FastQCFastQC Report
Sat 18 Jun 2016
SRR1931006_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931006_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2348283
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62550.2663648291113124No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54550.23229738494040114No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32660.1390803408277452No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32100.059.382461
GTATCAA61150.041.6665151
ATCAACG77100.032.550083
CAACGCA78500.032.0264325
TCAACGC78750.031.9881064
AACGCAG82300.030.8921976
TATCAAC82350.030.7090822
ACGCAGA96400.026.2261187
CGCAGAG97550.025.771078
GTACATG67600.023.3458791
TACATGG70350.022.5008532
GCAGAGT110750.022.2707169
ACATGGG69050.022.096013
GTGGTAT17850.020.771851
CATGGGG37700.020.4238174
AGAGTAC104000.020.2955710-11
CAGAGTA107800.019.69014410-11
GAGTACT78450.019.2873412-13
TGGTATC17800.018.9608232
GTACTAG5300.018.8348541
Produced by FastQC (version 0.11.5)