##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931005_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2587763 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.86657201606175 30.0 2.0 31.0 2.0 33.0 2 20.478238926825988 28.0 2.0 31.0 2.0 33.0 3 21.205147843909973 28.0 2.0 31.0 2.0 34.0 4 22.72390400511948 32.0 2.0 35.0 2.0 37.0 5 22.062229037203174 32.0 2.0 35.0 2.0 37.0 6 23.113521215041718 32.0 2.0 35.0 2.0 37.0 7 23.34635629306084 32.0 2.0 35.0 2.0 37.0 8 23.845970824994406 32.0 2.0 35.0 2.0 37.0 9 25.263707302407525 33.0 10.0 37.0 2.0 39.0 10-11 25.704279719587923 33.5 9.0 37.0 2.0 39.0 12-13 25.658945969936198 34.0 5.0 37.0 2.0 39.0 14-15 25.676645233740494 34.0 2.0 38.0 2.0 40.0 16-17 24.48877447432396 32.5 2.0 38.0 2.0 40.0 18-19 23.386777498557635 31.5 2.0 38.0 2.0 40.0 20-21 23.046227958279022 31.5 2.0 38.0 2.0 40.0 22-23 22.68415963903959 30.0 2.0 38.0 2.0 40.0 24-25 22.533610303571077 30.0 2.0 38.0 2.0 40.0 26-27 22.260112691927354 30.0 2.0 38.0 2.0 40.0 28-29 22.10066377794257 29.5 2.0 38.0 2.0 40.0 30-31 22.13602945864826 30.0 2.0 37.0 2.0 40.0 32-33 22.039283543353854 30.0 2.0 37.5 2.0 40.0 34-35 21.732063755452103 29.0 2.0 37.0 2.0 40.0 36-37 21.265556196606873 27.0 2.0 37.0 2.0 40.0 38-39 21.187288016715595 27.0 2.0 37.0 2.0 39.0 40-41 21.09679537886584 27.0 2.0 37.0 2.0 39.0 42-43 20.971913192977873 27.0 2.0 37.0 2.0 39.0 44-45 20.603685113358527 26.0 2.0 36.5 2.0 39.0 46-47 20.271847731032558 25.0 2.0 36.0 2.0 39.0 48-49 20.075511745086395 24.0 2.0 36.0 2.0 39.0 50-51 17.598097468740377 12.0 2.0 33.5 2.0 38.5 52-53 19.634729880595714 24.0 2.0 35.5 2.0 38.5 54-55 20.509987390653627 26.5 2.0 37.0 2.0 39.0 56-57 20.51781963804259 26.5 2.0 37.0 2.0 40.0 58-59 20.264220293744057 26.0 2.0 37.0 2.0 39.5 60-61 19.988304570395357 26.0 2.0 36.0 2.0 39.0 62-63 19.561121903358227 24.0 2.0 36.0 2.0 39.0 64-65 19.230495026012818 23.0 2.0 35.5 2.0 39.0 66-67 18.895890775159856 22.0 2.0 35.0 2.0 39.0 68-69 18.46892431803067 21.0 2.0 34.5 2.0 38.0 70-71 18.13854359923996 20.0 2.0 34.0 2.0 38.0 72-73 17.742931829537714 18.0 2.0 34.0 2.0 37.5 74-75 17.299773781447527 13.5 2.0 33.5 2.0 37.0 76-77 16.894041687743428 10.0 2.0 33.0 2.0 36.0 78-79 16.53201123905087 7.5 2.0 32.5 2.0 36.0 80-81 16.10584218879395 7.0 2.0 32.0 2.0 35.0 82-83 15.649088807591731 2.0 2.0 31.5 2.0 35.0 84-85 15.26086005557696 2.0 2.0 31.0 2.0 35.0 86-87 14.778780166499018 2.0 2.0 31.0 2.0 34.0 88-89 14.359540460235346 2.0 2.0 31.0 2.0 34.0 90-91 13.879558908601755 2.0 2.0 30.5 2.0 34.0 92-93 13.323266272838742 2.0 2.0 30.0 2.0 34.0 94-95 12.745277484839223 2.0 2.0 29.0 2.0 34.0 96-97 12.0854840648081 2.0 2.0 29.0 2.0 34.0 98-99 11.379786325100095 2.0 2.0 29.0 2.0 34.0 100-101 10.323938668262898 2.0 2.0 25.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.875404729873189 1101 2 9.644691579109889 1101 3 9.027891332347611 1101 4 11.0939729123026 1101 5 11.860883387904398 1101 6 11.026537024608125 1101 7 10.543568324716745 1101 8 9.783824627435482 1101 9 9.779681141345012 1101 10-11 9.344470790691268 1101 12-13 9.205556184925484 1101 14-15 9.970126489138039 1101 16-17 11.078158502545577 1101 18-19 11.945166673939582 1101 20-21 11.905010579511046 1101 22-23 11.991451200325965 1101 24-25 12.10153928576457 1101 26-27 11.98124801775418 1101 28-29 12.040306532852444 1101 30-31 11.620419744075278 1101 32-33 11.543391807809204 1101 34-35 11.388470563660082 1101 36-37 11.944400764069709 1101 38-39 11.573027110665063 1101 40-41 11.361233836339121 1101 42-43 11.069836892559753 1101 44-45 11.280342697551443 1101 46-47 10.95800772781067 1101 48-49 10.977484354489924 1101 50-51 12.889530867271667 1101 52-53 12.005104906592358 1101 54-55 11.743202383040643 1101 56-57 11.63154067775746 1101 58-59 11.51567382601986 1101 60-61 11.204748978724371 1101 62-63 11.175227819419135 1101 64-65 10.896740570885605 1101 66-67 10.696714246198962 1101 68-69 10.772089015488309 1101 70-71 10.658153303571549 1101 72-73 10.503134582115202 1101 74-75 10.193158496764209 1101 76-77 10.110768224934592 1101 78-79 9.667225012955289 1101 80-81 9.696370136163726 1101 82-83 9.283454990472274 1101 84-85 9.01283269898444 1101 86-87 8.953152749897185 1101 88-89 8.700115045075608 1101 90-91 8.461145908544626 1101 92-93 8.306823152829475 1101 94-95 8.21096995549682 1101 96-97 8.202733657506505 1101 98-99 7.673478900795793 1101 100-101 6.874066615934812 1102 1 7.874689338043485 1102 2 9.347773434766502 1102 3 8.719518194438386 1102 4 10.795676330043445 1102 5 11.594622400750918 1102 6 10.5860126947427 1102 7 10.253978584482496 1102 8 9.615009604207057 1102 9 9.522423622097048 1102 10-11 8.65128446165464 1102 12-13 8.789274477581813 1102 14-15 9.481287037895918 1102 16-17 10.310292245920564 1102 18-19 11.59130944695535 1102 20-21 11.595036534275597 1102 22-23 11.814788677697333 1102 24-25 11.607146823551656 1102 26-27 11.745504599140904 1102 28-29 11.575565753337077 1102 30-31 11.539837833891852 1102 32-33 11.34669395180362 1102 34-35 11.14905421870164 1102 36-37 11.21785929097324 1102 38-39 11.345754978230328 1102 40-41 11.094117868706238 1102 42-43 10.693108937751575 1102 44-45 10.619095996423827 1102 46-47 10.632942840899286 1102 48-49 10.36864446703327 1102 50-51 12.263573921645808 1102 52-53 11.19448962599736 1102 54-55 10.728864011429483 1102 56-57 10.327780508506219 1102 58-59 10.781712343762884 1102 60-61 10.801361772896545 1102 62-63 10.824308497296517 1102 64-65 10.3975911892197 1102 66-67 10.587481765665036 1102 68-69 10.372946177040358 1102 70-71 10.094001413672096 1102 72-73 9.965155564034106 1102 74-75 9.552697418005678 1102 76-77 9.449229260071672 1102 78-79 8.983469642063884 1102 80-81 8.57781575111716 1102 82-83 8.638852054748485 1102 84-85 8.521179664021844 1102 86-87 8.355421065454777 1102 88-89 8.131206235723168 1102 90-91 7.8054107780074276 1102 92-93 7.342839205524195 1102 94-95 7.346911851721378 1102 96-97 7.139024525355312 1102 98-99 6.648346400632214 1102 100-101 5.874049167353601 1103 1 8.024811411645874 1103 2 9.516125780111341 1103 3 8.982261012936661 1103 4 10.99297146023994 1103 5 11.790703072277093 1103 6 10.986620007775905 1103 7 10.390393847323658 1103 8 9.807649448997399 1103 9 9.569318382682969 1103 10-11 9.00607337099376 1103 12-13 8.941654933752915 1103 14-15 9.602810534867302 1103 16-17 10.632243481764093 1103 18-19 11.452022922592107 1103 20-21 12.061288644512619 1103 22-23 12.164097666616552 1103 24-25 12.075266446475208 1103 26-27 11.995323602113022 1103 28-29 11.640370640633474 1103 30-31 11.542483529175719 1103 32-33 11.647742855735832 1103 34-35 11.551628435852873 1103 36-37 11.723757701631012 1103 38-39 11.428036227105633 1103 40-41 11.028085758787267 1103 42-43 10.912680094065212 1103 44-45 10.988835051020903 1103 46-47 10.65656473162144 1103 48-49 10.834838616372352 1103 50-51 12.632490433680331 1103 52-53 11.461719287581044 1103 54-55 10.908167409319123 1103 56-57 10.757405097653429 1103 58-59 10.715184950647384 1103 60-61 10.685391641381806 1103 62-63 10.647105920401028 1103 64-65 10.534600498953534 1103 66-67 10.661854176779801 1103 68-69 10.43644537479457 1103 70-71 10.04887937773383 1103 72-73 9.89483476615736 1103 74-75 9.673261526106732 1103 76-77 9.519131149220307 1103 78-79 9.080508530687727 1103 80-81 8.808805832061545 1103 82-83 8.743051311342422 1103 84-85 8.466983713500781 1103 86-87 8.232862851370236 1103 88-89 7.894485767951517 1103 90-91 7.756699769789115 1103 92-93 7.379983980548028 1103 94-95 7.1451953721617 1103 96-97 6.8191142664019235 1103 98-99 6.240583113776406 1103 100-101 5.711628921130686 1104 1 7.986667668638194 1104 2 9.620115887642182 1104 3 8.783190961910321 1104 4 11.052354293276505 1104 5 11.73116523380558 1104 6 10.750293591978291 1104 7 10.39166316341236 1104 8 9.877271283129868 1104 9 9.819643935914375 1104 10-11 9.07347731710874 1104 12-13 8.80346250063873 1104 14-15 9.509217430824926 1104 16-17 10.556000601629293 1104 18-19 11.535124793223346 1104 20-21 11.74639747310366 1104 22-23 11.893085230218265 1104 24-25 11.842428419627485 1104 26-27 11.895844821827371 1104 28-29 11.76098577802934 1104 30-31 11.600752469738666 1104 32-33 11.757583303288943 1104 34-35 11.581796786809708 1104 36-37 11.574754475789494 1104 38-39 11.035522250828716 1104 40-41 10.907144789373245 1104 42-43 10.572112174781783 1104 44-45 10.80575551120862 1104 46-47 10.665152548473042 1104 48-49 10.51640775925084 1104 50-51 12.37948427611731 1104 52-53 11.414565505183582 1104 54-55 10.942165453908832 1104 56-57 10.805365898005594 1104 58-59 10.810271445631592 1104 60-61 10.63875166297105 1104 62-63 10.791480658864202 1104 64-65 10.675259637262709 1104 66-67 10.318190539688786 1104 68-69 10.050219224652853 1104 70-71 9.854348253841273 1104 72-73 9.92928116954339 1104 74-75 9.520796535450266 1104 76-77 9.239107767375291 1104 78-79 9.086301092155328 1104 80-81 8.822047296795994 1104 82-83 8.609563683480362 1104 84-85 8.214381378550208 1104 86-87 8.07149842996845 1104 88-89 7.76143876255337 1104 90-91 7.223936611124046 1104 92-93 6.991835874527654 1104 94-95 6.543008457352519 1104 96-97 6.134556041649061 1104 98-99 5.837971899467732 1104 100-101 5.090215896046288 1105 1 7.897455133612397 1105 2 9.217708684290184 1105 3 8.641228143122753 1105 4 10.74102927150317 1105 5 10.857011105853747 1105 6 10.569519300607482 1105 7 9.991496782512378 1105 8 9.63526940867153 1105 9 9.554137551475048 1105 10-11 8.826769448179675 1105 12-13 8.568406975290507 1105 14-15 9.536267158504515 1105 16-17 10.650933282056599 1105 18-19 11.123654589144763 1105 20-21 11.64666677278014 1105 22-23 12.061484140188124 1105 24-25 11.958405316634394 1105 26-27 12.055500915394173 1105 28-29 11.653604804262667 1105 30-31 11.22375651377827 1105 32-33 11.123078687448643 1105 34-35 11.214875710273962 1105 36-37 11.412846265437413 1105 38-39 11.385102048166939 1105 40-41 10.895520180397764 1105 42-43 10.49311439515414 1105 44-45 10.59728002966904 1105 46-47 10.489553601292027 1105 48-49 10.38216979660421 1105 50-51 11.861906083242708 1105 52-53 10.961058861977051 1105 54-55 10.841065399214852 1105 56-57 10.688029871675493 1105 58-59 10.881022624385732 1105 60-61 10.581962151383468 1105 62-63 10.657880792631882 1105 64-65 10.368196455292154 1105 66-67 10.348524980267765 1105 68-69 10.053501130676512 1105 70-71 10.013231053146452 1105 72-73 9.871743892897186 1105 74-75 9.609599266620542 1105 76-77 9.299185400211353 1105 78-79 8.956170992128971 1105 80-81 8.583329934745915 1105 82-83 8.514267178495084 1105 84-85 8.38019067834432 1105 86-87 8.235013509841437 1105 88-89 7.828233516315523 1105 90-91 7.58888692675054 1105 92-93 7.163122524973248 1105 94-95 6.917232088893675 1105 96-97 6.628647033609637 1105 98-99 6.1605111917180295 1105 100-101 5.462983160069573 1106 1 7.907016974519141 1106 2 9.110717639663562 1106 3 8.827768946257915 1106 4 10.593289086886632 1106 5 11.230831428697936 1106 6 10.433842733260633 1106 7 9.676816042668946 1106 8 9.304318123273394 1106 9 8.799834370924582 1106 10-11 8.446457575188845 1106 12-13 8.184926994196644 1106 14-15 8.849961116992404 1106 16-17 10.025858549606596 1106 18-19 11.317841578342602 1106 20-21 11.354635051789632 1106 22-23 11.656752754437218 1106 24-25 11.18491741624845 1106 26-27 11.498760204911594 1106 28-29 11.343815322835443 1106 30-31 10.931232184648735 1106 32-33 11.187137700260369 1106 34-35 11.13243838380193 1106 36-37 11.207455669048986 1106 38-39 10.94446230709336 1106 40-41 10.705617590820552 1106 42-43 10.546263644073456 1106 44-45 10.052626021349088 1106 46-47 10.085843428601375 1106 48-49 10.401924595914323 1106 50-51 12.118436760326531 1106 52-53 11.136454748327385 1106 54-55 10.578256805061358 1106 56-57 10.52684136748265 1106 58-59 10.237440850652117 1106 60-61 10.267008305518555 1106 62-63 10.319416744026835 1106 64-65 10.285601447263307 1106 66-67 9.973621623802583 1106 68-69 9.846958637257664 1106 70-71 9.374216958058604 1106 72-73 9.151900979724555 1106 74-75 8.91677311229764 1106 76-77 8.484454186618965 1106 78-79 8.285603369825989 1106 80-81 7.996196984543552 1106 82-83 7.955482085545134 1106 84-85 7.68959045547728 1106 86-87 7.4961122568404015 1106 88-89 7.237014931333379 1106 90-91 6.9496595898550915 1106 92-93 6.752781118970242 1106 94-95 6.50513075325655 1106 96-97 5.89604365241623 1106 98-99 5.671962863690304 1106 100-101 5.245604852669574 1107 1 7.504317712455389 1107 2 8.758933539715255 1107 3 8.415051013517981 1107 4 10.282877883536766 1107 5 11.2449209834263 1107 6 10.09955068863669 1107 7 10.091599315694346 1107 8 8.53641988435955 1107 9 5.982680716688506 1107 10-11 7.966647358324181 1107 12-13 8.054528096340903 1107 14-15 8.444117620217867 1107 16-17 9.493101662581893 1107 18-19 10.950659678119393 1107 20-21 11.134461712696957 1107 22-23 10.748655873061711 1107 24-25 10.932954162782803 1107 26-27 10.887705480868593 1107 28-29 10.917770831078311 1107 30-31 10.557523022327153 1107 32-33 11.13041204747515 1107 34-35 11.018718977392528 1107 36-37 11.301755959298145 1107 38-39 10.999529393279815 1107 40-41 10.591321266024746 1107 42-43 10.61871361759776 1107 44-45 9.920989689613737 1107 46-47 10.089787245051241 1107 48-49 10.508069120952932 1107 50-51 12.103168191343773 1107 52-53 11.037450793102387 1107 54-55 10.688065243766385 1107 56-57 10.348124718794278 1107 58-59 10.41679775482898 1107 60-61 10.375340517667166 1107 62-63 10.432915650773378 1107 64-65 10.24427752873449 1107 66-67 10.197561356779655 1107 68-69 9.992387114720277 1107 70-71 9.564724536087159 1107 72-73 9.302932233633946 1107 74-75 9.429913790689145 1107 76-77 9.118240045843802 1107 78-79 8.985015711670098 1107 80-81 8.706008806472667 1107 82-83 8.518257415075093 1107 84-85 8.389348987693051 1107 86-87 8.082945260112687 1107 88-89 8.016562672952618 1107 90-91 7.590516584120131 1107 92-93 7.29251649577092 1107 94-95 7.098069689208968 1107 96-97 6.882835506242065 1107 98-99 6.634192117248791 1107 100-101 6.24638142061691 1108 1 6.5987360930900785 1108 2 8.108521969365764 1108 3 7.871600957921796 1108 4 9.31355310468274 1108 5 11.104530799275079 1108 6 9.666690071548743 1108 7 9.10995537310211 1108 8 6.172117988143061 1108 9 7.434534022035454 1108 10-11 6.420670401506861 1108 12-13 5.203862230417897 1108 14-15 7.44384409886834 1108 16-17 8.715394048976378 1108 18-19 8.642523607194114 1108 20-21 8.711086142117548 1108 22-23 9.70558557609284 1108 24-25 9.369842877252278 1108 26-27 8.075172455147683 1108 28-29 9.941783428916704 1108 30-31 10.393319237029516 1108 32-33 10.767374691990543 1108 34-35 10.180140773080009 1108 36-37 10.029423040210911 1108 38-39 9.633137437185937 1108 40-41 10.02330978605622 1108 42-43 8.990490971219241 1108 44-45 8.51984526043099 1108 46-47 9.418340297291838 1108 48-49 9.895061595151745 1108 50-51 11.574761215360134 1108 52-53 10.721314817314976 1108 54-55 9.955754444231768 1108 56-57 9.787551433901974 1108 58-59 9.937611936712752 1108 60-61 9.824592198931544 1108 62-63 9.899187891725862 1108 64-65 9.702782952583568 1108 66-67 9.13644043457666 1108 68-69 9.39317893920678 1108 70-71 9.507016684637001 1108 72-73 9.296011747083071 1108 74-75 9.145261509659836 1108 76-77 8.702296724224315 1108 78-79 8.623728298210867 1108 80-81 8.384133639238144 1108 82-83 8.506362605836417 1108 84-85 8.106729485620829 1108 86-87 7.80810862965787 1108 88-89 7.513880974503964 1108 90-91 7.3496473411994785 1108 92-93 7.0156655193195245 1108 94-95 6.865984247481903 1108 96-97 6.42428845957893 1108 98-99 6.171909720329673 1108 100-101 5.419373764001161 1109 1 6.5734740018623405 1109 2 8.29115372088263 1109 3 7.964824091220692 1109 4 9.183493182094569 1109 5 10.195828994472436 1109 6 8.990798110332506 1109 7 7.7844174519270695 1109 8 8.472675915400746 1109 9 7.647133532917248 1109 10-11 5.916750792643093 1109 12-13 6.617377040196828 1109 14-15 7.837242190555244 1109 16-17 6.827075754015802 1109 18-19 5.910919670234549 1109 20-21 8.364953797840077 1109 22-23 9.575859539025675 1109 24-25 9.289073983956445 1109 26-27 9.431337396310337 1109 28-29 9.657368249759184 1109 30-31 7.458916163778561 1109 32-33 6.269617352413604 1109 34-35 8.3415968200628 1109 36-37 6.776107506188698 1109 38-39 7.154997933600672 1109 40-41 7.971520651816558 1109 42-43 6.863247139788879 1109 44-45 7.196027895349179 1109 46-47 6.813452809653388 1109 48-49 7.653331182805438 1109 50-51 9.54932020740982 1109 52-53 9.777616584909275 1109 54-55 9.84491579848358 1109 56-57 8.748857987782497 1109 58-59 8.490365915003924 1109 60-61 8.676294777588605 1109 62-63 9.155610652413532 1109 64-65 9.19410278667657 1109 66-67 8.873376512091404 1109 68-69 8.493090536401375 1109 70-71 7.885068379167041 1109 72-73 7.350740604463617 1109 74-75 6.55189501867725 1109 76-77 6.495128180317476 1109 78-79 7.317169997550284 1109 80-81 7.294690121703713 1109 82-83 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52-53 -17.313111412193223 2313 54-55 -18.170839362689907 2313 56-57 -18.17073636209074 2313 58-59 -17.927779684416574 2313 60-61 -17.640981571560257 2313 62-63 -17.22685946710838 2313 64-65 -16.88883817674248 2313 66-67 -16.571027689284907 2313 68-69 -16.147076069900688 2313 70-71 -15.81793777802238 2313 72-73 -15.447339801186509 2313 74-75 -14.997543590522316 2313 76-77 -14.609725133699182 2313 78-79 -14.245488459530481 2313 80-81 -13.831713355297374 2313 82-83 -13.385804971577064 2313 84-85 -13.003106579952942 2313 86-87 -12.548175523347595 2313 88-89 -12.119998806916803 2313 90-91 -11.643977431113818 2313 92-93 -11.086155974305248 2313 94-95 -10.520718849057262 2313 96-97 -9.891194236231636 2313 98-99 -9.202042920836085 2313 100-101 -8.158950461864048 2314 1 -19.51055352439626 2314 2 -18.12608640950491 2314 3 -18.854082102187494 2314 4 -20.31741228693839 2314 5 -19.64082439198175 2314 6 -20.716916485828307 2314 7 -20.940682149666557 2314 8 -21.462854689452566 2314 9 -22.878762881425384 2314 10-11 -23.349457103046873 2314 12-13 -23.313988407104873 2314 14-15 -23.326647704410348 2314 16-17 -22.10628172515841 2314 18-19 -20.979880764390586 2314 20-21 -20.643376517263146 2314 22-23 -20.27401369530971 2314 24-25 -20.130339272110195 2314 26-27 -19.870364885471627 2314 28-29 -19.70011092445306 2314 30-31 -19.755861090839332 2314 32-33 -19.658126218756262 2314 34-35 -19.36485011945187 2314 36-37 -18.90208879949765 2314 38-39 -18.813310650245757 2314 40-41 -18.72605269117511 2314 42-43 -18.592022689982944 2314 44-45 -18.23388880149979 2314 46-47 -17.900056788318363 2314 48-49 -17.724792685358274 2314 50-51 -15.315402719066093 2314 52-53 -17.313111412193223 2314 54-55 -18.170839362689907 2314 56-57 -18.17073636209074 2314 58-59 -17.927779684416574 2314 60-61 -17.640981571560257 2314 62-63 -17.22685946710838 2314 64-65 -16.88883817674248 2314 66-67 -16.571027689284907 2314 68-69 -16.147076069900688 2314 70-71 -15.81793777802238 2314 72-73 -15.447339801186509 2314 74-75 -14.997543590522316 2314 76-77 -14.609725133699182 2314 78-79 -14.245488459530481 2314 80-81 -13.831713355297374 2314 82-83 -13.385804971577064 2314 84-85 -13.003106579952942 2314 86-87 -12.548175523347595 2314 88-89 -12.119998806916803 2314 90-91 -11.643977431113818 2314 92-93 -11.086155974305248 2314 94-95 -10.520718849057262 2314 96-97 -9.891194236231636 2314 98-99 -9.202042920836085 2314 100-101 -8.158950461864048 2315 1 -19.51055352439626 2315 2 -18.12608640950491 2315 3 -18.854082102187494 2315 4 -20.31741228693839 2315 5 -19.64082439198175 2315 6 -20.716916485828307 2315 7 -20.940682149666557 2315 8 -21.462854689452566 2315 9 -22.878762881425384 2315 10-11 -23.349457103046873 2315 12-13 -23.313988407104873 2315 14-15 -23.326647704410348 2315 16-17 -22.10628172515841 2315 18-19 -20.979880764390586 2315 20-21 -20.643376517263146 2315 22-23 -20.27401369530971 2315 24-25 -20.130339272110195 2315 26-27 -19.870364885471627 2315 28-29 -19.70011092445306 2315 30-31 -19.755861090839332 2315 32-33 -19.658126218756262 2315 34-35 -19.36485011945187 2315 36-37 -18.90208879949765 2315 38-39 -18.813310650245757 2315 40-41 -18.72605269117511 2315 42-43 -18.592022689982944 2315 44-45 -18.23388880149979 2315 46-47 -17.900056788318363 2315 48-49 -17.724792685358274 2315 50-51 -15.315402719066093 2315 52-53 -17.313111412193223 2315 54-55 -18.170839362689907 2315 56-57 -18.17073636209074 2315 58-59 -17.927779684416574 2315 60-61 -17.640981571560257 2315 62-63 -17.22685946710838 2315 64-65 -16.88883817674248 2315 66-67 -16.571027689284907 2315 68-69 -16.147076069900688 2315 70-71 -15.81793777802238 2315 72-73 -15.447339801186509 2315 74-75 -14.997543590522316 2315 76-77 -14.609725133699182 2315 78-79 -14.245488459530481 2315 80-81 -13.831713355297374 2315 82-83 -13.385804971577064 2315 84-85 -13.003106579952942 2315 86-87 -12.548175523347595 2315 88-89 -12.119998806916803 2315 90-91 -11.643977431113818 2315 92-93 -11.086155974305248 2315 94-95 -10.520718849057262 2315 96-97 -9.891194236231636 2315 98-99 -9.202042920836085 2315 100-101 -8.158950461864048 2316 1 -19.51055352439626 2316 2 -18.12608640950491 2316 3 -18.854082102187494 2316 4 -20.31741228693839 2316 5 -19.64082439198175 2316 6 -20.716916485828307 2316 7 -20.940682149666557 2316 8 -21.462854689452566 2316 9 -22.878762881425384 2316 10-11 -23.349457103046873 2316 12-13 -23.313988407104873 2316 14-15 -23.326647704410348 2316 16-17 -22.10628172515841 2316 18-19 -20.979880764390586 2316 20-21 -20.643376517263146 2316 22-23 -20.27401369530971 2316 24-25 -20.130339272110195 2316 26-27 -19.870364885471627 2316 28-29 -19.70011092445306 2316 30-31 -19.755861090839332 2316 32-33 -19.658126218756262 2316 34-35 -19.36485011945187 2316 36-37 -18.90208879949765 2316 38-39 -18.813310650245757 2316 40-41 -18.72605269117511 2316 42-43 -18.592022689982944 2316 44-45 -18.23388880149979 2316 46-47 -17.900056788318363 2316 48-49 -17.724792685358274 2316 50-51 -15.315402719066093 2316 52-53 -17.313111412193223 2316 54-55 -18.170839362689907 2316 56-57 -18.17073636209074 2316 58-59 -17.927779684416574 2316 60-61 -17.640981571560257 2316 62-63 -17.22685946710838 2316 64-65 -16.88883817674248 2316 66-67 -16.571027689284907 2316 68-69 -16.147076069900688 2316 70-71 -15.81793777802238 2316 72-73 -15.447339801186509 2316 74-75 -14.997543590522316 2316 76-77 -14.609725133699182 2316 78-79 -14.245488459530481 2316 80-81 -13.831713355297374 2316 82-83 -13.385804971577064 2316 84-85 -13.003106579952942 2316 86-87 -12.548175523347595 2316 88-89 -12.119998806916803 2316 90-91 -11.643977431113818 2316 92-93 -11.086155974305248 2316 94-95 -10.520718849057262 2316 96-97 -9.891194236231636 2316 98-99 -9.202042920836085 2316 100-101 -8.158950461864048 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 629090.0 3 22915.0 4 100108.0 5 48291.0 6 24593.0 7 25457.0 8 24213.0 9 22356.0 10 23701.0 11 26030.0 12 27756.0 13 29322.0 14 30510.0 15 31875.0 16 33707.0 17 34327.0 18 32990.0 19 33669.0 20 36065.0 21 38735.0 22 41569.0 23 44920.0 24 48835.0 25 52792.0 26 57965.0 27 63503.0 28 69045.0 29 76219.0 30 85171.0 31 93937.0 32 105064.0 33 117292.0 34 128268.0 35 133513.0 36 116677.0 37 64870.0 38 12267.0 39 146.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.58346982136585 21.05114910752475 14.4259032676154 22.939477803493993 2 14.329118126626572 20.985624466910497 42.54597538587216 22.13928202059077 3 18.165073873251565 26.651198071479165 30.885951908465543 24.297776146803727 4 11.696563340843962 17.736311333756262 41.12385973355501 29.44326559184477 5 11.992241814696968 38.55630489828392 36.61012373401847 12.841329553000635 6 28.389726482396092 39.82545665350448 18.734862457243533 13.049954406855887 7 25.011630348625737 33.553263437692095 24.005547994063566 17.429558219618606 8 23.56688421323236 37.461305994562785 21.34372454023838 17.62808525196647 9 24.70752939175058 17.285120368722108 22.270910081017494 35.73644015850982 10-11 23.08855725215627 27.900796193418888 29.933424162222412 19.077222392202437 12-13 23.813397079909414 25.42961995795747 30.03214411181436 20.724838850318754 14-15 21.08395344583602 27.22756765082452 27.84833797013833 23.84014093320113 16-17 20.419826967043004 30.382665273887678 28.363838657549838 20.83366910151948 18-19 20.136679961243406 29.188201781935426 30.58894780930529 20.08617044751588 20-21 21.043271063667557 28.426665214312948 30.603289581177496 19.926774140842003 22-23 20.58974287461946 28.306997290353014 30.214554110488717 20.88870572453881 24-25 20.488088786245424 28.468123181710503 30.548908223848 20.494879808196075 26-27 20.52789463868934 28.669317589116766 30.231207317347142 20.571580454846757 28-29 20.422733543670482 28.724492510794814 30.386277123304207 20.4664968222305 30-31 20.735787467466956 28.45694558525389 30.364183661620114 20.44308328565904 32-33 20.576146928761773 28.686280458574497 30.18961049536185 20.54796211730188 34-35 20.65786368966486 28.689181172657673 30.30411525013909 20.348839887538368 36-37 20.77146267511443 28.470849341639003 30.19324533871887 20.56444264452769 38-39 20.60106613633985 28.781035119910968 30.211726645291247 20.406172098457937 40-41 20.84111186104096 28.722256299581705 30.12528900222175 20.311342837155582 42-43 20.709622532376414 28.76127954599098 29.84403814879274 20.685059772839864 44-45 20.76756157811929 28.995569592982328 29.771101161512647 20.465767667385734 46-47 20.794918855564585 28.81518224552238 29.50836841548424 20.881530483428797 48-49 20.814051525535195 28.956421443508983 29.454799365605133 20.774727665350692 50-51 21.097073095328433 29.220349646040944 28.92889687355084 20.753680385079782 52-53 20.59229654406885 29.377076321331465 29.142896538315416 20.88773059628427 54-55 20.593098262334884 29.248858971493917 29.3327122353287 20.825330530842496 56-57 20.65244309696592 29.389077608914544 29.230602817507894 20.72787647661164 58-59 20.559681280783252 29.29672228940109 29.37616624834043 20.767430181475227 60-61 20.63730938044234 29.38776820213474 29.290387198384437 20.684535219038484 62-63 20.555691843844972 29.297180357708037 29.229152022737125 20.91797577570987 64-65 20.414523920835283 29.23125448028674 29.323883434626772 21.03033816425121 66-67 20.426219574794505 29.303279768689492 29.085127195425397 21.185373461090602 68-69 20.517847034716567 29.293365957410018 29.086549586850413 21.102237421023 70-71 20.551029720894395 29.032992461929375 29.20841530953966 21.207562507636567 72-73 20.579971792097755 29.200778591859727 29.097220200083473 21.12202941595904 74-75 20.435415067456113 29.40388005866732 28.814873421908448 21.345831451968113 76-77 20.4820783083325 29.350674680926264 28.95353419145711 21.213712819284122 78-79 20.381828801495956 29.397812884703335 28.87832956873097 21.34202874506974 80-81 20.419158172652065 29.34508891068664 28.78853548542587 21.447217431235426 82-83 20.587771522572012 29.213069934492896 28.98349048503735 21.21566805789774 84-85 20.697280728644227 29.275815254420834 28.765374542263906 21.261529474671033 86-87 20.404848421646026 29.41186742741559 28.91051689967431 21.272767251264074 88-89 20.59090055318917 29.305841507970648 28.779917960773858 21.323339978066322 90-91 20.480676899748804 29.289341798677494 28.94435755442481 21.285623747148897 92-93 20.467884831071352 29.29723401515546 28.66093168049872 21.573949473274467 94-95 20.22588204202302 29.331800442038176 28.80506135896983 21.637256156968977 96-97 20.33647100765922 29.18765477532182 28.7755355321971 21.70033868482186 98-99 20.207326102638884 29.240001702981477 28.507291543531508 22.045380650848127 100-101 20.3650744592337 29.36284591428976 28.497454738978124 21.77462488749842 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 155827.0 1 141977.0 2 94692.0 3 47824.0 4 29703.0 5 23539.5 6 22002.5 7 22288.5 8 22790.0 9 22318.0 10 20079.5 11 16634.0 12 13084.0 13 10016.0 14 7804.5 15 6531.5 16 6326.5 17 6947.0 18 8045.5 19 9410.5 20 10801.0 21 12364.5 22 14158.0 23 16128.0 24 18266.0 25 20787.0 26 24214.0 27 28432.0 28 32961.5 29 37905.5 30 42933.5 31 48140.5 32 53646.0 33 58843.0 34 63897.0 35 68183.5 36 72404.5 37 75843.0 38 77332.0 39 77684.5 40 77453.0 41 76636.0 42 75537.0 43 75547.0 44 75351.0 45 74820.5 46 73286.5 47 70192.5 48 67237.0 49 63984.5 50 59525.0 51 54616.5 52 49603.5 53 44219.0 54 38672.0 55 32935.0 56 27293.5 57 22003.0 58 17504.0 59 13801.5 60 10368.0 61 7466.0 62 5294.5 63 3546.5 64 2243.0 65 1424.5 66 901.0 67 532.0 68 336.0 69 204.5 70 109.5 71 73.5 72 48.5 73 36.0 74 32.5 75 23.0 76 14.5 77 7.5 78 6.5 79 7.0 80 5.0 81 4.0 82 2.5 83 1.5 84 0.5 85 1.0 86 1.5 87 0.5 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 22.21092117013807 2 29.23208191785724 3 23.022587462607667 4 29.339742472552548 5 32.43790099788891 6 25.074746025814576 7 23.99470121491033 8 20.029693600225368 9 11.864339972400872 10-11 15.505052046883739 12-13 15.576020678864333 14-15 19.715213487479343 16-17 23.033852018133036 18-19 26.97387666490324 20-21 29.046825385477725 22-23 28.64298237512477 24-25 27.163287364414746 26-27 28.614734038627187 28-29 25.56225203003521 30-31 27.453866524871096 32-33 26.441969376639207 34-35 24.501084527447066 36-37 27.480607768176608 38-39 27.638041041625527 40-41 24.652605358373236 42-43 25.506508903636078 44-45 24.216147305607198 46-47 27.49755290573364 48-49 22.907468728782348 50-51 28.5014122236078 52-53 22.087687319124665 54-55 23.22310814398382 56-57 25.334410454125823 58-59 24.628665762668376 60-61 24.14651187145036 62-63 23.995068327354552 64-65 22.507876494099342 66-67 23.024075234092148 68-69 24.017887264019155 70-71 22.513962831990412 72-73 22.364934501343438 74-75 19.679680867220068 76-77 21.93775086822093 78-79 21.036953538635494 80-81 22.074857705284447 82-83 22.925070804397464 84-85 19.887930231632495 86-87 21.441453487046534 88-89 20.277185352754483 90-91 21.404664955793866 92-93 21.374329874876487 94-95 20.455640644062072 96-97 19.355211431649654 98-99 18.763986501082208 100-101 20.417151802541422 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2587763.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.35146646382714 #Duplication Level Percentage of deduplicated Percentage of total 1 87.49723245687095 65.93044777151599 2 8.492599175429357 12.798596039161822 3 2.004658510238437 4.531618755169717 4 0.7557739051107034 2.277946882607394 5 0.3757721492402126 1.4157491250757064 6 0.2185016534891672 0.9878652009107856 7 0.141568956776079 0.7467199949182198 8 0.09182738743992752 0.5535462644112449 9 0.07096730009070598 0.48127411195318703 >10 0.3324579953358663 4.258361240019327 >50 0.013238871716528489 0.6756007967573275 >100 0.005194599770811329 0.654745330299963 >500 5.1759622821428285E-5 0.03787058742196129 >1k 1.0351924564285657E-4 0.15495349518790133 >5k 0.0 0.0 >10k+ 5.1759622821428285E-5 4.494704404589606 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 115244 4.453421739162358 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 3.8643415181374804E-5 0.0 3 0.0 0.0 0.0 3.8643415181374804E-5 0.0 4 0.0 0.0 0.0 3.8643415181374804E-5 0.0 5 0.0 0.0 0.0 3.8643415181374804E-5 0.0 6 7.728683036274961E-5 0.0 0.0 3.8643415181374804E-5 0.0 7 7.728683036274961E-5 0.0 0.0 3.8643415181374804E-5 0.0 8 7.728683036274961E-5 0.0 0.0 3.8643415181374804E-5 0.0 9 7.728683036274961E-5 0.0 0.0 3.8643415181374804E-5 0.0 10-11 7.728683036274961E-5 0.0 0.0 9.660853795343701E-5 0.0 12-13 7.728683036274961E-5 0.0 0.0 1.159302455441244E-4 5.79651227720622E-5 14-15 7.728683036274961E-5 0.0 0.0 1.159302455441244E-4 7.728683036274961E-5 16-17 7.728683036274961E-5 0.0 0.0 1.5457366072549921E-4 7.728683036274961E-5 18-19 7.728683036274961E-5 0.0 0.0 1.93217075906874E-4 7.728683036274961E-5 20-21 7.728683036274961E-5 0.0 0.0 2.318604910882488E-4 7.728683036274961E-5 22-23 7.728683036274961E-5 0.0 0.0 2.89825613860311E-4 7.728683036274961E-5 24-25 7.728683036274961E-5 1.9321707590687402E-5 0.0 3.0914732145099843E-4 7.728683036274961E-5 26-27 7.728683036274961E-5 3.8643415181374804E-5 0.0 3.284690290416858E-4 1.159302455441244E-4 28-29 7.728683036274961E-5 3.8643415181374804E-5 0.0 4.8304268976718503E-4 1.159302455441244E-4 30-31 7.728683036274961E-5 3.8643415181374804E-5 0.0 0.0011013373326691819 1.159302455441244E-4 32-33 7.728683036274961E-5 3.8643415181374804E-5 0.0 0.002859612723421735 1.159302455441244E-4 34-35 7.728683036274961E-5 3.8643415181374804E-5 0.0 0.005545330078527284 1.5457366072549921E-4 36-37 7.728683036274961E-5 3.8643415181374804E-5 0.0 0.010433722098971197 1.5457366072549921E-4 38-39 7.728683036274961E-5 3.8643415181374804E-5 0.0 0.017370215124027973 1.5457366072549921E-4 40-41 7.728683036274961E-5 3.8643415181374804E-5 0.0 0.025079576452712245 1.5457366072549921E-4 42-43 7.728683036274961E-5 3.8643415181374804E-5 0.0 0.03365841462297745 1.5457366072549921E-4 44-45 7.728683036274961E-5 3.8643415181374804E-5 0.0 0.04503890039389233 1.5457366072549921E-4 46-47 7.728683036274961E-5 3.8643415181374804E-5 0.0 0.05879595619846176 1.5457366072549921E-4 48-49 7.728683036274961E-5 3.8643415181374804E-5 0.0 0.0742146788558303 1.5457366072549921E-4 50-51 7.728683036274961E-5 3.8643415181374804E-5 0.0 0.09030966127887291 1.5457366072549921E-4 52-53 7.728683036274961E-5 3.8643415181374804E-5 0.0 0.10899375251906762 1.5457366072549921E-4 54-55 7.728683036274961E-5 3.8643415181374804E-5 0.0 0.1289723981678384 1.5457366072549921E-4 56-57 7.728683036274961E-5 3.8643415181374804E-5 0.0 0.15277674191956525 1.5457366072549921E-4 58-59 7.728683036274961E-5 3.8643415181374804E-5 0.0 0.18060000085015515 1.5457366072549921E-4 60-61 7.728683036274961E-5 3.8643415181374804E-5 0.0 0.21369808595300266 1.5457366072549921E-4 62-63 7.728683036274961E-5 3.8643415181374804E-5 0.0 0.24805208204924484 1.5457366072549921E-4 64-65 7.728683036274961E-5 3.8643415181374804E-5 0.0 0.2825413300986218 1.5457366072549921E-4 66-67 7.728683036274961E-5 3.8643415181374804E-5 0.0 0.32249862139616337 1.5457366072549921E-4 68-69 7.728683036274961E-5 3.8643415181374804E-5 0.0 0.3664941495801586 1.5457366072549921E-4 70-71 7.728683036274961E-5 3.8643415181374804E-5 0.0 0.41947427179382346 1.5457366072549921E-4 72-73 7.728683036274961E-5 3.8643415181374804E-5 0.0 0.4757777277130866 1.5457366072549921E-4 74-75 7.728683036274961E-5 3.8643415181374804E-5 0.0 0.5315401758198104 1.5457366072549921E-4 76-77 7.728683036274961E-5 3.8643415181374804E-5 0.0 0.5945289425654513 1.5457366072549921E-4 78-79 7.728683036274961E-5 3.8643415181374804E-5 0.0 0.6677582143341566 1.5457366072549921E-4 80-81 7.728683036274961E-5 3.8643415181374804E-5 0.0 0.7503391925767545 1.5457366072549921E-4 82-83 7.728683036274961E-5 3.8643415181374804E-5 0.0 0.8374800938107547 1.5457366072549921E-4 84-85 7.728683036274961E-5 3.8643415181374804E-5 0.0 0.9235389794196764 1.5457366072549921E-4 86-87 7.728683036274961E-5 3.8643415181374804E-5 0.0 1.019297362239123 1.5457366072549921E-4 88-89 7.728683036274961E-5 3.8643415181374804E-5 0.0 1.1309768321132962 1.5457366072549921E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2170 0.0 55.720646 1 GTATCAA 4305 0.0 47.961834 1 ATCAACG 4875 0.0 41.5391 3 TCAACGC 5085 0.0 40.314262 4 CAACGCA 5165 0.0 39.583134 5 TATCAAC 5170 0.0 39.137543 2 AACGCAG 5515 0.0 36.756897 6 ACGCAGA 6175 0.0 32.25741 7 CGCAGAG 6230 0.0 31.132236 8 GCAGAGT 6980 0.0 26.19588 9 GAGTACT 4585 0.0 23.042437 12-13 CAGAGTA 6585 0.0 22.120644 10-11 GTGTAGC 950 0.0 21.572489 1 AGAGTAC 6190 0.0 21.4232 10-11 GTACATG 4050 0.0 20.746874 1 GTGGTAT 1250 0.0 20.493866 1 GTACTTT 5280 0.0 19.84084 14-15 TACATGG 4080 0.0 19.711807 2 ACATGGG 4110 0.0 19.120026 3 AGTACTT 5250 0.0 19.02607 12-13 >>END_MODULE