##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931004_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3819873 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.970813165778026 30.0 2.0 31.0 2.0 33.0 2 20.554422882645575 30.0 2.0 31.0 2.0 33.0 3 21.313286069981906 30.0 2.0 31.0 2.0 34.0 4 22.795623571778435 32.0 2.0 35.0 2.0 37.0 5 22.10269163398888 32.0 2.0 35.0 2.0 37.0 6 23.188543179315126 32.0 2.0 35.0 2.0 37.0 7 23.440787167531486 32.0 2.0 35.0 2.0 37.0 8 23.96097488057849 32.0 2.0 35.0 2.0 37.0 9 25.439571420306383 33.0 10.0 37.0 2.0 39.0 10-11 25.91249983965435 34.0 10.0 37.5 2.0 39.0 12-13 25.883627413790983 34.0 6.0 38.0 2.0 39.0 14-15 25.913990857811243 34.0 2.0 38.5 2.0 40.0 16-17 24.6777398096743 33.0 2.0 38.0 2.0 40.0 18-19 23.50551104709502 32.0 2.0 38.0 2.0 40.0 20-21 23.149101423005426 31.5 2.0 38.0 2.0 40.0 22-23 22.77357166062851 31.0 2.0 38.0 2.0 40.0 24-25 22.634889955765544 30.0 2.0 38.0 2.0 40.0 26-27 22.36912470650202 30.0 2.0 38.0 2.0 40.0 28-29 22.217423197053932 30.0 2.0 38.0 2.0 40.0 30-31 22.28647771797649 30.0 2.0 38.0 2.0 40.0 32-33 22.210351888662267 30.0 2.0 38.0 2.0 40.0 34-35 21.923834247892536 29.5 2.0 37.5 2.0 40.0 36-37 21.442655423360932 27.5 2.0 37.0 2.0 40.0 38-39 21.38201950693125 27.0 2.0 37.0 2.0 40.0 40-41 21.313925620040248 27.5 2.0 37.0 2.0 39.0 42-43 21.21498672861637 27.5 2.0 37.0 2.0 39.0 44-45 20.857548536299504 27.0 2.0 37.0 2.0 39.0 46-47 20.558676034517376 26.0 2.0 37.0 2.0 39.0 48-49 20.383526886888646 25.0 2.0 36.5 2.0 39.0 50-51 17.82999552602927 12.0 2.0 34.0 2.0 38.5 52-53 19.899480558646843 24.5 2.0 35.5 2.0 38.5 54-55 20.800727013699145 27.5 2.0 37.0 2.0 39.0 56-57 20.827846501703068 27.5 2.0 37.0 2.0 40.0 58-59 20.568275699218272 26.5 2.0 37.0 2.0 39.5 60-61 20.293670365480736 26.0 2.0 36.5 2.0 39.0 62-63 19.85382014009366 25.5 2.0 36.0 2.0 39.0 64-65 19.526383861452985 24.5 2.0 35.5 2.0 39.0 66-67 19.193660496042668 23.0 2.0 35.0 2.0 39.0 68-69 18.758601529422577 22.0 2.0 34.5 2.0 38.0 70-71 18.42847811956052 22.0 2.0 34.0 2.0 38.0 72-73 18.036973611426347 20.0 2.0 34.0 2.0 37.5 74-75 17.594076949678694 18.0 2.0 34.0 2.0 36.5 76-77 17.200430878199356 15.5 2.0 33.0 2.0 36.0 78-79 16.839772290858885 12.0 2.0 32.5 2.0 36.0 80-81 16.423077023764925 7.5 2.0 32.0 2.0 35.0 82-83 15.974693530387004 7.0 2.0 32.0 2.0 35.0 84-85 15.595081433335611 2.0 2.0 31.5 2.0 35.0 86-87 15.117115542846582 2.0 2.0 31.0 2.0 34.0 88-89 14.71089208986791 2.0 2.0 31.0 2.0 34.0 90-91 14.237940894893626 2.0 2.0 31.0 2.0 34.0 92-93 13.684249057494842 2.0 2.0 30.0 2.0 34.0 94-95 13.11192780492964 2.0 2.0 30.0 2.0 34.0 96-97 12.44935342091216 2.0 2.0 29.0 2.0 34.0 98-99 11.72734106605115 2.0 2.0 29.0 2.0 34.0 100-101 10.639700979587541 2.0 2.0 26.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.82408932799779 1101 2 9.568868750181789 1101 3 8.944003673730109 1101 4 11.039198543596537 1101 5 11.71641706399135 1101 6 10.945762734769662 1101 7 10.602626924244245 1101 8 10.030253493011386 1101 9 9.864613849295079 1101 10-11 9.06547981852135 1101 12-13 9.009773522654314 1101 14-15 9.71271077752401 1101 16-17 10.829507028503254 1101 18-19 11.849791016743733 1101 20-21 11.916174106992077 1101 22-23 12.283410048916963 1101 24-25 12.169726150405435 1101 26-27 12.258600388236957 1101 28-29 12.183457810059778 1101 30-31 11.628828908281491 1101 32-33 11.557756044553724 1101 34-35 11.632119299543163 1101 36-37 12.100079600319106 1101 38-39 11.828099266358418 1101 40-41 11.568701217054016 1101 42-43 11.342232845284453 1101 44-45 11.53009632850792 1101 46-47 11.361663995115524 1101 48-49 11.327767582301536 1101 50-51 13.357819998522775 1101 52-53 12.191033737984842 1101 54-55 11.760866763388101 1101 56-57 11.827445647427925 1101 58-59 11.628308883611822 1101 60-61 11.439826345357819 1101 62-63 11.632708107312517 1101 64-65 11.384518159909359 1101 66-67 11.160643033483087 1101 68-69 11.06703363836608 1101 70-71 10.920402356443017 1101 72-73 10.800174150979206 1101 74-75 10.460488126440893 1101 76-77 10.500462163210479 1101 78-79 10.15051060677424 1101 80-81 9.940453831783021 1101 82-83 9.67251841914126 1101 84-85 9.561496499412804 1101 86-87 9.397053335984472 1101 88-89 8.967757936053006 1101 90-91 8.679095209552356 1101 92-93 8.524544465016696 1101 94-95 8.284503117011406 1101 96-97 7.916126560568067 1101 98-99 7.427694120171715 1101 100-101 6.860695952338196 1102 1 8.003776555530646 1102 2 9.400965974385588 1102 3 8.72376976811669 1102 4 10.679761866942709 1102 5 11.497939630215726 1102 6 10.680086461406841 1102 7 10.223524262587016 1102 8 9.645368186817763 1102 9 9.36492812776245 1102 10-11 8.60926439707907 1102 12-13 8.73189329722986 1102 14-15 9.373191548279067 1102 16-17 10.549346005374264 1102 18-19 11.583910244403402 1102 20-21 11.869074544524775 1102 22-23 12.026173717850554 1102 24-25 11.840313569755164 1102 26-27 11.856815579816036 1102 28-29 11.633518644542434 1102 30-31 11.520325951765265 1102 32-33 11.604040385321941 1102 34-35 11.272968564773578 1102 36-37 11.614556027452473 1102 38-39 11.41905454607814 1102 40-41 10.921540225630284 1102 42-43 10.833631206122114 1102 44-45 10.84942128372423 1102 46-47 10.872119797972164 1102 48-49 10.479875388138478 1102 50-51 12.606913029704902 1102 52-53 11.703455663762814 1102 54-55 11.139064437806702 1102 56-57 10.982993871140295 1102 58-59 11.194310334127827 1102 60-61 11.071889090082855 1102 62-63 10.852250045455541 1102 64-65 10.660762789829619 1102 66-67 10.635605185319612 1102 68-69 10.543977765757845 1102 70-71 10.357696074044195 1102 72-73 10.34954480141278 1102 74-75 9.863494380304974 1102 76-77 9.698791691911673 1102 78-79 9.374492075045197 1102 80-81 9.063792475748716 1102 82-83 8.976093188089122 1102 84-85 8.783442885804902 1102 86-87 8.60536328668278 1102 88-89 8.46403494497804 1102 90-91 8.258917977105183 1102 92-93 8.025898478110975 1102 94-95 7.657776170704276 1102 96-97 7.294995395875052 1102 98-99 7.085982669814236 1102 100-101 6.316565786361119 1103 1 8.183587078102747 1103 2 9.67800846836839 1103 3 8.905780292560635 1103 4 11.068266832789352 1103 5 11.751625408411549 1103 6 10.80185519037784 1103 7 10.419923029353665 1103 8 9.842735827935314 1103 9 9.581584148315297 1103 10-11 9.12063565553552 1103 12-13 8.842381922656685 1103 14-15 9.73335689061598 1103 16-17 10.795567317412246 1103 18-19 11.70697678534454 1103 20-21 12.066153707155276 1103 22-23 12.338339418177661 1103 24-25 12.310205930915021 1103 26-27 12.3249814571884 1103 28-29 11.94272731851241 1103 30-31 11.753683892050319 1103 32-33 11.861556327194943 1103 34-35 11.71780541405424 1103 36-37 12.102481755014914 1103 38-39 11.771520466814902 1103 40-41 11.342146246885083 1103 42-43 11.093892032010967 1103 44-45 11.268539968784882 1103 46-47 11.104507791495589 1103 48-49 11.037294306087748 1103 50-51 12.81769233693537 1103 52-53 11.73295895040923 1103 54-55 11.480677074470826 1103 56-57 11.139931530149557 1103 58-59 11.020070010392374 1103 60-61 10.937650954065298 1103 62-63 10.98740480017376 1103 64-65 10.694333437908874 1103 66-67 10.482745221882968 1103 68-69 10.448601942341387 1103 70-71 10.127760488882306 1103 72-73 10.115889120911621 1103 74-75 10.00595995397062 1103 76-77 9.595821348163085 1103 78-79 9.232718401285801 1103 80-81 8.960095622474782 1103 82-83 8.830000428850402 1103 84-85 8.599668733165366 1103 86-87 8.396954884598165 1103 88-89 8.130141228291018 1103 90-91 8.091986132511929 1103 92-93 7.872293091723336 1103 94-95 7.584939496129069 1103 96-97 7.3231192698437795 1103 98-99 6.759269815392237 1103 100-101 6.150562259494697 1104 1 7.866252532287742 1104 2 9.513681927050182 1104 3 8.767442946326312 1104 4 10.90050186491106 1104 5 11.728487216501883 1104 6 10.789825481515056 1104 7 10.477249083714536 1104 8 9.919628968674914 1104 9 9.68821440991358 1104 10-11 9.00750961013048 1104 12-13 8.846482819145521 1104 14-15 9.53271107572533 1104 16-17 10.47060306099942 1104 18-19 11.518849582187901 1104 20-21 11.95055393250541 1104 22-23 12.003873401918618 1104 24-25 11.873231312529715 1104 26-27 11.858212532032226 1104 28-29 11.74431369973047 1104 30-31 11.539649078619277 1104 32-33 11.536822441024054 1104 34-35 11.506646034588684 1104 36-37 11.73620656147903 1104 38-39 11.261215042375508 1104 40-41 10.929472962089289 1104 42-43 10.727567498482795 1104 44-45 10.756132156749143 1104 46-47 10.756048864120867 1104 48-49 10.54606917015878 1104 50-51 12.653100054947654 1104 52-53 11.501357792070788 1104 54-55 11.0680973028343 1104 56-57 10.790456300994475 1104 58-59 10.852862262297691 1104 60-61 10.698165156519885 1104 62-63 10.601368509158256 1104 64-65 10.633577982985212 1104 66-67 10.305387883351681 1104 68-69 10.10978702197875 1104 70-71 9.772093935885795 1104 72-73 9.720287441553065 1104 74-75 9.571907940752222 1104 76-77 9.364171774757871 1104 78-79 9.044687836898799 1104 80-81 8.876426700648338 1104 82-83 8.676501416479487 1104 84-85 8.321176179326091 1104 86-87 8.063937365488249 1104 88-89 7.628932382533 1104 90-91 7.4106194332240936 1104 92-93 7.082225158814499 1104 94-95 6.985751089566508 1104 96-97 6.367896126140593 1104 98-99 6.090006723326685 1104 100-101 5.450522749228085 1105 1 8.003980069153553 1105 2 9.219715870165391 1105 3 8.724551040618778 1105 4 10.885153916798618 1105 5 11.067941305318548 1105 6 10.671764194363622 1105 7 10.193471515797373 1105 8 9.952407332682078 1105 9 9.469445258531135 1105 10-11 8.84266628835353 1105 12-13 8.656311009509352 1105 14-15 9.541830155836585 1105 16-17 10.487871153139555 1105 18-19 11.153183136085648 1105 20-21 11.581350148964031 1105 22-23 11.972276526717536 1105 24-25 11.906249520190311 1105 26-27 12.00330284751869 1105 28-29 11.774762499340575 1105 30-31 11.358596101129862 1105 32-33 11.471242156383138 1105 34-35 11.521281239768769 1105 36-37 11.562049228505735 1105 38-39 11.456531371295942 1105 40-41 11.015258912198089 1105 42-43 10.896297105728124 1105 44-45 10.65995891214246 1105 46-47 10.574904542712272 1105 48-49 10.556732573116825 1105 50-51 12.089396551544151 1105 52-53 11.011907751274428 1105 54-55 10.802565179096547 1105 56-57 10.761934588417805 1105 58-59 10.685452848995311 1105 60-61 10.516340482540496 1105 62-63 10.658309710950395 1105 64-65 10.373424766966362 1105 66-67 10.187957169899573 1105 68-69 9.982606375756014 1105 70-71 9.896220217505764 1105 72-73 9.663408664821407 1105 74-75 9.408263682264614 1105 76-77 9.106052580458972 1105 78-79 8.840187523161728 1105 80-81 8.596963869843563 1105 82-83 8.431272439249943 1105 84-85 8.309007910870807 1105 86-87 8.100786314571346 1105 88-89 7.792225864253242 1105 90-91 7.408590308802831 1105 92-93 7.138879899628996 1105 94-95 7.0047351579719805 1105 96-97 6.653568018782877 1105 98-99 6.331234018613088 1105 100-101 5.533758973467831 1106 1 7.9239507870500425 1106 2 9.327542366774164 1106 3 8.914263507913116 1106 4 10.724669284129678 1106 5 11.34007896815406 1106 6 10.515646474765159 1106 7 9.818343060197403 1106 8 9.422170327770168 1106 9 9.026543051057732 1106 10-11 8.596672948415556 1106 12-13 8.47915832458525 1106 14-15 9.258473267498857 1106 16-17 10.411253305374156 1106 18-19 11.759509155821046 1106 20-21 11.700151083774397 1106 22-23 11.878522413251694 1106 24-25 11.70327523902208 1106 26-27 11.893831541901747 1106 28-29 11.768015260702981 1106 30-31 11.46962330715995 1106 32-33 11.619028447832445 1106 34-35 11.59055777782973 1106 36-37 11.787474661906273 1106 38-39 11.271240663283319 1106 40-41 11.234612581351886 1106 42-43 10.943143275384198 1106 44-45 10.538072156290081 1106 46-47 10.539614758425305 1106 48-49 10.519244899081134 1106 50-51 12.512257376107865 1106 52-53 11.649619082386202 1106 54-55 10.951441466827852 1106 56-57 10.902135360190595 1106 58-59 10.744948286945196 1106 60-61 10.60225562910112 1106 62-63 10.687255809248661 1106 64-65 10.6166353833454 1106 66-67 10.364916449763047 1106 68-69 10.078451573190826 1106 70-71 9.786298595628246 1106 72-73 9.594520091538786 1106 74-75 9.242734930148703 1106 76-77 9.233501319457979 1106 78-79 8.97818867320018 1106 80-81 8.72931355081181 1106 82-83 8.563294197607929 1106 84-85 8.373724708129188 1106 86-87 7.932814885406094 1106 88-89 7.474734426035477 1106 90-91 7.315325192609686 1106 92-93 7.188395636450316 1106 94-95 7.064542353333026 1106 96-97 6.643490749131189 1106 98-99 6.343471074329912 1106 100-101 5.735231045777075 1107 1 7.5943777704239785 1107 2 8.780234436771089 1107 3 8.570722249330615 1107 4 10.37835729778212 1107 5 11.275335232882682 1107 6 10.104924011775307 1107 7 10.032482588357514 1107 8 8.611268889858621 1107 9 6.095527951094695 1107 10-11 8.00320324099432 1107 12-13 7.941687769707201 1107 14-15 8.65716076452766 1107 16-17 9.67597125797285 1107 18-19 11.07665446337462 1107 20-21 11.444884372576222 1107 22-23 10.869682085367891 1107 24-25 11.074821864669307 1107 26-27 11.216995454212523 1107 28-29 11.158918415842393 1107 30-31 10.927051275756995 1107 32-33 11.129501415561169 1107 34-35 11.366554073177397 1107 36-37 11.56103991559516 1107 38-39 11.250994049472336 1107 40-41 11.106352395134525 1107 42-43 10.87075714845098 1107 44-45 10.340454033811959 1107 46-47 10.53963652695657 1107 48-49 10.84824580959998 1107 50-51 12.63865650449345 1107 52-53 11.25508106761804 1107 54-55 11.052402702106505 1107 56-57 10.665151486609613 1107 58-59 10.76902203897167 1107 60-61 10.70974439193061 1107 62-63 10.717601384379321 1107 64-65 10.807233669901255 1107 66-67 10.899886837284132 1107 68-69 10.593025756464638 1107 70-71 10.311304149938731 1107 72-73 9.94439708417799 1107 74-75 10.047902694579967 1107 76-77 9.983126949229472 1107 78-79 9.507606993790127 1107 80-81 9.223685870068728 1107 82-83 9.238844122855157 1107 84-85 9.134681980201147 1107 86-87 8.926486427738672 1107 88-89 8.547393235657 1107 90-91 8.331823677665438 1107 92-93 8.022878639290049 1107 94-95 7.79512476280747 1107 96-97 7.312406547724617 1107 98-99 6.979802777266954 1107 100-101 6.373225767484291 1108 1 6.666593696937827 1108 2 8.177629948164789 1108 3 8.069850563767151 1108 4 9.311272937869454 1108 5 11.063060995695217 1108 6 9.516398895526375 1108 7 8.902585357534111 1108 8 6.055770003739525 1108 9 7.125816394495711 1108 10-11 6.091188828739405 1108 12-13 5.054262955458817 1108 14-15 7.231525796792965 1108 16-17 8.551926375779178 1108 18-19 8.461247841224495 1108 20-21 8.697265879444757 1108 22-23 9.419872988012862 1108 24-25 9.092781924566406 1108 26-27 7.7971656777528935 1108 28-29 9.57787209329382 1108 30-31 10.069723010751034 1108 32-33 10.431908774333355 1108 34-35 10.056980247492262 1108 36-37 9.799000703408346 1108 38-39 9.413919505544417 1108 40-41 9.84340806402896 1108 42-43 9.03382933644583 1108 44-45 8.727809018900562 1108 46-47 9.52740143037347 1108 48-49 10.035867451391745 1108 50-51 11.653010235921915 1108 52-53 10.712786180345883 1108 54-55 10.0132368262324 1108 56-57 10.085374180566603 1108 58-59 10.02994785392331 1108 60-61 9.983871787403306 1108 62-63 10.014941443560591 1108 64-65 9.867768296458536 1108 66-67 9.263148948501975 1108 68-69 9.610738119333469 1108 70-71 9.529332672641491 1108 72-73 9.43502492268799 1108 74-75 9.160215915180622 1108 76-77 8.679190906210358 1108 78-79 8.614141382867807 1108 80-81 8.501724303070944 1108 82-83 8.4932263711824 1108 84-85 8.31547771582202 1108 86-87 7.940147369260648 1108 88-89 7.795174603036495 1108 90-91 7.635653435262123 1108 92-93 7.399872911727034 1108 94-95 7.180091469715078 1108 96-97 6.927564565974272 1108 98-99 6.690735992295785 1108 100-101 6.161727389697289 1109 1 6.803284035099477 1109 2 8.426994087376894 1109 3 8.181460015837612 1109 4 9.456576514462878 1109 5 10.52907572316164 1109 6 9.287583446357818 1109 7 7.9048644799701755 1109 8 8.539178160075586 1109 9 7.770565520363032 1109 10-11 6.115861736345121 1109 12-13 6.686494992399048 1109 14-15 8.058760980550957 1109 16-17 7.052945992639895 1109 18-19 6.316031752751908 1109 20-21 8.592495201917828 1109 22-23 9.928811417574153 1109 24-25 9.36617388871364 1109 26-27 9.56751085761254 1109 28-29 10.196389064851502 1109 30-31 7.696973554333113 1109 32-33 6.854342920210367 1109 34-35 8.51825525660935 1109 36-37 7.238321074607089 1109 38-39 7.436380778128299 1109 40-41 8.158531412698164 1109 42-43 6.9583287648687495 1109 44-45 7.5544978959327445 1109 46-47 7.199100460458965 1109 48-49 7.7939359100977725 1109 50-51 9.759178717323355 1109 52-53 10.039378287492184 1109 54-55 10.139476525718791 1109 56-57 9.194833556676297 1109 58-59 8.998705283077989 1109 60-61 9.198891569539324 1109 62-63 9.60253375969631 1109 64-65 9.671142517463949 1109 66-67 9.25693459721651 1109 68-69 8.767651237820889 1109 70-71 8.340443813693778 1109 72-73 7.674765967431487 1109 74-75 6.94855780869662 1109 76-77 7.029912428707256 1109 78-79 7.95417644997497 1109 80-81 7.821852020157955 1109 82-83 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-18.319365534992826 2313 60-61 -18.04518657453882 2313 62-63 -17.620909463746912 2313 64-65 -17.290029643708213 2313 66-67 -16.969072728790817 2313 68-69 -16.532837162667512 2313 70-71 -16.20595423270427 2313 72-73 -15.815588122922602 2313 74-75 -15.38331032317151 2313 76-77 -15.014654115859287 2313 78-79 -14.65388215808364 2313 80-81 -14.238099139793206 2313 82-83 -13.789755482667525 2313 84-85 -13.420544844256447 2313 86-87 -12.950362684687365 2313 88-89 -12.545678756453453 2313 90-91 -12.0764603460032 2313 92-93 -11.52791031534506 2313 94-95 -10.959682929500223 2313 96-97 -10.318757160362168 2313 98-99 -9.602667636934228 2313 100-101 -8.535428078669556 2314 1 -19.70123366941823 2314 2 -18.28436122397842 2314 3 -19.045402011024414 2314 4 -20.51118905330269 2314 5 -19.79986200577609 2314 6 -20.895322536548168 2314 7 -21.136649561543866 2314 8 -21.67620645530903 2314 9 -23.153732403934892 2314 10-11 -23.642013721530336 2314 12-13 -23.622784616880566 2314 14-15 -23.64917552738555 2314 16-17 -22.40834826865437 2314 18-19 -21.22462758790743 2314 20-21 -20.86367085424823 2314 22-23 -20.485840597077868 2314 24-25 -20.346036123496372 2314 26-27 -20.08145129134961 2314 28-29 -19.93230446384203 2314 30-31 -20.013160755801195 2314 32-33 -19.928807262939817 2314 34-35 -19.653539615185522 2314 36-37 -19.17059223430622 2314 38-39 -19.116732774985255 2314 40-41 -19.04305588888914 2314 42-43 -18.947043640503654 2314 44-45 -18.60801736658252 2314 46-47 -18.319214557856053 2314 48-49 -18.123524407362545 2314 50-51 -15.655229097084462 2314 52-53 -17.67576212764823 2314 54-55 -18.548435562193298 2314 56-57 -18.577205515362774 2314 58-59 -18.319365534992826 2314 60-61 -18.04518657453882 2314 62-63 -17.620909463746912 2314 64-65 -17.290029643708213 2314 66-67 -16.969072728790817 2314 68-69 -16.532837162667512 2314 70-71 -16.20595423270427 2314 72-73 -15.815588122922602 2314 74-75 -15.38331032317151 2314 76-77 -15.014654115859287 2314 78-79 -14.65388215808364 2314 80-81 -14.238099139793206 2314 82-83 -13.789755482667525 2314 84-85 -13.420544844256447 2314 86-87 -12.950362684687365 2314 88-89 -12.545678756453453 2314 90-91 -12.0764603460032 2314 92-93 -11.52791031534506 2314 94-95 -10.959682929500223 2314 96-97 -10.318757160362168 2314 98-99 -9.602667636934228 2314 100-101 -8.535428078669556 2315 1 -19.70123366941823 2315 2 -18.28436122397842 2315 3 -19.045402011024414 2315 4 -20.51118905330269 2315 5 -19.79986200577609 2315 6 -20.895322536548168 2315 7 -21.136649561543866 2315 8 -21.67620645530903 2315 9 -23.153732403934892 2315 10-11 -23.642013721530336 2315 12-13 -23.622784616880566 2315 14-15 -23.64917552738555 2315 16-17 -22.40834826865437 2315 18-19 -21.22462758790743 2315 20-21 -20.86367085424823 2315 22-23 -20.485840597077868 2315 24-25 -20.346036123496372 2315 26-27 -20.08145129134961 2315 28-29 -19.93230446384203 2315 30-31 -20.013160755801195 2315 32-33 -19.928807262939817 2315 34-35 -19.653539615185522 2315 36-37 -19.17059223430622 2315 38-39 -19.116732774985255 2315 40-41 -19.04305588888914 2315 42-43 -18.947043640503654 2315 44-45 -18.60801736658252 2315 46-47 -18.319214557856053 2315 48-49 -18.123524407362545 2315 50-51 -15.655229097084462 2315 52-53 -17.67576212764823 2315 54-55 -18.548435562193298 2315 56-57 -18.577205515362774 2315 58-59 -18.319365534992826 2315 60-61 -18.04518657453882 2315 62-63 -17.620909463746912 2315 64-65 -17.290029643708213 2315 66-67 -16.969072728790817 2315 68-69 -16.532837162667512 2315 70-71 -16.20595423270427 2315 72-73 -15.815588122922602 2315 74-75 -15.38331032317151 2315 76-77 -15.014654115859287 2315 78-79 -14.65388215808364 2315 80-81 -14.238099139793206 2315 82-83 -13.789755482667525 2315 84-85 -13.420544844256447 2315 86-87 -12.950362684687365 2315 88-89 -12.545678756453453 2315 90-91 -12.0764603460032 2315 92-93 -11.52791031534506 2315 94-95 -10.959682929500223 2315 96-97 -10.318757160362168 2315 98-99 -9.602667636934228 2315 100-101 -8.535428078669556 2316 1 -19.70123366941823 2316 2 -18.28436122397842 2316 3 -19.045402011024414 2316 4 -20.51118905330269 2316 5 -19.79986200577609 2316 6 -20.895322536548168 2316 7 -21.136649561543866 2316 8 -21.67620645530903 2316 9 -23.153732403934892 2316 10-11 -23.642013721530336 2316 12-13 -23.622784616880566 2316 14-15 -23.64917552738555 2316 16-17 -22.40834826865437 2316 18-19 -21.22462758790743 2316 20-21 -20.86367085424823 2316 22-23 -20.485840597077868 2316 24-25 -20.346036123496372 2316 26-27 -20.08145129134961 2316 28-29 -19.93230446384203 2316 30-31 -20.013160755801195 2316 32-33 -19.928807262939817 2316 34-35 -19.653539615185522 2316 36-37 -19.17059223430622 2316 38-39 -19.116732774985255 2316 40-41 -19.04305588888914 2316 42-43 -18.947043640503654 2316 44-45 -18.60801736658252 2316 46-47 -18.319214557856053 2316 48-49 -18.123524407362545 2316 50-51 -15.655229097084462 2316 52-53 -17.67576212764823 2316 54-55 -18.548435562193298 2316 56-57 -18.577205515362774 2316 58-59 -18.319365534992826 2316 60-61 -18.04518657453882 2316 62-63 -17.620909463746912 2316 64-65 -17.290029643708213 2316 66-67 -16.969072728790817 2316 68-69 -16.532837162667512 2316 70-71 -16.20595423270427 2316 72-73 -15.815588122922602 2316 74-75 -15.38331032317151 2316 76-77 -15.014654115859287 2316 78-79 -14.65388215808364 2316 80-81 -14.238099139793206 2316 82-83 -13.789755482667525 2316 84-85 -13.420544844256447 2316 86-87 -12.950362684687365 2316 88-89 -12.545678756453453 2316 90-91 -12.0764603460032 2316 92-93 -11.52791031534506 2316 94-95 -10.959682929500223 2316 96-97 -10.318757160362168 2316 98-99 -9.602667636934228 2316 100-101 -8.535428078669556 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 920912.0 3 32094.0 4 151332.0 5 72213.0 6 34359.0 7 36219.0 8 35120.0 9 31115.0 10 32603.0 11 35761.0 12 37780.0 13 39485.0 14 41505.0 15 43155.0 16 45725.0 17 47035.0 18 46836.0 19 48080.0 20 51430.0 21 56004.0 22 60590.0 23 66019.0 24 72289.0 25 78521.0 26 86351.0 27 94690.0 28 104322.0 29 115280.0 30 127663.0 31 142416.0 32 159385.0 33 177460.0 34 195467.0 35 203969.0 36 180851.0 37 98389.0 38 17265.0 39 183.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.35510265264204 20.050958329901 13.869787861559715 23.724151155897243 2 14.760375673000315 20.520831205299686 41.8393639485409 22.879429173159103 3 18.898871160235473 25.657545921594664 29.781499255878764 25.662083662291103 4 12.152106757410017 16.97489411600963 39.663225460238564 31.209773666341782 5 12.82989251007657 38.05041188947746 35.74948920560804 13.370206394837927 6 30.06407905724765 38.332373912061854 18.254012215618072 13.349534815072426 7 26.52392714664849 32.78272941896967 22.96679821761654 17.7265452167653 8 24.43738314199005 36.685005121486384 20.87797472043511 17.999637016088457 9 25.23534428369282 17.278272770621015 21.40840989750361 36.07797304818256 10-11 23.89521497269503 27.22662596011246 29.189017300492466 19.689141766700043 12-13 24.522887586215145 24.647376345799763 29.342252283446022 21.48748378453907 14-15 21.64385585471333 26.48158601522304 26.941621034692286 24.932937095371344 16-17 21.30785447177867 29.602699038112025 27.551145554319266 21.53830093579004 18-19 21.125543931284174 28.632848858666982 29.420826829562497 20.820780380486347 20-21 21.87595397739647 27.95913014869768 29.34342259518922 20.821493278716634 22-23 21.41682244437478 27.726865512925635 29.079906177680392 21.776405865019193 24-25 21.35069354625877 27.965020595817442 29.290485416493294 21.393800441430493 26-27 21.28135729470934 28.205359644451388 29.02456416208652 21.488718898752754 28-29 21.242743541575447 28.272415570841396 29.197905337646013 21.286935549937148 30-31 21.62111960846069 27.976692914983943 29.09444211483128 21.30774536172409 32-33 21.366647116995313 28.174578922595096 28.94868515057714 21.510088809832446 34-35 21.557680479056085 28.118184103905687 29.05405910945193 21.2700763075863 36-37 21.626417944050463 27.888936945742444 28.972046983892657 21.51259812631444 38-39 21.50299763687156 28.179693659656106 28.985474869670135 21.3318338338022 40-41 21.68274649459054 28.124319981252405 28.949967359100114 21.242966165056938 42-43 21.58750563554482 28.08136421459026 28.741596070958124 21.589534078906798 44-45 21.57010205606168 28.3563743181039 28.671458239377813 21.40206538645661 46-47 21.611387625698683 28.249025247543873 28.46233650081413 21.677250625943312 48-49 21.575590972635315 28.386951965895424 28.428947471048048 21.60850959042121 50-51 21.8579406625992 28.555703923244614 28.088777395744756 21.49757801841143 52-53 21.45359042875902 28.579811573608442 28.26181844410709 21.704779553525444 54-55 21.274995551784077 28.53556177545406 28.475391100830787 21.714051571931073 56-57 21.343142064944015 28.722743779383787 28.334573385638436 21.59954077003377 58-59 21.263183099283708 28.613339843957625 28.47293272430416 21.650544332454505 60-61 21.32901439264074 28.686974737174893 28.475211827835423 21.508799042348947 62-63 21.29806273324168 28.590720860644947 28.322581492456433 21.788634913656946 64-65 21.084406171520314 28.560926677261705 28.431765728481313 21.922901422736665 66-67 21.101729335069393 28.615114414776016 28.221532429787498 22.06162382036709 68-69 21.267211049369227 28.483975538044092 28.274040841840897 21.974772570745778 70-71 21.207835178176687 28.298009141854656 28.34717220051885 22.14698347944981 72-73 21.244660458261748 28.500990263815485 28.21346867051585 22.040880607406915 74-75 21.13037423408982 28.643199090013855 28.0460206808203 22.18040599507603 76-77 21.133783988296422 28.545948309203112 28.152550068102713 22.167717634397754 78-79 21.073805969838965 28.568560843876945 28.121209601303903 22.236423584980187 80-81 21.130776337151673 28.632755611144873 28.0065837612913 22.22988429041216 82-83 21.252927001353154 28.511941483832043 28.178697906192802 22.056433608622 84-85 21.329620463060138 28.493395564053976 28.016948777628397 22.16003519525749 86-87 21.04489917595556 28.51958413110384 28.17490382471986 22.260612868220733 88-89 21.233166691559465 28.475982786589242 28.030283693911134 22.26056682794016 90-91 21.141197381482797 28.499851421815553 28.09834790078819 22.260603295913455 92-93 21.176267420989582 28.326741581215504 28.024196275828462 22.47279472196646 94-95 20.89494643598963 28.412072170108754 28.14685055101623 22.546130842885393 96-97 20.95491752189093 28.271792229569737 28.162349779048306 22.610940469491027 98-99 20.798782318022166 28.25055790499767 27.867124428242285 23.083535348737875 100-101 21.022640121521395 28.291657939051284 27.859224525946168 22.826477413481154 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 233254.0 1 210995.0 2 139401.5 3 70321.0 4 43251.0 5 33293.5 6 30872.5 7 31904.0 8 33242.0 9 33100.0 10 30529.0 11 26082.0 12 20682.5 13 15365.0 14 11139.0 15 8536.0 16 7627.0 17 8007.0 18 9256.0 19 11000.5 20 12797.0 21 14787.0 22 16866.5 23 19016.5 24 21370.0 25 23582.5 26 26334.5 27 29932.0 28 33941.0 29 39571.5 30 46688.0 31 54198.5 32 63071.5 33 72025.5 34 80145.0 35 87985.5 36 96890.0 37 105440.0 38 110655.0 39 114039.5 40 116195.5 41 116833.5 42 116647.0 43 117502.5 44 118659.5 45 119644.0 46 118909.5 47 115745.5 48 112247.5 49 107741.0 50 101772.5 51 94508.5 52 86653.5 53 78296.5 54 69338.5 55 59850.0 56 49933.0 57 40270.0 58 32076.5 59 25170.5 60 18977.5 61 13886.0 62 9990.0 63 6815.0 64 4298.0 65 2708.5 66 1724.5 67 1076.0 68 658.5 69 392.0 70 247.5 71 159.0 72 105.5 73 77.5 74 59.5 75 38.5 76 23.0 77 15.0 78 16.5 79 10.5 80 6.5 81 6.5 82 4.5 83 4.0 84 4.0 85 2.0 86 1.0 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 22.45742201376852 2 29.520300805812134 3 23.27038097863463 4 29.65370838245146 5 32.82234775868203 6 25.326993855554885 7 24.25326705887866 8 20.23381405612176 9 11.992440586375515 10-11 15.65798129937828 12-13 15.736347779101557 14-15 19.90725869682055 16-17 23.26272365599589 18-19 27.295095936435583 20-21 29.380178346243447 22-23 28.97668325622344 24-25 27.488204450776244 26-27 28.94160355592974 28-29 25.86245668376933 30-31 27.768253028307488 32-33 26.751465297406483 34-35 24.77321890020951 36-37 27.790609792524517 38-39 27.931674691802584 40-41 24.93027909566627 42-43 25.791093054664383 44-45 24.479504946892213 46-47 27.81858192667662 48-49 23.138059825549174 50-51 28.74523577092746 52-53 22.330192653001816 54-55 23.49169723705474 56-57 25.61852187232403 58-59 24.888693943489745 60-61 24.398023180351807 62-63 24.252507871334988 64-65 22.77732793734242 66-67 23.26562951176649 68-69 24.28209000665729 70-71 22.762196544230658 72-73 22.62104001886974 74-75 19.862636794469346 76-77 22.158406837085945 78-79 21.296794422222938 80-81 22.32733915499285 82-83 23.194043362174607 84-85 20.11390169254318 86-87 21.667736073948006 88-89 20.511663607664442 90-91 21.63683190514449 92-93 21.626543604983723 94-95 20.660372740140836 96-97 19.568242190250828 98-99 18.974766962147694 100-101 20.63702117845279 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3819873.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.45457582762576 #Duplication Level Percentage of deduplicated Percentage of total 1 84.47455157357675 58.6714414777167 2 9.782239750170682 13.588426249844893 3 2.683947647754794 5.592373362550896 4 1.1406441395646592 3.1689181953492227 5 0.5459022971635913 1.8957706246411872 6 0.33785309116143836 1.407926588320193 7 0.212103271668282 1.031207993576054 8 0.14964566565923362 0.8314860986243819 9 0.11324396326042249 0.7078780289962694 >10 0.5298204406990332 6.394660596684681 >50 0.02168352256894132 1.0131265179142301 >100 0.00813543627971285 0.9587793937253218 >500 1.1460023753468419E-4 0.053806293425543446 >1k 7.640015655428124E-5 0.09463858222255661 >5k 0.0 0.0 >10k+ 3.820007827714062E-5 4.589559996407831 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 172984 4.52852751910862 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 2.6178880816194676E-5 0.0 0.0 0.0 0.0 7 2.6178880816194676E-5 0.0 0.0 0.0 0.0 8 2.6178880816194676E-5 0.0 0.0 0.0 2.6178880816194676E-5 9 2.6178880816194676E-5 0.0 0.0 0.0 2.6178880816194676E-5 10-11 3.9268321224292016E-5 0.0 0.0 2.6178880816194676E-5 5.235776163238935E-5 12-13 5.235776163238935E-5 0.0 0.0 2.6178880816194676E-5 5.235776163238935E-5 14-15 5.235776163238935E-5 0.0 0.0 2.6178880816194676E-5 5.235776163238935E-5 16-17 5.235776163238935E-5 0.0 0.0 2.6178880816194676E-5 5.235776163238935E-5 18-19 5.235776163238935E-5 0.0 0.0 2.6178880816194676E-5 5.235776163238935E-5 20-21 5.235776163238935E-5 0.0 0.0 6.544720204048669E-5 5.235776163238935E-5 22-23 5.235776163238935E-5 0.0 0.0 1.4398384448907072E-4 5.235776163238935E-5 24-25 5.235776163238935E-5 0.0 0.0 1.9634160612146007E-4 5.235776163238935E-5 26-27 5.235776163238935E-5 0.0 0.0 2.3560992734575207E-4 5.235776163238935E-5 28-29 5.235776163238935E-5 1.3089440408097338E-5 0.0 5.235776163238935E-4 5.235776163238935E-5 30-31 5.235776163238935E-5 2.6178880816194676E-5 0.0 0.001937237180398406 5.235776163238935E-5 32-33 5.235776163238935E-5 2.6178880816194676E-5 0.0 0.005929516504868094 5.235776163238935E-5 34-35 5.235776163238935E-5 2.6178880816194676E-5 0.0 0.011047487704434152 5.235776163238935E-5 36-37 5.235776163238935E-5 2.6178880816194676E-5 0.0 0.019477087327248836 5.235776163238935E-5 38-39 5.235776163238935E-5 2.6178880816194676E-5 0.0 0.0324487227716733 5.235776163238935E-5 40-41 5.235776163238935E-5 2.6178880816194676E-5 0.0 0.04607483023650263 5.235776163238935E-5 42-43 5.235776163238935E-5 2.6178880816194676E-5 0.0 0.06129784943111983 5.235776163238935E-5 44-45 5.235776163238935E-5 2.6178880816194676E-5 0.0 0.08002883865510713 5.235776163238935E-5 46-47 5.235776163238935E-5 2.6178880816194676E-5 0.0 0.101259910997041 5.235776163238935E-5 48-49 5.235776163238935E-5 2.6178880816194676E-5 0.0 0.12505651365896195 5.235776163238935E-5 50-51 5.235776163238935E-5 2.6178880816194676E-5 0.0 0.15098669510740279 5.235776163238935E-5 52-53 5.235776163238935E-5 2.6178880816194676E-5 0.0 0.1824537098484688 5.235776163238935E-5 54-55 5.235776163238935E-5 2.6178880816194676E-5 0.0 0.21754649958257774 5.235776163238935E-5 56-57 5.235776163238935E-5 2.6178880816194676E-5 0.0 0.2574692928272746 5.235776163238935E-5 58-59 5.235776163238935E-5 2.6178880816194676E-5 0.0 0.30349176530214483 5.235776163238935E-5 60-61 5.235776163238935E-5 2.6178880816194676E-5 0.0 0.35228919914353174 5.235776163238935E-5 62-63 7.853664244858402E-5 2.6178880816194676E-5 0.0 0.4040055781959243 7.853664244858402E-5 64-65 7.853664244858402E-5 5.235776163238935E-5 0.0 0.4594524477646246 7.853664244858402E-5 66-67 7.853664244858402E-5 5.235776163238935E-5 0.0 0.5233289169561397 7.853664244858402E-5 68-69 7.853664244858402E-5 5.235776163238935E-5 0.0 0.5967214093243414 7.853664244858402E-5 70-71 7.853664244858402E-5 5.235776163238935E-5 0.0 0.6766062641349595 7.853664244858402E-5 72-73 7.853664244858402E-5 5.235776163238935E-5 0.0 0.759645674083929 1.3089440408097338E-4 74-75 7.853664244858402E-5 5.235776163238935E-5 0.0 0.8431824827684062 1.3089440408097338E-4 76-77 7.853664244858402E-5 5.235776163238935E-5 0.0 0.939180438721392 1.3089440408097338E-4 78-79 7.853664244858402E-5 5.235776163238935E-5 0.0 1.0488830387816557 1.3089440408097338E-4 80-81 7.853664244858402E-5 5.235776163238935E-5 0.0 1.1671723117496315 1.3089440408097338E-4 82-83 7.853664244858402E-5 5.235776163238935E-5 0.0 1.2953572016661288 1.3089440408097338E-4 84-85 9.162608285668136E-5 6.544720204048669E-5 0.0 1.42784851747689 1.3089440408097338E-4 86-87 1.047155232647787E-4 7.853664244858402E-5 0.0 1.5661908131500706 1.3089440408097338E-4 88-89 1.047155232647787E-4 7.853664244858402E-5 0.0 1.7220467800892858 1.3089440408097338E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2310 0.0 49.497185 1 GTATCAA 4590 0.0 44.458824 1 ATCAACG 5340 0.0 37.47827 3 TCAACGC 5510 0.0 36.316933 4 CAACGCA 5525 0.0 35.938965 5 TATCAAC 5720 0.0 34.953156 2 AACGCAG 6230 0.0 32.67072 6 ACGCAGA 7065 0.0 28.552828 7 CGCAGAG 7180 0.0 27.467033 8 GTACATG 6600 0.0 26.10255 1 TACATGG 6735 0.0 23.82454 2 ACATGGG 6480 0.0 23.80547 3 GCAGAGT 8195 0.0 22.755228 9 CATGGGG 4140 0.0 20.819296 4 GAGTACA 4950 0.0 19.99124 1 AGAGTAC 7320 0.0 19.040253 10-11 GTGTAGC 1375 0.0 19.017572 1 GAGTACT 5040 0.0 19.012077 12-13 GTGGTAT 1615 0.0 18.413786 1 CAGAGTA 7975 0.0 18.10778 10-11 >>END_MODULE