FastQCFastQC Report
Sat 18 Jun 2016
SRR1931004_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931004_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3819873
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64560.16901085454935283No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49720.1301613954181199No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39100.055.171421
GTATCAA79350.043.054351
CAACGCA97550.034.1894265
ATCAACG97700.034.137083
TCAACGC100500.033.327824
TATCAAC104050.032.331112
AACGCAG104700.031.9448226
ACGCAGA120250.027.655387
CGCAGAG121900.027.1642918
GTACATG100400.023.6157591
GCAGAGT141550.022.823599
TACATGG101500.022.7041442
ACATGGG97900.022.2262733
AGAGTAC132700.020.16298710-11
GAGTACT94950.019.1359712-13
GAGTACA77100.019.0431691
GGCGTCG6250.018.9760888
GCGTCGG5750.018.9760889
ACGGGCG5850.018.6524435
GTGGTAT21450.018.607461
Produced by FastQC (version 0.11.5)