##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931004_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3819873 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.362473830936263 31.0 31.0 34.0 30.0 34.0 2 31.522361345521173 31.0 31.0 34.0 30.0 34.0 3 31.598229312859353 31.0 31.0 34.0 30.0 34.0 4 35.36756169642289 37.0 35.0 37.0 33.0 37.0 5 35.23888202565897 37.0 35.0 37.0 33.0 37.0 6 35.26414700174587 37.0 35.0 37.0 32.0 37.0 7 35.141928802345 37.0 35.0 37.0 32.0 37.0 8 35.1407258827715 37.0 35.0 37.0 32.0 37.0 9 36.647797191163164 39.0 35.0 39.0 32.0 39.0 10-11 36.59292704233884 38.5 35.0 39.0 32.0 39.0 12-13 36.48097214226756 38.0 35.0 39.0 32.0 39.0 14-15 37.56093186867731 39.0 36.0 41.0 32.0 41.0 16-17 37.519032046353374 39.0 36.0 41.0 32.0 41.0 18-19 37.53041344568262 39.0 36.0 41.0 32.0 41.0 20-21 37.50400693949773 39.0 36.0 41.0 32.0 41.0 22-23 37.40322976706294 39.0 36.0 41.0 32.0 41.0 24-25 37.28529168378111 39.0 36.0 40.5 31.5 41.0 26-27 37.038005321119314 39.0 36.0 40.0 31.0 41.0 28-29 36.857673409561 39.0 36.0 40.0 30.5 41.0 30-31 36.639595478697856 38.5 35.0 40.0 30.0 41.0 32-33 36.49713118734576 38.0 35.0 40.0 30.0 41.0 34-35 36.599219790815035 38.5 35.0 40.0 30.0 41.0 36-37 36.68298697365069 39.0 35.0 40.0 30.0 41.0 38-39 36.67403327283394 39.0 35.0 40.0 30.0 41.0 40-41 36.56772450288268 39.0 35.0 40.0 30.0 41.0 42-43 36.44190343500949 39.0 35.0 40.0 30.0 41.0 44-45 36.30038145247237 39.0 35.0 40.0 30.0 41.0 46-47 36.098115696516615 38.0 35.0 40.0 29.5 41.0 48-49 35.913554717656844 38.0 35.0 40.0 29.0 41.0 50-51 35.66345870661145 38.0 34.5 40.0 28.0 41.0 52-53 35.45573216701183 38.0 34.0 40.0 27.0 41.0 54-55 35.30868761867214 38.0 34.0 40.0 27.0 41.0 56-57 35.14729887616683 38.0 34.0 40.0 27.0 41.0 58-59 34.948354042137 38.0 34.0 40.0 26.5 41.0 60-61 34.6449215719999 37.5 33.0 40.0 26.0 41.0 62-63 34.38963154010618 37.0 33.0 40.0 26.0 41.0 64-65 34.08291754725877 37.0 33.0 39.5 26.0 41.0 66-67 33.763921339793235 36.0 32.0 39.0 25.0 41.0 68-69 33.41311779213602 36.0 32.0 39.0 25.0 40.0 70-71 33.0413982349675 35.5 31.5 39.0 24.0 40.0 72-73 32.615597953125665 35.0 31.0 38.0 22.5 40.0 74-75 32.096286970797195 35.0 31.0 37.0 22.0 39.0 76-77 30.698238396930996 33.5 29.5 36.0 20.5 38.5 78-79 31.43583621759153 34.0 30.5 36.0 22.0 39.0 80-81 31.405491491471054 34.0 31.0 36.0 22.0 38.5 82-83 31.133801045217997 34.0 31.0 36.0 21.5 37.0 84-85 30.80675378474625 34.0 30.5 35.0 20.0 37.0 86-87 30.476145149328264 34.0 30.0 35.0 20.0 36.0 88-89 30.137088981754104 34.0 30.0 35.0 18.5 36.0 90-91 29.8173174867332 34.0 30.0 35.0 17.5 36.0 92-93 29.514490665003784 34.0 29.0 35.0 11.5 35.0 94-95 29.144787797918937 34.0 29.0 35.0 4.5 35.0 96-97 28.73900074688347 34.0 29.0 35.0 2.0 35.0 98-99 28.259668318815834 34.0 29.0 35.0 2.0 35.0 100-101 27.139163396269982 33.0 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.34703228427693844 1101 2 0.3905985656060089 1101 3 0.31833231780235494 1101 4 0.25280228536539084 1101 5 0.2252666315992613 1101 6 0.29323136925056303 1101 7 0.3667409866302762 1101 8 0.35107620179747556 1101 9 0.5256991917734339 1101 10-11 0.5166242848885148 1101 12-13 0.4428437104631513 1101 14-15 0.5505340887265646 1101 16-17 0.6433278369844899 1101 18-19 0.4809135601847885 1101 20-21 0.4928000788059066 1101 22-23 0.5890771663282166 1101 24-25 0.4977378052579482 1101 26-27 0.44581102509554427 1101 28-29 0.5755259852331776 1101 30-31 0.5411808667175748 1101 32-33 0.4991865139531342 1101 34-35 0.5420083283234618 1101 36-37 0.5315326632608119 1101 38-39 0.5105233476631525 1101 40-41 0.532212567918684 1101 42-43 0.511528073404321 1101 44-45 0.5523213621232586 1101 46-47 0.5755425981681626 1101 48-49 0.49621670566717313 1101 50-51 0.6183998439764977 1101 52-53 0.4578784159258973 1101 54-55 0.5317604704997692 1101 56-57 0.6228304964040206 1101 58-59 0.5918543664012219 1101 60-61 0.4673317409512592 1101 62-63 0.3450825906376238 1101 64-65 0.37224030137419106 1101 66-67 0.36324635700005103 1101 68-69 0.36359594374748383 1101 70-71 0.35901745685892905 1101 72-73 0.4308018926555164 1101 74-75 0.35783845554369975 1101 76-77 0.38367558936119295 1101 78-79 0.4390901005752603 1101 80-81 0.4355642353038718 1101 82-83 0.5027149802479087 1101 84-85 0.45723739091388893 1101 86-87 0.3434376799748158 1101 88-89 0.4312900715411452 1101 90-91 0.40281306518059523 1101 92-93 0.3209683217215904 1101 94-95 0.4875320020560032 1101 96-97 0.5509362065402854 1101 98-99 0.5939613773949937 1101 100-101 0.7905662476797666 1102 1 0.34205653880682974 1102 2 0.44329153179234027 1102 3 0.46578678111142935 1102 4 0.21369661170771082 1102 5 0.3218943800496703 1102 6 0.4214942376261419 1102 7 0.41475663317994815 1102 8 0.34809194346295413 1102 9 0.5710805268822625 1102 10-11 0.4857619808667053 1102 12-13 0.5567127835596395 1102 14-15 0.649933075743256 1102 16-17 0.557247836667436 1102 18-19 0.6042896559981159 1102 20-21 0.5787893227937886 1102 22-23 0.5984890732415522 1102 24-25 0.6461693852899728 1102 26-27 0.6639965722492107 1102 28-29 0.6495292508757657 1102 30-31 0.6081057331591282 1102 32-33 0.6747330335073585 1102 34-35 0.7570802200182669 1102 36-37 0.6820639246548907 1102 38-39 0.6039775969616699 1102 40-41 0.7078331985364628 1102 42-43 0.7530540479449925 1102 44-45 0.7953265855661371 1102 46-47 0.7233915939038056 1102 48-49 0.6623758898906331 1102 50-51 0.5185125958352117 1102 52-53 0.6198033616306233 1102 54-55 0.7327223290625753 1102 56-57 0.7288063449162507 1102 58-59 0.7120177277937145 1102 60-61 0.603836591857231 1102 62-63 0.475782603319729 1102 64-65 0.6568670114770754 1102 66-67 0.5484520845566863 1102 68-69 0.6442963527454424 1102 70-71 0.5458837478181735 1102 72-73 0.5027815695788433 1102 74-75 0.5956650198023539 1102 76-77 0.6901503995259013 1102 78-79 0.6487689262121457 1102 80-81 0.6470262840982812 1102 82-83 0.5604132174350163 1102 84-85 0.6794956778782684 1102 86-87 0.6805288801777287 1102 88-89 0.7327245367525386 1102 90-91 0.607387937170575 1102 92-93 0.6696363041568709 1102 94-95 0.8267662802063178 1102 96-97 0.8393612077292332 1102 98-99 1.0279359497701925 1102 100-101 1.1781322342684355 1103 1 0.36401736578758914 1103 2 0.43311123420031095 1103 3 0.3709035841887456 1103 4 0.23628255665365572 1103 5 0.26048443681334277 1103 6 0.34106928219942745 1103 7 0.3345151574803893 1103 8 0.35895526919971843 1103 9 0.5143332305272068 1103 10-11 0.480875492617983 1103 12-13 0.4691426478977405 1103 14-15 0.5092865056637521 1103 16-17 0.5740124436815464 1103 18-19 0.47353266271022676 1103 20-21 0.5596281702681267 1103 22-23 0.547309830901682 1103 24-25 0.47841324681093766 1103 26-27 0.5288424999741821 1103 28-29 0.4696274758995145 1103 30-31 0.4718228998421736 1103 32-33 0.3997985090571703 1103 34-35 0.42372504850274595 1103 36-37 0.4886436324600041 1103 38-39 0.4713394002651654 1103 40-41 0.5571405771902604 1103 42-43 0.5984908037894385 1103 44-45 0.5303164425104399 1103 46-47 0.5656463121210606 1103 48-49 0.3597488930618482 1103 50-51 0.44762985361467855 1103 52-53 0.5753270763366132 1103 54-55 0.5215250741220743 1103 56-57 0.5405437633935364 1103 58-59 0.5623904977643477 1103 60-61 0.5035027211486351 1103 62-63 0.35555746336037686 1103 64-65 0.35054936185823493 1103 66-67 0.39433896043313155 1103 68-69 0.3141807390691227 1103 70-71 0.33169261458809984 1103 72-73 0.273575028731301 1103 74-75 0.3669450609517284 1103 76-77 0.3951538975347262 1103 78-79 0.5304695434527602 1103 80-81 0.49966525293051234 1103 82-83 0.45715770992346094 1103 84-85 0.3644867242235712 1103 86-87 0.37427957237011 1103 88-89 0.41719585386010394 1103 90-91 0.44239998326785823 1103 92-93 0.30914114677377214 1103 94-95 0.4253343778978689 1103 96-97 0.5307170117721043 1103 98-99 0.5400744693598192 1103 100-101 0.7032886502483713 1104 1 0.3825616759073327 1104 2 0.45693578463228945 1104 3 0.43012630995317735 1104 4 0.16530651528639595 1104 5 0.28511239668662824 1104 6 0.30272091340444973 1104 7 0.2828349122692373 1104 8 0.3605842862947668 1104 9 0.37721334142641894 1104 10-11 0.3968773929890901 1104 12-13 0.4352986982693068 1104 14-15 0.5738745011001001 1104 16-17 0.5759347259537648 1104 18-19 0.5625808556672993 1104 20-21 0.5424633706114648 1104 22-23 0.5555254977621331 1104 24-25 0.4614086506327837 1104 26-27 0.554285064076943 1104 28-29 0.5712078355335208 1104 30-31 0.6475888242848171 1104 32-33 0.6059447284273105 1104 34-35 0.5837311670445189 1104 36-37 0.5861755931018138 1104 38-39 0.6083764925851085 1104 40-41 0.5827718898866578 1104 42-43 0.5452685163759057 1104 44-45 0.7272659679853817 1104 46-47 0.6042005884727359 1104 48-49 0.518286261775053 1104 50-51 0.47578142887766006 1104 52-53 0.4386638133958556 1104 54-55 0.6242800253759455 1104 56-57 0.6209369179751221 1104 58-59 0.6401972097638478 1104 60-61 0.5870233359523667 1104 62-63 0.5355804053405038 1104 64-65 0.5269145423545751 1104 66-67 0.3482885135920526 1104 68-69 0.32762839064496063 1104 70-71 0.4560082813337445 1104 72-73 0.37729199820582693 1104 74-75 0.42800542235648464 1104 76-77 0.6140164075478403 1104 78-79 0.7325014070730624 1104 80-81 0.6564909672377368 1104 82-83 0.6339301765875511 1104 84-85 0.6091860717619717 1104 86-87 0.5826217881778142 1104 88-89 0.6640843087356636 1104 90-91 0.6396625162956724 1104 92-93 0.56665406160446 1104 94-95 0.7366646899779461 1104 96-97 0.7865044939959915 1104 98-99 0.8460352379742027 1104 100-101 1.0955643612322383 1105 1 0.24222022136499177 1105 2 0.34177859560238844 1105 3 0.35915037999025046 1105 4 0.1503824624704535 1105 5 0.19387578413449802 1105 6 0.31128876845333764 1105 7 0.27798733046234503 1105 8 0.2954478580918263 1105 9 0.3823191782501567 1105 10-11 0.3921534834718301 1105 12-13 0.3854359838094652 1105 14-15 0.4689936424585852 1105 16-17 0.47532062329572256 1105 18-19 0.40827050752639593 1105 20-21 0.4141616769272005 1105 22-23 0.35617701826178916 1105 24-25 0.4424445743643588 1105 26-27 0.41946822738722034 1105 28-29 0.4177909034518663 1105 30-31 0.2646055300244896 1105 32-33 0.3982747465993839 1105 34-35 0.3925998278168237 1105 36-37 0.3961381802194879 1105 38-39 0.4435070105544128 1105 40-41 0.3084073949797883 1105 42-43 0.4036461702623626 1105 44-45 0.35685999060296325 1105 46-47 0.3671471554725585 1105 48-49 0.28878561018635907 1105 50-51 0.30963892814833116 1105 52-53 0.2643148038714074 1105 54-55 0.36909332113274473 1105 56-57 0.43104952360954485 1105 58-59 0.52387923696282 1105 60-61 0.44758010178126995 1105 62-63 0.3064138859027494 1105 64-65 0.2789987768838529 1105 66-67 0.44625252675351135 1105 68-69 0.2113473645530206 1105 70-71 0.21334049833094326 1105 72-73 0.3421998465423144 1105 74-75 0.5383208877405821 1105 76-77 0.47891964994391856 1105 78-79 0.48311831172985364 1105 80-81 0.4032939920573675 1105 82-83 0.5137410441520736 1105 84-85 0.6712052453682347 1105 86-87 0.5797475697773855 1105 88-89 0.6754565801809065 1105 90-91 0.7375586801734215 1105 92-93 0.8291112086911738 1105 94-95 0.8320285474920901 1105 96-97 0.79681141700339 1105 98-99 0.8237754036715152 1105 100-101 0.9335880668854948 1106 1 0.25590983568643466 1106 2 0.2903270761891399 1106 3 0.34831969659491335 1106 4 0.09435503705552861 1106 5 0.13952376614032147 1106 6 0.2365582076825845 1106 7 0.2799825778938114 1106 8 0.30472090908841665 1106 9 0.4899921054675076 1106 10-11 0.3000915199543712 1106 12-13 0.29145671221333913 1106 14-15 0.40250848788464566 1106 16-17 0.377962247759676 1106 18-19 0.33011523386112174 1106 20-21 0.3779828066898787 1106 22-23 0.3480107060748523 1106 24-25 0.40381263516398036 1106 26-27 0.4841440343193213 1106 28-29 0.40275264966285107 1106 30-31 0.408974036521748 1106 32-33 0.4561328808121061 1106 34-35 0.45113747772018087 1106 36-37 0.36304570835270766 1106 38-39 0.46599164784198166 1106 40-41 0.45485486154668564 1106 42-43 0.41498721208635203 1106 44-45 0.44987916483839996 1106 46-47 0.4615807846290281 1106 48-49 0.39834871104103087 1106 50-51 0.25984722828527396 1106 52-53 0.2636912890490777 1106 54-55 0.32502814304998395 1106 56-57 0.42305063669896725 1106 58-59 0.43728861601133673 1106 60-61 0.4282349770898577 1106 62-63 0.19540053575760652 1106 64-65 0.23372871413978658 1106 66-67 0.06921022704609925 1106 68-69 0.06304667337529679 1106 70-71 0.10465551418437968 1106 72-73 0.28116120940715206 1106 74-75 0.48985069290374383 1106 76-77 0.4336848042407446 1106 78-79 0.3461396105343191 1106 80-81 0.29327480165672526 1106 82-83 0.31137353527853406 1106 84-85 0.4550765334343936 1106 86-87 0.3925285844438413 1106 88-89 0.40685678663359326 1106 90-91 0.4331997342367764 1106 92-93 0.4860239096881003 1106 94-95 0.6839726436149824 1106 96-97 0.6177881876122875 1106 98-99 0.7023331907943167 1106 100-101 0.9847959536552047 1107 1 0.1772866304078491 1107 2 0.21242095907419767 1107 3 0.1578327994316382 1107 4 0.13123905432094318 1107 5 0.14831679749379845 1107 6 0.16998167122447683 1107 7 0.2092654121153501 1107 8 0.19438040802654655 1107 9 0.24841986207907496 1107 10-11 0.28942178653216644 1107 12-13 0.3306688102292128 1107 14-15 0.2674020076293644 1107 16-17 0.3392423974786567 1107 18-19 0.3390097102084866 1107 20-21 0.3438703661015907 1107 22-23 0.2826493266266681 1107 24-25 0.3758934326756531 1107 26-27 0.3162779761528398 1107 28-29 0.2904641379173114 1107 30-31 0.41326146699584143 1107 32-33 0.40802976273376856 1107 34-35 0.3633206152216246 1107 36-37 0.34109969382515004 1107 38-39 0.4744527545710895 1107 40-41 0.4426931651389978 1107 42-43 0.42089824755534977 1107 44-45 0.4274174968571387 1107 46-47 0.37145142498039263 1107 48-49 0.4049922970009092 1107 50-51 0.40363771532796733 1107 52-53 0.4584934488754513 1107 54-55 0.48336819491608907 1107 56-57 0.49497416718364917 1107 58-59 0.4198851906753376 1107 60-61 0.5143244247730934 1107 62-63 0.22435322807015723 1107 64-65 0.2162896951328861 1107 66-67 0.3559122133063397 1107 68-69 0.2910614269821963 1107 70-71 0.24679038011933585 1107 72-73 0.21951418414062118 1107 74-75 0.3108253215896042 1107 76-77 0.38939245250202603 1107 78-79 0.4316468258019306 1107 80-81 0.5028340853107522 1107 82-83 0.5407778366918023 1107 84-85 0.5430258868544406 1107 86-87 0.569618436209236 1107 88-89 0.5736562474046387 1107 90-91 0.5945643992172585 1107 92-93 0.4787473991339404 1107 94-95 0.6809533665194074 1107 96-97 0.7429577881191101 1107 98-99 0.7902830293069876 1107 100-101 1.0376042275889468 1108 1 0.02140146509593066 1108 2 0.09375636829846812 1108 3 0.023831669589785776 1108 4 -0.03402208134424001 1108 5 -0.04399694062859538 1108 6 0.05314509298147385 1108 7 0.09533191317505896 1108 8 0.04543922364661057 1108 9 0.143886274918259 1108 10-11 0.0862689908990859 1108 12-13 0.011527059574085285 1108 14-15 0.0843599495566636 1108 16-17 0.06341032409311254 1108 18-19 0.05772896724073462 1108 20-21 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-0.6826543670919349 2316 88-89 -0.7519263511212237 2316 90-91 -0.7554503838431721 2316 92-93 -0.6531288967411015 2316 94-95 -1.0604308477629765 2316 96-97 -0.7352053030639176 2316 98-99 -0.8923361420020406 2316 100-101 -1.2270234590252613 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 18.0 4 41.0 5 83.0 6 150.0 7 475.0 8 1144.0 9 2145.0 10 3807.0 11 6144.0 12 8747.0 13 11423.0 14 14041.0 15 17373.0 16 20386.0 17 23346.0 18 20211.0 19 16820.0 20 16636.0 21 17863.0 22 20169.0 23 23846.0 24 29361.0 25 37730.0 26 49263.0 27 64092.0 28 82119.0 29 103660.0 30 127986.0 31 156738.0 32 189723.0 33 231680.0 34 288714.0 35 362844.0 36 478391.0 37 658358.0 38 625947.0 39 108369.0 40 26.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.47823813836055 21.218099807654397 14.663740772476908 23.639921281508144 2 14.891665726344527 21.700077992194498 40.66150157097067 22.7467547104903 3 18.685843986187724 26.07326580780619 30.50366643192427 24.73722377408182 4 12.495922184261676 17.918394384310137 39.305711879109936 30.279971552318248 5 12.379100377218604 38.67824528031696 36.06700977354579 12.875644568918643 6 29.82295116300378 39.408065761744574 17.831354634344283 12.937628440907364 7 26.18513195475239 33.1931976742664 22.892847138776972 17.72882323220424 8 24.343990504309346 37.30456631930464 20.635691880126853 17.71575129625916 9 24.913550790824722 17.029571401981165 21.265314318041465 36.79156348915265 10-11 23.740069368798387 27.487772499242773 29.197842441358652 19.574315690600187 12-13 24.374776334187025 24.75455335818756 29.485221105518427 21.385449202106983 14-15 21.392713841533475 26.62786171163282 27.06415893931552 24.915265507518182 16-17 20.928156512009693 29.85004998857292 27.806029153325255 21.415764346092132 18-19 20.835391647837508 28.76430708560206 29.8605215408994 20.539779725661038 20-21 21.473802924861637 28.023169880255182 29.86701390334181 20.63601329154137 22-23 21.175208453402135 27.787521448765705 29.763942826678004 21.273327271154155 24-25 21.103177837119894 28.038993189909593 29.817219095060494 21.040609877910015 26-27 21.033771984422657 28.295069989005906 29.61335323501088 21.057804791560557 28-29 21.020193122008237 28.31471792851318 29.699099313719202 20.96598963575938 30-31 21.354614122611203 28.060492302061473 29.598613895887464 20.98627967943986 32-33 21.207054878580458 28.183641347765963 29.449996609087485 21.15930716456609 34-35 21.32839078267365 28.172748219887122 29.587046279203754 20.91181471823547 36-37 21.37571117133756 27.97701737702536 29.6119669855498 21.03530446608728 38-39 21.19301137239739 28.215200388076745 29.574293633043908 21.01749460648196 40-41 21.397536760042687 28.114630937457175 29.52160011246643 20.96623219003371 42-43 21.29911969906524 28.119674846983155 29.296865437470093 21.284340016481508 44-45 21.282895275097342 28.319844800604248 29.271147979403615 21.12611194489479 46-47 21.341779509252568 28.26875647993968 29.03320609088148 21.35625791992627 48-49 21.357528484642046 28.381512568299716 28.97906921914962 21.28188972790862 50-51 21.481904652489824 28.46715089145913 28.76687662156354 21.2840678344875 52-53 21.554786756563733 28.44626160569785 28.63968444295926 21.359267194779154 54-55 21.37778844902575 28.404037187960913 28.874110315975127 21.34406404703821 56-57 21.390101956116993 28.53010635706279 28.697138089295738 21.382653597524477 58-59 21.29522846464797 28.488043046942412 28.943219348553797 21.273509139855825 60-61 21.371818757509484 28.575234799128534 28.86266583769661 21.190280605665375 62-63 21.338946664605864 28.506716028014 28.830991004797145 21.323346302582998 64-65 21.135853606467528 28.427529430690214 28.871159714643774 21.565457248198484 66-67 21.158777804927404 28.438024010009027 28.783271887985833 21.619926297077736 68-69 21.29455422483555 28.474393007530736 28.737004265973127 21.494048501660593 70-71 21.30040092047573 28.194955174763255 28.78159510377657 21.72304880098445 72-73 21.342452477435074 28.40774824827161 28.71336001984444 21.536439254448876 74-75 21.261103961802213 28.60750451666559 28.55954201719719 21.571849504335 76-77 21.138520456787155 28.52638484578849 28.634018660988115 21.70107603643624 78-79 21.1586419372502 28.464053686432685 28.665262615548524 21.712041760768592 80-81 21.29979554278025 28.526965577362578 28.463638814933496 21.709600064923677 82-83 21.354165439531627 28.470946547175785 28.636946830431278 21.537941182861314 84-85 21.36391707263566 28.457412065793807 28.473839313505973 21.704831548064558 86-87 21.132391050697237 28.453262713184447 28.632129916361095 21.78221631975723 88-89 21.259021903886783 28.42878160775105 28.48723909452416 21.82495739383801 90-91 21.16874086299413 28.40161041691974 28.631239868133896 21.79840885195223 92-93 21.28920766109368 28.358902823717475 28.57544216115161 21.776447354037234 94-95 20.97179533195079 28.313182279953363 28.669341954782425 22.045680433313425 96-97 21.016953190304072 28.209421208203107 28.728273808199283 22.045351793293545 98-99 20.88070729053034 28.298754958607265 28.614878033903224 22.205659716959175 100-101 20.947581241575307 28.207888063294252 28.558933765599015 22.285596929531426 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 165.0 1 118.0 2 95.5 3 144.0 4 143.5 5 135.0 6 276.0 7 564.0 8 903.0 9 1104.0 10 1050.0 11 981.0 12 1078.0 13 1309.5 14 1587.0 15 1818.5 16 2129.0 17 2543.0 18 2875.5 19 3295.5 20 3850.5 21 4542.5 22 5466.0 23 7030.0 24 9041.5 25 11717.0 26 16141.5 27 21541.5 28 27570.5 29 36747.5 30 47877.5 31 59678.0 32 74544.5 33 88592.0 34 101187.5 35 113137.0 36 128383.5 37 144355.5 38 151895.0 39 156034.5 40 159847.0 41 160210.5 42 160241.5 43 163527.5 44 166125.5 45 169247.5 46 169391.0 47 165471.0 48 162059.5 49 156279.0 50 147486.0 51 136110.0 52 123382.0 53 110784.0 54 98483.0 55 83822.0 56 67274.5 57 52522.5 58 41138.0 59 31412.5 60 22184.0 61 15330.0 62 10603.5 63 6686.0 64 3779.0 65 2132.5 66 1170.0 67 673.0 68 379.0 69 192.5 70 111.0 71 76.5 72 54.0 73 36.5 74 25.0 75 17.0 76 10.5 77 10.5 78 12.0 79 5.5 80 1.5 81 1.0 82 0.5 83 0.5 84 0.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.004869271831812209 2 0.006806509012210616 3 0.002172847107744158 4 0.1716549215117885 5 0.0018848794187660165 6 6.021142587724775E-4 7 7.853664244858402E-5 8 9.162608285668136E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 3.795937718348228E-4 24-25 6.937403416291589E-4 26-27 0.0024739042371303968 28-29 2.094310465295574E-4 30-31 0.006453094121191988 32-33 0.022042617647235915 34-35 0.013678465226461716 36-37 7.984558648939376E-4 38-39 2.6178880816194676E-4 40-41 0.0017408955742769458 42-43 0.011531796999533755 44-45 0.00708138726078066 46-47 0.010288300160764508 48-49 0.010536999528518356 50-51 0.19339648202963816 52-53 0.11513471782962417 54-55 0.01780163895501238 56-57 0.006269841955478625 58-59 0.018155053846031007 60-61 0.1229622031936664 62-63 0.23728537571798855 64-65 0.24261277796408415 66-67 0.2542492904868827 68-69 0.24838522118405507 70-71 0.23606805776003548 72-73 0.16259702874938511 74-75 0.2787265440500247 76-77 0.21829259768583928 78-79 0.005536833292625174 80-81 7.853664244858402E-5 82-83 0.0 84-85 0.0 86-87 0.0 88-89 7.853664244858402E-5 90-91 0.006309110276702916 92-93 0.018469200415825344 94-95 0.002552440879578981 96-97 5.235776163238935E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3819873.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.47811624815828 #Duplication Level Percentage of deduplicated Percentage of total 1 70.95128151068974 36.524383175630895 2 16.038316532917694 16.51244645812592 3 5.546115925030142 8.565108009433905 4 2.5376159585353166 5.225267572266505 5 1.3733262526282095 3.5348124239721272 6 0.8351459030072665 2.579504272750871 7 0.5531413381375859 1.9932271874415934 8 0.3863387408647088 1.5910392490720464 9 0.2727545250120247 1.263680023120218 >10 1.3501801996292357 12.557080385528794 >50 0.10107822505539586 3.5898899539396316 >100 0.05235943515414914 4.808421501473918 >500 0.0018355721902106805 0.6538569939074921 >1k 4.588930335439321E-4 0.4318275063717486 >5k 5.098811483821429E-5 0.16945528696425952 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6456 0.16901085454935283 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4972 0.1301613954181199 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 7.853664244858402E-5 0.0 0.0 0.0 0.0 7 7.853664244858402E-5 0.0 0.0 0.0 2.6178880816194676E-5 8 7.853664244858402E-5 0.0 0.0 0.0 2.6178880816194676E-5 9 7.853664244858402E-5 0.0 0.0 0.0 2.6178880816194676E-5 10-11 7.853664244858402E-5 0.0 0.0 0.0 2.6178880816194676E-5 12-13 7.853664244858402E-5 0.0 0.0 0.0 2.6178880816194676E-5 14-15 7.853664244858402E-5 0.0 0.0 0.0 2.6178880816194676E-5 16-17 7.853664244858402E-5 0.0 0.0 0.0 2.6178880816194676E-5 18-19 7.853664244858402E-5 0.0 0.0 0.0 2.6178880816194676E-5 20-21 7.853664244858402E-5 0.0 0.0 2.6178880816194676E-5 2.6178880816194676E-5 22-23 7.853664244858402E-5 0.0 0.0 6.544720204048669E-5 2.6178880816194676E-5 24-25 7.853664244858402E-5 0.0 0.0 1.1780496367287603E-4 2.6178880816194676E-5 26-27 7.853664244858402E-5 0.0 0.0 2.0943104652955738E-4 2.6178880816194676E-5 28-29 7.853664244858402E-5 0.0 0.0 6.806509012210615E-4 2.6178880816194676E-5 30-31 9.162608285668136E-5 0.0 0.0 0.003206912899983848 2.6178880816194676E-5 32-33 1.1780496367287603E-4 0.0 0.0 0.009620738699951543 2.6178880816194676E-5 34-35 1.3089440408097338E-4 0.0 0.0 0.01922838795949499 2.6178880816194676E-5 36-37 1.3089440408097338E-4 0.0 0.0 0.03473937484309034 2.6178880816194676E-5 38-39 1.5707328489716804E-4 0.0 0.0 0.05984492154582102 2.6178880816194676E-5 40-41 1.5707328489716804E-4 0.0 0.0 0.08889038981138903 2.6178880816194676E-5 42-43 1.5707328489716804E-4 0.0 0.0 0.11932333876021532 2.6178880816194676E-5 44-45 1.5707328489716804E-4 0.0 0.0 0.15721726874165712 2.6178880816194676E-5 46-47 1.5707328489716804E-4 0.0 0.0 0.20021608048225686 2.6178880816194676E-5 48-49 1.7016272530526538E-4 0.0 0.0 0.24790091188895547 2.6178880816194676E-5 50-51 2.6178880816194676E-4 0.0 0.0 0.2975360699164606 2.6178880816194676E-5 52-53 2.6178880816194676E-4 0.0 0.0 0.3496582216215042 2.6178880816194676E-5 54-55 2.6178880816194676E-4 0.0 0.0 0.40793241031835353 2.6178880816194676E-5 56-57 2.6178880816194676E-4 0.0 0.0 0.4778954692996338 2.6178880816194676E-5 58-59 2.6178880816194676E-4 0.0 0.0 0.5548613788992461 2.6178880816194676E-5 60-61 2.6178880816194676E-4 0.0 0.0 0.6380055043714804 2.6178880816194676E-5 62-63 2.8796768897814144E-4 0.0 0.0 0.7265293898514427 2.6178880816194676E-5 64-65 2.8796768897814144E-4 0.0 0.0 0.8202366937330114 2.6178880816194676E-5 66-67 2.8796768897814144E-4 0.0 0.0 0.9283947398251198 2.6178880816194676E-5 68-69 3.010571293862388E-4 0.0 0.0 1.0523517404898017 2.6178880816194676E-5 70-71 3.4032545061053076E-4 0.0 0.0 1.1883902946511573 2.6178880816194676E-5 72-73 3.6650433142672545E-4 0.0 0.0 1.3312746261459476 2.6178880816194676E-5 74-75 3.6650433142672545E-4 0.0 0.0 1.4779548953590864 2.6178880816194676E-5 76-77 3.6650433142672545E-4 0.0 0.0 1.6439028208529445 2.6178880816194676E-5 78-79 3.6650433142672545E-4 0.0 0.0 1.8338567800552532 2.6178880816194676E-5 80-81 3.6650433142672545E-4 0.0 0.0 2.0426857123260382 2.6178880816194676E-5 82-83 3.6650433142672545E-4 0.0 0.0 2.2669209159571535 2.6178880816194676E-5 84-85 3.6650433142672545E-4 0.0 0.0 2.497609213709461 2.6178880816194676E-5 86-87 3.6650433142672545E-4 0.0 0.0 2.7426304487086353 2.6178880816194676E-5 88-89 3.6650433142672545E-4 0.0 0.0 3.0218805703749836 2.6178880816194676E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3910 0.0 55.17142 1 GTATCAA 7935 0.0 43.05435 1 CAACGCA 9755 0.0 34.189426 5 ATCAACG 9770 0.0 34.13708 3 TCAACGC 10050 0.0 33.32782 4 TATCAAC 10405 0.0 32.33111 2 AACGCAG 10470 0.0 31.944822 6 ACGCAGA 12025 0.0 27.65538 7 CGCAGAG 12190 0.0 27.164291 8 GTACATG 10040 0.0 23.615759 1 GCAGAGT 14155 0.0 22.82359 9 TACATGG 10150 0.0 22.704144 2 ACATGGG 9790 0.0 22.226273 3 AGAGTAC 13270 0.0 20.162987 10-11 GAGTACT 9495 0.0 19.13597 12-13 GAGTACA 7710 0.0 19.043169 1 GGCGTCG 625 0.0 18.976088 8 GCGTCGG 575 0.0 18.976088 9 ACGGGCG 585 0.0 18.652443 5 GTGGTAT 2145 0.0 18.60746 1 >>END_MODULE