FastQCFastQC Report
Sat 18 Jun 2016
SRR1931003_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931003_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5417988
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2440554.504531940639219No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32700.047.8576551
GTATCAA66600.045.1463621
ATCAACG74800.038.902573
CAACGCA77100.038.1892855
TCAACGC79400.037.280814
TATCAAC81000.036.0383642
AACGCAG86950.033.6892246
ACGCAGA97400.028.8724277
CGCAGAG98850.028.0753388
GTACATG91250.025.4720951
TACATGG93150.024.2330232
GCAGAGT113200.023.3912419
ACATGGG94400.022.6515483
GTGTAGC23800.022.2054541
GTGGTAT21350.019.7067641
GTATAAT25300.019.672091
AGAGTAC97400.019.53269410-11
GTACTAG11600.018.5776441
CAGAGTA107550.018.54077110-11
GAGTACA66700.018.3853281
Produced by FastQC (version 0.11.5)