109 18-19 6.123742417418455 1109 20-21 8.58157625122944 1109 22-23 9.755539443146542 1109 24-25 9.116556411003348 1109 26-27 9.845143391250275 1109 28-29 10.041924618193807 1109 30-31 7.482855988879162 1109 32-33 6.773484980267096 1109 34-35 8.515092060340521 1109 36-37 6.642084059179954 1109 38-39 7.033865520638365 1109 40-41 8.098505488585303 1109 42-43 6.87749242840739 1109 44-45 7.427970246505531 1109 46-47 7.100276662665749 1109 48-49 7.650670097638248 1109 50-51 9.647099079562633 1109 52-53 9.73573296814935 1109 54-55 9.75449688875154 1109 56-57 8.800105348462061 1109 58-59 8.941652504693856 1109 60-61 8.994198820343343 1109 62-63 9.64430703954243 1109 64-65 9.64391439459813 1109 66-67 9.105770802866385 1109 68-69 8.791015795570619 1109 70-71 8.242889581974513 1109 72-73 7.661117932428283 1109 74-75 6.8694044826857095 1109 76-77 6.66126541162053 1109 78-79 7.5295592162616956 1109 80-81 7.5162256942733165 1109 82-83 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2313 60-61 -17.47504721140269 2313 62-63 -17.049740579505187 2313 64-65 -16.73267290698917 2313 66-67 -16.381729197133613 2313 68-69 -15.947674680619858 2313 70-71 -15.628340576755644 2313 72-73 -15.250191591381242 2313 74-75 -14.810357422076194 2313 76-77 -14.41591712806201 2313 78-79 -14.051988402785923 2313 80-81 -13.622861607313986 2313 82-83 -13.17484473667493 2313 84-85 -12.80928354974727 2313 86-87 -12.336043438168495 2313 88-89 -11.924960566838562 2313 90-91 -11.449476926883177 2313 92-93 -10.915482016866063 2313 94-95 -10.36672909267759 2313 96-97 -9.704070498364276 2313 98-99 -9.00181031858834 2313 100-101 -7.976316866860946 2314 1 -19.537581932231816 2314 2 -18.154607083593323 2314 3 -18.865044780180767 2314 4 -20.32972011273276 2314 5 -19.615352638442193 2314 6 -20.71441555709406 2314 7 -20.92622281751572 2314 8 -21.459685381937692 2314 9 -22.90309869312847 2314 10-11 -23.362545064787867 2314 12-13 -23.348962310187705 2314 14-15 -23.359904762193977 2314 16-17 -22.118371588292263 2314 18-19 -20.971694090518053 2314 20-21 -20.62854279638961 2314 22-23 -20.26033357272647 2314 24-25 -20.11915787471094 2314 26-27 -19.846126450019565 2314 28-29 -19.66045633418715 2314 30-31 -19.694723376200205 2314 32-33 -19.617983273701157 2314 34-35 -19.338280955532497 2314 36-37 -18.857915940820046 2314 38-39 -18.77744400317116 2314 40-41 -18.68026435268454 2314 42-43 -18.571317095402133 2314 44-45 -18.171831340796057 2314 46-47 -17.83504079765171 2314 48-49 -17.615004505536355 2314 50-51 -15.229488222024667 2314 52-53 -17.186290841374458 2314 54-55 -18.01732850807386 2314 56-57 -18.042156556299844 2314 58-59 -17.760133209591856 2314 60-61 -17.47504721140269 2314 62-63 -17.049740579505187 2314 64-65 -16.73267290698917 2314 66-67 -16.381729197133613 2314 68-69 -15.947674680619858 2314 70-71 -15.628340576755644 2314 72-73 -15.250191591381242 2314 74-75 -14.810357422076194 2314 76-77 -14.41591712806201 2314 78-79 -14.051988402785923 2314 80-81 -13.622861607313986 2314 82-83 -13.17484473667493 2314 84-85 -12.80928354974727 2314 86-87 -12.336043438168495 2314 88-89 -11.924960566838562 2314 90-91 -11.449476926883177 2314 92-93 -10.915482016866063 2314 94-95 -10.36672909267759 2314 96-97 -9.704070498364276 2314 98-99 -9.00181031858834 2314 100-101 -7.976316866860946 2315 1 -19.537581932231816 2315 2 -18.154607083593323 2315 3 -18.865044780180767 2315 4 -20.32972011273276 2315 5 -19.615352638442193 2315 6 -20.71441555709406 2315 7 -20.92622281751572 2315 8 -21.459685381937692 2315 9 -22.90309869312847 2315 10-11 -23.362545064787867 2315 12-13 -23.348962310187705 2315 14-15 -23.359904762193977 2315 16-17 -22.118371588292263 2315 18-19 -20.971694090518053 2315 20-21 -20.62854279638961 2315 22-23 -20.26033357272647 2315 24-25 -20.11915787471094 2315 26-27 -19.846126450019565 2315 28-29 -19.66045633418715 2315 30-31 -19.694723376200205 2315 32-33 -19.617983273701157 2315 34-35 -19.338280955532497 2315 36-37 -18.857915940820046 2315 38-39 -18.77744400317116 2315 40-41 -18.68026435268454 2315 42-43 -18.571317095402133 2315 44-45 -18.171831340796057 2315 46-47 -17.83504079765171 2315 48-49 -17.615004505536355 2315 50-51 -15.229488222024667 2315 52-53 -17.186290841374458 2315 54-55 -18.01732850807386 2315 56-57 -18.042156556299844 2315 58-59 -17.760133209591856 2315 60-61 -17.47504721140269 2315 62-63 -17.049740579505187 2315 64-65 -16.73267290698917 2315 66-67 -16.381729197133613 2315 68-69 -15.947674680619858 2315 70-71 -15.628340576755644 2315 72-73 -15.250191591381242 2315 74-75 -14.810357422076194 2315 76-77 -14.41591712806201 2315 78-79 -14.051988402785923 2315 80-81 -13.622861607313986 2315 82-83 -13.17484473667493 2315 84-85 -12.80928354974727 2315 86-87 -12.336043438168495 2315 88-89 -11.924960566838562 2315 90-91 -11.449476926883177 2315 92-93 -10.915482016866063 2315 94-95 -10.36672909267759 2315 96-97 -9.704070498364276 2315 98-99 -9.00181031858834 2315 100-101 -7.976316866860946 2316 1 -19.537581932231816 2316 2 -18.154607083593323 2316 3 -18.865044780180767 2316 4 -20.32972011273276 2316 5 -19.615352638442193 2316 6 -20.71441555709406 2316 7 -20.92622281751572 2316 8 -21.459685381937692 2316 9 -22.90309869312847 2316 10-11 -23.362545064787867 2316 12-13 -23.348962310187705 2316 14-15 -23.359904762193977 2316 16-17 -22.118371588292263 2316 18-19 -20.971694090518053 2316 20-21 -20.62854279638961 2316 22-23 -20.26033357272647 2316 24-25 -20.11915787471094 2316 26-27 -19.846126450019565 2316 28-29 -19.66045633418715 2316 30-31 -19.694723376200205 2316 32-33 -19.617983273701157 2316 34-35 -19.338280955532497 2316 36-37 -18.857915940820046 2316 38-39 -18.77744400317116 2316 40-41 -18.68026435268454 2316 42-43 -18.571317095402133 2316 44-45 -18.171831340796057 2316 46-47 -17.83504079765171 2316 48-49 -17.615004505536355 2316 50-51 -15.229488222024667 2316 52-53 -17.186290841374458 2316 54-55 -18.01732850807386 2316 56-57 -18.042156556299844 2316 58-59 -17.760133209591856 2316 60-61 -17.47504721140269 2316 62-63 -17.049740579505187 2316 64-65 -16.73267290698917 2316 66-67 -16.381729197133613 2316 68-69 -15.947674680619858 2316 70-71 -15.628340576755644 2316 72-73 -15.250191591381242 2316 74-75 -14.810357422076194 2316 76-77 -14.41591712806201 2316 78-79 -14.051988402785923 2316 80-81 -13.622861607313986 2316 82-83 -13.17484473667493 2316 84-85 -12.80928354974727 2316 86-87 -12.336043438168495 2316 88-89 -11.924960566838562 2316 90-91 -11.449476926883177 2316 92-93 -10.915482016866063 2316 94-95 -10.36672909267759 2316 96-97 -9.704070498364276 2316 98-99 -9.00181031858834 2316 100-101 -7.976316866860946 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 570187.0 3 20817.0 4 91481.0 5 45202.0 6 22272.0 7 23574.0 8 22704.0 9 21041.0 10 22288.0 11 23907.0 12 25481.0 13 26768.0 14 28018.0 15 28899.0 16 30954.0 17 32110.0 18 30649.0 19 31567.0 20 33523.0 21 35515.0 22 38174.0 23 41711.0 24 45041.0 25 48796.0 26 53388.0 27 58006.0 28 63866.0 29 69550.0 30 77065.0 31 86104.0 32 95381.0 33 106160.0 34 114656.0 35 117982.0 36 101381.0 37 54261.0 38 9691.0 39 113.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.968538463646574 20.578694949162763 14.275206226534024 23.177560360656635 2 14.550717335991429 20.95720331169011 41.98160225729118 22.51047709502728 3 18.235805490647735 26.676430525254986 30.53544336039159 24.55232062370569 4 11.975112698920382 17.556530502634168 40.21151792018442 30.25683887826103 5 12.413797891638128 38.41574947076356 35.92830660747129 13.24214603012703 6 28.944807744396673 39.22911113791918 18.481313888882568 13.34476722880158 7 25.7334338582845 33.215306270131634 23.703749827503223 17.347510044080643 8 24.283985886516508 36.789235803903594 21.12689343467184 17.79988487490806 9 25.335832839331147 16.9331699660855 21.87983223589096 35.851164958692394 10-11 23.566301170383376 27.49176504768719 29.499048072046406 19.442885709883026 12-13 24.269149427399924 25.132600195861265 29.629456645309087 20.968793731429724 14-15 21.5336851233398 26.861328287835523 27.18783239190476 24.417154196919917 16-17 20.901639684729055 29.90720923515809 27.976987425105428 21.214163655007432 18-19 20.709836307286245 28.720458158577404 30.134057807046098 20.43564772709025 20-21 21.41840612912609 28.024807623425108 30.1474706777473 20.4093155697015 22-23 21.133159184687987 27.799035171892122 29.86491928497084 21.202886358449057 24-25 21.05110830500811 28.060799115053314 30.035307148783126 20.85278543115545 26-27 20.892360623717636 28.259241229157396 29.798107104778232 21.050291042346736 28-29 20.940047315991777 28.299345252075085 29.90677145688574 20.853835975047403 30-31 21.160023809341936 28.073399994006532 29.829338961708206 20.937237234943325 32-33 20.95445720285918 28.27415929762671 29.683751748731073 21.087631750783036 34-35 21.173548192896963 28.211104378081746 29.819190865569517 20.796156563451774 36-37 21.245332406723097 28.00467773542573 29.727226172270722 21.02276368558045 38-39 21.135854037349393 28.214239227167866 29.80580248418744 20.84410425129531 40-41 21.23608182905617 28.066580885806207 29.8351321285536 20.86220515658402 42-43 21.14120125755389 28.252670241773238 29.441692198032403 21.164436302640468 44-45 21.10823982593787 28.43376706364653 29.460173387123994 20.99781972329161 46-47 21.319016059887915 28.36422602630975 29.075189207943495 21.24156870585883 48-49 21.325793323680266 28.522029842692554 28.88440697475772 21.26776985886946 50-51 21.55484551692071 28.68661067164224 28.481875137851485 21.27666867358557 52-53 21.060546835999805 28.82439040535517 28.783141233826182 21.331921524818846 54-55 21.02899246801056 28.80684736408132 28.862205959516597 21.301954208391518 56-57 21.03638733863078 28.88133704862566 28.80936372384617 21.272911888897386 58-59 21.110099062073658 28.647150516569052 28.923485135035044 21.319265286322242 60-61 21.036018104428877 28.950230238419177 28.846594154183187 21.167157502968756 62-63 20.976792335593682 28.809659466892178 28.84633502392323 21.36721317359091 64-65 20.830827785367013 28.705354711319615 28.88044506234207 21.583372440971306 66-67 20.86074975046619 28.795080480710794 28.64119322154034 21.702976547282677 68-69 20.904141804693165 28.721133369387402 28.743798064300414 21.630926761619023 70-71 20.925537878566992 28.534919402797865 28.812543907472065 21.726998811163075 72-73 20.925184328843148 28.717221696184996 28.63362616021407 21.723967814757785 74-75 20.850550055195207 28.932237370431867 28.40941499447252 21.807797579900402 76-77 20.90174751451713 28.834166945295493 28.50155918417479 21.762526356012582 78-79 20.700496422224713 28.860819616166715 28.53743676654932 21.901247195059256 80-81 20.816783435579634 28.986860454653485 28.321582598245744 21.874773511521138 82-83 20.975033836140454 28.75929543409107 28.4805602905415 21.785110439226973 84-85 20.96978641640355 28.9232608441778 28.24733442808815 21.8596183113305 86-87 20.872838976454194 29.10728050195469 28.255293019360316 21.764587502230796 88-89 20.943720312807763 28.898130620572392 28.234692947607293 21.923456119012553 90-91 20.91859170707611 28.790286767444922 28.40668847502531 21.88443305045365 92-93 20.86505904105753 28.79096285947331 28.226317774013477 22.11766032545568 94-95 20.640316866226026 28.721787874712902 28.392460015354704 22.245435243706364 96-97 20.78985122420995 28.601755929498612 28.285392706260946 22.323000140030487 98-99 20.556084852976387 28.665874162881817 28.051093678825122 22.72694730531667 100-101 20.781892342924227 28.740491048571435 28.039383286524977 22.43823332197936 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 142282.0 1 129166.0 2 85695.0 3 43273.5 4 26881.5 5 21096.5 6 19581.0 7 19874.0 8 20552.5 9 20415.0 10 18518.5 11 15569.5 12 12427.0 13 9449.0 14 7327.0 15 5996.0 16 5494.0 17 5956.0 18 6792.0 19 8079.0 20 9486.0 21 10567.0 22 11993.5 23 13619.0 24 15365.0 25 17266.0 26 19494.0 27 22362.5 28 25467.5 29 29359.0 30 34089.0 31 38823.5 32 44132.5 33 48729.5 34 52656.0 35 56524.5 36 60701.0 37 65030.5 38 67378.0 39 67994.5 40 67824.5 41 67787.5 42 67780.5 43 67820.0 44 67919.5 45 68307.0 46 67626.0 47 65926.5 48 64624.5 49 62154.0 50 58196.0 51 54040.5 52 50109.5 53 46002.5 54 41244.0 55 35809.5 56 30345.5 57 24866.5 58 20048.5 59 15982.0 60 12120.0 61 8852.0 62 6361.5 63 4443.5 64 2919.5 65 1829.0 66 1116.5 67 686.0 68 418.5 69 273.5 70 168.0 71 106.5 72 69.0 73 52.5 74 40.0 75 26.0 76 18.0 77 11.5 78 8.5 79 7.0 80 5.0 81 2.5 82 3.0 83 2.0 84 1.5 85 1.0 86 1.5 87 1.5 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 22.307064352976198 2 29.338414492631426 3 23.09359647027211 4 29.434825359635102 5 32.53121536032923 6 25.164556401421805 7 24.08742898534802 8 20.1024748720661 9 11.929609846854063 10-11 15.574251485021184 12-13 15.662486165423845 14-15 19.808174738734643 16-17 23.1088629436912 18-19 27.074057939353985 20-21 29.14295253170082 22-23 28.728096230309554 24-25 27.241414258843587 26-27 28.70727250506008 28-29 25.66015254549814 30-31 27.527772419252706 32-33 26.53442962368675 34-35 24.5956726680728 36-37 27.57163425362275 38-39 27.69785413427598 40-41 24.74101290176695 42-43 25.58986714974302 44-45 24.29549590062186 46-47 27.584963141154624 48-49 22.975595360525116 50-51 28.56355047496405 52-53 22.15948844325833 54-55 23.306305074814237 56-57 25.39067906210623 58-59 24.681607795993923 60-61 24.20907105319078 62-63 24.063496605817953 64-65 22.597361561617575 66-67 23.098387204608645 68-69 24.093135282246646 70-71 22.592677288044072 72-73 22.442503735708176 74-75 19.74244586363739 76-77 21.990343582949755 78-79 21.101609133141107 80-81 22.148075849461073 82-83 22.99277812767882 84-85 19.95988132605823 86-87 21.495279742688595 88-89 20.330662871553386 90-91 21.464022862661782 92-93 21.45233347088064 94-95 20.51515937389148 96-97 19.411544519974807 98-99 18.808103622944934 100-101 20.48709631675569 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2348283.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.99447782622283 #Duplication Level Percentage of deduplicated Percentage of total 1 86.70104974288162 63.28697852967016 2 8.629016325640425 12.597410816881496 3 2.231856382828067 4.887395737429718 4 0.9019521451749123 2.63350103445137 5 0.45770960252143383 1.6705136716050037 6 0.25806595472005206 1.1302433765709519 7 0.17784849632943833 0.9087370685222381 8 0.12475825596911336 0.7285330999180542 9 0.08659847856741816 0.5689089651216634 >10 0.40752826733862596 5.079674054338224 >50 0.015545660793780662 0.7604131257633613 >100 0.007772366611663353 0.9351608687094278 >500 1.1932824939202322E-4 0.06441269256542827 >1k 1.1932824939180988E-4 0.1664538951891466 >5k 0.0 0.0 >10k+ 5.966412469590494E-5 4.581663063263583 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 105201 4.479911492780044 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 4.258430521363907E-5 0.0 0.0 0.0 0.0 6 4.258430521363907E-5 0.0 0.0 0.0 0.0 7 4.258430521363907E-5 0.0 0.0 4.258430521363907E-5 0.0 8 4.258430521363907E-5 0.0 0.0 4.258430521363907E-5 0.0 9 4.258430521363907E-5 0.0 0.0 4.258430521363907E-5 0.0 10-11 4.258430521363907E-5 0.0 0.0 8.516861042727814E-5 0.0 12-13 4.258430521363907E-5 0.0 0.0 8.516861042727814E-5 0.0 14-15 4.258430521363907E-5 0.0 0.0 1.0646076303409768E-4 0.0 16-17 4.258430521363907E-5 0.0 0.0 1.2775291564091722E-4 0.0 18-19 4.258430521363907E-5 0.0 0.0 1.2775291564091722E-4 0.0 20-21 4.258430521363907E-5 0.0 0.0 1.2775291564091722E-4 0.0 22-23 4.258430521363907E-5 0.0 0.0 1.2775291564091722E-4 0.0 24-25 4.258430521363907E-5 0.0 0.0 1.4904506824773675E-4 0.0 26-27 4.258430521363907E-5 0.0 0.0 2.1292152606819536E-4 0.0 28-29 4.258430521363907E-5 0.0 0.0 4.4713520474321027E-4 0.0 30-31 4.258430521363907E-5 0.0 0.0 0.001469158529870548 0.0 32-33 4.258430521363907E-5 0.0 0.0 0.004343599131791186 0.0 34-35 4.258430521363907E-5 0.0 0.0 0.008793659026616468 0.0 36-37 6.387645782045861E-5 0.0 0.0 0.015905237997294194 0.0 38-39 8.516861042727814E-5 0.0 0.0 0.02610417909596075 0.0 40-41 8.516861042727814E-5 0.0 0.0 0.03734643567236147 0.0 42-43 8.516861042727814E-5 0.0 0.0 0.04909970391132585 0.0 44-45 8.516861042727814E-5 0.0 0.0 0.0634293226157154 0.0 46-47 8.516861042727814E-5 0.0 0.0 0.08020753886988918 0.0 48-49 8.516861042727814E-5 0.0 0.0 0.09854008226436081 0.0 50-51 8.516861042727814E-5 0.0 0.0 0.11974706626075307 0.0 52-53 1.0646076303409768E-4 0.0 0.0 0.14350910856996368 0.0 54-55 1.2775291564091722E-4 0.0 0.0 0.17148699709532456 0.0 56-57 1.2775291564091722E-4 0.0 0.0 0.20282904573256288 0.0 58-59 1.2775291564091722E-4 0.0 0.0 0.2381527269072765 0.0 60-61 1.2775291564091722E-4 0.0 0.0 0.27960854803275415 0.0 62-63 1.2775291564091722E-4 0.0 0.0 0.32055335749566816 0.0 64-65 1.2775291564091722E-4 0.0 0.0 0.3639254723557595 0.0 66-67 1.4904506824773675E-4 4.258430521363907E-5 0.0 0.4142601211182809 0.0 68-69 1.7033722085455627E-4 4.258430521363907E-5 0.0 0.46895966116520027 0.0 70-71 1.7033722085455627E-4 4.258430521363907E-5 0.0 0.5315372976766428 0.0 72-73 1.7033722085455627E-4 8.516861042727814E-5 0.0 0.5962015651435538 2.1292152606819534E-5 74-75 1.7033722085455627E-4 8.516861042727814E-5 0.0 0.6610787541365329 4.258430521363907E-5 76-77 1.7033722085455627E-4 8.516861042727814E-5 0.0 0.734345051256599 4.258430521363907E-5 78-79 1.7033722085455627E-4 8.516861042727814E-5 0.0 0.822835237490541 4.258430521363907E-5 80-81 1.7033722085455627E-4 8.516861042727814E-5 0.0 0.9203745885823813 4.258430521363907E-5 82-83 1.7033722085455627E-4 8.516861042727814E-5 0.0 1.0227685504685764 4.258430521363907E-5 84-85 1.7033722085455627E-4 8.516861042727814E-5 0.0 1.1270788060893853 4.258430521363907E-5 86-87 1.7033722085455627E-4 8.516861042727814E-5 0.0 1.238032213323522 4.258430521363907E-5 88-89 1.7033722085455627E-4 8.516861042727814E-5 0.0 1.3606963044914093 4.258430521363907E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1910 0.0 55.828896 1 GTATCAA 3955 0.0 49.645676 1 ATCAACG 4780 0.0 40.90192 3 TCAACGC 4895 0.0 39.83423 4 CAACGCA 4910 0.0 39.806232 5 TATCAAC 4940 0.0 39.63 2 AACGCAG 5355 0.0 36.440006 6 ACGCAGA 6010 0.0 31.99863 7 CGCAGAG 6225 0.0 31.08942 8 GCAGAGT 6725 0.0 26.221159 9 GTACATG 4380 0.0 25.281845 1 TACATGG 4515 0.0 23.950377 2 ACATGGG 4525 0.0 23.719246 3 GTGGTAT 970 0.0 22.197634 1 GAGTACT 4575 0.0 21.837837 12-13 TAACGCC 140 3.2594663E-4 21.593416 4 GAGTACA 3280 0.0 21.256618 1 CAGAGTA 6500 0.0 21.181005 10-11 GTACTTT 4790 0.0 21.16085 14-15 GTATAGG 630 0.0 21.157381 1 >>END_MODULE