##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931006_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2348283 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.365177876772094 31.0 31.0 34.0 30.0 34.0 2 31.522026518950227 31.0 31.0 34.0 30.0 34.0 3 31.588836183713802 31.0 31.0 34.0 30.0 34.0 4 35.36653418689315 37.0 35.0 37.0 33.0 37.0 5 35.24385689459064 37.0 35.0 37.0 33.0 37.0 6 35.26902294144275 37.0 35.0 37.0 32.0 37.0 7 35.15406022187275 37.0 35.0 37.0 32.0 37.0 8 35.15540886681886 37.0 35.0 37.0 32.0 37.0 9 36.65961385403718 39.0 35.0 39.0 32.0 39.0 10-11 36.59599098575427 38.5 35.0 39.0 32.0 39.0 12-13 36.487779369011314 38.0 35.0 39.0 32.0 39.0 14-15 37.55633243523033 39.0 36.0 41.0 32.0 41.0 16-17 37.509384516261456 39.0 36.0 41.0 32.0 41.0 18-19 37.5371956872319 39.0 36.0 41.0 32.0 41.0 20-21 37.520734298208524 39.0 36.0 41.0 32.0 41.0 22-23 37.41682859348724 39.0 36.0 41.0 32.0 41.0 24-25 37.293315371273394 39.0 36.0 41.0 31.5 41.0 26-27 37.04847435338927 39.0 36.0 40.0 31.0 41.0 28-29 36.865399102237674 39.0 36.0 40.0 30.5 41.0 30-31 36.62890737615526 38.5 35.0 40.0 30.0 41.0 32-33 36.46334364299362 38.0 35.0 40.0 30.0 41.0 34-35 36.54212716269717 38.0 35.0 40.0 30.0 41.0 36-37 36.60746468802951 39.0 35.0 40.0 30.0 41.0 38-39 36.59375509680903 39.0 35.0 40.0 30.0 41.0 40-41 36.475686278016745 39.0 35.0 40.0 30.0 41.0 42-43 36.33104229771284 39.0 35.0 40.0 30.0 41.0 44-45 36.15502092379836 38.5 35.0 40.0 30.0 41.0 46-47 35.90423130431894 38.0 35.0 40.0 29.0 41.0 48-49 35.681338876106494 38.0 35.0 40.0 28.0 41.0 50-51 35.41489398850139 38.0 34.5 40.0 27.5 41.0 52-53 35.204584796636524 38.0 34.0 40.0 27.0 41.0 54-55 35.0520995552921 38.0 34.0 40.0 26.0 41.0 56-57 34.88221074717144 38.0 34.0 40.0 26.0 41.0 58-59 34.67514158216876 38.0 33.5 40.0 26.0 41.0 60-61 34.37085287420639 37.5 33.0 40.0 25.5 41.0 62-63 34.11783886354413 37.0 33.0 40.0 25.0 41.0 64-65 33.80979102603902 37.0 32.5 39.5 24.5 41.0 66-67 33.48561906720783 36.0 32.0 39.0 23.5 41.0 68-69 33.14168564862072 36.0 32.0 39.0 23.0 40.0 70-71 32.768870276708554 35.5 31.0 39.0 22.0 40.0 72-73 32.34392277251081 35.0 31.0 38.0 22.0 40.0 74-75 31.832907064438146 35.0 31.0 37.0 20.0 39.5 76-77 30.45569528885573 33.5 29.5 36.0 20.0 38.5 78-79 31.187119482617724 34.0 30.0 36.0 20.0 39.0 80-81 31.157616650122662 34.0 31.0 36.0 20.0 38.5 82-83 30.886608428370856 34.0 30.0 36.0 20.0 37.0 84-85 30.551412031684425 34.0 30.0 35.0 18.5 37.0 86-87 30.21648199982711 34.0 30.0 35.0 18.0 36.5 88-89 29.87170370862456 34.0 30.0 35.0 15.0 36.0 90-91 29.5391624433682 34.0 29.0 35.0 9.0 36.0 92-93 29.221159034068723 34.0 29.0 35.0 4.5 35.5 94-95 28.847405104069654 34.0 29.0 35.0 2.0 35.0 96-97 28.431763973933293 34.0 29.0 35.0 2.0 35.0 98-99 27.946515603102352 34.0 29.0 35.0 2.0 35.0 100-101 26.81607199813651 32.5 26.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.20717931876176365 1101 2 0.26410967925567164 1101 3 0.21109940197528942 1101 4 0.27297850634754184 1101 5 0.1519137769096588 1101 6 0.2616039412297795 1101 7 0.26825875603438476 1101 8 0.2602271610911373 1101 9 0.46874577777406046 1101 10-11 0.42219708568721614 1101 12-13 0.48044065377908396 1101 14-15 0.42939000274629535 1101 16-17 0.6019320119378406 1101 18-19 0.5222325933151666 1101 20-21 0.395568543047375 1101 22-23 0.34764093785872774 1101 24-25 0.3828279306591398 1101 26-27 0.3140173071484611 1101 28-29 0.32142868173106365 1101 30-31 0.34855483501757334 1101 32-33 0.34334612798148356 1101 34-35 0.3586829618279026 1101 36-37 0.4855450572622644 1101 38-39 0.574377074789858 1101 40-41 0.4947316579650689 1101 42-43 0.504516013148141 1101 44-45 0.5406721141176405 1101 46-47 0.49881311172600107 1101 48-49 0.5546751531691925 1101 50-51 0.7209139820538084 1101 52-53 0.5366600608807914 1101 54-55 0.5248745077817318 1101 56-57 0.5760225097509419 1101 58-59 0.5663479022334101 1101 60-61 0.6874557466258793 1101 62-63 0.3324903619100965 1101 64-65 0.4379976968192665 1101 66-67 0.38262871141705546 1101 68-69 0.25461606346954824 1101 70-71 0.2964528842987306 1101 72-73 0.44983178447369454 1101 74-75 0.4058541075782607 1101 76-77 0.41303460328132857 1101 78-79 0.4863354079974229 1101 80-81 0.4173242612991217 1101 82-83 0.4361025455559364 1101 84-85 0.4980841578198394 1101 86-87 0.5387349824562051 1101 88-89 0.4525510889460449 1101 90-91 0.4140938026808705 1101 92-93 0.23069065194172467 1101 94-95 0.40270316746314805 1101 96-97 0.5785664349904494 1101 98-99 0.5307504671737213 1101 100-101 0.6209814689129161 1102 1 0.3062881062341454 1102 2 0.4174640384897259 1102 3 0.3901940268758146 1102 4 0.27768081409549694 1102 5 0.2959567320735772 1102 6 0.3538749054532815 1102 7 0.42642393781057564 1102 8 0.45330280424997227 1102 9 0.48198249584149977 1102 10-11 0.4975007879894022 1102 12-13 0.48297475246633326 1102 14-15 0.5876289189339161 1102 16-17 0.6453497549414209 1102 18-19 0.6241297240727164 1102 20-21 0.5898941931622872 1102 22-23 0.5210820115430508 1102 24-25 0.6176556842915986 1102 26-27 0.6826511058896898 1102 28-29 0.6148690319834316 1102 30-31 0.565716588839237 1102 32-33 0.6128648787814299 1102 34-35 0.7012161701373572 1102 36-37 0.8158630473456796 1102 38-39 0.6554136275809128 1102 40-41 0.877364908567472 1102 42-43 0.7392853357375841 1102 44-45 0.766371408493228 1102 46-47 0.7052475520957344 1102 48-49 0.581320056591025 1102 50-51 0.4948950559607326 1102 52-53 0.4860427786477217 1102 54-55 0.71423699727805 1102 56-57 0.44093724216459407 1102 58-59 0.3971938585269825 1102 60-61 0.40356678508463517 1102 62-63 0.24858025389613658 1102 64-65 0.35532229239298374 1102 66-67 0.21197285078343953 1102 68-69 0.32636312383159094 1102 70-71 0.42907883102075317 1102 72-73 0.4410034799199707 1102 74-75 0.5139306689392065 1102 76-77 0.4083667281122949 1102 78-79 0.516124805823786 1102 80-81 0.5289372919782025 1102 82-83 0.3860702693607436 1102 84-85 0.4523983860570553 1102 86-87 0.3862230040923862 1102 88-89 0.38661130909195407 1102 90-91 0.2736540951699773 1102 92-93 0.22401664429560597 1102 94-95 0.3376847199481361 1102 96-97 0.35667594032993577 1102 98-99 0.5981263290316079 1102 100-101 0.7570113716947944 1103 1 0.3651747759899564 1103 2 0.34500544589312554 1103 3 0.3827432951664953 1103 4 0.3095844834906032 1103 5 0.3899465242135065 1103 6 0.32615064772021896 1103 7 0.38035030898271316 1103 8 0.3730408180224387 1103 9 0.4614107215259651 1103 10-11 0.42347138683364705 1103 12-13 0.43618886975747984 1103 14-15 0.5609493002263051 1103 16-17 0.5014991966712685 1103 18-19 0.5507176951698369 1103 20-21 0.5482901820222068 1103 22-23 0.5027602750432791 1103 24-25 0.5583047875618306 1103 26-27 0.545202362502323 1103 28-29 0.6187443775442034 1103 30-31 0.38817218697507627 1103 32-33 0.5748866462867142 1103 34-35 0.5566311134205577 1103 36-37 0.589833192400782 1103 38-39 0.6412275836160717 1103 40-41 0.6542395094486224 1103 42-43 0.7816664522231349 1103 44-45 0.8363379252885608 1103 46-47 0.7942665393725292 1103 48-49 0.629151348329529 1103 50-51 0.724457433487288 1103 52-53 0.7471813171181623 1103 54-55 0.69229085102927 1103 56-57 0.6508698171749785 1103 58-59 0.6526907473678136 1103 60-61 0.5504650199612371 1103 62-63 0.5715011912342192 1103 64-65 0.5299586450781746 1103 66-67 0.516181060602392 1103 68-69 0.3956320168256511 1103 70-71 0.550394275388463 1103 72-73 0.6205534860457789 1103 74-75 0.5051042819563101 1103 76-77 0.6665434026660861 1103 78-79 0.6668658749729026 1103 80-81 0.5073212208613 1103 82-83 0.6062888170849199 1103 84-85 0.3867851307599395 1103 86-87 0.4432697060446351 1103 88-89 0.4653287524590013 1103 90-91 0.47471476739390894 1103 92-93 0.4937947153974527 1103 94-95 0.42774877403049416 1103 96-97 0.6103412458573345 1103 98-99 0.45795433747222347 1103 100-101 0.8029237274216712 1104 1 0.3051375308962996 1104 2 0.38879365465685467 1104 3 0.27508648262073265 1104 4 0.2573275561893311 1104 5 0.3230547131224526 1104 6 0.34566917408611886 1104 7 0.3471611891548392 1104 8 0.3650259610045339 1104 9 0.5043978911717559 1104 10-11 0.5165832112659814 1104 12-13 0.4189957220740226 1104 14-15 0.5344229634395532 1104 16-17 0.5683991124323171 1104 18-19 0.5800253396765171 1104 20-21 0.5036000392692017 1104 22-23 0.46298983237777946 1104 24-25 0.43404299481213116 1104 26-27 0.5469983739130342 1104 28-29 0.5639366294135257 1104 30-31 0.5783129551687765 1104 32-33 0.5334075241647227 1104 34-35 0.6154090747387855 1104 36-37 0.6223572330849052 1104 38-39 0.7031007018646918 1104 40-41 0.5111095487496655 1104 42-43 0.4781327499453738 1104 44-45 0.6171935204136503 1104 46-47 0.5567141133222648 1104 48-49 0.51251133610306 1104 50-51 0.40057480697214487 1104 52-53 0.4233717980343883 1104 54-55 0.3503203378035238 1104 56-57 0.5977262555165552 1104 58-59 0.52334426660574 1104 60-61 0.34760314520551816 1104 62-63 0.2985843684699532 1104 64-65 0.3208750301902583 1104 66-67 0.4458780931519044 1104 68-69 0.29028801245376457 1104 70-71 0.4129457710556821 1104 72-73 0.24174278186077913 1104 74-75 0.36132338393572283 1104 76-77 0.31978137545986485 1104 78-79 0.5376288899564514 1104 80-81 0.5522654202083928 1104 82-83 0.4689107737606584 1104 84-85 0.5247824370322789 1104 86-87 0.4609744403462628 1104 88-89 0.5093019627204356 1104 90-91 0.4880744888711348 1104 92-93 0.2946495761401984 1104 94-95 0.6042708848107452 1104 96-97 0.7488904139956603 1104 98-99 0.7298792478701337 1104 100-101 0.9195510037381567 1105 1 0.2939332265102621 1105 2 0.3184905989610982 1105 3 0.37459065715545137 1105 4 0.2633381326733115 1105 5 0.28194400691706534 1105 6 0.30713535720440177 1105 7 0.2527513565714443 1105 8 0.3054924773580723 1105 9 0.3805985611499878 1105 10-11 0.43843727241627306 1105 12-13 0.42658112740954834 1105 14-15 0.4545695187419838 1105 16-17 0.4523495318345354 1105 18-19 0.5106109925025493 1105 20-21 0.4872939227459909 1105 22-23 0.38425902669550993 1105 24-25 0.3984504824067514 1105 26-27 0.4347239166869912 1105 28-29 0.4738980735108811 1105 30-31 0.3749675948362423 1105 32-33 0.473782936730089 1105 34-35 0.48535857953041983 1105 36-37 0.6049275843720139 1105 38-39 0.6879405280886814 1105 40-41 0.6090096632708608 1105 42-43 0.5787075157875279 1105 44-45 0.6957883199950601 1105 46-47 0.656478201605573 1105 48-49 0.662260142984465 1105 50-51 0.6458144310464107 1105 52-53 0.6593225708088681 1105 54-55 0.7218017990758128 1105 56-57 0.7926158526656693 1105 58-59 0.6792217925841229 1105 60-61 0.6998614635822449 1105 62-63 0.6200350720640202 1105 64-65 0.5655172017927725 1105 66-67 0.8075896069168635 1105 68-69 0.5225318679011224 1105 70-71 0.5837426385144511 1105 72-73 0.5620843252531316 1105 74-75 0.6823938388310573 1105 76-77 0.5452837941761253 1105 78-79 0.6595163395895369 1105 80-81 0.60259246819232 1105 82-83 0.673201437392482 1105 84-85 0.7891584036905641 1105 86-87 0.8353942829786796 1105 88-89 0.7739829890330299 1105 90-91 0.7849260328935905 1105 92-93 0.6269708616877274 1105 94-95 0.7140398254669407 1105 96-97 0.6829795027823877 1105 98-99 0.8083356866181823 1105 100-101 1.0982073186759962 1106 1 0.2497810395062352 1106 2 0.2995235371412619 1106 3 0.23328365044234545 1106 4 0.17262501749069514 1106 5 0.216769227960377 1106 6 0.1532657196708982 1106 7 0.20148654155463674 1106 8 0.24950925334965746 1106 9 0.31616683237476195 1106 10-11 0.23613173534509002 1106 12-13 0.33760236073408834 1106 14-15 0.34119934324338175 1106 16-17 0.31372000681221834 1106 18-19 0.3154852887428632 1106 20-21 0.26441372147539965 1106 22-23 0.29243085216891274 1106 24-25 0.2700591561985206 1106 26-27 0.2661486625654419 1106 28-29 0.4552113699299767 1106 30-31 0.37837941341646797 1106 32-33 0.5001233798945748 1106 34-35 0.4553313650149846 1106 36-37 0.49055150771570055 1106 38-39 0.4067321784313762 1106 40-41 0.35584455994089836 1106 42-43 0.3523248131566916 1106 44-45 0.2375359611132808 1106 46-47 0.24366235283734028 1106 48-49 0.36605258720486944 1106 50-51 0.25927480525326274 1106 52-53 0.26225874045860564 1106 54-55 0.4001656300069669 1106 56-57 0.40162534821769214 1106 58-59 0.36040364737936414 1106 60-61 0.3029815805081668 1106 62-63 0.41927259446847387 1106 64-65 0.33214353452305545 1106 66-67 0.2804016347451821 1106 68-69 0.3603649173532446 1106 70-71 0.37608859235207603 1106 72-73 0.3733975022678564 1106 74-75 0.44088147273337697 1106 76-77 0.47673834274046456 1106 78-79 0.3461243969965757 1106 80-81 0.34089156581798363 1106 82-83 0.46668875180743186 1106 84-85 0.5977487576244869 1106 86-87 0.5867138626494715 1106 88-89 0.6077437039618658 1106 90-91 0.5356148670357541 1106 92-93 0.6191605248834939 1106 94-95 0.6775972076583905 1106 96-97 0.6555524798302841 1106 98-99 0.6984939909986458 1106 100-101 0.8294900378943524 1107 1 0.09167041066983828 1107 2 0.11486621873170932 1107 3 0.0703038144335828 1107 4 -0.02642146220976116 1107 5 0.13095785849404962 1107 6 0.1832753489353891 1107 7 0.18165176554378348 1107 8 0.21395432986450658 1107 9 0.11738793033563155 1107 10-11 0.1965013488598899 1107 12-13 0.22844592378987016 1107 14-15 0.2583513121061358 1107 16-17 0.21499604888435186 1107 18-19 0.20549521977460472 1107 20-21 0.21130477242317625 1107 22-23 0.26281360058475656 1107 24-25 0.13073586604304666 1107 26-27 0.2012035352477568 1107 28-29 0.15460359785028288 1107 30-31 0.16434481259113198 1107 32-33 0.26543794737141724 1107 34-35 0.3236164618702375 1107 36-37 0.2975576282938661 1107 38-39 0.1854073303222279 1107 40-41 0.1541826570768805 1107 42-43 0.22680705359231723 1107 44-45 0.2329135449752826 1107 46-47 0.1690800958205685 1107 48-49 0.16003111068661724 1107 50-51 0.23119584432136975 1107 52-53 0.23666197566033276 1107 54-55 0.3021967322583876 1107 56-57 0.25243996729994933 1107 58-59 0.22448727735686447 1107 60-61 0.24961259797583324 1107 62-63 -0.01893357901526116 1107 64-65 -0.09343769247220735 1107 66-67 0.11448558735681047 1107 68-69 0.08244362178297848 1107 70-71 0.1606982058540396 1107 72-73 0.22582432892681226 1107 74-75 0.26475517864546916 1107 76-77 0.21149809347429382 1107 78-79 0.2849503169412557 1107 80-81 0.27710126002663316 1107 82-83 0.31656235466921245 1107 84-85 0.27242881612239245 1107 86-87 0.35137850694052375 1107 88-89 0.48692348131863383 1107 90-91 0.5933373236715589 1107 92-93 0.38686788192085686 1107 94-95 0.4552670572739679 1107 96-97 0.4988590334605085 1107 98-99 0.576476410488187 1107 100-101 0.7303473779091547 1108 1 0.10402570024279001 1108 2 0.12202393094980124 1108 3 0.0517309136459545 1108 4 -0.06748009037101355 1108 5 0.07708537858365361 1108 6 0.052635127207466326 1108 7 0.08010758896789838 1108 8 0.09399679459899346 1108 9 0.18282543246947114 1108 10-11 0.19494306267858263 1108 12-13 0.1316646289843959 1108 14-15 0.11226616937501888 1108 16-17 0.1077629667785871 1108 18-19 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-1.1669199379140522 2316 90-91 -1.079152747273458 2316 92-93 -1.184386259651241 2316 94-95 -1.372989315138657 2316 96-97 -1.4333877016191678 2316 98-99 -1.5686206536781384 2316 100-101 -2.006722671671916 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 13.0 4 25.0 5 71.0 6 119.0 7 296.0 8 764.0 9 1418.0 10 2575.0 11 4239.0 12 6163.0 13 7994.0 14 10035.0 15 12599.0 16 15262.0 17 18068.0 18 15389.0 19 11605.0 20 11243.0 21 11701.0 22 12933.0 23 15232.0 24 18705.0 25 23532.0 26 30722.0 27 39219.0 28 50214.0 29 63395.0 30 78304.0 31 95373.0 32 115375.0 33 141228.0 34 176143.0 35 220401.0 36 290270.0 37 397770.0 38 378585.0 39 71279.0 40 21.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.217283450097675 21.769738822925188 14.996910369192781 23.016067357784358 2 14.823296129903527 22.033013341532936 40.744591008572534 22.399099519991005 3 17.987610258436877 26.96265660179377 31.31171395683478 23.738019182934572 4 12.372442341506863 18.48488674765403 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20.751294945141424 28.041931374454894 30.606568976123015 20.60020470428067 26-27 20.65992239638994 28.294187650153912 30.41307324647163 20.632816706984517 28-29 20.66639015210714 28.25190277645884 30.46141450824199 20.620292563192024 30-31 20.94273134694797 28.04854236416185 30.351136189174856 20.65759009971533 32-33 20.85084189088375 28.237094566549132 30.207998575676047 20.70406496689107 34-35 21.007273112906805 28.199422222581873 30.371390009127058 20.421914655384263 36-37 20.983970481992742 28.006484797370312 30.36906238934633 20.640482331290617 38-39 20.78545164662043 28.19651578953868 30.40756008814113 20.610472475699762 40-41 20.91323714782135 28.108838960242565 30.411180288215856 20.566743603720234 42-43 20.907930744216245 28.21862074883929 29.99967419364524 20.873774313299222 44-45 20.786389570169383 28.431598846902894 30.032502722401112 20.74950886052661 46-47 21.00916870506761 28.390597014575764 29.682971957693248 20.917262322663376 48-49 21.046448363793168 28.512917702549466 29.473783957879718 20.96684997577764 50-51 21.12906993488104 28.629856588961246 29.174957200684787 21.066116275472925 52-53 21.129069974908088 28.6653835421697 29.206977474407676 20.998569008514536 54-55 21.07886261604443 28.672368451035123 29.2867360361759 20.96203289674455 56-57 21.082278929794516 28.766291964004527 29.10939499265053 21.04203411355043 58-59 21.070147006522973 28.628599295953116 29.347787661503798 20.953466036020114 60-61 21.05376258353403 28.86508839838721 29.278966511346898 20.80218250673186 62-63 21.04306308940556 28.732033034991094 29.32266316069182 20.902240714911528 64-65 20.82011668994987 28.61433085773623 29.287235075713998 21.278317376599897 66-67 20.94612580500487 28.74397573837163 29.112822677031698 21.197075779591806 68-69 20.978566156239474 28.68848714810386 29.201290803473505 21.13165589218316 70-71 20.991245990733763 28.496033206158028 29.257697748493406 21.255023054614806 72-73 21.009294251128193 28.742290335514358 29.067738413110057 21.180677000247393 74-75 20.97801911320192 28.923306136837102 28.91826724448812 21.180407505472857 76-77 20.942347380334198 28.799360382643783 29.056393698394896 21.201898538627116 78-79 20.827447491619996 28.781265584083464 29.047006812600053 21.34428011169649 80-81 20.938340906952018 28.949300403741795 28.857957069058543 21.254401620247645 82-83 21.027171767627667 28.85991594709837 29.014582143634303 21.098330141639657 84-85 21.052083586177645 28.931989883672454 28.739955959311548 21.275970570838354 86-87 20.918773418706348 29.06491679239683 28.80830376917944 21.208006019717384 88-89 20.946398260515558 28.838529614415986 28.773396891855405 21.441675233213047 90-91 21.0037576445933 28.776845908038535 28.986138641690374 21.233257805677795 92-93 21.03833294290521 28.836637774985626 28.85456907383351 21.270460208275658 94-95 20.746989747575295 28.75925433039849 28.91028329908761 21.5834726229386 96-97 20.867186976013322 28.651152491235614 28.92445862028951 21.557201912461554 98-99 20.703083912799265 28.759395694641576 28.84230733689253 21.695213055666628 100-101 20.72094802883639 28.674716803724255 28.854571616794054 21.7497635506453 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 184.0 1 131.0 2 136.0 3 196.0 4 168.0 5 160.5 6 300.5 7 655.5 8 1122.5 9 1402.5 10 1337.5 11 1198.5 12 1244.0 13 1440.0 14 1740.5 15 2016.5 16 2281.5 17 2603.5 18 2974.5 19 3361.5 20 3900.5 21 4627.5 22 5523.5 23 6831.0 24 8723.5 25 11183.5 26 14485.5 27 18698.0 28 23402.0 29 29594.0 30 37261.0 31 44855.5 32 53728.0 33 61683.0 34 67572.0 35 73340.0 36 80776.0 37 88694.5 38 92697.0 39 93773.5 40 93464.0 41 92823.5 42 92485.5 43 93548.0 44 94490.0 45 96217.0 46 96392.0 47 93937.5 48 92608.5 49 89809.0 50 84369.0 51 77206.0 52 70957.0 53 65328.0 54 58656.5 55 50078.0 56 40893.0 57 32646.0 58 25471.5 59 19626.5 60 14267.5 61 9923.0 62 7021.0 63 4499.0 64 2444.5 65 1396.0 66 757.0 67 415.5 68 242.5 69 136.5 70 80.0 71 53.0 72 37.0 73 27.5 74 23.0 75 15.0 76 8.0 77 5.0 78 4.5 79 2.5 80 1.5 81 2.0 82 1.0 83 0.5 84 0.5 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.004513936352645741 2 0.008602029653155091 3 0.0021292152606819533 4 0.1724238518100246 5 0.002171799565895593 6 3.406744417091126E-4 7 0.0 8 8.091017990591424E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 3.832587469227516E-4 24-25 7.452253412386838E-4 26-27 0.0028744406019206373 28-29 1.703372208545563E-4 30-31 0.0063237693242254025 32-33 0.021398613369853634 34-35 0.013626977668364503 36-37 9.155625620932401E-4 38-39 1.4904506824773675E-4 40-41 0.0018311251241864802 42-43 0.011178380118580257 44-45 0.007324500496745921 46-47 0.010284109709093835 48-49 0.010454446929948392 50-51 0.19143774408791445 52-53 0.11525442206071414 54-55 0.017949284647548868 56-57 0.006579275155507236 58-59 0.01822608263143752 60-61 0.12377128310344196 62-63 0.2391321659271902 64-65 0.24488104713103145 66-67 0.2548031902458094 68-69 0.24979953438320682 70-71 0.2378333446181742 72-73 0.16279979883174217 74-75 0.2767341074308335 76-77 0.21321961620469082 78-79 0.0057062968986276354 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 4.2584305213639075E-5 90-91 0.005812757661661733 92-93 0.01843900415750572 94-95 0.002469889702391066 96-97 2.1292152606819538E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2348283.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.67209799134912 #Duplication Level Percentage of deduplicated Percentage of total 1 73.41905828000334 40.139739487169145 2 15.148805963989412 16.564340082303264 3 5.051705579509633 8.285620273991874 4 2.228528410637121 4.873532945714328 5 1.1747526652351339 3.21130964146669 6 0.6737613577524141 2.2101568184294647 7 0.4662715207760396 1.784442959211011 8 0.30856131690681743 1.3495755643415404 9 0.2263385676794352 1.113696391625252 >10 1.172518883447032 11.591551647980289 >50 0.08530466432285809 3.2279614498225815 >100 0.042125379613744886 4.188459948814138 >500 0.0016419176783125477 0.5689950751515981 >1k 4.6911933666025674E-4 0.39005935397446184 >5k 1.563731122200856E-4 0.5005583600044874 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6255 0.2663648291113124 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5455 0.23229738494040114 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3266 0.1390803408277452 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 4.258430521363907E-5 14-15 0.0 0.0 0.0 0.0 4.258430521363907E-5 16-17 0.0 0.0 0.0 0.0 4.258430521363907E-5 18-19 0.0 0.0 0.0 4.258430521363907E-5 4.258430521363907E-5 20-21 0.0 4.258430521363907E-5 0.0 8.516861042727814E-5 8.516861042727814E-5 22-23 0.0 4.258430521363907E-5 0.0 8.516861042727814E-5 8.516861042727814E-5 24-25 0.0 4.258430521363907E-5 0.0 1.4904506824773675E-4 8.516861042727814E-5 26-27 0.0 4.258430521363907E-5 0.0 2.980901364954735E-4 8.516861042727814E-5 28-29 0.0 4.258430521363907E-5 0.0 8.516861042727814E-4 8.516861042727814E-5 30-31 0.0 4.258430521363907E-5 0.0 0.003023485670168374 8.516861042727814E-5 32-33 0.0 4.258430521363907E-5 0.0 0.007963265074950506 8.516861042727814E-5 34-35 0.0 4.258430521363907E-5 0.0 0.015415518487337344 8.516861042727814E-5 36-37 0.0 4.258430521363907E-5 0.0 0.028723113866599554 8.516861042727814E-5 38-39 0.0 4.258430521363907E-5 0.0 0.0474176238553871 8.516861042727814E-5 40-41 0.0 4.258430521363907E-5 0.0 0.07077511526506813 8.516861042727814E-5 42-43 0.0 4.258430521363907E-5 0.0 0.09398356160650143 8.516861042727814E-5 44-45 0.0 4.258430521363907E-5 0.0 0.12202532658968276 1.0646076303409768E-4 46-47 0.0 4.258430521363907E-5 0.0 0.15611406291320085 1.2775291564091722E-4 48-49 0.0 4.258430521363907E-5 0.0 0.19220426158175996 1.7033722085455627E-4 50-51 0.0 4.258430521363907E-5 0.0 0.2333194082655285 1.7033722085455627E-4 52-53 0.0 4.258430521363907E-5 0.0 0.2743280941862629 1.7033722085455627E-4 54-55 0.0 4.258430521363907E-5 0.0 0.32157538082079545 1.7033722085455627E-4 56-57 0.0 4.258430521363907E-5 0.0 0.3750612681691261 1.7033722085455627E-4 58-59 0.0 4.258430521363907E-5 0.0 0.4347644640786481 1.7033722085455627E-4 60-61 0.0 4.258430521363907E-5 0.0 0.5061144674641004 1.7033722085455627E-4 62-63 0.0 4.258430521363907E-5 0.0 0.5769747513395957 1.7033722085455627E-4 64-65 0.0 4.258430521363907E-5 0.0 0.6504752621383368 1.7033722085455627E-4 66-67 0.0 4.258430521363907E-5 0.0 0.7351328609030513 1.7033722085455627E-4 68-69 0.0 4.258430521363907E-5 0.0 0.8314159749910892 1.7033722085455627E-4 70-71 0.0 4.258430521363907E-5 0.0 0.9384729182981779 1.7033722085455627E-4 72-73 0.0 4.258430521363907E-5 0.0 1.051044529130433 1.7033722085455627E-4 74-75 0.0 4.258430521363907E-5 0.0 1.164020690862217 1.9162937346137583E-4 76-77 0.0 4.258430521363907E-5 0.0 1.2949035529363369 2.1292152606819536E-4 78-79 0.0 4.258430521363907E-5 0.0 1.4509111550865037 2.1292152606819536E-4 80-81 0.0 4.258430521363907E-5 0.0 1.6204605662946077 2.1292152606819536E-4 82-83 0.0 4.258430521363907E-5 0.0 1.8027852690668031 2.1292152606819536E-4 84-85 0.0 4.258430521363907E-5 0.0 1.988538008408697 2.1292152606819536E-4 86-87 0.0 4.258430521363907E-5 0.0 2.1873002529933574 2.1292152606819536E-4 88-89 2.1292152606819534E-5 4.258430521363907E-5 0.0 2.4094625732929122 2.1292152606819536E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3210 0.0 59.38246 1 GTATCAA 6115 0.0 41.666515 1 ATCAACG 7710 0.0 32.55008 3 CAACGCA 7850 0.0 32.026432 5 TCAACGC 7875 0.0 31.988106 4 AACGCAG 8230 0.0 30.892197 6 TATCAAC 8235 0.0 30.709082 2 ACGCAGA 9640 0.0 26.226118 7 CGCAGAG 9755 0.0 25.77107 8 GTACATG 6760 0.0 23.345879 1 TACATGG 7035 0.0 22.500853 2 GCAGAGT 11075 0.0 22.270716 9 ACATGGG 6905 0.0 22.09601 3 GTGGTAT 1785 0.0 20.77185 1 CATGGGG 3770 0.0 20.423817 4 AGAGTAC 10400 0.0 20.29557 10-11 CAGAGTA 10780 0.0 19.690144 10-11 GAGTACT 7845 0.0 19.28734 12-13 TGGTATC 1780 0.0 18.960823 2 GTACTAG 530 0.0 18.834854 1 >>END_MODULE