FastQCFastQC Report
Sat 18 Jun 2016
SRR1931003_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931003_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5417988
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70330.1298083347545251No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51300.046.5075381
GTATCAA102650.043.93841
CAACGCA125800.035.1838765
ATCAACG126000.035.112683
TCAACGC130850.033.9198534
TATCAAC132650.033.641492
AACGCAG138450.032.7566576
ACGCAGA156750.028.0242567
CGCAGAG157700.027.8253568
GCAGAGT185400.023.0281729
CTAACGC3850.022.2175713
GTACATG149150.020.903431
TACATGG147300.020.6165052
TAACGCC4900.020.365924
ACATGGG148300.019.8991833
AGAGTAC169100.019.81961410-11
GAGTACT108550.018.81351712-13
GGACCGT5100.018.6034936
GTGGTAT32200.018.4497761
CAGAGTA182950.017.64502710-11
Produced by FastQC (version 0.11.5)