##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931003_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5417988 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.80712508038039 30.0 2.0 31.0 2.0 33.0 2 20.406932979548866 28.0 2.0 31.0 2.0 33.0 3 21.1531099736655 28.0 2.0 31.0 2.0 34.0 4 22.64937445413316 32.0 2.0 35.0 2.0 37.0 5 21.974663657431503 32.0 2.0 35.0 2.0 37.0 6 23.026774883960613 32.0 2.0 35.0 2.0 37.0 7 23.255640285655858 32.0 2.0 35.0 2.0 37.0 8 23.74877426823389 32.0 2.0 35.0 2.0 37.0 9 25.1753444267503 33.0 8.0 37.0 2.0 39.0 10-11 25.605119649582093 33.5 5.0 37.0 2.0 39.0 12-13 25.558838908465653 34.0 2.0 37.0 2.0 39.0 14-15 25.587117210300207 33.5 2.0 38.0 2.0 40.0 16-17 24.39289317362829 32.5 2.0 38.0 2.0 40.0 18-19 23.265732223843983 31.5 2.0 38.0 2.0 40.0 20-21 22.919321526736496 31.0 2.0 38.0 2.0 40.0 22-23 22.55803888823674 30.0 2.0 38.0 2.0 40.0 24-25 22.41871595138269 30.0 2.0 38.0 2.0 40.0 26-27 22.143621488272032 29.5 2.0 38.0 2.0 40.0 28-29 21.976463403019718 29.0 2.0 37.5 2.0 40.0 30-31 22.02584797160865 30.0 2.0 37.0 2.0 40.0 32-33 21.943988617176707 29.5 2.0 37.0 2.0 40.0 34-35 21.654650582467145 28.5 2.0 37.0 2.0 40.0 36-37 21.20970469111412 27.0 2.0 37.0 2.0 39.5 38-39 21.130490875948784 27.0 2.0 37.0 2.0 39.0 40-41 21.04867867924403 27.0 2.0 37.0 2.0 39.0 42-43 20.946368467408938 26.5 2.0 37.0 2.0 39.0 44-45 20.585286456891374 26.0 2.0 36.0 2.0 39.0 46-47 20.29477861523503 25.0 2.0 36.0 2.0 39.0 48-49 20.1097124061552 24.0 2.0 36.0 2.0 39.0 50-51 17.608038998978955 12.0 2.0 33.5 2.0 38.0 52-53 19.66927759899062 24.5 2.0 35.5 2.0 38.5 54-55 20.544050762017193 26.5 2.0 37.0 2.0 39.0 56-57 20.549447783937506 26.5 2.0 37.0 2.0 39.0 58-59 20.28656440361256 26.0 2.0 36.5 2.0 39.0 60-61 20.009321726072486 26.0 2.0 36.0 2.0 39.0 62-63 19.57222856529029 24.5 2.0 36.0 2.0 39.0 64-65 19.233842156904004 23.0 2.0 35.0 2.0 39.0 66-67 18.884936253088785 22.5 2.0 34.5 2.0 38.5 68-69 18.437452888415404 21.5 2.0 34.0 2.0 38.0 70-71 18.09530465922036 20.0 2.0 34.0 2.0 38.0 72-73 17.69865843187545 18.5 2.0 34.0 2.0 37.0 74-75 17.246136480922438 15.0 2.0 33.0 2.0 36.0 76-77 16.836401538726186 12.0 2.0 32.5 2.0 36.0 78-79 16.469979446244622 8.0 2.0 32.0 2.0 35.5 80-81 16.036312557355238 7.0 2.0 32.0 2.0 35.0 82-83 15.580275187025148 2.0 2.0 31.0 2.0 35.0 84-85 15.192271097684234 2.0 2.0 31.0 2.0 34.0 86-87 14.713576423572736 2.0 2.0 31.0 2.0 34.0 88-89 14.29830317084497 2.0 2.0 31.0 2.0 34.0 90-91 13.80670988935376 2.0 2.0 30.0 2.0 34.0 92-93 13.250622555826997 2.0 2.0 29.5 2.0 34.0 94-95 12.663949698670429 2.0 2.0 29.0 2.0 34.0 96-97 11.99082565336062 2.0 2.0 29.0 2.0 34.0 98-99 11.262414756178861 2.0 2.0 28.0 2.0 34.0 100-101 10.186936091405148 2.0 2.0 24.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.932902851448549 1101 2 9.606624377992397 1101 3 9.013028471872225 1101 4 11.090732985813897 1101 5 11.980920462232309 1101 6 11.091862794616645 1101 7 10.639091699761725 1101 8 10.077659538519573 1101 9 9.950654585244216 1101 10-11 9.383405153307038 1101 12-13 9.331428126881349 1101 14-15 10.104383066154742 1101 16-17 11.063535038953784 1101 18-19 12.13852582285018 1101 20-21 12.126493189464366 1101 22-23 12.308148987502097 1101 24-25 12.203396397035633 1101 26-27 12.35755489732929 1101 28-29 12.310369154932733 1101 30-31 11.909288224816049 1101 32-33 11.78446397515562 1101 34-35 11.751319534309388 1101 36-37 12.274548662906149 1101 38-39 11.93068222116867 1101 40-41 11.658215429449314 1101 42-43 11.366123088024633 1101 44-45 11.162656305862505 1101 46-47 10.928629163352849 1101 48-49 11.14148994194029 1101 50-51 13.002191654019047 1101 52-53 11.952282698489167 1101 54-55 11.636344115834408 1101 56-57 11.512027156842684 1101 58-59 11.53501084356592 1101 60-61 11.254050340378189 1101 62-63 11.295913309069654 1101 64-65 11.109876639318902 1101 66-67 10.78466651215949 1101 68-69 10.845742092260089 1101 70-71 10.68747633256158 1101 72-73 10.488799845039715 1101 74-75 10.314829331307362 1101 76-77 10.36192948194719 1101 78-79 9.990182960301336 1101 80-81 9.851475356404906 1101 82-83 9.559431843950179 1101 84-85 9.406710939018913 1101 86-87 9.251270180059558 1101 88-89 9.062081426898393 1101 90-91 8.75439925168435 1101 92-93 8.588897774535281 1101 94-95 8.35612865916683 1101 96-97 8.359001255266103 1101 98-99 7.684065374410549 1101 100-101 6.956407167063002 1102 1 8.062699339289612 1102 2 9.422316225446643 1102 3 8.824300517228629 1102 4 10.846129224127697 1102 5 11.532120153947854 1102 6 10.573120724349756 1102 7 10.341117691640097 1102 8 9.712554833679722 1102 9 9.530561494895643 1102 10-11 8.753587782955407 1102 12-13 8.867256587515328 1102 14-15 9.487799280003639 1102 16-17 10.517974919602576 1102 18-19 11.649129471164446 1102 20-21 11.677220691165285 1102 22-23 11.820928096052587 1102 24-25 11.633427348118502 1102 26-27 11.837946867162547 1102 28-29 11.538219794768768 1102 30-31 11.571233252509725 1102 32-33 11.48017745321167 1102 34-35 11.281639645141354 1102 36-37 11.484425695237725 1102 38-39 11.365923886662333 1102 40-41 11.096987295277447 1102 42-43 10.620996991045576 1102 44-45 10.632803888321849 1102 46-47 10.555007903008512 1102 48-49 10.216204464735064 1102 50-51 12.176371448278967 1102 52-53 11.311288654194808 1102 54-55 10.965468663760419 1102 56-57 10.954307556974754 1102 58-59 11.066067837898824 1102 60-61 10.757507605223065 1102 62-63 10.734697540886913 1102 64-65 10.56940558438625 1102 66-67 10.434825219239798 1102 68-69 10.270294501365978 1102 70-71 9.813362170022476 1102 72-73 9.736194879594308 1102 74-75 9.571732857651412 1102 76-77 9.292751788202118 1102 78-79 8.918538030295561 1102 80-81 8.587908401489193 1102 82-83 8.72374034260736 1102 84-85 8.466244626085397 1102 86-87 8.245231966359734 1102 88-89 8.170040736185337 1102 90-91 7.885665725191773 1102 92-93 7.517581752587455 1102 94-95 7.3395656260751725 1102 96-97 7.1775572429461665 1102 98-99 6.844478348029638 1102 100-101 6.006668951305585 1103 1 8.242329089846187 1103 2 9.686632869888623 1103 3 9.002380298727775 1103 4 11.090956895840907 1103 5 11.833247928544527 1103 6 10.888006830364297 1103 7 10.587058146226841 1103 8 10.100503566932584 1103 9 9.860219427619235 1103 10-11 9.335566826090464 1103 12-13 9.126380066979976 1103 14-15 9.997993635028362 1103 16-17 11.022182611044972 1103 18-19 11.89790351515136 1103 20-21 12.262539803994809 1103 22-23 12.3275075943933 1103 24-25 12.230033931817601 1103 26-27 12.213140588230548 1103 28-29 11.929765906615575 1103 30-31 11.712947762245289 1103 32-33 11.8228835320914 1103 34-35 11.928063848479653 1103 36-37 12.083344199147973 1103 38-39 11.814076703184693 1103 40-41 11.52141783203394 1103 42-43 11.24108537203238 1103 44-45 11.31501526640475 1103 46-47 11.112770150053262 1103 48-49 11.185535880669562 1103 50-51 13.197946863831422 1103 52-53 12.067272393622485 1103 54-55 11.614113887873977 1103 56-57 11.326774328385195 1103 58-59 11.203378186418622 1103 60-61 11.244396564677842 1103 62-63 11.113690306962763 1103 64-65 10.903403430470643 1103 66-67 10.934494171514856 1103 68-69 10.673421936560196 1103 70-71 10.532570370434879 1103 72-73 10.419477534264207 1103 74-75 10.210388684916822 1103 76-77 9.830061912815218 1103 78-79 9.400215377534547 1103 80-81 9.081831038984449 1103 82-83 8.93426330243469 1103 84-85 8.686787945381228 1103 86-87 8.54242142264008 1103 88-89 8.35662121904715 1103 90-91 8.2563543766445 1103 92-93 7.965809277083681 1103 94-95 7.772066044312835 1103 96-97 7.472749167413548 1103 98-99 6.99807018911015 1103 100-101 6.4352312016120194 1104 1 7.9659952332898705 1104 2 9.600062379961003 1104 3 8.859227095317742 1104 4 10.991679116057089 1104 5 11.775321338486354 1104 6 10.908251839551433 1104 7 10.533752318627304 1104 8 9.982305892143504 1104 9 9.883881155624763 1104 10-11 9.06755333493161 1104 12-13 8.86586744619666 1104 14-15 9.660255612499714 1104 16-17 10.637185368762868 1104 18-19 11.625060187962948 1104 20-21 11.795211198141086 1104 22-23 12.052341029069314 1104 24-25 11.873053239962218 1104 26-27 11.99219504017329 1104 28-29 11.65800627663187 1104 30-31 11.389320331123397 1104 32-33 11.56188851181739 1104 34-35 11.427266350192241 1104 36-37 11.613955221767952 1104 38-39 11.203012455148293 1104 40-41 11.01782552552941 1104 42-43 10.689018361257617 1104 44-45 10.807715842349097 1104 46-47 10.743132469579471 1104 48-49 10.781811773979964 1104 50-51 12.545550751656894 1104 52-53 11.592632021726736 1104 54-55 11.230704208010621 1104 56-57 10.955258718942414 1104 58-59 11.045138167835166 1104 60-61 10.833303133556065 1104 62-63 10.848306447371634 1104 64-65 10.760847341050425 1104 66-67 10.61017682818229 1104 68-69 10.295552022448192 1104 70-71 9.876083927664393 1104 72-73 9.78159281427411 1104 74-75 9.695495946272203 1104 76-77 9.398429099235596 1104 78-79 9.154384539149046 1104 80-81 8.976481999564305 1104 82-83 8.64140351003881 1104 84-85 8.233928202546386 1104 86-87 8.066331052395293 1104 88-89 7.76613825507552 1104 90-91 7.455232195613727 1104 92-93 7.246781725200195 1104 94-95 6.959438251652797 1104 96-97 6.335245842157379 1104 98-99 5.880010593310688 1104 100-101 5.217269405192939 1105 1 8.035597681846163 1105 2 9.223374720015293 1105 3 8.688545263502306 1105 4 10.85057857534797 1105 5 10.981116740689206 1105 6 10.637201241135926 1105 7 10.111785693265542 1105 8 9.750112897547854 1105 9 9.602158479518835 1105 10-11 8.909851774078092 1105 12-13 8.50220031966414 1105 14-15 9.34577715387869 1105 16-17 10.294924507904216 1105 18-19 10.97631407305541 1105 20-21 11.481370836950074 1105 22-23 11.72984472463175 1105 24-25 11.698736442261556 1105 26-27 11.824867835221998 1105 28-29 11.596667368478382 1105 30-31 11.394814298653383 1105 32-33 11.254739520335615 1105 34-35 11.201411586154862 1105 36-37 11.42327062963377 1105 38-39 11.376015823024307 1105 40-41 11.039850053914094 1105 42-43 10.756996812918548 1105 44-45 10.510733026176027 1105 46-47 10.505727875203068 1105 48-49 10.413177384565653 1105 50-51 12.18020302671323 1105 52-53 10.972662729180453 1105 54-55 10.890942646616534 1105 56-57 10.888361033196901 1105 58-59 10.690082102839959 1105 60-61 10.520669834411486 1105 62-63 10.604156878045657 1105 64-65 10.293012950936987 1105 66-67 10.052121120055972 1105 68-69 9.78656706631023 1105 70-71 9.67547376738176 1105 72-73 9.56479145254183 1105 74-75 9.367194514221083 1105 76-77 9.045465308412826 1105 78-79 8.776687727392108 1105 80-81 8.468931572716778 1105 82-83 8.385632917620846 1105 84-85 8.124153456291902 1105 86-87 7.872352305101851 1105 88-89 7.434752048217282 1105 90-91 7.249399636474271 1105 92-93 6.998039052888293 1105 94-95 6.7850792720119255 1105 96-97 6.519546822644822 1105 98-99 6.035315430316738 1105 100-101 5.4198987430116965 1106 1 7.963341266909566 1106 2 9.247405164609727 1106 3 8.791197112116379 1106 4 10.768414724710762 1106 5 11.37749809947972 1106 6 10.490254999570595 1106 7 9.741243033353218 1106 8 9.333542263422654 1106 9 9.14420023120983 1106 10-11 8.784770490018651 1106 12-13 8.536959496265133 1106 14-15 9.283900793154576 1106 16-17 10.349593241542951 1106 18-19 11.62565108875052 1106 20-21 11.654037137727077 1106 22-23 11.724999151283026 1106 24-25 11.42169019598181 1106 26-27 11.725234637417348 1106 28-29 11.702177446174073 1106 30-31 11.323536179746245 1106 32-33 11.51219166032114 1106 34-35 11.41677881747566 1106 36-37 11.597430100594345 1106 38-39 11.22804686048099 1106 40-41 11.090241526621611 1106 42-43 10.71140589281584 1106 44-45 10.497386353951452 1106 46-47 10.405114593982784 1106 48-49 10.549695129170292 1106 50-51 12.512845507285032 1106 52-53 11.652748561603008 1106 54-55 10.977866902415386 1106 56-57 10.858439012425151 1106 58-59 10.718054024194846 1106 60-61 10.6592858481633 1106 62-63 10.810409570391549 1106 64-65 10.693073651793409 1106 66-67 10.32170255424058 1106 68-69 9.960728064951297 1106 70-71 9.749829597225897 1106 72-73 9.534184526777903 1106 74-75 9.180772718644715 1106 76-77 8.971388799668702 1106 78-79 8.757754220693517 1106 80-81 8.4503550696709 1106 82-83 8.254870770353484 1106 84-85 8.039563160185567 1106 86-87 7.888192854612187 1106 88-89 7.605463895688041 1106 90-91 7.2791311707662505 1106 92-93 7.155330532827849 1106 94-95 7.011628742568282 1106 96-97 6.551462955308226 1106 98-99 6.077441128050429 1106 100-101 5.356684607763025 1107 1 7.519385116263532 1107 2 8.587123323286619 1107 3 8.366468313722248 1107 4 10.172302732924035 1107 5 10.985555067759574 1107 6 10.129757981932713 1107 7 10.025166198237553 1107 8 8.516489318109215 1107 9 5.824432699420708 1107 10-11 7.892126556089671 1107 12-13 7.807006570324049 1107 14-15 8.397839131790814 1107 16-17 9.6177310912723 1107 18-19 11.037560938724102 1107 20-21 11.314134768345337 1107 22-23 10.741521475090739 1107 24-25 10.88762341187497 1107 26-27 10.973869399254273 1107 28-29 10.952416178363006 1107 30-31 10.888499391124224 1107 32-33 11.145843891194335 1107 34-35 11.284240805832653 1107 36-37 11.38315664290107 1107 38-39 11.017118864569536 1107 40-41 10.707234018139609 1107 42-43 10.456756837651376 1107 44-45 10.050333989054757 1107 46-47 10.324923161989158 1107 48-49 10.613923653562491 1107 50-51 12.293905499008435 1107 52-53 11.043562780757814 1107 54-55 10.802724055825564 1107 56-57 10.42707805577776 1107 58-59 10.56430031838472 1107 60-61 10.387452417214423 1107 62-63 10.48430130985529 1107 64-65 10.406974753303771 1107 66-67 10.31416625746521 1107 68-69 10.114358104306568 1107 70-71 9.709192540858385 1107 72-73 9.356428824937229 1107 74-75 9.61389601649725 1107 76-77 9.465866624357218 1107 78-79 9.093941289762359 1107 80-81 8.670379628083063 1107 82-83 8.509499533545718 1107 84-85 8.444149688637667 1107 86-87 8.06081311959881 1107 88-89 7.9486058799444255 1107 90-91 7.731858760084473 1107 92-93 7.232524285734643 1107 94-95 7.040669961956011 1107 96-97 6.608985510528848 1107 98-99 6.297702360810353 1107 100-101 5.741052040202895 1108 1 6.88505383820414 1108 2 8.107678113501553 1108 3 8.044790211529214 1108 4 9.514244944505794 1108 5 11.21549751117266 1108 6 9.642191185529661 1108 7 9.032359746193205 1108 8 6.035895384065231 1108 9 7.361895840467781 1108 10-11 6.248360143991647 1108 12-13 5.153904622495013 1108 14-15 7.461930821083325 1108 16-17 8.767097612152721 1108 18-19 8.508700612644414 1108 20-21 8.8300626955503 1108 22-23 9.681707564319051 1108 24-25 9.263605418196832 1108 26-27 7.626001980027429 1108 28-29 9.580844629417616 1108 30-31 10.130082219555664 1108 32-33 10.640808826705094 1108 34-35 10.16073756688142 1108 36-37 9.95003788730034 1108 38-39 9.590462853227635 1108 40-41 9.87572323575344 1108 42-43 9.047541007137912 1108 44-45 8.45378399596511 1108 46-47 9.3778083475211 1108 48-49 10.070133345003903 1108 50-51 11.617755504135477 1108 52-53 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-18.780644769739176 2313 42-43 -18.682290781396244 2313 44-45 -18.322220123499356 2313 46-47 -18.0356828986169 2313 48-49 -17.85906335942452 2313 50-51 -15.436484111381173 2313 52-53 -17.44249782530279 2313 54-55 -18.286193693092187 2313 56-57 -18.30123363253393 2313 58-59 -18.045310071225668 2313 60-61 -17.773153643391638 2313 62-63 -17.33881557326159 2313 64-65 -17.001336935007917 2313 66-67 -16.647738504439552 2313 68-69 -16.220100770152307 2313 70-71 -15.880764169098324 2313 72-73 -15.498289789781385 2313 74-75 -15.04463212203089 2313 76-77 -14.634566276075681 2313 78-79 -14.27411065828959 2313 80-81 -13.842087904384469 2313 82-83 -13.386344622298438 2313 84-85 -13.007711783223803 2313 86-87 -12.537368698583007 2313 88-89 -12.124381133042588 2313 90-91 -11.642686694460982 2313 92-93 -11.085484372274015 2313 94-95 -10.497338696052646 2313 96-97 -9.848763407220067 2313 98-99 -9.12541452230653 2313 100-101 -8.058255319104465 2314 1 -19.53187029499188 2314 2 -18.16283338667009 2314 3 -18.890328223074288 2314 4 -20.37367129304999 2314 5 -19.668470906266403 2314 6 -20.724441152266422 2314 7 -20.956998303926948 2314 8 -21.462731461111563 2314 9 -22.876346521358514 2314 10-11 -23.325189461226344 2314 12-13 -23.29048260716513 2314 14-15 -23.310039656087977 2314 16-17 -22.10365419011298 2314 18-19 -20.97499317383001 2314 20-21 -20.640843586633014 2314 22-23 -20.27761272404346 2314 24-25 -20.13254974829819 2314 26-27 -19.872710687325817 2314 28-29 -19.700051354104527 2314 30-31 -19.749509266884434 2314 32-33 -19.66463229928186 2314 34-35 -19.392815471223628 2314 36-37 -18.94723296748854 2314 38-39 -18.86911490166423 2314 40-41 -18.780644769739176 2314 42-43 -18.682290781396244 2314 44-45 -18.322220123499356 2314 46-47 -18.0356828986169 2314 48-49 -17.85906335942452 2314 50-51 -15.436484111381173 2314 52-53 -17.44249782530279 2314 54-55 -18.286193693092187 2314 56-57 -18.30123363253393 2314 58-59 -18.045310071225668 2314 60-61 -17.773153643391638 2314 62-63 -17.33881557326159 2314 64-65 -17.001336935007917 2314 66-67 -16.647738504439552 2314 68-69 -16.220100770152307 2314 70-71 -15.880764169098324 2314 72-73 -15.498289789781385 2314 74-75 -15.04463212203089 2314 76-77 -14.634566276075681 2314 78-79 -14.27411065828959 2314 80-81 -13.842087904384469 2314 82-83 -13.386344622298438 2314 84-85 -13.007711783223803 2314 86-87 -12.537368698583007 2314 88-89 -12.124381133042588 2314 90-91 -11.642686694460982 2314 92-93 -11.085484372274015 2314 94-95 -10.497338696052646 2314 96-97 -9.848763407220067 2314 98-99 -9.12541452230653 2314 100-101 -8.058255319104465 2315 1 -19.53187029499188 2315 2 -18.16283338667009 2315 3 -18.890328223074288 2315 4 -20.37367129304999 2315 5 -19.668470906266403 2315 6 -20.724441152266422 2315 7 -20.956998303926948 2315 8 -21.462731461111563 2315 9 -22.876346521358514 2315 10-11 -23.325189461226344 2315 12-13 -23.29048260716513 2315 14-15 -23.310039656087977 2315 16-17 -22.10365419011298 2315 18-19 -20.97499317383001 2315 20-21 -20.640843586633014 2315 22-23 -20.27761272404346 2315 24-25 -20.13254974829819 2315 26-27 -19.872710687325817 2315 28-29 -19.700051354104527 2315 30-31 -19.749509266884434 2315 32-33 -19.66463229928186 2315 34-35 -19.392815471223628 2315 36-37 -18.94723296748854 2315 38-39 -18.86911490166423 2315 40-41 -18.780644769739176 2315 42-43 -18.682290781396244 2315 44-45 -18.322220123499356 2315 46-47 -18.0356828986169 2315 48-49 -17.85906335942452 2315 50-51 -15.436484111381173 2315 52-53 -17.44249782530279 2315 54-55 -18.286193693092187 2315 56-57 -18.30123363253393 2315 58-59 -18.045310071225668 2315 60-61 -17.773153643391638 2315 62-63 -17.33881557326159 2315 64-65 -17.001336935007917 2315 66-67 -16.647738504439552 2315 68-69 -16.220100770152307 2315 70-71 -15.880764169098324 2315 72-73 -15.498289789781385 2315 74-75 -15.04463212203089 2315 76-77 -14.634566276075681 2315 78-79 -14.27411065828959 2315 80-81 -13.842087904384469 2315 82-83 -13.386344622298438 2315 84-85 -13.007711783223803 2315 86-87 -12.537368698583007 2315 88-89 -12.124381133042588 2315 90-91 -11.642686694460982 2315 92-93 -11.085484372274015 2315 94-95 -10.497338696052646 2315 96-97 -9.848763407220067 2315 98-99 -9.12541452230653 2315 100-101 -8.058255319104465 2316 1 -19.53187029499188 2316 2 -18.16283338667009 2316 3 -18.890328223074288 2316 4 -20.37367129304999 2316 5 -19.668470906266403 2316 6 -20.724441152266422 2316 7 -20.956998303926948 2316 8 -21.462731461111563 2316 9 -22.876346521358514 2316 10-11 -23.325189461226344 2316 12-13 -23.29048260716513 2316 14-15 -23.310039656087977 2316 16-17 -22.10365419011298 2316 18-19 -20.97499317383001 2316 20-21 -20.640843586633014 2316 22-23 -20.27761272404346 2316 24-25 -20.13254974829819 2316 26-27 -19.872710687325817 2316 28-29 -19.700051354104527 2316 30-31 -19.749509266884434 2316 32-33 -19.66463229928186 2316 34-35 -19.392815471223628 2316 36-37 -18.94723296748854 2316 38-39 -18.86911490166423 2316 40-41 -18.780644769739176 2316 42-43 -18.682290781396244 2316 44-45 -18.322220123499356 2316 46-47 -18.0356828986169 2316 48-49 -17.85906335942452 2316 50-51 -15.436484111381173 2316 52-53 -17.44249782530279 2316 54-55 -18.286193693092187 2316 56-57 -18.30123363253393 2316 58-59 -18.045310071225668 2316 60-61 -17.773153643391638 2316 62-63 -17.33881557326159 2316 64-65 -17.001336935007917 2316 66-67 -16.647738504439552 2316 68-69 -16.220100770152307 2316 70-71 -15.880764169098324 2316 72-73 -15.498289789781385 2316 74-75 -15.04463212203089 2316 76-77 -14.634566276075681 2316 78-79 -14.27411065828959 2316 80-81 -13.842087904384469 2316 82-83 -13.386344622298438 2316 84-85 -13.007711783223803 2316 86-87 -12.537368698583007 2316 88-89 -12.124381133042588 2316 90-91 -11.642686694460982 2316 92-93 -11.085484372274015 2316 94-95 -10.497338696052646 2316 96-97 -9.848763407220067 2316 98-99 -9.12541452230653 2316 100-101 -8.058255319104465 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 1334793.0 3 45453.0 4 209376.0 5 99783.0 6 51394.0 7 52802.0 8 49309.0 9 45051.0 10 48698.0 11 52336.0 12 54867.0 13 57327.0 14 59466.0 15 61726.0 16 65047.0 17 67514.0 18 68017.0 19 70620.0 20 76282.0 21 82330.0 22 89762.0 23 97870.0 24 105785.0 25 115229.0 26 126381.0 27 138961.0 28 151904.0 29 166562.0 30 185214.0 31 204895.0 32 227731.0 33 251767.0 34 272104.0 35 275431.0 36 227821.0 37 111614.0 38 16602.0 39 164.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.18333192550988 19.928165312578447 14.004742183902986 23.883760578008687 2 14.926596800281303 20.641274793837216 41.32785260301065 23.104275802870834 3 19.038128116166252 25.400110454924192 29.66405057834891 25.897710850560646 4 12.589999611606867 16.980075432244817 39.17288533060129 31.25703962554703 5 13.253818644534343 37.624438006398606 35.48873870037795 13.633004648689099 6 30.346182533417622 37.72788617502281 18.288373123306684 13.637558168252884 7 26.78838149604515 32.50426283736599 22.80524181581521 17.902113850773645 8 24.63882190765335 36.08703719899512 20.921721956842333 18.352418936509192 9 25.381175191177373 17.315017659804823 21.221459174008057 36.082347975009746 10-11 24.119164306246567 27.017809063312942 28.94414820053709 19.9188784299034 12-13 24.82620446500024 24.34183560244774 28.96455694926921 21.867402983282812 14-15 21.99481840500146 26.265110151829703 26.64209383742443 25.097977605744408 16-17 21.75364025118984 29.15829896440964 27.232504565950805 21.855556218449713 18-19 21.47906620668613 28.412711789332235 28.910543160996315 21.19767884298532 20-21 22.460485597158435 27.799407001387305 28.65433010378064 21.085777297673623 22-23 21.924099397506616 27.5362367796135 28.403664070248542 22.135999752631346 24-25 21.81597753874893 27.73833675917216 28.697121951759623 21.748563750319285 26-27 21.821045118203333 28.030629281844877 28.392930241643064 21.755395358308725 28-29 21.745949344238824 28.086322695498993 28.480899827578693 21.686828132683488 30-31 22.128630740381414 27.752287541785915 28.454819852012125 21.664261865820546 32-33 21.967440171718675 27.903793498163303 28.269670786030193 21.859095544087822 34-35 21.96705893207084 27.867087383059868 28.44167712875416 21.72417655611513 36-37 22.003656378161114 27.75861769273949 28.364090296998928 21.873635632100463 38-39 21.985452037325626 27.909249972242822 28.412695383958926 21.692602606472626 40-41 22.23378929223668 27.82671963671779 28.304226971235856 21.635264099809678 42-43 22.13820768273121 27.755684287667233 28.182081254438785 21.924026775162776 44-45 22.088798280061944 28.057760682695136 28.034379205274217 21.8190618319687 46-47 22.086954543037255 27.83437427132636 27.868762553640607 22.209908631995777 48-49 22.10368460184744 27.893841834068517 28.032145286107312 21.97032827797673 50-51 22.23768326846172 28.103932435055185 27.698766649020346 21.95961764746275 52-53 21.895940355962072 28.150087071371438 27.800625507332256 22.153347065334238 54-55 21.83235898006087 28.07960794651641 27.943216979336828 22.14481609408589 56-57 21.86996695862416 28.153199840468524 27.947434005635703 22.029399195271615 58-59 21.743158996548402 28.15258046821107 28.11234559483421 21.991914940406318 60-61 21.85571100693427 28.18654165445844 27.980173845102062 21.977573493505226 62-63 21.747064634679642 28.128857634629696 27.810867338175765 22.313210392514897 64-65 21.602168636351255 28.107587837705445 27.972472206252235 22.31777131969106 66-67 21.661004340455346 28.117090436194538 27.750713818685547 22.471191404664573 68-69 21.70107924487467 28.04447613899305 27.81272193834154 22.441722677790736 70-71 21.65483998744306 27.759289051812658 27.90319451737736 22.682676443366923 72-73 21.689698159169783 27.87796876127156 27.853799555367694 22.578533524190966 74-75 21.556660146009886 28.157995851719715 27.559641895235192 22.725702107035215 76-77 21.539932522570517 28.047668529396496 27.734001137505114 22.678397810527873 78-79 21.499521998443814 28.08938025518966 27.659594915802693 22.75150283056383 80-81 21.533971866666523 27.991843668491473 27.577921841134927 22.896262623707074 82-83 21.692098954706175 27.840049601789968 27.76099134394954 22.70686009955431 84-85 21.82733918621618 27.796235796012237 27.59789614004424 22.778528877727343 86-87 21.421404229202963 27.904128485216873 27.757787366578434 22.91667991900173 88-89 21.593432289194656 27.736486212054572 27.70153330911851 22.968548189632262 90-91 21.569118980005193 27.72216011810327 27.826776841754754 22.881944060136778 92-93 21.482033566972216 27.66457322335497 27.68564262620337 23.16775058346944 94-95 21.267877749190593 27.706030686680954 27.839592624459513 23.186498939668944 96-97 21.23349295850567 27.66403823205421 27.833109789279863 23.269359020160252 98-99 21.13869126662982 27.55081654247723 27.611299483908496 23.699192706984455 100-101 21.394604891440455 27.654739002955736 27.415694307411016 23.534961798192793 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 328550.0 1 297893.0 2 197339.5 3 99583.5 4 60817.5 5 46515.5 6 42619.0 7 43991.5 8 46917.5 9 47047.5 10 43883.5 11 37882.0 12 29848.5 13 21895.0 14 15478.0 15 11267.0 16 9437.0 17 9603.0 18 11271.0 19 13614.0 20 16113.5 21 18818.5 22 21650.5 23 24470.0 24 27014.0 25 29658.0 26 32946.5 27 36871.5 28 41056.5 29 46381.0 30 52884.5 31 60857.0 32 71361.5 33 83920.5 34 97597.0 35 111005.0 36 126313.0 37 141667.0 38 151882.0 39 158558.0 40 164166.0 41 167753.5 42 170423.5 43 175114.0 44 178820.5 45 180778.5 46 180807.5 47 177871.5 48 173550.5 49 166654.0 50 156445.5 51 145147.5 52 133827.5 53 121671.0 54 108125.0 55 92304.5 56 76381.5 57 61800.0 58 49081.5 59 39010.5 60 30011.5 61 21901.0 62 15389.0 63 10490.0 64 7002.0 65 4440.0 66 2667.5 67 1592.0 68 992.0 69 581.0 70 331.0 71 203.0 72 139.0 73 92.5 74 66.0 75 43.0 76 26.5 77 23.0 78 18.5 79 12.0 80 8.0 81 6.0 82 3.5 83 3.5 84 2.0 85 1.5 86 2.5 87 1.5 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 22.430522179081976 2 29.55829728674187 3 23.26760413644327 4 29.668135108457232 5 32.81332479879985 6 25.342267277077763 7 24.250958104742942 8 20.22673361402794 9 11.983212218262572 10-11 15.654888862802945 12-13 15.749112031994164 14-15 19.93608143834944 16-17 23.276758826339226 18-19 27.286900598524767 20-21 29.37443752182545 22-23 28.967967444741475 24-25 27.487445524058007 26-27 28.940854058739145 28-29 25.870544563775333 30-31 27.764716348578105 32-33 26.747401433890218 34-35 24.779650674752325 36-37 27.794819774425488 38-39 27.936431383753522 40-41 24.9245014939125 42-43 25.79041334163162 44-45 24.49512623505257 46-47 27.81054516916612 48-49 23.14431113542518 50-51 28.771326182339273 52-53 22.316116240936672 54-55 23.474009170932085 56-57 25.607947082939276 58-59 24.882585564973567 60-61 24.40736302848954 62-63 24.239680855697724 64-65 22.758522167269472 66-67 23.260378206817734 68-69 24.269784281545103 70-71 22.743756538405034 72-73 22.603141609025343 74-75 19.87660363957986 76-77 22.148166441121685 78-79 21.25860190166534 80-81 22.30528196075739 82-83 23.167917684572206 84-85 20.082030451156406 86-87 21.658464359832468 88-89 20.474999206347448 90-91 21.62306376462997 92-93 21.596983972648147 94-95 20.650820932050788 96-97 19.532998227386255 98-99 18.933975121391928 100-101 20.6264207303523 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5417988.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.54154750616138 #Duplication Level Percentage of deduplicated Percentage of total 1 81.44352757011991 53.37934831306379 2 11.028388290767065 14.456352701514072 3 3.528243729367814 6.937396620050297 4 1.5603934373732224 4.090824024155982 5 0.7872980789060214 2.58003672200643 6 0.44567043943769596 1.7525958167098545 7 0.27254942821233735 1.250431790786922 8 0.1847014762704922 0.9684496465152483 9 0.1304007461921519 0.7692000031252634 >10 0.5792697959067408 6.523505425192917 >50 0.027356262944151288 1.2239418163476794 >100 0.011801629911684172 1.289134972903762 >500 2.8508275560042975E-4 0.11961961382589155 >1k 8.55238763097828E-5 0.09910289213257352 >5k 0.0 0.0 >10k+ 2.850795876992593E-5 4.560059641669321 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 244055 4.504531940639219 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 1.845703608055241E-5 0.0 0.0 0.0 3 0.0 1.845703608055241E-5 0.0 0.0 0.0 4 0.0 1.845703608055241E-5 0.0 0.0 0.0 5 0.0 1.845703608055241E-5 0.0 3.691407216110482E-5 0.0 6 0.0 1.845703608055241E-5 0.0 3.691407216110482E-5 0.0 7 0.0 1.845703608055241E-5 0.0 3.691407216110482E-5 0.0 8 0.0 1.845703608055241E-5 0.0 3.691407216110482E-5 0.0 9 0.0 1.845703608055241E-5 0.0 3.691407216110482E-5 0.0 10-11 0.0 1.845703608055241E-5 0.0 3.691407216110482E-5 0.0 12-13 0.0 1.845703608055241E-5 0.0 3.691407216110482E-5 0.0 14-15 0.0 1.845703608055241E-5 0.0 5.537110824165724E-5 0.0 16-17 0.0 1.845703608055241E-5 0.0 7.382814432220964E-5 9.228518040276206E-6 18-19 0.0 2.768555412082862E-5 0.0 8.305666236248585E-5 1.845703608055241E-5 20-21 0.0 3.691407216110482E-5 0.0 1.1997073452359068E-4 1.845703608055241E-5 22-23 0.0 3.691407216110482E-5 0.0 1.661133247249717E-4 1.845703608055241E-5 24-25 0.0 3.691407216110482E-5 0.0 2.1225591492635273E-4 1.845703608055241E-5 26-27 0.0 3.691407216110482E-5 0.0 2.3071295100690517E-4 1.845703608055241E-5 28-29 0.0 3.691407216110482E-5 0.0 5.3525404633602E-4 1.845703608055241E-5 30-31 0.0 3.691407216110482E-5 0.0 0.0018087895358941362 1.845703608055241E-5 32-33 0.0 3.691407216110482E-5 0.0 0.005361768981400476 1.845703608055241E-5 34-35 0.0 3.691407216110482E-5 0.0 0.01075122351692178 1.845703608055241E-5 36-37 0.0 3.691407216110482E-5 0.0 0.01979517119639246 1.845703608055241E-5 38-39 0.0 3.691407216110482E-5 0.0 0.035437509274660633 1.845703608055241E-5 40-41 0.0 3.691407216110482E-5 0.0 0.05183658583223145 1.845703608055241E-5 42-43 0.0 3.691407216110482E-5 0.0 0.0689554867969438 1.845703608055241E-5 44-45 0.0 3.691407216110482E-5 0.0 0.09061481863747206 1.845703608055241E-5 46-47 0.0 3.691407216110482E-5 1.845703608055241E-5 0.1184849431191062 1.845703608055241E-5 48-49 0.0 3.691407216110482E-5 1.845703608055241E-5 0.1492897363375482 1.845703608055241E-5 50-51 0.0 3.691407216110482E-5 1.845703608055241E-5 0.1858162107409614 1.845703608055241E-5 52-53 0.0 3.691407216110482E-5 1.845703608055241E-5 0.22729839933200296 1.845703608055241E-5 54-55 0.0 3.691407216110482E-5 1.845703608055241E-5 0.27512980833475453 1.845703608055241E-5 56-57 0.0 3.691407216110482E-5 1.845703608055241E-5 0.33240199129270864 1.845703608055241E-5 58-59 0.0 3.691407216110482E-5 1.845703608055241E-5 0.39588496689176866 1.845703608055241E-5 60-61 2.768555412082862E-5 3.691407216110482E-5 1.845703608055241E-5 0.4641667718717723 1.845703608055241E-5 62-63 3.691407216110482E-5 4.614259020138103E-5 1.845703608055241E-5 0.5368505799569878 1.845703608055241E-5 64-65 3.691407216110482E-5 5.537110824165724E-5 1.845703608055241E-5 0.6172402006058337 1.845703608055241E-5 66-67 3.691407216110482E-5 5.537110824165724E-5 1.845703608055241E-5 0.7107435453899122 1.845703608055241E-5 68-69 3.691407216110482E-5 5.537110824165724E-5 1.845703608055241E-5 0.8164931336134373 1.845703608055241E-5 70-71 3.691407216110482E-5 5.537110824165724E-5 1.845703608055241E-5 0.9333261720033341 1.845703608055241E-5 72-73 3.691407216110482E-5 7.382814432220964E-5 1.845703608055241E-5 1.0591016443742585 1.845703608055241E-5 74-75 3.691407216110482E-5 7.382814432220964E-5 1.845703608055241E-5 1.1908756534713625 1.845703608055241E-5 76-77 3.691407216110482E-5 7.382814432220964E-5 1.845703608055241E-5 1.3393255946672453 1.845703608055241E-5 78-79 3.691407216110482E-5 8.305666236248585E-5 1.845703608055241E-5 1.5083920451651056 1.845703608055241E-5 80-81 3.691407216110482E-5 9.228518040276205E-5 1.845703608055241E-5 1.6919472689861994 1.845703608055241E-5 82-83 3.691407216110482E-5 9.228518040276205E-5 1.845703608055241E-5 1.883651274236857 1.845703608055241E-5 84-85 3.691407216110482E-5 9.228518040276205E-5 1.845703608055241E-5 2.0837993734943674 1.845703608055241E-5 86-87 3.691407216110482E-5 9.228518040276205E-5 1.845703608055241E-5 2.3018046551598124 1.845703608055241E-5 88-89 3.691407216110482E-5 9.228518040276205E-5 1.845703608055241E-5 2.5399465631891394 1.845703608055241E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3270 0.0 47.857655 1 GTATCAA 6660 0.0 45.146362 1 ATCAACG 7480 0.0 38.90257 3 CAACGCA 7710 0.0 38.189285 5 TCAACGC 7940 0.0 37.28081 4 TATCAAC 8100 0.0 36.038364 2 AACGCAG 8695 0.0 33.689224 6 ACGCAGA 9740 0.0 28.872427 7 CGCAGAG 9885 0.0 28.075338 8 GTACATG 9125 0.0 25.472095 1 TACATGG 9315 0.0 24.233023 2 GCAGAGT 11320 0.0 23.391241 9 ACATGGG 9440 0.0 22.651548 3 GTGTAGC 2380 0.0 22.205454 1 GTGGTAT 2135 0.0 19.706764 1 GTATAAT 2530 0.0 19.67209 1 AGAGTAC 9740 0.0 19.532694 10-11 GTACTAG 1160 0.0 18.577644 1 CAGAGTA 10755 0.0 18.540771 10-11 GAGTACA 6670 0.0 18.385328 1 >>END_MODULE