FastQCFastQC Report
Sat 18 Jun 2016
SRR1931004_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931004_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3819873
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1729844.52852751910862No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23100.049.4971851
GTATCAA45900.044.4588241
ATCAACG53400.037.478273
TCAACGC55100.036.3169334
CAACGCA55250.035.9389655
TATCAAC57200.034.9531562
AACGCAG62300.032.670726
ACGCAGA70650.028.5528287
CGCAGAG71800.027.4670338
GTACATG66000.026.102551
TACATGG67350.023.824542
ACATGGG64800.023.805473
GCAGAGT81950.022.7552289
CATGGGG41400.020.8192964
GAGTACA49500.019.991241
AGAGTAC73200.019.04025310-11
GTGTAGC13750.019.0175721
GAGTACT50400.019.01207712-13
GTGGTAT16150.018.4137861
CAGAGTA79750.018.1077810-11
Produced by FastQC (version 0.11.5)