FastQCFastQC Report
Sat 18 Jun 2016
SRR1931005_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931005_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2587763
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67530.26095898271982404No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58100.2245182422037876No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34170.1320445496747577No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34600.057.5506251
GTATCAA66600.043.599261
ATCAACG80700.035.50633
TCAACGC81950.035.02274
CAACGCA82900.034.6230625
AACGCAG86000.033.595036
TATCAAC86100.033.4482352
ACGCAGA97050.029.4277577
CGCAGAG97800.029.1535768
GCAGAGT112600.025.0262579
GTGGTAT18000.022.9698871
GAGTACT84100.021.74584212-13
AGAGTAC104600.021.08963610-11
CAGAGTA111650.020.65025310-11
TGGTATC19200.020.048762
GTACTTT94250.019.55498714-15
AGTACTT93700.018.78374312-13
GTACATG61100.018.5895481
GTGTAGC16950.018.5048831
ACATGGG55900.018.1912963
Produced by FastQC (version 0.11.5)