FastQCFastQC Report
Sat 18 Jun 2016
SRR1931005_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931005_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2587763
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1152444.453421739162358No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21700.055.7206461
GTATCAA43050.047.9618341
ATCAACG48750.041.53913
TCAACGC50850.040.3142624
CAACGCA51650.039.5831345
TATCAAC51700.039.1375432
AACGCAG55150.036.7568976
ACGCAGA61750.032.257417
CGCAGAG62300.031.1322368
GCAGAGT69800.026.195889
GAGTACT45850.023.04243712-13
CAGAGTA65850.022.12064410-11
GTGTAGC9500.021.5724891
AGAGTAC61900.021.423210-11
GTACATG40500.020.7468741
GTGGTAT12500.020.4938661
GTACTTT52800.019.8408414-15
TACATGG40800.019.7118072
ACATGGG41100.019.1200263
AGTACTT52500.019.0260712-13
Produced by FastQC (version 0.11.5)