##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931003_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5417988 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.345768023111162 31.0 31.0 34.0 30.0 34.0 2 31.501378740595218 31.0 31.0 34.0 30.0 34.0 3 31.584914547614353 31.0 31.0 34.0 30.0 34.0 4 35.354177787030906 37.0 35.0 37.0 33.0 37.0 5 35.22173101896867 37.0 35.0 37.0 33.0 37.0 6 35.24189717659028 37.0 35.0 37.0 32.0 37.0 7 35.12262227232692 37.0 35.0 37.0 32.0 37.0 8 35.12089672402375 37.0 35.0 37.0 32.0 37.0 9 36.62672194918113 39.0 35.0 39.0 32.0 39.0 10-11 36.567295276401495 38.0 35.0 39.0 32.0 39.0 12-13 36.450380934767665 38.0 35.0 39.0 32.0 39.0 14-15 37.53299951937878 39.0 36.0 41.0 32.0 41.0 16-17 37.48675163178656 39.0 36.0 41.0 32.0 41.0 18-19 37.48739181408301 39.0 36.0 41.0 32.0 41.0 20-21 37.45768170767451 39.0 36.0 41.0 32.0 41.0 22-23 37.35526952071507 39.0 36.0 40.5 32.0 41.0 24-25 37.23405893479277 39.0 36.0 40.0 31.0 41.0 26-27 36.99380628011727 39.0 36.0 40.0 31.0 41.0 28-29 36.815086153753015 39.0 36.0 40.0 30.5 41.0 30-31 36.61137593881714 38.0 35.0 40.0 30.0 41.0 32-33 36.485762426937825 38.0 35.0 40.0 30.0 41.0 34-35 36.60111188876756 38.0 35.0 40.0 30.0 41.0 36-37 36.70153689893739 39.0 35.0 40.0 30.0 41.0 38-39 36.70051585570141 39.0 35.0 40.0 30.0 41.0 40-41 36.606819173464395 39.0 35.0 40.0 30.0 41.0 42-43 36.49291683554855 39.0 35.0 40.0 30.0 41.0 44-45 36.36594756208393 39.0 35.0 40.0 30.0 41.0 46-47 36.1907271666161 38.0 35.0 40.0 30.0 41.0 48-49 36.0171519390593 38.0 35.0 40.0 29.0 41.0 50-51 35.77485064566403 38.0 34.5 40.0 28.5 41.0 52-53 35.57481596489324 38.0 34.0 40.0 28.0 41.0 54-55 35.42822390894923 38.0 34.0 40.0 28.0 41.0 56-57 35.260562869463726 38.0 34.0 40.0 27.0 41.0 58-59 35.05859167646735 38.0 34.0 40.0 27.0 41.0 60-61 34.75513354773027 37.5 33.0 40.0 26.0 41.0 62-63 34.50637709053619 37.0 33.0 40.0 26.0 41.0 64-65 34.19244948493795 37.0 33.0 39.5 26.0 41.0 66-67 33.86895984265746 36.0 32.0 39.0 26.0 41.0 68-69 33.51455669521601 36.0 32.0 39.0 25.5 40.0 70-71 33.14113698664522 35.5 32.0 38.5 25.0 40.0 72-73 32.7113920333526 35.0 31.0 38.0 24.0 40.0 74-75 32.190633404872806 35.0 31.0 37.0 22.0 39.0 76-77 30.772105161547053 33.5 29.5 35.5 21.5 38.5 78-79 31.51945528487697 34.0 30.5 36.0 22.5 39.0 80-81 31.496830188623527 34.0 31.0 36.0 23.0 37.5 82-83 31.238661750450536 34.0 31.0 35.5 22.5 37.0 84-85 30.922165940566867 34.0 31.0 35.0 21.5 37.0 86-87 30.599917718533156 34.0 30.0 35.0 20.0 36.0 88-89 30.276291586470844 34.0 30.0 35.0 20.0 36.0 90-91 29.96783787634819 34.0 30.0 35.0 18.5 35.5 92-93 29.676227780497115 34.0 29.5 35.0 17.0 35.0 94-95 29.318469974462843 34.0 29.0 35.0 8.5 35.0 96-97 28.91371354089378 34.0 29.0 35.0 2.0 35.0 98-99 28.442047767547656 34.0 29.0 35.0 2.0 35.0 100-101 27.325210853180185 33.0 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3397992386403317 1101 2 0.39012518915553684 1101 3 0.27223338762791016 1101 4 0.2270712768961829 1101 5 0.26968186653119375 1101 6 0.2699442376182404 1101 7 0.33075993401210013 1101 8 0.3366608088184435 1101 9 0.4424499025506776 1101 10-11 0.4649772800268792 1101 12-13 0.4460883700405134 1101 14-15 0.5108496100614985 1101 16-17 0.5504669055224127 1101 18-19 0.40625031415576274 1101 20-21 0.42452976765352446 1101 22-23 0.4858395704994294 1101 24-25 0.4501886121570493 1101 26-27 0.48287803650340066 1101 28-29 0.4628324423851282 1101 30-31 0.4796112351622668 1101 32-33 0.4131463505108641 1101 34-35 0.43778875187113186 1101 36-37 0.4251909235462037 1101 38-39 0.47600385095707765 1101 40-41 0.5236067560796727 1101 42-43 0.46704579656795175 1101 44-45 0.3984313638217216 1101 46-47 0.3982290592588811 1101 48-49 0.28945701508956745 1101 50-51 0.3256815710739005 1101 52-53 0.18137714609645883 1101 54-55 0.20219072812049177 1101 56-57 0.2960288867674876 1101 58-59 0.2403188785032313 1101 60-61 0.24285453222834263 1101 62-63 0.047400756007796474 1101 64-65 0.18893589582286552 1101 66-67 0.10758415086178985 1101 68-69 0.13958667553268356 1101 70-71 0.09817923226075465 1101 72-73 0.12018655261164923 1101 74-75 0.13286045450514905 1101 76-77 0.1371418756396494 1101 78-79 0.1999272144807236 1101 80-81 0.19183235385665043 1101 82-83 0.23121682581998826 1101 84-85 0.04815696899750321 1101 86-87 0.08696545590435534 1101 88-89 0.06729555648692198 1101 90-91 0.04923149119488812 1101 92-93 -0.03635915781155319 1101 94-95 0.07700966332544112 1101 96-97 0.19515461405470447 1101 98-99 0.2507482696688861 1101 100-101 0.4547721635422093 1102 1 0.25489871774262696 1102 2 0.3674155100130996 1102 3 0.39668122747697154 1102 4 0.15346965905688847 1102 5 0.2508069385298626 1102 6 0.2963482474963115 1102 7 0.3781212635814484 1102 8 0.3581834211367081 1102 9 0.5121572217309591 1102 10-11 0.41198591659719597 1102 12-13 0.44728916549725284 1102 14-15 0.5399720595166286 1102 16-17 0.480300034845321 1102 18-19 0.499344818791954 1102 20-21 0.4536912227768397 1102 22-23 0.42184717786516757 1102 24-25 0.46150877245296584 1102 26-27 0.40938778069843806 1102 28-29 0.4398414114404474 1102 30-31 0.38893300392249586 1102 32-33 0.5702481346607939 1102 34-35 0.4560147550049436 1102 36-37 0.48934550481473593 1102 38-39 0.44853091544022305 1102 40-41 0.4992853763541447 1102 42-43 0.5669728338254814 1102 44-45 0.6037212614422316 1102 46-47 0.5449008980657908 1102 48-49 0.449286709188371 1102 50-51 0.4146325495677061 1102 52-53 0.36605580139087124 1102 54-55 0.4533778971429214 1102 56-57 0.39481968380080446 1102 58-59 0.491283698183949 1102 60-61 0.3971379609716976 1102 62-63 0.0699790763235697 1102 64-65 0.1547467633675197 1102 66-67 0.16412564328609847 1102 68-69 0.3273300862319104 1102 70-71 0.3660432832334308 1102 72-73 0.22094456389897488 1102 74-75 0.26776855851353787 1102 76-77 0.28335906846609404 1102 78-79 0.3164924005490022 1102 80-81 0.24712910557361667 1102 82-83 0.16483871706823194 1102 84-85 0.28045742315293154 1102 86-87 0.17975050421756222 1102 88-89 0.1949404644178756 1102 90-91 0.1919876719070217 1102 92-93 0.17140526621201602 1102 94-95 0.2696377078626213 1102 96-97 0.2913181323564338 1102 98-99 0.3977303142526729 1102 100-101 0.5540282351747408 1103 1 0.33831559278176826 1103 2 0.39002955187723387 1103 3 0.3419397491487004 1103 4 0.2436560356509787 1103 5 0.27456313848963987 1103 6 0.29891819511350803 1103 7 0.31613864261785807 1103 8 0.36013739140016554 1103 9 0.39555466701568065 1103 10-11 0.40891161078649674 1103 12-13 0.47523808522763034 1103 14-15 0.46630150888192645 1103 16-17 0.4859599617421182 1103 18-19 0.47879344219686004 1103 20-21 0.45821864964306513 1103 22-23 0.4345630063777719 1103 24-25 0.4889263649292772 1103 26-27 0.5286421426515702 1103 28-29 0.5247831245165102 1103 30-31 0.4483183573097591 1103 32-33 0.6682542020644604 1103 34-35 0.5255751639962583 1103 36-37 0.4394967220417314 1103 38-39 0.4396265136417199 1103 40-41 0.5577454102449266 1103 42-43 0.5114635894531077 1103 44-45 0.48827105759021805 1103 46-47 0.41050877233637095 1103 48-49 0.3461924456044727 1103 50-51 0.3935990049355098 1103 52-53 0.4599578595211895 1103 54-55 0.42759849964709673 1103 56-57 0.3457596541475283 1103 58-59 0.3790589037453316 1103 60-61 0.3236849841522016 1103 62-63 0.31388578970809533 1103 64-65 0.22619607600402958 1103 66-67 0.28309432368495635 1103 68-69 0.2657175629898987 1103 70-71 0.2787433480460564 1103 72-73 0.26486479857458534 1103 74-75 0.33832431632776405 1103 76-77 0.36467065241616936 1103 78-79 0.4148197573558221 1103 80-81 0.2965784989502147 1103 82-83 0.3126118747188009 1103 84-85 0.36202888925857124 1103 86-87 0.31166881182512896 1103 88-89 0.2900700993784433 1103 90-91 0.2736840411897248 1103 92-93 0.1637529707238805 1103 94-95 0.27613974116938067 1103 96-97 0.4982558613636954 1103 98-99 0.33206763631117653 1103 100-101 0.4302426031807052 1104 1 0.30142953097477587 1104 2 0.36678030689462915 1104 3 0.3542299860678533 1104 4 0.13653036166105892 1104 5 0.2751463818246265 1104 6 0.251128382584902 1104 7 0.2534998918186915 1104 8 0.2927110440945384 1104 9 0.39817995394675165 1104 10-11 0.40181133200243124 1104 12-13 0.4459945128601106 1104 14-15 0.5239724251181741 1104 16-17 0.5506600656336431 1104 18-19 0.5403055965271051 1104 20-21 0.5289404843129688 1104 22-23 0.5259267891132353 1104 24-25 0.40026266124045407 1104 26-27 0.5156788410572588 1104 28-29 0.5901600906174664 1104 30-31 0.5631755536082323 1104 32-33 0.5763042152045728 1104 34-35 0.4664458980538413 1104 36-37 0.503931127264508 1104 38-39 0.5168188317834463 1104 40-41 0.4914827336410852 1104 42-43 0.4857002802379782 1104 44-45 0.5659895916879307 1104 46-47 0.45487555827779147 1104 48-49 0.38756283218766896 1104 50-51 0.39424881775890697 1104 52-53 0.2984607472885443 1104 54-55 0.4164946542703376 1104 56-57 0.5372536504711292 1104 58-59 0.6253085007400614 1104 60-61 0.49752154033701146 1104 62-63 0.4331219131156061 1104 64-65 0.4561004418618282 1104 66-67 0.4877173775371446 1104 68-69 0.4596559323557159 1104 70-71 0.5047250701918031 1104 72-73 0.47698185183988784 1104 74-75 0.5361311984510806 1104 76-77 0.4879072528969637 1104 78-79 0.6567756786675645 1104 80-81 0.6317264582872504 1104 82-83 0.4827780174866483 1104 84-85 0.5932714353907258 1104 86-87 0.6530529178054287 1104 88-89 0.6480962878184009 1104 90-91 0.5435308402899253 1104 92-93 0.5726029923859173 1104 94-95 0.7551125042881388 1104 96-97 0.7932100743642998 1104 98-99 0.8821926427718161 1104 100-101 0.9634329078568804 1105 1 0.24834554893220329 1105 2 0.2881600680153227 1105 3 0.27678051060644293 1105 4 0.211185310893228 1105 5 0.20213304821164257 1105 6 0.23400563597910207 1105 7 0.20789311514251807 1105 8 0.2538395200783654 1105 9 0.3256414051465697 1105 10-11 0.3480046077485994 1105 12-13 0.4232048262399388 1105 14-15 0.3781334152473832 1105 16-17 0.41613878399430604 1105 18-19 0.3724114712931552 1105 20-21 0.3843079590813687 1105 22-23 0.36676129801652024 1105 24-25 0.41051947388128696 1105 26-27 0.35382512908709174 1105 28-29 0.4361313392322401 1105 30-31 0.3663161059553772 1105 32-33 0.48060139853711803 1105 34-35 0.4848883323303781 1105 36-37 0.4939153899519724 1105 38-39 0.4142947131706407 1105 40-41 0.2810145930690737 1105 42-43 0.3954788685914892 1105 44-45 0.4489392800409533 1105 46-47 0.3758341242204182 1105 48-49 0.26116091080106685 1105 50-51 0.3475971368390276 1105 52-53 0.28041828457235596 1105 54-55 0.43151009850968336 1105 56-57 0.4867227604006672 1105 58-59 0.5338809321720106 1105 60-61 0.47320275448274884 1105 62-63 0.33450791834946614 1105 64-65 0.2760715727479379 1105 66-67 0.3214477173665955 1105 68-69 0.2513351693341974 1105 70-71 0.24067519131936166 1105 72-73 0.28719890452983066 1105 74-75 0.4478561642108261 1105 76-77 0.4815837228150741 1105 78-79 0.5335220312796167 1105 80-81 0.45807814212226106 1105 82-83 0.5612481772986193 1105 84-85 0.6355872092390982 1105 86-87 0.5151393529860933 1105 88-89 0.5814261005280876 1105 90-91 0.5966234990467996 1105 92-93 0.5615585494437703 1105 94-95 0.5668315492827674 1105 96-97 0.5577990955491572 1105 98-99 0.6362273305277633 1105 100-101 0.6226383024760338 1106 1 0.24601345300987987 1106 2 0.2992540225956084 1106 3 0.2998723788640447 1106 4 0.18259996471609696 1106 5 0.20892581838430857 1106 6 0.14691684899193547 1106 7 0.26407069296661234 1106 8 0.25493046726684554 1106 9 0.42070752575504855 1106 10-11 0.29482171142711167 1106 12-13 0.35320670498140316 1106 14-15 0.4006669926714608 1106 16-17 0.39729311892364905 1106 18-19 0.38275690826583997 1106 20-21 0.36575130060499816 1106 22-23 0.35191555876169645 1106 24-25 0.3670169541098147 1106 26-27 0.39557119822007536 1106 28-29 0.43282797461068157 1106 30-31 0.42096710322367414 1106 32-33 0.4691547941934999 1106 34-35 0.49994351657979763 1106 36-37 0.43356281412837916 1106 38-39 0.45334691636546154 1106 40-41 0.4557737397275261 1106 42-43 0.466064199675138 1106 44-45 0.42928237080194975 1106 46-47 0.38511290412434107 1106 48-49 0.4504341525577047 1106 50-51 0.4542212755289512 1106 52-53 0.4351224237340219 1106 54-55 0.5100943951703272 1106 56-57 0.6082909140310591 1106 58-59 0.578431486587121 1106 60-61 0.5824691693408184 1106 62-63 0.4767772272149031 1106 64-65 0.4920241806772694 1106 66-67 0.5043145087917935 1106 68-69 0.42613884787881773 1106 70-71 0.5039160640318912 1106 72-73 0.6141848272902521 1106 74-75 0.8082046886257714 1106 76-77 0.7166847515337302 1106 78-79 0.7235986954805114 1106 80-81 0.6259718926777644 1106 82-83 0.6490105949959784 1106 84-85 0.7316071371588535 1106 86-87 0.6442182255102331 1106 88-89 0.6839414814443678 1106 90-91 0.6201415137758985 1106 92-93 0.5735889110546033 1106 94-95 0.6889040819023364 1106 96-97 0.6787432986387536 1106 98-99 0.7168654255566409 1106 100-101 0.9048628967447172 1107 1 0.1399713449061295 1107 2 0.14734210724298435 1107 3 0.13901924163619128 1107 4 0.05313498080570156 1107 5 0.146536144676503 1107 6 0.21880111540171043 1107 7 0.15714032111547027 1107 8 0.1835056497023828 1107 9 0.14159307586574243 1107 10-11 0.13984784902506675 1107 12-13 0.19675091050018523 1107 14-15 0.24548951706216116 1107 16-17 0.2634291037815544 1107 18-19 0.2014057734757415 1107 20-21 0.20754531598076653 1107 22-23 0.21219345023582292 1107 24-25 0.17126147388280089 1107 26-27 0.19233289493649863 1107 28-29 0.22821393289756742 1107 30-31 0.24115833923242036 1107 32-33 0.3039619789989416 1107 34-35 0.25748615271202624 1107 36-37 0.23354558253531366 1107 38-39 0.2550229186961772 1107 40-41 0.2735009182313064 1107 42-43 0.29126099487019275 1107 44-45 0.29019228333992686 1107 46-47 0.28495608607443046 1107 48-49 0.278845732851579 1107 50-51 0.2666133688961452 1107 52-53 0.25113613664483125 1107 54-55 0.24523513115382656 1107 56-57 0.2720301546499826 1107 58-59 0.28791828512144946 1107 60-61 0.3479185890371497 1107 62-63 0.20141858620416286 1107 64-65 0.07552219406906602 1107 66-67 0.2024689795459551 1107 68-69 0.20519668829135185 1107 70-71 0.1914749442949315 1107 72-73 0.11525436196939154 1107 74-75 0.22150669453095162 1107 76-77 0.2169401801262616 1107 78-79 0.3051410059763313 1107 80-81 0.35559978524488756 1107 82-83 0.3648746508426193 1107 84-85 0.3014653119517341 1107 86-87 0.3264372566967033 1107 88-89 0.3112123393229247 1107 90-91 0.31697320724349964 1107 92-93 0.2642616894341536 1107 94-95 0.4754610422674119 1107 96-97 0.4988312656952054 1107 98-99 0.6164396408822057 1107 100-101 0.8081715736734552 1108 1 0.059142993717326675 1108 2 0.08013295525525166 1108 3 0.029629448456489627 1108 4 0.04335990178242355 1108 5 0.005441811615128245 1108 6 0.10112495702524171 1108 7 0.1133862584942591 1108 8 0.06581754309563337 1108 9 0.15889874993763442 1108 10-11 0.14051962489767078 1108 12-13 0.12312992798115374 1108 14-15 0.05487097323043599 1108 16-17 0.08404287723775639 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-1.9601347928870148 2316 78-79 -1.6637553761334232 2316 80-81 -1.3543972100477397 2316 82-83 -1.0540176137051702 2316 84-85 -1.0672866216331052 2316 86-87 -0.9333190281495582 2316 88-89 -0.8783408495618836 2316 90-91 -0.8909098094827037 2316 92-93 -0.8176099094040232 2316 94-95 -1.2886934664629308 2316 96-97 -1.1841607551374302 2316 98-99 -1.2746933563690455 2316 100-101 -1.6967784035130258 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 20.0 4 60.0 5 102.0 6 220.0 7 628.0 8 1537.0 9 2909.0 10 4990.0 11 7787.0 12 10876.0 13 14271.0 14 17633.0 15 21405.0 16 24872.0 17 28119.0 18 25322.0 19 21908.0 20 22452.0 21 24437.0 22 27633.0 23 32856.0 24 41211.0 25 53632.0 26 70098.0 27 92071.0 28 117856.0 29 149185.0 30 185236.0 31 225805.0 32 275805.0 33 335516.0 34 417676.0 35 524427.0 36 693153.0 37 948695.0 38 864874.0 39 132701.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.35835230956962 21.03771664241609 14.751978730618537 23.851952317395757 2 15.031511191581362 21.67799794706105 40.35856105259913 22.931929808758454 3 18.79378783532913 25.8843763047063 30.333750101054175 24.988085758910394 4 12.807301258444308 17.832629329016434 38.87261197957928 30.487457432959975 5 12.847278427591313 38.22302048458447 35.792678278713865 13.137022809110356 6 30.175645381049183 38.74545447162605 17.900605430602873 13.178294716721897 7 26.50262911298975 32.892308527133785 22.721446175489163 17.883616184387304 8 24.52543441206138 36.735761002075506 20.682416151481508 18.056388434381606 9 25.02072725151846 17.15120077785333 21.15002838692149 36.678043583706724 10-11 24.01110892087616 27.215139642243578 29.033388409128996 19.74036302775126 12-13 24.721529468134666 24.36741277389319 29.123643315562898 21.787414442409247 14-15 21.760614826020287 26.3224281781355 26.75448893574515 25.162468060099062 16-17 21.45939599718567 29.294204785983286 27.48146544436791 21.764933772463134 18-19 21.298930525501348 28.47843147677699 29.236360434906832 20.98627756281483 20-21 22.096375997879655 27.884604026439337 29.08356386171398 20.935456113967028 22-23 21.741387819709463 27.536191626156388 28.887067582188592 21.83535297194556 24-25 21.5828152103174 27.7189507330627 29.1709010449332 21.5273330116867 26-27 21.61196108667382 28.0393526160032 28.87843309380656 21.470253203516425 28-29 21.507857032708035 28.076033127103354 28.891124654287697 21.524985185900906 30-31 21.947282274390403 27.77305194940036 28.89875688204846 21.380908894160775 32-33 21.857607492480863 27.852574303413576 28.671482878222076 21.618335325883482 34-35 21.825319104986818 27.874972241507283 28.847216333124802 21.452492320381094 36-37 21.82663788701572 27.788377833417943 28.878910532279882 21.50607374728645 38-39 21.751659983665483 27.929290925115012 28.89169846667805 21.427350624541457 40-41 21.97290777619342 27.799785581188587 28.78347825260389 21.443828390014104 42-43 21.888695478819137 27.744928831042255 28.697620071658125 21.668755618480485 44-45 21.844028040450368 27.996833285280093 28.518872371946113 21.640266302323425 46-47 21.869204767013926 27.819702580786583 28.35016555336356 21.960927098835935 48-49 21.936894489735455 27.844745714530955 28.503756851720418 21.714602944013173 50-51 21.931474641693438 27.976042595789814 28.246228178020694 21.84625458449605 52-53 21.981151930363918 27.97012217689624 28.144025756540163 21.90470013619968 54-55 21.932191350042377 27.93068647664871 28.27996220094749 21.85715997236143 56-57 21.930533501856665 28.011909228129 28.20009053745191 21.85746673256243 58-59 21.752194278722595 28.02554419921426 28.484752379619728 21.73750914244342 60-61 21.89818972269419 28.075777313799172 28.33697548429397 21.689057479212668 62-63 21.867444257362713 28.017639539979978 28.160676587528073 21.954239615129236 64-65 21.584184263209792 28.00590683988784 28.327798716003315 22.082110180899054 66-67 21.76446762385991 27.98089782940309 28.215456186537963 22.03917836019904 68-69 21.797923781702888 27.944982028385272 28.204407551001605 22.052686638910238 70-71 21.720510851671364 27.66434573203237 28.28678282717422 22.32836058912205 72-73 21.807925709655127 27.80016639644114 28.255718479627156 22.13618941427658 74-75 21.66821992252136 28.063044142726305 28.027916592983022 22.240819341769313 76-77 21.592712036012564 27.967155813365917 28.110491000004068 22.329641150617455 78-79 21.65020868232545 27.94698546953039 28.10200557083012 22.300800277314046 80-81 21.62037502308284 27.9612730670333 28.036937707393694 22.38141420249017 82-83 21.73026223018582 27.85809972262766 28.207325302307794 22.204312744878727 84-85 21.85720049582982 27.771333196012986 28.01112700877152 22.360339299385675 86-87 21.435124994739745 27.87484948287076 28.18669956448778 22.503325957901716 88-89 21.589718611202986 27.72628158370346 28.129780375874127 22.55421942921943 90-91 21.57030967719905 27.741574656574358 28.223138875058083 22.46497679116851 92-93 21.536887010607423 27.692461623421856 28.17575420086566 22.594897165105056 94-95 21.278547923559596 27.74957517675628 28.255348555324105 22.716528344360018 96-97 21.302923143127064 27.721017761856736 28.243758082453695 22.73230101256251 98-99 21.196558574880562 27.685000409746202 28.185370657889976 22.933070357483256 100-101 21.250628462078545 27.67026246643588 28.073631761458312 23.005477310027263 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 114.0 1 90.0 2 101.0 3 144.0 4 134.5 5 145.0 6 261.0 7 561.0 8 947.0 9 1150.0 10 1092.5 11 1043.0 12 1153.0 13 1297.0 14 1511.0 15 1806.5 16 2104.0 17 2448.0 18 2855.0 19 3288.5 20 3825.5 21 4490.5 22 5330.5 23 6891.5 24 8865.5 25 11149.0 26 15155.5 27 20700.0 28 26570.0 29 34985.5 30 45349.5 31 58091.5 32 75390.5 33 95726.0 34 118741.5 35 140120.0 36 166139.0 37 192640.0 38 206941.0 39 216561.5 40 226064.5 41 231435.5 42 235423.5 43 244545.5 44 252551.0 45 257232.5 46 258251.0 47 254645.0 48 249972.5 49 242517.0 50 227137.5 51 207830.0 52 191235.0 53 174349.5 54 155017.0 55 131551.0 56 106062.5 57 82333.0 58 63576.5 59 49515.0 60 36316.5 61 24693.5 62 16137.0 63 10090.0 64 6033.5 65 3471.5 66 1890.0 67 988.0 68 545.5 69 300.5 70 139.0 71 89.0 72 63.0 73 41.5 74 29.0 75 21.0 76 12.0 77 8.0 78 6.5 79 3.5 80 2.0 81 1.5 82 1.5 83 0.5 84 1.0 85 2.0 86 1.0 87 0.0 88 0.0 89 0.5 90 0.5 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.004558887911896446 2 0.007179787035334889 3 0.0018826176802163462 4 0.16919564975042395 5 0.002011816932780213 6 4.7988293809436266E-4 7 1.845703608055241E-5 8 6.090821906582296E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 4.060547937721531E-4 24-25 6.27539226738782E-4 26-27 0.0028146980022842427 28-29 1.384277706041431E-4 30-31 0.006653761507039144 32-33 0.021114849276151957 34-35 0.013418265230561603 36-37 7.290529251818203E-4 38-39 2.3994146904718133E-4 40-41 0.0015965336209677835 42-43 0.011037307576170342 44-45 0.007124415927093231 46-47 0.010160598362344102 48-49 0.010206740952545482 50-51 0.1886955083695276 52-53 0.11223723640583921 54-55 0.01699893023018877 56-57 0.006284620785428096 58-59 0.017460356132202582 60-61 0.1214380688919946 62-63 0.23523492484664046 64-65 0.24118731898261866 66-67 0.2505727218295795 68-69 0.24647525981969692 70-71 0.23297393792677282 72-73 0.1590535084241604 74-75 0.2735609602679076 76-77 0.21096392240071404 78-79 0.005601710450447657 80-81 2.768555412082862E-5 82-83 0.0 84-85 0.0 86-87 0.0 88-89 7.382814432220964E-5 90-91 0.006100050424622572 92-93 0.018078666840901085 94-95 0.0027039557858009283 96-97 2.768555412082862E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5417988.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.1696035565419 #Duplication Level Percentage of deduplicated Percentage of total 1 65.36547179679644 29.525324473476154 2 17.12746492176577 15.472816008894755 3 6.9163686420582 9.372288888380007 4 3.4611071255567047 6.253473469124746 5 2.038074106977822 4.60294997155207 6 1.2309503509665733 3.3360923610567736 7 0.8313771655815215 2.6287083882695175 8 0.5625734609959487 2.0328976163694947 9 0.4176246911711327 1.697754756206098 >10 1.8726927933495514 14.403789138370731 >50 0.10777447615045903 3.380947113402083 >100 0.06425256671572173 5.447487346604816 >500 0.0032009308606263886 0.9721276290591871 >1k 9.848963571221455E-4 0.650090347348081 >5k 8.207469641877328E-5 0.22325249188540813 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7033 0.1298083347545251 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 3.691407216110482E-5 0.0 0.0 0.0 0.0 7 3.691407216110482E-5 0.0 0.0 0.0 0.0 8 3.691407216110482E-5 0.0 0.0 0.0 0.0 9 3.691407216110482E-5 0.0 0.0 0.0 0.0 10-11 3.691407216110482E-5 0.0 0.0 1.845703608055241E-5 0.0 12-13 3.691407216110482E-5 0.0 0.0 1.845703608055241E-5 0.0 14-15 3.691407216110482E-5 0.0 0.0 1.845703608055241E-5 0.0 16-17 5.537110824165724E-5 0.0 0.0 1.845703608055241E-5 0.0 18-19 5.537110824165724E-5 0.0 0.0 2.768555412082862E-5 0.0 20-21 5.537110824165724E-5 0.0 0.0 4.614259020138103E-5 0.0 22-23 5.537110824165724E-5 0.0 0.0 9.228518040276207E-5 0.0 24-25 5.537110824165724E-5 0.0 0.0 1.476562886444193E-4 0.0 26-27 5.537110824165724E-5 0.0 0.0 2.2148443296662895E-4 0.0 28-29 5.537110824165724E-5 0.0 0.0 7.290529251818203E-4 0.0 30-31 5.537110824165724E-5 0.0 0.0 0.0029346687368078336 0.0 32-33 5.537110824165724E-5 0.0 0.0 0.009523830617565045 0.0 34-35 5.537110824165724E-5 0.0 0.0 0.019149174933573127 0.0 36-37 6.459962628193344E-5 0.0 0.0 0.03607427701943969 0.0 38-39 7.382814432220964E-5 0.0 0.0 0.0653102221710347 0.0 40-41 7.382814432220964E-5 0.0 0.0 0.10036013368800374 0.0 42-43 7.382814432220964E-5 0.0 0.0 0.1376433465707196 0.0 44-45 7.382814432220964E-5 0.0 0.0 0.18417353452979224 0.0 46-47 7.382814432220964E-5 0.0 0.0 0.24030138125075212 0.0 48-49 7.382814432220964E-5 0.0 0.0 0.30271384875714014 0.0 50-51 7.382814432220964E-5 0.0 0.0 0.3708295404124188 0.0 52-53 7.382814432220964E-5 0.0 0.0 0.4404863945804236 0.0 54-55 7.382814432220964E-5 0.0 0.0 0.5221772362729485 0.0 56-57 7.382814432220964E-5 0.0 0.0 0.6198610997292722 0.0 58-59 7.382814432220964E-5 0.0 0.0 0.728471528545283 0.0 60-61 7.382814432220964E-5 0.0 0.0 0.8474086690483627 0.0 62-63 7.382814432220964E-5 0.0 0.0 0.9742454209939189 0.0 64-65 7.382814432220964E-5 0.0 0.0 1.1124517071650954 0.0 66-67 7.382814432220964E-5 0.0 0.0 1.2739784584240497 0.0 68-69 7.382814432220964E-5 0.0 0.0 1.4575798248353449 0.0 70-71 8.305666236248585E-5 0.0 0.0 1.658290863693312 0.0 72-73 1.1074221648331447E-4 0.0 0.0 1.8735368184647143 0.0 74-75 1.1074221648331447E-4 1.845703608055241E-5 0.0 2.1038344861598066 0.0 76-77 1.1074221648331447E-4 1.845703608055241E-5 0.0 2.3665888518025513 0.0 78-79 1.1074221648331447E-4 1.845703608055241E-5 0.0 2.6621967416686783 0.0 80-81 1.1074221648331447E-4 1.845703608055241E-5 0.0 2.9866160648565483 0.0 82-83 1.1074221648331447E-4 1.845703608055241E-5 0.0 3.3274806071921903 0.0 84-85 1.1074221648331447E-4 1.845703608055241E-5 0.0 3.6870052130052704 0.0 86-87 1.1074221648331447E-4 1.845703608055241E-5 0.0 4.083314691726892 0.0 88-89 1.1074221648331447E-4 1.845703608055241E-5 0.0 4.520349620560252 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5130 0.0 46.507538 1 GTATCAA 10265 0.0 43.9384 1 CAACGCA 12580 0.0 35.183876 5 ATCAACG 12600 0.0 35.11268 3 TCAACGC 13085 0.0 33.919853 4 TATCAAC 13265 0.0 33.64149 2 AACGCAG 13845 0.0 32.756657 6 ACGCAGA 15675 0.0 28.024256 7 CGCAGAG 15770 0.0 27.825356 8 GCAGAGT 18540 0.0 23.028172 9 CTAACGC 385 0.0 22.217571 3 GTACATG 14915 0.0 20.90343 1 TACATGG 14730 0.0 20.616505 2 TAACGCC 490 0.0 20.36592 4 ACATGGG 14830 0.0 19.899183 3 AGAGTAC 16910 0.0 19.819614 10-11 GAGTACT 10855 0.0 18.813517 12-13 GGACCGT 510 0.0 18.603493 6 GTGGTAT 3220 0.0 18.449776 1 CAGAGTA 18295 0.0 17.645027 10-11 >>END_MODULE