##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931005_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2587763 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.350915056749788 31.0 31.0 34.0 30.0 34.0 2 31.510484151755783 31.0 31.0 34.0 30.0 34.0 3 31.574118649969105 31.0 31.0 34.0 30.0 34.0 4 35.35007340316714 37.0 35.0 37.0 33.0 37.0 5 35.23289149740529 37.0 35.0 37.0 33.0 37.0 6 35.25953342713378 37.0 35.0 37.0 32.0 37.0 7 35.144902373208055 37.0 35.0 37.0 32.0 37.0 8 35.14562809654516 37.0 35.0 37.0 32.0 37.0 9 36.65090002446128 39.0 35.0 39.0 32.0 39.0 10-11 36.590821300095875 38.5 35.0 39.0 32.0 39.0 12-13 36.485469496240576 38.0 35.0 39.0 32.0 39.0 14-15 37.55051486554217 39.0 36.0 41.0 32.0 41.0 16-17 37.507546672550774 39.0 36.0 41.0 32.0 41.0 18-19 37.541686197692755 39.0 36.0 41.0 32.0 41.0 20-21 37.52728862728156 39.0 36.0 41.0 32.0 41.0 22-23 37.428801246481996 39.0 36.0 41.0 32.0 41.0 24-25 37.308716254154646 39.0 36.0 41.0 31.5 41.0 26-27 37.05797826153322 39.0 36.0 40.0 31.0 41.0 28-29 36.87728377753295 39.0 36.0 40.0 30.5 41.0 30-31 36.64692265095374 38.5 35.5 40.0 30.0 41.0 32-33 36.47496235165276 38.0 35.0 40.0 30.0 41.0 34-35 36.54717800664126 38.0 35.0 40.0 30.0 41.0 36-37 36.6073438719079 39.0 35.0 40.0 30.0 41.0 38-39 36.58377216151557 39.0 35.0 40.0 30.0 41.0 40-41 36.466770913719685 39.0 35.0 40.0 30.0 41.0 42-43 36.319052981281516 39.0 35.0 40.0 30.0 41.0 44-45 36.14723914052407 38.5 35.0 40.0 30.0 41.0 46-47 35.90457800810971 38.0 35.0 40.0 29.0 41.0 48-49 35.69041330291839 38.0 35.0 40.0 28.0 41.0 50-51 35.42456670104642 38.0 34.5 40.0 27.5 41.0 52-53 35.216999392911944 38.0 34.0 40.0 27.0 41.0 54-55 35.07761452652349 38.0 34.0 40.0 26.0 41.0 56-57 34.91879936454768 38.0 34.0 40.0 26.0 41.0 58-59 34.72470063912344 38.0 33.5 40.0 26.0 41.0 60-61 34.43433517675305 38.0 33.0 40.0 25.5 41.0 62-63 34.19334942960387 37.0 33.0 40.0 25.0 41.0 64-65 33.89404130130928 37.0 32.5 40.0 24.5 41.0 66-67 33.588419611842355 36.5 32.0 39.0 23.0 41.0 68-69 33.25324208592518 36.0 32.0 39.0 23.0 40.5 70-71 32.88743095870835 36.0 31.5 39.0 22.0 40.0 72-73 32.46449095222398 35.0 31.0 38.5 22.0 40.0 74-75 31.942863778483577 35.0 31.0 37.5 20.0 39.5 76-77 30.57195867627754 33.5 29.5 36.0 20.0 39.0 78-79 31.29892208057693 34.0 30.0 36.0 20.0 39.0 80-81 31.2644324460934 34.0 31.0 36.0 20.0 39.0 82-83 30.9823376406572 34.0 30.5 36.0 20.0 37.5 84-85 30.62993944963275 34.0 30.0 35.5 19.0 37.0 86-87 30.268142213950814 34.0 30.0 35.0 18.0 37.0 88-89 29.90457878097801 34.0 30.0 35.0 15.0 36.0 90-91 29.561019111873847 34.0 29.0 35.0 9.0 36.0 92-93 29.23737857755907 34.0 29.0 35.0 4.5 35.5 94-95 28.84803457658217 34.0 29.0 35.0 2.0 35.0 96-97 28.41807093617151 34.0 29.0 35.0 2.0 35.0 98-99 27.922537728532326 34.0 29.0 35.0 2.0 35.0 100-101 26.78083541653544 32.5 26.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.31975282450068576 1101 2 0.3677854413664363 1101 3 0.2081451575751352 1101 4 0.13392669201406449 1101 5 0.12672353666475544 1101 6 0.303513413154441 1101 7 0.2769219955982365 1101 8 0.37652577999736536 1101 9 0.5075668870872931 1101 10-11 0.46866223569358567 1101 12-13 0.48902143790520114 1101 14-15 0.5350825536702644 1101 16-17 0.5576225330911981 1101 18-19 0.4833010623256442 1101 20-21 0.4898845192040824 1101 22-23 0.5523588728966544 1101 24-25 0.5492476924483327 1101 26-27 0.4624680447076557 1101 28-29 0.5730336499472486 1101 30-31 0.6454105280829125 1101 32-33 0.632734708594775 1101 34-35 0.636770600918652 1101 36-37 0.6453054571440902 1101 38-39 0.6521883440760234 1101 40-41 0.7249111489627396 1101 42-43 0.6765738963357535 1101 44-45 0.6276919310180418 1101 46-47 0.5769198154125661 1101 48-49 0.5882598701212203 1101 50-51 0.6758000047801644 1101 52-53 0.6245327329595369 1101 54-55 0.5342084980680042 1101 56-57 0.6238003600501543 1101 58-59 0.7302115708045065 1101 60-61 0.7033800690262737 1101 62-63 0.4681483695188433 1101 64-65 0.5347829312909695 1101 66-67 0.500032535085225 1101 68-69 0.582385889809153 1101 70-71 0.4695427105489074 1101 72-73 0.4549137662337941 1101 74-75 0.3639382619610778 1101 76-77 0.3970244611445324 1101 78-79 0.5167859034746378 1101 80-81 0.5649414589114059 1101 82-83 0.556641842674285 1101 84-85 0.5823930225252951 1101 86-87 0.5139953695961204 1101 88-89 0.48167556683663193 1101 90-91 0.5534048730448724 1101 92-93 0.5130172757311406 1101 94-95 0.7160787029373097 1101 96-97 0.8677360210644416 1101 98-99 0.8325352427215691 1101 100-101 1.1947010090620154 1102 1 0.1847487895162807 1102 2 0.3318108290520989 1102 3 0.3041865107126078 1102 4 0.13989410678864544 1102 5 0.270595813050285 1102 6 0.34477930772138876 1102 7 0.3479682561491799 1102 8 0.3636792620798559 1102 9 0.32220624672436315 1102 10-11 0.3985560174554479 1102 12-13 0.409425372560257 1102 14-15 0.49403694744111704 1102 16-17 0.41920511940714533 1102 18-19 0.4651610810828686 1102 20-21 0.5216474802283102 1102 22-23 0.4559140213186481 1102 24-25 0.5713724934254572 1102 26-27 0.5351130315121608 1102 28-29 0.3832803292641316 1102 30-31 0.38842128077109095 1102 32-33 0.5208871219516666 1102 34-35 0.4871621034595961 1102 36-37 0.4285002923640562 1102 38-39 0.3492962417400918 1102 40-41 0.4590623845403954 1102 42-43 0.4704254525310887 1102 44-45 0.4708575398644754 1102 46-47 0.4098234774335481 1102 48-49 0.43284317619114887 1102 50-51 0.38128976464406605 1102 52-53 0.3605553288722092 1102 54-55 0.5177304941856988 1102 56-57 0.49420756631420204 1102 58-59 0.6277774937253966 1102 60-61 0.3966776327681174 1102 62-63 0.19419692019606316 1102 64-65 0.43130193933912153 1102 66-67 0.280710412465325 1102 68-69 0.5206942498606253 1102 70-71 0.4550233099076735 1102 72-73 0.41110474095516025 1102 74-75 0.33771304439907723 1102 76-77 0.3744394538379403 1102 78-79 0.48264775433288776 1102 80-81 0.5393814685081253 1102 82-83 0.48863163525427566 1102 84-85 0.4999790113145828 1102 86-87 0.37121799168166447 1102 88-89 0.48489919221559674 1102 90-91 0.40507012201431536 1102 92-93 0.34263406126626705 1102 94-95 0.5094547852946114 1102 96-97 0.5822728702868893 1102 98-99 0.7006414173382396 1102 100-101 0.8094755952034802 1103 1 0.23801883038603577 1103 2 0.3534735577256285 1103 3 0.3342558712846362 1103 4 0.24073853639063714 1103 5 0.2839272380681308 1103 6 0.2851256167630325 1103 7 0.31702048456784837 1103 8 0.32094262049749744 1103 9 0.443869310190081 1103 10-11 0.36992040443839613 1103 12-13 0.4590606164689248 1103 14-15 0.48539930893131356 1103 16-17 0.5293896233832172 1103 18-19 0.5383585808547906 1103 20-21 0.5807652944370574 1103 22-23 0.5861760129471705 1103 24-25 0.5330544810197324 1103 26-27 0.48383228398815703 1103 28-29 0.5373678071155297 1103 30-31 0.4760993797540678 1103 32-33 0.5432755126976829 1103 34-35 0.5583684266796922 1103 36-37 0.6379136443787914 1103 38-39 0.61100287840938 1103 40-41 0.6363509611797653 1103 42-43 0.5283658444555712 1103 44-45 0.5157226227966802 1103 46-47 0.5944132440080665 1103 48-49 0.38319935323260523 1103 50-51 0.3438455598550334 1103 52-53 0.43780318641143623 1103 54-55 0.44318294490637555 1103 56-57 0.554719371505314 1103 58-59 0.5920735805905721 1103 60-61 0.5651057248460205 1103 62-63 0.38983650301152295 1103 64-65 0.29762590434999936 1103 66-67 0.2833812171634662 1103 68-69 0.2320297275379346 1103 70-71 0.39489798045123337 1103 72-73 0.24283630290301517 1103 74-75 0.27653458425902144 1103 76-77 0.3203085785488682 1103 78-79 0.39942490242235706 1103 80-81 0.3186732742181775 1103 82-83 0.2955812515405789 1103 84-85 0.35123314285945284 1103 86-87 0.3043627114647869 1103 88-89 0.25048042075227883 1103 90-91 0.41589939389989183 1103 92-93 0.2511872484460689 1103 94-95 0.3775657036212223 1103 96-97 0.3691889403957198 1103 98-99 0.3166580012693885 1103 100-101 0.4960134338353157 1104 1 0.2858766190327806 1104 2 0.39973571038869693 1104 3 0.3902407119761264 1104 4 0.2725444807375581 1104 5 0.3335833406683477 1104 6 0.30506012417890105 1104 7 0.2998166934025406 1104 8 0.33589297833422904 1104 9 0.457472291647143 1104 10-11 0.4850906971449689 1104 12-13 0.4910238662414983 1104 14-15 0.5344024929548254 1104 16-17 0.52005214895145 1104 18-19 0.5759512321474887 1104 20-21 0.6216629626925325 1104 22-23 0.5312270741350034 1104 24-25 0.5361001566050589 1104 26-27 0.5433887314754671 1104 28-29 0.5007252610787845 1104 30-31 0.6023694973546867 1104 32-33 0.5911076529630961 1104 34-35 0.46751063886931377 1104 36-37 0.530368587560659 1104 38-39 0.5622171480535982 1104 40-41 0.581030957198692 1104 42-43 0.5767467764452547 1104 44-45 0.483003512438664 1104 46-47 0.5087036020845588 1104 48-49 0.4603501024301906 1104 50-51 0.4164457425718666 1104 52-53 0.4488487175852356 1104 54-55 0.5389519795598474 1104 56-57 0.5441008719411968 1104 58-59 0.5308071248539719 1104 60-61 0.5055511591692934 1104 62-63 0.4347416611519108 1104 64-65 0.4080893706004929 1104 66-67 0.4021542710855641 1104 68-69 0.4576893439663934 1104 70-71 0.5097836318824847 1104 72-73 0.4608431532511261 1104 74-75 0.4805126063840994 1104 76-77 0.48803575002586186 1104 78-79 0.5782093838134799 1104 80-81 0.5893599482949909 1104 82-83 0.505325907965176 1104 84-85 0.5674991093253077 1104 86-87 0.666402368322899 1104 88-89 0.7050583222742013 1104 90-91 0.7113082500071783 1104 92-93 0.7560552833423664 1104 94-95 0.9456383894611662 1104 96-97 0.9425891713984278 1104 98-99 1.0512247847755845 1104 100-101 1.3733156199974026 1105 1 0.28373884901801816 1105 2 0.30055074690213957 1105 3 0.333681765180053 1105 4 0.1118912111550543 1105 5 0.18640574777011665 1105 6 0.28342365682179604 1105 7 0.30213851426827176 1105 8 0.3455265767970275 1105 9 0.36409462037024554 1105 10-11 0.3897685961263164 1105 12-13 0.5466142404220946 1105 14-15 0.5409336917794647 1105 16-17 0.5068602340366724 1105 18-19 0.4409360322471372 1105 20-21 0.439510762663339 1105 22-23 0.3535050596215896 1105 24-25 0.500033607987568 1105 26-27 0.48383391265795694 1105 28-29 0.5319618448811312 1105 30-31 0.3202336440350635 1105 32-33 0.5210478344316201 1105 34-35 0.5650936114522835 1105 36-37 0.5460489314644192 1105 38-39 0.6555759063345477 1105 40-41 0.43335681461902453 1105 42-43 0.5515210501728447 1105 44-45 0.48360077549424574 1105 46-47 0.46886193928068565 1105 48-49 0.535943743279482 1105 50-51 0.46840174733446815 1105 52-53 0.36710100171376325 1105 54-55 0.4993789748608748 1105 56-57 0.48593779566956385 1105 58-59 0.7089053943749875 1105 60-61 0.5989185385685403 1105 62-63 0.39788905367129246 1105 64-65 0.35070954631625284 1105 66-67 0.4341687604453597 1105 68-69 0.3150181900410729 1105 70-71 0.21049917133459672 1105 72-73 0.37562460188770075 1105 74-75 0.6835285809821308 1105 76-77 0.6154622272578223 1105 78-79 0.7078575031800156 1105 80-81 0.5807584113033144 1105 82-83 0.6788080698689747 1105 84-85 0.6840078424513116 1105 86-87 0.6381186846112747 1105 88-89 0.6358212524813709 1105 90-91 0.7349317848848784 1105 92-93 0.7610667594542484 1105 94-95 0.9359457797778177 1105 96-97 0.9442005853847952 1105 98-99 1.035117996253085 1105 100-101 1.1769570199707786 1106 1 0.15856238653136145 1106 2 0.08971368085899911 1106 3 0.18415034937965657 1106 4 0.18085791569659193 1106 5 0.17862731265979903 1106 6 0.21662614967721794 1106 7 0.24957460432909073 1106 8 0.17266924613058166 1106 9 0.3998871599588796 1106 10-11 0.243628311970852 1106 12-13 0.32341110588490807 1106 14-15 0.337008007918854 1106 16-17 0.299147845196309 1106 18-19 0.2919073975894406 1106 20-21 0.14673484732777808 1106 22-23 0.09250569504304451 1106 24-25 0.2925759355082036 1106 26-27 0.31759529468398284 1106 28-29 0.2479562325132605 1106 30-31 0.22289169338508685 1106 32-33 0.35254650568084145 1106 34-35 0.5642206894839177 1106 36-37 0.35710377803987114 1106 38-39 0.36067970227348667 1106 40-41 0.45333737635663596 1106 42-43 0.38362618894826994 1106 44-45 0.6069668345978343 1106 46-47 0.39446637220616054 1106 48-49 0.32007064915258354 1106 50-51 0.2463716383737733 1106 52-53 0.30622887793491316 1106 54-55 0.4039558092099398 1106 56-57 0.4591714492177488 1106 58-59 0.5417527711486088 1106 60-61 0.5487249384833959 1106 62-63 0.3290283831294971 1106 64-65 0.24531135930201486 1106 66-67 0.193420324740309 1106 68-69 0.21321655109193216 1106 70-71 0.3410626305314608 1106 72-73 0.3083994373198067 1106 74-75 0.5182454428139671 1106 76-77 0.4788595253080494 1106 78-79 0.2997189437399328 1106 80-81 0.2803507868718249 1106 82-83 0.456092065699611 1106 84-85 0.4793418769393547 1106 86-87 0.5347179277398055 1106 88-89 0.4256900363848608 1106 90-91 0.45740794092936454 1106 92-93 0.4053324720496825 1106 94-95 0.4644274511553732 1106 96-97 0.45023474163587096 1106 98-99 0.5603650437344072 1106 100-101 0.7025507321154656 1107 1 0.19864808438427417 1107 2 0.1692709820532805 1107 3 0.10897747056280949 1107 4 0.04620559704724059 1107 5 0.20207934995079313 1107 6 0.07816258975442025 1107 7 0.17791673797902519 1107 8 0.02640853488246364 1107 9 0.10874656496778812 1107 10-11 0.18027999803597794 1107 12-13 0.18945077458072035 1107 14-15 0.1989329286688033 1107 16-17 0.2161162482437291 1107 18-19 0.32340501713723313 1107 20-21 0.3470931150226235 1107 22-23 0.27822011001672564 1107 24-25 0.20466915198655045 1107 26-27 0.3120747384504696 1107 28-29 0.3183605356595791 1107 30-31 0.33578693067104837 1107 32-33 0.36991011650935945 1107 34-35 0.3668577553002166 1107 36-37 0.23346070660742413 1107 38-39 0.16184821279728112 1107 40-41 0.3262351549946487 1107 42-43 0.3619153318674577 1107 44-45 0.374361130482022 1107 46-47 0.4371443671659492 1107 48-49 0.3868641473826955 1107 50-51 0.2670639876452441 1107 52-53 0.17942209858328795 1107 54-55 0.3500858335927646 1107 56-57 0.31733450016201914 1107 58-59 0.32738185806363873 1107 60-61 0.37926357136188216 1107 62-63 0.06177315349118828 1107 64-65 0.09360818135308335 1107 66-67 0.21245115692688898 1107 68-69 0.21691941117515512 1107 70-71 0.04531829369452112 1107 72-73 0.22496736105092197 1107 74-75 0.3601941956238086 1107 76-77 0.2764677825778499 1107 78-79 0.43898621608239097 1107 80-81 0.3751658385997487 1107 82-83 0.5313357561833847 1107 84-85 0.5451739100918083 1107 86-87 0.5633032207860218 1107 88-89 0.6491301537119263 1107 90-91 0.5998837685800602 1107 92-93 0.6100320850245815 1107 94-95 0.6887389453838253 1107 96-97 0.7124182823293701 1107 98-99 0.7366353460294128 1107 100-101 1.0253358808632385 1108 1 0.11030663460219614 1108 2 0.11647462266208208 1108 3 0.05218175335217268 1108 4 0.1393697435516188 1108 5 0.11215504401121734 1108 6 0.1717921189607523 1108 7 0.12462922938193799 1108 8 0.12146275355287628 1108 9 0.16081948020985948 1108 10-11 0.2227346737645206 1108 12-13 0.13205707627476926 1108 14-15 0.1671584688239136 1108 16-17 0.19933888471205563 1108 18-19 0.14550455115991667 1108 20-21 0.10857020959164032 1108 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-0.6843039628051351 2316 90-91 -0.7236924761587389 2316 92-93 -0.5528558770490299 2316 94-95 -1.0618955366118854 2316 96-97 -0.6232248207502913 2316 98-99 -0.6699976766045239 2316 100-101 -0.8353317665115583 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 15.0 4 21.0 5 37.0 6 105.0 7 287.0 8 856.0 9 1508.0 10 2961.0 11 4655.0 12 6895.0 13 9176.0 14 11400.0 15 13939.0 16 16657.0 17 19712.0 18 16774.0 19 12737.0 20 12012.0 21 12825.0 22 14099.0 23 16804.0 24 20492.0 25 25839.0 26 33325.0 27 43420.0 28 55856.0 29 69681.0 30 85683.0 31 103686.0 32 126233.0 33 154206.0 34 191326.0 35 239750.0 36 313931.0 37 434169.0 38 431758.0 39 84909.0 40 23.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.59809897157732 22.264315294328604 15.3296888453316 22.80789688876248 2 14.624777061325284 22.200421627032053 41.22314349811311 21.95165781352955 3 17.88103322082734 26.97759489649938 31.7761159077085 23.36525597496478 4 12.075517566145226 18.873584994036683 40.48584500480588 28.565052435012213 5 11.626459162012921 39.017722681252586 36.979117757422515 12.376700399311977 6 28.167099862196675 41.005338236442064 18.188724859395013 12.638837041966253 7 24.623719114709587 34.02733096294441 23.863138829281375 17.485811093064623 8 23.415060734252048 38.244706113305426 21.01965839809039 17.32057475435214 9 24.27247008323405 17.01794175123456 22.09626615729493 36.61332200823646 10-11 22.94879786131883 28.139052919452052 29.90905658671215 19.003092632516967 12-13 23.635336775431135 25.51473995106971 30.143544830032738 20.706378443466424 14-15 20.842963594424994 27.35169333513154 27.903270121722894 23.902072948720573 16-17 19.99089561138327 30.52847188865441 28.630886986173 20.849745513789323 18-19 19.831549488882867 29.292249715294638 31.008654965698174 19.86754583012432 20-21 20.592013256237145 28.476641794476542 31.182511690599178 19.74883325868714 22-23 20.196707108398165 28.31901462116212 30.981166694232375 20.50311157620734 24-25 20.156456592637646 28.513283662058857 31.105964204762714 20.224295540540776 26-27 20.159976720540993 28.69624209091587 30.877222881111294 20.266558307431843 28-29 20.090472103468677 28.71140627855994 30.971875665392336 20.22624595257905 30-31 20.432852418406615 28.43966567507954 30.988144430993554 20.139337475520293 32-33 20.33115151449561 28.586864367063498 30.824483242498836 20.25750087594206 34-35 20.359069216653552 28.629340823958454 30.934887833866426 20.076702125521564 36-37 20.41504894419577 28.499758283569893 30.894876345912248 20.190316426322088 38-39 20.22453044259196 28.784205311474498 30.87970813322431 20.111556112709238 40-41 20.493674349368828 28.68187337260088 30.78590866547282 20.038543612557476 42-43 20.38798367523614 28.678134710219982 30.510458051849675 20.423423562694204 44-45 20.43769879831665 28.935315392363943 30.41181166097956 20.215174148339848 46-47 20.5362779499838 28.788447079161113 30.09074584111352 20.584529129741565 48-49 20.518056699939248 28.947511822149117 30.01687719032462 20.517554287587014 50-51 20.614845958439307 29.13501630222044 29.682527759849187 20.567609979491067 52-53 20.63782465064258 29.211584417775033 29.578960522156887 20.571630409425502 54-55 20.69317031325156 29.101608434488146 29.76225100050071 20.442970251759583 56-57 20.683056075770423 29.219595255228192 29.604700251893064 20.492648417108324 58-59 20.574017233619337 29.12746033405525 29.79326343234996 20.505258999975457 60-61 20.627472253019423 29.271050145663065 29.698821134192833 20.40265646712468 62-63 20.56842616584074 29.24240446336676 29.696802191242544 20.49236717954995 64-65 20.41928514081895 29.12512214703743 29.705152304886752 20.750440407256864 66-67 20.467806237894322 29.17068211750809 29.606762500263926 20.754749144333655 68-69 20.56442583867371 29.196119131685933 29.599252773238682 20.640202256401675 70-71 20.594692752794582 28.9293818334507 29.635396935701163 20.84052847805355 72-73 20.699145362992848 29.069115073996226 29.546605594533077 20.685133968477846 74-75 20.54556762632163 29.248938721971314 29.40649806344612 20.798995588260937 76-77 20.47687795236255 29.203149651428557 29.524922800003406 20.795049596205487 78-79 20.438192731298173 29.232229433243607 29.479830663482026 20.849747171976194 80-81 20.50748078552789 29.16642675546408 29.407155910336456 20.918936548671574 82-83 20.631951225827095 29.18627014915972 29.521984818547914 20.659793806465277 84-85 20.672507490059946 29.170291096982222 29.29907027807415 20.85813113488368 86-87 20.421924264316324 29.228333506584647 29.477602856212105 20.87213937288693 88-89 20.580927133891862 29.134135313939467 29.37556033016972 20.909377221998948 90-91 20.530297833612387 29.107813120446437 29.56765963860369 20.794229407337486 92-93 20.54309751413342 29.184476368672136 29.413034479480014 20.859391637714428 94-95 20.27451854817455 29.136984512079096 29.435049120954798 21.153447818791555 96-97 20.324639466597212 29.040758369294252 29.551740248237568 21.082861915870968 98-99 20.19338710693367 29.138931965562538 29.430090777246605 21.23759015025719 100-101 20.249941745051615 29.204104085265925 29.30770708136719 21.238247088315273 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 191.0 1 136.5 2 111.5 3 150.5 4 129.0 5 143.0 6 273.5 7 614.0 8 1058.5 9 1334.5 10 1301.5 11 1208.0 12 1371.5 13 1581.5 14 1810.5 15 2153.0 16 2445.5 17 2810.5 18 3285.5 19 3813.5 20 4450.0 21 5278.0 22 6491.0 23 8346.5 24 10828.5 25 14297.0 26 19660.5 27 26115.0 28 33001.0 29 41246.5 30 49312.0 31 57167.5 32 66564.5 33 75435.0 34 82659.0 35 89075.5 36 97899.5 37 105365.5 38 106650.0 39 106363.5 40 106240.0 41 105335.0 42 104575.5 43 105457.5 44 105834.0 45 105725.0 46 103873.5 47 100326.5 48 97010.5 49 92056.0 50 85374.5 51 77726.0 52 69754.5 53 61941.0 54 54030.5 55 45015.5 56 36046.5 57 28040.5 58 21768.5 59 16807.5 60 12025.0 61 8174.0 62 5375.5 63 3176.5 64 1773.5 65 1031.0 66 557.0 67 303.5 68 168.5 69 84.0 70 43.5 71 24.5 72 16.0 73 12.5 74 9.0 75 5.5 76 4.5 77 2.0 78 1.0 79 1.5 80 0.5 81 1.0 82 1.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.004289419085132602 2 0.006492093750470967 3 0.0018935273438873653 4 0.17273606586074536 5 0.0023958917412452373 6 6.569380580833716E-4 7 7.728683036274961E-5 8 5.410078125392472E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 2.705039062696236E-4 24-25 6.376163504926842E-4 26-27 0.0028596127234217354 28-29 1.5457366072549921E-4 30-31 0.0062022681366106554 32-33 0.021195913226984078 34-35 0.013563838728662556 36-37 7.72868303627496E-4 38-39 2.705039062696236E-4 40-41 0.0017389536831618662 42-43 0.01093608649632907 44-45 0.007129710100963651 46-47 0.01010525306992951 48-49 0.009544923549799576 50-51 0.19267606809433474 52-53 0.11384350112433016 54-55 0.016751920481125976 56-57 0.006067016183475844 58-59 0.016867850726670103 60-61 0.1237555371183528 62-63 0.240130181937063 64-65 0.2452117910334138 66-67 0.2545634975073065 68-69 0.2506025474512156 70-71 0.23808208093245015 72-73 0.1604281381254775 74-75 0.27622313171646706 76-77 0.21288657423419377 78-79 0.005197539341894911 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 1.159302455441244E-4 90-91 0.0062022681366106554 92-93 0.01762139732270691 94-95 0.0026663956475148614 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2587763.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.80968829195541 #Duplication Level Percentage of deduplicated Percentage of total 1 74.87566610171132 44.03414584094175 2 15.038072593080349 17.687687234217055 3 4.7337595048755645 8.351727627924394 4 1.9562576075907452 4.601876004847125 5 1.0135879600679096 2.9804395994036352 6 0.5754636930317293 2.0305704246320104 7 0.3798432161954229 1.5636922800986652 8 0.2572143972933604 1.2101358823220563 9 0.18277851284224628 0.9674232630047704 >10 0.8880529665863939 9.402727470833288 >50 0.06616495369481268 2.6867032833808344 >100 0.031951174993264275 3.3746192079968704 >500 7.91545357993477E-4 0.3364439972953416 >1k 2.6384845266231487E-4 0.2844625656810195 >5k 1.3192422633115744E-4 0.48734531742133474 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6753 0.26095898271982404 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5810 0.2245182422037876 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3417 0.1320445496747577 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 3.8643415181374804E-5 0.0 3 0.0 0.0 0.0 7.728683036274961E-5 0.0 4 0.0 0.0 0.0 7.728683036274961E-5 0.0 5 0.0 0.0 0.0 1.159302455441244E-4 0.0 6 1.5457366072549921E-4 0.0 0.0 1.159302455441244E-4 0.0 7 1.5457366072549921E-4 0.0 0.0 1.159302455441244E-4 0.0 8 1.5457366072549921E-4 0.0 0.0 1.159302455441244E-4 0.0 9 1.5457366072549921E-4 0.0 0.0 1.159302455441244E-4 0.0 10-11 1.738953683161866E-4 0.0 0.0 1.159302455441244E-4 0.0 12-13 1.93217075906874E-4 0.0 0.0 1.159302455441244E-4 0.0 14-15 1.93217075906874E-4 0.0 0.0 1.159302455441244E-4 0.0 16-17 2.318604910882488E-4 0.0 0.0 1.159302455441244E-4 0.0 18-19 2.318604910882488E-4 0.0 0.0 1.159302455441244E-4 0.0 20-21 2.318604910882488E-4 0.0 0.0 1.159302455441244E-4 0.0 22-23 2.318604910882488E-4 0.0 0.0 1.159302455441244E-4 0.0 24-25 2.318604910882488E-4 0.0 0.0 1.159302455441244E-4 0.0 26-27 2.318604910882488E-4 0.0 0.0 2.125387834975614E-4 0.0 28-29 2.318604910882488E-4 0.0 0.0 5.410078125392472E-4 0.0 30-31 2.318604910882488E-4 0.0 0.0 0.0014684497768922425 0.0 32-33 2.318604910882488E-4 0.0 0.0 0.004772461774899788 0.0 34-35 2.318604910882488E-4 0.0 0.0 0.009757462333297138 0.0 36-37 2.705039062696236E-4 0.0 0.0 0.019302385883096712 0.0 38-39 2.705039062696236E-4 0.0 0.0 0.03379366657611226 0.0 40-41 2.705039062696236E-4 0.0 0.0 0.05104795145459612 0.0 42-43 2.705039062696236E-4 0.0 0.0 0.06845680999380546 0.0 44-45 2.705039062696236E-4 0.0 0.0 0.0895561146828361 0.0 46-47 2.705039062696236E-4 0.0 0.0 0.11672243555534259 0.0 48-49 2.705039062696236E-4 0.0 0.0 0.14657447378295463 0.0 50-51 2.705039062696236E-4 0.0 0.0 0.1781847874013192 0.0 52-53 2.705039062696236E-4 0.0 0.0 0.20909951954641903 3.8643415181374804E-5 54-55 2.705039062696236E-4 0.0 0.0 0.24372401954893086 3.8643415181374804E-5 56-57 2.705039062696236E-4 0.0 0.0 0.2862124545408525 3.8643415181374804E-5 58-59 2.705039062696236E-4 0.0 0.0 0.3349031576693847 3.8643415181374804E-5 60-61 2.705039062696236E-4 0.0 0.0 0.391747621401187 3.8643415181374804E-5 62-63 3.0914732145099843E-4 0.0 0.0 0.44865005025576143 3.8643415181374804E-5 64-65 3.0914732145099843E-4 0.0 0.0 0.5059195915545589 3.8643415181374804E-5 66-67 3.0914732145099843E-4 0.0 0.0 0.5746469054546339 3.8643415181374804E-5 68-69 3.0914732145099843E-4 0.0 0.0 0.6505039294556727 3.8643415181374804E-5 70-71 3.0914732145099843E-4 0.0 0.0 0.7409488426876805 3.8643415181374804E-5 72-73 3.477907366323732E-4 0.0 0.0 0.8363207913553135 7.728683036274961E-5 74-75 3.477907366323732E-4 0.0 0.0 0.9324849300341647 7.728683036274961E-5 76-77 3.477907366323732E-4 0.0 0.0 1.0417105430443205 7.728683036274961E-5 78-79 3.477907366323732E-4 0.0 0.0 1.1704317590134798 7.728683036274961E-5 80-81 3.477907366323732E-4 0.0 0.0 1.3142045851957849 7.728683036274961E-5 82-83 3.477907366323732E-4 0.0 0.0 1.4670006488229408 7.728683036274961E-5 84-85 3.477907366323732E-4 0.0 0.0 1.6221539607761608 7.728683036274961E-5 86-87 3.477907366323732E-4 0.0 0.0 1.7909870417035871 7.728683036274961E-5 88-89 3.477907366323732E-4 0.0 0.0 1.9860396798315767 7.728683036274961E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3460 0.0 57.550625 1 GTATCAA 6660 0.0 43.59926 1 ATCAACG 8070 0.0 35.5063 3 TCAACGC 8195 0.0 35.0227 4 CAACGCA 8290 0.0 34.623062 5 AACGCAG 8600 0.0 33.59503 6 TATCAAC 8610 0.0 33.448235 2 ACGCAGA 9705 0.0 29.427757 7 CGCAGAG 9780 0.0 29.153576 8 GCAGAGT 11260 0.0 25.026257 9 GTGGTAT 1800 0.0 22.969887 1 GAGTACT 8410 0.0 21.745842 12-13 AGAGTAC 10460 0.0 21.089636 10-11 CAGAGTA 11165 0.0 20.650253 10-11 TGGTATC 1920 0.0 20.04876 2 GTACTTT 9425 0.0 19.554987 14-15 AGTACTT 9370 0.0 18.783743 12-13 GTACATG 6110 0.0 18.589548 1 GTGTAGC 1695 0.0 18.504883 1 ACATGGG 5590 0.0 18.191296 3 >>END_MODULE